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From ActinoBase
Combined display of all available logs of ActinoBase. You can narrow down the view by selecting a log type, the username (case-sensitive), or the affected page (also case-sensitive).
- 12:00, 12 March 2024 User account Annie talk contribs was created by Matt Hutchings talk contribs (New editor)
- 21:09, 22 January 2024 Gilles talk contribs created page CUBIC (Created page with "=CUBIC= CUBC is a cumate-based inducible CRISPR interference (CRISPRi) system that allows efficient and inducible gene knockdown in Streptomyces. Click the link to find out more: CUBIC: A Versatile Cumate-Based Inducible CRISPRi System in Streptomyces pCB-1 PCB-2 ==Organisms== CUBIC has been confirmed to work for the following species: *<em>Streptomyces coelicolor</em> *<em>Streptomyces lividans</em> *<em>Streptomyces venezuelae</em> *<em>Streptomyc...")
- 16:44, 1 November 2023 RTEvans talk contribs deleted redirect RRNA depletion with Streptomyces coelicolor A3(2) specific probes by overwriting (Deleted to make way for move from "RRNA depletion with Streptomyces coelicolor A3(2) specific probes.")
- 16:44, 1 November 2023 RTEvans talk contribs moved page RRNA depletion with Streptomyces coelicolor A3(2) specific probes. to RRNA depletion with Streptomyces coelicolor A3(2) specific probes over redirect
- 16:42, 1 November 2023 RTEvans talk contribs moved page RRNA depletion with Streptomyces coelicolor A3(2) specific probes to RRNA depletion with Streptomyces coelicolor A3(2) specific probes. (Removing capitalised R in rRNA)
- 15:59, 1 November 2023 RTEvans talk contribs uploaded a new version of File:PanelD.png (larger)
- 15:59, 1 November 2023 RTEvans talk contribs uploaded a new version of File:PanelC.png (larger)
- 15:58, 1 November 2023 RTEvans talk contribs uploaded a new version of File:PanelA.png (larger)
- 15:56, 1 November 2023 RTEvans talk contribs uploaded a new version of File:PanelD.png (Larger)
- 15:52, 1 November 2023 RTEvans talk contribs uploaded a new version of File:PanelB.png (Larger)
- 15:51, 1 November 2023 RTEvans talk contribs uploaded a new version of File:PanelA.png (Larger)
- 15:49, 1 November 2023 RTEvans talk contribs uploaded a new version of File:PanelA.png (Larger)
- 15:36, 1 November 2023 RTEvans talk contribs uploaded File:PanelD.png (Genome mapping of S. coelicolor samples following rRNA depletion using protocol described on this page)
- 15:36, 1 November 2023 RTEvans talk contribs created page File:PanelD.png (Genome mapping of S. coelicolor samples following rRNA depletion using protocol described on this page)
- 15:35, 1 November 2023 RTEvans talk contribs uploaded File:PanelC.png (Genome mapping of S. coelicolor samples following rRNA depletion using Illumina Ribo-zero rRNA Removal Kit (Bacteria))
- 15:35, 1 November 2023 RTEvans talk contribs created page File:PanelC.png (Genome mapping of S. coelicolor samples following rRNA depletion using Illumina Ribo-zero rRNA Removal Kit (Bacteria))
- 15:35, 1 November 2023 RTEvans talk contribs uploaded File:PanelB.png (Genome mapping of S. coelicolor samples following rRNA depletion using NEBNext rRNA Depletion Kit (Bacteria))
- 15:35, 1 November 2023 RTEvans talk contribs created page File:PanelB.png (Genome mapping of S. coelicolor samples following rRNA depletion using NEBNext rRNA Depletion Kit (Bacteria))
- 15:33, 1 November 2023 RTEvans talk contribs uploaded File:PanelA.png (Genome mapping of S. coelicolor samples following rRNA depletion with Qiagen FastSelect 5S/16S/23S kit)
- 15:33, 1 November 2023 RTEvans talk contribs created page File:PanelA.png (Genome mapping of S. coelicolor samples following rRNA depletion with Qiagen FastSelect 5S/16S/23S kit)
- 15:26, 1 November 2023 RTEvans talk contribs uploaded File:RRNA depletion table 1.png (Table 1 - list of abundant non mRNA RNAs in S. coelicolor A3(2))
- 15:26, 1 November 2023 RTEvans talk contribs created page File:RRNA depletion table 1.png (Table 1 - list of abundant non mRNA RNAs in S. coelicolor A3(2))
- 15:25, 1 November 2023 RTEvans talk contribs created page RRNA depletion with Streptomyces coelicolor A3(2) specific probes (Created page with "==Organisms== This protocol has been tested with <em>Streptomyces coelicolor</em> A3(2) MT1110 ==rRNA depletion with <em>Streptomyces coelicolor</em> A3(2) specific probes== rRNA depletion using commercial kits targeting generic bacterial sequences is not 100% efficient in the High GC organism S. coelicolor. Depletion using the NEBNext RNA Depletion Core Reagent Set with RNA Sample Purification Beads (https://www.neb.com/en-gb/products/e7870-nebnext-rna-depleti...")
- 11:26, 1 November 2023 User account RTEvans talk contribs was created by MFeeney talk contribs (new user account)
- 07:36, 11 October 2023 User account Gilles talk contribs was created by Matt Hutchings talk contribs
- 17:01, 28 September 2023 User account Mgonza local talk contribs was created automatically
- 16:16, 12 September 2023 Matt Hutchings talk contribs undeleted page CRISPR (5 revisions)
- 15:59, 12 September 2023 Matt Hutchings talk contribs created page MtrA methods are here (Created page with "Som N, Devine R, Chandra G, Martins C, Wilkinson B and Hutchings MI (2023). MtrA regulates the expression of developmental genes, including BldM and WhiI, in Streptomyces vene...")
- 15:57, 12 September 2023 Matt Hutchings talk contribs created page Methods (Created page with "This allows you to publish supplementary methods to support, and cite, in your publications. Email matt.hutchings@jic.ac.uk Som N, Devine R, Chandra G, Martins C, Wilkinson...")
- 07:52, 21 August 2023 Matt Hutchings talk contribs created page VProtocols (Created page with "Below are links to video protocols created by our members: Streptomyces spore preparation by Lorena Fernández-Martínez <Fernandl@edgehill.ac.uk>")
- 09:18, 30 June 2023 User account Tomgreg talk contribs was created by Matt Hutchings talk contribs
- 07:45, 30 June 2023 User account Thomgregory talk contribs was created by Matt Hutchings talk contribs
- 08:18, 29 June 2023 User account Thomgreg talk contribs was created by Matt Hutchings talk contribs and password was sent by email (New editor)
- 13:41, 13 September 2022 User account LabInsubria talk contribs was created by MFeeney talk contribs
- 10:01, 28 July 2022 User account Nmvior talk contribs was created by MFeeney talk contribs
- 15:11, 21 July 2022 User account Fernandl talk contribs was created by MFeeney talk contribs (password reset not working)
- 14:32, 20 July 2022 User account Lorena talk contribs was created by MFeeney talk contribs and password was sent by email
- 12:35, 28 March 2022 Jake Newitt talk contribs created page Wheat Take-all bioassays (Created page with "=Wheat Take-all bioassays= This assay is used to determine whether ''Streptomyces'' strains are able to produce antifungal compounds that inhibit the wheat root pathogen ''Ga...")
- 09:05, 28 March 2022 Jake Newitt talk contribs created page Imaging GFP streptomyces on arabidopsis roots (Created page with "=Imaging GFP streptomyces on ''Arabidopsis'' roots= ''Streptomyces'' strains that contain pIJ8660/PermE* and are able to produce the eGFP protein encoded by it can be visuali...")
- 21:50, 20 March 2022 MFeeney talk contribs created page SV1 transduction (Created page with "=<strong>Phage transduction using SV1</strong>= This protocol has been confirmed to work for the following organisms: *<em>Streptomyces venezuelae</em> with ΦSV1 ===Ph...")
- 16:38, 12 November 2021 User account Nelly talk contribs was created by Matt Hutchings talk contribs (New editor)
- 18:01, 3 November 2021 Sam Prudence talk contribs changed group membership for Agustina Undabarrena from (none) to administrator, interface administrator and bureaucrat
- 18:00, 3 November 2021 User account Agustina Undabarrena talk contribs was created by Sam Prudence talk contribs
- 14:18, 2 November 2021 Damien Gayrard talk contribs created page 1/2 MS+B5 (Created page with "==Preparation== Per Litre: *2.205 MS Salts and Gamborg B5 Vitamins (Duchefa M0231) *1 L ddH2O *5M KOH *Bacto Agar '''Instructions''' #Dissolve 2.2g MS Salts + Gamborg B5 Vit...")
- 11:09, 2 November 2021 User account Damien Gayrard talk contribs was created by Jake Newitt talk contribs
- 12:31, 29 October 2021 Louisa perry jic talk contribs created page 1/2 MS0 (Created page with "==Preparation== Per Litre: *2.205 g g Murashige & Skoog Medium Without Vitamins *1 L tap water *NaOH '''Instructions''' #Dissolve 2.205g Murashige & Skoog in 1L tap water #...")
- 12:24, 29 October 2021 Louisa perry jic talk contribs created page Wheat root exudate harvesting (Created page with "{{DISPLAYTITLE: Wheat root exudate harvesting}} <h1>Wheat root exudates and the recruitment of root microbes</h1> ... <h1>You will need:</h1> Preparation of growth tubes * 5...")
- 14:25, 26 October 2021 Jake Newitt talk contribs created page Wheat seed sterilisation (Created page with "{{DISPLAYTITLE: Wheat seed sterilisation}} =Wheat seed sterilisation protocol= Wheat seeds are typically sterilised before growing in sterile conditions, such is the case for...")
- 14:06, 26 October 2021 Louisa perry jic talk contribs created page MS+B5 (Created page with "==Preparation== Per Litre: *2.25 g g Murashige & Skoog Medium Without Vitamins *0.056 g Gamborg B5 Vitamin Mixture *12 g Agar *1 L tap water '''Instructions''' #Dissolve 2.25...")
