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Combined display of all available logs of ActinoBase. You can narrow down the view by selecting a log type, the username (case-sensitive), or the affected page (also case-sensitive).
- 10:09, 5 May 2020 MFeeney talk contribs created page High titre preparation of phage (Created page with "=<strong>High titre preparation of ''Streptomyces'' phage</strong>= This protocol has been confirmed to work for the following organisms: *<em>Streptomyces coelicolor</e...")
- 09:47, 5 May 2020 MFeeney talk contribs created page File:PhiC31 serial dilution.jpg (Plaque assay: serial dilution of phiC31 on pglW- S. coelicolor)
- 09:47, 5 May 2020 MFeeney talk contribs uploaded File:PhiC31 serial dilution.jpg (Plaque assay: serial dilution of phiC31 on pglW- S. coelicolor)
- 09:02, 5 May 2020 MFeeney talk contribs created page Streptomyces Bacteriophage plaque assay (Created page with "=<strong>Streptomyces bacteriophage plaque assay</strong>= This protocol has been confirmed to work for the following organisms: *<em>Streptomyces coelicolor</em> with...")
- 08:34, 5 May 2020 MFeeney talk contribs created page File:PhiC31 plaque assay.jpg (PhiC31 plaque assay on a pglW- strain of S. coelicolor)
- 08:34, 5 May 2020 MFeeney talk contribs uploaded File:PhiC31 plaque assay.jpg (PhiC31 plaque assay on a pglW- strain of S. coelicolor)
- 08:31, 5 May 2020 MFeeney talk contribs deleted page File:PhiC31 serial dilution.jpg
- 08:31, 5 May 2020 MFeeney talk contribs created page File:PhiC31 serial dilution.jpg (PhiC31 plaque assay)
- 08:31, 5 May 2020 MFeeney talk contribs uploaded File:PhiC31 serial dilution.jpg (PhiC31 plaque assay)
- 22:37, 23 April 2020 LisAlgora talk contribs created page Lambda DNA/HindIII Ladder (Created page with "=Lambda HindIII Marker Preparation= *Lambda (λ) DNA (500 µg/ml): 100µl *10x Reaction Buffer: 45 µl *HindIII or PstI(10U/µl): 5µl *ddWater: 295µl Preparation: #Incubate...")
- 22:35, 23 April 2020 LisAlgora talk contribs created page Lambda DNA/HindIII ladder (Created page with "=Lambda HindIII Marker Preparation= *Lambda (λ) DNA (500 µg/ml): 100µl *10x Reaction Buffer: 45 µl *HindIII or PstI(10U/µl): 5µl *ddWater: 295µl Preparation: #Incubate...")
- 22:23, 23 April 2020 LisAlgora talk contribs created page Solutions for Southern Blotting (Created page with "=Stock Solutions to Prepare in Advance= {| class="wikitable" ! style="font-family:Arial, Helvetica, sans-serif !important;; background-color:rgb(222, 234, 246); color:rgb(0,...")
- 21:55, 23 April 2020 LisAlgora talk contribs created page Consumables for Southern Blotting (Created page with "Roche provides a <b>DIG DNA Labelling and Detection Kit</b> that contains: blocking reagent, Anti-DIG-AP conjugate, Hexanucleotide Mix, DIG DNA Labelling Mix, Klenow Enzyme an...")
- 09:03, 23 April 2020 MFeeney talk contribs moved page Bacteriophage to Streptomyces Bacteriophages without leaving a redirect
- 08:45, 23 April 2020 MFeeney talk contribs created page Bacteriophage (Created page with "=Streptomyces bacteriophages= Prof Paul A Hoskisson, University of Strathclyde. ==Introduction== Bacteriophages (or phages) are amongst the most abundant biological entities...")
- 12:51, 17 April 2020 Sam Prudence talk contribs changed group membership for MHutchings4 from (none) to administrator, interface administrator and bureaucrat
- 12:49, 17 April 2020 User account MHutchings4 talk contribs was created by Sam Prudence talk contribs
- 15:34, 16 April 2020 DaveMark talk contribs created page The ActinoBase e-Seminar (Created page with "=The ActinoBase e-Seminar Series= The ActinoBase e-Seminar Series is an opportunity for both early-career and established researchers in the Actinobacteria community to share...")
