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From ActinoBase
Combined display of all available logs of ActinoBase. You can narrow down the view by selecting a log type, the username (case-sensitive), or the affected page (also case-sensitive).
- 12:00, 12 March 2024 User account Annie talk contribs was created by Matt Hutchings talk contribs (New editor)
- 21:09, 22 January 2024 Gilles talk contribs created page CUBIC (Created page with "=CUBIC= CUBC is a cumate-based inducible CRISPR interference (CRISPRi) system that allows efficient and inducible gene knockdown in Streptomyces. Click the link to find out more: CUBIC: A Versatile Cumate-Based Inducible CRISPRi System in Streptomyces pCB-1 PCB-2 ==Organisms== CUBIC has been confirmed to work for the following species: *<em>Streptomyces coelicolor</em> *<em>Streptomyces lividans</em> *<em>Streptomyces venezuelae</em> *<em>Streptomyc...")
- 16:44, 1 November 2023 RTEvans talk contribs moved page RRNA depletion with Streptomyces coelicolor A3(2) specific probes. to RRNA depletion with Streptomyces coelicolor A3(2) specific probes over redirect
- 16:44, 1 November 2023 RTEvans talk contribs deleted redirect RRNA depletion with Streptomyces coelicolor A3(2) specific probes by overwriting (Deleted to make way for move from "RRNA depletion with Streptomyces coelicolor A3(2) specific probes.")
- 16:42, 1 November 2023 RTEvans talk contribs moved page RRNA depletion with Streptomyces coelicolor A3(2) specific probes to RRNA depletion with Streptomyces coelicolor A3(2) specific probes. (Removing capitalised R in rRNA)
- 15:59, 1 November 2023 RTEvans talk contribs uploaded a new version of File:PanelD.png (larger)
- 15:59, 1 November 2023 RTEvans talk contribs uploaded a new version of File:PanelC.png (larger)
- 15:58, 1 November 2023 RTEvans talk contribs uploaded a new version of File:PanelA.png (larger)
- 15:56, 1 November 2023 RTEvans talk contribs uploaded a new version of File:PanelD.png (Larger)
- 15:52, 1 November 2023 RTEvans talk contribs uploaded a new version of File:PanelB.png (Larger)
- 15:51, 1 November 2023 RTEvans talk contribs uploaded a new version of File:PanelA.png (Larger)
- 15:49, 1 November 2023 RTEvans talk contribs uploaded a new version of File:PanelA.png (Larger)
- 15:36, 1 November 2023 RTEvans talk contribs created page File:PanelD.png (Genome mapping of S. coelicolor samples following rRNA depletion using protocol described on this page)
- 15:36, 1 November 2023 RTEvans talk contribs uploaded File:PanelD.png (Genome mapping of S. coelicolor samples following rRNA depletion using protocol described on this page)
- 15:35, 1 November 2023 RTEvans talk contribs created page File:PanelC.png (Genome mapping of S. coelicolor samples following rRNA depletion using Illumina Ribo-zero rRNA Removal Kit (Bacteria))
- 15:35, 1 November 2023 RTEvans talk contribs uploaded File:PanelC.png (Genome mapping of S. coelicolor samples following rRNA depletion using Illumina Ribo-zero rRNA Removal Kit (Bacteria))
- 15:35, 1 November 2023 RTEvans talk contribs created page File:PanelB.png (Genome mapping of S. coelicolor samples following rRNA depletion using NEBNext rRNA Depletion Kit (Bacteria))
- 15:35, 1 November 2023 RTEvans talk contribs uploaded File:PanelB.png (Genome mapping of S. coelicolor samples following rRNA depletion using NEBNext rRNA Depletion Kit (Bacteria))
- 15:33, 1 November 2023 RTEvans talk contribs created page File:PanelA.png (Genome mapping of S. coelicolor samples following rRNA depletion with Qiagen FastSelect 5S/16S/23S kit)
- 15:33, 1 November 2023 RTEvans talk contribs uploaded File:PanelA.png (Genome mapping of S. coelicolor samples following rRNA depletion with Qiagen FastSelect 5S/16S/23S kit)
- 15:26, 1 November 2023 RTEvans talk contribs created page File:RRNA depletion table 1.png (Table 1 - list of abundant non mRNA RNAs in S. coelicolor A3(2))
- 15:26, 1 November 2023 RTEvans talk contribs uploaded File:RRNA depletion table 1.png (Table 1 - list of abundant non mRNA RNAs in S. coelicolor A3(2))
- 15:25, 1 November 2023 RTEvans talk contribs created page RRNA depletion with Streptomyces coelicolor A3(2) specific probes (Created page with "==Organisms== This protocol has been tested with <em>Streptomyces coelicolor</em> A3(2) MT1110 ==rRNA depletion with <em>Streptomyces coelicolor</em> A3(2) specific probes== rRNA depletion using commercial kits targeting generic bacterial sequences is not 100% efficient in the High GC organism S. coelicolor. Depletion using the NEBNext RNA Depletion Core Reagent Set with RNA Sample Purification Beads (https://www.neb.com/en-gb/products/e7870-nebnext-rna-depleti...")