- 13:24, 26 October 2021 Louisa perry jic talk contribs created page Growing Marchantia from gemma spores (Created page with "{{DISPLAYTITLE: Growing Marchantia from gemma spores}} ==Marchantia and their lifecycle== Marchantia are a genus of plant in the Bryophyte taxonomic division along with mosse...")
- 12:46, 26 October 2021 User account Louisa perry jic talk contribs was created by Jake Newitt talk contribs (new account)
- 12:44, 26 October 2021 User account Louisa perry talk contribs was created by Jake Newitt talk contribs and password was sent by email (new account)
- 11:45, 26 October 2021 User account Louisa Perry talk contribs was created by Jake Newitt talk contribs and password was sent by email (Creating account for colleague)
- 08:03, 21 October 2021 Matt Hutchings talk contribs created page Plant-Microbe interactions (Created page with "In recent years it has become clear that actinomycetes like Streptomyces species are not simply free-living soil bacteria. They also interact with plant roots and can colonise...")
- 07:53, 21 October 2021 Matt Hutchings talk contribs created page Plants (Created page with "150px|Protocols|link=Protocols =Protocols organised by category= ==Bacteriophage== *Streptomyces Bacteriophage plaque a...")
- 16:23, 20 October 2021 Sam Prudence talk contribs changed group membership for Kate Duncan from (none) to administrator, interface administrator and bureaucrat
- 16:21, 20 October 2021 User account Kate Duncan talk contribs was created by Sam Prudence talk contribs
- 16:02, 20 October 2021 Sam Prudence talk contribs changed group membership for Jake Newitt from (none) to administrator, interface administrator and bureaucrat
- 16:01, 20 October 2021 User account Jake Newitt talk contribs was created by Sam Prudence talk contribs
- 14:59, 20 October 2021 User account Sergey Group talk contribs was created by Sam Prudence talk contribs
- 14:57, 20 October 2021 Sam Prudence talk contribs changed group membership for J Newitt from (none) to administrator, interface administrator and bureaucrat
- 14:53, 20 October 2021 Sam Prudence talk contribs changed group membership for Matt Hutchings from (none) to administrator, interface administrator and bureaucrat
- 13:14, 28 July 2021 User account Matt Hutchings talk contribs was created by Sam Prudence talk contribs
- 16:26, 18 May 2021 H Otani talk contribs created page PCRISPR-dCas9 (Created page with "==References== #Tong Y, Charusanti P, Zhang L, Weber T, Lee SY. CRISPR-Cas9 Based Engineering of Actinomycetal Genomes. ''ACS Synth Biol'' 2015 Sep 18;4(9):1020-9. doi: 10.102...")
- 16:25, 18 May 2021 H Otani talk contribs created page PCRISPR-Cas9 (Created page with "==References== #Tong Y, Charusanti P, Zhang L, Weber T, Lee SY. CRISPR-Cas9 Based Engineering of Actinomycetal Genomes. ''ACS Synth Biol'' 2015 Sep 18;4(9):1020-9. doi: 10.102...")
- 16:23, 18 May 2021 H Otani talk contribs created page PTYM18 (Created page with "==References== #Onaka, H., Taniguchi, S., Ikeda, H., Igarashi, Y., and Furumai, T. (2003). pTOYAMAcos, pTYM18, and pTYM19, actinomycete-''Escherichia coli'' integrating vector...")
- 16:22, 18 May 2021 H Otani talk contribs created page PTYM19 (Created page with "==References== #Onaka, H., Taniguchi, S., Ikeda, H., Igarashi, Y., and Furumai, T. (2003). pTOYAMAcos, pTYM18, and pTYM19, actinomycete-''Escherichia coli'' integrating vector...")
- 16:22, 18 May 2021 H Otani talk contribs created page PTOYAMAcos (Created page with "==References== #Onaka, H., Taniguchi, S., Ikeda, H., Igarashi, Y., and Furumai, T. (2003). pTOYAMAcos, pTYM18, and pTYM19, actinomycete-''Escherichia coli'' integrating vector...")
- 10:01, 26 April 2021 User account S Jarmusch talk contribs was created by Sam Prudence talk contribs
- 11:48, 5 January 2021 User account Parva Zarei talk contribs was created by Sam Prudence talk contribs
- 08:59, 18 August 2020 Alicia talk contribs created page Rhodococcus erythropolis (Created page with "==Organism Page== === ''Rhodococcus erythropolis'' === ===Background=== ===Growth & Maintenance=== GYM =====Sporulation===== Nonsporulating ===Strains=== <em>Rhodo...")
- 12:27, 6 June 2020 Sam Prudence talk contribs uploaded File:Graphical overview LFM.png
- 12:27, 6 June 2020 Sam Prudence talk contribs created page File:Graphical overview LFM.png
- 12:23, 6 June 2020 Sam Prudence talk contribs uploaded File:LFM plasmid maps.png
- 12:23, 6 June 2020 Sam Prudence talk contribs created page File:LFM plasmid maps.png
- 12:07, 6 June 2020 Sam Prudence talk contribs created page Meganuclease I-SceI based system for gene deletions (Created page with "=Gene deletion in actinomycetes using I-SceI meganuclease= This protocol allows the reader to make clean deletions in any actinomycete species using an efficient method to gen...")
- 11:54, 6 June 2020 Sam Prudence talk contribs created page Microbispora (Created page with "=<i>Microbispora</i>= Actinobacteria / Actinobacteria / Streptosporangiales / Streptosporangiaceae / <i>Microbispora</i> <i>Microbispora</i> is a genus of aerobic, Gram-posit...")
- 11:53, 6 June 2020 Sam Prudence talk contribs uploaded File:Microbispora.png
- 11:53, 6 June 2020 Sam Prudence talk contribs created page File:Microbispora.png
- 11:04, 6 May 2020 MFeeney talk contribs uploaded File:SsDNA recombineering oligos.png (oligonucleotide design for ssDNA recombineering)
- 11:04, 6 May 2020 MFeeney talk contribs created page File:SsDNA recombineering oligos.png (oligonucleotide design for ssDNA recombineering)
- 10:16, 6 May 2020 MFeeney talk contribs uploaded File:SsDNA recombineering protocol.png (figure illustrating two key steps in the ssDNA recombineering protocol)
- 10:16, 6 May 2020 MFeeney talk contribs created page File:SsDNA recombineering protocol.png (figure illustrating two key steps in the ssDNA recombineering protocol)
- 10:13, 6 May 2020 MFeeney talk contribs uploaded File:SsDNA diagnostic pcr.png (description of restriction polymorphism and mismatch PCR approaches to identify the mutant allele generated by ssDNA recombineering)
- 10:13, 6 May 2020 MFeeney talk contribs created page File:SsDNA diagnostic pcr.png (description of restriction polymorphism and mismatch PCR approaches to identify the mutant allele generated by ssDNA recombineering)
- 11:07, 5 May 2020 MFeeney talk contribs created page Isolation of phages from environmental samples (Created page with "=<strong>Isolation of phages from environmental samples</strong>= This protocol has been confirmed to work for the following organisms: *<em>Streptomyces lividans</em> s...")
- 10:44, 5 May 2020 MFeeney talk contribs created page Isolation of phages from single plaques (Created page with "=<strong>Isolation of phages from single plaques</strong>= This protocol has been confirmed to work for the following organisms: *<em>Streptomyces coelicolor</em> with...")
- 10:09, 5 May 2020 MFeeney talk contribs created page High titre preparation of phage (Created page with "=<strong>High titre preparation of ''Streptomyces'' phage</strong>= This protocol has been confirmed to work for the following organisms: *<em>Streptomyces coelicolor</e...")
- 09:47, 5 May 2020 MFeeney talk contribs created page File:PhiC31 serial dilution.jpg (Plaque assay: serial dilution of phiC31 on pglW- S. coelicolor)
- 09:47, 5 May 2020 MFeeney talk contribs uploaded File:PhiC31 serial dilution.jpg (Plaque assay: serial dilution of phiC31 on pglW- S. coelicolor)
- 09:02, 5 May 2020 MFeeney talk contribs created page Streptomyces Bacteriophage plaque assay (Created page with "=<strong>Streptomyces bacteriophage plaque assay</strong>= This protocol has been confirmed to work for the following organisms: *<em>Streptomyces coelicolor</em> with...")
- 08:34, 5 May 2020 MFeeney talk contribs uploaded File:PhiC31 plaque assay.jpg (PhiC31 plaque assay on a pglW- strain of S. coelicolor)
- 08:34, 5 May 2020 MFeeney talk contribs created page File:PhiC31 plaque assay.jpg (PhiC31 plaque assay on a pglW- strain of S. coelicolor)
- 08:31, 5 May 2020 MFeeney talk contribs deleted page File:PhiC31 serial dilution.jpg
- 08:31, 5 May 2020 MFeeney talk contribs uploaded File:PhiC31 serial dilution.jpg (PhiC31 plaque assay)
- 08:31, 5 May 2020 MFeeney talk contribs created page File:PhiC31 serial dilution.jpg (PhiC31 plaque assay)
- 22:37, 23 April 2020 LisAlgora talk contribs created page Lambda DNA/HindIII Ladder (Created page with "=Lambda HindIII Marker Preparation= *Lambda (λ) DNA (500 µg/ml): 100µl *10x Reaction Buffer: 45 µl *HindIII or PstI(10U/µl): 5µl *ddWater: 295µl Preparation: #Incubate...")
- 22:35, 23 April 2020 LisAlgora talk contribs created page Lambda DNA/HindIII ladder (Created page with "=Lambda HindIII Marker Preparation= *Lambda (λ) DNA (500 µg/ml): 100µl *10x Reaction Buffer: 45 µl *HindIII or PstI(10U/µl): 5µl *ddWater: 295µl Preparation: #Incubate...")
- 22:23, 23 April 2020 LisAlgora talk contribs created page Solutions for Southern Blotting (Created page with "=Stock Solutions to Prepare in Advance= {| class="wikitable" ! style="font-family:Arial, Helvetica, sans-serif !important;; background-color:rgb(222, 234, 246); color:rgb(0,...")
- 21:55, 23 April 2020 LisAlgora talk contribs created page Consumables for Southern Blotting (Created page with "Roche provides a <b>DIG DNA Labelling and Detection Kit</b> that contains: blocking reagent, Anti-DIG-AP conjugate, Hexanucleotide Mix, DIG DNA Labelling Mix, Klenow Enzyme an...")
