ActinoBase - User contributions [en-gb]

PSET152

2026-10-10T09:28:37Z

Matt Hutchings: /* References */

{{DISPLAYTITLE: pSET152}}

==Features==

• Backbone: Derived from a standard E. coli plasmid vector (pBR322/pUC lineage) containing an E. coli replication origin (ori) and an origin of transfer (oriT) derived from broad-host-range plasmid RK2 to allow conjugal transfer.

• Integrative Machinery: Incorporates the attachment site (attP) and the integrase gene (int) from the temperate actinophage φC31. Integrates into the φC31 att site on the Streptomyces genome.

• Selection Marker: Carries the aac(3)IV gene, which confers resistance to apramycin in both E. coli and Streptomyces.

==History==

The pSET152 plasmid was constructed and published by Bierman et al. in 1992 as an E. coli–Streptomyces shuttle and integrative vector

==Map==
[[File:pSET152.png]]

==Sequence links==

==References==

M. Bierman, R. Logan, K. O'Brien, E.T. Seno, R. Nagaraja Rao, B.E. Schoner (1992). Plasmid cloning vectors for the conjugal transfer of DNA from Escherichia coli to Streptomyces spp. Gene 116:43-49.

Abstract
We have constructed cloning vectors for the conjugal transfer of DNA from Escherichia coli to Streptomyces spp. All vectors contain the 760-bp oriT fragment from the IncP plasmid, RK2. Transfer functions need to be supplied in trans by the E. coli donor strain. We have incorporated into these vectors selectable antibiotic-resistance markers (AmR, ThR, SpR) that function in Streptomyces spp. and other features that should allow for: (i) integration via homologous recombination between cloned DNA and the Streptomyces spp. chromosome, (ii) autonomous replication, or (iii) site-specific integration at the bacteriophage φC31 attachment site. Shuttle cosmids for constructing genomic libraries and bacteriophage P1 cloning vector capable of accepting approx. 100-kb fragments are also described. A simple mating procedure has been developed for the conjugal transfer of these vectors from E. coli to Streptomyces spp. that involves plating of the donor strain and either germinated spores or mycelial fragments of the recipient strain. We have shown that several of these vectors can be introduced into Streptomyces fradiae, a strain that is notoriously difficult to transform by PEG-mediated protoplast transformation.

https://www.sciencedirect.com/science/article/abs/pii/0378111992906272

PSET152

2026-10-10T09:26:06Z

Matt Hutchings: /* Features */

PSET152

2026-10-10T09:24:57Z

Matt Hutchings:

{{DISPLAYTITLE: pSET152}}

==Features==

• Backbone: Derived from a standard E. coli plasmid vector (such as pBR322/pUC lineage) containing an E. coli replication origin (ori) and an origin of transfer (oriT) derived from a broad-host-range plasmid like RK2 to allow conjugal transfer.

• Integrative Machinery: Incorporates the attachment site (attP) and the integrase gene (int) from the temperate actinophage φC31.

• Selection Marker: Carries the aac(3)IV gene, which confers resistance to apramycin in both E. coli and Streptomyces.

==History==

The pSET152 plasmid was constructed and published by Bierman et al. in 1992 as an E. coli–Streptomyces shuttle and integrative vector

==Map==
[[File:pSET152.png]]

==Sequence links==

==References==

PSET152

2026-10-10T09:24:33Z

Matt Hutchings:

PSET152

2026-10-10T09:23:44Z

Matt Hutchings: /* Use */

{{DISPLAYTITLE: pSET152}}

Key Components & Origin

• Backbone: Derived from a standard E. coli plasmid vector (such as pBR322/pUC lineage) containing an E. coli replication origin (ori) and an origin of transfer (oriT) derived from a broad-host-range plasmid like RK2 to allow conjugal transfer.

• Integrative Machinery: Incorporates the attachment site (attP) and the integrase gene (int) from the temperate actinophage φC31.

• Selection Marker: Carries the aac(3)IV gene, which confers resistance to apramycin in both E. coli and Streptomyces.

==Features==

Apramycin resistance marker allows for selection of the plasmid after conjkugation from E. coli

==History==

==Map==
[[File:pSET152.png]]

==Sequence links==

==References==

PSET152

2026-10-10T09:22:37Z

Matt Hutchings: /* Features */

{{DISPLAYTITLE: pSET152}}

==Use==

Integrating genes in trans into the phi C31 phage attachment site. This can be used for complementation of mutants with wild-type alleles under native, constitutive or inducible promoters or alleles encoding Flag-tagged proteins for ChIP-seq and co-IP.

==Features==

Apramycin resistance marker allows for selection of the plasmid after conjkugation from E. coli

==History==

==Map==
[[File:pSET152.png]]

==Sequence links==

==References==

PSET152

2026-10-10T09:22:03Z

Matt Hutchings: /* Use */

{{DISPLAYTITLE: pSET152}}

==Use==

Integrating genes in trans into the phi C31 phage attachment site. This can be used for complementation of mutants with wild-type alleles under native, constitutive or inducible promoters or alleles encoding Flag-tagged proteins for ChIP-seq and co-IP.

==Features==

==History==

==Map==
[[File:pSET152.png]]

==Sequence links==

==References==

PSET152

2026-10-10T09:21:03Z

Matt Hutchings: /* Use */

{{DISPLAYTITLE: pSET152}}

==Use==

Integrating genes in trans into the phi C31 ohage attachment site

==Features==

==History==

==Map==
[[File:pSET152.png]]

==Sequence links==

==References==

Methods

2026-09-30T10:32:52Z

Matt Hutchings: Blanked the page

File:PSS150.png

2026-03-02T11:00:25Z

Matt Hutchings:

PSS150

2026-03-02T11:00:11Z

Matt Hutchings:

pSS150: pIJ10257 carrying PermE*-sfgfp, HygR

Codon-optimised sfgfp was inserted between the NdeI/XhoI of pIJ10257

Reference:
Casu B, Sallmen JW, Schlimpert S, Pilhofer M. Cytoplasmic contractile injection systems mediate cell death in Streptomyces. Nat Microbiol. 2023 Apr;8(4):711-726. doi: 10.1038/s41564-023-01341-x. Epub 2023 Mar 9. PMID: 36894633; PMCID: PMC10066040.

[[File:pSS150.png|options|caption]]

PSS150

2026-03-02T10:45:01Z

Matt Hutchings: Created page with "pSS150: pIJ10257 carrying PermE*-sfgfp, HygR Codon-optimised sfgfp was inserted between the NdeI/XhoI of pIJ10257 Reference: Casu B, Sallmen JW, Schlimpert S, Pilhofer M. Cytoplasmic contractile injection systems mediate cell death in Streptomyces. Nat Microbiol. 2023 Apr;8(4):711-726. doi: 10.1038/s41564-023-01341-x. Epub 2023 Mar 9. PMID: 36894633; PMCID: PMC10066040."

Plasmids

2026-03-02T10:44:34Z

Matt Hutchings: /* Plasmids used in Streptomyces */

[[File:Actinobase-icons-trans_Plasmids.png|150px|Plasmids|link=Plasmids]]

Various plasmids have been developed for use in Streptomyces (most will also contain an origin of replication that functions in Escherichia coli, for cloning, general maintenance, and [[intergeneric conjugation into Streptomyces]]). Many of the plasmids commonly used for Streptomyces work are '''integrative''': they insert into a specific site on the Streptomyces chromosome using a [[site-specific phage recombinase]].

The following is an alphabetical list of a number of useful vectors - see also [[Overview of Streptomyces vectors]]. Some of these vectors are available under MTA from [https://www.addgene.org/ Addgene]. Many others are also available under MTA by request at [mailto:jic.strepstrains@jic.ac.uk|JIC StrepStrains].

==Plasmids used in Streptomyces==

[[pIJ86]]

[[pCAP03]]

[[pCRISPomyces-2]]

[[pCRISPR-Cas9]]

[[pCRISPR-dCas9]]

[[pESAC13]]

[[pGM9]]

[[pGM190]]

[[pIJ101]]

[[pIJ6021]]

[[pIJ8660]]

[[pIJ10257]]

[[pIJ12738]]

[[pIJ12742]]

[[pIJ6902]]

[[pKC1132]]

[[pMF96]]

[[pMS82]]

[[pRT802]]

[[pSET152]]

[[pSS150]]

[[pSS170]]

[[pTOYAMAcos]]

[[pTYM18]]

[[pTYM19]]

[[pUZ8002]] - conjugation helper plasmid

[[Supercos1]]

==Plasmids used for PCR targeting (Redirect)==

[http://streptomyces.org.uk/redirect/index.html Redirect protocol and template information]

[[pIJ773]]

==Plasmids used for heterologous protein expression in E. coli==

The following plasmids are frequently used to express proteins heterologously in E. coli, e.g. for two-hybrid analysis or protein purification.

