Protocols: Difference between revisions

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==[[Next Generation Sequencing]]==
==[[Next Generation Sequencing]]==


==[[ChIP Sequencing]]==
*[[ChIP Sequencing]]
==[[ChIP-exo sequencing]]==
*[[ChIP-exo sequencing]]
==[[RNA Sequencing]]==
*[[RNA Sequencing]]
==[[Differential RNA sequencing]]==
*[[Differential RNA sequencing]]


==[[Nucleic Acid Methods]]==
==[[Nucleic Acid Methods]]==

Revision as of 14:18, 27 September 2019

Protocols

Protocols organised by category

Biochemistry

Microbiology

Microscopy

Mutagenesis

Natural Products


Next Generation Sequencing

Nucleic Acid Methods

Protein Methods


Alphabetical list of protocols

Characterisation of Actinomycetes

Cloning, Transformation and Expression of Gene Clusters

CRISPR/Cas9-mediated genome editing

Chemical mutagenesis

ChIP Seq

Codon Optimising Sequences for Heterologous Expression in Streptomyces

Depletion strains

General Growth and Maintenance of Streptomyces sp.

GUS assay (β-Glucuronidase assay)

High GC PCR

Isolating Actinomycetes from the environment

Isolating extracellular protein from Streptomyces

Lambda-red mediated recombination (PCR-targeting system a.k.a. "Redirect")

Lambda-red mediated recombination using ssDNA

Meganuclease I-SceI based system for gene deletions

Metabolite extractions

"Quikchange" site-directed mutagenesis

Random mutagenesis using a mutator strain

Random mutagenesis using error-prone PCR

RNA extraction from Actinobacteria

Salting Out Genomic DNA Extraction Method

Southern Blotting

Staining of Streptomyces spp. Cell Wall and Nucleic Acids

Suicide vectors

Transposons

UV mutagenesis