- 09:03, 23 April 2020 MFeeney talk contribs moved page Bacteriophage to Streptomyces Bacteriophages without leaving a redirect
- 08:45, 23 April 2020 MFeeney talk contribs created page Bacteriophage (Created page with "=Streptomyces bacteriophages= Prof Paul A Hoskisson, University of Strathclyde. ==Introduction== Bacteriophages (or phages) are amongst the most abundant biological entities...")
- 12:51, 17 April 2020 Sam Prudence talk contribs changed group membership for MHutchings4 from (none) to administrator, interface administrator and bureaucrat
- 12:49, 17 April 2020 User account MHutchings4 talk contribs was created by Sam Prudence talk contribs
- 15:34, 16 April 2020 DaveMark talk contribs created page The ActinoBase e-Seminar (Created page with "=The ActinoBase e-Seminar Series= The ActinoBase e-Seminar Series is an opportunity for both early-career and established researchers in the Actinobacteria community to share...")
- 00:07, 25 March 2020 H Otani talk contribs created page RNA Sequencing (Created page with "#Extract and purify RNA samples according to RNA extraction from Actinobacteria. Add [https://www.thermofisher.com/order/catalog/product/4456740#/4456740 ERCC RNA Spike-In...")
- 23:43, 24 March 2020 H Otani talk contribs created page Natural Products (Created page with "Cloning, Transformation and Expression of Gene Clusters Metabolite extractions Production of microbial volatile organic compounds")
- 21:55, 24 March 2020 H Otani talk contribs created page Protoplast Transformation (Created page with "#Follow the instruction in Protoplasts Formation, Regeneration and Transformation #Add 3.85 ml P buffer to 1 g PEG sterilised. #Add 200 µl PEG-P buffer mixture and a DNA...")
- 17:27, 10 March 2020 Sam Prudence talk contribs uploaded File:Logo4.png
- 17:27, 10 March 2020 Sam Prudence talk contribs created page File:Logo4.png
- 17:17, 10 March 2020 Sam Prudence talk contribs uploaded File:ActinobaseLogo1.gif
- 17:17, 10 March 2020 Sam Prudence talk contribs created page File:ActinobaseLogo1.gif
- 15:09, 28 February 2020 MFeeney talk contribs created page Indicator compounds (Created page with "=Indicator Compounds= ==5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-Gal)== '''Uses''' *Detection of β-galactosidase activity on solid media *Bacterial two-hybrid a...")
- 14:33, 28 February 2020 DaveMark talk contribs uploaded File:PIJ12738 Map.png (Map of PIJ2738)
- 14:33, 28 February 2020 DaveMark talk contribs created page File:PIJ12738 Map.png (Map of PIJ2738)
- 14:06, 28 February 2020 User account Admb talk contribs was created by DaveMark talk contribs
- 15:31, 20 February 2020 User account GenomeBrowser talk contribs was created by Sam Prudence talk contribs
- 15:26, 20 February 2020 Sam Prudence talk contribs moved page Salinospora to Salinispora
- 15:22, 20 February 2020 Sam Prudence talk contribs created page Genome browser (Created page with "<h2><strong>ActinoBase Genome Browser</strong></h2> Click the link below for the ActinoBase MORF Genome Browser, with antiSMASH integration. This tool has been kindly provide...")
- 19:50, 16 February 2020 JoshKlein talk contribs uploaded File:BioArtElvis.PNG (BioArt photo from https://doi.org/10.1371/journal.pbio.1000510.)
- 19:50, 16 February 2020 JoshKlein talk contribs created page File:BioArtElvis.PNG (BioArt photo from https://doi.org/10.1371/journal.pbio.1000510.)
- 19:49, 16 February 2020 JoshKlein talk contribs uploaded File:BioArt.png (A picture of various BioArt plates.)
- 19:49, 16 February 2020 JoshKlein talk contribs created page File:BioArt.png (A picture of various BioArt plates.)
- 15:27, 31 January 2020 ElmiraM talk contribs created page Streptacidiphilus (Created page with "=<i>Streptacidiphilus</i>= Streptacidiphilus (etymology: twisted, acid-loving) are one of the three genera belonging to the <i>Streptomycetaceae<i> family, along with <i>Strep...")
- 15:23, 31 January 2020 MFeeney talk contribs created page Lambda-red mediated recombination using ssDNA (Created page with "=Lambda-red mediated recombination using ssDNA= Lambda-red mediated recombination using ssDNA can be used to introduce precise mutations into the genome of <em>Streptomyces</e...")
- 14:47, 31 January 2020 Jordanmurray talk contribs created page Cultivating Actinobacteria for Microscopic Analysis (Created page with "In order to analyse actinobacteria using light microscopy, methods must take into account the multicellular nature of the test organism. Unlike unicellular organisms, actinoba...")
- 16:31, 29 January 2020 R Devine talk contribs created page ChIP Sequencing (Created page with "==General overview== Chromatin Immunoprecipitation and sequencing can be used to characterise protein-DNA interactions. It works by cross-linking proteins to DNA, shearing t...")
- 16:56, 14 November 2019 Sam Prudence talk contribs deleted page Trace elements (content was: "=Trace Elements= Recipe not added", and the only contributor was "Test" (talk))
- 17:29, 7 November 2019 Sam Prudence talk contribs deleted page CRISPR (content before blanking was: "Hutchings Lab protocol for generating CRISPR/Cas Knockouts using pCRISPomyces-2. Written by Rebecca Devine, Matt Hutchings’ Laboratory, University of East Anglia, UK <strong>Overview (see following pages for more deta...")
- 14:19, 25 October 2019 DaveMark talk contribs created page Isolating Actinobacteria from soil environments (Created page with "Isolating Actinobacteria from soil environments =Equipment & Materials= *Sterile containers to transport the soil *A heat block *Plates of Isolation media *A vortexer *¼ Str...")
- 14:08, 25 October 2019 LeightonP talk contribs created page Using pandoc (add page describing use of pandoc to convert Word documents to MediaWiki format)
- 14:01, 25 October 2019 LeightonP talk contribs created page File:Example word file.png (Screenshot of example Word file, before pandoc conversion)
- 14:01, 25 October 2019 LeightonP talk contribs uploaded File:Example word file.png (Screenshot of example Word file, before pandoc conversion)
- 13:17, 25 October 2019 User account CharlieBegley talk contribs was created by DaveMark talk contribs
- 13:16, 25 October 2019 User account MegJeppesen talk contribs was created by DaveMark talk contribs (New Member)
- 13:15, 25 October 2019 User account MollyKeithBaker talk contribs was created by DaveMark talk contribs (New Member)
- 13:14, 25 October 2019 User account ElmiraM talk contribs was created by DaveMark talk contribs
- 15:16, 17 October 2019 Alicia talk contribs created page Site-specific phage recombinase (Created page with "<strong>Integration sites</strong> You can check the sequence of your organism to see if it contains the appropriate integration site for your chosen plasmid: *ΦBT1, attB...")
- 15:44, 10 October 2019 J Newitt talk contribs created page Links master page (Created page with "=Useful Links= ==Bioinformatics== ===16S rRNA metaprofiling=== [https://greengenes.secondgenome.com Green Genes] 16S rRNA gene database [https://www.arb-silva.de/ SILVA]...")
- 16:25, 30 September 2019 DaveMark talk contribs created page Aminoglycosides (Created page with "=Intro= The aminoglycoside antibiotics are a class of amino containing oligosaccharides, and are notable for their broad-spectrum bacteriocidal activity - including their abil...")
- 14:35, 27 September 2019 LisAlgora talk contribs created page Southern Blotting (Created page with "There are many different protocols for Southern blotting. This approach uses vacuum transfer pf DNA to a nylon membrane, DIG-labelling of the probe and detection by chromogeni...")
- 13:53, 27 September 2019 EmilyAddington talk contribs created page Actinomycetes Morphology and Lifecycle (Created blank page)
- 08:57, 27 September 2019 User account JoshKlein talk contribs was created by MFeeney talk contribs and password was sent by email
- 08:56, 27 September 2019 User account LouCharkoudian talk contribs was created by MFeeney talk contribs and password was sent by email
- 16:01, 26 September 2019 J Newitt talk contribs created page General Growth and Maintenance of Streptomyces sp. (Created page with "=General growth and maintenance of ''Streptomyces'' species= ''Streptomyces'' are multi-nuclear filamentous organisms that undergo several different stages of growth. This se...")
- 15:25, 26 September 2019 Tommclean talk contribs created page Luria (Created page with "=Luria Broth= Known as Luria Broth (or sometimes, mistakenly, as LB or Lennox medium), this rich medium is used routinely for growth of <em>Escherichia coli</em>. There are se...")
- 15:25, 26 September 2019 Sam Prudence talk contribs uploaded File:SCOR2.jpg
- 15:25, 26 September 2019 Sam Prudence talk contribs created page File:SCOR2.jpg
- 15:23, 26 September 2019 Tommclean talk contribs created page Lennox (Created page with "=Lennox Broth= Known as Lennox Broth (or sometimes, mistakenly, as Luria-Bertani medium), this rich medium is used routinely for growth of <em>Escherichia coli</em>. There are...")
- 15:12, 26 September 2019 Tommclean talk contribs created page R5 (Created page with "=R5= ==Preparation== Make up the following solution: *103 g Sucrose *0.25 g K<sub>2</sub>SO<sub>4</sub> *10.12 g MgCl<sub>2</sub>.6H<sub>2</sub>O *10 g Glucose *0.1 g Difco...")
- 15:57, 12 September 2019 Sam Prudence talk contribs created page Isolating extracellular protein from Streptomyces (Created page with " =Isolation of extracellular protein from ''Streptomyces''= This protocol can be used to isolate membrance associated and secreted extracellular proteins from ''Streptomyces''...")
- 09:15, 11 September 2019 Sam Prudence talk contribs created page Test (Created page with "| rowspan="8" valign="top" width="120" | texty wexty ! colspan="2" align="left" | <font face="Arial Black" size="5" style="text-transform: uppercase;"> Heading A </font> | row...")
