https://actinobase.org/api.php?action=feedcontributions&feedformat=atom&user=RTEvans ActinoBase - User contributions [en-gb] 2026-10-17T19:25:39Z User contributions MediaWiki 1.39.3 https://actinobase.org/index.php?title=RRNA_depletion_with_Streptomyces_coelicolor_A3(2)_specific_probes&diff=2199 RRNA depletion with Streptomyces coelicolor A3(2) specific probes 2023-11-01T17:00:14Z <p>RTEvans: /* Notes */</p> <hr /> <div>==Organisms==<br /> <br /> This protocol has been tested with &lt;em&gt;[[Streptomyces coelicolor]]&lt;/em&gt; A3(2) MT1110<br /> <br /> ==Description==<br /> <br /> rRNA depletion using commercial kits targeting generic bacterial sequences is not 100% efficient in the High GC organism &lt;em&gt;S. coelicolor&lt;/em&gt;. <br /> <br /> Depletion using the NEBNext RNA Depletion Core Reagent Set with RNA Sample Purification Beads (https://www.neb.com/en-gb/products/e7870-nebnext-rna-depletion-core-reagent-set-with-rna-sample-purification-beads#Product%20Information) and custom DNA probes designed for &lt;em&gt;S. coelicolor&lt;/em&gt; is more effective than the QIAseq FastSelect 5S/16S/23S kit (Qiagen #335927); NEBNext rRNA Depletion Kit (Bacteria) (NEB #E7850); and Illumina Ribo-zero rRNA Removal Kit (Bacteria) (Illumina #MRZMB12424, discontinued). The Illumina kit tested was subsequently replaced by the Illumina Ribo-Zero Plus rRNA Depletion Kit (Illumina #20040526). <br /> <br /> In addition, three abundant non-mRNA RNAs can also be depleted using the custom probes (Table 1).<br /> <br /> '''Table 1.''' Abundant non-mRNA RNAs in &lt;em&gt;S. coelicolor&lt;/em&gt;.<br /> <br /> [[File: rRNA_depletion_table_1.png]]<br /> <br /> ==Materials==<br /> <br /> Single stranded DNA probes at 2µM concentration in IDTE pH 7.5 (10mM Tris-HCl / 0.1mM EDTA). Integrated DNA Technologies can produce these from their 25nmole DNA oligo pool product. Probes are listed in Table 2 in the Notes section below.<br /> <br /> NEBNext RNA Depletion Core Reagent Set with RNA Sample Purification Beads (https://www.neb.com/en-gb/products/e7870-nebnext-rna-depletion-core-reagent-set-with-rna-sample-purification-beads#Product%20Information).<br /> <br /> ==Protocols==<br /> <br /> As per manufacturer’s instructions.<br /> <br /> ==Notes==<br /> <br /> The following histograms illustrate effectiveness of rRNA depletion using various methods. Histograms show percentage of bases aligned to NCBI reference sequence NC_003888.3 using Hisat2 program for a number of samples, classified as follows - Coding = RNA aligned to coding sequences; Transcribed not translated = RNA aligned to rRNA, tRNA and sRNA; Intergenic = RNA aligned to 5' or 3' UTRs or between annotated sequences. Each graph is from a separate experiment, and total RNA has been extracted from mycelium using chloroform / phenol before being rRNA depleted, libraries prepared and sequenced.<br /> <br /> '''QIAseq FastSelect 5S/16S/23S kit'''<br /> <br /> [[File: PanelA.png]] <br /> <br /> '''NEBNext rRNA Depletion Kit (Bacteria)'''<br /> <br /> [[File: PanelB.png]]<br /> <br /> '''Illumina Ribo-zero rRNA Removal Kit (Bacteria)'''<br /> <br /> [[File: PanelC.png]] <br /> <br /> '''rRNA depletion with &lt;em&gt;S. coelicolor&lt;/em&gt; specific probes<br /> <br /> [[File: PanelD.png]]<br /> <br /> <br /> '''Table 2''' List of probes<br /> <br /> {| class=&quot;wikitable&quot;<br /> !Sequence<br /> !Target<br /> |-<br /> |CAAGTCCTTGCGCGACGCTCCCACGGCTTGTAGGCACACGGTTTCAGGTACTATTT<br /> |rRNA<br /> |-<br /> |CAGTCACGACGCAAGGACAAGTCCTTGCGCGACGCTCCCAC<br /> |rRNA<br /> |-<br /> |ACCTCGCAACACACCGCAAACTCGCAGGCTCATTCTTCAAAAGGCACGCAGTCACGACG<br /> |rRNA<br /> |-<br /> |CTACGGCTTCCCCACCCGGGTTAACCTCGCAACACACCGC<br /> |rRNA<br /> |-<br /> |TTAACCCAACATCTCACGACACGAGCTGACGACAGCCATGCACCACCTGTACACCGA<br /> |rRNA<br /> |-<br /> |CGCTCGTTGCGGGACTTAACCCAACATCTCACGACACGAG<br /> |rRNA<br /> |-<br /> |CAACACCCCGAAGGGCTTGCTGGCAACACGGGACAAGGGTTG<br /> |rRNA<br /> |-<br /> |CACCTTCCTCCGAGTTGACCCCGGCGGTCTCCCGTGAGTC<br /> |rRNA<br /> |-<br /> |GACATAAGGGGCATGATGACTTGACGTCGTCCCCACCTTC<br /> |rRNA<br /> |-<br /> |GTATCGCAGCTCATTGTACCGGCCATTGTAGCACGTGTGCAGCCCAAGACATAA<br /> |rRNA<br /> |-<br /> |TTTTGAGATTCGCTCCACCTTGCGGTATCGCAGCTCATTGTAC<br /> |rRNA<br /> |-<br /> |CAGACCCCAATCCGAACTGAGACCGGCTTTTTGAGATTCG<br /> |rRNA<br /> |-<br /> |AATGCTGATCTGCGATTACTAGCGACTCCGACTTCATGGGGTCGAGTTGCAGAC<br /> |rRNA<br /> |-<br /> |GAACGTATTCACCGCAGCAATGCTGATCTGCGATTACTAG<br /> |rRNA<br /> |-<br /> |TACCGACTTTCGTGACGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTC<br /> |rRNA<br /> |-<br /> |GCCACCGGCTTCGGGTGTTACCGACTTTCGTGACGTGACG<br /> |rRNA<br /> |-<br /> |TTCGTCCCAATCGCCAGTCCCACCTTCGACAGCTCCCTCCCACAAGGGGTTGGG<br /> |rRNA<br /> |-<br /> |CGCACCTTCCGGTACGGCTACCTTGTTACGACTTCGTCCCAATCGCCAGTCCCA<br /> |rRNA<br /> |-<br /> |TTCGACCCTCGCAGATGCCTGCGAGGGTCAGTATCCGGTATTAGACCCCGTTTCCAG<br /> |rRNA<br /> |-<br /> |GAAAGGAGGTGATCCAGCCGCACCTTCCGGTACGGCTACC<br /> |rRNA<br /> |-<br /> |TTCACCGCCGGAGCTTTCGACCCTCGCAGATGCCTGCGAG<br /> |rRNA<br /> |-<br /> |CATTACCTCACCAACAAGCTGATAGGCCGCGGGCTCATCCTTC<br /> |rRNA<br /> |-<br /> |CTCTCAGGCCGGCTACCCGTCGTCGCCTTGGTGAGCCATTAC<br /> |rRNA<br /> |-<br /> |GTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGGCCGGTCG<br /> |rRNA<br /> |-<br /> |TGCATCAGGCTTTCGCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCT<br /> |rRNA<br /> |-<br /> |TGCTGAAAGAGGTTTACAACCCGAAGGCCGTCATCCCTCACGCGGCGTCGCTGCATCA<br /> |rRNA<br /> |-<br /> |TTCTTCTGCAGGTACCGTCACTTTCGCTTCTTCCCTGCTGAAAGAGGTTTACAA<br /> |rRNA<br /> |-<br /> |CTACGTATTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTCTG<br /> |rRNA<br /> |-<br /> |CTCTTTACGCCCAATAATTCCGGACAACGCTTGCGCCCTACGTATTAC<br /> |rRNA<br /> |-<br /> |GGGCTTTCACAACCGACGTGACAAGCCGCCTACGAGCTCT<br /> |rRNA<br /> |-<br /> |CTACCGAACTCTAGCCTGCCCGTATCGACTGCAGACCCGGGGTTAAGCCCCG<br /> |rRNA<br /> |-<br /> |ATATCTGCGCATTTCACCGCTACACCAGGAATTCCGATCTCCCCTACCGAACTCTAG<br /> |rRNA<br /> |-<br /> |CGGTGTTCCTCCTGATATCTGCGCATTTCACCGCTACACC<br /> |rRNA<br /> |-<br /> |ACGCTTTCGCTCCTCAGCGTCAGTATCGGCCCAGAGATCCGCCTTCGCCACCGGTGT<br /> |rRNA<br /> |-<br /> |GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCG<br /> |rRNA<br /> |-<br /> |ACAACGTGGAATGTTGCCCACACCTAGTGCCCACCGTTTACGGCGTGGACTACCA<br /> |rRNA<br /> |-<br /> |CTTAATGCGTTAGCTGCGGCACGGACAACGTGGAATGTTG<br /> |rRNA<br /> |-<br /> |TCAATTCCTTTGAGTTTTAGCCTTGCGGCCGTACTCCCCAGGCGGGGCACTTAA<br /> |rRNA<br /> |-<br /> |ATTAAGCCACATGCTCCGCCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTTA<br /> |rRNA<br /> |-<br /> |TCAAGCCTTGGTAAGGTTCTTCGCGTTGCGTCGAATTAAGCCACATGCTCCGCCGCTT<br /> |rRNA<br /> |-<br /> |CATCTCTGATGCTTTCCGGTGTATGTCAAGCCTTGGTAAGGTTCTTC<br /> |rRNA<br /> |-<br /> |ACAGCCATGCACCACCTGTACACCGACCACAAGGGGGGCACCATCTCTGATGCTTTCC<br /> |rRNA<br /> |-<br /> |TGTAACCGGGCGTTTCCCCTACGCTATGACCACCGAAACC<br /> |rRNA<br /> |-<br /> |CAGTACCATCGGCGCTGTAAGGCTTAGCTTCCGGGTTCGAAATGTAAC<br /> |rRNA<br /> |-<br /> |GTTCGGCGGCGTCCTACTCTCCCACAGGGTCCCCCCTGCA<br /> |rRNA<br /> |-<br /> |CACTCCCCTCAACTCCGAAGAGATCAGGGCGGCTTCACGG<br /> |rRNA<br /> |-<br /> |CCATCACTCACCTACTAACCGCTTGGTTCGGCGGCTCCAC<br /> |rRNA<br /> |-<br /> |GTCCTTCATCGGTTCCTGGTGCCAAGGCATCCACCGTGCGCCCTTAAAAACTT<br /> |rRNA<br /> |-<br /> |CTTGTAGGCACACGGTTTCAGGTACTATTTCACTCCGCTCCCG<br /> |rRNA<br /> |-<br /> |CACGCAGTCACGAGACACCAAGCAAGCTTGATGTCCGACGCTCCCACGGCTTGTAGGCAC<br /> |rRNA<br /> |-<br /> |TCTTCAAAAGGCACGCAGTCACGAGACACCAAGCAAGCTT<br /> |rRNA<br /> |-<br /> |CACCCGGGTTAACCTCGCAACACACTGCAAACTCGCAGGC<br /> |rRNA<br /> |-<br /> |GCGTGCTACTAAAACGCCCTATTCGGACTCGCTTTCGCTACGGCTTCC<br /> |rRNA<br /> |-<br /> |GATCACTCCGCTTCGGGTCTTGAGCGTGCTACTAAAACGC<br /> |rRNA<br /> |-<br /> |TCGCAGATGCCTGCAAGGGTCAGTATCCGGTATTAGACCCCGTTT<br /> |rRNA<br /> |-<br /> |TCATCCTTCACCGCCGCAGCTTTCGAACCTCGCAGATGCC<br /> |rRNA<br /> |-<br /> |ATGTAACCGGGCGTTTCCCCTACGCTATGACCACCGAAAC<br /> |rRNA<br /> |-<br /> |CGTCCTACTCTCCCACAGGGTCCCCCCTGCAGTACCATCG<br /> |rRNA<br /> |-<br /> |CATCGCCTATCCAGTGCTCTACCTCCGGCAAGAAACACAC<br /> |rRNA<br /> |-<br /> |CATTCGGAGTTTGGCTAAGGTCAGTAACCCGGTAGGGCCCATCG<br /> |rRNA<br /> |-<br /> |CAGTCTCACTGCCGCGCTCTCACTTACCGGCATTCGGAGTTTG<br /> |rRNA<br /> |-<br /> |ATGCTCTGGGCTGTTTCCCTCTCGACCATGGAGCTTATCCCCCACAGTCTCACTG<br /> |rRNA<br /> |-<br /> |CTTAGGGGCCTTAGTCGATGCTCTGGGCTGTTTCCCTCTCG<br /> |rRNA<br /> |-<br /> |GTTGTCTGTGCGACTCCACATCCTTTCCCACTTAGCGTACGCTTAG<br /> |rRNA<br /> |-<br /> |TATTACGCACTCTTTCAAGGGTGGCTGCTTCTAAGCCAACCTCCTGGTTGTCTGT<br /> |rRNA<br /> |-<br /> |GATCAAAGCCTGGTTGACGACTCCCCGGGGCCTATCGTGG<br /> |rRNA<br /> |-<br /> |CGGAATCACTAGACCAGTGAGCTATTACGCACTCTTTCAAGG<br /> |rRNA<br /> |-<br /> |TATTGCTGCAATGACACGACTTCGGCGGTACGCTTGAGCCCCGCTACATTGTCG<br /> |rRNA<br /> |-<br /> |CATCACAGCCGGCGTTGGCCGTATTGCTGCAATGACACGACTTC<br /> |rRNA<br /> |-<br /> |TCCACAACTCGCTTCCGCGGCTGCTTCACCCGGCACACGACGCTCCCCTACCCATCACA<br /> |rRNA<br /> |-<br /> |TACTCATGCCTGCATTCTCACTCGTGAACCGTCCACAACTCGCTTCCGCGGCTGCTT<br /> |rRNA<br /> |-<br /> |CAAACGTTTCTCACGTTTGTATCGCTACTCATGCCTGCATTCTCACTC<br /> |rRNA<br /> |-<br /> |CAGGAACCCTTAGTCAATCGGCGCAAACGTTTCTCACGTTT<br /> |rRNA<br /> |-<br /> |CTTAGGTCCCGACTTACCCTGGGCAGATCAGCTTGACCCAG<br /> |rRNA<br /> |-<br /> |GAATATCAACCGGTTATCCATCGACTACGCCTGTCGGCCTCGCCTTAGGTC<br /> |rRNA<br /> |-<br /> |TTAGCATCACGATGCTCGATGTTTGACGCTTCACAGCGGGTACCGGAATATCAA<br /> |rRNA<br /> |-<br /> |GAAGAGATCAGGGCGGCTTCACGGCCTTAGCATCACGATG<br /> |rRNA<br /> |-<br /> |CATCACTCACCTACTAACCGCTTGGTTCGGCGGCTCCACC<br /> |rRNA<br /> |-<br /> |TGTGTTCAGCAGCGGGTGACAGCCCATGACGACTGCCGGGTTTCCCCATTCGGACACCC<br /> |rRNA<br /> |-<br /> |GACAACCACCGCCCGGGATGGACTACCTTCCTGCGTCACCCCAT<br /> |rRNA<br /> |-<br /> |CAGATGCTCGCGTCCACTGTGCAGTTCTCAAACAACGACC<br /> |rRNA<br /> |-<br /> |TGCGGATTTACCTACACACCGGCCTACACCCTTACCCCGGGACAACCACCG<br /> |rRNA<br /> |-<br /> |CATCAGGTCTCAGCCACAAGGTGTGCGGATTTACCTACAC<br /> |rRNA<br /> |-<br /> |TTTTCTCGACAGCATAGGATCATCCACTTCACCACAATCGGCTCGGCATCAGGTCT<br /> |rRNA<br /> |-<br /> |CCATGAAACTCGCTAGAGGCTTTTCTCGACAGCATAGGATCATC<br /> |rRNA<br /> |-<br /> |TATTCTCTACCTGACCACCTGAGTCGGTTTAGGGTACGGGCCGCCATGAAACT<br /> |rRNA<br /> |-<br /> |GAGTTCCTTAACCATAGTTCACCCGAACGCCTCGGTATTCTCTACCTGAC<br /> |rRNA<br /> |-<br /> |CTTCTCCCGAAGTTACGGGGGCATTTTGCCGAGTTCCTTAACCA<br /> |rRNA<br /> |-<br /> |CACCCCCAGCTCAGAGTGCAAAACTCGTCACCAGGTGTGG<br /> |rRNA<br /> |-<br /> |GTGTTTTTAGTAAACAGTCGCTTCTCGCTGGTCTCTGCGG<br /> |rRNA<br /> |-<br /> |GTTCCCTCCACACTGCCTATGTGTTCAGCAGCGGGTGACAG<br /> |rRNA<br /> |-<br /> |GTATACATCGCCTTACGGCTTCGCACGGACCTGTGTTTTTA<br /> |rRNA<br /> |-<br /> |CTTAACGTTCCAGCACCGGGCAGGCGTCAGTCCGTATACATC<br /> |rRNA<br /> |-<br /> |TAAGTTCTCAGCTTCGCCACACCGAAATGTGACTAACCGGTCCCCTTAACGTT<br /> |rRNA<br /> |-<br /> |TTAGGATGGTTATAGTTACCACCGCCGTTTACTGGCGCTTAAGTTCTCAG<br /> |rRNA<br /> |-<br /> |GTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGATGGTTATAGT<br /> |rRNA<br /> |-<br /> |TATGGTTGAGACAGTCGAGAAGTCGTTACGCCATTCGTGCAGGTCGGAACTTACC<br /> |rRNA<br /> |-<br /> |GAAACGAGCATCTTTACTCGTAGTGCAATTTCACCGGGCCTATGGTTGAG<br /> |rRNA<br /> |-<br /> |GGTCTTTCCGTCCTGCTGCGCGAAACGAGCATCTTTACTC<br /> |rRNA<br /> |-<br /> |GAACCGAACACCAATATCAAACTGTAGTAAAGGTCCCGGGGTCTTTC<br /> |rRNA<br /> |-<br /> |CTTCAAAGTCTCCCAGCTATCCTACACAAGCCGAACCGAACACCAATA<br /> |rRNA<br /> |-<br /> |AGACTGGTATTTCAACGACGACTCCACCCACACTGGCGTGCGAGCTTCAAAGTCT<br /> |rRNA<br /> |-<br /> |GTTAGACATCCAGCACGACCAGACTGGTATTTCAACGACGACTC<br /> |rRNA<br /> |-<br /> |TTAAACTACCCATCAGACACTGTCCCTGATCCGGATCACGGACCCAGGTTAGACAT<br /> |rRNA<br /> |-<br /> |GAATCACGGTTGTTTTCTCTTCCTGCGGGTACTGAGATGTTTCACTTC<br /> |rRNA<br /> |-<br /> |GTTACTCTTTAGGAGGCAACCGCCCCAGTTAAACTACCCATCAGACACTG<br /> |rRNA<br /> |-<br /> |ACTCAACACCTGATTGCCAACCAGGCTGAGGGAACCTTTGGGCGCCTCCGTTACT<br /> |rRNA<br /> |-<br /> |GTCTCACAGTCAAGCTCCCTTGTGCACTTACACTCAACACCTGATTG<br /> |rRNA<br /> |-<br /> |GACTTTCGTCCCTGCTCGACCCGTCGGTCTCACAGTCAAG<br /> |rRNA<br /> |-<br /> |TACCTTTTATCCGTTGAGCGACGGCGCTTCCACAAGCCACCGCCGGATCACTAGTC<br /> |rRNA<br /> |-<br /> |GTCGATATGGACTCTTGGGGAAGATCAGCCTGTTATCCCCGGGGTACCTTTTAT<br /> |rRNA<br /> |-<br /> |GACATCGAGGTGCCAAACCATCCCGTCGATATGGACTCTTG<br /> |rRNA<br /> |-<br /> |TTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTCCAGCCCCAGGATGCGACGA<br /> |rRNA<br /> |-<br /> |TCACGACGTTCTAAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACC<br /> |rRNA<br /> |-<br /> |GATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTC<br /> |rRNA<br /> |-<br /> |GTCCTCTCGTACTAGGGACAGCCCTTCTCAAGACTCCTAC<br /> |rRNA<br /> |-<br /> |TCTCACACGTATACGGTTTGGCCTCATCCGGTTTCGCTCGCCACTACTCCCGGAAT<br /> |rRNA<br /> |-<br /> |CATGCCCTTGGCAGGACAACTGGCACACCAGAGGTTCGTC<br /> |rRNA<br /> |-<br /> |CTTAGATGCTTTCAGCGGTTATCCCTCCCGAACGTAGCCAAC<br /> |rRNA<br /> |-<br /> |GTGGGAGTCCTCATCTCGAAGCAGGCTTCCCGCTTAGATG<br /> |rRNA<br /> |-<br /> |CTATCAACCCAGTCGTCTACTGGGAGCCTTAACCCCTCAAGG<br /> |rRNA<br /> |-<br /> |GCCTATTAGTACCGGTCACCTCCACACCTCACGGTGCTTCCAGATCCGGCCTATCAAC<br /> |rRNA<br /> |-<br /> |AGGACAAGCCCTCGGCCTATTAGTACCGGTCACCTCCACA<br /> |rRNA<br /> |-<br /> |CACACGCAACCCCTGCCGGGTCTCACACGTATACGGTTTG<br /> |rRNA<br /> |-<br /> |TTTCTGACTCGTCTCACGGCCGGCAGACCGTAAAAGAGAGATCCCACAACCCCGCACA<br /> |rRNA<br /> |-<br /> |TTTCGCATGTCCTTCGCCTACATCAACGGTTTCTGACTCGTCTCACGGCCGGCAGA<br /> |rRNA<br /> |-<br /> |CTTACCCTCTACGCCGGACCTTTCGCATGTCCTTCGCCTACATC<br /> |rRNA<br /> |-<br /> |GTGTCTCTCAAACGAGCCGTTGACGTTTCGACTACGGGGGTCTTACCCTCTA<br /> |rRNA<br /> |-<br /> |ATCCACCGTGCGCCCTTAAAAACTTGGCCACAGATGCTCG<br /> |rRNA<br /> |-<br /> |GATTTCTCGGGCCCCGTGCTACTTGGGTGTCTCTCAAACGAG<br /> |rRNA<br /> |-<br /> |GAATATTTAGGCTTAGCGGGTGGTCCCGCCAGATTCACACGGGATTTCTC<br /> |rRNA<br /> |-<br /> |CTCACGGTACTATCCGCTATCGGTCACCAGGGAATATTTAGGCTTAG<br /> |rRNA<br /> |-<br /> |GTTTCAGGTACTATTTCACTCCGCTCCCGCGGTACTTTTCACCATTC<br /> |rRNA<br /> |-<br /> |GATGGAGCAAGCTCCATCCCGACGCTCCCACGGCTTGTAGGCACACGGTTTCAGGTACT<br /> |rRNA<br /> |-<br /> |CACGCAGTCACGAGGATGGAGCAAGCTCCATCCCGACGCTC<br /> |rRNA<br /> |-<br /> |CTCGGGTTAACCTCGCAACACACCGCAAACTCGCAGGCTCATTCTTCAAAAGGCACGC<br /> |rRNA<br /> |-<br /> |CTACGGCTTCCCCACTCGGGTTAACCTCGCAACACACCGC<br /> |rRNA<br /> |-<br /> |GTCTTGAGCGTGCTACTAAAAACGCCCTATTCGGACTCGC<br /> |rRNA<br /> |-<br /> |CTCCCACGTCCTTCATCGGTTCCTGGTGCCAAGGCATCCAC<br /> |rRNA<br /> |-<br /> |CTTCAACCTGCCCATGGCTAGATCACTCCGCTTCGGGTCTTGAG<br /> |rRNA<br /> |-<br /> |GGTTCGGTCCTCCACGACCTCTTACAGCCGCTTCAACCTG<br /> |rRNA<br /> |-<br /> |CTAACCACAGGTCATCCCCCAGGTTTTCAACCCTGGTGGGTTCGGTCC<br /> |rRNA<br /> |-<br /> |AGAACCAGCTATCACGGAGTTTGATTGGCCTTTCACCCCTAACCACAGGTCATC<br /> |rRNA<br /> |-<br /> |CAAGAAACACACGACGCTGCACCTAAATGCATTTCGGGGAGAACCAGCTATCA<br /> |rRNA<br /> |-<br /> |TTTTAGCCTTGCGGCCGTACTCCCCAGGCGGGGCACTTAATGCGTTAGCTGCGGCAC<br /> |rRNA<br /> |-<br /> |CCGTCAATTCCTTTGAGTTTTAGCCTTGCGGCCGTACTCC<br /> |rRNA<br /> |-<br /> |AATAATCCGACCGTTCACAGCGTCCTCGCTGTTGTGTTACTTCAAAGGAA<br /> |rRNA<br /> |-<br /> |TTCTTCGCGTTGCGTCGAATTAAGCCACATGCTCCGCCGCTTGTGCGGGCCCCCGTCAA<br /> |rRNA<br /> |-<br /> |GTGTATGTCAAGCCTTGGTAAGGTTCTTCGCGTTGCGTCGAATTAAG<br /> |rRNA<br /> |-<br /> |GGGGGCACCATCTCTGATGCTTTCCGGTGTATGTCAAGCC<br /> |rRNA<br /> |-<br /> |TCACGACACGAGCTGACGACAGCCATGCACCACCTGTACACCGACCACAAGGGGGGCACC<br /> |rRNA<br /> |-<br /> |CTTAACCCAACATCTCACGACACGAGCTGACGACAGCCATG<br /> |rRNA<br /> |-<br /> |CAACACGGGACAAGGGTTGCGCTCGTTGCGGGACTTAACC<br /> |rRNA<br /> |-<br /> |GTCTCCCGTGAGTCCCCAACACCCCGAAGGGCTTGCTGGCAAC<br /> |rRNA<br /> |-<br /> |ACATAAGGGGCATGATGACTTGACGTCGTCCCCACCTTCCTCCGAGTTGACCCCG<br /> |rRNA<br /> |-<br /> |ATTGTACCGGCCATTGTAGCACGTGTGCAGCCCAAGACATAAGGGGCATGATGACTTGA<br /> |rRNA<br /> |-<br /> |TTTTGAGATTCGCTCCACCTTGCGGTATCGCAGCTCATTGTACCGGCCATTGTAGCACGT<br /> |rRNA<br /> |-<br /> |CAATCCGAACTGAGACCGGCTTTTTGAGATTCGCTCCACCTTGCGGTAT<br /> |rRNA<br /> |-<br /> |ATTACTAGCGACTCCGACTTCATGGGGTCGAGTTGCAGACCCCAATCCGAACTGAGA<br /> |rRNA<br /> |-<br /> |TATTCACCGCAGCAATGCTGATCTGCGATTACTAGCGACTCCGACTTCATG<br /> |rRNA<br /> |-<br /> |GTGTACAAGGCCCGGGAACGTATTCACCGCAGCAATGCTG<br /> |rRNA<br /> |-<br /> |GTAATCGGGTGACACCACGAGAGCGGAACAGTCGGAGGAATAA<br /> |rRNA<br /> |-<br /> |GTGTTACCGACTTTCGTGACGTGACGGGCGGTGTGTACAAG<br /> |rRNA<br /> |-<br /> |CACCTTCGACAGCTCCCTCCCACAAGGGGTTGGGCCACCGGCTTCGGGTGTTA<br /> |rRNA<br /> |-<br /> |CTACCTTGTTACGACTTCGTCCCAATCGCCAGTCCCACCTTCGACAG<br /> |rRNA<br /> |-<br /> |TCCTTAGAAAGGAGGTGATCCAGCCGCACCTTCCGGTACGGCTACCTTGTTACGA<br /> |rRNA<br /> |-<br /> |TGCAGTCGGCTATGTGCTCCTTAGAAAGGAGGTGATCCAG<br /> |rRNA<br /> |-<br /> |GATCAAACTCTCCGTGAATGTATACCCGTAATCGGGTGAC<br /> |rRNA<br /> |-<br /> |AAGTGGTTCATCGTTCGACTTGCATGTGTTAAGCACGCCGCCAGCGTTCGTCCTGA<br /> |rRNA<br /> |-<br /> |TATCAACATATCTGGCGTTGACTTTTGGCACGCTGTTGAGTTCTCAAGGAA<br /> |rRNA<br /> |-<br /> |TGTTACTCACCCGTTCGCCACTAATCCCCACCGAAGTGGTTCATCGTTCGACTT<br /> |rRNA<br /> |-<br /> |CTTGTCCCAGAGTGAAGGGCAGATTGCCCACGTGTTACTC<br /> |rRNA<br /> |-<br /> |GCAGATGCCTGCGAGGGTCAGTATCCGGTATTAGACCCCGTTTCCAGGGCTTGTCCCA<br /> |rRNA<br /> |-<br /> |ATCCTTCACCGCCGGAGCTTTCGAACCTCGCAGATGCCTGCGAGGGTCAGTATCC<br /> |rRNA<br /> |-<br /> |GATAGGCCGCGGGCTCATCCTTCACCGCCGGAGCTTTCGA<br /> |rRNA<br /> |-<br /> |GTCGCCTTGGTGAGCCATTACCTCACCAACAAGCTGATAG<br /> |rRNA<br /> |-<br /> |TAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGGCCGGTCGCCCTCTCAGGCCGGCTA<br /> |rRNA<br /> |-<br /> |ATTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTC<br /> |rRNA<br /> |-<br /> |CGTCGCTGCATCAGGCTTTCGCCCATTGTGCAATATTCCC<br /> |rRNA<br /> |-<br /> |CTTCTTCCCTGCTGAAAGAGGTTTACAACCCGAAGGCCGTCATC<br /> |rRNA<br /> |-<br /> |TTCAAAGGAACCTCAACCCGAACGGAGATCCGATCAGGTCGGGGTATCAACATAT<br /> |rRNA<br /> |-<br /> |GTAGTTAGCCGGCGCTTCTTCTGCAGGTACCGTCACTTTC<br /> |rRNA<br /> |-<br /> |ATAATTCCGGACAACGCTTGCGCCCTACGTATTACCGCGGCTGCTGGCACGTAGTTA<br /> |rRNA<br /> |-<br /> |CTTTCACAACCGACGTGACAAGCCGCCTACGAGCTCTTTACGCCCAATAATT<br /> |rRNA<br /> |-<br /> |TATCGACTGCAGACCCGGGGTTAAGCCCCGGGCTTTCACAACCGACGTGACAAG<br /> |rRNA<br /> |-<br /> |GATCTCCCCTACCGAACTCTAGCCTGCCCGTATCGACTGCAGAC<br /> |rRNA<br /> |-<br /> |CTGATATCTGCGCATTTCACCGCTACACCAGGAATTCCGATCTC<br /> |rRNA<br /> |-<br /> |CAGAGATCCGCCTTCGCCACCGGTGTTCCTCCTGATATCTG<br /> |rRNA<br /> |-<br /> |CACGCTTTCGCTCCTCAGCGTCAGTATCGGCCCAGAGATC<br /> |rRNA<br /> |-<br /> |CACCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTTC<br /> |rRNA<br /> |-<br /> |ACTTAATGCGTTAGCTGCGGCACGGACAACGTGGAATGTTGCCCACACCTAGTGCCCA<br /> |rRNA<br /> |-<br /> |GTTCGAAATGTAACCGGGCGTTTCCCCTACGCTATGACCACCGAAA<br /> |rRNA<br /> |-<br /> |CTTAGGATGGTTATAGTTACCACCGCCGTTTACTGGCGCTTAAGTTCTCA<br /> |rRNA<br /> |-<br /> |CCAAGCAAGCTTGATGTCCGACGCTCCCACGGCTTGTAGGCACACGGTTTCAGGTACTA<br /> |rRNA<br /> |-<br /> |CAGTCACGAGACACCAAGCAAGCTTGATGTCCGACGCTCC<br /> |rRNA<br /> |-<br /> |CTTGAGCGTGCTACTAAAAGCGCCCTATTCGGACTCGCTT<br /> |rRNA<br /> |-<br /> |CTACCTTGTTACGACTTCGTCCCAATCGCCAGTCCCACCTTCGACAGCTCCCT<br /> |rRNA<br /> |-<br /> |AAGGAGGTGATCCAGCCGCACCTTCCGGTACGGCTACCTTGTTACGACTTCGTC<br /> |rRNA<br /> |-<br /> |CCAGGATCAAACTCTCCGTGAATGTATACCCGTAATCGGG<br /> |rRNA<br /> |-<br /> |CTACCTTGTTACGACTTCGTCCCAATCGCCAGTCCCACCTTCG<br /> |rRNA<br /> |-<br /> |CTCTCACTTACCGGCATTCGGAGTTTGGCTAAGGTCAGTAAC<br /> |rRNA<br /> |-<br /> |CATTCGGACACCCCCGGATCAAAGCCTGGTTGACGACTCC<br /> |rRNA<br /> |-<br /> |GCTTATCCCCCACAGTCTCACTGCCGCGCTCTCACTTACC<br /> |rRNA<br /> |-<br /> |CTTAGTCGATGCTCTGGGCTGTTTCCCTCTCGACCATGGAGCTTATC<br /> |rRNA<br /> |-<br /> |CACATCCTTTCCCACTTAGCGTACGCTTAGGGGCCTTAGTCGATGCTCTG<br /> |rRNA<br /> |-<br /> |CTTCTAAGCCAACCTCCTGGTTGTCTGTGCGACTCCACATCCTTTC<br /> |rRNA<br /> |-<br /> |AATCACTAGACCAGTGAGCTATTACGCACTCTTTCAAGGGTGGCTGCTTCTAAGC<br /> |rRNA<br /> |-<br /> |CTACATTGTCGGCGCGGAATCACTAGACCAGTGAGCTATTA<br /> |rRNA<br /> |-<br /> |CGTATTGCTGCAATGACACGACTTCGGCGGTACGCTTGAG<br /> |rRNA<br /> |-<br /> |GCTCCCCTACCCATCACAGCCGGCGTTGGCCGTATTGCTG<br /> |rRNA<br /> |-<br /> |TTCTCACTCGTGAACCGTCCACAACTCGCTTCCGCGGCTGCTTCACCCGGCACACGACG<br /> |rRNA<br /> |-<br /> |GTTTGTATCGCTACTCATGCCTGCATTCTCACTCGTGAACCGTCCACAAC<br /> |rRNA<br /> |-<br /> |CTTAGTCAATCGGCGCAAACGTTTCTCACGTTTGTATCGCTACTCATG<br /> |rRNA<br /> |-<br /> |GACTTACCCTGGGCAGATCAGCTTGACCCAGGAACCCTTAGTCAATC<br /> |rRNA<br /> |-<br /> |TCCACACTGCCTATGTGTTCAGCAGCGGGTGACAGCCCATGACGACTGCCGGGTTT<br /> |rRNA<br /> |-<br /> |TTATCCATCGACTACGCCTGTCGGCCTCGCCTTAGGTCCCGACTTACCCTGGGCA<br /> |rRNA<br /> |-<br /> |GTACCGGAATATCAACCGGTTATCCATCGACTACGCCTGTC<br /> |rRNA<br /> |-<br /> |GGCCTTAGCATCACGATGCTCGATGTTTGACGCTTCACAG<br /> |rRNA<br /> |-<br /> |CTACTAACCGCTTGGTTCGGCGGCTCCACCACTCCCCTCAACTCCGAAGAGATCA<br /> |rRNA<br /> |-<br /> |GATGGACTACCTTCCTGCGTCACCCCATCACTCACCTACTAAC<br /> |rRNA<br /> |-<br /> |CCGGCCTACACCCTTACCCCGGGACAACCACCGCCCGGGATGGACTACC<br /> |rRNA<br /> |-<br /> |CAAGGCATCCACCGTGCGCCCTTAAAAACTTGGCCACAGA<br /> |rRNA<br /> |-<br /> |CAAGGTGTGCGGATTTACCTACACACCGGCCTACACCCTTAC<br /> |rRNA<br /> |-<br /> |CCACAATCGGCTCGGCATCAGGTCTCAGCCACAAGGTGTG<br /> |rRNA<br /> |-<br /> |CTAGAGGCTTTTCTCGACAGCATAGGATCATCCACTTCACCACAATC<br /> |rRNA<br /> |-<br /> |TTTAGGGTACGGGCCGCCATGAAACTCGCTAGAGGCTTTTCTC<br /> |rRNA<br /> |-<br /> |CTCGGTATTCTCTACCTGACCACCTGAGTCGGTTTAGGGTAC<br /> |rRNA<br /> |-<br /> |GGCATTTTGCCGAGTTCCTTAACCATAGTTCACCCGAACG<br /> |rRNA<br /> |-<br /> |CAGCTCAGAGTGCAAAACTCGTCACCAGGTGTGGCCCCCCTTCTCCCGAAGTTA<br /> |rRNA<br /> |-<br /> |TTGTTTTCTCTTCCTGCGGGTACTGAGATGTTTCACTTCCCCGCGTTCCCTCCACA<br /> |rRNA<br /> |-<br /> |TGTGTTTTTAGTAAACAGTCGCTTCTCGCTGGTCTCTGCGGCCACCCCCAGCTCAGA<br /> |rRNA<br /> |-<br /> |TATACATCGCCTTACGGCTTCGCACGGACCTGTGTTTTTAGTAAACAGTCGCTTCT<br /> |rRNA<br /> |-<br /> |GCAGGCGTCAGTCCGTATACATCGCCTTACGGCTTCGCAC<br /> |rRNA<br /> |-<br /> |GAAATGTGACTAACCGGTCCCCTTAACGTTCCAGCACCGGGCAGGC<br /> |rRNA<br /> |-<br /> |GTTTACTGGCGCTTAAGTTCTCAGCTTCGCCACACCGAAATGTGACTAAC<br /> |rRNA<br /> |-<br /> |GACAAGGAATTTCGCTACCTTAGGATGGTTATAGTTACCACCGCCGTTTA<br /> |rRNA<br /> |-<br /> |AAGTCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTA<br /> |rRNA<br /> |-<br /> |CTATGGTTGAGACAGTCGAGAAGTCGTTACGCCATTCGTG<br /> |rRNA<br /> |-<br /> |CTGCGCGAAACGAGCATCTTTACTCGTAGTGCAATTTCAC<br /> |rRNA<br /> |-<br /> |CAATATCAAACTGTAGTAAAGGTCCCGGGGTCTTTCCGTCCTGCTGCGCGAAA<br /> |rRNA<br /> |-<br /> |CAGCTATCCTACACAAGCCGAACCGAACACCAATATCAAACTGTAGTAAA<br /> |rRNA<br /> |-<br /> |CACACTGGCGTGCGAGCTTCAAAGTCTCCCAGCTATCCTACACAAG<br /> |rRNA<br /> |-<br /> |TTAGACATCCAGCACGACCAGACTGGTATTTCAACGACGACTCCACCCACACTGGCGTG<br /> |rRNA<br /> |-<br /> |GTTTCGCTCGCCACTACTCCCGGAATCACGGTTGTTTTCTCTTC<br /> |rRNA<br /> |-<br /> |GATCACGGACCCAGGTTAGACATCCAGCACGACCAGACTG<br /> |rRNA<br /> |-<br /> |CAGTTAAACTACCCATCAGACACTGTCCCTGATCCGGATCAC<br /> |rRNA<br /> |-<br /> |GAACCTTTGGGCGCCTCCGTTACTCTTTAGGAGGCAACCG<br /> |rRNA<br /> |-<br /> |CTTGTGCACTTACACTCAACACCTGATTGCCAACCAGGCTGAGGGAACCTTT<br /> |rRNA<br /> |-<br /> |GACCCGTCGGTCTCACAGTCAAGCTCCCTTGTGCACTTAC<br /> |rRNA<br /> |-<br /> |CTTCCACAAGCCACCGCCGGATCACTAGTCCCGACTTTCGTCCCTG<br /> |rRNA<br /> |-<br /> |GTACCTTTTATCCGTTGAGCGACGGCGCTTCCACAAGCCAC<br /> |rRNA<br /> |-<br /> |CAAACCATCCCGTCGATATGGACTCTTGGGGAAGATCAGCCTGTTATC<br /> |rRNA<br /> |-<br /> |CCAGGATGCGACGAGCCGACATCGAGGTGCCAAACCATCC<br /> |rRNA<br /> |-<br /> |GTCTCACACGTATACGGTTTGGCCTCATCCGGTTTCGCTC<br /> |rRNA<br /> |-<br /> |CTTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTCCAG<br /> |rRNA<br /> |-<br /> |GAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCGCTTTAATG<br /> |rRNA<br /> |-<br /> |CTTCTCAAGACTCCTACGCGCACAGCGGATAGGGACCGAAC<br /> |rRNA<br /> |-<br /> |CACACCAGAGGTTCGTCCGTCCCGGTCCTCTCGTACTAGG<br /> |rRNA<br /> |-<br /> |TTAGATGCTTTCAGCGGTTATCCCTCCCGAACGTAGCCAACCAGCCATGCCCTTGGCA<br /> |rRNA<br /> |-<br /> |CTCATCTCGAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATC<br /> |rRNA<br /> |-<br /> |CAGTCGTCTACTGGGAGCCTTAACCCCTCAAGGGGGTGGGAGTCCTCATCTCGAA<br /> |rRNA<br /> |-<br /> |CTTCCAGATCCGGCCTATCAACCCAGTCGTCTACTGGGAG<br /> |rRNA<br /> |-<br /> |CAGACCGTAAAAGAGAGATCCCACAACCCCGCACACGCAACCCCTGCCGGGTCTCA<br /> |rRNA<br /> |-<br /> |TACATCAACGGTTTCTGACTCGTCTCACGGCCGGCAGACCGTAAAAGAGAGAT<br /> |rRNA<br /> |-<br /> |CTTTCGCATGTCCTTCGCCTACATCAACGGTTTCTGACTC<br /> |rRNA<br /> |-<br /> |ACTACGGGGGTCTTACCCTCTACGCCGGACCTTTCGCATG<br /> |rRNA<br /> |-<br /> |CTACTTGGGTGTCTCTCAAACGAGCCGTTGACGTTTCGACTAC<br /> |rRNA<br /> |-<br /> |GCCAGATTCACACGGGATTTCTCGGGCCCCGTGCTACTTG<br /> |rRNA<br /> |-<br /> |TATCCGCTATCGGTCACCAGGGAATATTTAGGCTTAGCGGGTGGTCCCGCCAGATT<br /> |rRNA<br /> |-<br /> |GTACTTTTCACCATTCCCTCACGGTACTATCCGCTATCGGTCAC<br /> |rRNA<br /> |-<br /> |CTTGTAGGCACACGGTTTCAGGTACTATTTCACTCCGCTCCC<br /> |rRNA<br /> |-<br /> |GGGCCTATCGTGGCCTCCCACGTCCTTCATCGGTTCCTGG<br /> |rRNA<br /> |-<br /> |CACGCAGTCACGAGGATGGAGCAAGCTCCATCCCGACGCTCCCACGGCTTGTAGGCA<br /> |rRNA<br /> |-<br /> |CATTCTTCAAAAGGCACGCAGTCACGAGGATGGAGCAAGC<br /> |rRNA<br /> |-<br /> |TACGGCTTCCCCACTCGGGTTAACCTCGCAACACACCGCAAACTCGCAGGCTCAT<br /> |rRNA<br /> |-<br /> |TCGGACTCGCTTTCGCTACGGCTTCCCCACTCGGGTTAAC<br /> |rRNA<br /> |-<br /> |ATCACTCCGCTTCGGGTCTTGAGCGTGCTACTAAAAACGC<br /> |rRNA<br /> |-<br /> |CTCTTACAGCCGCTTCAACCTGCCCATGGCTAGATCACTC<br /> |rRNA<br /> |-<br /> |GTTTTCAACCCTGGTGGGTTCGGTCCTCCACGACCTCTTAC<br /> |rRNA<br /> |-<br /> |GTTTGATTGGCCTTTCACCCCTAACCACAGGTCATCCCCCAGGTTTTCAACC<br /> |rRNA<br /> |-<br /> |GGAGAACCAGCTATCACGGAGTTTGATTGGCCTTTCACCC<br /> |rRNA<br /> |-<br /> |TATCCAGTGCTCTACCTCCGGCAAGAAACACACGACGCTGCACCTAAATGCATTT<br /> |rRNA<br /> |-<br /> |GAGTTTGGCTAAGGTCAGTAACCCGGTAGGGCCCATCGCCTATCCAGTGCTCTA<br /> |rRNA<br /> |-<br /> |CTGTAAGGCTTAGCTTCCGGGTTCGAAATGTAACCGGGCGTTTCCCCTAC<br /> |rRNA<br /> |-<br /> |GTTCGGCGGCGTCCTACTCTCCCACAGGGTCCCCCCTGCAGTACCATCGGCGCTGTA<br /> |rRNA<br /> |-<br /> |GTATTAGACCCCGTTTCCAGGGCTTGTCCCAGAGTGAAGGGCAGATT<br /> |rRNA<br /> |-<br /> |GAGCTTTCGAACCTCGCAGATGCCTGCGAGGGTCAGTATCCGGTATTAGACCCCGTT<br /> |rRNA<br /> |-<br /> |CATCCTTCACCGCCGGAGCTTTCGAACCTCGCAGATGCCT<br /> |rRNA<br /> |-<br /> |CACCGAAGTGGTTCATCGTTCGACTTGCATGTGTTAAGCAC<br /> |rRNA<br /> |-<br /> |TTCGACTTGCATGTGTTAAGCACGCCGCCAGCGTTCGTCCTGAGCCAGGATCAAACTCT<br /> |rRNA<br /> |-<br /> |CAGATTGCCCACGTGTTACTCACCCGTTCGCCACTAATCC<br /> |rRNA<br /> |-<br /> |GCGGATTTACCTACCTGACGTCCTACACCCTTACCCCGGGACAACCACCGC<br /> |rRNA<br /> |-<br /> |CATCAGGTCTCAGACTCATGTCAGGCGGATTTACCTACCTGAC<br /> |rRNA<br /> |-<br /> |CTATTAGTACCGGTCACCTCCACACCTCACGGTGCTTCCAGATCCGGCCTATCAACC<br /> |rRNA<br /> |-<br /> |GGACAAGCCCTCGGCCTATTAGTACCGGTCACCTCCACAC<br /> |rRNA<br /> |-<br /> |CCGGGTTAACCTCGCAACACACCGCAAACTCGCAGGCTCATTCTTCAAAAGGCACGC<br /> |rRNA<br /> |-<br /> |GGTCTTGAGCGTGCTACTAAAACGCCCTATTCGGACTCGC<br /> |rRNA<br /> |-<br /> |TTCGGCGGCGTCCTACTCTCCCACAGGGTCCCCCCTGCAGTACCATCGGCGCTGTAA<br /> |rRNA<br /> |-<br /> |CCGAAGCCTCCGGCGGTCTGCTTTCTGTGGCACTGTCCCG<br /> |SCOs01<br /> |-<br /> |CTGGGCACTGGTGGTCTCTTACACCACCGTTTCACCCTTAC<br /> |SCOs01<br /> |-<br /> |TTCCTTCTTGACCTTGCTCCGGGTGGGGTTTACCTAGCTGCCCAGGTCGCCCTG<br /> |SCOs01<br /> |-<br /> |CTCGAACGTCCGCGCAGAAGCACCGGGGTGCTTCCTTCTTGACCTTGCTC<br /> |SCOs01<br /> |-<br /> |CTCCAGCGGTCTACCCGCGGACTCGGGCGGGCAGCCCTCGAACGTCCGCGCAGAAG<br /> |SCOs01<br /> |-<br /> |CATCCATCTAGGACCGGCGTTGCCACCGGCCTCCAGCGGTCTAC<br /> |SCOs01<br /> |-<br /> |CTGTGGAGCCCGGACGTTCCTCGGGAAGTCCCGTGAGGGACTC<br /> |SCOs01<br /> |-<br /> |GTTCTGTTCCGACGGGGCCTCGCGGTCCCGTCGGCGACGGCCATCCAT<br /> |SCOs01<br /> |-<br /> |CAGACGAGTCGGGCTGTACGCCGGGTTCTGTTCCGACGGG<br /> |SCOs01<br /> |-<br /> |GTCTGCTTTCTGTGGCACTGTCCCGCGGGTCACCCCGGGTGGCCGTTAGCCATCAC<br /> |SCOs01<br /> |-<br /> |CTTAGTAGGAGGCTCGCTTCGCTACCTGAGATCAGGCAGCGAGGGCGAAGG<br /> |SCOs02<br /> |-<br /> |GAACAGCCCCGGGCTGACACTCCCTTAGTAGGAGGCTCGCTTC<br /> |SCOs02<br /> |-<br /> |GATCAAGGTTTAGTCCCCTAGCTGATGCCAGGATCCGGGTC<br /> |SCOs02<br /> |-<br /> |CAGTCACTGTTTGGTTTCCCTCTTCACCCCGTGACCGGGATCAA<br /> |SCOs02<br /> |-<br /> |GAGATCACGCGGACAAGTCTCCGACGGGCCCAGTCACTGT<br /> |SCOs02<br /> |-<br /> |CTTCCCCTGCTTCGACAGCCGCTGTCGAAACCGATCATCC<br /> |SCOs02<br /> |-<br /> |AATCAGGGCTTCTCCGTGTGCAGTCCGCTTCGATTTTCTCGGCCCCGGAGATCACG<br /> |SCOs02<br /> |-<br /> |GAATCGAACCCGCGTCCAACGGTGCGGAATCAGGGCTTCTC<br /> |SCOs02<br /> |-<br /> |TTTTTCGGCCTGTGGTTTACGAGATCATGGCCGCTTCCTC<br /> |SCOs02<br /> |-<br /> |CTGCTGGGAGGAATCGCGCTTGGTGTTGGCGATTATTTTT<br /> |SCOs02<br /> |-<br /> |CACGCACCCGCCAGAGCCGACCTACCCTTGCTGCCTTCCG<br /> |SCOs03<br /> |}<br /> <br /> ==Sign off==<br /> <br /> Page created by R. T. Evans, University of Brighton.<br /> stonybonytony@gmail.com</div> RTEvans https://actinobase.org/index.php?title=RRNA_depletion_with_Streptomyces_coelicolor_A3(2)_specific_probes&diff=2198 RRNA depletion with Streptomyces coelicolor A3(2) specific probes 2023-11-01T16:53:08Z <p>RTEvans: </p> <hr /> <div>==Organisms==<br /> <br /> This protocol has been tested with &lt;em&gt;[[Streptomyces coelicolor]]&lt;/em&gt; A3(2) MT1110<br /> <br /> ==Description==<br /> <br /> rRNA depletion using commercial kits targeting generic bacterial sequences is not 100% efficient in the High GC organism &lt;em&gt;S. coelicolor&lt;/em&gt;. <br /> <br /> Depletion using the NEBNext RNA Depletion Core Reagent Set with RNA Sample Purification Beads (https://www.neb.com/en-gb/products/e7870-nebnext-rna-depletion-core-reagent-set-with-rna-sample-purification-beads#Product%20Information) and custom DNA probes designed for &lt;em&gt;S. coelicolor&lt;/em&gt; is more effective than the QIAseq FastSelect 5S/16S/23S kit (Qiagen #335927); NEBNext rRNA Depletion Kit (Bacteria) (NEB #E7850); and Illumina Ribo-zero rRNA Removal Kit (Bacteria) (Illumina #MRZMB12424, discontinued). The Illumina kit tested was subsequently replaced by the Illumina Ribo-Zero Plus rRNA Depletion Kit (Illumina #20040526). <br /> <br /> In addition, three abundant non-mRNA RNAs can also be depleted using the custom probes (Table 1).<br /> <br /> '''Table 1.''' Abundant non-mRNA RNAs in &lt;em&gt;S. coelicolor&lt;/em&gt;.<br /> <br /> [[File: rRNA_depletion_table_1.png]]<br /> <br /> ==Materials==<br /> <br /> Single stranded DNA probes at 2µM concentration in IDTE pH 7.5 (10mM Tris-HCl / 0.1mM EDTA). Integrated DNA Technologies can produce these from their 25nmole DNA oligo pool product. Probes are listed in Table 2 in the Notes section below.<br /> <br /> NEBNext RNA Depletion Core Reagent Set with RNA Sample Purification Beads (https://www.neb.com/en-gb/products/e7870-nebnext-rna-depletion-core-reagent-set-with-rna-sample-purification-beads#Product%20Information).<br /> <br /> ==Protocols==<br /> <br /> As per manufacturer’s instructions.<br /> <br /> ==Notes==<br /> <br /> The following histograms illustrate effectiveness of rRNA depletion using various methods. Histograms show percentage of bases aligned to NCBI reference sequence NC_003888.3 using Hisat2 program classified as follows - Coding = RNA aligned to coding sequences; Transcribed not translated = RNA aligned to rRNA, tRNA and sRNA; Intergenic = RNA aligned to 5' or 3' UTRs or between annotated sequences.<br /> <br /> '''QIAseq FastSelect 5S/16S/23S kit'''<br /> <br /> [[File: PanelA.png]] <br /> <br /> '''NEBNext rRNA Depletion Kit (Bacteria)'''<br /> <br /> [[File: PanelB.png]]<br /> <br /> '''Illumina Ribo-zero rRNA Removal Kit (Bacteria)'''<br /> <br /> [[File: PanelC.png]] <br /> <br /> '''rRNA depletion with &lt;em&gt;S. coelicolor&lt;/em&gt; specific probes<br /> <br /> [[File: PanelD.png]]<br /> <br /> <br /> '''Table 2''' List of probes<br /> <br /> {| class=&quot;wikitable&quot;<br /> !Sequence<br /> !Target<br /> |-<br /> |CAAGTCCTTGCGCGACGCTCCCACGGCTTGTAGGCACACGGTTTCAGGTACTATTT<br /> |rRNA<br /> |-<br /> |CAGTCACGACGCAAGGACAAGTCCTTGCGCGACGCTCCCAC<br /> |rRNA<br /> |-<br /> |ACCTCGCAACACACCGCAAACTCGCAGGCTCATTCTTCAAAAGGCACGCAGTCACGACG<br /> |rRNA<br /> |-<br /> |CTACGGCTTCCCCACCCGGGTTAACCTCGCAACACACCGC<br /> |rRNA<br /> |-<br /> |TTAACCCAACATCTCACGACACGAGCTGACGACAGCCATGCACCACCTGTACACCGA<br /> |rRNA<br /> |-<br /> |CGCTCGTTGCGGGACTTAACCCAACATCTCACGACACGAG<br /> |rRNA<br /> |-<br /> |CAACACCCCGAAGGGCTTGCTGGCAACACGGGACAAGGGTTG<br /> |rRNA<br /> |-<br /> |CACCTTCCTCCGAGTTGACCCCGGCGGTCTCCCGTGAGTC<br /> |rRNA<br /> |-<br /> |GACATAAGGGGCATGATGACTTGACGTCGTCCCCACCTTC<br /> |rRNA<br /> |-<br /> |GTATCGCAGCTCATTGTACCGGCCATTGTAGCACGTGTGCAGCCCAAGACATAA<br /> |rRNA<br /> |-<br /> |TTTTGAGATTCGCTCCACCTTGCGGTATCGCAGCTCATTGTAC<br /> |rRNA<br /> |-<br /> |CAGACCCCAATCCGAACTGAGACCGGCTTTTTGAGATTCG<br /> |rRNA<br /> |-<br /> |AATGCTGATCTGCGATTACTAGCGACTCCGACTTCATGGGGTCGAGTTGCAGAC<br /> |rRNA<br /> |-<br /> |GAACGTATTCACCGCAGCAATGCTGATCTGCGATTACTAG<br /> |rRNA<br /> |-<br /> |TACCGACTTTCGTGACGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTC<br /> |rRNA<br /> |-<br /> |GCCACCGGCTTCGGGTGTTACCGACTTTCGTGACGTGACG<br /> |rRNA<br /> |-<br /> |TTCGTCCCAATCGCCAGTCCCACCTTCGACAGCTCCCTCCCACAAGGGGTTGGG<br /> |rRNA<br /> |-<br /> |CGCACCTTCCGGTACGGCTACCTTGTTACGACTTCGTCCCAATCGCCAGTCCCA<br /> |rRNA<br /> |-<br /> |TTCGACCCTCGCAGATGCCTGCGAGGGTCAGTATCCGGTATTAGACCCCGTTTCCAG<br /> |rRNA<br /> |-<br /> |GAAAGGAGGTGATCCAGCCGCACCTTCCGGTACGGCTACC<br /> |rRNA<br /> |-<br /> |TTCACCGCCGGAGCTTTCGACCCTCGCAGATGCCTGCGAG<br /> |rRNA<br /> |-<br /> |CATTACCTCACCAACAAGCTGATAGGCCGCGGGCTCATCCTTC<br /> |rRNA<br /> |-<br /> |CTCTCAGGCCGGCTACCCGTCGTCGCCTTGGTGAGCCATTAC<br /> |rRNA<br /> |-<br /> |GTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGGCCGGTCG<br /> |rRNA<br /> |-<br /> |TGCATCAGGCTTTCGCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCT<br /> |rRNA<br /> |-<br /> |TGCTGAAAGAGGTTTACAACCCGAAGGCCGTCATCCCTCACGCGGCGTCGCTGCATCA<br /> |rRNA<br /> |-<br /> |TTCTTCTGCAGGTACCGTCACTTTCGCTTCTTCCCTGCTGAAAGAGGTTTACAA<br /> |rRNA<br /> |-<br /> |CTACGTATTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTCTG<br /> |rRNA<br /> |-<br /> |CTCTTTACGCCCAATAATTCCGGACAACGCTTGCGCCCTACGTATTAC<br /> |rRNA<br /> |-<br /> |GGGCTTTCACAACCGACGTGACAAGCCGCCTACGAGCTCT<br /> |rRNA<br /> |-<br /> |CTACCGAACTCTAGCCTGCCCGTATCGACTGCAGACCCGGGGTTAAGCCCCG<br /> |rRNA<br /> |-<br /> |ATATCTGCGCATTTCACCGCTACACCAGGAATTCCGATCTCCCCTACCGAACTCTAG<br /> |rRNA<br /> |-<br /> |CGGTGTTCCTCCTGATATCTGCGCATTTCACCGCTACACC<br /> |rRNA<br /> |-<br /> |ACGCTTTCGCTCCTCAGCGTCAGTATCGGCCCAGAGATCCGCCTTCGCCACCGGTGT<br /> |rRNA<br /> |-<br /> |GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCG<br /> |rRNA<br /> |-<br /> |ACAACGTGGAATGTTGCCCACACCTAGTGCCCACCGTTTACGGCGTGGACTACCA<br /> |rRNA<br /> |-<br /> |CTTAATGCGTTAGCTGCGGCACGGACAACGTGGAATGTTG<br /> |rRNA<br /> |-<br /> |TCAATTCCTTTGAGTTTTAGCCTTGCGGCCGTACTCCCCAGGCGGGGCACTTAA<br /> |rRNA<br /> |-<br /> |ATTAAGCCACATGCTCCGCCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTTA<br /> |rRNA<br /> |-<br /> |TCAAGCCTTGGTAAGGTTCTTCGCGTTGCGTCGAATTAAGCCACATGCTCCGCCGCTT<br /> |rRNA<br /> |-<br /> |CATCTCTGATGCTTTCCGGTGTATGTCAAGCCTTGGTAAGGTTCTTC<br /> |rRNA<br /> |-<br /> |ACAGCCATGCACCACCTGTACACCGACCACAAGGGGGGCACCATCTCTGATGCTTTCC<br /> |rRNA<br /> |-<br /> |TGTAACCGGGCGTTTCCCCTACGCTATGACCACCGAAACC<br /> |rRNA<br /> |-<br /> |CAGTACCATCGGCGCTGTAAGGCTTAGCTTCCGGGTTCGAAATGTAAC<br /> |rRNA<br /> |-<br /> |GTTCGGCGGCGTCCTACTCTCCCACAGGGTCCCCCCTGCA<br /> |rRNA<br /> |-<br /> |CACTCCCCTCAACTCCGAAGAGATCAGGGCGGCTTCACGG<br /> |rRNA<br /> |-<br /> |CCATCACTCACCTACTAACCGCTTGGTTCGGCGGCTCCAC<br /> |rRNA<br /> |-<br /> |GTCCTTCATCGGTTCCTGGTGCCAAGGCATCCACCGTGCGCCCTTAAAAACTT<br /> |rRNA<br /> |-<br /> |CTTGTAGGCACACGGTTTCAGGTACTATTTCACTCCGCTCCCG<br /> |rRNA<br /> |-<br /> |CACGCAGTCACGAGACACCAAGCAAGCTTGATGTCCGACGCTCCCACGGCTTGTAGGCAC<br /> |rRNA<br /> |-<br /> |TCTTCAAAAGGCACGCAGTCACGAGACACCAAGCAAGCTT<br /> |rRNA<br /> |-<br /> |CACCCGGGTTAACCTCGCAACACACTGCAAACTCGCAGGC<br /> |rRNA<br /> |-<br /> |GCGTGCTACTAAAACGCCCTATTCGGACTCGCTTTCGCTACGGCTTCC<br /> |rRNA<br /> |-<br /> |GATCACTCCGCTTCGGGTCTTGAGCGTGCTACTAAAACGC<br /> |rRNA<br /> |-<br /> |TCGCAGATGCCTGCAAGGGTCAGTATCCGGTATTAGACCCCGTTT<br /> |rRNA<br /> |-<br /> |TCATCCTTCACCGCCGCAGCTTTCGAACCTCGCAGATGCC<br /> |rRNA<br /> |-<br /> |ATGTAACCGGGCGTTTCCCCTACGCTATGACCACCGAAAC<br /> |rRNA<br /> |-<br /> |CGTCCTACTCTCCCACAGGGTCCCCCCTGCAGTACCATCG<br /> |rRNA<br /> |-<br /> |CATCGCCTATCCAGTGCTCTACCTCCGGCAAGAAACACAC<br /> |rRNA<br /> |-<br /> |CATTCGGAGTTTGGCTAAGGTCAGTAACCCGGTAGGGCCCATCG<br /> |rRNA<br /> |-<br /> |CAGTCTCACTGCCGCGCTCTCACTTACCGGCATTCGGAGTTTG<br /> |rRNA<br /> |-<br /> |ATGCTCTGGGCTGTTTCCCTCTCGACCATGGAGCTTATCCCCCACAGTCTCACTG<br /> |rRNA<br /> |-<br /> |CTTAGGGGCCTTAGTCGATGCTCTGGGCTGTTTCCCTCTCG<br /> |rRNA<br /> |-<br /> |GTTGTCTGTGCGACTCCACATCCTTTCCCACTTAGCGTACGCTTAG<br /> |rRNA<br /> |-<br /> |TATTACGCACTCTTTCAAGGGTGGCTGCTTCTAAGCCAACCTCCTGGTTGTCTGT<br /> |rRNA<br /> |-<br /> |GATCAAAGCCTGGTTGACGACTCCCCGGGGCCTATCGTGG<br /> |rRNA<br /> |-<br /> |CGGAATCACTAGACCAGTGAGCTATTACGCACTCTTTCAAGG<br /> |rRNA<br /> |-<br /> |TATTGCTGCAATGACACGACTTCGGCGGTACGCTTGAGCCCCGCTACATTGTCG<br /> |rRNA<br /> |-<br /> |CATCACAGCCGGCGTTGGCCGTATTGCTGCAATGACACGACTTC<br /> |rRNA<br /> |-<br /> |TCCACAACTCGCTTCCGCGGCTGCTTCACCCGGCACACGACGCTCCCCTACCCATCACA<br /> |rRNA<br /> |-<br /> |TACTCATGCCTGCATTCTCACTCGTGAACCGTCCACAACTCGCTTCCGCGGCTGCTT<br /> |rRNA<br /> |-<br /> |CAAACGTTTCTCACGTTTGTATCGCTACTCATGCCTGCATTCTCACTC<br /> |rRNA<br /> |-<br /> |CAGGAACCCTTAGTCAATCGGCGCAAACGTTTCTCACGTTT<br /> |rRNA<br /> |-<br /> |CTTAGGTCCCGACTTACCCTGGGCAGATCAGCTTGACCCAG<br /> |rRNA<br /> |-<br /> |GAATATCAACCGGTTATCCATCGACTACGCCTGTCGGCCTCGCCTTAGGTC<br /> |rRNA<br /> |-<br /> |TTAGCATCACGATGCTCGATGTTTGACGCTTCACAGCGGGTACCGGAATATCAA<br /> |rRNA<br /> |-<br /> |GAAGAGATCAGGGCGGCTTCACGGCCTTAGCATCACGATG<br /> |rRNA<br /> |-<br /> |CATCACTCACCTACTAACCGCTTGGTTCGGCGGCTCCACC<br /> |rRNA<br /> |-<br /> |TGTGTTCAGCAGCGGGTGACAGCCCATGACGACTGCCGGGTTTCCCCATTCGGACACCC<br /> |rRNA<br /> |-<br /> |GACAACCACCGCCCGGGATGGACTACCTTCCTGCGTCACCCCAT<br /> |rRNA<br /> |-<br /> |CAGATGCTCGCGTCCACTGTGCAGTTCTCAAACAACGACC<br /> |rRNA<br /> |-<br /> |TGCGGATTTACCTACACACCGGCCTACACCCTTACCCCGGGACAACCACCG<br /> |rRNA<br /> |-<br /> |CATCAGGTCTCAGCCACAAGGTGTGCGGATTTACCTACAC<br /> |rRNA<br /> |-<br /> |TTTTCTCGACAGCATAGGATCATCCACTTCACCACAATCGGCTCGGCATCAGGTCT<br /> |rRNA<br /> |-<br /> |CCATGAAACTCGCTAGAGGCTTTTCTCGACAGCATAGGATCATC<br /> |rRNA<br /> |-<br /> |TATTCTCTACCTGACCACCTGAGTCGGTTTAGGGTACGGGCCGCCATGAAACT<br /> |rRNA<br /> |-<br /> |GAGTTCCTTAACCATAGTTCACCCGAACGCCTCGGTATTCTCTACCTGAC<br /> |rRNA<br /> |-<br /> |CTTCTCCCGAAGTTACGGGGGCATTTTGCCGAGTTCCTTAACCA<br /> |rRNA<br /> |-<br /> |CACCCCCAGCTCAGAGTGCAAAACTCGTCACCAGGTGTGG<br /> |rRNA<br /> |-<br /> |GTGTTTTTAGTAAACAGTCGCTTCTCGCTGGTCTCTGCGG<br /> |rRNA<br /> |-<br /> |GTTCCCTCCACACTGCCTATGTGTTCAGCAGCGGGTGACAG<br /> |rRNA<br /> |-<br /> |GTATACATCGCCTTACGGCTTCGCACGGACCTGTGTTTTTA<br /> |rRNA<br /> |-<br /> |CTTAACGTTCCAGCACCGGGCAGGCGTCAGTCCGTATACATC<br /> |rRNA<br /> |-<br /> |TAAGTTCTCAGCTTCGCCACACCGAAATGTGACTAACCGGTCCCCTTAACGTT<br /> |rRNA<br /> |-<br /> |TTAGGATGGTTATAGTTACCACCGCCGTTTACTGGCGCTTAAGTTCTCAG<br /> |rRNA<br /> |-<br /> |GTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGATGGTTATAGT<br /> |rRNA<br /> |-<br /> |TATGGTTGAGACAGTCGAGAAGTCGTTACGCCATTCGTGCAGGTCGGAACTTACC<br /> |rRNA<br /> |-<br /> |GAAACGAGCATCTTTACTCGTAGTGCAATTTCACCGGGCCTATGGTTGAG<br /> |rRNA<br /> |-<br /> |GGTCTTTCCGTCCTGCTGCGCGAAACGAGCATCTTTACTC<br /> |rRNA<br /> |-<br /> |GAACCGAACACCAATATCAAACTGTAGTAAAGGTCCCGGGGTCTTTC<br /> |rRNA<br /> |-<br /> |CTTCAAAGTCTCCCAGCTATCCTACACAAGCCGAACCGAACACCAATA<br /> |rRNA<br /> |-<br /> |AGACTGGTATTTCAACGACGACTCCACCCACACTGGCGTGCGAGCTTCAAAGTCT<br /> |rRNA<br /> |-<br /> |GTTAGACATCCAGCACGACCAGACTGGTATTTCAACGACGACTC<br /> |rRNA<br /> |-<br /> |TTAAACTACCCATCAGACACTGTCCCTGATCCGGATCACGGACCCAGGTTAGACAT<br /> |rRNA<br /> |-<br /> |GAATCACGGTTGTTTTCTCTTCCTGCGGGTACTGAGATGTTTCACTTC<br /> |rRNA<br /> |-<br /> |GTTACTCTTTAGGAGGCAACCGCCCCAGTTAAACTACCCATCAGACACTG<br /> |rRNA<br /> |-<br /> |ACTCAACACCTGATTGCCAACCAGGCTGAGGGAACCTTTGGGCGCCTCCGTTACT<br /> |rRNA<br /> |-<br /> |GTCTCACAGTCAAGCTCCCTTGTGCACTTACACTCAACACCTGATTG<br /> |rRNA<br /> |-<br /> |GACTTTCGTCCCTGCTCGACCCGTCGGTCTCACAGTCAAG<br /> |rRNA<br /> |-<br /> |TACCTTTTATCCGTTGAGCGACGGCGCTTCCACAAGCCACCGCCGGATCACTAGTC<br /> |rRNA<br /> |-<br /> |GTCGATATGGACTCTTGGGGAAGATCAGCCTGTTATCCCCGGGGTACCTTTTAT<br /> |rRNA<br /> |-<br /> |GACATCGAGGTGCCAAACCATCCCGTCGATATGGACTCTTG<br /> |rRNA<br /> |-<br /> |TTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTCCAGCCCCAGGATGCGACGA<br /> |rRNA<br /> |-<br /> |TCACGACGTTCTAAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACC<br /> |rRNA<br /> |-<br /> |GATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTC<br /> |rRNA<br /> |-<br /> |GTCCTCTCGTACTAGGGACAGCCCTTCTCAAGACTCCTAC<br /> |rRNA<br /> |-<br /> |TCTCACACGTATACGGTTTGGCCTCATCCGGTTTCGCTCGCCACTACTCCCGGAAT<br /> |rRNA<br /> |-<br /> |CATGCCCTTGGCAGGACAACTGGCACACCAGAGGTTCGTC<br /> |rRNA<br /> |-<br /> |CTTAGATGCTTTCAGCGGTTATCCCTCCCGAACGTAGCCAAC<br /> |rRNA<br /> |-<br /> |GTGGGAGTCCTCATCTCGAAGCAGGCTTCCCGCTTAGATG<br /> |rRNA<br /> |-<br /> |CTATCAACCCAGTCGTCTACTGGGAGCCTTAACCCCTCAAGG<br /> |rRNA<br /> |-<br /> |GCCTATTAGTACCGGTCACCTCCACACCTCACGGTGCTTCCAGATCCGGCCTATCAAC<br /> |rRNA<br /> |-<br /> |AGGACAAGCCCTCGGCCTATTAGTACCGGTCACCTCCACA<br /> |rRNA<br /> |-<br /> |CACACGCAACCCCTGCCGGGTCTCACACGTATACGGTTTG<br /> |rRNA<br /> |-<br /> |TTTCTGACTCGTCTCACGGCCGGCAGACCGTAAAAGAGAGATCCCACAACCCCGCACA<br /> |rRNA<br /> |-<br /> |TTTCGCATGTCCTTCGCCTACATCAACGGTTTCTGACTCGTCTCACGGCCGGCAGA<br /> |rRNA<br /> |-<br /> |CTTACCCTCTACGCCGGACCTTTCGCATGTCCTTCGCCTACATC<br /> |rRNA<br /> |-<br /> |GTGTCTCTCAAACGAGCCGTTGACGTTTCGACTACGGGGGTCTTACCCTCTA<br /> |rRNA<br /> |-<br /> |ATCCACCGTGCGCCCTTAAAAACTTGGCCACAGATGCTCG<br /> |rRNA<br /> |-<br /> |GATTTCTCGGGCCCCGTGCTACTTGGGTGTCTCTCAAACGAG<br /> |rRNA<br /> |-<br /> |GAATATTTAGGCTTAGCGGGTGGTCCCGCCAGATTCACACGGGATTTCTC<br /> |rRNA<br /> |-<br /> |CTCACGGTACTATCCGCTATCGGTCACCAGGGAATATTTAGGCTTAG<br /> |rRNA<br /> |-<br /> |GTTTCAGGTACTATTTCACTCCGCTCCCGCGGTACTTTTCACCATTC<br /> |rRNA<br /> |-<br /> |GATGGAGCAAGCTCCATCCCGACGCTCCCACGGCTTGTAGGCACACGGTTTCAGGTACT<br /> |rRNA<br /> |-<br /> |CACGCAGTCACGAGGATGGAGCAAGCTCCATCCCGACGCTC<br /> |rRNA<br /> |-<br /> |CTCGGGTTAACCTCGCAACACACCGCAAACTCGCAGGCTCATTCTTCAAAAGGCACGC<br /> |rRNA<br /> |-<br /> |CTACGGCTTCCCCACTCGGGTTAACCTCGCAACACACCGC<br /> |rRNA<br /> |-<br /> |GTCTTGAGCGTGCTACTAAAAACGCCCTATTCGGACTCGC<br /> |rRNA<br /> |-<br /> |CTCCCACGTCCTTCATCGGTTCCTGGTGCCAAGGCATCCAC<br /> |rRNA<br /> |-<br /> |CTTCAACCTGCCCATGGCTAGATCACTCCGCTTCGGGTCTTGAG<br /> |rRNA<br /> |-<br /> |GGTTCGGTCCTCCACGACCTCTTACAGCCGCTTCAACCTG<br /> |rRNA<br /> |-<br /> |CTAACCACAGGTCATCCCCCAGGTTTTCAACCCTGGTGGGTTCGGTCC<br /> |rRNA<br /> |-<br /> |AGAACCAGCTATCACGGAGTTTGATTGGCCTTTCACCCCTAACCACAGGTCATC<br /> |rRNA<br /> |-<br /> |CAAGAAACACACGACGCTGCACCTAAATGCATTTCGGGGAGAACCAGCTATCA<br /> |rRNA<br /> |-<br /> |TTTTAGCCTTGCGGCCGTACTCCCCAGGCGGGGCACTTAATGCGTTAGCTGCGGCAC<br /> |rRNA<br /> |-<br /> |CCGTCAATTCCTTTGAGTTTTAGCCTTGCGGCCGTACTCC<br /> |rRNA<br /> |-<br /> |AATAATCCGACCGTTCACAGCGTCCTCGCTGTTGTGTTACTTCAAAGGAA<br /> |rRNA<br /> |-<br /> |TTCTTCGCGTTGCGTCGAATTAAGCCACATGCTCCGCCGCTTGTGCGGGCCCCCGTCAA<br /> |rRNA<br /> |-<br /> |GTGTATGTCAAGCCTTGGTAAGGTTCTTCGCGTTGCGTCGAATTAAG<br /> |rRNA<br /> |-<br /> |GGGGGCACCATCTCTGATGCTTTCCGGTGTATGTCAAGCC<br /> |rRNA<br /> |-<br /> |TCACGACACGAGCTGACGACAGCCATGCACCACCTGTACACCGACCACAAGGGGGGCACC<br /> |rRNA<br /> |-<br /> |CTTAACCCAACATCTCACGACACGAGCTGACGACAGCCATG<br /> |rRNA<br /> |-<br /> |CAACACGGGACAAGGGTTGCGCTCGTTGCGGGACTTAACC<br /> |rRNA<br /> |-<br /> |GTCTCCCGTGAGTCCCCAACACCCCGAAGGGCTTGCTGGCAAC<br /> |rRNA<br /> |-<br /> |ACATAAGGGGCATGATGACTTGACGTCGTCCCCACCTTCCTCCGAGTTGACCCCG<br /> |rRNA<br /> |-<br /> |ATTGTACCGGCCATTGTAGCACGTGTGCAGCCCAAGACATAAGGGGCATGATGACTTGA<br /> |rRNA<br /> |-<br /> |TTTTGAGATTCGCTCCACCTTGCGGTATCGCAGCTCATTGTACCGGCCATTGTAGCACGT<br /> |rRNA<br /> |-<br /> |CAATCCGAACTGAGACCGGCTTTTTGAGATTCGCTCCACCTTGCGGTAT<br /> |rRNA<br /> |-<br /> |ATTACTAGCGACTCCGACTTCATGGGGTCGAGTTGCAGACCCCAATCCGAACTGAGA<br /> |rRNA<br /> |-<br /> |TATTCACCGCAGCAATGCTGATCTGCGATTACTAGCGACTCCGACTTCATG<br /> |rRNA<br /> |-<br /> |GTGTACAAGGCCCGGGAACGTATTCACCGCAGCAATGCTG<br /> |rRNA<br /> |-<br /> |GTAATCGGGTGACACCACGAGAGCGGAACAGTCGGAGGAATAA<br /> |rRNA<br /> |-<br /> |GTGTTACCGACTTTCGTGACGTGACGGGCGGTGTGTACAAG<br /> |rRNA<br /> |-<br /> |CACCTTCGACAGCTCCCTCCCACAAGGGGTTGGGCCACCGGCTTCGGGTGTTA<br /> |rRNA<br /> |-<br /> |CTACCTTGTTACGACTTCGTCCCAATCGCCAGTCCCACCTTCGACAG<br /> |rRNA<br /> |-<br /> |TCCTTAGAAAGGAGGTGATCCAGCCGCACCTTCCGGTACGGCTACCTTGTTACGA<br /> |rRNA<br /> |-<br /> |TGCAGTCGGCTATGTGCTCCTTAGAAAGGAGGTGATCCAG<br /> |rRNA<br /> |-<br /> |GATCAAACTCTCCGTGAATGTATACCCGTAATCGGGTGAC<br /> |rRNA<br /> |-<br /> |AAGTGGTTCATCGTTCGACTTGCATGTGTTAAGCACGCCGCCAGCGTTCGTCCTGA<br /> |rRNA<br /> |-<br /> |TATCAACATATCTGGCGTTGACTTTTGGCACGCTGTTGAGTTCTCAAGGAA<br /> |rRNA<br /> |-<br /> |TGTTACTCACCCGTTCGCCACTAATCCCCACCGAAGTGGTTCATCGTTCGACTT<br /> |rRNA<br /> |-<br /> |CTTGTCCCAGAGTGAAGGGCAGATTGCCCACGTGTTACTC<br /> |rRNA<br /> |-<br /> |GCAGATGCCTGCGAGGGTCAGTATCCGGTATTAGACCCCGTTTCCAGGGCTTGTCCCA<br /> |rRNA<br /> |-<br /> |ATCCTTCACCGCCGGAGCTTTCGAACCTCGCAGATGCCTGCGAGGGTCAGTATCC<br /> |rRNA<br /> |-<br /> |GATAGGCCGCGGGCTCATCCTTCACCGCCGGAGCTTTCGA<br /> |rRNA<br /> |-<br /> |GTCGCCTTGGTGAGCCATTACCTCACCAACAAGCTGATAG<br /> |rRNA<br /> |-<br /> |TAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGGCCGGTCGCCCTCTCAGGCCGGCTA<br /> |rRNA<br /> |-<br /> |ATTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTC<br /> |rRNA<br /> |-<br /> |CGTCGCTGCATCAGGCTTTCGCCCATTGTGCAATATTCCC<br /> |rRNA<br /> |-<br /> |CTTCTTCCCTGCTGAAAGAGGTTTACAACCCGAAGGCCGTCATC<br /> |rRNA<br /> |-<br /> |TTCAAAGGAACCTCAACCCGAACGGAGATCCGATCAGGTCGGGGTATCAACATAT<br /> |rRNA<br /> |-<br /> |GTAGTTAGCCGGCGCTTCTTCTGCAGGTACCGTCACTTTC<br /> |rRNA<br /> |-<br /> |ATAATTCCGGACAACGCTTGCGCCCTACGTATTACCGCGGCTGCTGGCACGTAGTTA<br /> |rRNA<br /> |-<br /> |CTTTCACAACCGACGTGACAAGCCGCCTACGAGCTCTTTACGCCCAATAATT<br /> |rRNA<br /> |-<br /> |TATCGACTGCAGACCCGGGGTTAAGCCCCGGGCTTTCACAACCGACGTGACAAG<br /> |rRNA<br /> |-<br /> |GATCTCCCCTACCGAACTCTAGCCTGCCCGTATCGACTGCAGAC<br /> |rRNA<br /> |-<br /> |CTGATATCTGCGCATTTCACCGCTACACCAGGAATTCCGATCTC<br /> |rRNA<br /> |-<br /> |CAGAGATCCGCCTTCGCCACCGGTGTTCCTCCTGATATCTG<br /> |rRNA<br /> |-<br /> |CACGCTTTCGCTCCTCAGCGTCAGTATCGGCCCAGAGATC<br /> |rRNA<br /> |-<br /> |CACCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTTC<br /> |rRNA<br /> |-<br /> |ACTTAATGCGTTAGCTGCGGCACGGACAACGTGGAATGTTGCCCACACCTAGTGCCCA<br /> |rRNA<br /> |-<br /> |GTTCGAAATGTAACCGGGCGTTTCCCCTACGCTATGACCACCGAAA<br /> |rRNA<br /> |-<br /> |CTTAGGATGGTTATAGTTACCACCGCCGTTTACTGGCGCTTAAGTTCTCA<br /> |rRNA<br /> |-<br /> |CCAAGCAAGCTTGATGTCCGACGCTCCCACGGCTTGTAGGCACACGGTTTCAGGTACTA<br /> |rRNA<br /> |-<br /> |CAGTCACGAGACACCAAGCAAGCTTGATGTCCGACGCTCC<br /> |rRNA<br /> |-<br /> |CTTGAGCGTGCTACTAAAAGCGCCCTATTCGGACTCGCTT<br /> |rRNA<br /> |-<br /> |CTACCTTGTTACGACTTCGTCCCAATCGCCAGTCCCACCTTCGACAGCTCCCT<br /> |rRNA<br /> |-<br /> |AAGGAGGTGATCCAGCCGCACCTTCCGGTACGGCTACCTTGTTACGACTTCGTC<br /> |rRNA<br /> |-<br /> |CCAGGATCAAACTCTCCGTGAATGTATACCCGTAATCGGG<br /> |rRNA<br /> |-<br /> |CTACCTTGTTACGACTTCGTCCCAATCGCCAGTCCCACCTTCG<br /> |rRNA<br /> |-<br /> |CTCTCACTTACCGGCATTCGGAGTTTGGCTAAGGTCAGTAAC<br /> |rRNA<br /> |-<br /> |CATTCGGACACCCCCGGATCAAAGCCTGGTTGACGACTCC<br /> |rRNA<br /> |-<br /> |GCTTATCCCCCACAGTCTCACTGCCGCGCTCTCACTTACC<br /> |rRNA<br /> |-<br /> |CTTAGTCGATGCTCTGGGCTGTTTCCCTCTCGACCATGGAGCTTATC<br /> |rRNA<br /> |-<br /> |CACATCCTTTCCCACTTAGCGTACGCTTAGGGGCCTTAGTCGATGCTCTG<br /> |rRNA<br /> |-<br /> |CTTCTAAGCCAACCTCCTGGTTGTCTGTGCGACTCCACATCCTTTC<br /> |rRNA<br /> |-<br /> |AATCACTAGACCAGTGAGCTATTACGCACTCTTTCAAGGGTGGCTGCTTCTAAGC<br /> |rRNA<br /> |-<br /> |CTACATTGTCGGCGCGGAATCACTAGACCAGTGAGCTATTA<br /> |rRNA<br /> |-<br /> |CGTATTGCTGCAATGACACGACTTCGGCGGTACGCTTGAG<br /> |rRNA<br /> |-<br /> |GCTCCCCTACCCATCACAGCCGGCGTTGGCCGTATTGCTG<br /> |rRNA<br /> |-<br /> |TTCTCACTCGTGAACCGTCCACAACTCGCTTCCGCGGCTGCTTCACCCGGCACACGACG<br /> |rRNA<br /> |-<br /> |GTTTGTATCGCTACTCATGCCTGCATTCTCACTCGTGAACCGTCCACAAC<br /> |rRNA<br /> |-<br /> |CTTAGTCAATCGGCGCAAACGTTTCTCACGTTTGTATCGCTACTCATG<br /> |rRNA<br /> |-<br /> |GACTTACCCTGGGCAGATCAGCTTGACCCAGGAACCCTTAGTCAATC<br /> |rRNA<br /> |-<br /> |TCCACACTGCCTATGTGTTCAGCAGCGGGTGACAGCCCATGACGACTGCCGGGTTT<br /> |rRNA<br /> |-<br /> |TTATCCATCGACTACGCCTGTCGGCCTCGCCTTAGGTCCCGACTTACCCTGGGCA<br /> |rRNA<br /> |-<br /> |GTACCGGAATATCAACCGGTTATCCATCGACTACGCCTGTC<br /> |rRNA<br /> |-<br /> |GGCCTTAGCATCACGATGCTCGATGTTTGACGCTTCACAG<br /> |rRNA<br /> |-<br /> |CTACTAACCGCTTGGTTCGGCGGCTCCACCACTCCCCTCAACTCCGAAGAGATCA<br /> |rRNA<br /> |-<br /> |GATGGACTACCTTCCTGCGTCACCCCATCACTCACCTACTAAC<br /> |rRNA<br /> |-<br /> |CCGGCCTACACCCTTACCCCGGGACAACCACCGCCCGGGATGGACTACC<br /> |rRNA<br /> |-<br /> |CAAGGCATCCACCGTGCGCCCTTAAAAACTTGGCCACAGA<br /> |rRNA<br /> |-<br /> |CAAGGTGTGCGGATTTACCTACACACCGGCCTACACCCTTAC<br /> |rRNA<br /> |-<br /> |CCACAATCGGCTCGGCATCAGGTCTCAGCCACAAGGTGTG<br /> |rRNA<br /> |-<br /> |CTAGAGGCTTTTCTCGACAGCATAGGATCATCCACTTCACCACAATC<br /> |rRNA<br /> |-<br /> |TTTAGGGTACGGGCCGCCATGAAACTCGCTAGAGGCTTTTCTC<br /> |rRNA<br /> |-<br /> |CTCGGTATTCTCTACCTGACCACCTGAGTCGGTTTAGGGTAC<br /> |rRNA<br /> |-<br /> |GGCATTTTGCCGAGTTCCTTAACCATAGTTCACCCGAACG<br /> |rRNA<br /> |-<br /> |CAGCTCAGAGTGCAAAACTCGTCACCAGGTGTGGCCCCCCTTCTCCCGAAGTTA<br /> |rRNA<br /> |-<br /> |TTGTTTTCTCTTCCTGCGGGTACTGAGATGTTTCACTTCCCCGCGTTCCCTCCACA<br /> |rRNA<br /> |-<br /> |TGTGTTTTTAGTAAACAGTCGCTTCTCGCTGGTCTCTGCGGCCACCCCCAGCTCAGA<br /> |rRNA<br /> |-<br /> |TATACATCGCCTTACGGCTTCGCACGGACCTGTGTTTTTAGTAAACAGTCGCTTCT<br /> |rRNA<br /> |-<br /> |GCAGGCGTCAGTCCGTATACATCGCCTTACGGCTTCGCAC<br /> |rRNA<br /> |-<br /> |GAAATGTGACTAACCGGTCCCCTTAACGTTCCAGCACCGGGCAGGC<br /> |rRNA<br /> |-<br /> |GTTTACTGGCGCTTAAGTTCTCAGCTTCGCCACACCGAAATGTGACTAAC<br /> |rRNA<br /> |-<br /> |GACAAGGAATTTCGCTACCTTAGGATGGTTATAGTTACCACCGCCGTTTA<br /> |rRNA<br /> |-<br /> |AAGTCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTA<br /> |rRNA<br /> |-<br /> |CTATGGTTGAGACAGTCGAGAAGTCGTTACGCCATTCGTG<br /> |rRNA<br /> |-<br /> |CTGCGCGAAACGAGCATCTTTACTCGTAGTGCAATTTCAC<br /> |rRNA<br /> |-<br /> |CAATATCAAACTGTAGTAAAGGTCCCGGGGTCTTTCCGTCCTGCTGCGCGAAA<br /> |rRNA<br /> |-<br /> |CAGCTATCCTACACAAGCCGAACCGAACACCAATATCAAACTGTAGTAAA<br /> |rRNA<br /> |-<br /> |CACACTGGCGTGCGAGCTTCAAAGTCTCCCAGCTATCCTACACAAG<br /> |rRNA<br /> |-<br /> |TTAGACATCCAGCACGACCAGACTGGTATTTCAACGACGACTCCACCCACACTGGCGTG<br /> |rRNA<br /> |-<br /> |GTTTCGCTCGCCACTACTCCCGGAATCACGGTTGTTTTCTCTTC<br /> |rRNA<br /> |-<br /> |GATCACGGACCCAGGTTAGACATCCAGCACGACCAGACTG<br /> |rRNA<br /> |-<br /> |CAGTTAAACTACCCATCAGACACTGTCCCTGATCCGGATCAC<br /> |rRNA<br /> |-<br /> |GAACCTTTGGGCGCCTCCGTTACTCTTTAGGAGGCAACCG<br /> |rRNA<br /> |-<br /> |CTTGTGCACTTACACTCAACACCTGATTGCCAACCAGGCTGAGGGAACCTTT<br /> |rRNA<br /> |-<br /> |GACCCGTCGGTCTCACAGTCAAGCTCCCTTGTGCACTTAC<br /> |rRNA<br /> |-<br /> |CTTCCACAAGCCACCGCCGGATCACTAGTCCCGACTTTCGTCCCTG<br /> |rRNA<br /> |-<br /> |GTACCTTTTATCCGTTGAGCGACGGCGCTTCCACAAGCCAC<br /> |rRNA<br /> |-<br /> |CAAACCATCCCGTCGATATGGACTCTTGGGGAAGATCAGCCTGTTATC<br /> |rRNA<br /> |-<br /> |CCAGGATGCGACGAGCCGACATCGAGGTGCCAAACCATCC<br /> |rRNA<br /> |-<br /> |GTCTCACACGTATACGGTTTGGCCTCATCCGGTTTCGCTC<br /> |rRNA<br /> |-<br /> |CTTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTCCAG<br /> |rRNA<br /> |-<br /> |GAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCGCTTTAATG<br /> |rRNA<br /> |-<br /> |CTTCTCAAGACTCCTACGCGCACAGCGGATAGGGACCGAAC<br /> |rRNA<br /> |-<br /> |CACACCAGAGGTTCGTCCGTCCCGGTCCTCTCGTACTAGG<br /> |rRNA<br /> |-<br /> |TTAGATGCTTTCAGCGGTTATCCCTCCCGAACGTAGCCAACCAGCCATGCCCTTGGCA<br /> |rRNA<br /> |-<br /> |CTCATCTCGAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATC<br /> |rRNA<br /> |-<br /> |CAGTCGTCTACTGGGAGCCTTAACCCCTCAAGGGGGTGGGAGTCCTCATCTCGAA<br /> |rRNA<br /> |-<br /> |CTTCCAGATCCGGCCTATCAACCCAGTCGTCTACTGGGAG<br /> |rRNA<br /> |-<br /> |CAGACCGTAAAAGAGAGATCCCACAACCCCGCACACGCAACCCCTGCCGGGTCTCA<br /> |rRNA<br /> |-<br /> |TACATCAACGGTTTCTGACTCGTCTCACGGCCGGCAGACCGTAAAAGAGAGAT<br /> |rRNA<br /> |-<br /> |CTTTCGCATGTCCTTCGCCTACATCAACGGTTTCTGACTC<br /> |rRNA<br /> |-<br /> |ACTACGGGGGTCTTACCCTCTACGCCGGACCTTTCGCATG<br /> |rRNA<br /> |-<br /> |CTACTTGGGTGTCTCTCAAACGAGCCGTTGACGTTTCGACTAC<br /> |rRNA<br /> |-<br /> |GCCAGATTCACACGGGATTTCTCGGGCCCCGTGCTACTTG<br /> |rRNA<br /> |-<br /> |TATCCGCTATCGGTCACCAGGGAATATTTAGGCTTAGCGGGTGGTCCCGCCAGATT<br /> |rRNA<br /> |-<br /> |GTACTTTTCACCATTCCCTCACGGTACTATCCGCTATCGGTCAC<br /> |rRNA<br /> |-<br /> |CTTGTAGGCACACGGTTTCAGGTACTATTTCACTCCGCTCCC<br /> |rRNA<br /> |-<br /> |GGGCCTATCGTGGCCTCCCACGTCCTTCATCGGTTCCTGG<br /> |rRNA<br /> |-<br /> |CACGCAGTCACGAGGATGGAGCAAGCTCCATCCCGACGCTCCCACGGCTTGTAGGCA<br /> |rRNA<br /> |-<br /> |CATTCTTCAAAAGGCACGCAGTCACGAGGATGGAGCAAGC<br /> |rRNA<br /> |-<br /> |TACGGCTTCCCCACTCGGGTTAACCTCGCAACACACCGCAAACTCGCAGGCTCAT<br /> |rRNA<br /> |-<br /> |TCGGACTCGCTTTCGCTACGGCTTCCCCACTCGGGTTAAC<br /> |rRNA<br /> |-<br /> |ATCACTCCGCTTCGGGTCTTGAGCGTGCTACTAAAAACGC<br /> |rRNA<br /> |-<br /> |CTCTTACAGCCGCTTCAACCTGCCCATGGCTAGATCACTC<br /> |rRNA<br /> |-<br /> |GTTTTCAACCCTGGTGGGTTCGGTCCTCCACGACCTCTTAC<br /> |rRNA<br /> |-<br /> |GTTTGATTGGCCTTTCACCCCTAACCACAGGTCATCCCCCAGGTTTTCAACC<br /> |rRNA<br /> |-<br /> |GGAGAACCAGCTATCACGGAGTTTGATTGGCCTTTCACCC<br /> |rRNA<br /> |-<br /> |TATCCAGTGCTCTACCTCCGGCAAGAAACACACGACGCTGCACCTAAATGCATTT<br /> |rRNA<br /> |-<br /> |GAGTTTGGCTAAGGTCAGTAACCCGGTAGGGCCCATCGCCTATCCAGTGCTCTA<br /> |rRNA<br /> |-<br /> |CTGTAAGGCTTAGCTTCCGGGTTCGAAATGTAACCGGGCGTTTCCCCTAC<br /> |rRNA<br /> |-<br /> |GTTCGGCGGCGTCCTACTCTCCCACAGGGTCCCCCCTGCAGTACCATCGGCGCTGTA<br /> |rRNA<br /> |-<br /> |GTATTAGACCCCGTTTCCAGGGCTTGTCCCAGAGTGAAGGGCAGATT<br /> |rRNA<br /> |-<br /> |GAGCTTTCGAACCTCGCAGATGCCTGCGAGGGTCAGTATCCGGTATTAGACCCCGTT<br /> |rRNA<br /> |-<br /> |CATCCTTCACCGCCGGAGCTTTCGAACCTCGCAGATGCCT<br /> |rRNA<br /> |-<br /> |CACCGAAGTGGTTCATCGTTCGACTTGCATGTGTTAAGCAC<br /> |rRNA<br /> |-<br /> |TTCGACTTGCATGTGTTAAGCACGCCGCCAGCGTTCGTCCTGAGCCAGGATCAAACTCT<br /> |rRNA<br /> |-<br /> |CAGATTGCCCACGTGTTACTCACCCGTTCGCCACTAATCC<br /> |rRNA<br /> |-<br /> |GCGGATTTACCTACCTGACGTCCTACACCCTTACCCCGGGACAACCACCGC<br /> |rRNA<br /> |-<br /> |CATCAGGTCTCAGACTCATGTCAGGCGGATTTACCTACCTGAC<br /> |rRNA<br /> |-<br /> |CTATTAGTACCGGTCACCTCCACACCTCACGGTGCTTCCAGATCCGGCCTATCAACC<br /> |rRNA<br /> |-<br /> |GGACAAGCCCTCGGCCTATTAGTACCGGTCACCTCCACAC<br /> |rRNA<br /> |-<br /> |CCGGGTTAACCTCGCAACACACCGCAAACTCGCAGGCTCATTCTTCAAAAGGCACGC<br /> |rRNA<br /> |-<br /> |GGTCTTGAGCGTGCTACTAAAACGCCCTATTCGGACTCGC<br /> |rRNA<br /> |-<br /> |TTCGGCGGCGTCCTACTCTCCCACAGGGTCCCCCCTGCAGTACCATCGGCGCTGTAA<br /> |rRNA<br /> |-<br /> |CCGAAGCCTCCGGCGGTCTGCTTTCTGTGGCACTGTCCCG<br /> |SCOs01<br /> |-<br /> |CTGGGCACTGGTGGTCTCTTACACCACCGTTTCACCCTTAC<br /> |SCOs01<br /> |-<br /> |TTCCTTCTTGACCTTGCTCCGGGTGGGGTTTACCTAGCTGCCCAGGTCGCCCTG<br /> |SCOs01<br /> |-<br /> |CTCGAACGTCCGCGCAGAAGCACCGGGGTGCTTCCTTCTTGACCTTGCTC<br /> |SCOs01<br /> |-<br /> |CTCCAGCGGTCTACCCGCGGACTCGGGCGGGCAGCCCTCGAACGTCCGCGCAGAAG<br /> |SCOs01<br /> |-<br /> |CATCCATCTAGGACCGGCGTTGCCACCGGCCTCCAGCGGTCTAC<br /> |SCOs01<br /> |-<br /> |CTGTGGAGCCCGGACGTTCCTCGGGAAGTCCCGTGAGGGACTC<br /> |SCOs01<br /> |-<br /> |GTTCTGTTCCGACGGGGCCTCGCGGTCCCGTCGGCGACGGCCATCCAT<br /> |SCOs01<br /> |-<br /> |CAGACGAGTCGGGCTGTACGCCGGGTTCTGTTCCGACGGG<br /> |SCOs01<br /> |-<br /> |GTCTGCTTTCTGTGGCACTGTCCCGCGGGTCACCCCGGGTGGCCGTTAGCCATCAC<br /> |SCOs01<br /> |-<br /> |CTTAGTAGGAGGCTCGCTTCGCTACCTGAGATCAGGCAGCGAGGGCGAAGG<br /> |SCOs02<br /> |-<br /> |GAACAGCCCCGGGCTGACACTCCCTTAGTAGGAGGCTCGCTTC<br /> |SCOs02<br /> |-<br /> |GATCAAGGTTTAGTCCCCTAGCTGATGCCAGGATCCGGGTC<br /> |SCOs02<br /> |-<br /> |CAGTCACTGTTTGGTTTCCCTCTTCACCCCGTGACCGGGATCAA<br /> |SCOs02<br /> |-<br /> |GAGATCACGCGGACAAGTCTCCGACGGGCCCAGTCACTGT<br /> |SCOs02<br /> |-<br /> |CTTCCCCTGCTTCGACAGCCGCTGTCGAAACCGATCATCC<br /> |SCOs02<br /> |-<br /> |AATCAGGGCTTCTCCGTGTGCAGTCCGCTTCGATTTTCTCGGCCCCGGAGATCACG<br /> |SCOs02<br /> |-<br /> |GAATCGAACCCGCGTCCAACGGTGCGGAATCAGGGCTTCTC<br /> |SCOs02<br /> |-<br /> |TTTTTCGGCCTGTGGTTTACGAGATCATGGCCGCTTCCTC<br /> |SCOs02<br /> |-<br /> |CTGCTGGGAGGAATCGCGCTTGGTGTTGGCGATTATTTTT<br /> |SCOs02<br /> |-<br /> |CACGCACCCGCCAGAGCCGACCTACCCTTGCTGCCTTCCG<br /> |SCOs03<br /> |}<br /> <br /> ==Sign off==<br /> <br /> Page created by R. T. Evans, University of Brighton.<br /> stonybonytony@gmail.com</div> RTEvans https://actinobase.org/index.php?title=RRNA_depletion_with_Streptomyces_coelicolor_A3(2)_specific_probes&diff=2197 RRNA depletion with Streptomyces coelicolor A3(2) specific probes 2023-11-01T16:50:52Z <p>RTEvans: </p> <hr /> <div>==Organisms==<br /> <br /> This protocol has been tested with &lt;em&gt;[[Streptomyces coelicolor]]&lt;/em&gt; A3(2) MT1110<br /> <br /> ==Description==<br /> <br /> rRNA depletion using commercial kits targeting generic bacterial sequences is not 100% efficient in the High GC organism &lt;em&gt;S. coelicolor&lt;/em&gt;. <br /> <br /> Depletion using the NEBNext RNA Depletion Core Reagent Set with RNA Sample Purification Beads (https://www.neb.com/en-gb/products/e7870-nebnext-rna-depletion-core-reagent-set-with-rna-sample-purification-beads#Product%20Information) and custom DNA probes designed for &lt;em&gt;S. coelicolor&lt;/em&gt; is more effective than the QIAseq FastSelect 5S/16S/23S kit (Qiagen #335927); NEBNext rRNA Depletion Kit (Bacteria) (NEB #E7850); and Illumina Ribo-zero rRNA Removal Kit (Bacteria) (Illumina #MRZMB12424, discontinued). The Illumina kit tested was subsequently replaced by the Illumina Ribo-Zero Plus rRNA Depletion Kit (Illumina #20040526). <br /> <br /> In addition, three abundant non-mRNA RNAs can also be depleted using the custom probes (Table 1).<br /> <br /> '''Table 1.''' Abundant non-mRNA RNAs in &lt;em&gt;S. coelicolor&lt;/em&gt;.<br /> <br /> [[File: rRNA_depletion_table_1.png]]<br /> <br /> ==Materials==<br /> <br /> Single stranded DNA probes at 2µM concentration in IDTE pH 7.5 (10mM Tris-HCl / 0.1mM EDTA). Integrated DNA Technologies can produce these from their 25nmole DNA oligo pool product. Probes are listed in Table 2 in the Notes section below.<br /> <br /> NEBNext RNA Depletion Core Reagent Set with RNA Sample Purification Beads (https://www.neb.com/en-gb/products/e7870-nebnext-rna-depletion-core-reagent-set-with-rna-sample-purification-beads#Product%20Information).<br /> <br /> ==Protocols==<br /> <br /> As per manufacturer’s instructions.<br /> <br /> ==Notes==<br /> <br /> The following histograms illustrate effectiveness of rRNA depletion using various methods. Histograms show percentage of bases aligned to NCBI reference sequence NC_003888.3 using Hisat2 program classified as follows - Coding = RNA aligned to coding sequences; Transcribed not translated = RNA aligned to rRNA, tRNA and sRNA; Intergenic = RNA aligned to 5' or 3' UTRs or between annotated sequences.<br /> <br /> '''QIAseq FastSelect 5S/16S/23S kit'''<br /> <br /> [[File: PanelA.png]] <br /> <br /> '''NEBNext rRNA Depletion Kit (Bacteria)'''<br /> <br /> [[File: PanelB.png]]<br /> <br /> '''Illumina Ribo-zero rRNA Removal Kit (Bacteria)'''<br /> <br /> [[File: PanelC.png]] <br /> <br /> '''rRNA depletion with &lt;em&gt;S. coelicolor&lt;/em&gt; specific probes<br /> <br /> [[File: PanelD.png]]<br /> <br /> <br /> '''Table 2''' List of probes<br /> <br /> {| class=&quot;wikitable&quot;<br /> !Sequence<br /> !Target<br /> |-<br /> |CAAGTCCTTGCGCGACGCTCCCACGGCTTGTAGGCACACGGTTTCAGGTACTATTT<br /> |rRNA<br /> |-<br /> |CAGTCACGACGCAAGGACAAGTCCTTGCGCGACGCTCCCAC<br /> |rRNA<br /> |-<br /> |ACCTCGCAACACACCGCAAACTCGCAGGCTCATTCTTCAAAAGGCACGCAGTCACGACG<br /> |rRNA<br /> |-<br /> |CTACGGCTTCCCCACCCGGGTTAACCTCGCAACACACCGC<br /> |rRNA<br /> |-<br /> |TTAACCCAACATCTCACGACACGAGCTGACGACAGCCATGCACCACCTGTACACCGA<br /> |rRNA<br /> |-<br /> |CGCTCGTTGCGGGACTTAACCCAACATCTCACGACACGAG<br /> |rRNA<br /> |-<br /> |CAACACCCCGAAGGGCTTGCTGGCAACACGGGACAAGGGTTG<br /> |rRNA<br /> |-<br /> |CACCTTCCTCCGAGTTGACCCCGGCGGTCTCCCGTGAGTC<br /> |rRNA<br /> |-<br /> |GACATAAGGGGCATGATGACTTGACGTCGTCCCCACCTTC<br /> |rRNA<br /> |-<br /> |GTATCGCAGCTCATTGTACCGGCCATTGTAGCACGTGTGCAGCCCAAGACATAA<br /> |rRNA<br /> |-<br /> |TTTTGAGATTCGCTCCACCTTGCGGTATCGCAGCTCATTGTAC<br /> |rRNA<br /> |-<br /> |CAGACCCCAATCCGAACTGAGACCGGCTTTTTGAGATTCG<br /> |rRNA<br /> |-<br /> |AATGCTGATCTGCGATTACTAGCGACTCCGACTTCATGGGGTCGAGTTGCAGAC<br /> |rRNA<br /> |-<br /> |GAACGTATTCACCGCAGCAATGCTGATCTGCGATTACTAG<br /> |rRNA<br /> |-<br /> |TACCGACTTTCGTGACGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTC<br /> |rRNA<br /> |-<br /> |GCCACCGGCTTCGGGTGTTACCGACTTTCGTGACGTGACG<br /> |rRNA<br /> |-<br /> |TTCGTCCCAATCGCCAGTCCCACCTTCGACAGCTCCCTCCCACAAGGGGTTGGG<br /> |rRNA<br /> |-<br /> |CGCACCTTCCGGTACGGCTACCTTGTTACGACTTCGTCCCAATCGCCAGTCCCA<br /> |rRNA<br /> |-<br /> |TTCGACCCTCGCAGATGCCTGCGAGGGTCAGTATCCGGTATTAGACCCCGTTTCCAG<br /> |rRNA<br /> |-<br /> |GAAAGGAGGTGATCCAGCCGCACCTTCCGGTACGGCTACC<br /> |rRNA<br /> |-<br /> |TTCACCGCCGGAGCTTTCGACCCTCGCAGATGCCTGCGAG<br /> |rRNA<br /> |-<br /> |CATTACCTCACCAACAAGCTGATAGGCCGCGGGCTCATCCTTC<br /> |rRNA<br /> |-<br /> |CTCTCAGGCCGGCTACCCGTCGTCGCCTTGGTGAGCCATTAC<br /> |rRNA<br /> |-<br /> |GTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGGCCGGTCG<br /> |rRNA<br /> |-<br /> |TGCATCAGGCTTTCGCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCT<br /> |rRNA<br /> |-<br /> |TGCTGAAAGAGGTTTACAACCCGAAGGCCGTCATCCCTCACGCGGCGTCGCTGCATCA<br /> |rRNA<br /> |-<br /> |TTCTTCTGCAGGTACCGTCACTTTCGCTTCTTCCCTGCTGAAAGAGGTTTACAA<br /> |rRNA<br /> |-<br /> |CTACGTATTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTCTG<br /> |rRNA<br /> |-<br /> |CTCTTTACGCCCAATAATTCCGGACAACGCTTGCGCCCTACGTATTAC<br /> |rRNA<br /> |-<br /> |GGGCTTTCACAACCGACGTGACAAGCCGCCTACGAGCTCT<br /> |rRNA<br /> |-<br /> |CTACCGAACTCTAGCCTGCCCGTATCGACTGCAGACCCGGGGTTAAGCCCCG<br /> |rRNA<br /> |-<br /> |ATATCTGCGCATTTCACCGCTACACCAGGAATTCCGATCTCCCCTACCGAACTCTAG<br /> |rRNA<br /> |-<br /> |CGGTGTTCCTCCTGATATCTGCGCATTTCACCGCTACACC<br /> |rRNA<br /> |-<br /> |ACGCTTTCGCTCCTCAGCGTCAGTATCGGCCCAGAGATCCGCCTTCGCCACCGGTGT<br /> |rRNA<br /> |-<br /> |GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCG<br /> |rRNA<br /> |-<br /> |ACAACGTGGAATGTTGCCCACACCTAGTGCCCACCGTTTACGGCGTGGACTACCA<br /> |rRNA<br /> |-<br /> |CTTAATGCGTTAGCTGCGGCACGGACAACGTGGAATGTTG<br /> |rRNA<br /> |-<br /> |TCAATTCCTTTGAGTTTTAGCCTTGCGGCCGTACTCCCCAGGCGGGGCACTTAA<br /> |rRNA<br /> |-<br /> |ATTAAGCCACATGCTCCGCCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTTA<br /> |rRNA<br /> |-<br /> |TCAAGCCTTGGTAAGGTTCTTCGCGTTGCGTCGAATTAAGCCACATGCTCCGCCGCTT<br /> |rRNA<br /> |-<br /> |CATCTCTGATGCTTTCCGGTGTATGTCAAGCCTTGGTAAGGTTCTTC<br /> |rRNA<br /> |-<br /> |ACAGCCATGCACCACCTGTACACCGACCACAAGGGGGGCACCATCTCTGATGCTTTCC<br /> |rRNA<br /> |-<br /> |TGTAACCGGGCGTTTCCCCTACGCTATGACCACCGAAACC<br /> |rRNA<br /> |-<br /> |CAGTACCATCGGCGCTGTAAGGCTTAGCTTCCGGGTTCGAAATGTAAC<br /> |rRNA<br /> |-<br /> |GTTCGGCGGCGTCCTACTCTCCCACAGGGTCCCCCCTGCA<br /> |rRNA<br /> |-<br /> |CACTCCCCTCAACTCCGAAGAGATCAGGGCGGCTTCACGG<br /> |rRNA<br /> |-<br /> |CCATCACTCACCTACTAACCGCTTGGTTCGGCGGCTCCAC<br /> |rRNA<br /> |-<br /> |GTCCTTCATCGGTTCCTGGTGCCAAGGCATCCACCGTGCGCCCTTAAAAACTT<br /> |rRNA<br /> |-<br /> |CTTGTAGGCACACGGTTTCAGGTACTATTTCACTCCGCTCCCG<br /> |rRNA<br /> |-<br /> |CACGCAGTCACGAGACACCAAGCAAGCTTGATGTCCGACGCTCCCACGGCTTGTAGGCAC<br /> |rRNA<br /> |-<br /> |TCTTCAAAAGGCACGCAGTCACGAGACACCAAGCAAGCTT<br /> |rRNA<br /> |-<br /> |CACCCGGGTTAACCTCGCAACACACTGCAAACTCGCAGGC<br /> |rRNA<br /> |-<br /> |GCGTGCTACTAAAACGCCCTATTCGGACTCGCTTTCGCTACGGCTTCC<br /> |rRNA<br /> |-<br /> |GATCACTCCGCTTCGGGTCTTGAGCGTGCTACTAAAACGC<br /> |rRNA<br /> |-<br /> |TCGCAGATGCCTGCAAGGGTCAGTATCCGGTATTAGACCCCGTTT<br /> |rRNA<br /> |-<br /> |TCATCCTTCACCGCCGCAGCTTTCGAACCTCGCAGATGCC<br /> |rRNA<br /> |-<br /> |ATGTAACCGGGCGTTTCCCCTACGCTATGACCACCGAAAC<br /> |rRNA<br /> |-<br /> |CGTCCTACTCTCCCACAGGGTCCCCCCTGCAGTACCATCG<br /> |rRNA<br /> |-<br /> |CATCGCCTATCCAGTGCTCTACCTCCGGCAAGAAACACAC<br /> |rRNA<br /> |-<br /> |CATTCGGAGTTTGGCTAAGGTCAGTAACCCGGTAGGGCCCATCG<br /> |rRNA<br /> |-<br /> |CAGTCTCACTGCCGCGCTCTCACTTACCGGCATTCGGAGTTTG<br /> |rRNA<br /> |-<br /> |ATGCTCTGGGCTGTTTCCCTCTCGACCATGGAGCTTATCCCCCACAGTCTCACTG<br /> |rRNA<br /> |-<br /> |CTTAGGGGCCTTAGTCGATGCTCTGGGCTGTTTCCCTCTCG<br /> |rRNA<br /> |-<br /> |GTTGTCTGTGCGACTCCACATCCTTTCCCACTTAGCGTACGCTTAG<br /> |rRNA<br /> |-<br /> |TATTACGCACTCTTTCAAGGGTGGCTGCTTCTAAGCCAACCTCCTGGTTGTCTGT<br /> |rRNA<br /> |-<br /> |GATCAAAGCCTGGTTGACGACTCCCCGGGGCCTATCGTGG<br /> |rRNA<br /> |-<br /> |CGGAATCACTAGACCAGTGAGCTATTACGCACTCTTTCAAGG<br /> |rRNA<br /> |-<br /> |TATTGCTGCAATGACACGACTTCGGCGGTACGCTTGAGCCCCGCTACATTGTCG<br /> |rRNA<br /> |-<br /> |CATCACAGCCGGCGTTGGCCGTATTGCTGCAATGACACGACTTC<br /> |rRNA<br /> |-<br /> |TCCACAACTCGCTTCCGCGGCTGCTTCACCCGGCACACGACGCTCCCCTACCCATCACA<br /> |rRNA<br /> |-<br /> |TACTCATGCCTGCATTCTCACTCGTGAACCGTCCACAACTCGCTTCCGCGGCTGCTT<br /> |rRNA<br /> |-<br /> |CAAACGTTTCTCACGTTTGTATCGCTACTCATGCCTGCATTCTCACTC<br /> |rRNA<br /> |-<br /> |CAGGAACCCTTAGTCAATCGGCGCAAACGTTTCTCACGTTT<br /> |rRNA<br /> |-<br /> |CTTAGGTCCCGACTTACCCTGGGCAGATCAGCTTGACCCAG<br /> |rRNA<br /> |-<br /> |GAATATCAACCGGTTATCCATCGACTACGCCTGTCGGCCTCGCCTTAGGTC<br /> |rRNA<br /> |-<br /> |TTAGCATCACGATGCTCGATGTTTGACGCTTCACAGCGGGTACCGGAATATCAA<br /> |rRNA<br /> |-<br /> |GAAGAGATCAGGGCGGCTTCACGGCCTTAGCATCACGATG<br /> |rRNA<br /> |-<br /> |CATCACTCACCTACTAACCGCTTGGTTCGGCGGCTCCACC<br /> |rRNA<br /> |-<br /> |TGTGTTCAGCAGCGGGTGACAGCCCATGACGACTGCCGGGTTTCCCCATTCGGACACCC<br /> |rRNA<br /> |-<br /> |GACAACCACCGCCCGGGATGGACTACCTTCCTGCGTCACCCCAT<br /> |rRNA<br /> |-<br /> |CAGATGCTCGCGTCCACTGTGCAGTTCTCAAACAACGACC<br /> |rRNA<br /> |-<br /> |TGCGGATTTACCTACACACCGGCCTACACCCTTACCCCGGGACAACCACCG<br /> |rRNA<br /> |-<br /> |CATCAGGTCTCAGCCACAAGGTGTGCGGATTTACCTACAC<br /> |rRNA<br /> |-<br /> |TTTTCTCGACAGCATAGGATCATCCACTTCACCACAATCGGCTCGGCATCAGGTCT<br /> |rRNA<br /> |-<br /> |CCATGAAACTCGCTAGAGGCTTTTCTCGACAGCATAGGATCATC<br /> |rRNA<br /> |-<br /> |TATTCTCTACCTGACCACCTGAGTCGGTTTAGGGTACGGGCCGCCATGAAACT<br /> |rRNA<br /> |-<br /> |GAGTTCCTTAACCATAGTTCACCCGAACGCCTCGGTATTCTCTACCTGAC<br /> |rRNA<br /> |-<br /> |CTTCTCCCGAAGTTACGGGGGCATTTTGCCGAGTTCCTTAACCA<br /> |rRNA<br /> |-<br /> |CACCCCCAGCTCAGAGTGCAAAACTCGTCACCAGGTGTGG<br /> |rRNA<br /> |-<br /> |GTGTTTTTAGTAAACAGTCGCTTCTCGCTGGTCTCTGCGG<br /> |rRNA<br /> |-<br /> |GTTCCCTCCACACTGCCTATGTGTTCAGCAGCGGGTGACAG<br /> |rRNA<br /> |-<br /> |GTATACATCGCCTTACGGCTTCGCACGGACCTGTGTTTTTA<br /> |rRNA<br /> |-<br /> |CTTAACGTTCCAGCACCGGGCAGGCGTCAGTCCGTATACATC<br /> |rRNA<br /> |-<br /> |TAAGTTCTCAGCTTCGCCACACCGAAATGTGACTAACCGGTCCCCTTAACGTT<br /> |rRNA<br /> |-<br /> |TTAGGATGGTTATAGTTACCACCGCCGTTTACTGGCGCTTAAGTTCTCAG<br /> |rRNA<br /> |-<br /> |GTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGATGGTTATAGT<br /> |rRNA<br /> |-<br /> |TATGGTTGAGACAGTCGAGAAGTCGTTACGCCATTCGTGCAGGTCGGAACTTACC<br /> |rRNA<br /> |-<br /> |GAAACGAGCATCTTTACTCGTAGTGCAATTTCACCGGGCCTATGGTTGAG<br /> |rRNA<br /> |-<br /> |GGTCTTTCCGTCCTGCTGCGCGAAACGAGCATCTTTACTC<br /> |rRNA<br /> |-<br /> |GAACCGAACACCAATATCAAACTGTAGTAAAGGTCCCGGGGTCTTTC<br /> |rRNA<br /> |-<br /> |CTTCAAAGTCTCCCAGCTATCCTACACAAGCCGAACCGAACACCAATA<br /> |rRNA<br /> |-<br /> |AGACTGGTATTTCAACGACGACTCCACCCACACTGGCGTGCGAGCTTCAAAGTCT<br /> |rRNA<br /> |-<br /> |GTTAGACATCCAGCACGACCAGACTGGTATTTCAACGACGACTC<br /> |rRNA<br /> |-<br /> |TTAAACTACCCATCAGACACTGTCCCTGATCCGGATCACGGACCCAGGTTAGACAT<br /> |rRNA<br /> |-<br /> |GAATCACGGTTGTTTTCTCTTCCTGCGGGTACTGAGATGTTTCACTTC<br /> |rRNA<br /> |-<br /> |GTTACTCTTTAGGAGGCAACCGCCCCAGTTAAACTACCCATCAGACACTG<br /> |rRNA<br /> |-<br /> |ACTCAACACCTGATTGCCAACCAGGCTGAGGGAACCTTTGGGCGCCTCCGTTACT<br /> |rRNA<br /> |-<br /> |GTCTCACAGTCAAGCTCCCTTGTGCACTTACACTCAACACCTGATTG<br /> |rRNA<br /> |-<br /> |GACTTTCGTCCCTGCTCGACCCGTCGGTCTCACAGTCAAG<br /> |rRNA<br /> |-<br /> |TACCTTTTATCCGTTGAGCGACGGCGCTTCCACAAGCCACCGCCGGATCACTAGTC<br /> |rRNA<br /> |-<br /> |GTCGATATGGACTCTTGGGGAAGATCAGCCTGTTATCCCCGGGGTACCTTTTAT<br /> |rRNA<br /> |-<br /> |GACATCGAGGTGCCAAACCATCCCGTCGATATGGACTCTTG<br /> |rRNA<br /> |-<br /> |TTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTCCAGCCCCAGGATGCGACGA<br /> |rRNA<br /> |-<br /> |TCACGACGTTCTAAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACC<br /> |rRNA<br /> |-<br /> |GATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTC<br /> |rRNA<br /> |-<br /> |GTCCTCTCGTACTAGGGACAGCCCTTCTCAAGACTCCTAC<br /> |rRNA<br /> |-<br /> |TCTCACACGTATACGGTTTGGCCTCATCCGGTTTCGCTCGCCACTACTCCCGGAAT<br /> |rRNA<br /> |-<br /> |CATGCCCTTGGCAGGACAACTGGCACACCAGAGGTTCGTC<br /> |rRNA<br /> |-<br /> |CTTAGATGCTTTCAGCGGTTATCCCTCCCGAACGTAGCCAAC<br /> |rRNA<br /> |-<br /> |GTGGGAGTCCTCATCTCGAAGCAGGCTTCCCGCTTAGATG<br /> |rRNA<br /> |-<br /> |CTATCAACCCAGTCGTCTACTGGGAGCCTTAACCCCTCAAGG<br /> |rRNA<br /> |-<br /> |GCCTATTAGTACCGGTCACCTCCACACCTCACGGTGCTTCCAGATCCGGCCTATCAAC<br /> |rRNA<br /> |-<br /> |AGGACAAGCCCTCGGCCTATTAGTACCGGTCACCTCCACA<br /> |rRNA<br /> |-<br /> |CACACGCAACCCCTGCCGGGTCTCACACGTATACGGTTTG<br /> |rRNA<br /> |-<br /> |TTTCTGACTCGTCTCACGGCCGGCAGACCGTAAAAGAGAGATCCCACAACCCCGCACA<br /> |rRNA<br /> |-<br /> |TTTCGCATGTCCTTCGCCTACATCAACGGTTTCTGACTCGTCTCACGGCCGGCAGA<br /> |rRNA<br /> |-<br /> |CTTACCCTCTACGCCGGACCTTTCGCATGTCCTTCGCCTACATC<br /> |rRNA<br /> |-<br /> |GTGTCTCTCAAACGAGCCGTTGACGTTTCGACTACGGGGGTCTTACCCTCTA<br /> |rRNA<br /> |-<br /> |ATCCACCGTGCGCCCTTAAAAACTTGGCCACAGATGCTCG<br /> |rRNA<br /> |-<br /> |GATTTCTCGGGCCCCGTGCTACTTGGGTGTCTCTCAAACGAG<br /> |rRNA<br /> |-<br /> |GAATATTTAGGCTTAGCGGGTGGTCCCGCCAGATTCACACGGGATTTCTC<br /> |rRNA<br /> |-<br /> |CTCACGGTACTATCCGCTATCGGTCACCAGGGAATATTTAGGCTTAG<br /> |rRNA<br /> |-<br /> |GTTTCAGGTACTATTTCACTCCGCTCCCGCGGTACTTTTCACCATTC<br /> |rRNA<br /> |-<br /> |GATGGAGCAAGCTCCATCCCGACGCTCCCACGGCTTGTAGGCACACGGTTTCAGGTACT<br /> |rRNA<br /> |-<br /> |CACGCAGTCACGAGGATGGAGCAAGCTCCATCCCGACGCTC<br /> |rRNA<br /> |-<br /> |CTCGGGTTAACCTCGCAACACACCGCAAACTCGCAGGCTCATTCTTCAAAAGGCACGC<br /> |rRNA<br /> |-<br /> |CTACGGCTTCCCCACTCGGGTTAACCTCGCAACACACCGC<br /> |rRNA<br /> |-<br /> |GTCTTGAGCGTGCTACTAAAAACGCCCTATTCGGACTCGC<br /> |rRNA<br /> |-<br /> |CTCCCACGTCCTTCATCGGTTCCTGGTGCCAAGGCATCCAC<br /> |rRNA<br /> |-<br /> |CTTCAACCTGCCCATGGCTAGATCACTCCGCTTCGGGTCTTGAG<br /> |rRNA<br /> |-<br /> |GGTTCGGTCCTCCACGACCTCTTACAGCCGCTTCAACCTG<br /> |rRNA<br /> |-<br /> |CTAACCACAGGTCATCCCCCAGGTTTTCAACCCTGGTGGGTTCGGTCC<br /> |rRNA<br /> |-<br /> |AGAACCAGCTATCACGGAGTTTGATTGGCCTTTCACCCCTAACCACAGGTCATC<br /> |rRNA<br /> |-<br /> |CAAGAAACACACGACGCTGCACCTAAATGCATTTCGGGGAGAACCAGCTATCA<br /> |rRNA<br /> |-<br /> |TTTTAGCCTTGCGGCCGTACTCCCCAGGCGGGGCACTTAATGCGTTAGCTGCGGCAC<br /> |rRNA<br /> |-<br /> |CCGTCAATTCCTTTGAGTTTTAGCCTTGCGGCCGTACTCC<br /> |rRNA<br /> |-<br /> |AATAATCCGACCGTTCACAGCGTCCTCGCTGTTGTGTTACTTCAAAGGAA<br /> |rRNA<br /> |-<br /> |TTCTTCGCGTTGCGTCGAATTAAGCCACATGCTCCGCCGCTTGTGCGGGCCCCCGTCAA<br /> |rRNA<br /> |-<br /> |GTGTATGTCAAGCCTTGGTAAGGTTCTTCGCGTTGCGTCGAATTAAG<br /> |rRNA<br /> |-<br /> |GGGGGCACCATCTCTGATGCTTTCCGGTGTATGTCAAGCC<br /> |rRNA<br /> |-<br /> |TCACGACACGAGCTGACGACAGCCATGCACCACCTGTACACCGACCACAAGGGGGGCACC<br /> |rRNA<br /> |-<br /> |CTTAACCCAACATCTCACGACACGAGCTGACGACAGCCATG<br /> |rRNA<br /> |-<br /> |CAACACGGGACAAGGGTTGCGCTCGTTGCGGGACTTAACC<br /> |rRNA<br /> |-<br /> |GTCTCCCGTGAGTCCCCAACACCCCGAAGGGCTTGCTGGCAAC<br /> |rRNA<br /> |-<br /> |ACATAAGGGGCATGATGACTTGACGTCGTCCCCACCTTCCTCCGAGTTGACCCCG<br /> |rRNA<br /> |-<br /> |ATTGTACCGGCCATTGTAGCACGTGTGCAGCCCAAGACATAAGGGGCATGATGACTTGA<br /> |rRNA<br /> |-<br /> |TTTTGAGATTCGCTCCACCTTGCGGTATCGCAGCTCATTGTACCGGCCATTGTAGCACGT<br /> |rRNA<br /> |-<br /> |CAATCCGAACTGAGACCGGCTTTTTGAGATTCGCTCCACCTTGCGGTAT<br /> |rRNA<br /> |-<br /> |ATTACTAGCGACTCCGACTTCATGGGGTCGAGTTGCAGACCCCAATCCGAACTGAGA<br /> |rRNA<br /> |-<br /> |TATTCACCGCAGCAATGCTGATCTGCGATTACTAGCGACTCCGACTTCATG<br /> |rRNA<br /> |-<br /> |GTGTACAAGGCCCGGGAACGTATTCACCGCAGCAATGCTG<br /> |rRNA<br /> |-<br /> |GTAATCGGGTGACACCACGAGAGCGGAACAGTCGGAGGAATAA<br /> |rRNA<br /> |-<br /> |GTGTTACCGACTTTCGTGACGTGACGGGCGGTGTGTACAAG<br /> |rRNA<br /> |-<br /> |CACCTTCGACAGCTCCCTCCCACAAGGGGTTGGGCCACCGGCTTCGGGTGTTA<br /> |rRNA<br /> |-<br /> |CTACCTTGTTACGACTTCGTCCCAATCGCCAGTCCCACCTTCGACAG<br /> |rRNA<br /> |-<br /> |TCCTTAGAAAGGAGGTGATCCAGCCGCACCTTCCGGTACGGCTACCTTGTTACGA<br /> |rRNA<br /> |-<br /> |TGCAGTCGGCTATGTGCTCCTTAGAAAGGAGGTGATCCAG<br /> |rRNA<br /> |-<br /> |GATCAAACTCTCCGTGAATGTATACCCGTAATCGGGTGAC<br /> |rRNA<br /> |-<br /> |AAGTGGTTCATCGTTCGACTTGCATGTGTTAAGCACGCCGCCAGCGTTCGTCCTGA<br /> |rRNA<br /> |-<br /> |TATCAACATATCTGGCGTTGACTTTTGGCACGCTGTTGAGTTCTCAAGGAA<br /> |rRNA<br /> |-<br /> |TGTTACTCACCCGTTCGCCACTAATCCCCACCGAAGTGGTTCATCGTTCGACTT<br /> |rRNA<br /> |-<br /> |CTTGTCCCAGAGTGAAGGGCAGATTGCCCACGTGTTACTC<br /> |rRNA<br /> |-<br /> |GCAGATGCCTGCGAGGGTCAGTATCCGGTATTAGACCCCGTTTCCAGGGCTTGTCCCA<br /> |rRNA<br /> |-<br /> |ATCCTTCACCGCCGGAGCTTTCGAACCTCGCAGATGCCTGCGAGGGTCAGTATCC<br /> |rRNA<br /> |-<br /> |GATAGGCCGCGGGCTCATCCTTCACCGCCGGAGCTTTCGA<br /> |rRNA<br /> |-<br /> |GTCGCCTTGGTGAGCCATTACCTCACCAACAAGCTGATAG<br /> |rRNA<br /> |-<br /> |TAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGGCCGGTCGCCCTCTCAGGCCGGCTA<br /> |rRNA<br /> |-<br /> |ATTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTC<br /> |rRNA<br /> |-<br /> |CGTCGCTGCATCAGGCTTTCGCCCATTGTGCAATATTCCC<br /> |rRNA<br /> |-<br /> |CTTCTTCCCTGCTGAAAGAGGTTTACAACCCGAAGGCCGTCATC<br /> |rRNA<br /> |-<br /> |TTCAAAGGAACCTCAACCCGAACGGAGATCCGATCAGGTCGGGGTATCAACATAT<br /> |rRNA<br /> |-<br /> |GTAGTTAGCCGGCGCTTCTTCTGCAGGTACCGTCACTTTC<br /> |rRNA<br /> |-<br /> |ATAATTCCGGACAACGCTTGCGCCCTACGTATTACCGCGGCTGCTGGCACGTAGTTA<br /> |rRNA<br /> |-<br /> |CTTTCACAACCGACGTGACAAGCCGCCTACGAGCTCTTTACGCCCAATAATT<br /> |rRNA<br /> |-<br /> |TATCGACTGCAGACCCGGGGTTAAGCCCCGGGCTTTCACAACCGACGTGACAAG<br /> |rRNA<br /> |-<br /> |GATCTCCCCTACCGAACTCTAGCCTGCCCGTATCGACTGCAGAC<br /> |rRNA<br /> |-<br /> |CTGATATCTGCGCATTTCACCGCTACACCAGGAATTCCGATCTC<br /> |rRNA<br /> |-<br /> |CAGAGATCCGCCTTCGCCACCGGTGTTCCTCCTGATATCTG<br /> |rRNA<br /> |-<br /> |CACGCTTTCGCTCCTCAGCGTCAGTATCGGCCCAGAGATC<br /> |rRNA<br /> |-<br /> |CACCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTTC<br /> |rRNA<br /> |-<br /> |ACTTAATGCGTTAGCTGCGGCACGGACAACGTGGAATGTTGCCCACACCTAGTGCCCA<br /> |rRNA<br /> |-<br /> |GTTCGAAATGTAACCGGGCGTTTCCCCTACGCTATGACCACCGAAA<br /> |rRNA<br /> |-<br /> |CTTAGGATGGTTATAGTTACCACCGCCGTTTACTGGCGCTTAAGTTCTCA<br /> |rRNA<br /> |-<br /> |CCAAGCAAGCTTGATGTCCGACGCTCCCACGGCTTGTAGGCACACGGTTTCAGGTACTA<br /> |rRNA<br /> |-<br /> |CAGTCACGAGACACCAAGCAAGCTTGATGTCCGACGCTCC<br /> |rRNA<br /> |-<br /> |CTTGAGCGTGCTACTAAAAGCGCCCTATTCGGACTCGCTT<br /> |rRNA<br /> |-<br /> |CTACCTTGTTACGACTTCGTCCCAATCGCCAGTCCCACCTTCGACAGCTCCCT<br /> |rRNA<br /> |-<br /> |AAGGAGGTGATCCAGCCGCACCTTCCGGTACGGCTACCTTGTTACGACTTCGTC<br /> |rRNA<br /> |-<br /> |CCAGGATCAAACTCTCCGTGAATGTATACCCGTAATCGGG<br /> |rRNA<br /> |-<br /> |CTACCTTGTTACGACTTCGTCCCAATCGCCAGTCCCACCTTCG<br /> |rRNA<br /> |-<br /> |CTCTCACTTACCGGCATTCGGAGTTTGGCTAAGGTCAGTAAC<br /> |rRNA<br /> |-<br /> |CATTCGGACACCCCCGGATCAAAGCCTGGTTGACGACTCC<br /> |rRNA<br /> |-<br /> |GCTTATCCCCCACAGTCTCACTGCCGCGCTCTCACTTACC<br /> |rRNA<br /> |-<br /> |CTTAGTCGATGCTCTGGGCTGTTTCCCTCTCGACCATGGAGCTTATC<br /> |rRNA<br /> |-<br /> |CACATCCTTTCCCACTTAGCGTACGCTTAGGGGCCTTAGTCGATGCTCTG<br /> |rRNA<br /> |-<br /> |CTTCTAAGCCAACCTCCTGGTTGTCTGTGCGACTCCACATCCTTTC<br /> |rRNA<br /> |-<br /> |AATCACTAGACCAGTGAGCTATTACGCACTCTTTCAAGGGTGGCTGCTTCTAAGC<br /> |rRNA<br /> |-<br /> |CTACATTGTCGGCGCGGAATCACTAGACCAGTGAGCTATTA<br /> |rRNA<br /> |-<br /> |CGTATTGCTGCAATGACACGACTTCGGCGGTACGCTTGAG<br /> |rRNA<br /> |-<br /> |GCTCCCCTACCCATCACAGCCGGCGTTGGCCGTATTGCTG<br /> |rRNA<br /> |-<br /> |TTCTCACTCGTGAACCGTCCACAACTCGCTTCCGCGGCTGCTTCACCCGGCACACGACG<br /> |rRNA<br /> |-<br /> |GTTTGTATCGCTACTCATGCCTGCATTCTCACTCGTGAACCGTCCACAAC<br /> |rRNA<br /> |-<br /> |CTTAGTCAATCGGCGCAAACGTTTCTCACGTTTGTATCGCTACTCATG<br /> |rRNA<br /> |-<br /> |GACTTACCCTGGGCAGATCAGCTTGACCCAGGAACCCTTAGTCAATC<br /> |rRNA<br /> |-<br /> |TCCACACTGCCTATGTGTTCAGCAGCGGGTGACAGCCCATGACGACTGCCGGGTTT<br /> |rRNA<br /> |-<br /> |TTATCCATCGACTACGCCTGTCGGCCTCGCCTTAGGTCCCGACTTACCCTGGGCA<br /> |rRNA<br /> |-<br /> |GTACCGGAATATCAACCGGTTATCCATCGACTACGCCTGTC<br /> |rRNA<br /> |-<br /> |GGCCTTAGCATCACGATGCTCGATGTTTGACGCTTCACAG<br /> |rRNA<br /> |-<br /> |CTACTAACCGCTTGGTTCGGCGGCTCCACCACTCCCCTCAACTCCGAAGAGATCA<br /> |rRNA<br /> |-<br /> |GATGGACTACCTTCCTGCGTCACCCCATCACTCACCTACTAAC<br /> |rRNA<br /> |-<br /> |CCGGCCTACACCCTTACCCCGGGACAACCACCGCCCGGGATGGACTACC<br /> |rRNA<br /> |-<br /> |CAAGGCATCCACCGTGCGCCCTTAAAAACTTGGCCACAGA<br /> |rRNA<br /> |-<br /> |CAAGGTGTGCGGATTTACCTACACACCGGCCTACACCCTTAC<br /> |rRNA<br /> |-<br /> |CCACAATCGGCTCGGCATCAGGTCTCAGCCACAAGGTGTG<br /> |rRNA<br /> |-<br /> |CTAGAGGCTTTTCTCGACAGCATAGGATCATCCACTTCACCACAATC<br /> |rRNA<br /> |-<br /> |TTTAGGGTACGGGCCGCCATGAAACTCGCTAGAGGCTTTTCTC<br /> |rRNA<br /> |-<br /> |CTCGGTATTCTCTACCTGACCACCTGAGTCGGTTTAGGGTAC<br /> |rRNA<br /> |-<br /> |GGCATTTTGCCGAGTTCCTTAACCATAGTTCACCCGAACG<br /> |rRNA<br /> |-<br /> |CAGCTCAGAGTGCAAAACTCGTCACCAGGTGTGGCCCCCCTTCTCCCGAAGTTA<br /> |rRNA<br /> |-<br /> |TTGTTTTCTCTTCCTGCGGGTACTGAGATGTTTCACTTCCCCGCGTTCCCTCCACA<br /> |rRNA<br /> |-<br /> |TGTGTTTTTAGTAAACAGTCGCTTCTCGCTGGTCTCTGCGGCCACCCCCAGCTCAGA<br /> |rRNA<br /> |-<br /> |TATACATCGCCTTACGGCTTCGCACGGACCTGTGTTTTTAGTAAACAGTCGCTTCT<br /> |rRNA<br /> |-<br /> |GCAGGCGTCAGTCCGTATACATCGCCTTACGGCTTCGCAC<br /> |rRNA<br /> |-<br /> |GAAATGTGACTAACCGGTCCCCTTAACGTTCCAGCACCGGGCAGGC<br /> |rRNA<br /> |-<br /> |GTTTACTGGCGCTTAAGTTCTCAGCTTCGCCACACCGAAATGTGACTAAC<br /> |rRNA<br /> |-<br /> |GACAAGGAATTTCGCTACCTTAGGATGGTTATAGTTACCACCGCCGTTTA<br /> |rRNA<br /> |-<br /> |AAGTCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTA<br /> |rRNA<br /> |-<br /> |CTATGGTTGAGACAGTCGAGAAGTCGTTACGCCATTCGTG<br /> |rRNA<br /> |-<br /> |CTGCGCGAAACGAGCATCTTTACTCGTAGTGCAATTTCAC<br /> |rRNA<br /> |-<br /> |CAATATCAAACTGTAGTAAAGGTCCCGGGGTCTTTCCGTCCTGCTGCGCGAAA<br /> |rRNA<br /> |-<br /> |CAGCTATCCTACACAAGCCGAACCGAACACCAATATCAAACTGTAGTAAA<br /> |rRNA<br /> |-<br /> |CACACTGGCGTGCGAGCTTCAAAGTCTCCCAGCTATCCTACACAAG<br /> |rRNA<br /> |-<br /> |TTAGACATCCAGCACGACCAGACTGGTATTTCAACGACGACTCCACCCACACTGGCGTG<br /> |rRNA<br /> |-<br /> |GTTTCGCTCGCCACTACTCCCGGAATCACGGTTGTTTTCTCTTC<br /> |rRNA<br /> |-<br /> |GATCACGGACCCAGGTTAGACATCCAGCACGACCAGACTG<br /> |rRNA<br /> |-<br /> |CAGTTAAACTACCCATCAGACACTGTCCCTGATCCGGATCAC<br /> |rRNA<br /> |-<br /> |GAACCTTTGGGCGCCTCCGTTACTCTTTAGGAGGCAACCG<br /> |rRNA<br /> |-<br /> |CTTGTGCACTTACACTCAACACCTGATTGCCAACCAGGCTGAGGGAACCTTT<br /> |rRNA<br /> |-<br /> |GACCCGTCGGTCTCACAGTCAAGCTCCCTTGTGCACTTAC<br /> |rRNA<br /> |-<br /> |CTTCCACAAGCCACCGCCGGATCACTAGTCCCGACTTTCGTCCCTG<br /> |rRNA<br /> |-<br /> |GTACCTTTTATCCGTTGAGCGACGGCGCTTCCACAAGCCAC<br /> |rRNA<br /> |-<br /> |CAAACCATCCCGTCGATATGGACTCTTGGGGAAGATCAGCCTGTTATC<br /> |rRNA<br /> |-<br /> |CCAGGATGCGACGAGCCGACATCGAGGTGCCAAACCATCC<br /> |rRNA<br /> |-<br /> |GTCTCACACGTATACGGTTTGGCCTCATCCGGTTTCGCTC<br /> |rRNA<br /> |-<br /> |CTTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTCCAG<br /> |rRNA<br /> |-<br /> |GAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCGCTTTAATG<br /> |rRNA<br /> |-<br /> |CTTCTCAAGACTCCTACGCGCACAGCGGATAGGGACCGAAC<br /> |rRNA<br /> |-<br /> |CACACCAGAGGTTCGTCCGTCCCGGTCCTCTCGTACTAGG<br /> |rRNA<br /> |-<br /> |TTAGATGCTTTCAGCGGTTATCCCTCCCGAACGTAGCCAACCAGCCATGCCCTTGGCA<br /> |rRNA<br /> |-<br /> |CTCATCTCGAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATC<br /> |rRNA<br /> |-<br /> |CAGTCGTCTACTGGGAGCCTTAACCCCTCAAGGGGGTGGGAGTCCTCATCTCGAA<br /> |rRNA<br /> |-<br /> |CTTCCAGATCCGGCCTATCAACCCAGTCGTCTACTGGGAG<br /> |rRNA<br /> |-<br /> |CAGACCGTAAAAGAGAGATCCCACAACCCCGCACACGCAACCCCTGCCGGGTCTCA<br /> |rRNA<br /> |-<br /> |TACATCAACGGTTTCTGACTCGTCTCACGGCCGGCAGACCGTAAAAGAGAGAT<br /> |rRNA<br /> |-<br /> |CTTTCGCATGTCCTTCGCCTACATCAACGGTTTCTGACTC<br /> |rRNA<br /> |-<br /> |ACTACGGGGGTCTTACCCTCTACGCCGGACCTTTCGCATG<br /> |rRNA<br /> |-<br /> |CTACTTGGGTGTCTCTCAAACGAGCCGTTGACGTTTCGACTAC<br /> |rRNA<br /> |-<br /> |GCCAGATTCACACGGGATTTCTCGGGCCCCGTGCTACTTG<br /> |rRNA<br /> |-<br /> |TATCCGCTATCGGTCACCAGGGAATATTTAGGCTTAGCGGGTGGTCCCGCCAGATT<br /> |rRNA<br /> |-<br /> |GTACTTTTCACCATTCCCTCACGGTACTATCCGCTATCGGTCAC<br /> |rRNA<br /> |-<br /> |CTTGTAGGCACACGGTTTCAGGTACTATTTCACTCCGCTCCC<br /> |rRNA<br /> |-<br /> |GGGCCTATCGTGGCCTCCCACGTCCTTCATCGGTTCCTGG<br /> |rRNA<br /> |-<br /> |CACGCAGTCACGAGGATGGAGCAAGCTCCATCCCGACGCTCCCACGGCTTGTAGGCA<br /> |rRNA<br /> |-<br /> |CATTCTTCAAAAGGCACGCAGTCACGAGGATGGAGCAAGC<br /> |rRNA<br /> |-<br /> |TACGGCTTCCCCACTCGGGTTAACCTCGCAACACACCGCAAACTCGCAGGCTCAT<br /> |rRNA<br /> |-<br /> |TCGGACTCGCTTTCGCTACGGCTTCCCCACTCGGGTTAAC<br /> |rRNA<br /> |-<br /> |ATCACTCCGCTTCGGGTCTTGAGCGTGCTACTAAAAACGC<br /> |rRNA<br /> |-<br /> |CTCTTACAGCCGCTTCAACCTGCCCATGGCTAGATCACTC<br /> |rRNA<br /> |-<br /> |GTTTTCAACCCTGGTGGGTTCGGTCCTCCACGACCTCTTAC<br /> |rRNA<br /> |-<br /> |GTTTGATTGGCCTTTCACCCCTAACCACAGGTCATCCCCCAGGTTTTCAACC<br /> |rRNA<br /> |-<br /> |GGAGAACCAGCTATCACGGAGTTTGATTGGCCTTTCACCC<br /> |rRNA<br /> |-<br /> |TATCCAGTGCTCTACCTCCGGCAAGAAACACACGACGCTGCACCTAAATGCATTT<br /> |rRNA<br /> |-<br /> |GAGTTTGGCTAAGGTCAGTAACCCGGTAGGGCCCATCGCCTATCCAGTGCTCTA<br /> |rRNA<br /> |-<br /> |CTGTAAGGCTTAGCTTCCGGGTTCGAAATGTAACCGGGCGTTTCCCCTAC<br /> |rRNA<br /> |-<br /> |GTTCGGCGGCGTCCTACTCTCCCACAGGGTCCCCCCTGCAGTACCATCGGCGCTGTA<br /> |rRNA<br /> |-<br /> |GTATTAGACCCCGTTTCCAGGGCTTGTCCCAGAGTGAAGGGCAGATT<br /> |rRNA<br /> |-<br /> |GAGCTTTCGAACCTCGCAGATGCCTGCGAGGGTCAGTATCCGGTATTAGACCCCGTT<br /> |rRNA<br /> |-<br /> |CATCCTTCACCGCCGGAGCTTTCGAACCTCGCAGATGCCT<br /> |rRNA<br /> |-<br /> |CACCGAAGTGGTTCATCGTTCGACTTGCATGTGTTAAGCAC<br /> |rRNA<br /> |-<br /> |TTCGACTTGCATGTGTTAAGCACGCCGCCAGCGTTCGTCCTGAGCCAGGATCAAACTCT<br /> |rRNA<br /> |-<br /> |CAGATTGCCCACGTGTTACTCACCCGTTCGCCACTAATCC<br /> |rRNA<br /> |-<br /> |GCGGATTTACCTACCTGACGTCCTACACCCTTACCCCGGGACAACCACCGC<br /> |rRNA<br /> |-<br /> |CATCAGGTCTCAGACTCATGTCAGGCGGATTTACCTACCTGAC<br /> |rRNA<br /> |-<br /> |CTATTAGTACCGGTCACCTCCACACCTCACGGTGCTTCCAGATCCGGCCTATCAACC<br /> |rRNA<br /> |-<br /> |GGACAAGCCCTCGGCCTATTAGTACCGGTCACCTCCACAC<br /> |rRNA<br /> |-<br /> |CCGGGTTAACCTCGCAACACACCGCAAACTCGCAGGCTCATTCTTCAAAAGGCACGC<br /> |rRNA<br /> |-<br /> |GGTCTTGAGCGTGCTACTAAAACGCCCTATTCGGACTCGC<br /> |rRNA<br /> |-<br /> |TTCGGCGGCGTCCTACTCTCCCACAGGGTCCCCCCTGCAGTACCATCGGCGCTGTAA<br /> |rRNA<br /> |-<br /> |CCGAAGCCTCCGGCGGTCTGCTTTCTGTGGCACTGTCCCG<br /> |SCOs01<br /> |-<br /> |CTGGGCACTGGTGGTCTCTTACACCACCGTTTCACCCTTAC<br /> |SCOs01<br /> |-<br /> |TTCCTTCTTGACCTTGCTCCGGGTGGGGTTTACCTAGCTGCCCAGGTCGCCCTG<br /> |SCOs01<br /> |-<br /> |CTCGAACGTCCGCGCAGAAGCACCGGGGTGCTTCCTTCTTGACCTTGCTC<br /> |SCOs01<br /> |-<br /> |CTCCAGCGGTCTACCCGCGGACTCGGGCGGGCAGCCCTCGAACGTCCGCGCAGAAG<br /> |SCOs01<br /> |-<br /> |CATCCATCTAGGACCGGCGTTGCCACCGGCCTCCAGCGGTCTAC<br /> |SCOs01<br /> |-<br /> |CTGTGGAGCCCGGACGTTCCTCGGGAAGTCCCGTGAGGGACTC<br /> |SCOs01<br /> |-<br /> |GTTCTGTTCCGACGGGGCCTCGCGGTCCCGTCGGCGACGGCCATCCAT<br /> |SCOs01<br /> |-<br /> |CAGACGAGTCGGGCTGTACGCCGGGTTCTGTTCCGACGGG<br /> |SCOs01<br /> |-<br /> |GTCTGCTTTCTGTGGCACTGTCCCGCGGGTCACCCCGGGTGGCCGTTAGCCATCAC<br /> |SCOs01<br /> |-<br /> |CTTAGTAGGAGGCTCGCTTCGCTACCTGAGATCAGGCAGCGAGGGCGAAGG<br /> |SCOs02<br /> |-<br /> |GAACAGCCCCGGGCTGACACTCCCTTAGTAGGAGGCTCGCTTC<br /> |SCOs02<br /> |-<br /> |GATCAAGGTTTAGTCCCCTAGCTGATGCCAGGATCCGGGTC<br /> |SCOs02<br /> |-<br /> |CAGTCACTGTTTGGTTTCCCTCTTCACCCCGTGACCGGGATCAA<br /> |SCOs02<br /> |-<br /> |GAGATCACGCGGACAAGTCTCCGACGGGCCCAGTCACTGT<br /> |SCOs02<br /> |-<br /> |CTTCCCCTGCTTCGACAGCCGCTGTCGAAACCGATCATCC<br /> |SCOs02<br /> |-<br /> |AATCAGGGCTTCTCCGTGTGCAGTCCGCTTCGATTTTCTCGGCCCCGGAGATCACG<br /> |SCOs02<br /> |-<br /> |GAATCGAACCCGCGTCCAACGGTGCGGAATCAGGGCTTCTC<br /> |SCOs02<br /> |-<br /> |TTTTTCGGCCTGTGGTTTACGAGATCATGGCCGCTTCCTC<br /> |SCOs02<br /> |-<br /> |CTGCTGGGAGGAATCGCGCTTGGTGTTGGCGATTATTTTT<br /> |SCOs02<br /> |-<br /> |CACGCACCCGCCAGAGCCGACCTACCCTTGCTGCCTTCCG<br /> |SCOs03<br /> |}</div> RTEvans https://actinobase.org/index.php?title=RRNA_depletion_with_Streptomyces_coelicolor_A3(2)_specific_probes&diff=2196 RRNA depletion with Streptomyces coelicolor A3(2) specific probes 2023-11-01T16:50:01Z <p>RTEvans: /* Description */</p> <hr /> <div>==Organisms==<br /> <br /> This protocol has been tested with &lt;em&gt;[[Streptomyces coelicolor]]&lt;/em&gt; A3(2) MT1110<br /> <br /> ==Description==<br /> <br /> rRNA depletion using commercial kits targeting generic bacterial sequences is not 100% efficient in the High GC organism &lt;em&gt;S. coelicolor&lt;/em&gt;. <br /> <br /> Depletion using the NEBNext RNA Depletion Core Reagent Set with RNA Sample Purification Beads (https://www.neb.com/en-gb/products/e7870-nebnext-rna-depletion-core-reagent-set-with-rna-sample-purification-beads#Product%20Information) and custom DNA probes designed for &lt;em&gt;S. coelicolor&lt;/em&gt; is more effective than the QIAseq FastSelect 5S/16S/23S kit (Qiagen #335927); NEBNext rRNA Depletion Kit (Bacteria) (NEB #E7850); and Illumina Ribo-zero rRNA Removal Kit (Bacteria) (Illumina #MRZMB12424, discontinued). The Illumina kit tested was subsequently replaced by the Illumina Ribo-Zero Plus rRNA Depletion Kit (Illumina #20040526). <br /> <br /> In addition, three abundant non-mRNA RNAs can also be depleted using the custom probes (Table 1).<br /> <br /> '''Table 1.''' Abundant non-mRNA RNAs in &lt;em&gt;S. coelicolor&lt;/em&gt;.<br /> <br /> [[File: rRNA_depletion_table_1.png]]<br /> <br /> ==Materials==<br /> <br /> Single stranded DNA probes (Table 2) at 2µM concentration in IDTE pH 7.5 (10mM Tris-HCl / 0.1mM EDTA). Integrated DNA Technologies can produce these from their 25nmole DNA oligo pool product. Probes are listed in Table 2 in the Notes section below.<br /> <br /> NEBNext RNA Depletion Core Reagent Set with RNA Sample Purification Beads (https://www.neb.com/en-gb/products/e7870-nebnext-rna-depletion-core-reagent-set-with-rna-sample-purification-beads#Product%20Information).<br /> <br /> ==Protocols==<br /> <br /> As per manufacturer’s instructions.<br /> <br /> ==Notes==<br /> <br /> The following histograms illustrate effectiveness of rRNA depletion using various methods. Histograms show percentage of bases aligned to NCBI reference sequence NC_003888.3 using Hisat2 program classified as follows - Coding = RNA aligned to coding sequences; Transcribed not translated = RNA aligned to rRNA, tRNA and sRNA; Intergenic = RNA aligned to 5' or 3' UTRs or between annotated sequences.