- 11:26, 1 November 2023 User account RTEvans talk contribs was created by MFeeney talk contribs (new user account)
- 07:36, 11 October 2023 User account Gilles talk contribs was created by Matt Hutchings talk contribs
- 17:01, 28 September 2023 User account Mgonza local talk contribs was created automatically
- 16:16, 12 September 2023 Matt Hutchings talk contribs undeleted page CRISPR (5 revisions)
- 15:59, 12 September 2023 Matt Hutchings talk contribs created page MtrA methods are here (Created page with "Som N, Devine R, Chandra G, Martins C, Wilkinson B and Hutchings MI (2023). MtrA regulates the expression of developmental genes, including BldM and WhiI, in Streptomyces vene...")
- 15:57, 12 September 2023 Matt Hutchings talk contribs created page Methods (Created page with "This allows you to publish supplementary methods to support, and cite, in your publications. Email matt.hutchings@jic.ac.uk Som N, Devine R, Chandra G, Martins C, Wilkinson...")
- 07:52, 21 August 2023 Matt Hutchings talk contribs created page VProtocols (Created page with "Below are links to video protocols created by our members: Streptomyces spore preparation by Lorena Fernández-Martínez <Fernandl@edgehill.ac.uk>")
- 09:18, 30 June 2023 User account Tomgreg talk contribs was created by Matt Hutchings talk contribs
- 07:45, 30 June 2023 User account Thomgregory talk contribs was created by Matt Hutchings talk contribs
- 08:18, 29 June 2023 User account Thomgreg talk contribs was created by Matt Hutchings talk contribs and password was sent by email (New editor)
- 13:41, 13 September 2022 User account LabInsubria talk contribs was created by MFeeney talk contribs
- 10:01, 28 July 2022 User account Nmvior talk contribs was created by MFeeney talk contribs
- 15:11, 21 July 2022 User account Fernandl talk contribs was created by MFeeney talk contribs (password reset not working)
- 14:32, 20 July 2022 User account Lorena talk contribs was created by MFeeney talk contribs and password was sent by email
- 12:35, 28 March 2022 Jake Newitt talk contribs created page Wheat Take-all bioassays (Created page with "=Wheat Take-all bioassays= This assay is used to determine whether ''Streptomyces'' strains are able to produce antifungal compounds that inhibit the wheat root pathogen ''Ga...")
- 09:05, 28 March 2022 Jake Newitt talk contribs created page Imaging GFP streptomyces on arabidopsis roots (Created page with "=Imaging GFP streptomyces on ''Arabidopsis'' roots= ''Streptomyces'' strains that contain pIJ8660/PermE* and are able to produce the eGFP protein encoded by it can be visuali...")
- 21:50, 20 March 2022 MFeeney talk contribs created page SV1 transduction (Created page with "=<strong>Phage transduction using SV1</strong>= This protocol has been confirmed to work for the following organisms: *<em>Streptomyces venezuelae</em> with ΦSV1 ===Ph...")
- 16:38, 12 November 2021 User account Nelly talk contribs was created by Matt Hutchings talk contribs (New editor)
- 18:01, 3 November 2021 Sam Prudence talk contribs changed group membership for Agustina Undabarrena from (none) to administrator, interface administrator and bureaucrat
- 18:00, 3 November 2021 User account Agustina Undabarrena talk contribs was created by Sam Prudence talk contribs
- 14:18, 2 November 2021 Damien Gayrard talk contribs created page 1/2 MS+B5 (Created page with "==Preparation== Per Litre: *2.205 MS Salts and Gamborg B5 Vitamins (Duchefa M0231) *1 L ddH2O *5M KOH *Bacto Agar '''Instructions''' #Dissolve 2.2g MS Salts + Gamborg B5 Vit...")
