YEME: Difference between revisions
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*10g Glucose | *10g Glucose | ||
*340 g sucrose (34% final concentration) | *340 g sucrose (34% final concentration) | ||
*Up to 1L | *Up to 1L dH<sub>2</sub>O | ||
'''Instructions''' | '''Instructions''' | ||
#Add 3g Yeast Extract, 3g Malt Extract, 5g Peptone and 10g Glucose to a beaker | #Add 3g Yeast Extract, 3g Malt Extract, 5g Peptone and 10g Glucose to a beaker | ||
#Add 800ml | #Add 800ml dH<sub>2</sub>O and stir until all solids are fully suspended | ||
#Make up to 1L with | #Make up to 1L with dH<sub>2</sub>O | ||
#Autoclave | #Autoclave | ||
#After autoclaving, add 2mL | #After autoclaving, add 2mL MgCl<sub>2</sub>*6H<sub>2</sub>O (2.5M) - (5mM final concentration) | ||
#For preparing protoplasts, also add 25mL 20% glycine (0.5% final concentration) | #For preparing protoplasts, also add 25mL 20% glycine (0.5% final concentration) | ||
Latest revision as of 12:56, 2 July 2019
YEME Medium
Preparation
Per litre:
- 3g Yeast Extract
- 3g Malt Extract (Oxoid)
- 5g Peptone
- 10g Glucose
- 340 g sucrose (34% final concentration)
- Up to 1L dH2O
Instructions
- Add 3g Yeast Extract, 3g Malt Extract, 5g Peptone and 10g Glucose to a beaker
- Add 800ml dH2O and stir until all solids are fully suspended
- Make up to 1L with dH2O
- Autoclave
- After autoclaving, add 2mL MgCl2*6H2O (2.5M) - (5mM final concentration)
- For preparing protoplasts, also add 25mL 20% glycine (0.5% final concentration)
Uses
Notes
References
Kieser, T., Bibb, M.J., Buttner, M.J., Chater, K.F. and Hopwood, D.A. (2000) Practical Streptomyces Genetics: John Innes Foundation, Norwich Research Park, Colney, Norwich NR4 7UH, UK