[[pET28a]]

Vectors for Bacterial two-hybrid experiments
[[pKT25/pKNT25]] | [[pUT18/pUT18C]]

MM

2026-03-02T10:10:36Z

Matt Hutchings: /* Preparation */

=MM (Minimal Medium)=
MM is a minimal medium suitable for growth of many Streptomyces sp (S. coelicolor and S. venezuelae both grow well on it). It is often made up with glucose or mannitol, but other carbon sources can be used. It can be further supplemented with additional growth factors as described below.

==Preparation==
Per litre:
*0.5g L-asparagine
*0.5g K2HPO4
*0.2g MgSO4*7H2O
*0.01g FeSO4*7H2O
*10g glucose (added after autoclaving)
*20g agar
*1L dH2O

'''Instructions'''
#Dissolve all of the ingredients except the agar and the glucose in the distilled water
#Adjust the pH to 7.0-7.2
#Dispense 200ml into flasks each containing 2g agar
#Autoclave
#After autoclaving, add 4 ml sterile 50% glucose solution (autoclaved separately or filter-sterilized) to each flask
#Add any other growth factors required for auxotrophic mutants

==Uses==
*Studies of auxotrophic mutants and carbon source utilization.

==Notes==
The glucose can be replaced by alternative carbon sources such as glycerol, arabinose, or mannitol (each at 5g/L). Mannitol is often preferable to glucose for optimal growth and development.

To study carbon source utilization, the L-asparagine should be replaced by ammonium sulfate (5 g/L(NH4)2SO4) - to avoid confusion due to utilization of asparagine as a carbon source.

Additional supplements can be added as follows (amount/L): amino acids except histidine, 50 mg; histidine, 70 mg; adenine and uracil, 10 mg; vitamins, 1 mg; dihydrostreptomycin sulfate, 50 mg; acriflavine, 15 mg. (Hopwood 1967)

==References==
Hopwood DA. Genetic analysis and genome structure in Streptomyces coelicolor. Bacteriol Rev. 1967;31(4):373–403.

Kieser, T., Bibb, M.J., Buttner, M.J., Chater, K.F. and Hopwood, D.A. (2000) Practical Streptomyces Genetics: John Innes Foundation, Norwich Research Park, Colney, Norwich NR4 7UH, UK

HV

2026-03-02T10:08:12Z

Matt Hutchings: /* Preparation */

=HV=

==Preparation==
Per Litre:
*1g humic acid
*1.7g KCl
*0.5g Na2HPO4
*0.5g MgSO4
*0.02g CaCO3
*0.01g FeSO4
*1mL VB stock solution (added after autoclaving)
*20g agar
*1L dH2O

'''Instructions'''
#Combine all components (except for the VB stock solution) and mix well.
#Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
#Add 1ml VB stock solution, mix well and pour into sterile Petri dishes.

==Uses==
*[[Isolation experiments]]

==Notes==
VB stock solution: 50mg VB1 (thiamine), VB2 (riboflavin), VB3 (niacin), VB6 (pyridoxine), B5 (D-calcium pantothenate), inositol and PABA (para amino acid), 25mg B7 (Biotin),and 100mL distilled water).

==References==
Hayakawa and Nonomura (1987). Humic acid-vitamin agar, a new medium for the selective isolation of soil actinomycetes. Journal of Fermentation Technology 65(5): 501-509.

GYM

2026-03-02T10:07:51Z

Matt Hutchings: /* Preparation */

=GYM=

==Preparation==
Per litre:
*4 g Glucose
*4 g Yeast Extract
*10 g Malt Extract
*2 g CaCO3
*20 g Agar
*1 L dH2O

'''Instructions'''

#Dissolve constituents in 1L dH2O
#Adjust pH to 7.2
#Add agar

==Uses==

==Notes==
*CaCO3 is only needed for solid media, don't add to liquid

==References==

Gause's No. 1

2026-03-02T10:07:33Z

Matt Hutchings: /* Preparation */

=Gause's No. 1 medium=

==Preparation==
Per litre:
*20g soluble starch
*1g KNO3
*0.5g NaCl
*0.5g K2HPO4
*0.5g MgSO4
*0.01g FeSO4
*20g agar

==Uses==

==Notes==

==References==

Emerson medium

2026-03-02T10:07:12Z

Matt Hutchings: /* Preparation */

=Emerson medium=

Emerson medium is a rich medium formulated by Emerson et al (1) to isolate mould and Actinobacteria. It is composed of beef extract, yeast extract, peptic digest of animal tissue, dextrose and sodium chloride.
This medium is also used for the maintenance of mould and actinobacteria pure cultures.

==Preparation==
Per litre of distilled water:
Beef extract 4.0 g
Yeast extract 1.0 g
Peptic digest of animal tissue 4.0 g
Dextrose 10.0 g
Sodium chloride 2.5 g
Agar 20 g
Final pH (at 25°C) 7.0

'''Instructions'''
Mix well all the components in 1000 mL of distilled water. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Mix well and pour into sterile Petri dishes.

==Uses==
*[[Actinobacteria maintenance]]
*[[Mould maintenance]]
*[[Fungi maintenance]]
*[[Isolation experiments]]
*[[Induce antibiotic production of antibiotic producing organisms]]

==Notes==
For growth in liquid culture, Emerson Agar can be made without the agar.

==References==
EMERSON, R. L. and WHIFFEN, A. J. (1946) ‘Studies on the production of antibiotics by actinomycetes and molds.’, Journal of bacteriology.

DNA

2026-03-02T10:06:47Z

Matt Hutchings: /* Preparation */

=Difco Nutrient Agar (DNA)=

Difco Nutrient Agar is a rich medium commercially available from BD and routinely used for cultivating many non-fastidious organisms. It contains beef extract and peptones; exact composition will vary between batches.

Many ''Streptomyces'' sp. grow well on DNA, but developmental and/or antibiotic production phenotypes may differ compared to growth on other media (e.g. [[SFM]]).

==Preparation==
Per Litre:
*4g Difco Nutrient Broth Powder
*20g Agar
*1L dH2O

'''Instructions'''
#For solid medium, add 0.8g of DNB powder into each 250ml conical flask
#Add 4g of Agar
#Add 200ml dH2O
#Autoclave

==Uses==
*''Streptomyces'' Colony PCR (see [[High GC PCR]] for instructions)
*[[Spore titres]]
*[[Re-isolation experiments]]
*[[Selection for resistance to salt-sensitive antibiotics]]
*[[Streptomyces Bacteriophage plaque assay]]

==Notes==
For growth in liquid culture, [[DNB]] can be made without the agar.

According to the recipe in Practical Streptomyces Genetics, it should be 8g of DNB per litre, however some groups choose to use 4g instead. This has little effect on the ability of ''Streptomyces'' to grow on the soild medium, [[DNA]].

==References==

CM

2026-03-02T10:06:28Z

Matt Hutchings: /* Preparation */

=CM (Complete Medium)=

==Preparation==
Per litre:
*5g K2HPO4
*0.5g NaCl
*0.5g MgSO4*7H2O
*2g Difco Bacto-peptone
*1.5g Difco Casaminoacids
*50mg each L-histidine, L-proline, L-tryptophan, and L-tyrosine*
*5mL Yeast nucleic acid hydrolysate
*1mL vitamin solution
*25g glucose
*20g agar
*1L dH2O

Yeast nucleic acid hydrolysate
#Boil 2g yeast nucleic acid in 15mL 1N HCl for 10 min.
#Boil 2g yeast nucleic acid in 15mL 1N NaOH for 10 min.
#Mix the two solutions and adjust to pH6
#Filter through filter paper while hot
#Make up the volume to 40mL with water
#Do not sterilize

Vitamin solution
*100mg riboglavine
*100mg nicotinamide
*10mg p-aminobenzoic acid
*50mg pyridoxine HCl
*50mg thiamine HCl
*20 mg biotin
*100mL water

'''Instructions'''
#Make up the yeast nucleic acid hydrolysate according to the directions above: do not sterilize. Make up the vitamin solution following the recipe above and sterilize by autoclaving (115˚C, 10 min).
#Dissolve all of the ingredients, except the agar and the vitamin solution, but including the glucose, in the distilled water
#Adjust the pH to 7.2 with 1N HCl
#Add the vitamin solution
#Dispense 200ml into flasks each containing 40g agar
#Autoclave

==Uses==
*A non-selective medium useful for genetic crosses (the progeny from such crosses can subsequently be analysed on appropriate selective media such as [[MM]])[1]

==Notes==
*The histidine, proline, tryptophan, and tyrosine should be added for the relevant auxotrophic mutants; if not working with auxotrophs, any/all of these amino acids can be omitted.

