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- 16:01, 20 August 2019 MFeeney talk contribs created page Mutagenesis (Created page with "A number of different methods have been developed for random and directed mutagenesis in actinobacteria (most notably for <em>Streptomyces coelicolor</em>). Many involve an in...")
- 16:25, 15 August 2019 S Worsley talk contribs created page RNA extraction from Actinobacteria (Created page with " =UNFINISHED PAGE: material is still being added= =Summary= This protocol for RNA extraction can be used to isolate large yields of high quality RNA from actinobacterial strai...")
- 15:12, 15 August 2019 User account S Worsley talk contribs was created by Sam Prudence talk contribs
- 10:34, 15 August 2019 Sam Prudence talk contribs created page File:Microsoc logo.png
- 10:34, 15 August 2019 Sam Prudence talk contribs uploaded File:Microsoc logo.png
- 14:44, 14 August 2019 J Newitt talk contribs created page Isolating Streptomyces from plant roots (Created page with "{{DISPLAYTITLE: Isolating ''Streptomyces'' from plant roots}}")
- 14:39, 14 August 2019 J Newitt talk contribs created page Isolating Actinomycetes from the environment (Created page with "Isolating ''Streptomyces'' from plant roots")
- 13:56, 13 August 2019 Alicia talk contribs uploaded a new version of File:PKC1132 Map.png
- 13:55, 13 August 2019 Alicia talk contribs created page File:PKC1132 Map.png
- 13:55, 13 August 2019 Alicia talk contribs uploaded File:PKC1132 Map.png
- 10:11, 13 August 2019 Andyxmas talk contribs created page Map (Created page with "<html> <iframe src="https://www.google.co.uk/maps/d/u/0/embed?mid=14bRNmsDo2vIXP_I8CVOgW4DJGB7ewkuf" width="640" height="480"></iframe> </html>")
- 09:50, 13 August 2019 Sam Prudence talk contribs moved page Salting Out DNA Extraction Method to Salting Out Genomic DNA Extraction Method
- 09:50, 13 August 2019 Sam Prudence talk contribs deleted page Salting Out Genomic DNA Extraction Method (Deleted to make way for move from "Salting Out DNA Extraction Method")
- 09:49, 13 August 2019 Sam Prudence talk contribs moved page Salting out method to Salting Out Genomic DNA Extraction Method
- 09:48, 13 August 2019 Sam Prudence talk contribs moved page Salting out method to Salting Out DNA Extraction Method
- 19:47, 12 August 2019 J Newitt talk contribs created page Salting out method (Created page with "=Isolation of high molecular weight (HMW) genomic DNA by salting out= The salting out method is used to isolate high yields of high molecular weight DNA, usually for applicat...")
- 08:22, 12 August 2019 Sam Prudence talk contribs moved page PCRISPomyces2 to PCRISPomyces-2
- 09:32, 9 August 2019 Alicia talk contribs created page File:PIJ10257 Map2.png
- 09:32, 9 August 2019 Alicia talk contribs uploaded File:PIJ10257 Map2.png
- 19:08, 8 August 2019 DaveMark talk contribs created page Contribution Guidelines (Created page with "==Intro==")
- 17:17, 8 August 2019 Alicia talk contribs created page File:PIJ10257 Map.png
- 17:17, 8 August 2019 Alicia talk contribs uploaded File:PIJ10257 Map.png
- 16:52, 8 August 2019 Sam Prudence talk contribs created page File:PCRISPomyces-2 map.png
- 16:52, 8 August 2019 Sam Prudence talk contribs uploaded File:PCRISPomyces-2 map.png
- 16:45, 8 August 2019 Sam Prudence talk contribs created page File:PCAP03 map.png
- 16:45, 8 August 2019 Sam Prudence talk contribs uploaded File:PCAP03 map.png
- 16:34, 8 August 2019 Sam Prudence talk contribs moved page PKT25 to PKT25/pKNT25
- 16:32, 8 August 2019 Sam Prudence talk contribs moved page PUT18 to PUT18/pUT18C
- 15:25, 8 August 2019 Sam Prudence talk contribs created page File:PRT802 Map.png
- 15:25, 8 August 2019 Sam Prudence talk contribs uploaded File:PRT802 Map.png
- 19:33, 3 August 2019 Sam Prudence talk contribs protected Test page [Create=Allow only administrators] (indefinite)
- 09:34, 3 August 2019 HeeeenaJ talk contribs created page ChIP Seq (Created page with "Buffers:- IP Buffer 50 mM HEPES pH to 7.5 with KOH 150 mM NaCl 1 mM EDTA 1 % Triton X-100 0.1 % Sodium deoxycholate 0.1 % SDS 5X Elution Buffer TE pH 7.6 5% SDS IP Buffer +...")