- 13:20, 4 September 2019 LisAlgora talk contribs created page Trace element solution (Created page with "'''Trace element solution (per liter):''' *40 mg ZnCl<sub>2</sub> *200 mg FeCl<sub>3</sub> . 6H<sub>2</sub>O *10 mg CuCl<sub>2</sub> . 2H<sub>2</sub>O *10 mg MnCl<sub>2</sub>...")
- 13:17, 4 September 2019 LisAlgora talk contribs created page L buffer (Created page with "'''L (lysis) buffer''' (Thompson ''et al.'' 1982) Mix the following sterile solutions: *100 ml Sucrose (10.3%) *10 ml TES buffer (5.73%, adjusted to pH7.2) *1 ml K<sub>2</su...")
- 12:51, 4 September 2019 LisAlgora talk contribs created page P buffer (Created page with "'''P (protoplast) buffer''' (Okanishi ''et al.'', 1974; Hopwood and Wright, 1978) Make up the following basal solution: *103 g Sucrose *0.25 g K<sub>2</sub>SO<sub>4</sub> *2....")
- 09:15, 4 September 2019 User account D Guillen talk contribs was created by Sam Prudence talk contribs
- 14:37, 2 September 2019 User account LeightonP talk contribs was created by MFeeney talk contribs and password was sent by email
- 04:37, 31 August 2019 H Otani talk contribs created page Rhodococcus jostii (Created page with "==Organism Page== === ''Rhodococcus jostii'' === ===Background=== ===Growth & Maintenance=== LB or M9 =====Sporulation===== Nonsporulating ===Strains=== <em>Rh...")
- 00:21, 31 August 2019 H Otani talk contribs created page Rhodococcus (Created page with "Rhodococci are nonsporulating actinomycetes in the family Nocardiaceae along with <em>Nocardia</em>. They are known for their ability to catabolise a wide range of organic...")
- 20:48, 30 August 2019 H Otani talk contribs created page Saccharopolyspora spinosa (Created page with "==Organism Page== === ''Saccharopolyspora spinosa'' === ===Background=== <em>Saccharopolyspora spinosa</em> is known for producing an insecticide, spinosad. Spinosad is a m...")
- 20:37, 30 August 2019 H Otani talk contribs created page YMPD (Created page with "=YMPD (Yeast Meat Peptone Dextrose)= For growth of ''Streptomyces griseus''. ==Preparation== Per litre: *2 g yeast extract *2.2 g meat extract *4 g Bacto peptone *5 g Na...")
- 15:40, 30 August 2019 EmilyAddington talk contribs uploaded a new version of File:Lifecycle.jpeg
- 15:36, 30 August 2019 EmilyAddington talk contribs uploaded File:Lifecycle.jpeg
- 15:36, 30 August 2019 EmilyAddington talk contribs created page File:Lifecycle.jpeg
- 15:28, 30 August 2019 LisAlgora talk contribs created page Protoplasts Formation, Regeneration and Transformation (Created page with "Protoplasts are used for fusion, transformation and transfection. =Protoplasts Preparation= Although this protocol was written for ''Streptomyces lividans'' and ''Str...")
- 15:03, 30 August 2019 ASBirke talk contribs created page Streptomyces clavuligerus (Created page with "=''Streptomyces clavuligerus''= ''Streptomyces clavuligerus'' was originally isolated from a South American soil sample for its ability to produce cephalosporins. Tod...")
- 15:01, 30 August 2019 DaveMark talk contribs created page File:Oligo 3.png
- 15:01, 30 August 2019 DaveMark talk contribs uploaded File:Oligo 3.png
- 14:40, 30 August 2019 MFeeney talk contribs created page Random mutagenesis using a mutator strain (Created page with "=<strong>Random mutagenesis using a mutator strain</strong>= This protocol has been confirmed to work for the following organisms. *<em>Streptomyces coelicolor</em> ==P...")
- 14:32, 30 August 2019 REMcHugh talk contribs created page Conjugation using ET12567/pUZ8002 (Created page with "#Transform ET12467/pUZ8002 with oriT-containing vector, and select for the incoming plasmid only #Inoculate a colony in to 10 ml LB containing Cm, Km and antibiotic used to se...")
- 13:57, 30 August 2019 EmilyAddington talk contribs created page Actinobacteria (Created page with "=Actinobacteria= ==General== ==Taxonomy== ==Ecology== ==Morphology and Lifecycle== ==Actinobacteria genetics== ==Antimicrobial production== ==References== ==Further Reading==")
- 13:53, 30 August 2019 LisAlgora talk contribs created page File:PIJ6902.gif
- 13:53, 30 August 2019 LisAlgora talk contribs uploaded File:PIJ6902.gif
- 13:48, 30 August 2019 LisAlgora talk contribs created page PIJ6902 (Created page with "{{DISPLAYTITLE: pIJ6092}} ==Use== ==Features== *Apramycin resistance cassette *φBT1 integration site & integrase ==History== ==Map== ==Sequence links== ==References=...")
- 13:47, 30 August 2019 User account EmilyAddington talk contribs was created by DaveMark talk contribs
- 13:20, 30 August 2019 User account Aros050 talk contribs was created by MFeeney talk contribs
- 10:52, 30 August 2019 User account D Udwary talk contribs was created by Sam Prudence talk contribs
- 10:48, 30 August 2019 User account H Otani talk contribs was created by Sam Prudence talk contribs
- 15:35, 29 August 2019 Tommclean talk contribs created page Exploration (Created page with "==General overview== <sup>[1]</sup> <strong>Useful host strains:</strong> *<em>Streptomyces venezuelae </em> <sup>[1]</sup> This species is currently the most well character...")
- 15:13, 29 August 2019 Tommclean talk contribs created page Characterisation of Actinomycetes (Created page with "Exploration Production of microbial volatile organic compounds")
- 14:50, 29 August 2019 User account Xtang talk contribs was created by Sam Prudence talk contribs
- 06:27, 29 August 2019 Yvonne Mast talk contribs created page Genome-based taxonomic analysis (Created page with "[https://tygs.dsmz.de/ TYGS] Type (Strain) Genome Server")
- 15:26, 27 August 2019 MFeeney talk contribs created page Teaching and Learning Resources: Antibiotics and AMR (Created page with "=Teaching and Learning Resources: Antibiotics and AMR= This section is our spotlight on teaching resources aimed for teaching about antibiotics and antimicrobial resistance at...")
- 11:14, 26 August 2019 Sam Prudence talk contribs protected Contribution Guidelines [Edit=Allow only administrators] (indefinite) [Move=Allow only administrators] (indefinite) (hist)
- 16:01, 20 August 2019 MFeeney talk contribs created page Mutagenesis (Created page with "A number of different methods have been developed for random and directed mutagenesis in actinobacteria (most notably for <em>Streptomyces coelicolor</em>). Many involve an in...")
- 16:25, 15 August 2019 S Worsley talk contribs created page RNA extraction from Actinobacteria (Created page with " =UNFINISHED PAGE: material is still being added= =Summary= This protocol for RNA extraction can be used to isolate large yields of high quality RNA from actinobacterial strai...")
- 15:12, 15 August 2019 User account S Worsley talk contribs was created by Sam Prudence talk contribs
- 10:34, 15 August 2019 Sam Prudence talk contribs created page File:Microsoc logo.png
- 10:34, 15 August 2019 Sam Prudence talk contribs uploaded File:Microsoc logo.png
- 14:44, 14 August 2019 J Newitt talk contribs created page Isolating Streptomyces from plant roots (Created page with "{{DISPLAYTITLE: Isolating ''Streptomyces'' from plant roots}}")
- 14:39, 14 August 2019 J Newitt talk contribs created page Isolating Actinomycetes from the environment (Created page with "Isolating ''Streptomyces'' from plant roots")
- 13:56, 13 August 2019 Alicia talk contribs uploaded a new version of File:PKC1132 Map.png
- 13:55, 13 August 2019 Alicia talk contribs uploaded File:PKC1132 Map.png
- 13:55, 13 August 2019 Alicia talk contribs created page File:PKC1132 Map.png
- 10:11, 13 August 2019 Andyxmas talk contribs created page Map (Created page with "<html> <iframe src="https://www.google.co.uk/maps/d/u/0/embed?mid=14bRNmsDo2vIXP_I8CVOgW4DJGB7ewkuf" width="640" height="480"></iframe> </html>")
- 09:50, 13 August 2019 Sam Prudence talk contribs deleted page Salting Out Genomic DNA Extraction Method (Deleted to make way for move from "Salting Out DNA Extraction Method")
- 09:50, 13 August 2019 Sam Prudence talk contribs moved page Salting Out DNA Extraction Method to Salting Out Genomic DNA Extraction Method
- 09:49, 13 August 2019 Sam Prudence talk contribs moved page Salting out method to Salting Out Genomic DNA Extraction Method
- 09:48, 13 August 2019 Sam Prudence talk contribs moved page Salting out method to Salting Out DNA Extraction Method
- 19:47, 12 August 2019 J Newitt talk contribs created page Salting out method (Created page with "=Isolation of high molecular weight (HMW) genomic DNA by salting out= The salting out method is used to isolate high yields of high molecular weight DNA, usually for applicat...")
- 08:22, 12 August 2019 Sam Prudence talk contribs moved page PCRISPomyces2 to PCRISPomyces-2
- 09:32, 9 August 2019 Alicia talk contribs uploaded File:PIJ10257 Map2.png
- 09:32, 9 August 2019 Alicia talk contribs created page File:PIJ10257 Map2.png
- 19:08, 8 August 2019 DaveMark talk contribs created page Contribution Guidelines (Created page with "==Intro==")
- 17:17, 8 August 2019 Alicia talk contribs uploaded File:PIJ10257 Map.png
- 17:17, 8 August 2019 Alicia talk contribs created page File:PIJ10257 Map.png
- 16:52, 8 August 2019 Sam Prudence talk contribs uploaded File:PCRISPomyces-2 map.png
- 16:52, 8 August 2019 Sam Prudence talk contribs created page File:PCRISPomyces-2 map.png
- 16:45, 8 August 2019 Sam Prudence talk contribs created page File:PCAP03 map.png
- 16:45, 8 August 2019 Sam Prudence talk contribs uploaded File:PCAP03 map.png
- 16:34, 8 August 2019 Sam Prudence talk contribs moved page PKT25 to PKT25/pKNT25
- 16:32, 8 August 2019 Sam Prudence talk contribs moved page PUT18 to PUT18/pUT18C
- 15:25, 8 August 2019 Sam Prudence talk contribs created page File:PRT802 Map.png
- 15:25, 8 August 2019 Sam Prudence talk contribs uploaded File:PRT802 Map.png
- 19:33, 3 August 2019 Sam Prudence talk contribs protected Test page [Create=Allow only administrators] (indefinite)
- 09:34, 3 August 2019 HeeeenaJ talk contribs created page ChIP Seq (Created page with "Buffers:- IP Buffer 50 mM HEPES pH to 7.5 with KOH 150 mM NaCl 1 mM EDTA 1 % Triton X-100 0.1 % Sodium deoxycholate 0.1 % SDS 5X Elution Buffer TE pH 7.6 5% SDS IP Buffer +...")
