Difference between revisions of "Plasmids"

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[[File:Actinobase-icons-trans_Plasmids.png|150px|Plasmids|link=Plasmids]]
 
[[File:Actinobase-icons-trans_Plasmids.png|150px|Plasmids|link=Plasmids]]
  
Various plasmids have been developed for use in <em>Streptomyces</em> (most will also contain an origin of replication that functions in <em>Escherichia coli</em>, for cloning, general maintenance, and [[intergenic conjugation into Streptomyces]]). Many of the plasmids commonly used for <em>Streptomyces</em> work are '''integrative''': they insert into a specific site on the <em>Streptomyces</em> chromosome using a [[site-specific phage recombinase]].  
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Various plasmids have been developed for use in <em>Streptomyces</em> (most will also contain an origin of replication that functions in <em>Escherichia coli</em>, for cloning, general maintenance, and [[intergeneric conjugation into Streptomyces]]). Many of the plasmids commonly used for <em>Streptomyces</em> work are '''integrative''': they insert into a specific site on the <em>Streptomyces</em> chromosome using a [[site-specific phage recombinase]].  
  
 
The following is an alphabetical list of a number of useful vectors - see also [[Overview of Streptomyces vectors]].  
 
The following is an alphabetical list of a number of useful vectors - see also [[Overview of Streptomyces vectors]].  

Revision as of 15:59, 20 August 2019

Plasmids

Various plasmids have been developed for use in Streptomyces (most will also contain an origin of replication that functions in Escherichia coli, for cloning, general maintenance, and intergeneric conjugation into Streptomyces). Many of the plasmids commonly used for Streptomyces work are integrative: they insert into a specific site on the Streptomyces chromosome using a site-specific phage recombinase.

The following is an alphabetical list of a number of useful vectors - see also Overview of Streptomyces vectors.

Plasmids used in Streptomyces

pCAP03

pCRISPomyces-2

pESAC13

pIJ101

pIJ8660

pIJ10257

pIJ12738

pIJ12742

pKC1132

pMF96

pMS82

pRT802

pSET152

pSS170

Supercos1

pGM9

pGM190


Plasmids used for PCR targeting (Redirect)

Redirect protocol and template information

pIJ773


Plasmids used for heterologous protein expression in E. coli

The following plasmids are frequently used to express proteins heterologously in E. coli, e.g. for two-hybrid analysis or protein purification.

pET28a

Vectors for Bacterial two-hybrid experiments pKT25/pKNT25 | pUT18/pUT18C