Protocols: Difference between revisions
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[[CRISPR/Cas9-mediated genome editing]] | [[CRISPR/Cas9-mediated genome editing]] | ||
[[Chemical mutagenesis]] | |||
[[ChIP Seq]] | |||
[[Codon Optimising Sequences for Heterologous Expression in Streptomyces]] | |||
[[Depletion strains]] | |||
[[General Growth and Maintenance of Streptomyces sp.]] | [[General Growth and Maintenance of Streptomyces sp.]] | ||
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[[Isolating Actinomycetes from the environment]] | [[Isolating Actinomycetes from the environment]] | ||
[[Isolating extracellular protein from Streptomyces]] | |||
[[Lambda-red mediated recombination (PCR-targeting system a.k.a. "Redirect")]] | |||
[[Lambda-red mediated recombination using ssDNA]] | |||
[[Meganuclease I-SceI based system for gene deletions]] | |||
[[Metabolite extractions]] | [[Metabolite extractions]] | ||
[["Quikchange" site-directed mutagenesis]] | |||
[[Random mutagenesis using a mutator strain]] | |||
[[Random mutagenesis using error-prone PCR]] | |||
[[RNA extraction from Actinobacteria]] | |||
[[Salting Out Genomic DNA Extraction Method]] | |||
[[Southern Blotting]] | |||
[[Staining of ''Streptomyces'' spp. Cell Wall and Nucleic Acids| Staining of ''Streptomyces'' spp. Cell Wall and Nucleic Acids]] | |||
[[Suicide vectors]] | |||
[[Transposons]] | |||
[[UV mutagenesis]] | |||
=Protocols organised by category= | =Protocols organised by category= | ||
Revision as of 13:55, 27 September 2019
Alphabetical list of protocols
Characterisation of Actinomycetes
Cloning, Transformation and Expression of Gene Clusters
CRISPR/Cas9-mediated genome editing
Codon Optimising Sequences for Heterologous Expression in Streptomyces
General Growth and Maintenance of Streptomyces sp.
GUS assay (β-Glucuronidase assay)
Isolating Actinomycetes from the environment
Isolating extracellular protein from Streptomyces
Lambda-red mediated recombination (PCR-targeting system a.k.a. "Redirect")
Lambda-red mediated recombination using ssDNA
Meganuclease I-SceI based system for gene deletions
"Quikchange" site-directed mutagenesis
Random mutagenesis using a mutator strain
Random mutagenesis using error-prone PCR
RNA extraction from Actinobacteria
Salting Out Genomic DNA Extraction Method
Staining of Streptomyces spp. Cell Wall and Nucleic Acids
