Protocols: Difference between revisions

From ActinoBase
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*[[Characterisation of Actinomycetes]]
*[[Characterisation of Actinomycetes]]
*[[Exploration | ''Streptomyces'' Exploration]]


*[[General Growth and Maintenance of Streptomyces sp.]]
*[[General Growth and Maintenance of Streptomyces sp.]]

Revision as of 15:41, 10 October 2019

Protocols

Protocols organised by category

Biochemistry

Microbiology

Microscopy

Mutagenesis

Natural Products

Next Generation Sequencing

https://www.nature.com/articles/s41467-018-05265-7/

Nucleic Acid Methods

Protein Methods


Alphabetical list of protocols

Characterisation of Actinomycetes

Cloning, Transformation and Expression of Gene Clusters

CRISPR/Cas9-mediated genome editing

Chemical mutagenesis

ChIP Seq

Codon Optimising Sequences for Heterologous Expression in Streptomyces

Depletion strains

General Growth and Maintenance of Streptomyces sp.

GUS assay (β-Glucuronidase assay)

High GC PCR

Isolating Actinomycetes from the environment

Isolating extracellular protein from Streptomyces

Lambda-red mediated recombination (PCR-targeting system a.k.a. "Redirect")

Lambda-red mediated recombination using ssDNA

Meganuclease I-SceI based system for gene deletions

Metabolite extractions

"Quikchange" site-directed mutagenesis

Random mutagenesis using a mutator strain

Random mutagenesis using error-prone PCR

RNA extraction from Actinobacteria

Salting Out Genomic DNA Extraction Method

Southern Blotting

Staining of Streptomyces spp. Cell Wall and Nucleic Acids

Suicide vectors

Transposons

UV mutagenesis