==References==
[1] Hopwood DA. Genetic analysis and genome structure in Streptomyces coelicolor. Bacteriol Rev. 1967;31(4):373–403.

[2] Kieser, T., Bibb, M.J., Buttner, M.J., Chater, K.F. and Hopwood, D.A. (2000) Practical Streptomyces Genetics: John Innes Foundation, Norwich Research Park, Colney, Norwich NR4 7UH, UK

Media recipes

2026-03-02T10:06:04Z

Matt Hutchings: /* Media Recipes */

{{DISPLAYTITLE:Media Recipes}}

[[File:Actinobase-icons-trans_Media-recipes.png|150px|Media Recipes|link=Media_recipes]]

=Media Recipes=

Please note that 15 or 20g/L (1.5-2%) agar is added to make solid medium

[[ABB13]]

[[A1]]

[[CM]] (Complete medium)

[[DNA]] (Difco Nutrient Agar) | [[DNB]] (Difco Nutrient Broth) | [[SNA]] (Soft Nutrient Agar)

[[Emerson medium]]

[[Gause's No. 1]]

[[GYM]] (Glucose + Yeast Extract + Malt Extract)

[[HT]] (Hickey-Tresner)

[[HV]]

[[IMA]] (Smash)

[[LB]] (Lysogeny Broth) | [[Lennox]] (Lennox agar) | [[Luria]] (Luria agar)

[[MC agar]] (Milk Casein Agar)

[[MM]] (Minimal medium)

[[SFM | MS]] (Mannitol Soya agar - a.k.a. Soya Flour Mannitol (SFM))

[[1/2 MS0 | 1/2 MS-S]]

[[1/2 MS+B5]]

[[MYM]] (Maltose + Yeast Extract + Malt Extract)

[[NMMP]] (minimal liquid medium)

[[OT]] (oligotrophic agar)

[[PDA]] (Potato Dextrose Agar)

[[R2]] | [[R2YE]] | [[R5]]

[[SC]] (Starch Casein agar)

[[SFM]] (Soya Flour Mannitol - a.k.a. Mannitol Soya agar)

[[SMM]] (supplemented liquid minimal medium) | [[SMMS]] (supplemented minimal medium, solid)

[[SNA]] (Soft nutrient agar)

[[SPY]] (Salt Peptone Yeast)

[[TSB]] (Tryptone Soy Broth)

[[YEME]] (Yeast Extract + Malt Extract)

[[YMPD]] (Yeast Meat Peptone Dextrose)

[[YMS]] (Yeast-Malt extract-Soluble starch medium)

[[YP]] (Yeast Peptone) | [[YPD]] (Yeast Peptone Dextrose)

[[2X YT]]

====International ''Streptomyces'' Project Media (ISP's)====

[[ISP2]]

[[ISP3]]

[[ISP4]]

[[ISP5]]

[[ISP6]]

[[ISP7]]

==Media Supplements==

[[Antibiotic stocks and working concentrations]]

[[Indicator compounds]]

[[Media supplements for activating cryptic biosynthetic gene clusters]]

[[Media supplements for induction gene expression from inducible promoters]]

[[Trace element solution]]

Media recipes

2026-03-02T10:05:40Z

Matt Hutchings: /* Media Recipes */

{{DISPLAYTITLE:Media Recipes}}

[[File:Actinobase-icons-trans_Media-recipes.png|150px|Media Recipes|link=Media_recipes]]

=Media Recipes=

Please note that 20g/L (2%) agar is added to make solid medium

[[ABB13]]

[[A1]]

[[CM]] (Complete medium)

[[DNA]] (Difco Nutrient Agar) | [[DNB]] (Difco Nutrient Broth) | [[SNA]] (Soft Nutrient Agar)

[[Emerson medium]]

[[Gause's No. 1]]

[[GYM]] (Glucose + Yeast Extract + Malt Extract)

[[HT]] (Hickey-Tresner)

[[HV]]

[[IMA]] (Smash)

[[LB]] (Lysogeny Broth) | [[Lennox]] (Lennox agar) | [[Luria]] (Luria agar)

[[MC agar]] (Milk Casein Agar)

[[MM]] (Minimal medium)

[[SFM | MS]] (Mannitol Soya agar - a.k.a. Soya Flour Mannitol (SFM))

[[1/2 MS0 | 1/2 MS-S]]

[[1/2 MS+B5]]

[[MYM]] (Maltose + Yeast Extract + Malt Extract)

[[NMMP]] (minimal liquid medium)

[[OT]] (oligotrophic agar)

[[PDA]] (Potato Dextrose Agar)

[[R2]] | [[R2YE]] | [[R5]]

[[SC]] (Starch Casein agar)

[[SFM]] (Soya Flour Mannitol - a.k.a. Mannitol Soya agar)

[[SMM]] (supplemented liquid minimal medium) | [[SMMS]] (supplemented minimal medium, solid)

[[SNA]] (Soft nutrient agar)

[[SPY]] (Salt Peptone Yeast)

[[TSB]] (Tryptone Soy Broth)

[[YEME]] (Yeast Extract + Malt Extract)

[[YMPD]] (Yeast Meat Peptone Dextrose)

[[YMS]] (Yeast-Malt extract-Soluble starch medium)

[[YP]] (Yeast Peptone) | [[YPD]] (Yeast Peptone Dextrose)

[[2X YT]]

====International ''Streptomyces'' Project Media (ISP's)====

[[ISP2]]

[[ISP3]]

[[ISP4]]

[[ISP5]]

[[ISP6]]

[[ISP7]]

==Media Supplements==

[[Antibiotic stocks and working concentrations]]

[[Indicator compounds]]

[[Media supplements for activating cryptic biosynthetic gene clusters]]

[[Media supplements for induction gene expression from inducible promoters]]

[[Trace element solution]]

Emerson medium

2026-03-02T10:04:55Z

Matt Hutchings: /* Preparation */

=Emerson medium=

Emerson medium is a rich medium formulated by Emerson et al (1) to isolate mould and Actinobacteria. It is composed of beef extract, yeast extract, peptic digest of animal tissue, dextrose and sodium chloride.
This medium is also used for the maintenance of mould and actinobacteria pure cultures.

==Preparation==
Per litre of distilled water:
Beef extract 4.0 g
Yeast extract 1.0 g
Peptic digest of animal tissue 4.0 g
Dextrose 10.0 g
Sodium chloride 2.5 g
Agar 200 g
Final pH (at 25°C) 7.0

'''Instructions'''
Mix well all the components in 1000 mL of distilled water. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Mix well and pour into sterile Petri dishes.

==Uses==
*[[Actinobacteria maintenance]]
*[[Mould maintenance]]
*[[Fungi maintenance]]
*[[Isolation experiments]]
*[[Induce antibiotic production of antibiotic producing organisms]]

==Notes==
For growth in liquid culture, Emerson Agar can be made without the agar.

==References==
EMERSON, R. L. and WHIFFEN, A. J. (1946) ‘Studies on the production of antibiotics by actinomycetes and molds.’, Journal of bacteriology.

DNA

2026-03-02T10:04:36Z

Matt Hutchings: /* Preparation */

=Difco Nutrient Agar (DNA)=

Difco Nutrient Agar is a rich medium commercially available from BD and routinely used for cultivating many non-fastidious organisms. It contains beef extract and peptones; exact composition will vary between batches.

Many ''Streptomyces'' sp. grow well on DNA, but developmental and/or antibiotic production phenotypes may differ compared to growth on other media (e.g. [[SFM]]).