- 20:14, 2 August 2019 HeeeenaJ talk contribs created page PIJ12742 (Background)
- 12:45, 2 August 2019 Tommclean talk contribs created page Recruitment (Created page with "=Recruitment= Please feel free to post information and links to job opportunities etc below: *Please also remember to remove the links once filled.")
- 12:03, 2 August 2019 Matth talk contribs created page Groups (Created page with "Alphabetical list of research groups working on Streptomyces and closely related bacteria:")
- 10:35, 2 August 2019 User account R Devine talk contribs was created by Sam Prudence talk contribs
- 08:53, 2 August 2019 Sam Prudence talk contribs moved page Cloning and Expression of Gene Clusters to Cloning, Transformation and Expression of Gene Clusters
- 18:05, 1 August 2019 Sam Prudence talk contribs created page Tri-parental mating (Created page with "=<strong>Tri-parental Mating</strong>= ===Why Tri-Parental Mating?=== Tri-parental mating is useful for conjugating plasmids containing a kanamycin resistane gene as a selecta...")
- 15:57, 1 August 2019 Tommclean talk contribs created page SMMS (Created page with "=SMMS (Supplemented liquid minimal media, solid)= SMM is a solid supplemented minimal medium suitable for growth of many ''Streptomyces'' sp. ==Preparation== Per litre: *81.9...")
- 15:56, 1 August 2019 Jacob Hamilton talk contribs created page SPY (Created page with "=Salt Peptone Yeast Agar (SPY)= Salt Peptone Yeast agar is a metal-rich media used to stimulate the activation of cryptic biosynthetic gene clusters as part of antibiotic dis...")
- 15:55, 1 August 2019 Lucasbalis talk contribs created page Protein Methods (Created page with "Codon Optimising Sequences for Heterologous Expression in Streptomyces")
- 15:38, 1 August 2019 Jacob Hamilton talk contribs moved page Potato Dextrose Agar PDA to PDA (Rename)
- 15:36, 1 August 2019 Jacob Hamilton talk contribs moved page PGA to Potato Dextrose Agar PDA (Renaming from glucose to dextrose to maintain consistency)
- 15:33, 1 August 2019 Jacob Hamilton talk contribs created page PGA (Created page with "=Potato Dextrose Agar (PDA)= Potato Dextrose Agar is primarly used for the growth of the opportunistic fungal pathogen ''Scedosporium prolificans''. Thus, it is mostly used...")
- 15:30, 1 August 2019 Tommclean talk contribs created page SMM (Created page with "=SMM (Supplemented liquid minimal media)= Strauch et al., 1991<sup>1</sup> ==Preparation== Per litre: *81.9 mL PEG 6000 (BDH 6.1% w/v in distilled water) *2.5 mL Mg<sub>2</su...")
- 15:24, 1 August 2019 MFeeney talk contribs created page Overview of Streptomyces vectors (Created page with "150px|Plasmids|link=Plasmids =Commonly Used Streptomyces Vectors= This page describes the characteristics of some of the most co...")
- 15:21, 1 August 2019 Alicia talk contribs created page Streptomyces coelicolor A3(2) (Created page with "==Organism Page== === ''Streptomyces coelicolor A3(2)'' === ===Background=== ===Growth & Maintenance=== =====Sporulation===== *Sporulation can be achieved after 3-5 days gr...")
- 11:00, 1 August 2019 MFeeney talk contribs created page HT (Created page with "=Hickey-Tresner agar (HT)= This medium was developed by Hickey and Tresner<sup>[1]</sup> for the growth of some ''Streptomyces'' sp. that did not sporulate well on Bennett's...")
- 13:39, 31 July 2019 MFeeney talk contribs created page Antibiotic stocks and working concentrations (Created page with "{| class="wikitable" ! style="font-weight:bold; background-color:#c0c0c0;" | Antibiotic ! style="font-weight:bold; background-color:#c0c0c0;" | Stock [mg/ml] ! style="font-wei...")
- 13:29, 31 July 2019 User account Paulhoskisson talk contribs was created by MFeeney talk contribs and password was sent by email
- 11:06, 31 July 2019 MFeeney talk contribs created page CM (Created page with "=CM (Complete Medium)= ==Preparation== Per litre: *5g K<sub>2</sub>HPO<sub>4</sub> *0.5g NaCl *0.5g MgSO<sub>4</sub>*7H<sub>2</sub>O *2g Difco Bacto-peptone *1.5g Difco Casa...")
- 08:21, 30 July 2019 MFeeney talk contribs created page IMA (Created page with "=IMA(Instant Mash Agar)= IMA is sometimes also called [https://en.wikipedia.org/wiki/Smash_(instant_mashed_potato) Smash] (after the brand name of the instant potato commonly...")
- 20:47, 29 July 2019 User account HeeeenaJ talk contribs was created by MFeeney talk contribs and password was sent by email
- 10:59, 29 July 2019 CraigAllan talk contribs created page Streptomyces griseus (Created page with "==Organism Page== === ''Streptomyces griseus'' === ===Background=== ===Growth & Maintenance=== =====Sporulation===== ===Strains=== ====Superhosts==== ===Links=== =====Ge...")
- 10:47, 29 July 2019 ActinoCommittee talk contribs changed group membership for CraigAllan from administrator to administrator, interface administrator and bureaucrat
- 10:46, 29 July 2019 ActinoCommittee talk contribs changed group membership for CraigAllan from (none) to administrator
- 10:46, 29 July 2019 User account CraigAllan talk contribs was created by ActinoCommittee talk contribs (Actino Committee)
- 10:07, 29 July 2019 MFeeney talk contribs created page NMMP (Created page with "=Minimal liquid medium (NMMP)= NMMP medium is used to obtain dispersed growth of ''Streptomyces'' sp. with specific carbon sources. ==Preparation== Make up the following sol...")
- 15:12, 28 July 2019 DaveMark talk contribs created page ISP7 (Created page with "=ISP 7= ==Preparation== *15 g Glycerol *0.5 g L-tyrosine *1 g L-asparagine *0.5 g K<sub>2</sub>HPO<sub>4</sub> *0.5 g MgSO<sub>4</sub> x 7H<sub>2</sub>O *0.5 g NaCl *0.01 g...")
- 13:24, 28 July 2019 DaveMark talk contribs created page ISP6 (Created page with "=ISP6= ==Preparation== *36 g Bacto-Peptone Iron Agar (Difco) **15 g Bacto-Peptone **5 g Proteose-peptone ** 0.5 g Ferric ammonium citrate ** 1g K<sub>2</sub>2HPO<sub>4</sub...")
- 12:28, 28 July 2019 DaveMark talk contribs uploaded File:ISP 6 well.png (6-well plate with ISPs 2-7)
- 12:28, 28 July 2019 DaveMark talk contribs created page File:ISP 6 well.png (6-well plate with ISPs 2-7)
- 17:16, 26 July 2019 LisAlgora talk contribs created page R2YE (Created page with "=R2YE= R2YE medium is a variation of R2 containing yeast extract. ==Preparation== Make up the following solution: *103 g Sucrose *0.25 g K<sub>2</sub>SO<sub>4</sub> *10.12 g...")
- 16:01, 26 July 2019 LisAlgora talk contribs created page R2 (Created page with "=R2= ==Preparation== #Make up the following solution: *103 g Sucrose *0.25 g K<sub>2</sub>SO<sub>4</sub> *10.12 g MgCl<sub>2</sub>.6H<sub>2</sub>O *10 g Glucose *0.1 g Difc...")