- 00:07, 25 March 2020 H Otani talk contribs created page RNA Sequencing (Created page with "#Extract and purify RNA samples according to RNA extraction from Actinobacteria. Add [https://www.thermofisher.com/order/catalog/product/4456740#/4456740 ERCC RNA Spike-In...")
- 23:43, 24 March 2020 H Otani talk contribs created page Natural Products (Created page with "Cloning, Transformation and Expression of Gene Clusters Metabolite extractions Production of microbial volatile organic compounds")
- 21:55, 24 March 2020 H Otani talk contribs created page Protoplast Transformation (Created page with "#Follow the instruction in Protoplasts Formation, Regeneration and Transformation #Add 3.85 ml P buffer to 1 g PEG sterilised. #Add 200 µl PEG-P buffer mixture and a DNA...")
- 17:27, 10 March 2020 Sam Prudence talk contribs created page File:Logo4.png
- 17:27, 10 March 2020 Sam Prudence talk contribs uploaded File:Logo4.png
- 17:17, 10 March 2020 Sam Prudence talk contribs created page File:ActinobaseLogo1.gif
- 17:17, 10 March 2020 Sam Prudence talk contribs uploaded File:ActinobaseLogo1.gif
- 15:09, 28 February 2020 MFeeney talk contribs created page Indicator compounds (Created page with "=Indicator Compounds= ==5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-Gal)== '''Uses''' *Detection of β-galactosidase activity on solid media *Bacterial two-hybrid a...")
- 14:33, 28 February 2020 DaveMark talk contribs created page File:PIJ12738 Map.png (Map of PIJ2738)
- 14:33, 28 February 2020 DaveMark talk contribs uploaded File:PIJ12738 Map.png (Map of PIJ2738)
- 14:06, 28 February 2020 User account Admb talk contribs was created by DaveMark talk contribs
- 15:31, 20 February 2020 User account GenomeBrowser talk contribs was created by Sam Prudence talk contribs
- 15:26, 20 February 2020 Sam Prudence talk contribs moved page Salinospora to Salinispora
- 15:22, 20 February 2020 Sam Prudence talk contribs created page Genome browser (Created page with "<h2><strong>ActinoBase Genome Browser</strong></h2> Click the link below for the ActinoBase MORF Genome Browser, with antiSMASH integration. This tool has been kindly provide...")
- 19:50, 16 February 2020 JoshKlein talk contribs created page File:BioArtElvis.PNG (BioArt photo from https://doi.org/10.1371/journal.pbio.1000510.)
- 19:50, 16 February 2020 JoshKlein talk contribs uploaded File:BioArtElvis.PNG (BioArt photo from https://doi.org/10.1371/journal.pbio.1000510.)
- 19:49, 16 February 2020 JoshKlein talk contribs created page File:BioArt.png (A picture of various BioArt plates.)
- 19:49, 16 February 2020 JoshKlein talk contribs uploaded File:BioArt.png (A picture of various BioArt plates.)
- 15:27, 31 January 2020 ElmiraM talk contribs created page Streptacidiphilus (Created page with "=<i>Streptacidiphilus</i>= Streptacidiphilus (etymology: twisted, acid-loving) are one of the three genera belonging to the <i>Streptomycetaceae<i> family, along with <i>Strep...")
- 15:23, 31 January 2020 MFeeney talk contribs created page Lambda-red mediated recombination using ssDNA (Created page with "=Lambda-red mediated recombination using ssDNA= Lambda-red mediated recombination using ssDNA can be used to introduce precise mutations into the genome of <em>Streptomyces</e...")
- 14:47, 31 January 2020 Jordanmurray talk contribs created page Cultivating Actinobacteria for Microscopic Analysis (Created page with "In order to analyse actinobacteria using light microscopy, methods must take into account the multicellular nature of the test organism. Unlike unicellular organisms, actinoba...")
- 16:31, 29 January 2020 R Devine talk contribs created page ChIP Sequencing (Created page with "==General overview== Chromatin Immunoprecipitation and sequencing can be used to characterise protein-DNA interactions. It works by cross-linking proteins to DNA, shearing t...")
