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From ActinoBase
Combined display of all available logs of ActinoBase. You can narrow down the view by selecting a log type, the username (case-sensitive), or the affected page (also case-sensitive).
- 15:16, 17 October 2019 Alicia talk contribs created page Site-specific phage recombinase (Created page with "<strong>Integration sites</strong> You can check the sequence of your organism to see if it contains the appropriate integration site for your chosen plasmid: *ΦBT1, attB...")
- 15:44, 10 October 2019 J Newitt talk contribs created page Links master page (Created page with "=Useful Links= ==Bioinformatics== ===16S rRNA metaprofiling=== [https://greengenes.secondgenome.com Green Genes] 16S rRNA gene database [https://www.arb-silva.de/ SILVA]...")
- 16:25, 30 September 2019 DaveMark talk contribs created page Aminoglycosides (Created page with "=Intro= The aminoglycoside antibiotics are a class of amino containing oligosaccharides, and are notable for their broad-spectrum bacteriocidal activity - including their abil...")
- 14:35, 27 September 2019 LisAlgora talk contribs created page Southern Blotting (Created page with "There are many different protocols for Southern blotting. This approach uses vacuum transfer pf DNA to a nylon membrane, DIG-labelling of the probe and detection by chromogeni...")
- 13:53, 27 September 2019 EmilyAddington talk contribs created page Actinomycetes Morphology and Lifecycle (Created blank page)
- 08:57, 27 September 2019 User account JoshKlein talk contribs was created by MFeeney talk contribs and password was sent by email
- 08:56, 27 September 2019 User account LouCharkoudian talk contribs was created by MFeeney talk contribs and password was sent by email
- 16:01, 26 September 2019 J Newitt talk contribs created page General Growth and Maintenance of Streptomyces sp. (Created page with "=General growth and maintenance of ''Streptomyces'' species= ''Streptomyces'' are multi-nuclear filamentous organisms that undergo several different stages of growth. This se...")
- 15:25, 26 September 2019 Tommclean talk contribs created page Luria (Created page with "=Luria Broth= Known as Luria Broth (or sometimes, mistakenly, as LB or Lennox medium), this rich medium is used routinely for growth of <em>Escherichia coli</em>. There are se...")
- 15:25, 26 September 2019 Sam Prudence talk contribs created page File:SCOR2.jpg
- 15:25, 26 September 2019 Sam Prudence talk contribs uploaded File:SCOR2.jpg
- 15:23, 26 September 2019 Tommclean talk contribs created page Lennox (Created page with "=Lennox Broth= Known as Lennox Broth (or sometimes, mistakenly, as Luria-Bertani medium), this rich medium is used routinely for growth of <em>Escherichia coli</em>. There are...")
- 15:12, 26 September 2019 Tommclean talk contribs created page R5 (Created page with "=R5= ==Preparation== Make up the following solution: *103 g Sucrose *0.25 g K<sub>2</sub>SO<sub>4</sub> *10.12 g MgCl<sub>2</sub>.6H<sub>2</sub>O *10 g Glucose *0.1 g Difco...")
- 15:57, 12 September 2019 Sam Prudence talk contribs created page Isolating extracellular protein from Streptomyces (Created page with " =Isolation of extracellular protein from ''Streptomyces''= This protocol can be used to isolate membrance associated and secreted extracellular proteins from ''Streptomyces''...")
- 09:15, 11 September 2019 Sam Prudence talk contribs created page Test (Created page with "| rowspan="8" valign="top" width="120" | texty wexty ! colspan="2" align="left" | <font face="Arial Black" size="5" style="text-transform: uppercase;"> Heading A </font> | row...")
- 13:20, 4 September 2019 LisAlgora talk contribs created page Trace element solution (Created page with "'''Trace element solution (per liter):''' *40 mg ZnCl<sub>2</sub> *200 mg FeCl<sub>3</sub> . 6H<sub>2</sub>O *10 mg CuCl<sub>2</sub> . 2H<sub>2</sub>O *10 mg MnCl<sub>2</sub>...")
- 13:17, 4 September 2019 LisAlgora talk contribs created page L buffer (Created page with "'''L (lysis) buffer''' (Thompson ''et al.'' 1982) Mix the following sterile solutions: *100 ml Sucrose (10.3%) *10 ml TES buffer (5.73%, adjusted to pH7.2) *1 ml K<sub>2</su...")
- 12:51, 4 September 2019 LisAlgora talk contribs created page P buffer (Created page with "'''P (protoplast) buffer''' (Okanishi ''et al.'', 1974; Hopwood and Wright, 1978) Make up the following basal solution: *103 g Sucrose *0.25 g K<sub>2</sub>SO<sub>4</sub> *2....")
- 09:15, 4 September 2019 User account D Guillen talk contribs was created by Sam Prudence talk contribs
- 14:37, 2 September 2019 User account LeightonP talk contribs was created by MFeeney talk contribs and password was sent by email
- 04:37, 31 August 2019 H Otani talk contribs created page Rhodococcus jostii (Created page with "==Organism Page== === ''Rhodococcus jostii'' === ===Background=== ===Growth & Maintenance=== LB or M9 =====Sporulation===== Nonsporulating ===Strains=== <em>Rh...")
- 00:21, 31 August 2019 H Otani talk contribs created page Rhodococcus (Created page with "Rhodococci are nonsporulating actinomycetes in the family Nocardiaceae along with <em>Nocardia</em>. They are known for their ability to catabolise a wide range of organic...")