- 15:57, 26 July 2019 Laia talk contribs deleted redirect Actinobacteria isolation from marine environments by overwriting (Deleted to make way for move from "Category:Actinobacteria isolation from marine environments")
- 15:57, 26 July 2019 Laia talk contribs moved page Category:Actinobacteria isolation from marine environments to Actinobacteria isolation from marine environments over redirect
- 15:55, 26 July 2019 Laia talk contribs moved page Actinobacteria isolation from marine environments to Category:Actinobacteria isolation from marine environments
- 15:52, 26 July 2019 MFeeney talk contribs created page PUT18C (Created page with "{{DISPLAYTITLE: pUT18C}} =pUT18C= ==Background== ==Construction== ==Uses== ==Characteristics== ==Map and Sequence== ==References==")
- 15:51, 26 July 2019 MFeeney talk contribs created page PUT18 (Created page with "{{DISPLAYTITLE: pUT18}} =pUT18= ==Background== ==Construction== ==Uses== ==Characteristics== ==Map and Sequence== ==References==")
- 15:51, 26 July 2019 MFeeney talk contribs created page PKT25 (Created page with "{{DISPLAYTITLE: pKT25}} =pKT25= ==Background== ==Construction== ==Uses== ==Characteristics== ==Map and Sequence== ==References==")
- 15:50, 26 July 2019 MFeeney talk contribs created page PKNT25 (Created page with "{{DISPLAYTITLE: pKNT25}} =pKNT25= ==Background== ==Construction== ==Uses== ==Characteristics== ==Map and Sequence== ==References==")
- 15:50, 26 July 2019 Jordanmurray talk contribs created page Media (Redirected page to Media Recipes) Tag: New redirect
- 15:50, 26 July 2019 MFeeney talk contribs created page PET28a (Created page with "{{DISPLAYTITLE: pET28a}} =pET28a= ==Background== ==Construction== ==Uses== ==Characteristics== ==Map and Sequence== ==References==")
- 15:49, 26 July 2019 MFeeney talk contribs created page PIJ773 (Created page with "{{DISPLAYTITLE: pIJ773}} =pIJ773= ==Background== ==Construction== ==Uses== ==Characteristics== ==Map and Sequence== ==References==")
- 15:49, 26 July 2019 MFeeney talk contribs created page PSS170 (Created page with "{{DISPLAYTITLE: pSS170}} =pSS170= ==Background== ==Construction== ==Uses== ==Characteristics== ==Map and Sequence== ==References==")
- 15:48, 26 July 2019 MFeeney talk contribs created page PRT802 (Created page with "{{DISPLAYTITLE: pRT802}} =pRT802= ==Background== ==Construction== ==Uses== ==Characteristics== ==Map and Sequence== ==References==")
- 15:47, 26 July 2019 MFeeney talk contribs created page PMF96 (Created page with "{{DISPLAYTITLE: pMF96}} =pMF96= ==Background== ==Construction== ==Uses== ==Characteristics== ==Map and Sequence== ==References==")
- 15:47, 26 July 2019 MFeeney talk contribs created page PKC1132 (Created page with "{{DISPLAYTITLE: pKC1132}} =pKC1132= ==Background== ==Construction== ==Uses== ==Characteristics== ==Map and Sequence== ==References==")
- 15:46, 26 July 2019 MFeeney talk contribs created page PIJ12738 (Created page with "{{DISPLAYTITLE: pIJ12738}} =pIJ12738= ==Background== ==Construction== ==Uses== ==Characteristics== ==Map and Sequence== ==References==")
- 15:45, 26 July 2019 MFeeney talk contribs created page PIJ8660 (Created page with "{{DISPLAYTITLE: {{DISPLAYTITLE: pIJ10257}} =pIJ8660= ==Background== ==Construction== ==Uses== ==Characteristics== ==Map and Sequence== ==References==")
- 15:43, 26 July 2019 ActinoCommittee talk contribs created page Metabolite extractions (Protocols to extract metabolites from Actinobacteria)
- 15:38, 26 July 2019 MFeeney talk contribs created page PIJ733 (Created page with "{{DISPLAYTITLE: pIJ773}} =pIJ773= ==Background== ==Construction== ==Uses== ==Characteristics== ==Map and Sequence== ==References==")
- 15:37, 26 July 2019 DaveMark talk contribs created page ISP5 (Created page with "=ISP 5= ==Preparation== *1 g L-asparagine (anhydrous) *10 g Glycerol *1 g K<sub>2</sub>HPO<sub>4</sub> *1 L dH<sub>2</sub>O *1 mL Trace salts solution **0.1 g FeSO<sub>4</s...")
- 15:27, 26 July 2019 REMcHugh talk contribs uploaded File:Sgoldwiki.png
- 15:27, 26 July 2019 REMcHugh talk contribs created page File:Sgoldwiki.png
- 15:22, 26 July 2019 DaveMark talk contribs created page ISP4 (Created page with "=ISP 4= International <i>Streptomyces</i> Project medium 4, also named inorganic salts-starch agar ==Preparation== *10 g Soluble Starch *1 g MgSO<sub>4</sub> x 7H<sub>2</sub>...")
- 15:19, 26 July 2019 REMcHugh talk contribs uploaded File:Sgoldiniensis.png
- 15:19, 26 July 2019 REMcHugh talk contribs created page File:Sgoldiniensis.png
- 15:15, 26 July 2019 MFeeney talk contribs created page PIJ10257 (Created page with "{{DISPLAYTITLE: pIJ10257}} =pIJ10257= ==Background== ==Construction== ==Uses== ==Characteristics== ==Map and Sequence== ==References==")
- 15:11, 26 July 2019 REMcHugh talk contribs uploaded File:Streptomyces goldiniensis.png
- 15:11, 26 July 2019 REMcHugh talk contribs created page File:Streptomyces goldiniensis.png
- 15:09, 26 July 2019 ASBirke talk contribs created page MC agar (Created page with "=MC agar= This media is used to screen Streptomyces strains for extracellular protease production and the resulting degradation of the milk powder components on the plate, wh...")
- 15:09, 26 July 2019 Jordanmurray talk contribs created page Staining of ''Streptomyces'' spp. Cell Wall and Nucleic Acids (Redirected page to Staining of Streptomyces spp. Cell Wall and Nucleic Acids) Tag: New redirect
- 15:09, 26 July 2019 User account LRooney talk contribs was created by MFeeney talk contribs (Additional user)
- 15:04, 26 July 2019 MFeeney talk contribs created page Supercos1 (Created page with " =SuperCos1= ==Background== ==Construction== ==Uses== ==Characteristics== ==Map and Sequence== ==References==")
- 14:59, 26 July 2019 MFeeney talk contribs created page PESAC13 (Created page with "=pESAC13= ==Background== ==Construction== ==Uses== ==Characteristics== ==Map and Sequence== ==References==")
- 14:52, 26 July 2019 MFeeney talk contribs created page SC (Created page with "=Starch Casein Agar= ==Preparation== Per Litre: *10 g soluble starch *2g K<sub>2</sub>HPO<sub>4</sub> *2g KNO<sub>3</sub> *0.3g casein *0.05g MgSO<sub>4</sub>*7H<sub>2</sub>O...")
- 14:46, 26 July 2019 LisAlgora talk contribs created page SNA (Created page with "=SNA= Soft Nutrient Agar (SNA) ==Preparation== Per Litre: *4g Difco Nutrient Broth Powder *5g Agar *1L dH<sub>2</sub>O '''Instructions''' #Add 0.8g of DNB powder into eac...")
- 14:41, 26 July 2019 MFeeney talk contribs created page OT (Created page with "=OT(oligotrophic agar)= Oligotrophic agar is an extremely nutritionally-poor medium useful for isolating slow-growing actinomyces e.g. from soil. ==Preparation== Per litre:...")
- 14:36, 26 July 2019 REMcHugh talk contribs created page Streptomyces goldiniensis (Created page with "=<em>Streptomyces goldiniensis </em>= <em>Streptomyces goldiniensis </em>is a Gram-positive soil dwelling bacterium that was first isolated from the island of Bermuda in 1969...")
- 14:27, 26 July 2019 MFeeney talk contribs created page HV (Created page with "=HV= ==Preparation== Per Litre: *1g humic acid *1.7g KCl *0.5g Na<sub>2</sub>HPO<sub>4</sub> *0.5g MgSO<sub>4</sub> *0.02g CaCO<sub>3</sub> *0.01g FeSO<sub>4</sub> *1mL VB...")
- 14:24, 26 July 2019 LisAlgora talk contribs created page 2X YT (Created page with "=2x YT= ==Preparation== Per litre: *16 g Tryptone *10 g Yeast Extract *5 g NaCl *1 L dH<sub>2</sub>O '''Instructions''' #Dissolve constituents in 1L dH<sub>2</sub>O #Ste...")
- 14:18, 26 July 2019 DaveMark talk contribs created page ISP3 (Created page with "=ISP 3= International Streptomyces Project media 3 (ISP 3) is a rich general use media. Alternatively called oatmeal agar. ==Preparation== *20 g White Oats *18 g Agar *1 mL...")
- 14:18, 26 July 2019 Jordanmurray talk contribs created page Staining of Streptomyces spp. Cell Wall and Nucleic Acids (Created page with "The following staining technique is a useful method for visualising the actively growing regions of the cell wall, along with the nucleic acids within the cell. The method was...")
- 14:08, 26 July 2019 ActinoCommittee talk contribs created page Emerson medium (Created page with "=Emerson medium= Emerson medium is a rich medium formulated by Emerson et al (1) to isolate mould and Actinobacteria. It is composed of beef extract, yeast extract, peptic di...")
- 13:54, 26 July 2019 User account Jacob Hamilton talk contribs was created by Sam Prudence talk contribs
- 13:51, 26 July 2019 User account ASBirke talk contribs was created by MFeeney talk contribs
- 13:48, 26 July 2019 DaveMark talk contribs created page GYM (Created page with "=Glucose Yeast-Extract Malt-Extract Media= ==Preparation== Per litre: *4 g Glucose *4 g Yeast Extract *10 g Malt Extract *CaCo<sub>3</sub> *12 g Agar *1 L dH<sub>2</sub>O...")
- 13:47, 26 July 2019 User account REMcHugh talk contribs was created by MFeeney talk contribs
- 13:36, 26 July 2019 ActinoCommittee talk contribs moved page Environmental isolation to Actinobacteria isolation from marine environments
- 13:31, 26 July 2019 Jordanmurray talk contribs created page Microscopy (Created page with "=Microscopy of Actinobacteria= ==<em>Streptomyces</em>==")
- 13:25, 26 July 2019 User account RMcHugh talk contribs was created by MFeeney talk contribs
- 13:21, 26 July 2019 ActinoCommittee talk contribs created page Environmental isolation (Protocols to isolate Actinobacteria from different environments)
- 13:18, 26 July 2019 User account LisAlgora talk contribs was created by MFeeney talk contribs
- 13:17, 26 July 2019 User account Jordanmurray talk contribs was created by MFeeney talk contribs
- 13:00, 26 July 2019 User account MunnJ90 talk contribs was created by MFeeney talk contribs
- 11:48, 26 July 2019 MFeeney talk contribs created page Teaching and learning (Created page with "This is the hub for #ActinoBase teaching and learning resources: educational content, outreach, and more! We welcome contributions from the community, so please Contact us...")
- 16:06, 25 July 2019 User account Yvonne Mast talk contribs was created by Sam Prudence talk contribs
- 11:15, 24 July 2019 Alicia talk contribs created page Heterologous expression of gene clusters (Created page with "Gene clusters that have been cloned from their native host can be expressed heterologously in a different host organism. This can help improve production of known products, or...")
- 10:50, 24 July 2019 Alicia talk contribs created page PCAP03 (Created page with " The [https://pubs.acs.org/doi/10.1021/acschembio.5b00658 pCAP03 paper]")
- 08:51, 22 July 2019 Matth talk contribs created page CRISPR (Created page with "Hutchings Lab protocol for generating CRISPR/Cas Knockouts using pCRISPomyces-2. Written by Rebecca Devine, Matt Hutchings’ Laboratory, University of East Anglia, UK <em>O...")
