ActinoBase - User contributions [en-gb]

SPY

2019-08-01T15:56:45Z

Jacob Hamilton:

=Salt Peptone Yeast Agar (SPY)=

Salt Peptone Yeast agar is a metal-rich media used to stimulate the activation of cryptic biosynthetic gene clusters as part of antibiotic discovery.

==Preparation==
Per Litre:
*30g Soluable Starch
*15g pancreatic digest of casein
*15g peptic digest of animal tissue
*2g Yeast Extract
*10g MgSO4
*50mg CaCl2
*30mg ZnSO4
*25mg FeSO4
*16mg CuSO4
*12mg MnSO4
*20g agar
*NaOH (to adust pH)
*1L dH2O

'''Instructions'''
#On a heated stiring block, warm the water and add the pancreatice digest of casin, the peptic digest of animal tissue, the yeast extract and the trace elements.
#Add 2g of agar into each of 10 250mL conical flasks.
#Use NaOH to adjust the pH of the solution to pH 7.2.
#Dissolve the soluable starch in the solution. It can take a while for the starch to dissolve.
#Pour 100mL of solution into each flask.
#Autoclave

==Uses==

==Notes==
*To dissolve the soluable starch, it is often required to make the media on a hot plate. Do not allow the solution to boil.
*To add the trace elements, it may be easier to make concentrated stock solution which is then added to the media prior to pH adjustment.

==References==
[https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4421039/ Imai, Y., Sato, S., Tanaka, Y., Ochi, K. and Hosaka, T. (2015). Lincomycin at Subinhibitory Concentrations Potentiates Secondary Metabolite Production by Streptomyces spp. Applied and Environmental Microbiology, 81(11), pp.3869-3879.]

SPY

2019-08-01T15:56:18Z

Jacob Hamilton: Created page with "=Salt Peptone Yeast Agar (SPY)= Salt Peptone Yeast agar is a metal-rich media used to stimulate the activation of cryptic biosynthetic gene clusters as part of antibiotic dis..."

=Salt Peptone Yeast Agar (SPY)=

Salt Peptone Yeast agar is a metal-rich media used to stimulate the activation of cryptic biosynthetic gene clusters as part of antibiotic discovery.

==Preparation==
Per Litre:
*30g Soluable Starch
*15g pancreatic digest of casein
*15g peptic digest of animal tissue
*2g Yeast Extract
*10g MgSO4
*50mg CaCl2
*30mg ZnSO4
*25mg FeSO4
*16mg CuSO4
*12mg MnSO4
*20g agar
*NaOH (to adust pH)
*1L dH2O

'''Instructions'''
#On a heated stiring block, warm the water and add the pancreatice digest of casin, the peptic digest of animal tissue, the yeast extract and the trace elements.
#Add 2g of agar into each of 10 250mL conical flasks.
#Use NaOH to adjust the pH of the solution to pH 7.2.
#Dissolve the soluable starch in the solution. It can take a while for the starch to dissolve.
#Pour 100mL of solution into each flask.
#Autoclave

==Uses==

==Notes==
*To dissolve the soluable starch, it is often required to make the media on a hot plate. Do not allow the solution to boil.
*To add the trace elements, it may be easier to make concentrated stock solution which is then added to the media prior to pH adjustment.

==References==
DOI: [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4421039/ Imai, Y., Sato, S., Tanaka, Y., Ochi, K. and Hosaka, T. (2015). Lincomycin at Subinhibitory Concentrations Potentiates Secondary Metabolite Production by Streptomyces spp. Applied and Environmental Microbiology, 81(11), pp.3869-3879.]

PDA

2019-08-01T15:39:47Z

Jacob Hamilton: /* Preparation */

=Potato Dextrose Agar (PDA)=

Potato Dextrose Agar is primarly used for the growth of the opportunistic fungal pathogen ''Scedosporium prolificans''. Thus, it is mostly used in determining the bioactivity of actinomycete strains against ''Scedosporium''.

==Preparation==
Per Litre:
*4g Potato Extract
*20g Dextrose
*20g Agar
*1L dH2O

'''Instructions'''
#Dissolve the potato extract and dextrose in the dH2O
#Add 2g of agar into each of 10 250mL conical flasks
#Into each flask, pour 100mL of the potato extract/dextrose solution
#Autoclave

==Uses==
*Bioactivity of actinomycetes against ''S. prolificans''. When being used in this manner, spot the actinomycete in the centre of the petri dish and incubate until sporulation. Then, streak a single straight line of ''S. prolificans'' along one side of the petri dish. Leave the plate 'upside down' (i.e. with the incolulated side of th media facing upwards) in a dark location. After a few days, look to see if the actinomycete has caused the ''S. prolificans'' to grow in a cresent shape or if the bacterial colony has been encroached upon by the fungus.