==Preparation==
Per Litre:
*4g Difco Nutrient Broth Powder
*200g Agar
*1L dH2O

'''Instructions'''
#For solid medium, add 0.8g of DNB powder into each 250ml conical flask
#Add 40g of Agar
#Add 200ml dH2O
#Autoclave

==Uses==
*''Streptomyces'' Colony PCR (see [[High GC PCR]] for instructions)
*[[Spore titres]]
*[[Re-isolation experiments]]
*[[Selection for resistance to salt-sensitive antibiotics]]
*[[Streptomyces Bacteriophage plaque assay]]

==Notes==
For growth in liquid culture, [[DNB]] can be made without the agar.

According to the recipe in Practical Streptomyces Genetics, it should be 8g of DNB per litre, however some groups choose to use 4g instead. This has little effect on the ability of ''Streptomyces'' to grow on the soild medium, [[DNA]].

==References==

CM

2026-03-02T09:55:20Z

Matt Hutchings:

=CM (Complete Medium)=

==Preparation==
Per litre:
*5g K2HPO4
*0.5g NaCl
*0.5g MgSO4*7H2O
*2g Difco Bacto-peptone
*1.5g Difco Casaminoacids
*50mg each L-histidine, L-proline, L-tryptophan, and L-tyrosine*
*5mL Yeast nucleic acid hydrolysate
*1mL vitamin solution
*25g glucose
*200g agar
*1L dH2O

Yeast nucleic acid hydrolysate
#Boil 2g yeast nucleic acid in 15mL 1N HCl for 10 min.
#Boil 2g yeast nucleic acid in 15mL 1N NaOH for 10 min.
#Mix the two solutions and adjust to pH6
#Filter through filter paper while hot
#Make up the volume to 40mL with water
#Do not sterilize

Vitamin solution
*100mg riboglavine
*100mg nicotinamide
*10mg p-aminobenzoic acid
*50mg pyridoxine HCl
*50mg thiamine HCl
*20 mg biotin
*100mL water

'''Instructions'''
#Make up the yeast nucleic acid hydrolysate according to the directions above: do not sterilize. Make up the vitamin solution following the recipe above and sterilize by autoclaving (115˚C, 10 min).
#Dissolve all of the ingredients, except the agar and the vitamin solution, but including the glucose, in the distilled water
#Adjust the pH to 7.2 with 1N HCl
#Add the vitamin solution
#Dispense 200ml into flasks each containing 40g agar
#Autoclave

==Uses==
*A non-selective medium useful for genetic crosses (the progeny from such crosses can subsequently be analysed on appropriate selective media such as [[MM]])[1]

==Notes==
*The histidine, proline, tryptophan, and tyrosine should be added for the relevant auxotrophic mutants; if not working with auxotrophs, any/all of these amino acids can be omitted.

==References==
[1] Hopwood DA. Genetic analysis and genome structure in Streptomyces coelicolor. Bacteriol Rev. 1967;31(4):373–403.

[2] Kieser, T., Bibb, M.J., Buttner, M.J., Chater, K.F. and Hopwood, D.A. (2000) Practical Streptomyces Genetics: John Innes Foundation, Norwich Research Park, Colney, Norwich NR4 7UH, UK

Media recipes

2026-03-02T09:54:18Z

Matt Hutchings: /* Media Recipes */

{{DISPLAYTITLE:Media Recipes}}

[[File:Actinobase-icons-trans_Media-recipes.png|150px|Media Recipes|link=Media_recipes]]

=Media Recipes=

Please note that 200g/L (20%) agar is added to make solid medium

[[ABB13]]

[[A1]]

[[CM]] (Complete medium)

[[DNA]] (Difco Nutrient Agar) | [[DNB]] (Difco Nutrient Broth) | [[SNA]] (Soft Nutrient Agar)

[[Emerson medium]]

[[Gause's No. 1]]

[[GYM]] (Glucose + Yeast Extract + Malt Extract)

[[HT]] (Hickey-Tresner)

[[HV]]

[[IMA]] (Smash)

[[LB]] (Lysogeny Broth) | [[Lennox]] (Lennox agar) | [[Luria]] (Luria agar)

[[MC agar]] (Milk Casein Agar)

[[MM]] (Minimal medium)

[[SFM | MS]] (Mannitol Soya agar - a.k.a. Soya Flour Mannitol (SFM))

[[1/2 MS0 | 1/2 MS-S]]

[[1/2 MS+B5]]

[[MYM]] (Maltose + Yeast Extract + Malt Extract)

[[NMMP]] (minimal liquid medium)

[[OT]] (oligotrophic agar)

[[PDA]] (Potato Dextrose Agar)

[[R2]] | [[R2YE]] | [[R5]]

[[SC]] (Starch Casein agar)

[[SFM]] (Soya Flour Mannitol - a.k.a. Mannitol Soya agar)

[[SMM]] (supplemented liquid minimal medium) | [[SMMS]] (supplemented minimal medium, solid)

[[SNA]] (Soft nutrient agar)

[[SPY]] (Salt Peptone Yeast)

[[TSB]] (Tryptone Soy Broth)

[[YEME]] (Yeast Extract + Malt Extract)

[[YMPD]] (Yeast Meat Peptone Dextrose)

[[YMS]] (Yeast-Malt extract-Soluble starch medium)

[[YP]] (Yeast Peptone) | [[YPD]] (Yeast Peptone Dextrose)

[[2X YT]]

====International ''Streptomyces'' Project Media (ISP's)====

[[ISP2]]

[[ISP3]]

[[ISP4]]

[[ISP5]]

[[ISP6]]

[[ISP7]]

==Media Supplements==

[[Antibiotic stocks and working concentrations]]

[[Indicator compounds]]

[[Media supplements for activating cryptic biosynthetic gene clusters]]

[[Media supplements for induction gene expression from inducible promoters]]

[[Trace element solution]]

Media recipes

2026-02-24T11:14:34Z

Matt Hutchings: /* Media Recipes */

{{DISPLAYTITLE:Media Recipes}}

[[File:Actinobase-icons-trans_Media-recipes.png|150px|Media Recipes|link=Media_recipes]]

=Media Recipes=

Please note that 200g/L agar is typically added to make solid medium

[[ABB13]]

[[A1]]

[[CM]] (Complete medium)

[[DNA]] (Difco Nutrient Agar) | [[DNB]] (Difco Nutrient Broth) | [[SNA]] (Soft Nutrient Agar)

[[Emerson medium]]

[[Gause's No. 1]]

[[GYM]] (Glucose + Yeast Extract + Malt Extract)

[[HT]] (Hickey-Tresner)

[[HV]]

[[IMA]] (Smash)

[[LB]] (Lysogeny Broth) | [[Lennox]] (Lennox agar) | [[Luria]] (Luria agar)

[[MC agar]] (Milk Casein Agar)

[[MM]] (Minimal medium)

[[SFM | MS]] (Mannitol Soya agar - a.k.a. Soya Flour Mannitol (SFM))

[[1/2 MS0 | 1/2 MS-S]]

[[1/2 MS+B5]]

[[MYM]] (Maltose + Yeast Extract + Malt Extract)

[[NMMP]] (minimal liquid medium)

[[OT]] (oligotrophic agar)

[[PDA]] (Potato Dextrose Agar)

[[R2]] | [[R2YE]] | [[R5]]

[[SC]] (Starch Casein agar)

[[SFM]] (Soya Flour Mannitol - a.k.a. Mannitol Soya agar)

[[SMM]] (supplemented liquid minimal medium) | [[SMMS]] (supplemented minimal medium, solid)

[[SNA]] (Soft nutrient agar)

[[SPY]] (Salt Peptone Yeast)

[[TSB]] (Tryptone Soy Broth)

[[YEME]] (Yeast Extract + Malt Extract)

[[YMPD]] (Yeast Meat Peptone Dextrose)

[[YMS]] (Yeast-Malt extract-Soluble starch medium)

[[YP]] (Yeast Peptone) | [[YPD]] (Yeast Peptone Dextrose)

[[2X YT]]

====International ''Streptomyces'' Project Media (ISP's)====

[[ISP2]]

[[ISP3]]

[[ISP4]]

[[ISP5]]

[[ISP6]]

[[ISP7]]

==Media Supplements==

[[Antibiotic stocks and working concentrations]]

[[Indicator compounds]]

[[Media supplements for activating cryptic biosynthetic gene clusters]]

[[Media supplements for induction gene expression from inducible promoters]]

[[Trace element solution]]

ABB13

2026-01-27T12:44:32Z

Matt Hutchings:

ABB13 is a great medium for inducing antibiotic production and has also been used to improve transformation efficiency in actinomycetes.