- 20:14, 2 August 2019 HeeeenaJ talk contribs created page PIJ12742 (Background)
- 12:45, 2 August 2019 Tommclean talk contribs created page Recruitment (Created page with "=Recruitment= Please feel free to post information and links to job opportunities etc below: *Please also remember to remove the links once filled.")
- 12:03, 2 August 2019 Matth talk contribs created page Groups (Created page with "Alphabetical list of research groups working on Streptomyces and closely related bacteria:")
- 10:35, 2 August 2019 User account R Devine talk contribs was created by Sam Prudence talk contribs
- 08:53, 2 August 2019 Sam Prudence talk contribs moved page Cloning and Expression of Gene Clusters to Cloning, Transformation and Expression of Gene Clusters
- 18:05, 1 August 2019 Sam Prudence talk contribs created page Tri-parental mating (Created page with "=<strong>Tri-parental Mating</strong>= ===Why Tri-Parental Mating?=== Tri-parental mating is useful for conjugating plasmids containing a kanamycin resistane gene as a selecta...")
- 15:57, 1 August 2019 Tommclean talk contribs created page SMMS (Created page with "=SMMS (Supplemented liquid minimal media, solid)= SMM is a solid supplemented minimal medium suitable for growth of many ''Streptomyces'' sp. ==Preparation== Per litre: *81.9...")
- 15:56, 1 August 2019 Jacob Hamilton talk contribs created page SPY (Created page with "=Salt Peptone Yeast Agar (SPY)= Salt Peptone Yeast agar is a metal-rich media used to stimulate the activation of cryptic biosynthetic gene clusters as part of antibiotic dis...")
- 15:55, 1 August 2019 Lucasbalis talk contribs created page Protein Methods (Created page with "Codon Optimising Sequences for Heterologous Expression in Streptomyces")
- 15:38, 1 August 2019 Jacob Hamilton talk contribs moved page Potato Dextrose Agar PDA to PDA (Rename)
- 15:36, 1 August 2019 Jacob Hamilton talk contribs moved page PGA to Potato Dextrose Agar PDA (Renaming from glucose to dextrose to maintain consistency)
- 15:33, 1 August 2019 Jacob Hamilton talk contribs created page PGA (Created page with "=Potato Dextrose Agar (PDA)= Potato Dextrose Agar is primarly used for the growth of the opportunistic fungal pathogen ''Scedosporium prolificans''. Thus, it is mostly used...")
- 15:30, 1 August 2019 Tommclean talk contribs created page SMM (Created page with "=SMM (Supplemented liquid minimal media)= Strauch et al., 1991<sup>1</sup> ==Preparation== Per litre: *81.9 mL PEG 6000 (BDH 6.1% w/v in distilled water) *2.5 mL Mg<sub>2</su...")
- 15:24, 1 August 2019 MFeeney talk contribs created page Overview of Streptomyces vectors (Created page with "150px|Plasmids|link=Plasmids =Commonly Used Streptomyces Vectors= This page describes the characteristics of some of the most co...")
- 15:21, 1 August 2019 Alicia talk contribs created page Streptomyces coelicolor A3(2) (Created page with "==Organism Page== === ''Streptomyces coelicolor A3(2)'' === ===Background=== ===Growth & Maintenance=== =====Sporulation===== *Sporulation can be achieved after 3-5 days gr...")
- 11:00, 1 August 2019 MFeeney talk contribs created page HT (Created page with "=Hickey-Tresner agar (HT)= This medium was developed by Hickey and Tresner<sup>[1]</sup> for the growth of some ''Streptomyces'' sp. that did not sporulate well on Bennett's...")
- 13:39, 31 July 2019 MFeeney talk contribs created page Antibiotic stocks and working concentrations (Created page with "{| class="wikitable" ! style="font-weight:bold; background-color:#c0c0c0;" | Antibiotic ! style="font-weight:bold; background-color:#c0c0c0;" | Stock [mg/ml] ! style="font-wei...")
- 13:29, 31 July 2019 User account Paulhoskisson talk contribs was created by MFeeney talk contribs and password was sent by email