- 20:10, 18 July 2019 Matth talk contribs created page CRISPR/Cas9-mediated genome editing (Created page with "pCRISPomyces2")
- 19:43, 17 July 2019 Matth talk contribs created page PSET152 (Created page with "File:pSET152.png")
- 19:43, 17 July 2019 Matth talk contribs uploaded File:PSET152.png
- 19:43, 17 July 2019 Matth talk contribs created page File:PSET152.png
- 19:40, 17 July 2019 Matth talk contribs uploaded File:PMS82.png
- 19:40, 17 July 2019 Matth talk contribs created page File:PMS82.png
- 19:39, 17 July 2019 Matth talk contribs created page PMS82 (Created page with "Integrating vector derived from the φBT1 attP-int locus and carrying the hygromycin resistance cassette. Described [https://jb.asm.org/content/185/17/5320.long here]")
- 19:27, 17 July 2019 Matth talk contribs created page PCRISPomyces2 (Created page with "file:CRISPR.png")
- 19:27, 17 July 2019 Matth talk contribs uploaded File:CRISPR.png
- 19:27, 17 July 2019 Matth talk contribs created page File:CRISPR.png
- 09:35, 5 July 2019 Matth talk contribs deleted page File:Timeline.png (Deleted old revision 20190705093521!Timeline.png)
- 09:35, 5 July 2019 Matth talk contribs uploaded a new version of File:Timeline.png
- 09:33, 5 July 2019 Matth talk contribs deleted page File:Timeline.png (Deleted old revision 20190705085928!Timeline.png)
- 09:33, 5 July 2019 Matth talk contribs deleted page File:Timeline.png (Deleted old revision 20190705093317!Timeline.png)
- 09:33, 5 July 2019 Matth talk contribs uploaded a new version of File:Timeline.png
- 08:59, 5 July 2019 Matth talk contribs uploaded a new version of File:Timeline.png
- 08:58, 5 July 2019 Matth talk contribs uploaded File:Timeline.png
- 08:58, 5 July 2019 Matth talk contribs created page File:Timeline.png
- 08:52, 5 July 2019 Matth talk contribs created page File:Antibiotics.png
- 08:52, 5 July 2019 Matth talk contribs uploaded File:Antibiotics.png
- 08:52, 5 July 2019 Matth talk contribs created page Antibiotics (Created page with "The filamentous actinomycetes make around two thirds of all known natural product antibiotics and about half of the antibiotics used clinically.")
- 16:04, 4 July 2019 J Newitt talk contribs created page ISP2 (Created page with "=International Streptomyces Project-2 (ISP-2)= ISP-2 is a rich medium that is routinely used for cultivating Streptmyces lydicus along with various other plant root associate...")
- 15:50, 4 July 2019 Tommclean talk contribs created page YP (Created page with "See YPD")
- 15:42, 4 July 2019 Tommclean talk contribs created page YPD (Created page with "=Yeast Peptone Dextrose= Yeast Peptone Dextrose (YPD) medium is composed of Yeast Peptone medium (YP) with the addition of dextrose (D-glucose). YPD is a general medium used...")
- 15:25, 4 July 2019 Test talk contribs created page Nucleic Acid Methods (Created page with "ChIP Seq")
- 13:07, 4 July 2019 Matth talk contribs uploaded File:Ps.png
- 13:07, 4 July 2019 Matth talk contribs created page File:Ps.png
- 12:59, 4 July 2019 Matth talk contribs uploaded File:Pseudonocardia.png (Colonies of two different ant-associated Pseudonocardia strains growing on SFM agar)
- 12:59, 4 July 2019 Matth talk contribs created page File:Pseudonocardia.png (Colonies of two different ant-associated Pseudonocardia strains growing on SFM agar)
- 12:55, 4 July 2019 Matth talk contribs uploaded File:Streptomyces.png
- 12:55, 4 July 2019 Matth talk contribs created page File:Streptomyces.png
- 12:52, 4 July 2019 Matth talk contribs created page File:Streptomyces.gif
- 12:52, 4 July 2019 Matth talk contribs uploaded File:Streptomyces.gif
- 12:47, 4 July 2019 Matth talk contribs created page Micromonospora (Created page with "In the family <em>[https://en.wikipedia.org/wiki/Micromonosporaceae Micromonosporaceae]</em>, best known for making [https://en.wikipedia.org/wiki/Gentamicin gentamicin] which...")
- 12:40, 4 July 2019 Matth talk contribs created page Salinospora (Created page with "<Salinospora</em> is a genus of marine actinomycetes in the family <em>Micromonosporaceae</em. best known for making [salinosporamide a salinosporamide A] which isn phase 3 cl...")
- 12:38, 4 July 2019 Matth talk contribs created page Actinoplanes (Created page with "In the family <em>[https://en.wikipedia.org/wiki/Micromonosporaceae Micromonosporaceae]</em> and best known for having motile spores.")
- 11:55, 4 July 2019 Matth talk contribs created page Saccharopolyspora (Created page with "In the family Pseudonocardiaceae along with [[<em>Amycolatopsis]]</em> and <em>Pseudonocardia</em>")
- 11:49, 4 July 2019 Matth talk contribs created page Amycolatopsis (Created page with "Circular genomes? Best known species is <em>A. orientalis</em> which makes the glycopeptiode vancomycin, used as a last resort drug against MRSA")
- 17:12, 3 July 2019 Alicia talk contribs created page Cloning and Expression of Gene Clusters (Created page with "TAR cloning Heterologous expression of gene clusters")
- 16:47, 3 July 2019 Alicia talk contribs created page High GC PCR (Created page with "Actinobacteria typically have a high GC content in their DNA. High GC DNA templates can be difficult to amplify by PCR because of stronger hydrogen bonding, and possible forma...")
- 15:55, 3 July 2019 ActinoCommittee talk contribs changed group membership for Alicia from (none) to administrator, interface administrator and bureaucrat
- 15:54, 3 July 2019 User account Alicia talk contribs was created by ActinoCommittee talk contribs
- 11:06, 3 July 2019 ActinoCommittee talk contribs changed group membership for DaveMark from (none) to administrator, interface administrator and bureaucrat
- 11:03, 3 July 2019 User account DaveMark talk contribs was created by ActinoCommittee talk contribs
- 09:21, 3 July 2019 User account Lucasbalis talk contribs was created by Tommclean talk contribs and password was sent by email (Setup)
- 09:12, 3 July 2019 Matth talk contribs uploaded File:ForBGC.png
- 09:12, 3 July 2019 Matth talk contribs created page File:ForBGC.png
- 09:03, 3 July 2019 ActinoCommittee talk contribs changed group membership for Tommclean from (none) to administrator, interface administrator and bureaucrat
- 09:01, 3 July 2019 User account Tommclean talk contribs was created by ActinoCommittee talk contribs (Its my first account)
- 13:27, 2 July 2019 MFeeney talk contribs created page Gause's No. 1 (Created page with "=Gause's No. 1 medium= ==Preparation-- Per litre: *20g soluble starch *1g KNO<sub>3</sub> *0.5g NaCl *0.5g K<sub>2</sub>HPO<sub>4</sub> *0.5g MgSO<sub>4</sub> *0.01g FeSO...")
- 12:01, 2 July 2019 ActinoCommittee talk contribs changed group membership for MFeeney from (none) to administrator
- 11:59, 2 July 2019 User account MFeeney talk contribs was created by ActinoCommittee talk contribs
- 11:04, 2 July 2019 Matth talk contribs created page Plasmids (Created page with "150px|Plasmids|link=Plasmids pSET152 pMS82")
- 10:44, 2 July 2019 Matth talk contribs uploaded File:Actinobase-icons-trans Protocols.png
- 10:44, 2 July 2019 Matth talk contribs created page File:Actinobase-icons-trans Protocols.png
- 10:44, 2 July 2019 Matth talk contribs uploaded File:Actinobase-icons-trans Organisms.png
- 10:44, 2 July 2019 Matth talk contribs created page File:Actinobase-icons-trans Organisms.png
- 10:43, 2 July 2019 Matth talk contribs uploaded File:Actinobase-icons-trans Plasmids.png
- 10:43, 2 July 2019 Matth talk contribs created page File:Actinobase-icons-trans Plasmids.png
- 10:43, 2 July 2019 Matth talk contribs uploaded File:Actinobase-icons-trans Media-recipes.png
- 10:43, 2 July 2019 Matth talk contribs created page File:Actinobase-icons-trans Media-recipes.png
- 10:43, 2 July 2019 Matth talk contribs uploaded File:Actinobase-icons-trans Background.png
- 10:43, 2 July 2019 Matth talk contribs created page File:Actinobase-icons-trans Background.png
- 10:40, 2 July 2019 Matth talk contribs uploaded File:StrepBible.png
- 10:40, 2 July 2019 Matth talk contribs created page File:StrepBible.png
- 16:56, 26 June 2019 User account Laia talk contribs was created by ActinoCommittee talk contribs
- 16:10, 26 June 2019 User account N Som talk contribs was created by Sam Prudence talk contribs
- 11:50, 26 June 2019 ActinoCommittee talk contribs created page MM (Created page with "=MM Medium= ==Preparation== Per litre: *0.5g L-asparagine *0.5g K2HPO4 *0.2g MgSO4*7H2O *0.01g FeSO4*7H2O *10g glucose (added after autoclaving) *10g agar *=1L dH2O '''Instr...")
- 14:58, 25 June 2019 ActinoCommittee talk contribs created page DNA (Created page with "=Difco Nutrient Agar (DNA)= Difco Nutrient Agar is a rich medium commercially available from BD and routinely used for cultivating many non-fastidious organisms. It contains...")
- 15:44, 24 June 2019 User account J Newitt talk contribs was created by Sam Prudence talk contribs
- 20:00, 20 June 2019 Sam Prudence talk contribs created page YEME (Created page with " =YEME Medium= ==For 1 Litre:== *3g Yeast Extract *3g Malt Extract *5g Peptone *10g Glucose *Up to 1L dH2O '''Instructions''' #Add 3g Yeast Extract, 3g Malt Extract, 5g Pept...")
