Protocols: Difference between revisions
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Revision as of 14:12, 27 September 2019
Protocols organised by category
Biochemistry
- GUS assay (β-Glucuronidase assay)
Microbiology
Microscopy
Mutagenesis
Natural Products
Next Generation Sequencing
Nucleic Acid Methods
Protein Methods
Alphabetical list of protocols
Characterisation of Actinomycetes
Cloning, Transformation and Expression of Gene Clusters
CRISPR/Cas9-mediated genome editing
Codon Optimising Sequences for Heterologous Expression in Streptomyces
General Growth and Maintenance of Streptomyces sp.
GUS assay (β-Glucuronidase assay)
Isolating Actinomycetes from the environment
Isolating extracellular protein from Streptomyces
Lambda-red mediated recombination (PCR-targeting system a.k.a. "Redirect")
Lambda-red mediated recombination using ssDNA
Meganuclease I-SceI based system for gene deletions
"Quikchange" site-directed mutagenesis
Random mutagenesis using a mutator strain
Random mutagenesis using error-prone PCR
RNA extraction from Actinobacteria
Salting Out Genomic DNA Extraction Method