- 16:56, 14 November 2019 Sam Prudence talk contribs deleted page Trace elements (content was: "=Trace Elements= Recipe not added", and the only contributor was "Test" (talk))
- 17:29, 7 November 2019 Sam Prudence talk contribs deleted page CRISPR (content before blanking was: "Hutchings Lab protocol for generating CRISPR/Cas Knockouts using pCRISPomyces-2. Written by Rebecca Devine, Matt Hutchings’ Laboratory, University of East Anglia, UK <strong>Overview (see following pages for more deta...")
- 14:19, 25 October 2019 DaveMark talk contribs created page Isolating Actinobacteria from soil environments (Created page with "Isolating Actinobacteria from soil environments =Equipment & Materials= *Sterile containers to transport the soil *A heat block *Plates of Isolation media *A vortexer *¼ Str...")
- 14:08, 25 October 2019 LeightonP talk contribs created page Using pandoc (add page describing use of pandoc to convert Word documents to MediaWiki format)
- 14:01, 25 October 2019 LeightonP talk contribs created page File:Example word file.png (Screenshot of example Word file, before pandoc conversion)
- 14:01, 25 October 2019 LeightonP talk contribs uploaded File:Example word file.png (Screenshot of example Word file, before pandoc conversion)
- 13:17, 25 October 2019 User account CharlieBegley talk contribs was created by DaveMark talk contribs
- 13:16, 25 October 2019 User account MegJeppesen talk contribs was created by DaveMark talk contribs (New Member)
- 13:15, 25 October 2019 User account MollyKeithBaker talk contribs was created by DaveMark talk contribs (New Member)
- 13:14, 25 October 2019 User account ElmiraM talk contribs was created by DaveMark talk contribs
- 15:16, 17 October 2019 Alicia talk contribs created page Site-specific phage recombinase (Created page with "<strong>Integration sites</strong> You can check the sequence of your organism to see if it contains the appropriate integration site for your chosen plasmid: *ΦBT1, attB...")
- 15:44, 10 October 2019 J Newitt talk contribs created page Links master page (Created page with "=Useful Links= ==Bioinformatics== ===16S rRNA metaprofiling=== [https://greengenes.secondgenome.com Green Genes] 16S rRNA gene database [https://www.arb-silva.de/ SILVA]...")
- 16:25, 30 September 2019 DaveMark talk contribs created page Aminoglycosides (Created page with "=Intro= The aminoglycoside antibiotics are a class of amino containing oligosaccharides, and are notable for their broad-spectrum bacteriocidal activity - including their abil...")
- 14:35, 27 September 2019 LisAlgora talk contribs created page Southern Blotting (Created page with "There are many different protocols for Southern blotting. This approach uses vacuum transfer pf DNA to a nylon membrane, DIG-labelling of the probe and detection by chromogeni...")
- 13:53, 27 September 2019 EmilyAddington talk contribs created page Actinomycetes Morphology and Lifecycle (Created blank page)
- 08:57, 27 September 2019 User account JoshKlein talk contribs was created by MFeeney talk contribs and password was sent by email
- 08:56, 27 September 2019 User account LouCharkoudian talk contribs was created by MFeeney talk contribs and password was sent by email
- 16:01, 26 September 2019 J Newitt talk contribs created page General Growth and Maintenance of Streptomyces sp. (Created page with "=General growth and maintenance of ''Streptomyces'' species= ''Streptomyces'' are multi-nuclear filamentous organisms that undergo several different stages of growth. This se...")
- 15:25, 26 September 2019 Tommclean talk contribs created page Luria (Created page with "=Luria Broth= Known as Luria Broth (or sometimes, mistakenly, as LB or Lennox medium), this rich medium is used routinely for growth of <em>Escherichia coli</em>. There are se...")
- 15:25, 26 September 2019 Sam Prudence talk contribs created page File:SCOR2.jpg
- 15:25, 26 September 2019 Sam Prudence talk contribs uploaded File:SCOR2.jpg
- 15:23, 26 September 2019 Tommclean talk contribs created page Lennox (Created page with "=Lennox Broth= Known as Lennox Broth (or sometimes, mistakenly, as Luria-Bertani medium), this rich medium is used routinely for growth of <em>Escherichia coli</em>. There are...")