- 19:54, 20 June 2019 Sam Prudence talk contribs created page LB (Created page with "=LB= Known as Lennox Broth or Lysogeny Broth ==For 1 Litre:== *5g Yeast Extract *10g NaCl *10g Tryptone *Up to 1L dH2O '''Instructions''' #Add 5g Yeast Extract, 10g NaCl and...")
- 19:39, 20 June 2019 Sam Prudence talk contribs created page PCR (Created page with "Streptomyces Colony PCR")
- 19:39, 20 June 2019 Sam Prudence talk contribs moved page General ''Streptomyces'' growth and maintenance to General growth and maintenance without leaving a redirect
- 19:38, 20 June 2019 Sam Prudence talk contribs moved page General Streptomyces growth and maintenance to General ''Streptomyces'' growth and maintenance without leaving a redirect
- 19:37, 20 June 2019 Sam Prudence talk contribs created page General Streptomyces growth and maintenance (Created page with "Spore Preparations & Titres Options for Growth in Liquid Medium")
- 19:24, 20 June 2019 Sam Prudence talk contribs created page DNB (Created page with "=Difco Nutrient Broth (DNB)= ==For 1 Litre:== *4g Difco Nutrient Broth Powder *10g Agar *1L dH2O water '''Instructions''' #For solid medium, add 0.8g of DNB powder into each...")
- 15:55, 20 June 2019 Matth talk contribs created page Streptomyces formicae (Created page with "Holmes NA, Devine R, Qin Z, Seipke RF, Wilkinson B, Hutchings MI (2018). Complete genome sequence of Streptomyces formicae KY5, the formicamycin producer. J Biotechnol. 265:11...")
- 15:34, 20 June 2019 Matth talk contribs created page 16S rRNA metaprofiling (Created page with "[https://greengenes.secondgenome.com Green Genes]")
- 15:33, 20 June 2019 Matth talk contribs created page Sequence databases (Created page with "[https://www.ncbi.nlm.nih.gov NCBI]")
- 15:31, 20 June 2019 Matth talk contribs created page DNA sequence analysis (Created page with "[http://meme-suite.org MEME: for identification of DNA binding sites in ChIP-seq data ]")
- 11:32, 20 June 2019 Sam Prudence talk contribs created page Admin contact page (Created page with "__NOTOC__ <h2><strong>How to contribute?</strong></h2> Actinobase is resource being built by the community and for the community. If you are an Actinomycete expert and would...")
- 11:21, 20 June 2019 Matth talk contribs created page Genomes (Created page with "[http://strepdb.streptomyces.org.uk/cgi-bin/dc3.pl?accession=AL645882&start=4291472&end=4302043&iorm=map&width=900 StrepDB]: the <em>Streptomyces</em> genome database curated...")
- 09:22, 20 June 2019 Matth talk contribs created page Bioinformatics (Created page with "Genome mining tools Protein & peptide analysis")
- 09:19, 20 June 2019 Matth talk contribs created page Suppliers (Created page with "Below is a list of useful suppliers and services: BioS&T https://www.biost.com")
- 08:25, 20 June 2019 Test talk contribs changed group membership for ActinoCommittee from interface administrator and administrator to interface administrator, administrator and bureaucrat
- 08:15, 20 June 2019 Test talk contribs changed group membership for Sam Prudence from interface administrator and administrator to interface administrator, administrator and bureaucrat
- 11:46, 19 June 2019 Matth talk contribs created page Links (Created page with "Genome mining tools")
- 09:21, 19 June 2019 Matth talk contribs created page Protocols (Created page with "RNA preps DNA preps RNA-seq ChIP-seq")
- 08:55, 19 June 2019 Matth talk contribs created page Pseudonocardia (Created page with "<em>Pseudonocardia</em> are filamentous actinomycetes with ~6Mbp circular genomes, best known as being vertically transmitted mutualists of attine ants. References:")
- 08:46, 19 June 2019 Matth talk contribs created page Organisms (Created page with "Amycolatopsis species Pseudonocardia species Saccharopolyspora species Streptomyces avermitilis Streptomyces coelicolor Streptomyces griseus Stretomyces venezuelae")
- 14:40, 13 June 2019 Andyxmas talk contribs created page MediaWiki:Sidebar (Created page with " * navigation ** mainpage|mainpage-description ** recentchanges-url|recentchanges ** randompage-url|randompage ** helppage|help-mediawiki * menu ** Background ** Media recipes...")
- 14:39, 13 June 2019 Andyxmas talk contribs created page Background (Created page with "...")
- 21:23, 8 June 2019 ActinoCommittee talk contribs created page Protein & peptide analysis (Created page with "Useful resources for analysing protein and peptide features, structures and families: <strong>STRING</strong>: https://string-db.org/ <strong>pFam</strong>: https://pfam.xfa...")
- 19:30, 8 June 2019 ActinoCommittee talk contribs created page Genome mining tools (Created page with "Actinobacteria are talented producers of secondary metabolites, many of which have useful biological activities. Thanks to the development of many targeted genome mining tools...")
- 19:13, 8 June 2019 ActinoCommittee talk contribs created page Useful tools (Created page with "Actinobacteria are talented producers of secondary metabolites, many of which have useful biological activities. Thanks to the development of targeted genome mining tools, we...")
- 18:42, 8 June 2019 ActinoCommittee talk contribs created page Streptomyces coelicolor (Created page with "<em>[[Streptomyces coelicolor</em> A3(2)]] <em>[[Streptomyces coelicolor</em> M1146]] <em>[[Streptomyces coelicolor</em> M1152]]")
- 18:03, 8 June 2019 ActinoCommittee talk contribs created page Streptomyces albus (Created page with "<em>Streptomyces albus</em> J1074 *<em>Streptomyces albus</em> J1074 is commonly used as a heterologous host for production of secondary metabolites *Its genome is the smalle...")
- 17:58, 8 June 2019 ActinoCommittee talk contribs created page Streptomyces albus J1074 (Created page with "<em>Streptomyces albus J1074</em> *<em>Streptomyces albus J1074</em> is commonly used as a heterologous host for expression of secondary metabolites. *Its genome is the small...")
- 17:50, 8 June 2019 ActinoCommittee talk contribs created page Streptomyces (Created page with "Streptomyces albus J1074")
- 17:40, 8 June 2019 ActinoCommittee talk contribs created page TSB (Created page with "=Tryptic Soy Broth= ==For 1 Litre:== *17g Tryptone *3g Soy *5g NaCl *2.5g dipotassium phosphate (K2HPO4) *2.5g glucose *1L with distilled water '''Instructions''' #Dissolve...")
- 10:02, 3 June 2019 Test talk contribs changed group membership for ActinoCommittee from (none) to administrator and interface administrator
- 10:02, 3 June 2019 Test talk contribs changed group membership for Matth from administrator to administrator and interface administrator
- 10:01, 3 June 2019 Test talk contribs changed group membership for Sam Prudence from (none) to administrator and interface administrator
- 10:01, 3 June 2019 Test talk contribs changed group membership for Matth from (none) to administrator
- 11:49, 30 May 2019 User account ActinoCommittee talk contribs was created by Test talk contribs
- 11:06, 30 May 2019 User account Sam Prudence talk contribs was created by Test talk contribs
- 12:59, 28 May 2019 User account Matth talk contribs was created by Test talk contribs
- 12:47, 28 May 2019 User account Hutch talk contribs was created by Test talk contribs and password was sent by email (To edit)
- 10:27, 24 May 2019 Test talk contribs created page MediaWiki:Common.css (Created page with "CSS placed here will be applied to all skins: * {font family: arial}")
- 14:28, 23 May 2019 Test talk contribs created page Media recipes (Created page with "Soya Flour Manniol Agar")
- 14:27, 23 May 2019 Test talk contribs created page Article (Created page with "Media recipes")
- 13:06, 23 May 2019 Andyxmas talk contribs changed group membership for Made from (none) to administrator, interface administrator and bureaucrat
- 13:04, 23 May 2019 User account Made talk contribs was created by Andyxmas talk contribs
- 16:25, 6 May 2019 Test talk contribs created page Streptomyces venezuelae (Created page with "==Organism Page== === ''Streptomyces venezuelae'' === Information not added")
- 16:22, 6 May 2019 Test talk contribs created page Trace elements (Created page with "=Trace Elements= Recipe not added")
- 16:13, 6 May 2019 Test talk contribs created page MYM (Created page with "=MYM Medium= ==For 1 Litre:== *4g Maltose *4g Yeast Extract Oxoid *10g Malt Extract *500ml Tap Water *500ml dH2O '''Instructions''' #Dissolve all ingredients in...")
- 15:05, 1 May 2019 Test talk contribs created page SFM (Created page with "=Soy Flour Mannitol Media= ==For 1 Litre:== *20g Mannitol *20g Soy Flour *20g Agar *Make up to 1L with tap water")
- 10:20, 1 May 2019 Test talk contribs created page ActinoBase:About (Created page with "ActinoBase is intended to be a community resource for scientists working on <em>Streptomyces</em> bacteria and other filamentous actinomycetes. Together these bacteria account...")
- 09:21, 1 May 2019 Test talk contribs uploaded a new version of File:Actinowiki.png
- 09:13, 1 May 2019 Test talk contribs uploaded File:Actinowiki.png
- 09:13, 1 May 2019 Test talk contribs created page File:Actinowiki.png
- 07:55, 1 May 2019 Andyxmas talk contribs changed group membership for Test from (none) to administrator, interface administrator and bureaucrat
- 07:50, 1 May 2019 User account Test talk contribs was created by Andyxmas talk contribs
- 14:49, 30 April 2019 Andyxmas talk contribs changed group membership for Matt from (none) to administrator, interface administrator and bureaucrat
- 14:48, 30 April 2019 User account Matt talk contribs was created by Andyxmas talk contribs and password was sent by email
- 08:57, 30 April 2019 Andyxmas talk contribs created page My cool stuff (Created page with "Here's my page about cool stuff. !")
- 08:46, 30 April 2019 Andyxmas talk contribs created page User:Andyxmas (Created page with "I'm Andy, and this is my page…")
- 08:37, 30 April 2019 MediaWiki default talk created page Main Page