- 11:09, 2 November 2021 User account Damien Gayrard talk contribs was created by Jake Newitt talk contribs
- 12:31, 29 October 2021 Louisa perry jic talk contribs created page 1/2 MS0 (Created page with "==Preparation== Per Litre: *2.205 g g Murashige & Skoog Medium Without Vitamins *1 L tap water *NaOH '''Instructions''' #Dissolve 2.205g Murashige & Skoog in 1L tap water #...")
- 12:24, 29 October 2021 Louisa perry jic talk contribs created page Wheat root exudate harvesting (Created page with "{{DISPLAYTITLE: Wheat root exudate harvesting}} <h1>Wheat root exudates and the recruitment of root microbes</h1> ... <h1>You will need:</h1> Preparation of growth tubes * 5...")
- 14:25, 26 October 2021 Jake Newitt talk contribs created page Wheat seed sterilisation (Created page with "{{DISPLAYTITLE: Wheat seed sterilisation}} =Wheat seed sterilisation protocol= Wheat seeds are typically sterilised before growing in sterile conditions, such is the case for...")
- 14:06, 26 October 2021 Louisa perry jic talk contribs created page MS+B5 (Created page with "==Preparation== Per Litre: *2.25 g g Murashige & Skoog Medium Without Vitamins *0.056 g Gamborg B5 Vitamin Mixture *12 g Agar *1 L tap water '''Instructions''' #Dissolve 2.25...")
- 13:24, 26 October 2021 Louisa perry jic talk contribs created page Growing Marchantia from gemma spores (Created page with "{{DISPLAYTITLE: Growing Marchantia from gemma spores}} ==Marchantia and their lifecycle== Marchantia are a genus of plant in the Bryophyte taxonomic division along with mosse...")
- 12:46, 26 October 2021 User account Louisa perry jic talk contribs was created by Jake Newitt talk contribs (new account)
- 12:44, 26 October 2021 User account Louisa perry talk contribs was created by Jake Newitt talk contribs and password was sent by email (new account)
- 11:45, 26 October 2021 User account Louisa Perry talk contribs was created by Jake Newitt talk contribs and password was sent by email (Creating account for colleague)
- 08:03, 21 October 2021 Matt Hutchings talk contribs created page Plant-Microbe interactions (Created page with "In recent years it has become clear that actinomycetes like Streptomyces species are not simply free-living soil bacteria. They also interact with plant roots and can colonise...")
- 07:53, 21 October 2021 Matt Hutchings talk contribs created page Plants (Created page with "150px|Protocols|link=Protocols =Protocols organised by category= ==Bacteriophage== *Streptomyces Bacteriophage plaque a...")
- 16:23, 20 October 2021 Sam Prudence talk contribs changed group membership for Kate Duncan from (none) to administrator, interface administrator and bureaucrat
- 16:21, 20 October 2021 User account Kate Duncan talk contribs was created by Sam Prudence talk contribs
- 16:02, 20 October 2021 Sam Prudence talk contribs changed group membership for Jake Newitt from (none) to administrator, interface administrator and bureaucrat
- 16:01, 20 October 2021 User account Jake Newitt talk contribs was created by Sam Prudence talk contribs
- 14:59, 20 October 2021 User account Sergey Group talk contribs was created by Sam Prudence talk contribs
- 14:57, 20 October 2021 Sam Prudence talk contribs changed group membership for J Newitt from (none) to administrator, interface administrator and bureaucrat
- 14:53, 20 October 2021 Sam Prudence talk contribs changed group membership for Matt Hutchings from (none) to administrator, interface administrator and bureaucrat
- 13:14, 28 July 2021 User account Matt Hutchings talk contribs was created by Sam Prudence talk contribs
- 16:26, 18 May 2021 H Otani talk contribs created page PCRISPR-dCas9 (Created page with "==References== #Tong Y, Charusanti P, Zhang L, Weber T, Lee SY. CRISPR-Cas9 Based Engineering of Actinomycetal Genomes. ''ACS Synth Biol'' 2015 Sep 18;4(9):1020-9. doi: 10.102...")
- 16:25, 18 May 2021 H Otani talk contribs created page PCRISPR-Cas9 (Created page with "==References== #Tong Y, Charusanti P, Zhang L, Weber T, Lee SY. CRISPR-Cas9 Based Engineering of Actinomycetal Genomes. ''ACS Synth Biol'' 2015 Sep 18;4(9):1020-9. doi: 10.102...")