- 15:12, 26 September 2019 Tommclean talk contribs created page R5 (Created page with "=R5= ==Preparation== Make up the following solution: *103 g Sucrose *0.25 g K<sub>2</sub>SO<sub>4</sub> *10.12 g MgCl<sub>2</sub>.6H<sub>2</sub>O *10 g Glucose *0.1 g Difco...")
- 15:57, 12 September 2019 Sam Prudence talk contribs created page Isolating extracellular protein from Streptomyces (Created page with " =Isolation of extracellular protein from ''Streptomyces''= This protocol can be used to isolate membrance associated and secreted extracellular proteins from ''Streptomyces''...")
- 09:15, 11 September 2019 Sam Prudence talk contribs created page Test (Created page with "| rowspan="8" valign="top" width="120" | texty wexty ! colspan="2" align="left" | <font face="Arial Black" size="5" style="text-transform: uppercase;"> Heading A </font> | row...")
- 13:20, 4 September 2019 LisAlgora talk contribs created page Trace element solution (Created page with "'''Trace element solution (per liter):''' *40 mg ZnCl<sub>2</sub> *200 mg FeCl<sub>3</sub> . 6H<sub>2</sub>O *10 mg CuCl<sub>2</sub> . 2H<sub>2</sub>O *10 mg MnCl<sub>2</sub>...")
- 13:17, 4 September 2019 LisAlgora talk contribs created page L buffer (Created page with "'''L (lysis) buffer''' (Thompson ''et al.'' 1982) Mix the following sterile solutions: *100 ml Sucrose (10.3%) *10 ml TES buffer (5.73%, adjusted to pH7.2) *1 ml K<sub>2</su...")
- 12:51, 4 September 2019 LisAlgora talk contribs created page P buffer (Created page with "'''P (protoplast) buffer''' (Okanishi ''et al.'', 1974; Hopwood and Wright, 1978) Make up the following basal solution: *103 g Sucrose *0.25 g K<sub>2</sub>SO<sub>4</sub> *2....")
- 09:15, 4 September 2019 User account D Guillen talk contribs was created by Sam Prudence talk contribs
- 14:37, 2 September 2019 User account LeightonP talk contribs was created by MFeeney talk contribs and password was sent by email
- 04:37, 31 August 2019 H Otani talk contribs created page Rhodococcus jostii (Created page with "==Organism Page== === ''Rhodococcus jostii'' === ===Background=== ===Growth & Maintenance=== LB or M9 =====Sporulation===== Nonsporulating ===Strains=== <em>Rh...")
- 00:21, 31 August 2019 H Otani talk contribs created page Rhodococcus (Created page with "Rhodococci are nonsporulating actinomycetes in the family Nocardiaceae along with <em>Nocardia</em>. They are known for their ability to catabolise a wide range of organic...")
- 20:48, 30 August 2019 H Otani talk contribs created page Saccharopolyspora spinosa (Created page with "==Organism Page== === ''Saccharopolyspora spinosa'' === ===Background=== <em>Saccharopolyspora spinosa</em> is known for producing an insecticide, spinosad. Spinosad is a m...")
- 20:37, 30 August 2019 H Otani talk contribs created page YMPD (Created page with "=YMPD (Yeast Meat Peptone Dextrose)= For growth of ''Streptomyces griseus''. ==Preparation== Per litre: *2 g yeast extract *2.2 g meat extract *4 g Bacto peptone *5 g Na...")
- 15:40, 30 August 2019 EmilyAddington talk contribs uploaded a new version of File:Lifecycle.jpeg
- 15:36, 30 August 2019 EmilyAddington talk contribs created page File:Lifecycle.jpeg
- 15:36, 30 August 2019 EmilyAddington talk contribs uploaded File:Lifecycle.jpeg
- 15:28, 30 August 2019 LisAlgora talk contribs created page Protoplasts Formation, Regeneration and Transformation (Created page with "Protoplasts are used for fusion, transformation and transfection. =Protoplasts Preparation= Although this protocol was written for ''Streptomyces lividans'' and ''Str...")