*Bioactivity of actinomycetes against the Take All fungus (''Gaeumannomyces graminis var. tritici''), in a similar method to that described above.

==Notes==

==References==

Potato Dextrose Agar PDA

2019-08-01T15:38:53Z

Jacob Hamilton: Jacob Hamilton moved page Potato Dextrose Agar PDA to PDA: Rename

#REDIRECT [[PDA]]

PDA

2019-08-01T15:38:52Z

Jacob Hamilton: Jacob Hamilton moved page Potato Dextrose Agar PDA to PDA: Rename

=Potato Dextrose Agar (PDA)=

Potato Dextrose Agar is primarly used for the growth of the opportunistic fungal pathogen ''Scedosporium prolificans''. Thus, it is mostly used in determining the bioactivity of actinomycete strains against ''Scedosporium''.

==Preparation==
Per Litre:
*4g Potato Extract
*20g Dextrose
*20g Agar
*1L dH2O

'''Instructions'''
#Dissolve the potato extract and dextrose in the dH2O
#Into 10 250mL conical flasks, add 2g of agar to each flask
#Into each flask, pour 100mL of the potato extract/dextrose solution
#Autoclave

==Uses==
*Bioactivity of actinomycetes against ''S. prolificans''. When being used in this manner, spot the actinomycete in the centre of the petri dish and incubate until sporulation. Then, streak a single straight line of ''S. prolificans'' along one side of the petri dish. Leave the plate 'upside down' (i.e. with the incolulated side of th media facing upwards) in a dark location. After a few days, look to see if the actinomycete has caused the ''S. prolificans'' to grow in a cresent shape or if the bacterial colony has been encroached upon by the fungus.

*Bioactivity of actinomycetes against the Take All fungus (''Gaeumannomyces graminis var. tritici''), in a similar method to that described above.

==Notes==

==References==

Media recipes

2019-08-01T15:37:39Z

Jacob Hamilton: /* Media Recipes */

{{DISPLAYTITLE:Media Recipes}}

[[File:Actinobase-icons-trans_Media-recipes.png|150px|Media Recipes|link=Media_recipes]]

=Media Recipes=

[[A1]]

[[CM]] (Complete medium)

[[DNA]] (Difco Nutrient Agar) | [[DNB]] (Difco Nutrient Broth) | [[SNA]] (Soft Nutrient Agar)

[[Emerson medium]]

[[Gause's No. 1]]

[[GYM]] (Glucose + Yeast Extract + Malt Extract)

[[HT]] (Hickey-Tresner)

[[HV]]

[[IMA]] (Smash)

[[ISP2]]

[[ISP3]]

[[ISP4]]

[[ISP5]]

[[ISP6]]

[[ISP7]]

[[LB]] (Lysogeny Broth) | [[L]] (L agar)

[[MC agar]] (Milk Casein Agar)

[[MM]] (Minimal medium)

[[SFM | MS]] (Mannitol Soya agar - a.k.a. Soya Flour Mannitol)

[[MYM]] (Maltose + Yeast Extract + Malt Extract)

[[NMMP]] (minimal liquid medium)

[[OT]] (oligotrophic agar)

[[PDA]] (Potato Dextrose Agar)

[[R2]] | [[R2YE]] | [[R5]]

[[SC]] (Starch Casein agar)

[[SFM]] (Soya Flour Mannitol - a.k.a. Mannitol Soya agar)

[[SMM]] (supplemented liquid minimal medium) | [[SMMS]] (supplemented minimal medium, solid)

[[SPY]] (Salt Peptone Yeast)

[[TSB]] (Tryptone Soy Broth)

[[YEME]] (Yeast Extract + Malt Extract)

[[YP]] (Yeast Peptone) | [[YPD]] (Yeast Peptone Dextrose)

[[2X YT]]

==Media Supplements==

[[Antibiotic stocks and working concentrations]]

[[Indicator compounds]]

[[Media supplements for activating cryptic biosynthetic gene clusters]]

[[Media supplements for induction gene expression from inducible promoters]]

PGA

2019-08-01T15:36:35Z

Jacob Hamilton: Jacob Hamilton moved page PGA to Potato Dextrose Agar PDA: Renaming from glucose to dextrose to maintain consistency

#REDIRECT [[Potato Dextrose Agar PDA]]

PDA

2019-08-01T15:36:34Z

Jacob Hamilton: Jacob Hamilton moved page PGA to Potato Dextrose Agar PDA: Renaming from glucose to dextrose to maintain consistency

=Potato Dextrose Agar (PDA)=

Potato Dextrose Agar is primarly used for the growth of the opportunistic fungal pathogen ''Scedosporium prolificans''. Thus, it is mostly used in determining the bioactivity of actinomycete strains against ''Scedosporium''.