- 16:23, 18 May 2021 H Otani talk contribs created page PTYM18 (Created page with "==References== #Onaka, H., Taniguchi, S., Ikeda, H., Igarashi, Y., and Furumai, T. (2003). pTOYAMAcos, pTYM18, and pTYM19, actinomycete-''Escherichia coli'' integrating vector...")
- 16:22, 18 May 2021 H Otani talk contribs created page PTYM19 (Created page with "==References== #Onaka, H., Taniguchi, S., Ikeda, H., Igarashi, Y., and Furumai, T. (2003). pTOYAMAcos, pTYM18, and pTYM19, actinomycete-''Escherichia coli'' integrating vector...")
- 16:22, 18 May 2021 H Otani talk contribs created page PTOYAMAcos (Created page with "==References== #Onaka, H., Taniguchi, S., Ikeda, H., Igarashi, Y., and Furumai, T. (2003). pTOYAMAcos, pTYM18, and pTYM19, actinomycete-''Escherichia coli'' integrating vector...")
- 10:01, 26 April 2021 User account S Jarmusch talk contribs was created by Sam Prudence talk contribs
- 11:48, 5 January 2021 User account Parva Zarei talk contribs was created by Sam Prudence talk contribs
- 08:59, 18 August 2020 Alicia talk contribs created page Rhodococcus erythropolis (Created page with "==Organism Page== === ''Rhodococcus erythropolis'' === ===Background=== ===Growth & Maintenance=== GYM =====Sporulation===== Nonsporulating ===Strains=== <em>Rhodo...")
- 12:27, 6 June 2020 Sam Prudence talk contribs created page File:Graphical overview LFM.png
- 12:27, 6 June 2020 Sam Prudence talk contribs uploaded File:Graphical overview LFM.png
- 12:23, 6 June 2020 Sam Prudence talk contribs created page File:LFM plasmid maps.png
- 12:23, 6 June 2020 Sam Prudence talk contribs uploaded File:LFM plasmid maps.png
- 12:07, 6 June 2020 Sam Prudence talk contribs created page Meganuclease I-SceI based system for gene deletions (Created page with "=Gene deletion in actinomycetes using I-SceI meganuclease= This protocol allows the reader to make clean deletions in any actinomycete species using an efficient method to gen...")
- 11:54, 6 June 2020 Sam Prudence talk contribs created page Microbispora (Created page with "=<i>Microbispora</i>= Actinobacteria / Actinobacteria / Streptosporangiales / Streptosporangiaceae / <i>Microbispora</i> <i>Microbispora</i> is a genus of aerobic, Gram-posit...")
- 11:53, 6 June 2020 Sam Prudence talk contribs created page File:Microbispora.png
- 11:53, 6 June 2020 Sam Prudence talk contribs uploaded File:Microbispora.png
- 11:04, 6 May 2020 MFeeney talk contribs created page File:SsDNA recombineering oligos.png (oligonucleotide design for ssDNA recombineering)
- 11:04, 6 May 2020 MFeeney talk contribs uploaded File:SsDNA recombineering oligos.png (oligonucleotide design for ssDNA recombineering)
- 10:16, 6 May 2020 MFeeney talk contribs created page File:SsDNA recombineering protocol.png (figure illustrating two key steps in the ssDNA recombineering protocol)
- 10:16, 6 May 2020 MFeeney talk contribs uploaded File:SsDNA recombineering protocol.png (figure illustrating two key steps in the ssDNA recombineering protocol)
- 10:13, 6 May 2020 MFeeney talk contribs created page File:SsDNA diagnostic pcr.png (description of restriction polymorphism and mismatch PCR approaches to identify the mutant allele generated by ssDNA recombineering)
- 10:13, 6 May 2020 MFeeney talk contribs uploaded File:SsDNA diagnostic pcr.png (description of restriction polymorphism and mismatch PCR approaches to identify the mutant allele generated by ssDNA recombineering)
- 11:07, 5 May 2020 MFeeney talk contribs created page Isolation of phages from environmental samples (Created page with "=<strong>Isolation of phages from environmental samples</strong>= This protocol has been confirmed to work for the following organisms: *<em>Streptomyces lividans</em> s...")
- 10:44, 5 May 2020 MFeeney talk contribs created page Isolation of phages from single plaques (Created page with "=<strong>Isolation of phages from single plaques</strong>= This protocol has been confirmed to work for the following organisms: *<em>Streptomyces coelicolor</em> with...")