SMM: Difference between revisions
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=SMM (Supplemented liquid minimal media)= | =SMM (Supplemented liquid minimal media)= | ||
SMM is a supplemented minimal medium suitable for growth of many ''Streptomyces'' sp. | |||
==Preparation== | ==Preparation== | ||
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#With the exception of the trace element solution each solution must be made up and autoclaved separately | #With the exception of the trace element solution each solution must be made up and autoclaved separately | ||
#The solutions should be mixed in the given order and then made up to 1L with sterile distilled water | #The solutions should be mixed in the given order and then made up to 1L with sterile distilled water | ||
==References== | |||
Strauch E, Takano E, Baylts H, Bibb M. The stringent response in ''Streptomyces coelicolor'' A3(2). ''Molecular Microbiology''. 1991;5(2):289-298. |
Revision as of 15:52, 1 August 2019
SMM (Supplemented liquid minimal media)
SMM is a supplemented minimal medium suitable for growth of many Streptomyces sp.
Preparation
Per litre:
- 81.9 mL PEG 6000 (BDH 6.1% w/v in distilled water)
- 2.5 mL Mg2SO4.7H2O - 5mM final
- 10 mL TES bugger (0.25M, pH 7.2) - 25mM final
- 1 mL NaH2PO4 + K2HPO4 (50mM each) - 1mM final
- 2 mL glucose (50% w/v)
- 1 mL Antifoam 289 (Sigma A 5551)(1% w/v)
- 0.1 mL Trace element solution
- 1 mL Difco Casaminoacids (20% w/v)
- 100 mL Total
- 25 ml/L Glycine (0.5% final)
Trace element solution: 0.1 g/L of the following:
- ZnSO4.7H2O
- FeSO4.7H2O
- MnCl2.4H2O
- CaCl2.6H2O
- NaCl
Make fresh every 2-4 weeks and filter-sterilise. Store at 4º
Instructions
- With the exception of the trace element solution each solution must be made up and autoclaved separately
- The solutions should be mixed in the given order and then made up to 1L with sterile distilled water
References
Strauch E, Takano E, Baylts H, Bibb M. The stringent response in Streptomyces coelicolor A3(2). Molecular Microbiology. 1991;5(2):289-298.