VProtocols

Here you will find links to video protocols created by our members. We hope you find these useful. Please send feedback by emailing Matt Hutchings

These are video protocols recorded by Dr Lorena Fernández-Martínez at EdgeHill University. Email Lorena

Streptomyces spore preparation

Conjugation from E. coli to Streptomyces species

These are video protocols recorded by Dr Jake Newitt of the John Innes Centre for the Microbiology Society Small World initiative which was part of the global citizen science project Antibiotics Unearthed. Email Jake

Introduction to the Small World Lab Diaries

01. Isolating bacteria from soil

02. Examining your bacterial colonies

03. Identifying Streptomyces colonies

04. Purifying Streptomyces colonies from mixed cultures - streaking out

05. Single colonies of Streptomyces.

06. DNA amplification explained

07. How to amplify DNA from your bacterial colonies

08. Checking your DNA using agarose gel electrophoresis

09. Sequencing the DNA to identify your bacterial strain

10. BIoassaying Streptomyces bacteria for antibacterial activity

Other useful links:

JOVE - the Journal of Visualised Experiments have published a number of Streptomyces protocols that can be found here.

AddGene have a YouTube channel with useful video protocols.