ABB1 base medium contained the following components (g/L):
*10g Bacto Soytone,
*9g yeast extract,
*10g soluble starch,
*15g cerelose
*Add pH 7.0 filter sterilised MOPS buffer to a final concentration of 50 mM after autoclaving.

References

Fitzgerald et al (1998). Sonication-Dependent Electroporation of the Erythromycin-Producing Bacterium Saccharopolyspora erythraea. AEM 674:1580–1583. https://journals.asm.org/doi/10.1128/aem.64.4.1580-1583.1998

ABB13

2026-01-27T12:41:51Z

Matt Hutchings:

ABB13 is a great medium for inducing antibiotic production and has also been used to improve transformation efficiency in actinomycetes.

ABB1 base medium contained the following components (g/L):
*10g Bacto Soytone,
*9g yeast extract,
*10g soluble starch,
*15g cerelose
*Filter sterilised 3-N-morpholinopropanesulfonic acid (MOPS) buffer, pH 7.0, was added to o a final concentration of 50 mM after autoclaving.

References

Fitzgerald et al (1998). Sonication-Dependent Electroporation of the Erythromycin-Producing Bacterium Saccharopolyspora erythraea. AEM 674:1580–1583. https://journals.asm.org/doi/10.1128/aem.64.4.1580-1583.1998

ABB13

2026-01-27T12:41:17Z

Matt Hutchings:

ABB13 is a great medium for inducing antibiotic production and has also been used to improve transformation efficiency in actinomycetes.

ABB1 base medium contained the following components (g/L):
*10g Bacto Soytone,
*9g yeast extract,
*10g soluble starch,
*15g cerelose
*Filter sterilised 3-N-morpholinopropanesulfonic acid (MOPS) buffer, pH 7.0, was added to ABB1 base medium after autoclaving to a final concentration of 50 mM.

References

Fitzgerald et al (1998). Sonication-Dependent Electroporation of the Erythromycin-Producing Bacterium Saccharopolyspora erythraea. AEM 674:1580–1583. https://journals.asm.org/doi/10.1128/aem.64.4.1580-1583.1998

ABB13

2026-01-27T12:40:00Z

Matt Hutchings:

ABB13 is a great medium for inducing antibiotic production and has also been used to improve transformation efficiency in actinomycetes.

ABB1 base medium contained the following components (g/L):
*10g Bacto Soytone,
*9g yeast extract,
*10g soluble starch,
*15g cerelose
*Filter sterilised 3-N-morpholinopropanesulfonic acid (MOPS) buffer, pH 7.0, was added to ABB1 base medium after autoclaving to a final concentration of 50 mM.

References

Fitzgerals et al (1998). Sonication-Dependent Electroporation of the Erythromycin-Producing Bacterium Saccharopolyspora erythraea. AEM 674:1580–1583. https://journals.asm.org/doi/10.1128/aem.64.4.1580-1583.1998

ABB13

2026-01-27T12:39:13Z

Matt Hutchings: Created page with "ABB13 is a great medium for inducing antibiotic production and has also been used to improve transformation efficiency in actinomycetes. ABB1 base medium contained the following components (g/L): 10g Bacto Soytone, 9g yeast extract, 10g soluble starch, 15g cerelose Filter sterilised 3-N-morpholinopropanesulfonic acid (MOPS) buffer, pH 7.0, was added to ABB1 base medium after autoclaving to a final concentration of 50 mM. References Fitzgerals et al..."

ABB13 is a great medium for inducing antibiotic production and has also been used to improve transformation efficiency in actinomycetes.

ABB1 base medium contained the following components (g/L):
10g Bacto Soytone,
9g yeast extract,
10g soluble starch,
15g cerelose
Filter sterilised 3-N-morpholinopropanesulfonic acid (MOPS) buffer, pH 7.0, was added to ABB1 base medium after autoclaving to a final concentration of 50 mM.

References

Fitzgerals et al (1998). Sonication-Dependent Electroporation of the Erythromycin-Producing Bacterium Saccharopolyspora erythraea. AEM 674:1580–1583. https://journals.asm.org/doi/10.1128/aem.64.4.1580-1583.1998

Media recipes

2026-01-27T12:32:45Z

Matt Hutchings: /* Media Recipes */

{{DISPLAYTITLE:Media Recipes}}

[[File:Actinobase-icons-trans_Media-recipes.png|150px|Media Recipes|link=Media_recipes]]

=Media Recipes=

[[ABB13]]

[[A1]]

[[CM]] (Complete medium)

[[DNA]] (Difco Nutrient Agar) | [[DNB]] (Difco Nutrient Broth) | [[SNA]] (Soft Nutrient Agar)

[[Emerson medium]]

[[Gause's No. 1]]

[[GYM]] (Glucose + Yeast Extract + Malt Extract)

[[HT]] (Hickey-Tresner)

[[HV]]

[[IMA]] (Smash)

[[LB]] (Lysogeny Broth) | [[Lennox]] (Lennox agar) | [[Luria]] (Luria agar)

[[MC agar]] (Milk Casein Agar)

[[MM]] (Minimal medium)

[[SFM | MS]] (Mannitol Soya agar - a.k.a. Soya Flour Mannitol (SFM))

[[1/2 MS0 | 1/2 MS-S]]

[[1/2 MS+B5]]

[[MYM]] (Maltose + Yeast Extract + Malt Extract)

[[NMMP]] (minimal liquid medium)

[[OT]] (oligotrophic agar)

[[PDA]] (Potato Dextrose Agar)

[[R2]] | [[R2YE]] | [[R5]]

[[SC]] (Starch Casein agar)

[[SFM]] (Soya Flour Mannitol - a.k.a. Mannitol Soya agar)

[[SMM]] (supplemented liquid minimal medium) | [[SMMS]] (supplemented minimal medium, solid)

[[SNA]] (Soft nutrient agar)

[[SPY]] (Salt Peptone Yeast)

[[TSB]] (Tryptone Soy Broth)

[[YEME]] (Yeast Extract + Malt Extract)

[[YMPD]] (Yeast Meat Peptone Dextrose)

[[YMS]] (Yeast-Malt extract-Soluble starch medium)

[[YP]] (Yeast Peptone) | [[YPD]] (Yeast Peptone Dextrose)

[[2X YT]]

====International ''Streptomyces'' Project Media (ISP's)====

[[ISP2]]

[[ISP3]]

[[ISP4]]

[[ISP5]]

[[ISP6]]

[[ISP7]]

==Media Supplements==

[[Antibiotic stocks and working concentrations]]

[[Indicator compounds]]

[[Media supplements for activating cryptic biosynthetic gene clusters]]

[[Media supplements for induction gene expression from inducible promoters]]

[[Trace element solution]]

PSS170

2025-12-04T10:46:21Z

Matt Hutchings:

{{DISPLAYTITLE: pSS170}} (aka pIJ10770)

==Use==

pSS170 (=pIJ10770) Derivative of pMS82 which lacks the apramycin promoter fragment and contains a multiple cloning site (HygR)

==Features==
''Streptomyces'' phage phiBT1 integration site. Hygromycin resistance gene, oriT, ColEi origin of replication

==History==
Derived from pMS82 - https://www.addgene.org/68110/

==Map==

==Sequence links==

==References==
pMS82 background vector - Gregory, M.A., Till, R. and Smith, M.C., 2003. Integration site for Streptomyces phage φBT1 and development of site-specific integrating vectors. Journal of bacteriology, 185(17), pp.5320-5323.

Schlimpert S, Wasserstrom S, Chandra G, Bibb MJ, Findlay KC, Flärdh K, et al. Two dynamin-like proteins stabilize FtsZ rings during Streptomyces sporulation. Proc Natl Acad Sci. 2017 Jul 25;114(30):E6176–83.

PSS170

2025-12-04T10:45:42Z

Matt Hutchings:

{{DISPLAYTITLE: pSS170}} (aka pIJ10770)

==Use==
pSS170 is used for introducing genes to ''Streptomyces''. Because this plasmid is lacking a promoter upstream of the multiple cloning site (MCS), the gene of interest is often cloned in with its native promoter, in order to complement a knockout mutant

==Features==
''Streptomyces'' phage phiBT1 integration site. Hygromycin resistance gene, oriT, ColEi origin of replication

==History==
Derived from pMS82 - https://www.addgene.org/68110/

==Map==

==Sequence links==

==References==
pMS82 background vector - Gregory, M.A., Till, R. and Smith, M.C., 2003. Integration site for Streptomyces phage φBT1 and development of site-specific integrating vectors. Journal of bacteriology, 185(17), pp.5320-5323.