- 15:03, 30 August 2019 ASBirke talk contribs created page Streptomyces clavuligerus (Created page with "=''Streptomyces clavuligerus''= ''Streptomyces clavuligerus'' was originally isolated from a South American soil sample for its ability to produce cephalosporins. Tod...")
- 15:01, 30 August 2019 DaveMark talk contribs created page File:Oligo 3.png
- 15:01, 30 August 2019 DaveMark talk contribs uploaded File:Oligo 3.png
- 14:40, 30 August 2019 MFeeney talk contribs created page Random mutagenesis using a mutator strain (Created page with "=<strong>Random mutagenesis using a mutator strain</strong>= This protocol has been confirmed to work for the following organisms. *<em>Streptomyces coelicolor</em> ==P...")
- 14:32, 30 August 2019 REMcHugh talk contribs created page Conjugation using ET12567/pUZ8002 (Created page with "#Transform ET12467/pUZ8002 with oriT-containing vector, and select for the incoming plasmid only #Inoculate a colony in to 10 ml LB containing Cm, Km and antibiotic used to se...")
- 13:57, 30 August 2019 EmilyAddington talk contribs created page Actinobacteria (Created page with "=Actinobacteria= ==General== ==Taxonomy== ==Ecology== ==Morphology and Lifecycle== ==Actinobacteria genetics== ==Antimicrobial production== ==References== ==Further Reading==")
- 13:53, 30 August 2019 LisAlgora talk contribs created page File:PIJ6902.gif
- 13:53, 30 August 2019 LisAlgora talk contribs uploaded File:PIJ6902.gif
- 13:48, 30 August 2019 LisAlgora talk contribs created page PIJ6902 (Created page with "{{DISPLAYTITLE: pIJ6092}} ==Use== ==Features== *Apramycin resistance cassette *φBT1 integration site & integrase ==History== ==Map== ==Sequence links== ==References=...")
- 13:47, 30 August 2019 User account EmilyAddington talk contribs was created by DaveMark talk contribs
- 13:20, 30 August 2019 User account Aros050 talk contribs was created by MFeeney talk contribs
- 10:52, 30 August 2019 User account D Udwary talk contribs was created by Sam Prudence talk contribs
- 10:48, 30 August 2019 User account H Otani talk contribs was created by Sam Prudence talk contribs
- 15:35, 29 August 2019 Tommclean talk contribs created page Exploration (Created page with "==General overview== <sup>[1]</sup> <strong>Useful host strains:</strong> *<em>Streptomyces venezuelae </em> <sup>[1]</sup> This species is currently the most well character...")
- 15:13, 29 August 2019 Tommclean talk contribs created page Characterisation of Actinomycetes (Created page with "Exploration Production of microbial volatile organic compounds")
- 14:50, 29 August 2019 User account Xtang talk contribs was created by Sam Prudence talk contribs
- 06:27, 29 August 2019 Yvonne Mast talk contribs created page Genome-based taxonomic analysis (Created page with "[https://tygs.dsmz.de/ TYGS] Type (Strain) Genome Server")
- 15:26, 27 August 2019 MFeeney talk contribs created page Teaching and Learning Resources: Antibiotics and AMR (Created page with "=Teaching and Learning Resources: Antibiotics and AMR= This section is our spotlight on teaching resources aimed for teaching about antibiotics and antimicrobial resistance at...")
- 11:14, 26 August 2019 Sam Prudence talk contribs protected Contribution Guidelines [Edit=Allow only administrators] (indefinite) [Move=Allow only administrators] (indefinite) (hist)
- 16:01, 20 August 2019 MFeeney talk contribs created page Mutagenesis (Created page with "A number of different methods have been developed for random and directed mutagenesis in actinobacteria (most notably for <em>Streptomyces coelicolor</em>). Many involve an in...")
- 16:25, 15 August 2019 S Worsley talk contribs created page RNA extraction from Actinobacteria (Created page with " =UNFINISHED PAGE: material is still being added= =Summary= This protocol for RNA extraction can be used to isolate large yields of high quality RNA from actinobacterial strai...")
- 15:12, 15 August 2019 User account S Worsley talk contribs was created by Sam Prudence talk contribs