==Preparation==
Per Litre:
*4g Potato Extract
*20g Dextrose
*20g Agar
*1L dH2O

'''Instructions'''
#Dissolve the potato extract and dextrose in the dH2O
#Into 10 250mL conical flasks, add 2g of agar to each flask
#Into each flask, pour 100mL of the potato extract/dextrose solution
#Autoclave

==Uses==
*Bioactivity of actinomycetes against ''S. prolificans''. When being used in this manner, spot the actinomycete in the centre of the petri dish and incubate until sporulation. Then, streak a single straight line of ''S. prolificans'' along one side of the petri dish. Leave the plate 'upside down' (i.e. with the incolulated side of th media facing upwards) in a dark location. After a few days, look to see if the actinomycete has caused the ''S. prolificans'' to grow in a cresent shape or if the bacterial colony has been encroached upon by the fungus.

*Bioactivity of actinomycetes against the Take All fungus (''Gaeumannomyces graminis var. tritici''), in a similar method to that described above.

==Notes==

==References==

PDA

2019-08-01T15:34:11Z

Jacob Hamilton: /* Uses */

=Potato Dextrose Agar (PDA)=

Potato Dextrose Agar is primarly used for the growth of the opportunistic fungal pathogen ''Scedosporium prolificans''. Thus, it is mostly used in determining the bioactivity of actinomycete strains against ''Scedosporium''.

==Preparation==
Per Litre:
*4g Potato Extract
*20g Dextrose
*20g Agar
*1L dH2O

'''Instructions'''
#Dissolve the potato extract and dextrose in the dH2O
#Into 10 250mL conical flasks, add 2g of agar to each flask
#Into each flask, pour 100mL of the potato extract/dextrose solution
#Autoclave

==Uses==
*Bioactivity of actinomycetes against ''S. prolificans''. When being used in this manner, spot the actinomycete in the centre of the petri dish and incubate until sporulation. Then, streak a single straight line of ''S. prolificans'' along one side of the petri dish. Leave the plate 'upside down' (i.e. with the incolulated side of th media facing upwards) in a dark location. After a few days, look to see if the actinomycete has caused the ''S. prolificans'' to grow in a cresent shape or if the bacterial colony has been encroached upon by the fungus.

*Bioactivity of actinomycetes against the Take All fungus (''Gaeumannomyces graminis var. tritici''), in a similar method to that described above.

==Notes==

==References==

PDA

2019-08-01T15:33:28Z

Jacob Hamilton: Created page with "=Potato Dextrose Agar (PDA)= Potato Dextrose Agar is primarly used for the growth of the opportunistic fungal pathogen ''Scedosporium prolificans''. Thus, it is mostly used..."

=Potato Dextrose Agar (PDA)=

Potato Dextrose Agar is primarly used for the growth of the opportunistic fungal pathogen ''Scedosporium prolificans''. Thus, it is mostly used in determining the bioactivity of actinomycete strains against ''Scedosporium''.

==Preparation==
Per Litre:
*4g Potato Extract
*20g Dextrose
*20g Agar
*1L dH2O

'''Instructions'''
#Dissolve the potato extract and dextrose in the dH2O
#Into 10 250mL conical flasks, add 2g of agar to each flask
#Into each flask, pour 100mL of the potato extract/dextrose solution
#Autoclave

==Uses==
*Bioactivity of actinomycetes against ''S. prolificans''. When being used in this manner, spot the actinomycete in the centre of the petri dish and incubate for until sporulation. Then, streak a single straight line of ''S. prolificans'' along one side of the petri dish. Leave the plate 'upside down' (i.e. with the incolulated side of th media facing upwards) in a dark location. After a few days, look to see if the actinomycete has caused the ''S. prolificans'' to grow in a cresent shape or if the bacterial colony has been encroached upon by the fungus.

*Bioactivity of actinomycetes against the Take All fungus (''Gaeumannomyces graminis var. tritici''), in a similar method to that described above.

==Notes==

==References==