Schlimpert S, Wasserstrom S, Chandra G, Bibb MJ, Findlay KC, Flärdh K, et al. Two dynamin-like proteins stabilize FtsZ rings during Streptomyces sporulation. Proc Natl Acad Sci. 2017 Jul 25;114(30):E6176–83.

YMS

2025-11-04T12:03:11Z

Matt Hutchings:

Yeast extract: 4.0 g
Malt extract: 10.0 g
Soluble starch: 4.0 g (or sometimes as high as 30.0 g)
Agar: 20.0 g (for solid medium)
Calcium carbonate: 2.0 g (often added after adjusting pH)
Distilled water: 1000 mL

YMS

2025-11-04T12:01:06Z

Matt Hutchings: Created page with "Yeast extract: 4.0 g Malt extract: 10.0 g Soluble starch: 10.0 g (or sometimes as high as 30.0 g) Agar: 20.0 g (for solid medium) Calcium carbonate: 2.0 g (often added after adjusting pH) Distilled water: 1000 mL "

Yeast extract: 4.0 g
Malt extract: 10.0 g
Soluble starch: 10.0 g (or sometimes as high as 30.0 g)
Agar: 20.0 g (for solid medium)
Calcium carbonate: 2.0 g (often added after adjusting pH)
Distilled water: 1000 mL

Media recipes

2025-11-04T12:00:15Z

Matt Hutchings: /* Media Recipes */

{{DISPLAYTITLE:Media Recipes}}

[[File:Actinobase-icons-trans_Media-recipes.png|150px|Media Recipes|link=Media_recipes]]

=Media Recipes=

[[A1]]

[[CM]] (Complete medium)

[[DNA]] (Difco Nutrient Agar) | [[DNB]] (Difco Nutrient Broth) | [[SNA]] (Soft Nutrient Agar)

[[Emerson medium]]

[[Gause's No. 1]]

[[GYM]] (Glucose + Yeast Extract + Malt Extract)

[[HT]] (Hickey-Tresner)

[[HV]]

[[IMA]] (Smash)

[[LB]] (Lysogeny Broth) | [[Lennox]] (Lennox agar) | [[Luria]] (Luria agar)

[[MC agar]] (Milk Casein Agar)

[[MM]] (Minimal medium)

[[SFM | MS]] (Mannitol Soya agar - a.k.a. Soya Flour Mannitol (SFM))

[[1/2 MS0 | 1/2 MS-S]]

[[1/2 MS+B5]]

[[MYM]] (Maltose + Yeast Extract + Malt Extract)

[[NMMP]] (minimal liquid medium)

[[OT]] (oligotrophic agar)

[[PDA]] (Potato Dextrose Agar)

[[R2]] | [[R2YE]] | [[R5]]

[[SC]] (Starch Casein agar)

[[SFM]] (Soya Flour Mannitol - a.k.a. Mannitol Soya agar)

[[SMM]] (supplemented liquid minimal medium) | [[SMMS]] (supplemented minimal medium, solid)

[[SNA]] (Soft nutrient agar)

[[SPY]] (Salt Peptone Yeast)

[[TSB]] (Tryptone Soy Broth)

[[YEME]] (Yeast Extract + Malt Extract)

[[YMPD]] (Yeast Meat Peptone Dextrose)

[[YMS]] (Yeast-Malt extract-Soluble starch medium)

[[YP]] (Yeast Peptone) | [[YPD]] (Yeast Peptone Dextrose)

[[2X YT]]

====International ''Streptomyces'' Project Media (ISP's)====

[[ISP2]]

[[ISP3]]

[[ISP4]]

[[ISP5]]

[[ISP6]]

[[ISP7]]

==Media Supplements==

[[Antibiotic stocks and working concentrations]]

[[Indicator compounds]]

[[Media supplements for activating cryptic biosynthetic gene clusters]]

[[Media supplements for induction gene expression from inducible promoters]]

[[Trace element solution]]

ISP4

2025-10-16T13:23:12Z

Matt Hutchings: /* Preparation */

=ISP 4=
International Streptomyces Project medium 4, also named inorganic salts-starch agar

==Preparation==
*10 g Soluble Starch
*1g K2HPO4 (anhydrous)
*1 g MgSO4 x 7H2O
*1 g NaCl
*2 g (NH4)2SO4
*2 g CaCO3
*1 L dH2
*1 mL Trace Salts solution
**0.1 g FeSO4 x 7H2O
**0.1 g MnCl2 x 4H2O
**0.1 g ZnSO4 x 7H2O
**100 mL dH2O
*20 g agar

'''Instructions'''

Prepare as two separate solutions and mix before adding agar and autoclaving

===Solution 1===
#Make a paste of the soluble starch with a small volume of cold dH2O
#Bring the paste up to a volume of 500 mL
===Solution 2===
#Dissolve the remaining constituents and trace salts solution in 500 mL dH2O
#Adjust the pH so that it's between 7 and 7.4

==Uses==
*Screening for antibiotic production

==Notes==

==References==

The ActinoBase e-Seminar

2024-11-29T09:54:47Z

Matt Hutchings:

These seminars were broadcast during the Covid-19 pandemic when the world was locked down. They can be viewed on our YouTube channel.

==Previous Talks==
2021 Talks

20.10.21

Available at: https://www.youtube.com/watch?v=9QPadull3m4

*Dr. Kai Blin, Group Leader, Denmark Technical University
"Everything you wanted to know about antiSMASH but were afraid to ask"

06.10.21

Available at: https://www.youtube.com/watch?v=WcLTzTh2SW4

*Dr. Agustina Undabarrena, PostDoc Universidad Técnica Federico Santa María

"Rhodococcus comparative genomics: NRPS distribution and connection to an orphan metabolite"

22.09.21

Available at: https://www.youtube.com/watch?v=g0BYT1elTvw&t=13s

*Dr. Paige Mandelare Ruiz, PostDoc Smithsonian Marine Station
"Isolating Coral-derived Actinobacteria as Probiotics for the Treatment of Stony Coral Tissue Loss-Diseased Florida Corals"

08.09.21

Available at: https://www.youtube.com/watch?v=pWa4sCJqQTA&t=3s

*Dr. Alejandra Prieto-Davo, Group Leader Universidad Nacional Autónoma de México
"The maya underworld as a source of natural products
"

25.08.21

*Buse Cinar, PhD student at The Leibniz University
"Phosphodiesterase-mediated local c-di-GMP-signalling mechanisms in Streptomyces developmental control"

11.08.21

*Dr. Pablo Cruz-Morales, Group Leader, Denmark Technical University
"Engineering Natural product biosynthesis to make rocket fuels and medicines"

27.07.21

Available at: https://www.youtube.com/watch?v=iSlrPdI4ohU

*Dr. Jana Shniete, PostDocs, Edge Hill University & Dr. Nelly Selem, Group Leader, Universidad Nacional Autonoma de Mexico
"ActDES – a curated Actinobacterial Database for Evolutionary Studies"

14.07.21

Available at: https://www.youtube.com/watch?v=_RysFm5EbCY&t=9s

*Molly Keith-Baker - PhD Student, Strathclyde University
"First things first: Primary metabolism in Streptomyces"

29.06.21

Available at: https://www.youtube.com/watch?v=Plp_mbvvfeU

*Dr. Bronwyn Kirby-MCCullough - Group Leader, University of the Western Cape
"Screening actinobacteria associated with indigenous South African plants for natural products and accessing their potential use as plant growth promoters"

15.06.21

Available at: https://www.youtube.com/watch?v=LgO_M3M4pW0&t=1s

*Oliver Hennrich - PhD Student, Leibniz Institute DSMZ, Germany
"Generation of novel pristinamycin derivatives by mutasynthesis approaches"

2020 Talks

20.08.20

Available at: https://www.youtube.com/watch?v=LLBH3uJlQWc

*Eleanor Terrelonge, PhD Student, University of the West Indies, Jamaica
"Exploring Jamaican Actinomycetes: new weapons against antibiotic resistance"

06.08.20

*Dr. Beatriz Camara, Group Leader, Universidad Técnica Federico Santa María, Chile
"A genomics and metabolomics guided bioprospection of marine actinomycetes from the South Pacific"

