YEME

YEME Medium

Preparation

Per litre:

• 3g Yeast Extract
• 3g Malt Extract (Oxoid)
• 5g Peptone
• 10g Glucose
• 340 g sucrose (34% final concentration)
• Up to 1L dH₂O

Instructions

1. Add 3g Yeast Extract, 3g Malt Extract, 5g Peptone and 10g Glucose to a beaker
2. Add 800ml dH₂O and stir until all solids are fully suspended
3. Make up to 1L with dH₂O
4. Autoclave
5. After autoclaving, add 2mL MgCl₂*6H₂O (2.5M) - (5mM final concentration)
6. For preparing protoplasts, also add 25mL 20% glycine (0.5% final concentration)

References

Kieser, T., Bibb, M.J., Buttner, M.J., Chater, K.F. and Hopwood, D.A. (2000) Practical Streptomyces Genetics: John Innes Foundation, Norwich Research Park, Colney, Norwich NR4 7UH, UK