Protocols: Difference between revisions
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=Protocols organised by category= | =Protocols organised by category= | ||
[[Microbiology]] | ==[[Microbiology]]== | ||
[[Microscopy]] | ==[[Microscopy]]== | ||
[[Mutagenesis]] | ==[[Mutagenesis]]== | ||
[[Natural Products]] | ==[[Natural Products]]== | ||
[[Nucleic Acid Methods]] | ==[[Nucleic Acid Methods]]== | ||
[[Protein Methods]] | ==[[Protein Methods]]== | ||
[[Reporter Gene Assays]] | ==[[Reporter Gene Assays]]== | ||
=Alphabetical list of protocols= | =Alphabetical list of protocols= | ||
Revision as of 13:58, 27 September 2019
Protocols organised by category
Microbiology
Microscopy
Mutagenesis
Natural Products
Nucleic Acid Methods
Protein Methods
Reporter Gene Assays
Alphabetical list of protocols
Characterisation of Actinomycetes
Cloning, Transformation and Expression of Gene Clusters
CRISPR/Cas9-mediated genome editing
Codon Optimising Sequences for Heterologous Expression in Streptomyces
General Growth and Maintenance of Streptomyces sp.
GUS assay (β-Glucuronidase assay)
Isolating Actinomycetes from the environment
Isolating extracellular protein from Streptomyces
Lambda-red mediated recombination (PCR-targeting system a.k.a. "Redirect")
Lambda-red mediated recombination using ssDNA
Meganuclease I-SceI based system for gene deletions
"Quikchange" site-directed mutagenesis
Random mutagenesis using a mutator strain
Random mutagenesis using error-prone PCR
RNA extraction from Actinobacteria
Salting Out Genomic DNA Extraction Method