23.07.20

Available at: https://www.youtube.com/watch?v=xZUKfIat-Uc

*Satria Kautsar, PhD Student, Wageningen University, Netherlands
"Mapping the secondary metabolic diversity of 209211 microbial genomes and MAGs (introducing BiG-SLiCE, a new, ultra-scalable BGC clustering tool)"

09.07.20

Available at: https://youtu.be/NUyXiq_xcsM

*Prof Yvonne Mast - Group Leader, Leibniz Institute DSMZ, Germany
"SARP-Driven activation of antibiotic gene clusters"

25.06.20 

Available at: https://youtu.be/IsyfayocPkI

*Prof Justin Nodwell + ECR Flash talks
"The ActinoBase ISBA seminar"

18.06.20

Available at: https://youtu.be/Fsd-69koBas

*Dr Virginia Helena Abarracin - Group Leader, National University of Tucuman, Argentina
"UV-resistant Actinobacteria from high-altitude Andean lakes: diversity and molecular mechanisms"

11.06.20

Available at: https://youtu.be/LhBMoiefrns

*Dr Bernhard Kepplinger - PostDoc, University of Newcastle, UK
"The Three Horsemen of the Amycolatopsis"

*Patrick Capel- PhD Student, University of Warwick, UK
"Cell-Free Transcription Translation for Natural Product Research"

04.06.20

Available at: https://www.youtube.com/watch?v=BMGYtd0gYLk

*Audam Chhun - PhD Student, University of Warwick, UK
"Phytoplankton trigger the production of cryptic metabolites in the marine Actinobacteria Salinispora tropica"

*Jonathan Parra - PhD Student, University of Strathclyde, UK
"Integrated multi-omics insights into the specialised metabolism of Pseudonocardia spp. isolated from the Southern Ocean"

28.05.20

Available at: https://youtu.be/cecBKLoonu4

*Dr Yaojun Tong - PostDoc at The Technical University of Denmark, Denmark
"Natural Products meet CRISPR, the BEST is coming"

*Emily Addington - PhD Student, University of Strathclyde, UK
"Disruption of the mce operon from Streptomyces affects spore resistance and results in precocious germination"

21.05.20

Available at: https://www.youtube.com/watch?v=Cc6eKGZQ1Qw

*Dr Scott Jarmusch - PostDoc at Uppsala University, Sweden
"Siderophore Wars: iron-mediated fungal starvation by extremotolerant Streptomyces sp. S29 desferrioxamine production"

*Dr Magalena Kotowska - PostDoc, Hirzfeld Institute of Immunology and Experimental Therapy, Poland
"A zinc binding GntR-like protein HypR controlling expression of genes associated with collagen and L-hydroxyproline utilisation in Streptomyces coelicolor A3(2)"

14.05.20

Available at:https://youtu.be/2swuBetcmSI

*Rebecca McHugh - PostDoc at the University of Strathclyde
"Aurodox: Teaching and old drug new tricks"

*Jan Falguera - PhD Student at the University of Toronto, Canada
"The effect of DNA damage on cell division in the filamentous bacterium Streptomyces"

07.05.20

'''Available at:''' https://www.youtube.com/watch?v=pYSfLwdskpg

*Dr Wilaiwan Koomsiri - PostDoc at Kasetsart University
“''''' Discovery of New Linear Lipopeptides from Streptomyces sp. KO-7888 by Activation of a SARP Regulatory Gene of a Cryptic NRPS Gene Cluster.'''''”

*Anne van der Meij - PhD student at Leiden University
“'''''Impact of plant hormones on growth and development of Actinobacteria.'''''”

30.04.20

'''Available at:''' https://youtu.be/5UekgN_-2Vo

*Dr Rebecca Devine - PostDoc at the University of East Anglia
“'''''From ants to antibiotics: Discovering novel medicines in the genomic era.'''''”

*David Mark - PhD student at the University of Strathclyde
“'''''The anti-pseudomonad activity of Micromonospora Species from the Atacama Desert.'''''”

==Organising committee==

The ActinoBase e-Seminar series is organised and hosted by a team of early-career Actinobacteria researchers, if you have any questions about the seminars then feel free to contact any of the members below:

The ActinoBase team: teamwikimyces@gmail.com

2021 committee

Agustina Undabarrena - Postdoctoral Researcher, Universidad Técnica Federico Santa María: agus.undabarrena@gmail.com

Katie Noble - PhD Student, John Innes Centre: katie.noble@jic.ac.uk

Lubabalo Ndidi - MSc Student, University of the Western Cape: lubabalo.ndidi.93@gmail.com

Molly Keith-Baker - PhD Student, University of Strathclyde: molly.keith-baker@strath.ac.uk

Oliver Hennrich - PhD Student, University of Tübingen/Leibniz Institute,DSMZ: oliver.hennrich@uni-tuebingen.de

2020 committee

Sam Prudence - PhD Student, University of East Anglia: s.prudence@uea.ac.uk
Twitter: [https://twitter.com/Sam_Prudence @Sam_Prudence]

David Mark - PhD Student, University of Strathclyde: david.mark@strath.ac.uk
Twitter: [https://twitter.com/DavidRcoMark @DavidRcoMark]

Alicia Russell - PhD Student, John Innes Centre: alicia.russell@jic.ac.uk
Twitter: [https://twitter.com/alisia_russell @alisia_russell]

Tom Mclean - PhD Student, University of East Anglia: t.mclean@uea.ac.uk
Twitter: [https://twitter.com/TomMcLean05 @TomMcLean05]

Emily Addington - PhD Student, University of Strathclyde

===Rules & Etiquette===
*Abusive behaviour within the live chat feed on YouTube will not be tolerated and will result in a permanent ban.
*Question for speakers can be submitted to moderators via the YouTube live chat or Twitter using the HashTag associated with each session.
*Individuals in the Zoom call should have their microphone muted and video switched off to minimize disruptions & save oin bandwidth.

The ActinoBase e-Seminar

2024-11-29T09:54:32Z

Matt Hutchings:

These seminars were broadcast during the Covid-19 pandemic when the world was locked down. they can be viewed on our YouTube channel.

==Previous Talks==
2021 Talks

20.10.21

Available at: https://www.youtube.com/watch?v=9QPadull3m4

*Dr. Kai Blin, Group Leader, Denmark Technical University
"Everything you wanted to know about antiSMASH but were afraid to ask"

06.10.21

Available at: https://www.youtube.com/watch?v=WcLTzTh2SW4

*Dr. Agustina Undabarrena, PostDoc Universidad Técnica Federico Santa María

"Rhodococcus comparative genomics: NRPS distribution and connection to an orphan metabolite"

22.09.21

Available at: https://www.youtube.com/watch?v=g0BYT1elTvw&t=13s

*Dr. Paige Mandelare Ruiz, PostDoc Smithsonian Marine Station
"Isolating Coral-derived Actinobacteria as Probiotics for the Treatment of Stony Coral Tissue Loss-Diseased Florida Corals"

08.09.21

Available at: https://www.youtube.com/watch?v=pWa4sCJqQTA&t=3s

*Dr. Alejandra Prieto-Davo, Group Leader Universidad Nacional Autónoma de México
"The maya underworld as a source of natural products
"

25.08.21

*Buse Cinar, PhD student at The Leibniz University
"Phosphodiesterase-mediated local c-di-GMP-signalling mechanisms in Streptomyces developmental control"

11.08.21

*Dr. Pablo Cruz-Morales, Group Leader, Denmark Technical University
"Engineering Natural product biosynthesis to make rocket fuels and medicines"

27.07.21

Available at: https://www.youtube.com/watch?v=iSlrPdI4ohU

*Dr. Jana Shniete, PostDocs, Edge Hill University & Dr. Nelly Selem, Group Leader, Universidad Nacional Autonoma de Mexico
"ActDES – a curated Actinobacterial Database for Evolutionary Studies"

14.07.21

Available at: https://www.youtube.com/watch?v=_RysFm5EbCY&t=9s

*Molly Keith-Baker - PhD Student, Strathclyde University
"First things first: Primary metabolism in Streptomyces"

29.06.21

Available at: https://www.youtube.com/watch?v=Plp_mbvvfeU

*Dr. Bronwyn Kirby-MCCullough - Group Leader, University of the Western Cape
"Screening actinobacteria associated with indigenous South African plants for natural products and accessing their potential use as plant growth promoters"