- 20:48, 30 August 2019 H Otani talk contribs created page Saccharopolyspora spinosa (Created page with "==Organism Page== === ''Saccharopolyspora spinosa'' === ===Background=== <em>Saccharopolyspora spinosa</em> is known for producing an insecticide, spinosad. Spinosad is a m...")
- 20:37, 30 August 2019 H Otani talk contribs created page YMPD (Created page with "=YMPD (Yeast Meat Peptone Dextrose)= For growth of ''Streptomyces griseus''. ==Preparation== Per litre: *2 g yeast extract *2.2 g meat extract *4 g Bacto peptone *5 g Na...")
- 15:40, 30 August 2019 EmilyAddington talk contribs uploaded a new version of File:Lifecycle.jpeg
- 15:36, 30 August 2019 EmilyAddington talk contribs created page File:Lifecycle.jpeg
- 15:36, 30 August 2019 EmilyAddington talk contribs uploaded File:Lifecycle.jpeg
- 15:28, 30 August 2019 LisAlgora talk contribs created page Protoplasts Formation, Regeneration and Transformation (Created page with "Protoplasts are used for fusion, transformation and transfection. =Protoplasts Preparation= Although this protocol was written for ''Streptomyces lividans'' and ''Str...")
- 15:03, 30 August 2019 ASBirke talk contribs created page Streptomyces clavuligerus (Created page with "=''Streptomyces clavuligerus''= ''Streptomyces clavuligerus'' was originally isolated from a South American soil sample for its ability to produce cephalosporins. Tod...")
- 15:01, 30 August 2019 DaveMark talk contribs created page File:Oligo 3.png
- 15:01, 30 August 2019 DaveMark talk contribs uploaded File:Oligo 3.png
- 14:40, 30 August 2019 MFeeney talk contribs created page Random mutagenesis using a mutator strain (Created page with "=<strong>Random mutagenesis using a mutator strain</strong>= This protocol has been confirmed to work for the following organisms. *<em>Streptomyces coelicolor</em> ==P...")
- 14:32, 30 August 2019 REMcHugh talk contribs created page Conjugation using ET12567/pUZ8002 (Created page with "#Transform ET12467/pUZ8002 with oriT-containing vector, and select for the incoming plasmid only #Inoculate a colony in to 10 ml LB containing Cm, Km and antibiotic used to se...")
- 13:57, 30 August 2019 EmilyAddington talk contribs created page Actinobacteria (Created page with "=Actinobacteria= ==General== ==Taxonomy== ==Ecology== ==Morphology and Lifecycle== ==Actinobacteria genetics== ==Antimicrobial production== ==References== ==Further Reading==")
- 13:53, 30 August 2019 LisAlgora talk contribs created page File:PIJ6902.gif
- 13:53, 30 August 2019 LisAlgora talk contribs uploaded File:PIJ6902.gif
- 13:48, 30 August 2019 LisAlgora talk contribs created page PIJ6902 (Created page with "{{DISPLAYTITLE: pIJ6092}} ==Use== ==Features== *Apramycin resistance cassette *φBT1 integration site & integrase ==History== ==Map== ==Sequence links== ==References=...")
- 13:47, 30 August 2019 User account EmilyAddington talk contribs was created by DaveMark talk contribs
- 13:20, 30 August 2019 User account Aros050 talk contribs was created by MFeeney talk contribs
- 10:52, 30 August 2019 User account D Udwary talk contribs was created by Sam Prudence talk contribs
- 10:48, 30 August 2019 User account H Otani talk contribs was created by Sam Prudence talk contribs
- 15:35, 29 August 2019 Tommclean talk contribs created page Exploration (Created page with "==General overview== <sup>[1]</sup> <strong>Useful host strains:</strong> *<em>Streptomyces venezuelae </em> <sup>[1]</sup> This species is currently the most well character...")
- 15:13, 29 August 2019 Tommclean talk contribs created page Characterisation of Actinomycetes (Created page with "Exploration Production of microbial volatile organic compounds")
- 14:50, 29 August 2019 User account Xtang talk contribs was created by Sam Prudence talk contribs
- 06:27, 29 August 2019 Yvonne Mast talk contribs created page Genome-based taxonomic analysis (Created page with "[https://tygs.dsmz.de/ TYGS] Type (Strain) Genome Server")
- 15:26, 27 August 2019 MFeeney talk contribs created page Teaching and Learning Resources: Antibiotics and AMR (Created page with "=Teaching and Learning Resources: Antibiotics and AMR= This section is our spotlight on teaching resources aimed for teaching about antibiotics and antimicrobial resistance at...")
- 11:14, 26 August 2019 Sam Prudence talk contribs protected Contribution Guidelines [Edit=Allow only administrators] (indefinite) [Move=Allow only administrators] (indefinite) (hist)
- 16:01, 20 August 2019 MFeeney talk contribs created page Mutagenesis (Created page with "A number of different methods have been developed for random and directed mutagenesis in actinobacteria (most notably for <em>Streptomyces coelicolor</em>). Many involve an in...")
- 16:25, 15 August 2019 S Worsley talk contribs created page RNA extraction from Actinobacteria (Created page with " =UNFINISHED PAGE: material is still being added= =Summary= This protocol for RNA extraction can be used to isolate large yields of high quality RNA from actinobacterial strai...")
- 15:12, 15 August 2019 User account S Worsley talk contribs was created by Sam Prudence talk contribs