15.06.21

Available at: https://www.youtube.com/watch?v=LgO_M3M4pW0&t=1s

*Oliver Hennrich - PhD Student, Leibniz Institute DSMZ, Germany
"Generation of novel pristinamycin derivatives by mutasynthesis approaches"

2020 Talks

20.08.20

Available at: https://www.youtube.com/watch?v=LLBH3uJlQWc

*Eleanor Terrelonge, PhD Student, University of the West Indies, Jamaica
"Exploring Jamaican Actinomycetes: new weapons against antibiotic resistance"

06.08.20

*Dr. Beatriz Camara, Group Leader, Universidad Técnica Federico Santa María, Chile
"A genomics and metabolomics guided bioprospection of marine actinomycetes from the South Pacific"

23.07.20

Available at: https://www.youtube.com/watch?v=xZUKfIat-Uc

*Satria Kautsar, PhD Student, Wageningen University, Netherlands
"Mapping the secondary metabolic diversity of 209211 microbial genomes and MAGs (introducing BiG-SLiCE, a new, ultra-scalable BGC clustering tool)"

09.07.20

Available at: https://youtu.be/NUyXiq_xcsM

*Prof Yvonne Mast - Group Leader, Leibniz Institute DSMZ, Germany
"SARP-Driven activation of antibiotic gene clusters"

25.06.20 

Available at: https://youtu.be/IsyfayocPkI

*Prof Justin Nodwell + ECR Flash talks
"The ActinoBase ISBA seminar"

18.06.20

Available at: https://youtu.be/Fsd-69koBas

*Dr Virginia Helena Abarracin - Group Leader, National University of Tucuman, Argentina
"UV-resistant Actinobacteria from high-altitude Andean lakes: diversity and molecular mechanisms"

11.06.20

Available at: https://youtu.be/LhBMoiefrns

*Dr Bernhard Kepplinger - PostDoc, University of Newcastle, UK
"The Three Horsemen of the Amycolatopsis"

*Patrick Capel- PhD Student, University of Warwick, UK
"Cell-Free Transcription Translation for Natural Product Research"

04.06.20

Available at: https://www.youtube.com/watch?v=BMGYtd0gYLk

*Audam Chhun - PhD Student, University of Warwick, UK
"Phytoplankton trigger the production of cryptic metabolites in the marine Actinobacteria Salinispora tropica"

*Jonathan Parra - PhD Student, University of Strathclyde, UK
"Integrated multi-omics insights into the specialised metabolism of Pseudonocardia spp. isolated from the Southern Ocean"

28.05.20

Available at: https://youtu.be/cecBKLoonu4

*Dr Yaojun Tong - PostDoc at The Technical University of Denmark, Denmark
"Natural Products meet CRISPR, the BEST is coming"

*Emily Addington - PhD Student, University of Strathclyde, UK
"Disruption of the mce operon from Streptomyces affects spore resistance and results in precocious germination"

21.05.20

Available at: https://www.youtube.com/watch?v=Cc6eKGZQ1Qw

*Dr Scott Jarmusch - PostDoc at Uppsala University, Sweden
"Siderophore Wars: iron-mediated fungal starvation by extremotolerant Streptomyces sp. S29 desferrioxamine production"

*Dr Magalena Kotowska - PostDoc, Hirzfeld Institute of Immunology and Experimental Therapy, Poland
"A zinc binding GntR-like protein HypR controlling expression of genes associated with collagen and L-hydroxyproline utilisation in Streptomyces coelicolor A3(2)"

14.05.20

Available at:https://youtu.be/2swuBetcmSI

*Rebecca McHugh - PostDoc at the University of Strathclyde
"Aurodox: Teaching and old drug new tricks"

*Jan Falguera - PhD Student at the University of Toronto, Canada
"The effect of DNA damage on cell division in the filamentous bacterium Streptomyces"

07.05.20

'''Available at:''' https://www.youtube.com/watch?v=pYSfLwdskpg

*Dr Wilaiwan Koomsiri - PostDoc at Kasetsart University
“''''' Discovery of New Linear Lipopeptides from Streptomyces sp. KO-7888 by Activation of a SARP Regulatory Gene of a Cryptic NRPS Gene Cluster.'''''”

*Anne van der Meij - PhD student at Leiden University
“'''''Impact of plant hormones on growth and development of Actinobacteria.'''''”

30.04.20

'''Available at:''' https://youtu.be/5UekgN_-2Vo

*Dr Rebecca Devine - PostDoc at the University of East Anglia
“'''''From ants to antibiotics: Discovering novel medicines in the genomic era.'''''”

*David Mark - PhD student at the University of Strathclyde
“'''''The anti-pseudomonad activity of Micromonospora Species from the Atacama Desert.'''''”

==Organising committee==

The ActinoBase e-Seminar series is organised and hosted by a team of early-career Actinobacteria researchers, if you have any questions about the seminars then feel free to contact any of the members below:

The ActinoBase team: teamwikimyces@gmail.com

2021 committee

Agustina Undabarrena - Postdoctoral Researcher, Universidad Técnica Federico Santa María: agus.undabarrena@gmail.com

Katie Noble - PhD Student, John Innes Centre: katie.noble@jic.ac.uk

Lubabalo Ndidi - MSc Student, University of the Western Cape: lubabalo.ndidi.93@gmail.com

Molly Keith-Baker - PhD Student, University of Strathclyde: molly.keith-baker@strath.ac.uk

Oliver Hennrich - PhD Student, University of Tübingen/Leibniz Institute,DSMZ: oliver.hennrich@uni-tuebingen.de

2020 committee

Sam Prudence - PhD Student, University of East Anglia: s.prudence@uea.ac.uk
Twitter: [https://twitter.com/Sam_Prudence @Sam_Prudence]

David Mark - PhD Student, University of Strathclyde: david.mark@strath.ac.uk
Twitter: [https://twitter.com/DavidRcoMark @DavidRcoMark]

Alicia Russell - PhD Student, John Innes Centre: alicia.russell@jic.ac.uk
Twitter: [https://twitter.com/alisia_russell @alisia_russell]

Tom Mclean - PhD Student, University of East Anglia: t.mclean@uea.ac.uk
Twitter: [https://twitter.com/TomMcLean05 @TomMcLean05]

Emily Addington - PhD Student, University of Strathclyde

===Rules & Etiquette===
*Abusive behaviour within the live chat feed on YouTube will not be tolerated and will result in a permanent ban.
*Question for speakers can be submitted to moderators via the YouTube live chat or Twitter using the HashTag associated with each session.
*Individuals in the Zoom call should have their microphone muted and video switched off to minimize disruptions & save oin bandwidth.

PIJ86

2024-11-29T09:46:55Z

Matt Hutchings:

pIJ86 is a multicopy expression vector with the high-level constitutive ''ermE*'' promoter. The backbone carries apramycin resistance. It can be transferred from ''E. coli'' to ''Streptomyces'' species by conjugation.

[https://www.ncbi.nlm.nih.gov/nuccore/2831519705 Complete sequence of pIJ86]

[[File:pIJ86.jpg|pIJ86 map]]

PIJ86

2024-11-29T09:46:31Z

Matt Hutchings:

File:PIJ86.jpg

2024-11-29T09:41:55Z

Matt Hutchings:

PIJ86

2024-11-29T09:41:38Z

Matt Hutchings:

PIJ86

2024-11-28T10:18:49Z

Matt Hutchings:

PIJ86

2024-11-28T10:18:33Z

Matt Hutchings:

pIJ86 is a multicopy expression vector with the high-level constitutive ''ermE*'' promoter. The backbone carries apramycin resistance. It can be transferred from E. coli to ''Streptomyces'' species by conjugation.

[https://www.ncbi.nlm.nih.gov/nuccore/2831519705 Complete sequence of pIJ86]

PIJ86

2024-11-28T10:17:33Z

Matt Hutchings:

pIJ86 is a multicopy expression vector with the constitutive *ermE** promoter. The backbone carries apramycin resistance.

[https://www.ncbi.nlm.nih.gov/nuccore/2831519705 Complete sequence of pIJ86]

PIJ86

2024-11-28T10:15:57Z

Matt Hutchings:

[https://www.ncbi.nlm.nih.gov/nuccore/2831519705 Complete sequence of pIJ86]

PIJ86

2024-11-28T10:15:40Z

Matt Hutchings:

Plasmid sequence of pIJ86 is here [https://www.ncbi.nlm.nih.gov/nuccore/2831519705 here]