<br /> <br /> '''QIAseq FastSelect 5S/16S/23S kit'''<br /> <br /> [[File: PanelA.png]] <br /> <br /> '''NEBNext rRNA Depletion Kit (Bacteria)'''<br /> <br /> [[File: PanelB.png]]<br /> <br /> '''Illumina Ribo-zero rRNA Removal Kit (Bacteria)'''<br /> <br /> [[File: PanelC.png]] <br /> <br /> '''rRNA depletion with &lt;em&gt;S. coelicolor&lt;/em&gt; specific probes<br /> <br /> [[File: PanelD.png]]<br /> <br /> <br /> '''Table 2''' List of probes<br /> <br /> {| class=&quot;wikitable&quot;<br /> !Sequence<br /> !Target<br /> |-<br /> |CAAGTCCTTGCGCGACGCTCCCACGGCTTGTAGGCACACGGTTTCAGGTACTATTT<br /> |rRNA<br /> |-<br /> |CAGTCACGACGCAAGGACAAGTCCTTGCGCGACGCTCCCAC<br /> |rRNA<br /> |-<br /> |ACCTCGCAACACACCGCAAACTCGCAGGCTCATTCTTCAAAAGGCACGCAGTCACGACG<br /> |rRNA<br /> |-<br /> |CTACGGCTTCCCCACCCGGGTTAACCTCGCAACACACCGC<br /> |rRNA<br /> |-<br /> |TTAACCCAACATCTCACGACACGAGCTGACGACAGCCATGCACCACCTGTACACCGA<br /> |rRNA<br /> |-<br /> |CGCTCGTTGCGGGACTTAACCCAACATCTCACGACACGAG<br /> |rRNA<br /> |-<br /> |CAACACCCCGAAGGGCTTGCTGGCAACACGGGACAAGGGTTG<br /> |rRNA<br /> |-<br /> |CACCTTCCTCCGAGTTGACCCCGGCGGTCTCCCGTGAGTC<br /> |rRNA<br /> |-<br /> |GACATAAGGGGCATGATGACTTGACGTCGTCCCCACCTTC<br /> |rRNA<br /> |-<br /> |GTATCGCAGCTCATTGTACCGGCCATTGTAGCACGTGTGCAGCCCAAGACATAA<br /> |rRNA<br /> |-<br /> |TTTTGAGATTCGCTCCACCTTGCGGTATCGCAGCTCATTGTAC<br /> |rRNA<br /> |-<br /> |CAGACCCCAATCCGAACTGAGACCGGCTTTTTGAGATTCG<br /> |rRNA<br /> |-<br /> |AATGCTGATCTGCGATTACTAGCGACTCCGACTTCATGGGGTCGAGTTGCAGAC<br /> |rRNA<br /> |-<br /> |GAACGTATTCACCGCAGCAATGCTGATCTGCGATTACTAG<br /> |rRNA<br /> |-<br /> |TACCGACTTTCGTGACGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTC<br /> |rRNA<br /> |-<br /> |GCCACCGGCTTCGGGTGTTACCGACTTTCGTGACGTGACG<br /> |rRNA<br /> |-<br /> |TTCGTCCCAATCGCCAGTCCCACCTTCGACAGCTCCCTCCCACAAGGGGTTGGG<br /> |rRNA<br /> |-<br /> |CGCACCTTCCGGTACGGCTACCTTGTTACGACTTCGTCCCAATCGCCAGTCCCA<br /> |rRNA<br /> |-<br /> |TTCGACCCTCGCAGATGCCTGCGAGGGTCAGTATCCGGTATTAGACCCCGTTTCCAG<br /> |rRNA<br /> |-<br /> |GAAAGGAGGTGATCCAGCCGCACCTTCCGGTACGGCTACC<br /> |rRNA<br /> |-<br /> |TTCACCGCCGGAGCTTTCGACCCTCGCAGATGCCTGCGAG<br /> |rRNA<br /> |-<br /> |CATTACCTCACCAACAAGCTGATAGGCCGCGGGCTCATCCTTC<br /> |rRNA<br /> |-<br /> |CTCTCAGGCCGGCTACCCGTCGTCGCCTTGGTGAGCCATTAC<br /> |rRNA<br /> |-<br /> |GTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGGCCGGTCG<br /> |rRNA<br /> |-<br /> |TGCATCAGGCTTTCGCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCT<br /> |rRNA<br /> |-<br /> |TGCTGAAAGAGGTTTACAACCCGAAGGCCGTCATCCCTCACGCGGCGTCGCTGCATCA<br /> |rRNA<br /> |-<br /> |TTCTTCTGCAGGTACCGTCACTTTCGCTTCTTCCCTGCTGAAAGAGGTTTACAA<br /> |rRNA<br /> |-<br /> |CTACGTATTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTCTG<br /> |rRNA<br /> |-<br /> |CTCTTTACGCCCAATAATTCCGGACAACGCTTGCGCCCTACGTATTAC<br /> |rRNA<br /> |-<br /> |GGGCTTTCACAACCGACGTGACAAGCCGCCTACGAGCTCT<br /> |rRNA<br /> |-<br /> |CTACCGAACTCTAGCCTGCCCGTATCGACTGCAGACCCGGGGTTAAGCCCCG<br /> |rRNA<br /> |-<br /> |ATATCTGCGCATTTCACCGCTACACCAGGAATTCCGATCTCCCCTACCGAACTCTAG<br /> |rRNA<br /> |-<br /> |CGGTGTTCCTCCTGATATCTGCGCATTTCACCGCTACACC<br /> |rRNA<br /> |-<br /> |ACGCTTTCGCTCCTCAGCGTCAGTATCGGCCCAGAGATCCGCCTTCGCCACCGGTGT<br /> |rRNA<br /> |-<br /> |GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCG<br /> |rRNA<br /> |-<br /> |ACAACGTGGAATGTTGCCCACACCTAGTGCCCACCGTTTACGGCGTGGACTACCA<br /> |rRNA<br /> |-<br /> |CTTAATGCGTTAGCTGCGGCACGGACAACGTGGAATGTTG<br /> |rRNA<br /> |-<br /> |TCAATTCCTTTGAGTTTTAGCCTTGCGGCCGTACTCCCCAGGCGGGGCACTTAA<br /> |rRNA<br /> |-<br /> |ATTAAGCCACATGCTCCGCCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTTA<br /> |rRNA<br /> |-<br /> |TCAAGCCTTGGTAAGGTTCTTCGCGTTGCGTCGAATTAAGCCACATGCTCCGCCGCTT<br /> |rRNA<br /> |-<br /> |CATCTCTGATGCTTTCCGGTGTATGTCAAGCCTTGGTAAGGTTCTTC<br /> |rRNA<br /> |-<br /> |ACAGCCATGCACCACCTGTACACCGACCACAAGGGGGGCACCATCTCTGATGCTTTCC<br /> |rRNA<br /> |-<br /> |TGTAACCGGGCGTTTCCCCTACGCTATGACCACCGAAACC<br /> |rRNA<br /> |-<br /> |CAGTACCATCGGCGCTGTAAGGCTTAGCTTCCGGGTTCGAAATGTAAC<br /> |rRNA<br /> |-<br /> |GTTCGGCGGCGTCCTACTCTCCCACAGGGTCCCCCCTGCA<br /> |rRNA<br /> |-<br /> |CACTCCCCTCAACTCCGAAGAGATCAGGGCGGCTTCACGG<br /> |rRNA<br /> |-<br /> |CCATCACTCACCTACTAACCGCTTGGTTCGGCGGCTCCAC<br /> |rRNA<br /> |-<br /> |GTCCTTCATCGGTTCCTGGTGCCAAGGCATCCACCGTGCGCCCTTAAAAACTT<br /> |rRNA<br /> |-<br /> |CTTGTAGGCACACGGTTTCAGGTACTATTTCACTCCGCTCCCG<br /> |rRNA<br /> |-<br /> |CACGCAGTCACGAGACACCAAGCAAGCTTGATGTCCGACGCTCCCACGGCTTGTAGGCAC<br /> |rRNA<br /> |-<br /> |TCTTCAAAAGGCACGCAGTCACGAGACACCAAGCAAGCTT<br /> |rRNA<br /> |-<br /> |CACCCGGGTTAACCTCGCAACACACTGCAAACTCGCAGGC<br /> |rRNA<br /> |-<br /> |GCGTGCTACTAAAACGCCCTATTCGGACTCGCTTTCGCTACGGCTTCC<br /> |rRNA<br /> |-<br /> |GATCACTCCGCTTCGGGTCTTGAGCGTGCTACTAAAACGC<br /> |rRNA<br /> |-<br /> |TCGCAGATGCCTGCAAGGGTCAGTATCCGGTATTAGACCCCGTTT<br /> |rRNA<br /> |-<br /> |TCATCCTTCACCGCCGCAGCTTTCGAACCTCGCAGATGCC<br /> |rRNA<br /> |-<br /> |ATGTAACCGGGCGTTTCCCCTACGCTATGACCACCGAAAC<br /> |rRNA<br /> |-<br /> |CGTCCTACTCTCCCACAGGGTCCCCCCTGCAGTACCATCG<br /> |rRNA<br /> |-<br /> |CATCGCCTATCCAGTGCTCTACCTCCGGCAAGAAACACAC<br /> |rRNA<br /> |-<br /> |CATTCGGAGTTTGGCTAAGGTCAGTAACCCGGTAGGGCCCATCG<br /> |rRNA<br /> |-<br /> |CAGTCTCACTGCCGCGCTCTCACTTACCGGCATTCGGAGTTTG<br /> |rRNA<br /> |-<br /> |ATGCTCTGGGCTGTTTCCCTCTCGACCATGGAGCTTATCCCCCACAGTCTCACTG<br /> |rRNA<br /> |-<br /> |CTTAGGGGCCTTAGTCGATGCTCTGGGCTGTTTCCCTCTCG<br /> |rRNA<br /> |-<br /> |GTTGTCTGTGCGACTCCACATCCTTTCCCACTTAGCGTACGCTTAG<br /> |rRNA<br /> |-<br /> |TATTACGCACTCTTTCAAGGGTGGCTGCTTCTAAGCCAACCTCCTGGTTGTCTGT<br /> |rRNA<br /> |-<br /> |GATCAAAGCCTGGTTGACGACTCCCCGGGGCCTATCGTGG<br /> |rRNA<br /> |-<br /> |CGGAATCACTAGACCAGTGAGCTATTACGCACTCTTTCAAGG<br /> |rRNA<br /> |-<br /> |TATTGCTGCAATGACACGACTTCGGCGGTACGCTTGAGCCCCGCTACATTGTCG<br /> |rRNA<br /> |-<br /> |CATCACAGCCGGCGTTGGCCGTATTGCTGCAATGACACGACTTC<br /> |rRNA<br /> |-<br /> |TCCACAACTCGCTTCCGCGGCTGCTTCACCCGGCACACGACGCTCCCCTACCCATCACA<br /> |rRNA<br /> |-<br /> |TACTCATGCCTGCATTCTCACTCGTGAACCGTCCACAACTCGCTTCCGCGGCTGCTT<br /> |rRNA<br /> |-<br /> |CAAACGTTTCTCACGTTTGTATCGCTACTCATGCCTGCATTCTCACTC<br /> |rRNA<br /> |-<br /> |CAGGAACCCTTAGTCAATCGGCGCAAACGTTTCTCACGTTT<br /> |rRNA<br /> |-<br /> |CTTAGGTCCCGACTTACCCTGGGCAGATCAGCTTGACCCAG<br /> |rRNA<br /> |-<br /> |GAATATCAACCGGTTATCCATCGACTACGCCTGTCGGCCTCGCCTTAGGTC<br /> |rRNA<br /> |-<br /> |TTAGCATCACGATGCTCGATGTTTGACGCTTCACAGCGGGTACCGGAATATCAA<br /> |rRNA<br /> |-<br /> |GAAGAGATCAGGGCGGCTTCACGGCCTTAGCATCACGATG<br /> |rRNA<br /> |-<br /> |CATCACTCACCTACTAACCGCTTGGTTCGGCGGCTCCACC<br /> |rRNA<br /> |-<br /> |TGTGTTCAGCAGCGGGTGACAGCCCATGACGACTGCCGGGTTTCCCCATTCGGACACCC<br /> |rRNA<br /> |-<br /> |GACAACCACCGCCCGGGATGGACTACCTTCCTGCGTCACCCCAT<br /> |rRNA<br /> |-<br /> |CAGATGCTCGCGTCCACTGTGCAGTTCTCAAACAACGACC<br /> |rRNA<br /> |-<br /> |TGCGGATTTACCTACACACCGGCCTACACCCTTACCCCGGGACAACCACCG<br /> |rRNA<br /> |-<br /> |CATCAGGTCTCAGCCACAAGGTGTGCGGATTTACCTACAC<br /> |rRNA<br /> |-<br /> |TTTTCTCGACAGCATAGGATCATCCACTTCACCACAATCGGCTCGGCATCAGGTCT<br /> |rRNA<br /> |-<br /> |CCATGAAACTCGCTAGAGGCTTTTCTCGACAGCATAGGATCATC<br /> |rRNA<br /> |-<br /> |TATTCTCTACCTGACCACCTGAGTCGGTTTAGGGTACGGGCCGCCATGAAACT<br /> |rRNA<br /> |-<br /> |GAGTTCCTTAACCATAGTTCACCCGAACGCCTCGGTATTCTCTACCTGAC<br /> |rRNA<br /> |-<br /> |CTTCTCCCGAAGTTACGGGGGCATTTTGCCGAGTTCCTTAACCA<br /> |rRNA<br /> |-<br /> |CACCCCCAGCTCAGAGTGCAAAACTCGTCACCAGGTGTGG<br /> |rRNA<br /> |-<br /> |GTGTTTTTAGTAAACAGTCGCTTCTCGCTGGTCTCTGCGG<br /> |rRNA<br /> |-<br /> |GTTCCCTCCACACTGCCTATGTGTTCAGCAGCGGGTGACAG<br /> |rRNA<br /> |-<br /> |GTATACATCGCCTTACGGCTTCGCACGGACCTGTGTTTTTA<br /> |rRNA<br /> |-<br /> |CTTAACGTTCCAGCACCGGGCAGGCGTCAGTCCGTATACATC<br /> |rRNA<br /> |-<br /> |TAAGTTCTCAGCTTCGCCACACCGAAATGTGACTAACCGGTCCCCTTAACGTT<br /> |rRNA<br /> |-<br /> |TTAGGATGGTTATAGTTACCACCGCCGTTTACTGGCGCTTAAGTTCTCAG<br /> |rRNA<br /> |-<br /> |GTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGATGGTTATAGT<br /> |rRNA<br /> |-<br /> |TATGGTTGAGACAGTCGAGAAGTCGTTACGCCATTCGTGCAGGTCGGAACTTACC<br /> |rRNA<br /> |-<br /> |GAAACGAGCATCTTTACTCGTAGTGCAATTTCACCGGGCCTATGGTTGAG<br /> |rRNA<br /> |-<br /> |GGTCTTTCCGTCCTGCTGCGCGAAACGAGCATCTTTACTC<br /> |rRNA<br /> |-<br /> |GAACCGAACACCAATATCAAACTGTAGTAAAGGTCCCGGGGTCTTTC<br /> |rRNA<br /> |-<br /> |CTTCAAAGTCTCCCAGCTATCCTACACAAGCCGAACCGAACACCAATA<br /> |rRNA<br /> |-<br /> |AGACTGGTATTTCAACGACGACTCCACCCACACTGGCGTGCGAGCTTCAAAGTCT<br /> |rRNA<br /> |-<br /> |GTTAGACATCCAGCACGACCAGACTGGTATTTCAACGACGACTC<br /> |rRNA<br /> |-<br /> |TTAAACTACCCATCAGACACTGTCCCTGATCCGGATCACGGACCCAGGTTAGACAT<br /> |rRNA<br /> |-<br /> |GAATCACGGTTGTTTTCTCTTCCTGCGGGTACTGAGATGTTTCACTTC<br /> |rRNA<br /> |-<br /> |GTTACTCTTTAGGAGGCAACCGCCCCAGTTAAACTACCCATCAGACACTG<br /> |rRNA<br /> |-<br /> |ACTCAACACCTGATTGCCAACCAGGCTGAGGGAACCTTTGGGCGCCTCCGTTACT<br /> |rRNA<br /> |-<br /> |GTCTCACAGTCAAGCTCCCTTGTGCACTTACACTCAACACCTGATTG<br /> |rRNA<br /> |-<br /> |GACTTTCGTCCCTGCTCGACCCGTCGGTCTCACAGTCAAG<br /> |rRNA<br /> |-<br /> |TACCTTTTATCCGTTGAGCGACGGCGCTTCCACAAGCCACCGCCGGATCACTAGTC<br /> |rRNA<br /> |-<br /> |GTCGATATGGACTCTTGGGGAAGATCAGCCTGTTATCCCCGGGGTACCTTTTAT<br /> |rRNA<br /> |-<br /> |GACATCGAGGTGCCAAACCATCCCGTCGATATGGACTCTTG<br /> |rRNA<br /> |-<br /> |TTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTCCAGCCCCAGGATGCGACGA<br /> |rRNA<br /> |-<br /> |TCACGACGTTCTAAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACC<br /> |rRNA<br /> |-<br /> |GATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTC<br /> |rRNA<br /> |-<br /> |GTCCTCTCGTACTAGGGACAGCCCTTCTCAAGACTCCTAC<br /> |rRNA<br /> |-<br /> |TCTCACACGTATACGGTTTGGCCTCATCCGGTTTCGCTCGCCACTACTCCCGGAAT<br /> |rRNA<br /> |-<br /> |CATGCCCTTGGCAGGACAACTGGCACACCAGAGGTTCGTC<br /> |rRNA<br /> |-<br /> |CTTAGATGCTTTCAGCGGTTATCCCTCCCGAACGTAGCCAAC<br /> |rRNA<br /> |-<br /> |GTGGGAGTCCTCATCTCGAAGCAGGCTTCCCGCTTAGATG<br /> |rRNA<br /> |-<br /> |CTATCAACCCAGTCGTCTACTGGGAGCCTTAACCCCTCAAGG<br /> |rRNA<br /> |-<br /> |GCCTATTAGTACCGGTCACCTCCACACCTCACGGTGCTTCCAGATCCGGCCTATCAAC<br /> |rRNA<br /> |-<br /> |AGGACAAGCCCTCGGCCTATTAGTACCGGTCACCTCCACA<br /> |rRNA<br /> |-<br /> |CACACGCAACCCCTGCCGGGTCTCACACGTATACGGTTTG<br /> |rRNA<br /> |-<br /> |TTTCTGACTCGTCTCACGGCCGGCAGACCGTAAAAGAGAGATCCCACAACCCCGCACA<br /> |rRNA<br /> |-<br /> |TTTCGCATGTCCTTCGCCTACATCAACGGTTTCTGACTCGTCTCACGGCCGGCAGA<br /> |rRNA<br /> |-<br /> |CTTACCCTCTACGCCGGACCTTTCGCATGTCCTTCGCCTACATC<br /> |rRNA<br /> |-<br /> |GTGTCTCTCAAACGAGCCGTTGACGTTTCGACTACGGGGGTCTTACCCTCTA<br /> |rRNA<br /> |-<br /> |ATCCACCGTGCGCCCTTAAAAACTTGGCCACAGATGCTCG<br /> |rRNA<br /> |-<br /> |GATTTCTCGGGCCCCGTGCTACTTGGGTGTCTCTCAAACGAG<br /> |rRNA<br /> |-<br /> |GAATATTTAGGCTTAGCGGGTGGTCCCGCCAGATTCACACGGGATTTCTC<br /> |rRNA<br /> |-<br /> |CTCACGGTACTATCCGCTATCGGTCACCAGGGAATATTTAGGCTTAG<br /> |rRNA<br /> |-<br /> |GTTTCAGGTACTATTTCACTCCGCTCCCGCGGTACTTTTCACCATTC<br /> |rRNA<br /> |-<br /> |GATGGAGCAAGCTCCATCCCGACGCTCCCACGGCTTGTAGGCACACGGTTTCAGGTACT<br /> |rRNA<br /> |-<br /> |CACGCAGTCACGAGGATGGAGCAAGCTCCATCCCGACGCTC<br /> |rRNA<br /> |-<br /> |CTCGGGTTAACCTCGCAACACACCGCAAACTCGCAGGCTCATTCTTCAAAAGGCACGC<br /> |rRNA<br /> |-<br /> |CTACGGCTTCCCCACTCGGGTTAACCTCGCAACACACCGC<br /> |rRNA<br /> |-<br /> |GTCTTGAGCGTGCTACTAAAAACGCCCTATTCGGACTCGC<br /> |rRNA<br /> |-<br /> |CTCCCACGTCCTTCATCGGTTCCTGGTGCCAAGGCATCCAC<br /> |rRNA<br /> |-<br /> |CTTCAACCTGCCCATGGCTAGATCACTCCGCTTCGGGTCTTGAG<br /> |rRNA<br /> |-<br /> |GGTTCGGTCCTCCACGACCTCTTACAGCCGCTTCAACCTG<br /> |rRNA<br /> |-<br /> |CTAACCACAGGTCATCCCCCAGGTTTTCAACCCTGGTGGGTTCGGTCC<br /> |rRNA<br /> |-<br /> |AGAACCAGCTATCACGGAGTTTGATTGGCCTTTCACCCCTAACCACAGGTCATC<br /> |rRNA<br /> |-<br /> |CAAGAAACACACGACGCTGCACCTAAATGCATTTCGGGGAGAACCAGCTATCA<br /> |rRNA<br /> |-<br /> |TTTTAGCCTTGCGGCCGTACTCCCCAGGCGGGGCACTTAATGCGTTAGCTGCGGCAC<br /> |rRNA<br /> |-<br /> |CCGTCAATTCCTTTGAGTTTTAGCCTTGCGGCCGTACTCC<br /> |rRNA<br /> |-<br /> |AATAATCCGACCGTTCACAGCGTCCTCGCTGTTGTGTTACTTCAAAGGAA<br /> |rRNA<br /> |-<br /> |TTCTTCGCGTTGCGTCGAATTAAGCCACATGCTCCGCCGCTTGTGCGGGCCCCCGTCAA<br /> |rRNA<br /> |-<br /> |GTGTATGTCAAGCCTTGGTAAGGTTCTTCGCGTTGCGTCGAATTAAG<br /> |rRNA<br /> |-<br /> |GGGGGCACCATCTCTGATGCTTTCCGGTGTATGTCAAGCC<br /> |rRNA<br /> |-<br /> |TCACGACACGAGCTGACGACAGCCATGCACCACCTGTACACCGACCACAAGGGGGGCACC<br /> |rRNA<br /> |-<br /> |CTTAACCCAACATCTCACGACACGAGCTGACGACAGCCATG<br /> |rRNA<br /> |-<br /> |CAACACGGGACAAGGGTTGCGCTCGTTGCGGGACTTAACC<br /> |rRNA<br /> |-<br /> |GTCTCCCGTGAGTCCCCAACACCCCGAAGGGCTTGCTGGCAAC<br /> |rRNA<br /> |-<br /> |ACATAAGGGGCATGATGACTTGACGTCGTCCCCACCTTCCTCCGAGTTGACCCCG<br /> |rRNA<br /> |-<br /> |ATTGTACCGGCCATTGTAGCACGTGTGCAGCCCAAGACATAAGGGGCATGATGACTTGA<br /> |rRNA<br /> |-<br /> |TTTTGAGATTCGCTCCACCTTGCGGTATCGCAGCTCATTGTACCGGCCATTGTAGCACGT<br /> |rRNA<br /> |-<br /> |CAATCCGAACTGAGACCGGCTTTTTGAGATTCGCTCCACCTTGCGGTAT<br /> |rRNA<br /> |-<br /> |ATTACTAGCGACTCCGACTTCATGGGGTCGAGTTGCAGACCCCAATCCGAACTGAGA<br /> |rRNA<br /> |-<br /> |TATTCACCGCAGCAATGCTGATCTGCGATTACTAGCGACTCCGACTTCATG<br /> |rRNA<br /> |-<br /> |GTGTACAAGGCCCGGGAACGTATTCACCGCAGCAATGCTG<br /> |rRNA<br /> |-<br /> |GTAATCGGGTGACACCACGAGAGCGGAACAGTCGGAGGAATAA<br /> |rRNA<br /> |-<br /> |GTGTTACCGACTTTCGTGACGTGACGGGCGGTGTGTACAAG<br /> |rRNA<br /> |-<br /> |CACCTTCGACAGCTCCCTCCCACAAGGGGTTGGGCCACCGGCTTCGGGTGTTA<br /> |rRNA<br /> |-<br /> |CTACCTTGTTACGACTTCGTCCCAATCGCCAGTCCCACCTTCGACAG<br /> |rRNA<br /> |-<br /> |TCCTTAGAAAGGAGGTGATCCAGCCGCACCTTCCGGTACGGCTACCTTGTTACGA<br /> |rRNA<br /> |-<br /> |TGCAGTCGGCTATGTGCTCCTTAGAAAGGAGGTGATCCAG<br /> |rRNA<br /> |-<br /> |GATCAAACTCTCCGTGAATGTATACCCGTAATCGGGTGAC<br /> |rRNA<br /> |-<br /> |AAGTGGTTCATCGTTCGACTTGCATGTGTTAAGCACGCCGCCAGCGTTCGTCCTGA<br /> |rRNA<br /> |-<br /> |TATCAACATATCTGGCGTTGACTTTTGGCACGCTGTTGAGTTCTCAAGGAA<br /> |rRNA<br /> |-<br /> |TGTTACTCACCCGTTCGCCACTAATCCCCACCGAAGTGGTTCATCGTTCGACTT<br /> |rRNA<br /> |-<br /> |CTTGTCCCAGAGTGAAGGGCAGATTGCCCACGTGTTACTC<br /> |rRNA<br /> |-<br /> |GCAGATGCCTGCGAGGGTCAGTATCCGGTATTAGACCCCGTTTCCAGGGCTTGTCCCA<br /> |rRNA<br /> |-<br /> |ATCCTTCACCGCCGGAGCTTTCGAACCTCGCAGATGCCTGCGAGGGTCAGTATCC<br /> |rRNA<br /> |-<br /> |GATAGGCCGCGGGCTCATCCTTCACCGCCGGAGCTTTCGA<br /> |rRNA<br /> |-<br /> |GTCGCCTTGGTGAGCCATTACCTCACCAACAAGCTGATAG<br /> |rRNA<br /> |-<br /> |TAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGGCCGGTCGCCCTCTCAGGCCGGCTA<br /> |rRNA<br /> |-<br /> |ATTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTC<br /> |rRNA<br /> |-<br /> |CGTCGCTGCATCAGGCTTTCGCCCATTGTGCAATATTCCC<br /> |rRNA<br /> |-<br /> |CTTCTTCCCTGCTGAAAGAGGTTTACAACCCGAAGGCCGTCATC<br /> |rRNA<br /> |-<br /> |TTCAAAGGAACCTCAACCCGAACGGAGATCCGATCAGGTCGGGGTATCAACATAT<br /> |rRNA<br /> |-<br /> |GTAGTTAGCCGGCGCTTCTTCTGCAGGTACCGTCACTTTC<br /> |rRNA<br /> |-<br /> |ATAATTCCGGACAACGCTTGCGCCCTACGTATTACCGCGGCTGCTGGCACGTAGTTA<br /> |rRNA<br /> |-<br /> |CTTTCACAACCGACGTGACAAGCCGCCTACGAGCTCTTTACGCCCAATAATT<br /> |rRNA<br /> |-<br /> |TATCGACTGCAGACCCGGGGTTAAGCCCCGGGCTTTCACAACCGACGTGACAAG<br /> |rRNA<br /> |-<br /> |GATCTCCCCTACCGAACTCTAGCCTGCCCGTATCGACTGCAGAC<br /> |rRNA<br /> |-<br /> |CTGATATCTGCGCATTTCACCGCTACACCAGGAATTCCGATCTC<br /> |rRNA<br /> |-<br /> |CAGAGATCCGCCTTCGCCACCGGTGTTCCTCCTGATATCTG<br /> |rRNA<br /> |-<br /> |CACGCTTTCGCTCCTCAGCGTCAGTATCGGCCCAGAGATC<br /> |rRNA<br /> |-<br /> |CACCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTTC<br /> |rRNA<br /> |-<br /> |ACTTAATGCGTTAGCTGCGGCACGGACAACGTGGAATGTTGCCCACACCTAGTGCCCA<br /> |rRNA<br /> |-<br /> |GTTCGAAATGTAACCGGGCGTTTCCCCTACGCTATGACCACCGAAA<br /> |rRNA<br /> |-<br /> |CTTAGGATGGTTATAGTTACCACCGCCGTTTACTGGCGCTTAAGTTCTCA<br /> |rRNA<br /> |-<br /> |CCAAGCAAGCTTGATGTCCGACGCTCCCACGGCTTGTAGGCACACGGTTTCAGGTACTA<br /> |rRNA<br /> |-<br /> |CAGTCACGAGACACCAAGCAAGCTTGATGTCCGACGCTCC<br /> |rRNA<br /> |-<br /> |CTTGAGCGTGCTACTAAAAGCGCCCTATTCGGACTCGCTT<br /> |rRNA<br /> |-<br /> |CTACCTTGTTACGACTTCGTCCCAATCGCCAGTCCCACCTTCGACAGCTCCCT<br /> |rRNA<br /> |-<br /> |AAGGAGGTGATCCAGCCGCACCTTCCGGTACGGCTACCTTGTTACGACTTCGTC<br /> |rRNA<br /> |-<br /> |CCAGGATCAAACTCTCCGTGAATGTATACCCGTAATCGGG<br /> |rRNA<br /> |-<br /> |CTACCTTGTTACGACTTCGTCCCAATCGCCAGTCCCACCTTCG<br /> |rRNA<br /> |-<br /> |CTCTCACTTACCGGCATTCGGAGTTTGGCTAAGGTCAGTAAC<br /> |rRNA<br /> |-<br /> |CATTCGGACACCCCCGGATCAAAGCCTGGTTGACGACTCC<br /> |rRNA<br /> |-<br /> |GCTTATCCCCCACAGTCTCACTGCCGCGCTCTCACTTACC<br /> |rRNA<br /> |-<br /> |CTTAGTCGATGCTCTGGGCTGTTTCCCTCTCGACCATGGAGCTTATC<br /> |rRNA<br /> |-<br /> |CACATCCTTTCCCACTTAGCGTACGCTTAGGGGCCTTAGTCGATGCTCTG<br /> |rRNA<br /> |-<br /> |CTTCTAAGCCAACCTCCTGGTTGTCTGTGCGACTCCACATCCTTTC<br /> |rRNA<br /> |-<br /> |AATCACTAGACCAGTGAGCTATTACGCACTCTTTCAAGGGTGGCTGCTTCTAAGC<br /> |rRNA<br /> |-<br /> |CTACATTGTCGGCGCGGAATCACTAGACCAGTGAGCTATTA<br /> |rRNA<br /> |-<br /> |CGTATTGCTGCAATGACACGACTTCGGCGGTACGCTTGAG<br /> |rRNA<br /> |-<br /> |GCTCCCCTACCCATCACAGCCGGCGTTGGCCGTATTGCTG<br /> |rRNA<br /> |-<br /> |TTCTCACTCGTGAACCGTCCACAACTCGCTTCCGCGGCTGCTTCACCCGGCACACGACG<br /> |rRNA<br /> |-<br /> |GTTTGTATCGCTACTCATGCCTGCATTCTCACTCGTGAACCGTCCACAAC<br /> |rRNA<br /> |-<br /> |CTTAGTCAATCGGCGCAAACGTTTCTCACGTTTGTATCGCTACTCATG<br /> |rRNA<br /> |-<br /> |GACTTACCCTGGGCAGATCAGCTTGACCCAGGAACCCTTAGTCAATC<br /> |rRNA<br /> |-<br /> |TCCACACTGCCTATGTGTTCAGCAGCGGGTGACAGCCCATGACGACTGCCGGGTTT<br /> |rRNA<br /> |-<br /> |TTATCCATCGACTACGCCTGTCGGCCTCGCCTTAGGTCCCGACTTACCCTGGGCA<br /> |rRNA<br /> |-<br /> |GTACCGGAATATCAACCGGTTATCCATCGACTACGCCTGTC<br /> |rRNA<br /> |-<br /> |GGCCTTAGCATCACGATGCTCGATGTTTGACGCTTCACAG<br /> |rRNA<br /> |-<br /> |CTACTAACCGCTTGGTTCGGCGGCTCCACCACTCCCCTCAACTCCGAAGAGATCA<br /> |rRNA<br /> |-<br /> |GATGGACTACCTTCCTGCGTCACCCCATCACTCACCTACTAAC<br /> |rRNA<br /> |-<br /> |CCGGCCTACACCCTTACCCCGGGACAACCACCGCCCGGGATGGACTACC<br /> |rRNA<br /> |-<br /> |CAAGGCATCCACCGTGCGCCCTTAAAAACTTGGCCACAGA<br /> |rRNA<br /> |-<br /> |CAAGGTGTGCGGATTTACCTACACACCGGCCTACACCCTTAC<br /> |rRNA<br /> |-<br /> |CCACAATCGGCTCGGCATCAGGTCTCAGCCACAAGGTGTG<br /> |rRNA<br /> |-<br /> |CTAGAGGCTTTTCTCGACAGCATAGGATCATCCACTTCACCACAATC<br /> |rRNA<br /> |-<br /> |TTTAGGGTACGGGCCGCCATGAAACTCGCTAGAGGCTTTTCTC<br /> |rRNA<br /> |-<br /> |CTCGGTATTCTCTACCTGACCACCTGAGTCGGTTTAGGGTAC<br /> |rRNA<br /> |-<br /> |GGCATTTTGCCGAGTTCCTTAACCATAGTTCACCCGAACG<br /> |rRNA<br /> |-<br /> |CAGCTCAGAGTGCAAAACTCGTCACCAGGTGTGGCCCCCCTTCTCCCGAAGTTA<br /> |rRNA<br /> |-<br /> |TTGTTTTCTCTTCCTGCGGGTACTGAGATGTTTCACTTCCCCGCGTTCCCTCCACA<br /> |rRNA<br /> |-<br /> |TGTGTTTTTAGTAAACAGTCGCTTCTCGCTGGTCTCTGCGGCCACCCCCAGCTCAGA<br /> |rRNA<br /> |-<br /> |TATACATCGCCTTACGGCTTCGCACGGACCTGTGTTTTTAGTAAACAGTCGCTTCT<br /> |rRNA<br /> |-<br /> |GCAGGCGTCAGTCCGTATACATCGCCTTACGGCTTCGCAC<br /> |rRNA<br /> |-<br /> |GAAATGTGACTAACCGGTCCCCTTAACGTTCCAGCACCGGGCAGGC<br /> |rRNA<br /> |-<br /> |GTTTACTGGCGCTTAAGTTCTCAGCTTCGCCACACCGAAATGTGACTAAC<br /> |rRNA<br /> |-<br /> |GACAAGGAATTTCGCTACCTTAGGATGGTTATAGTTACCACCGCCGTTTA<br /> |rRNA<br /> |-<br /> |AAGTCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTA<br /> |rRNA<br /> |-<br /> |CTATGGTTGAGACAGTCGAGAAGTCGTTACGCCATTCGTG<br /> |rRNA<br /> |-<br /> |CTGCGCGAAACGAGCATCTTTACTCGTAGTGCAATTTCAC<br /> |rRNA<br /> |-<br /> |CAATATCAAACTGTAGTAAAGGTCCCGGGGTCTTTCCGTCCTGCTGCGCGAAA<br /> |rRNA<br /> |-<br /> |CAGCTATCCTACACAAGCCGAACCGAACACCAATATCAAACTGTAGTAAA<br /> |rRNA<br /> |-<br /> |CACACTGGCGTGCGAGCTTCAAAGTCTCCCAGCTATCCTACACAAG<br /> |rRNA<br /> |-<br /> |TTAGACATCCAGCACGACCAGACTGGTATTTCAACGACGACTCCACCCACACTGGCGTG<br /> |rRNA<br /> |-<br /> |GTTTCGCTCGCCACTACTCCCGGAATCACGGTTGTTTTCTCTTC<br /> |rRNA<br /> |-<br /> |GATCACGGACCCAGGTTAGACATCCAGCACGACCAGACTG<br /> |rRNA<br /> |-<br /> |CAGTTAAACTACCCATCAGACACTGTCCCTGATCCGGATCAC<br /> |rRNA<br /> |-<br /> |GAACCTTTGGGCGCCTCCGTTACTCTTTAGGAGGCAACCG<br /> |rRNA<br /> |-<br /> |CTTGTGCACTTACACTCAACACCTGATTGCCAACCAGGCTGAGGGAACCTTT<br /> |rRNA<br /> |-<br /> |GACCCGTCGGTCTCACAGTCAAGCTCCCTTGTGCACTTAC<br /> |rRNA<br /> |-<br /> |CTTCCACAAGCCACCGCCGGATCACTAGTCCCGACTTTCGTCCCTG<br /> |rRNA<br /> |-<br /> |GTACCTTTTATCCGTTGAGCGACGGCGCTTCCACAAGCCAC<br /> |rRNA<br /> |-<br /> |CAAACCATCCCGTCGATATGGACTCTTGGGGAAGATCAGCCTGTTATC<br /> |rRNA<br /> |-<br /> |CCAGGATGCGACGAGCCGACATCGAGGTGCCAAACCATCC<br /> |rRNA<br /> |-<br /> |GTCTCACACGTATACGGTTTGGCCTCATCCGGTTTCGCTC<br /> |rRNA<br /> |-<br /> |CTTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTCCAG<br /> |rRNA<br /> |-<br /> |GAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCGCTTTAATG<br /> |rRNA<br /> |-<br /> |CTTCTCAAGACTCCTACGCGCACAGCGGATAGGGACCGAAC<br /> |rRNA<br /> |-<br /> |CACACCAGAGGTTCGTCCGTCCCGGTCCTCTCGTACTAGG<br /> |rRNA<br /> |-<br /> |TTAGATGCTTTCAGCGGTTATCCCTCCCGAACGTAGCCAACCAGCCATGCCCTTGGCA<br /> |rRNA<br /> |-<br /> |CTCATCTCGAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATC<br /> |rRNA<br /> |-<br /> |CAGTCGTCTACTGGGAGCCTTAACCCCTCAAGGGGGTGGGAGTCCTCATCTCGAA<br /> |rRNA<br /> |-<br /> |CTTCCAGATCCGGCCTATCAACCCAGTCGTCTACTGGGAG<br /> |rRNA<br /> |-<br /> |CAGACCGTAAAAGAGAGATCCCACAACCCCGCACACGCAACCCCTGCCGGGTCTCA<br /> |rRNA<br /> |-<br /> |TACATCAACGGTTTCTGACTCGTCTCACGGCCGGCAGACCGTAAAAGAGAGAT<br /> |rRNA<br /> |-<br /> |CTTTCGCATGTCCTTCGCCTACATCAACGGTTTCTGACTC<br /> |rRNA<br /> |-<br /> |ACTACGGGGGTCTTACCCTCTACGCCGGACCTTTCGCATG<br /> |rRNA<br /> |-<br /> |CTACTTGGGTGTCTCTCAAACGAGCCGTTGACGTTTCGACTAC<br /> |rRNA<br /> |-<br /> |GCCAGATTCACACGGGATTTCTCGGGCCCCGTGCTACTTG<br /> |rRNA<br /> |-<br /> |TATCCGCTATCGGTCACCAGGGAATATTTAGGCTTAGCGGGTGGTCCCGCCAGATT<br /> |rRNA<br /> |-<br /> |GTACTTTTCACCATTCCCTCACGGTACTATCCGCTATCGGTCAC<br /> |rRNA<br /> |-<br /> |CTTGTAGGCACACGGTTTCAGGTACTATTTCACTCCGCTCCC<br /> |rRNA<br /> |-<br /> |GGGCCTATCGTGGCCTCCCACGTCCTTCATCGGTTCCTGG<br /> |rRNA<br /> |-<br /> |CACGCAGTCACGAGGATGGAGCAAGCTCCATCCCGACGCTCCCACGGCTTGTAGGCA<br /> |rRNA<br /> |-<br /> |CATTCTTCAAAAGGCACGCAGTCACGAGGATGGAGCAAGC<br /> |rRNA<br /> |-<br /> |TACGGCTTCCCCACTCGGGTTAACCTCGCAACACACCGCAAACTCGCAGGCTCAT<br /> |rRNA<br /> |-<br /> |TCGGACTCGCTTTCGCTACGGCTTCCCCACTCGGGTTAAC<br /> |rRNA<br /> |-<br /> |ATCACTCCGCTTCGGGTCTTGAGCGTGCTACTAAAAACGC<br /> |rRNA<br /> |-<br /> |CTCTTACAGCCGCTTCAACCTGCCCATGGCTAGATCACTC<br /> |rRNA<br /> |-<br /> |GTTTTCAACCCTGGTGGGTTCGGTCCTCCACGACCTCTTAC<br /> |rRNA<br /> |-<br /> |GTTTGATTGGCCTTTCACCCCTAACCACAGGTCATCCCCCAGGTTTTCAACC<br /> |rRNA<br /> |-<br /> |GGAGAACCAGCTATCACGGAGTTTGATTGGCCTTTCACCC<br /> |rRNA<br /> |-<br /> |TATCCAGTGCTCTACCTCCGGCAAGAAACACACGACGCTGCACCTAAATGCATTT<br /> |rRNA<br /> |-<br /> |GAGTTTGGCTAAGGTCAGTAACCCGGTAGGGCCCATCGCCTATCCAGTGCTCTA<br /> |rRNA<br /> |-<br /> |CTGTAAGGCTTAGCTTCCGGGTTCGAAATGTAACCGGGCGTTTCCCCTAC<br /> |rRNA<br /> |-<br /> |GTTCGGCGGCGTCCTACTCTCCCACAGGGTCCCCCCTGCAGTACCATCGGCGCTGTA<br /> |rRNA<br /> |-<br /> |GTATTAGACCCCGTTTCCAGGGCTTGTCCCAGAGTGAAGGGCAGATT<br /> |rRNA<br /> |-<br /> |GAGCTTTCGAACCTCGCAGATGCCTGCGAGGGTCAGTATCCGGTATTAGACCCCGTT<br /> |rRNA<br /> |-<br /> |CATCCTTCACCGCCGGAGCTTTCGAACCTCGCAGATGCCT<br /> |rRNA<br /> |-<br /> |CACCGAAGTGGTTCATCGTTCGACTTGCATGTGTTAAGCAC<br /> |rRNA<br /> |-<br /> |TTCGACTTGCATGTGTTAAGCACGCCGCCAGCGTTCGTCCTGAGCCAGGATCAAACTCT<br /> |rRNA<br /> |-<br /> |CAGATTGCCCACGTGTTACTCACCCGTTCGCCACTAATCC<br /> |rRNA<br /> |-<br /> |GCGGATTTACCTACCTGACGTCCTACACCCTTACCCCGGGACAACCACCGC<br /> |rRNA<br /> |-<br /> |CATCAGGTCTCAGACTCATGTCAGGCGGATTTACCTACCTGAC<br /> |rRNA<br /> |-<br /> |CTATTAGTACCGGTCACCTCCACACCTCACGGTGCTTCCAGATCCGGCCTATCAACC<br /> |rRNA<br /> |-<br /> |GGACAAGCCCTCGGCCTATTAGTACCGGTCACCTCCACAC<br /> |rRNA<br /> |-<br /> |CCGGGTTAACCTCGCAACACACCGCAAACTCGCAGGCTCATTCTTCAAAAGGCACGC<br /> |rRNA<br /> |-<br /> |GGTCTTGAGCGTGCTACTAAAACGCCCTATTCGGACTCGC<br /> |rRNA<br /> |-<br /> |TTCGGCGGCGTCCTACTCTCCCACAGGGTCCCCCCTGCAGTACCATCGGCGCTGTAA<br /> |rRNA<br /> |-<br /> |CCGAAGCCTCCGGCGGTCTGCTTTCTGTGGCACTGTCCCG<br /> |SCOs01<br /> |-<br /> |CTGGGCACTGGTGGTCTCTTACACCACCGTTTCACCCTTAC<br /> |SCOs01<br /> |-<br /> |TTCCTTCTTGACCTTGCTCCGGGTGGGGTTTACCTAGCTGCCCAGGTCGCCCTG<br /> |SCOs01<br /> |-<br /> |CTCGAACGTCCGCGCAGAAGCACCGGGGTGCTTCCTTCTTGACCTTGCTC<br /> |SCOs01<br /> |-<br /> |CTCCAGCGGTCTACCCGCGGACTCGGGCGGGCAGCCCTCGAACGTCCGCGCAGAAG<br /> |SCOs01<br /> |-<br /> |CATCCATCTAGGACCGGCGTTGCCACCGGCCTCCAGCGGTCTAC<br /> |SCOs01<br /> |-<br /> |CTGTGGAGCCCGGACGTTCCTCGGGAAGTCCCGTGAGGGACTC<br /> |SCOs01<br /> |-<br /> |GTTCTGTTCCGACGGGGCCTCGCGGTCCCGTCGGCGACGGCCATCCAT<br /> |SCOs01<br /> |-<br /> |CAGACGAGTCGGGCTGTACGCCGGGTTCTGTTCCGACGGG<br /> |SCOs01<br /> |-<br /> |GTCTGCTTTCTGTGGCACTGTCCCGCGGGTCACCCCGGGTGGCCGTTAGCCATCAC<br /> |SCOs01<br /> |-<br /> |CTTAGTAGGAGGCTCGCTTCGCTACCTGAGATCAGGCAGCGAGGGCGAAGG<br /> |SCOs02<br /> |-<br /> |GAACAGCCCCGGGCTGACACTCCCTTAGTAGGAGGCTCGCTTC<br /> |SCOs02<br /> |-<br /> |GATCAAGGTTTAGTCCCCTAGCTGATGCCAGGATCCGGGTC<br /> |SCOs02<br /> |-<br /> |CAGTCACTGTTTGGTTTCCCTCTTCACCCCGTGACCGGGATCAA<br /> |SCOs02<br /> |-<br /> |GAGATCACGCGGACAAGTCTCCGACGGGCCCAGTCACTGT<br /> |SCOs02<br /> |-<br /> |CTTCCCCTGCTTCGACAGCCGCTGTCGAAACCGATCATCC<br /> |SCOs02<br /> |-<br /> |AATCAGGGCTTCTCCGTGTGCAGTCCGCTTCGATTTTCTCGGCCCCGGAGATCACG<br /> |SCOs02<br /> |-<br /> |GAATCGAACCCGCGTCCAACGGTGCGGAATCAGGGCTTCTC<br /> |SCOs02<br /> |-<br /> |TTTTTCGGCCTGTGGTTTACGAGATCATGGCCGCTTCCTC<br /> |SCOs02<br /> |-<br /> |CTGCTGGGAGGAATCGCGCTTGGTGTTGGCGATTATTTTT<br /> |SCOs02<br /> |-<br /> |CACGCACCCGCCAGAGCCGACCTACCCTTGCTGCCTTCCG<br /> |SCOs03<br /> |}</div> RTEvans https://actinobase.org/index.php?title=RRNA_depletion_with_Streptomyces_coelicolor_A3(2)_specific_probes&diff=2195 RRNA depletion with Streptomyces coelicolor A3(2) specific probes 2023-11-01T16:49:10Z <p>RTEvans: /* Description */</p> <hr /> <div>==Organisms==<br /> <br /> This protocol has been tested with &lt;em&gt;[[Streptomyces coelicolor]]&lt;/em&gt; A3(2) MT1110<br /> <br /> ==Description==<br /> <br /> rRNA depletion using commercial kits targeting generic bacterial sequences is not 100% efficient in the High GC organism &lt;em&gt;S. coelicolor&lt;/em&gt;. <br /> <br /> Depletion using the NEBNext RNA Depletion Core Reagent Set with RNA Sample Purification Beads (https://www.neb.com/en-gb/products/e7870-nebnext-rna-depletion-core-reagent-set-with-rna-sample-purification-beads#Product%20Information) and custom DNA probes designed for &lt;em&gt;S. coelicolor&lt;/em&gt; is more effective than the QIAseq FastSelect 5S/16S/23S kit (Qiagen #335927); NEBNext rRNA Depletion Kit (Bacteria) (NEB #E7850); Illumina Ribo-zero rRNA Removal Kit (Bacteria) (Illumina #MRZMB12424, discontinued). The Illumina kit tested was subsequently replaced by the Illumina Ribo-Zero Plus rRNA Depletion Kit (Illumina #20040526). Additionally three abundant non-mRNA RNAs can be depleted (Table 1).<br /> <br /> '''Table 1.''' Abundant non-mRNA RNAs in &lt;em&gt;S. coelicolor&lt;/em&gt;.<br /> <br /> [[File: rRNA_depletion_table_1.png]]<br /> <br /> ==Materials==<br /> <br /> Single stranded DNA probes (Table 2) at 2µM concentration in IDTE pH 7.5 (10mM Tris-HCl / 0.1mM EDTA). Integrated DNA Technologies can produce these from their 25nmole DNA oligo pool product. Probes are listed in Table 2 in the Notes section below.<br /> <br /> NEBNext RNA Depletion Core Reagent Set with RNA Sample Purification Beads (https://www.neb.com/en-gb/products/e7870-nebnext-rna-depletion-core-reagent-set-with-rna-sample-purification-beads#Product%20Information).<br /> <br /> ==Protocols==<br /> <br /> As per manufacturer’s instructions.<br /> <br /> ==Notes==<br /> <br /> The following histograms illustrate effectiveness of rRNA depletion using various methods. Histograms show percentage of bases aligned to NCBI reference sequence NC_003888.3 using Hisat2 program classified as follows - Coding = RNA aligned to coding sequences; Transcribed not translated = RNA aligned to rRNA, tRNA and sRNA; Intergenic = RNA aligned to 5' or 3' UTRs or between annotated sequences.<br /> <br /> '''QIAseq FastSelect 5S/16S/23S kit'''<br /> <br /> [[File: PanelA.png]] <br /> <br /> '''NEBNext rRNA Depletion Kit (Bacteria)'''<br /> <br /> [[File: PanelB.png]]<br /> <br /> '''Illumina Ribo-zero rRNA Removal Kit (Bacteria)'''<br /> <br /> [[File: PanelC.png]] <br /> <br /> '''rRNA depletion with &lt;em&gt;S. coelicolor&lt;/em&gt; specific probes<br /> <br /> [[File: PanelD.png]]<br /> <br /> <br /> '''Table 2''' List of probes<br /> <br /> {| class=&quot;wikitable&quot;<br /> !Sequence<br /> !Target<br /> |-<br /> |CAAGTCCTTGCGCGACGCTCCCACGGCTTGTAGGCACACGGTTTCAGGTACTATTT<br /> |rRNA<br /> |-<br /> |CAGTCACGACGCAAGGACAAGTCCTTGCGCGACGCTCCCAC<br /> |rRNA<br /> |-<br /> |ACCTCGCAACACACCGCAAACTCGCAGGCTCATTCTTCAAAAGGCACGCAGTCACGACG<br /> |rRNA<br /> |-<br /> |CTACGGCTTCCCCACCCGGGTTAACCTCGCAACACACCGC<br /> |rRNA<br /> |-<br /> |TTAACCCAACATCTCACGACACGAGCTGACGACAGCCATGCACCACCTGTACACCGA<br /> |rRNA<br /> |-<br /> |CGCTCGTTGCGGGACTTAACCCAACATCTCACGACACGAG<br /> |rRNA<br /> |-<br /> |CAACACCCCGAAGGGCTTGCTGGCAACACGGGACAAGGGTTG<br /> |rRNA<br /> |-<br /> |CACCTTCCTCCGAGTTGACCCCGGCGGTCTCCCGTGAGTC<br /> |rRNA<br /> |-<br /> |GACATAAGGGGCATGATGACTTGACGTCGTCCCCACCTTC<br /> |rRNA<br /> |-<br /> |GTATCGCAGCTCATTGTACCGGCCATTGTAGCACGTGTGCAGCCCAAGACATAA<br /> |rRNA<br /> |-<br /> |TTTTGAGATTCGCTCCACCTTGCGGTATCGCAGCTCATTGTAC<br /> |rRNA<br /> |-<br /> |CAGACCCCAATCCGAACTGAGACCGGCTTTTTGAGATTCG<br /> |rRNA<br /> |-<br /> |AATGCTGATCTGCGATTACTAGCGACTCCGACTTCATGGGGTCGAGTTGCAGAC<br /> |rRNA<br /> |-<br /> |GAACGTATTCACCGCAGCAATGCTGATCTGCGATTACTAG<br /> |rRNA<br /> |-<br /> |TACCGACTTTCGTGACGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTC<br /> |rRNA<br /> |-<br /> |GCCACCGGCTTCGGGTGTTACCGACTTTCGTGACGTGACG<br /> |rRNA<br /> |-<br /> |TTCGTCCCAATCGCCAGTCCCACCTTCGACAGCTCCCTCCCACAAGGGGTTGGG<br /> |rRNA<br /> |-<br /> |CGCACCTTCCGGTACGGCTACCTTGTTACGACTTCGTCCCAATCGCCAGTCCCA<br /> |rRNA<br /> |-<br /> |TTCGACCCTCGCAGATGCCTGCGAGGGTCAGTATCCGGTATTAGACCCCGTTTCCAG<br /> |rRNA<br /> |-<br /> |GAAAGGAGGTGATCCAGCCGCACCTTCCGGTACGGCTACC<br /> |rRNA<br /> |-<br /> |TTCACCGCCGGAGCTTTCGACCCTCGCAGATGCCTGCGAG<br /> |rRNA<br /> |-<br /> |CATTACCTCACCAACAAGCTGATAGGCCGCGGGCTCATCCTTC<br /> |rRNA<br /> |-<br /> |CTCTCAGGCCGGCTACCCGTCGTCGCCTTGGTGAGCCATTAC<br /> |rRNA<br /> |-<br /> |GTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGGCCGGTCG<br /> |rRNA<br /> |-<br /> |TGCATCAGGCTTTCGCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCT<br /> |rRNA<br /> |-<br /> |TGCTGAAAGAGGTTTACAACCCGAAGGCCGTCATCCCTCACGCGGCGTCGCTGCATCA<br /> |rRNA<br /> |-<br /> |TTCTTCTGCAGGTACCGTCACTTTCGCTTCTTCCCTGCTGAAAGAGGTTTACAA<br /> |rRNA<br /> |-<br /> |CTACGTATTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTCTG<br /> |rRNA<br /> |-<br /> |CTCTTTACGCCCAATAATTCCGGACAACGCTTGCGCCCTACGTATTAC<br /> |rRNA<br /> |-<br /> |GGGCTTTCACAACCGACGTGACAAGCCGCCTACGAGCTCT<br /> |rRNA<br /> |-<br /> |CTACCGAACTCTAGCCTGCCCGTATCGACTGCAGACCCGGGGTTAAGCCCCG<br /> |rRNA<br /> |-<br /> |ATATCTGCGCATTTCACCGCTACACCAGGAATTCCGATCTCCCCTACCGAACTCTAG<br /> |rRNA<br /> |-<br /> |CGGTGTTCCTCCTGATATCTGCGCATTTCACCGCTACACC<br /> |rRNA<br /> |-<br /> |ACGCTTTCGCTCCTCAGCGTCAGTATCGGCCCAGAGATCCGCCTTCGCCACCGGTGT<br /> |rRNA<br /> |-<br /> |GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCG<br /> |rRNA<br /> |-<br /> |ACAACGTGGAATGTTGCCCACACCTAGTGCCCACCGTTTACGGCGTGGACTACCA<br /> |rRNA<br /> |-<br /> |CTTAATGCGTTAGCTGCGGCACGGACAACGTGGAATGTTG<br /> |rRNA<br /> |-<br /> |TCAATTCCTTTGAGTTTTAGCCTTGCGGCCGTACTCCCCAGGCGGGGCACTTAA<br /> |rRNA<br /> |-<br /> |ATTAAGCCACATGCTCCGCCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTTA<br /> |rRNA<br /> |-<br /> |TCAAGCCTTGGTAAGGTTCTTCGCGTTGCGTCGAATTAAGCCACATGCTCCGCCGCTT<br /> |rRNA<br /> |-<br /> |CATCTCTGATGCTTTCCGGTGTATGTCAAGCCTTGGTAAGGTTCTTC<br /> |rRNA<br /> |-<br /> |ACAGCCATGCACCACCTGTACACCGACCACAAGGGGGGCACCATCTCTGATGCTTTCC<br /> |rRNA<br /> |-<br /> |TGTAACCGGGCGTTTCCCCTACGCTATGACCACCGAAACC<br /> |rRNA<br /> |-<br /> |CAGTACCATCGGCGCTGTAAGGCTTAGCTTCCGGGTTCGAAATGTAAC<br /> |rRNA<br /> |-<br /> |GTTCGGCGGCGTCCTACTCTCCCACAGGGTCCCCCCTGCA<br /> |rRNA<br /> |-<br /> |CACTCCCCTCAACTCCGAAGAGATCAGGGCGGCTTCACGG<br /> |rRNA<br /> |-<br /> |CCATCACTCACCTACTAACCGCTTGGTTCGGCGGCTCCAC<br /> |rRNA<br /> |-<br /> |GTCCTTCATCGGTTCCTGGTGCCAAGGCATCCACCGTGCGCCCTTAAAAACTT<br /> |rRNA<br /> |-<br /> |CTTGTAGGCACACGGTTTCAGGTACTATTTCACTCCGCTCCCG<br /> |rRNA<br /> |-<br /> |CACGCAGTCACGAGACACCAAGCAAGCTTGATGTCCGACGCTCCCACGGCTTGTAGGCAC<br /> |rRNA<br /> |-<br /> |TCTTCAAAAGGCACGCAGTCACGAGACACCAAGCAAGCTT<br /> |rRNA<br /> |-<br /> |CACCCGGGTTAACCTCGCAACACACTGCAAACTCGCAGGC<br /> |rRNA<br /> |-<br /> |GCGTGCTACTAAAACGCCCTATTCGGACTCGCTTTCGCTACGGCTTCC<br /> |rRNA<br /> |-<br /> |GATCACTCCGCTTCGGGTCTTGAGCGTGCTACTAAAACGC<br /> |rRNA<br /> |-<br /> |TCGCAGATGCCTGCAAGGGTCAGTATCCGGTATTAGACCCCGTTT<br /> |rRNA<br /> |-<br /> |TCATCCTTCACCGCCGCAGCTTTCGAACCTCGCAGATGCC<br /> |rRNA<br /> |-<br /> |ATGTAACCGGGCGTTTCCCCTACGCTATGACCACCGAAAC<br /> |rRNA<br /> |-<br /> |CGTCCTACTCTCCCACAGGGTCCCCCCTGCAGTACCATCG<br /> |rRNA<br /> |-<br /> |CATCGCCTATCCAGTGCTCTACCTCCGGCAAGAAACACAC<br /> |rRNA<br /> |-<br /> |CATTCGGAGTTTGGCTAAGGTCAGTAACCCGGTAGGGCCCATCG<br /> |rRNA<br /> |-<br /> |CAGTCTCACTGCCGCGCTCTCACTTACCGGCATTCGGAGTTTG<br /> |rRNA<br /> |-<br /> |ATGCTCTGGGCTGTTTCCCTCTCGACCATGGAGCTTATCCCCCACAGTCTCACTG<br /> |rRNA<br /> |-<br /> |CTTAGGGGCCTTAGTCGATGCTCTGGGCTGTTTCCCTCTCG<br /> |rRNA<br /> |-<br /> |GTTGTCTGTGCGACTCCACATCCTTTCCCACTTAGCGTACGCTTAG<br /> |rRNA<br /> |-<br /> |TATTACGCACTCTTTCAAGGGTGGCTGCTTCTAAGCCAACCTCCTGGTTGTCTGT<br /> |rRNA<br /> |-<br /> |GATCAAAGCCTGGTTGACGACTCCCCGGGGCCTATCGTGG<br /> |rRNA<br /> |-<br /> |CGGAATCACTAGACCAGTGAGCTATTACGCACTCTTTCAAGG<br /> |rRNA<br /> |-<br /> |TATTGCTGCAATGACACGACTTCGGCGGTACGCTTGAGCCCCGCTACATTGTCG<br /> |rRNA<br /> |-<br /> |CATCACAGCCGGCGTTGGCCGTATTGCTGCAATGACACGACTTC<br /> |rRNA<br /> |-<br /> |TCCACAACTCGCTTCCGCGGCTGCTTCACCCGGCACACGACGCTCCCCTACCCATCACA<br /> |rRNA<br /> |-<br /> |TACTCATGCCTGCATTCTCACTCGTGAACCGTCCACAACTCGCTTCCGCGGCTGCTT<br /> |rRNA<br /> |-<br /> |CAAACGTTTCTCACGTTTGTATCGCTACTCATGCCTGCATTCTCACTC<br /> |rRNA<br /> |-<br /> |CAGGAACCCTTAGTCAATCGGCGCAAACGTTTCTCACGTTT<br /> |rRNA<br /> |-<br /> |CTTAGGTCCCGACTTACCCTGGGCAGATCAGCTTGACCCAG<br /> |rRNA<br /> |-<br /> |GAATATCAACCGGTTATCCATCGACTACGCCTGTCGGCCTCGCCTTAGGTC<br /> |rRNA<br /> |-<br /> |TTAGCATCACGATGCTCGATGTTTGACGCTTCACAGCGGGTACCGGAATATCAA<br /> |rRNA<br /> |-<br /> |GAAGAGATCAGGGCGGCTTCACGGCCTTAGCATCACGATG<br /> |rRNA<br /> |-<br /> |CATCACTCACCTACTAACCGCTTGGTTCGGCGGCTCCACC<br /> |rRNA<br /> |-<br /> |TGTGTTCAGCAGCGGGTGACAGCCCATGACGACTGCCGGGTTTCCCCATTCGGACACCC<br /> |rRNA<br /> |-<br /> |GACAACCACCGCCCGGGATGGACTACCTTCCTGCGTCACCCCAT<br /> |rRNA<br /> |-<br /> |CAGATGCTCGCGTCCACTGTGCAGTTCTCAAACAACGACC<br /> |rRNA<br /> |-<br /> |TGCGGATTTACCTACACACCGGCCTACACCCTTACCCCGGGACAACCACCG<br /> |rRNA<br /> |-<br /> |CATCAGGTCTCAGCCACAAGGTGTGCGGATTTACCTACAC<br /> |rRNA<br /> |-<br /> |TTTTCTCGACAGCATAGGATCATCCACTTCACCACAATCGGCTCGGCATCAGGTCT<br /> |rRNA<br /> |-<br /> |CCATGAAACTCGCTAGAGGCTTTTCTCGACAGCATAGGATCATC<br /> |rRNA<br /> |-<br /> |TATTCTCTACCTGACCACCTGAGTCGGTTTAGGGTACGGGCCGCCATGAAACT<br /> |rRNA<br /> |-<br /> |GAGTTCCTTAACCATAGTTCACCCGAACGCCTCGGTATTCTCTACCTGAC<br /> |rRNA<br /> |-<br /> |CTTCTCCCGAAGTTACGGGGGCATTTTGCCGAGTTCCTTAACCA<br /> |rRNA<br /> |-<br /> |CACCCCCAGCTCAGAGTGCAAAACTCGTCACCAGGTGTGG<br /> |rRNA<br /> |-<br /> |GTGTTTTTAGTAAACAGTCGCTTCTCGCTGGTCTCTGCGG<br /> |rRNA<br /> |-<br /> |GTTCCCTCCACACTGCCTATGTGTTCAGCAGCGGGTGACAG<br /> |rRNA<br /> |-<br /> |GTATACATCGCCTTACGGCTTCGCACGGACCTGTGTTTTTA<br /> |rRNA<br /> |-<br /> |CTTAACGTTCCAGCACCGGGCAGGCGTCAGTCCGTATACATC<br /> |rRNA<br /> |-<br /> |TAAGTTCTCAGCTTCGCCACACCGAAATGTGACTAACCGGTCCCCTTAACGTT<br /> |rRNA<br /> |-<br /> |TTAGGATGGTTATAGTTACCACCGCCGTTTACTGGCGCTTAAGTTCTCAG<br /> |rRNA<br /> |-<br /> |GTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGATGGTTATAGT<br /> |rRNA<br /> |-<br /> |TATGGTTGAGACAGTCGAGAAGTCGTTACGCCATTCGTGCAGGTCGGAACTTACC<br /> |rRNA<br /> |-<br /> |GAAACGAGCATCTTTACTCGTAGTGCAATTTCACCGGGCCTATGGTTGAG<br /> |rRNA<br /> |-<br /> |GGTCTTTCCGTCCTGCTGCGCGAAACGAGCATCTTTACTC<br /> |rRNA<br /> |-<br /> |GAACCGAACACCAATATCAAACTGTAGTAAAGGTCCCGGGGTCTTTC<br /> |rRNA<br /> |-<br /> |CTTCAAAGTCTCCCAGCTATCCTACACAAGCCGAACCGAACACCAATA<br /> |rRNA<br /> |-<br /> |AGACTGGTATTTCAACGACGACTCCACCCACACTGGCGTGCGAGCTTCAAAGTCT<br /> |rRNA<br /> |-<br /> |GTTAGACATCCAGCACGACCAGACTGGTATTTCAACGACGACTC<br /> |rRNA<br /> |-<br /> |TTAAACTACCCATCAGACACTGTCCCTGATCCGGATCACGGACCCAGGTTAGACAT<br /> |rRNA<br /> |-<br /> |GAATCACGGTTGTTTTCTCTTCCTGCGGGTACTGAGATGTTTCACTTC<br /> |rRNA<br /> |-<br /> |GTTACTCTTTAGGAGGCAACCGCCCCAGTTAAACTACCCATCAGACACTG<br /> |rRNA<br /> |-<br /> |ACTCAACACCTGATTGCCAACCAGGCTGAGGGAACCTTTGGGCGCCTCCGTTACT<br /> |rRNA<br /> |-<br /> |GTCTCACAGTCAAGCTCCCTTGTGCACTTACACTCAACACCTGATTG<br /> |rRNA<br /> |-<br /> |GACTTTCGTCCCTGCTCGACCCGTCGGTCTCACAGTCAAG<br /> |rRNA<br /> |-<br /> |TACCTTTTATCCGTTGAGCGACGGCGCTTCCACAAGCCACCGCCGGATCACTAGTC<br /> |rRNA<br /> |-<br /> |GTCGATATGGACTCTTGGGGAAGATCAGCCTGTTATCCCCGGGGTACCTTTTAT<br /> |rRNA<br /> |-<br /> |GACATCGAGGTGCCAAACCATCCCGTCGATATGGACTCTTG<br /> |rRNA<br /> |-<br /> |TTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTCCAGCCCCAGGATGCGACGA<br /> |rRNA<br /> |-<br /> |TCACGACGTTCTAAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACC<br /> |rRNA<br /> |-<br /> |GATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTC<br /> |rRNA<br /> |-<br /> |GTCCTCTCGTACTAGGGACAGCCCTTCTCAAGACTCCTAC<br /> |rRNA<br /> |-<br /> |TCTCACACGTATACGGTTTGGCCTCATCCGGTTTCGCTCGCCACTACTCCCGGAAT<br /> |rRNA<br /> |-<br /> |CATGCCCTTGGCAGGACAACTGGCACACCAGAGGTTCGTC<br /> |rRNA<br /> |-<br /> |CTTAGATGCTTTCAGCGGTTATCCCTCCCGAACGTAGCCAAC<br /> |rRNA<br /> |-<br /> |GTGGGAGTCCTCATCTCGAAGCAGGCTTCCCGCTTAGATG<br /> |rRNA<br /> |-<br /> |CTATCAACCCAGTCGTCTACTGGGAGCCTTAACCCCTCAAGG<br /> |rRNA<br /> |-<br /> |GCCTATTAGTACCGGTCACCTCCACACCTCACGGTGCTTCCAGATCCGGCCTATCAAC<br /> |rRNA<br /> |-<br /> |AGGACAAGCCCTCGGCCTATTAGTACCGGTCACCTCCACA<br /> |rRNA<br /> |-<br /> |CACACGCAACCCCTGCCGGGTCTCACACGTATACGGTTTG<br /> |rRNA<br /> |-<br /> |TTTCTGACTCGTCTCACGGCCGGCAGACCGTAAAAGAGAGATCCCACAACCCCGCACA<br /> |rRNA<br /> |-<br /> |TTTCGCATGTCCTTCGCCTACATCAACGGTTTCTGACTCGTCTCACGGCCGGCAGA<br /> |rRNA<br /> |-<br /> |CTTACCCTCTACGCCGGACCTTTCGCATGTCCTTCGCCTACATC<br /> |rRNA<br /> |-<br /> |GTGTCTCTCAAACGAGCCGTTGACGTTTCGACTACGGGGGTCTTACCCTCTA<br /> |rRNA<br /> |-<br /> |ATCCACCGTGCGCCCTTAAAAACTTGGCCACAGATGCTCG<br /> |rRNA<br /> |-<br /> |GATTTCTCGGGCCCCGTGCTACTTGGGTGTCTCTCAAACGAG<br /> |rRNA<br /> |-<br /> |GAATATTTAGGCTTAGCGGGTGGTCCCGCCAGATTCACACGGGATTTCTC<br /> |rRNA<br /> |-<br /> |CTCACGGTACTATCCGCTATCGGTCACCAGGGAATATTTAGGCTTAG<br /> |rRNA<br /> |-<br /> |GTTTCAGGTACTATTTCACTCCGCTCCCGCGGTACTTTTCACCATTC<br /> |rRNA<br /> |-<br /> |GATGGAGCAAGCTCCATCCCGACGCTCCCACGGCTTGTAGGCACACGGTTTCAGGTACT<br /> |rRNA<br /> |-<br /> |CACGCAGTCACGAGGATGGAGCAAGCTCCATCCCGACGCTC<br /> |rRNA<br /> |-<br /> |CTCGGGTTAACCTCGCAACACACCGCAAACTCGCAGGCTCATTCTTCAAAAGGCACGC<br /> |rRNA<br /> |-<br /> |CTACGGCTTCCCCACTCGGGTTAACCTCGCAACACACCGC<br /> |rRNA<br /> |-<br /> |GTCTTGAGCGTGCTACTAAAAACGCCCTATTCGGACTCGC<br /> |rRNA<br /> |-<br /> |CTCCCACGTCCTTCATCGGTTCCTGGTGCCAAGGCATCCAC<br /> |rRNA<br /> |-<br /> |CTTCAACCTGCCCATGGCTAGATCACTCCGCTTCGGGTCTTGAG<br /> |rRNA<br /> |-<br /> |GGTTCGGTCCTCCACGACCTCTTACAGCCGCTTCAACCTG<br /> |rRNA<br /> |-<br /> |CTAACCACAGGTCATCCCCCAGGTTTTCAACCCTGGTGGGTTCGGTCC<br /> |rRNA<br /> |-<br /> |AGAACCAGCTATCACGGAGTTTGATTGGCCTTTCACCCCTAACCACAGGTCATC<br /> |rRNA<br /> |-<br /> |CAAGAAACACACGACGCTGCACCTAAATGCATTTCGGGGAGAACCAGCTATCA<br /> |rRNA<br /> |-<br /> |TTTTAGCCTTGCGGCCGTACTCCCCAGGCGGGGCACTTAATGCGTTAGCTGCGGCAC<br /> |rRNA<br /> |-<br /> |CCGTCAATTCCTTTGAGTTTTAGCCTTGCGGCCGTACTCC<br /> |rRNA<br /> |-<br /> |AATAATCCGACCGTTCACAGCGTCCTCGCTGTTGTGTTACTTCAAAGGAA<br /> |rRNA<br /> |-<br /> |TTCTTCGCGTTGCGTCGAATTAAGCCACATGCTCCGCCGCTTGTGCGGGCCCCCGTCAA<br /> |rRNA<br /> |-<br /> |GTGTATGTCAAGCCTTGGTAAGGTTCTTCGCGTTGCGTCGAATTAAG<br /> |rRNA<br /> |-<br /> |GGGGGCACCATCTCTGATGCTTTCCGGTGTATGTCAAGCC<br /> |rRNA<br /> |-<br /> |TCACGACACGAGCTGACGACAGCCATGCACCACCTGTACACCGACCACAAGGGGGGCACC<br /> |rRNA<br /> |-<br /> |CTTAACCCAACATCTCACGACACGAGCTGACGACAGCCATG<br /> |rRNA<br /> |-<br /> |CAACACGGGACAAGGGTTGCGCTCGTTGCGGGACTTAACC<br /> |rRNA<br /> |-<br /> |GTCTCCCGTGAGTCCCCAACACCCCGAAGGGCTTGCTGGCAAC<br /> |rRNA<br /> |-<br /> |ACATAAGGGGCATGATGACTTGACGTCGTCCCCACCTTCCTCCGAGTTGACCCCG<br /> |rRNA<br /> |-<br /> |ATTGTACCGGCCATTGTAGCACGTGTGCAGCCCAAGACATAAGGGGCATGATGACTTGA<br /> |rRNA<br /> |-<br /> |TTTTGAGATTCGCTCCACCTTGCGGTATCGCAGCTCATTGTACCGGCCATTGTAGCACGT<br /> |rRNA<br /> |-<br /> |CAATCCGAACTGAGACCGGCTTTTTGAGATTCGCTCCACCTTGCGGTAT<br /> |rRNA<br /> |-<br /> |ATTACTAGCGACTCCGACTTCATGGGGTCGAGTTGCAGACCCCAATCCGAACTGAGA<br /> |rRNA<br /> |-<br /> |TATTCACCGCAGCAATGCTGATCTGCGATTACTAGCGACTCCGACTTCATG<br /> |rRNA<br /> |-<br /> |GTGTACAAGGCCCGGGAACGTATTCACCGCAGCAATGCTG<br /> |rRNA<br /> |-<br /> |GTAATCGGGTGACACCACGAGAGCGGAACAGTCGGAGGAATAA<br /> |rRNA<br /> |-<br /> |GTGTTACCGACTTTCGTGACGTGACGGGCGGTGTGTACAAG<br /> |rRNA<br /> |-<br /> |CACCTTCGACAGCTCCCTCCCACAAGGGGTTGGGCCACCGGCTTCGGGTGTTA<br /> |rRNA<br /> |-<br /> |CTACCTTGTTACGACTTCGTCCCAATCGCCAGTCCCACCTTCGACAG<br /> |rRNA<br /> |-<br /> |TCCTTAGAAAGGAGGTGATCCAGCCGCACCTTCCGGTACGGCTACCTTGTTACGA<br /> |rRNA<br /> |-<br /> |TGCAGTCGGCTATGTGCTCCTTAGAAAGGAGGTGATCCAG<br /> |rRNA<br /> |-<br /> |GATCAAACTCTCCGTGAATGTATACCCGTAATCGGGTGAC<br /> |rRNA<br /> |-<br /> |AAGTGGTTCATCGTTCGACTTGCATGTGTTAAGCACGCCGCCAGCGTTCGTCCTGA<br /> |rRNA<br /> |-<br /> |TATCAACATATCTGGCGTTGACTTTTGGCACGCTGTTGAGTTCTCAAGGAA<br /> |rRNA<br /> |-<br /> |TGTTACTCACCCGTTCGCCACTAATCCCCACCGAAGTGGTTCATCGTTCGACTT<br /> |rRNA<br /> |-<br /> |CTTGTCCCAGAGTGAAGGGCAGATTGCCCACGTGTTACTC<br /> |rRNA<br /> |-<br /> |GCAGATGCCTGCGAGGGTCAGTATCCGGTATTAGACCCCGTTTCCAGGGCTTGTCCCA<br /> |rRNA<br /> |-<br /> |ATCCTTCACCGCCGGAGCTTTCGAACCTCGCAGATGCCTGCGAGGGTCAGTATCC<br /> |rRNA<br /> |-<br /> |GATAGGCCGCGGGCTCATCCTTCACCGCCGGAGCTTTCGA<br /> |rRNA<br /> |-<br /> |GTCGCCTTGGTGAGCCATTACCTCACCAACAAGCTGATAG<br /> |rRNA<br /> |-<br /> |TAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGGCCGGTCGCCCTCTCAGGCCGGCTA<br /> |rRNA<br /> |-<br /> |ATTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTC<br /> |rRNA<br /> |-<br /> |CGTCGCTGCATCAGGCTTTCGCCCATTGTGCAATATTCCC<br /> |rRNA<br /> |-<br /> |CTTCTTCCCTGCTGAAAGAGGTTTACAACCCGAAGGCCGTCATC<br /> |rRNA<br /> |-<br /> |TTCAAAGGAACCTCAACCCGAACGGAGATCCGATCAGGTCGGGGTATCAACATAT<br /> |rRNA<br /> |-<br /> |GTAGTTAGCCGGCGCTTCTTCTGCAGGTACCGTCACTTTC<br /> |rRNA<br /> |-<br /> |ATAATTCCGGACAACGCTTGCGCCCTACGTATTACCGCGGCTGCTGGCACGTAGTTA<br /> |rRNA<br /> |-<br /> |CTTTCACAACCGACGTGACAAGCCGCCTACGAGCTCTTTACGCCCAATAATT<br /> |rRNA<br /> |-<br /> |TATCGACTGCAGACCCGGGGTTAAGCCCCGGGCTTTCACAACCGACGTGACAAG<br /> |rRNA<br /> |-<br /> |GATCTCCCCTACCGAACTCTAGCCTGCCCGTATCGACTGCAGAC<br /> |rRNA<br /> |-<br /> |CTGATATCTGCGCATTTCACCGCTACACCAGGAATTCCGATCTC<br /> |rRNA<br /> |-<br /> |CAGAGATCCGCCTTCGCCACCGGTGTTCCTCCTGATATCTG<br /> |rRNA<br /> |-<br /> |CACGCTTTCGCTCCTCAGCGTCAGTATCGGCCCAGAGATC<br /> |rRNA<br /> |-<br /> |CACCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTTC<br /> |rRNA<br /> |-<br /> |ACTTAATGCGTTAGCTGCGGCACGGACAACGTGGAATGTTGCCCACACCTAGTGCCCA<br /> |rRNA<br /> |-<br /> |GTTCGAAATGTAACCGGGCGTTTCCCCTACGCTATGACCACCGAAA<br /> |rRNA<br /> |-<br /> |CTTAGGATGGTTATAGTTACCACCGCCGTTTACTGGCGCTTAAGTTCTCA<br /> |rRNA<br /> |-<br /> |CCAAGCAAGCTTGATGTCCGACGCTCCCACGGCTTGTAGGCACACGGTTTCAGGTACTA<br /> |rRNA<br /> |-<br /> |CAGTCACGAGACACCAAGCAAGCTTGATGTCCGACGCTCC<br /> |rRNA<br /> |-<br /> |CTTGAGCGTGCTACTAAAAGCGCCCTATTCGGACTCGCTT<br /> |rRNA<br /> |-<br /> |CTACCTTGTTACGACTTCGTCCCAATCGCCAGTCCCACCTTCGACAGCTCCCT<br /> |rRNA<br /> |-<br /> |AAGGAGGTGATCCAGCCGCACCTTCCGGTACGGCTACCTTGTTACGACTTCGTC<br /> |rRNA<br /> |-<br /> |CCAGGATCAAACTCTCCGTGAATGTATACCCGTAATCGGG<br /> |rRNA<br /> |-<br /> |CTACCTTGTTACGACTTCGTCCCAATCGCCAGTCCCACCTTCG<br /> |rRNA<br /> |-<br /> |CTCTCACTTACCGGCATTCGGAGTTTGGCTAAGGTCAGTAAC<br /> |rRNA<br /> |-<br /> |CATTCGGACACCCCCGGATCAAAGCCTGGTTGACGACTCC<br /> |rRNA<br /> |-<br /> |GCTTATCCCCCACAGTCTCACTGCCGCGCTCTCACTTACC<br /> |rRNA<br /> |-<br /> |CTTAGTCGATGCTCTGGGCTGTTTCCCTCTCGACCATGGAGCTTATC<br /> |rRNA<br /> |-<br /> |CACATCCTTTCCCACTTAGCGTACGCTTAGGGGCCTTAGTCGATGCTCTG<br /> |rRNA<br /> |-<br /> |CTTCTAAGCCAACCTCCTGGTTGTCTGTGCGACTCCACATCCTTTC<br /> |rRNA<br /> |-<br /> |AATCACTAGACCAGTGAGCTATTACGCACTCTTTCAAGGGTGGCTGCTTCTAAGC<br /> |rRNA<br /> |-<br /> |CTACATTGTCGGCGCGGAATCACTAGACCAGTGAGCTATTA<br /> |rRNA<br /> |-<br /> |CGTATTGCTGCAATGACACGACTTCGGCGGTACGCTTGAG<br /> |rRNA<br /> |-<br /> |GCTCCCCTACCCATCACAGCCGGCGTTGGCCGTATTGCTG<br /> |rRNA<br /> |-<br /> |TTCTCACTCGTGAACCGTCCACAACTCGCTTCCGCGGCTGCTTCACCCGGCACACGACG<br /> |rRNA<br /> |-<br /> |GTTTGTATCGCTACTCATGCCTGCATTCTCACTCGTGAACCGTCCACAAC<br /> |rRNA<br /> |-<br /> |CTTAGTCAATCGGCGCAAACGTTTCTCACGTTTGTATCGCTACTCATG<br /> |rRNA<br /> |-<br /> |GACTTACCCTGGGCAGATCAGCTTGACCCAGGAACCCTTAGTCAATC<br /> |rRNA<br /> |-<br /> |TCCACACTGCCTATGTGTTCAGCAGCGGGTGACAGCCCATGACGACTGCCGGGTTT<br /> |rRNA<br /> |-<br /> |TTATCCATCGACTACGCCTGTCGGCCTCGCCTTAGGTCCCGACTTACCCTGGGCA<br /> |rRNA<br /> |-<br /> |GTACCGGAATATCAACCGGTTATCCATCGACTACGCCTGTC<br /> |rRNA<br /> |-<br /> |GGCCTTAGCATCACGATGCTCGATGTTTGACGCTTCACAG<br /> |rRNA<br /> |-<br /> |CTACTAACCGCTTGGTTCGGCGGCTCCACCACTCCCCTCAACTCCGAAGAGATCA<br /> |rRNA<br /> |-<br /> |GATGGACTACCTTCCTGCGTCACCCCATCACTCACCTACTAAC<br /> |rRNA<br /> |-<br /> |CCGGCCTACACCCTTACCCCGGGACAACCACCGCCCGGGATGGACTACC<br /> |rRNA<br /> |-<br /> |CAAGGCATCCACCGTGCGCCCTTAAAAACTTGGCCACAGA<br /> |rRNA<br /> |-<br /> |CAAGGTGTGCGGATTTACCTACACACCGGCCTACACCCTTAC<br /> |rRNA<br /> |-<br /> |CCACAATCGGCTCGGCATCAGGTCTCAGCCACAAGGTGTG<br /> |rRNA<br /> |-<br /> |CTAGAGGCTTTTCTCGACAGCATAGGATCATCCACTTCACCACAATC<br /> |rRNA<br /> |-<br /> |TTTAGGGTACGGGCCGCCATGAAACTCGCTAGAGGCTTTTCTC<br /> |rRNA<br /> |-<br /> |CTCGGTATTCTCTACCTGACCACCTGAGTCGGTTTAGGGTAC<br /> |rRNA<br /> |-<br /> |GGCATTTTGCCGAGTTCCTTAACCATAGTTCACCCGAACG<br /> |rRNA<br /> |-<br /> |CAGCTCAGAGTGCAAAACTCGTCACCAGGTGTGGCCCCCCTTCTCCCGAAGTTA<br /> |rRNA<br /> |-<br /> |TTGTTTTCTCTTCCTGCGGGTACTGAGATGTTTCACTTCCCCGCGTTCCCTCCACA<br /> |rRNA<br /> |-<br /> |TGTGTTTTTAGTAAACAGTCGCTTCTCGCTGGTCTCTGCGGCCACCCCCAGCTCAGA<br /> |rRNA<br /> |-<br /> |TATACATCGCCTTACGGCTTCGCACGGACCTGTGTTTTTAGTAAACAGTCGCTTCT<br /> |rRNA<br /> |-<br /> |GCAGGCGTCAGTCCGTATACATCGCCTTACGGCTTCGCAC<br /> |rRNA<br /> |-<br /> |GAAATGTGACTAACCGGTCCCCTTAACGTTCCAGCACCGGGCAGGC<br /> |rRNA<br /> |-<br /> |GTTTACTGGCGCTTAAGTTCTCAGCTTCGCCACACCGAAATGTGACTAAC<br /> |rRNA<br /> |-<br /> |GACAAGGAATTTCGCTACCTTAGGATGGTTATAGTTACCACCGCCGTTTA<br /> |rRNA<br /> |-<br /> |AAGTCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTA<br /> |rRNA<br /> |-<br /> |CTATGGTTGAGACAGTCGAGAAGTCGTTACGCCATTCGTG<br /> |rRNA<br /> |-<br /> |CTGCGCGAAACGAGCATCTTTACTCGTAGTGCAATTTCAC<br /> |rRNA<br /> |-<br /> |CAATATCAAACTGTAGTAAAGGTCCCGGGGTCTTTCCGTCCTGCTGCGCGAAA<br /> |rRNA<br /> |-<br /> |CAGCTATCCTACACAAGCCGAACCGAACACCAATATCAAACTGTAGTAAA<br /> |rRNA<br /> |-<br /> |CACACTGGCGTGCGAGCTTCAAAGTCTCCCAGCTATCCTACACAAG<br /> |rRNA<br /> |-<br /> |TTAGACATCCAGCACGACCAGACTGGTATTTCAACGACGACTCCACCCACACTGGCGTG<br /> |rRNA<br /> |-<br /> |GTTTCGCTCGCCACTACTCCCGGAATCACGGTTGTTTTCTCTTC<br /> |rRNA<br /> |-<br /> |GATCACGGACCCAGGTTAGACATCCAGCACGACCAGACTG<br /> |rRNA<br /> |-<br /> |CAGTTAAACTACCCATCAGACACTGTCCCTGATCCGGATCAC<br /> |rRNA<br /> |-<br /> |GAACCTTTGGGCGCCTCCGTTACTCTTTAGGAGGCAACCG<br /> |rRNA<br /> |-<br /> |CTTGTGCACTTACACTCAACACCTGATTGCCAACCAGGCTGAGGGAACCTTT<br /> |rRNA<br /> |-<br /> |GACCCGTCGGTCTCACAGTCAAGCTCCCTTGTGCACTTAC<br /> |rRNA<br /> |-<br /> |CTTCCACAAGCCACCGCCGGATCACTAGTCCCGACTTTCGTCCCTG<br /> |rRNA<br /> |-<br /> |GTACCTTTTATCCGTTGAGCGACGGCGCTTCCACAAGCCAC<br /> |rRNA<br /> |-<br /> |CAAACCATCCCGTCGATATGGACTCTTGGGGAAGATCAGCCTGTTATC<br /> |rRNA<br /> |-<br /> |CCAGGATGCGACGAGCCGACATCGAGGTGCCAAACCATCC<br /> |rRNA<br /> |-<br /> |GTCTCACACGTATACGGTTTGGCCTCATCCGGTTTCGCTC<br /> |rRNA<br /> |-<br /> |CTTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTCCAG<br /> |rRNA<br /> |-<br /> |GAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCGCTTTAATG<br /> |rRNA<br /> |-<br /> |CTTCTCAAGACTCCTACGCGCACAGCGGATAGGGACCGAAC<br /> |rRNA<br /> |-<br /> |CACACCAGAGGTTCGTCCGTCCCGGTCCTCTCGTACTAGG<br /> |rRNA<br /> |-<br /> |TTAGATGCTTTCAGCGGTTATCCCTCCCGAACGTAGCCAACCAGCCATGCCCTTGGCA<br /> |rRNA<br /> |-<br /> |CTCATCTCGAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATC<br /> |rRNA<br /> |-<br /> |CAGTCGTCTACTGGGAGCCTTAACCCCTCAAGGGGGTGGGAGTCCTCATCTCGAA<br /> |rRNA<br /> |-<br /> |CTTCCAGATCCGGCCTATCAACCCAGTCGTCTACTGGGAG<br /> |rRNA<br /> |-<br /> |CAGACCGTAAAAGAGAGATCCCACAACCCCGCACACGCAACCCCTGCCGGGTCTCA<br /> |rRNA<br /> |-<br /> |TACATCAACGGTTTCTGACTCGTCTCACGGCCGGCAGACCGTAAAAGAGAGAT<br /> |rRNA<br /> |-<br /> |CTTTCGCATGTCCTTCGCCTACATCAACGGTTTCTGACTC<br /> |rRNA<br /> |-<br /> |ACTACGGGGGTCTTACCCTCTACGCCGGACCTTTCGCATG<br /> |rRNA<br /> |-<br /> |CTACTTGGGTGTCTCTCAAACGAGCCGTTGACGTTTCGACTAC<br /> |rRNA<br /> |-<br /> |GCCAGATTCACACGGGATTTCTCGGGCCCCGTGCTACTTG<br /> |rRNA<br /> |-<br /> |TATCCGCTATCGGTCACCAGGGAATATTTAGGCTTAGCGGGTGGTCCCGCCAGATT<br /> |rRNA<br /> |-<br /> |GTACTTTTCACCATTCCCTCACGGTACTATCCGCTATCGGTCAC<br /> |rRNA<br /> |-<br /> |CTTGTAGGCACACGGTTTCAGGTACTATTTCACTCCGCTCCC<br /> |rRNA<br /> |-<br /> |GGGCCTATCGTGGCCTCCCACGTCCTTCATCGGTTCCTGG<br /> |rRNA<br /> |-<br /> |CACGCAGTCACGAGGATGGAGCAAGCTCCATCCCGACGCTCCCACGGCTTGTAGGCA<br /> |rRNA<br /> |-<br /> |CATTCTTCAAAAGGCACGCAGTCACGAGGATGGAGCAAGC<br /> |rRNA<br /> |-<br /> |TACGGCTTCCCCACTCGGGTTAACCTCGCAACACACCGCAAACTCGCAGGCTCAT<br /> |rRNA<br /> |-<br /> |TCGGACTCGCTTTCGCTACGGCTTCCCCACTCGGGTTAAC<br /> |rRNA<br /> |-<br /> |ATCACTCCGCTTCGGGTCTTGAGCGTGCTACTAAAAACGC<br /> |rRNA<br /> |-<br /> |CTCTTACAGCCGCTTCAACCTGCCCATGGCTAGATCACTC<br /> |rRNA<br /> |-<br /> |GTTTTCAACCCTGGTGGGTTCGGTCCTCCACGACCTCTTAC<br /> |rRNA<br /> |-<br /> |GTTTGATTGGCCTTTCACCCCTAACCACAGGTCATCCCCCAGGTTTTCAACC<br /> |rRNA<br /> |-<br /> |GGAGAACCAGCTATCACGGAGTTTGATTGGCCTTTCACCC<br /> |rRNA<br /> |-<br /> |TATCCAGTGCTCTACCTCCGGCAAGAAACACACGACGCTGCACCTAAATGCATTT<br /> |rRNA<br /> |-<br /> |GAGTTTGGCTAAGGTCAGTAACCCGGTAGGGCCCATCGCCTATCCAGTGCTCTA<br /> |rRNA<br /> |-<br /> |CTGTAAGGCTTAGCTTCCGGGTTCGAAATGTAACCGGGCGTTTCCCCTAC<br /> |rRNA<br /> |-<br /> |GTTCGGCGGCGTCCTACTCTCCCACAGGGTCCCCCCTGCAGTACCATCGGCGCTGTA<br /> |rRNA<br /> |-<br /> |GTATTAGACCCCGTTTCCAGGGCTTGTCCCAGAGTGAAGGGCAGATT<br /> |rRNA<br /> |-<br /> |GAGCTTTCGAACCTCGCAGATGCCTGCGAGGGTCAGTATCCGGTATTAGACCCCGTT<br /> |rRNA<br /> |-<br /> |CATCCTTCACCGCCGGAGCTTTCGAACCTCGCAGATGCCT<br /> |rRNA<br /> |-<br /> |CACCGAAGTGGTTCATCGTTCGACTTGCATGTGTTAAGCAC<br /> |rRNA<br /> |-<br /> |TTCGACTTGCATGTGTTAAGCACGCCGCCAGCGTTCGTCCTGAGCCAGGATCAAACTCT<br /> |rRNA<br /> |-<br /> |CAGATTGCCCACGTGTTACTCACCCGTTCGCCACTAATCC<br /> |rRNA<br /> |-<br /> |GCGGATTTACCTACCTGACGTCCTACACCCTTACCCCGGGACAACCACCGC<br /> |rRNA<br /> |-<br /> |CATCAGGTCTCAGACTCATGTCAGGCGGATTTACCTACCTGAC<br /> |rRNA<br /> |-<br /> |CTATTAGTACCGGTCACCTCCACACCTCACGGTGCTTCCAGATCCGGCCTATCAACC<br /> |rRNA<br /> |-<br /> |GGACAAGCCCTCGGCCTATTAGTACCGGTCACCTCCACAC<br /> |rRNA<br /> |-<br /> |CCGGGTTAACCTCGCAACACACCGCAAACTCGCAGGCTCATTCTTCAAAAGGCACGC<br /> |rRNA<br /> |-<br /> |GGTCTTGAGCGTGCTACTAAAACGCCCTATTCGGACTCGC<br /> |rRNA<br /> |-<br /> |TTCGGCGGCGTCCTACTCTCCCACAGGGTCCCCCCTGCAGTACCATCGGCGCTGTAA<br /> |rRNA<br /> |-<br /> |CCGAAGCCTCCGGCGGTCTGCTTTCTGTGGCACTGTCCCG<br /> |SCOs01<br /> |-<br /> |CTGGGCACTGGTGGTCTCTTACACCACCGTTTCACCCTTAC<br /> |SCOs01<br /> |-<br /> |TTCCTTCTTGACCTTGCTCCGGGTGGGGTTTACCTAGCTGCCCAGGTCGCCCTG<br /> |SCOs01<br /> |-<br /> |CTCGAACGTCCGCGCAGAAGCACCGGGGTGCTTCCTTCTTGACCTTGCTC<br /> |SCOs01<br /> |-<br /> |CTCCAGCGGTCTACCCGCGGACTCGGGCGGGCAGCCCTCGAACGTCCGCGCAGAAG<br /> |SCOs01<br /> |-<br /> |CATCCATCTAGGACCGGCGTTGCCACCGGCCTCCAGCGGTCTAC<br /> |SCOs01<br /> |-<br /> |CTGTGGAGCCCGGACGTTCCTCGGGAAGTCCCGTGAGGGACTC<br /> |SCOs01<br /> |-<br /> |GTTCTGTTCCGACGGGGCCTCGCGGTCCCGTCGGCGACGGCCATCCAT<br /> |SCOs01<br /> |-<br /> |CAGACGAGTCGGGCTGTACGCCGGGTTCTGTTCCGACGGG<br /> |SCOs01<br /> |-<br /> |GTCTGCTTTCTGTGGCACTGTCCCGCGGGTCACCCCGGGTGGCCGTTAGCCATCAC<br /> |SCOs01<br /> |-<br /> |CTTAGTAGGAGGCTCGCTTCGCTACCTGAGATCAGGCAGCGAGGGCGAAGG<br /> |SCOs02<br /> |-<br /> |GAACAGCCCCGGGCTGACACTCCCTTAGTAGGAGGCTCGCTTC<br /> |SCOs02<br /> |-<br /> |GATCAAGGTTTAGTCCCCTAGCTGATGCCAGGATCCGGGTC<br /> |SCOs02<br /> |-<br /> |CAGTCACTGTTTGGTTTCCCTCTTCACCCCGTGACCGGGATCAA<br /> |SCOs02<br /> |-<br /> |GAGATCACGCGGACAAGTCTCCGACGGGCCCAGTCACTGT<br /> |SCOs02<br /> |-<br /> |CTTCCCCTGCTTCGACAGCCGCTGTCGAAACCGATCATCC<br /> |SCOs02<br /> |-<br /> |AATCAGGGCTTCTCCGTGTGCAGTCCGCTTCGATTTTCTCGGCCCCGGAGATCACG<br /> |SCOs02<br /> |-<br /> |GAATCGAACCCGCGTCCAACGGTGCGGAATCAGGGCTTCTC<br /> |SCOs02<br /> |-<br /> |TTTTTCGGCCTGTGGTTTACGAGATCATGGCCGCTTCCTC<br /> |SCOs02<br /> |-<br /> |CTGCTGGGAGGAATCGCGCTTGGTGTTGGCGATTATTTTT<br /> |SCOs02<br /> |-<br /> |CACGCACCCGCCAGAGCCGACCTACCCTTGCTGCCTTCCG<br /> |SCOs03<br /> |}</div> RTEvans https://actinobase.org/index.php?title=RNA_Sequencing&diff=2194 RNA Sequencing 2023-11-01T16:48:35Z <p>RTEvans: </p> <hr /> <div>==Instructions==<br /> <br /> This procedure is an example and the optimal library preparation and data analysis procedures can be different depending on the type of the DNA sequencer used and purpose of the experiment.<br /> <br /> #Extract and purify RNA samples according to [[RNA extraction from Actinobacteria]]. Add [https://www.thermofisher.com/order/catalog/product/4456740#/4456740 ERCC RNA Spike-In Mix] if necessary.<br /> #Check the quality of RNA samples using [https://www.chem.agilent.com/store/productDetail.jsp?catalogId=5067-1511&amp;catId=SubCat2ECS_227716 Agilent RNA 6000 Nano Kit] or by running them on an agarose gel.<br /> #Deplete rRNA using [https://www.illumina.com/products/by-type/accessory-products/ribo-zero-plus-rrna-depletion.html Illumina Ribo-Zero Plus rRNA Depletion Kit] or a similar product, or using the [[rRNA depletion with Streptomyces coelicolor A3(2) specific probes]] protocol.<br /> #Create Illumina RNA-seq libraries using [https://www.illumina.com/products/by-type/sequencing-kits/library-prep-kits/truseq-rna-v2.html TruSeq RNA Library Prep Kit].<br /> #Sequence the libraries using an Illumina system such as [https://www.illumina.com/systems/sequencing-platforms/novaseq.html NovaSeq 6000 Sequencing System].<br /> #Trim low quality subsequences from reads using [http://hannonlab.cshl.edu/fastx_toolkit/ FASTX-Toolkit] (single-end) or [https://research.bioinformatics.udel.edu/genomics/ngsShoRT/ ngsShoRT] (single- or paired-end).<br /> #Align reads with the reference genome using [http://daehwankimlab.github.io/hisat2/ HISAT2] or [http://bowtie-bio.sourceforge.net/bowtie2/index.shtml Bowtie2].<br /> #Convert the SAM files into BAM files and sort and index the BAM files using [http://www.htslib.org/ Samtools].<br /> #Count the number of reads overlapping with each feature such as CDS or tRNA gene using [http://subread.sourceforge.net/ featureCounts] or [https://htseq.readthedocs.io/en/master/ HTSeq].<br /> #Perform a differential gene expression analysis using a Bioconductor package such as [https://bioconductor.org/packages/release/bioc/html/DESeq2.html DESeq2].</div> RTEvans https://actinobase.org/index.php?title=RRNA_depletion_with_Streptomyces_coelicolor_A3(2)_specific_probes.&diff=2193 RRNA depletion with Streptomyces coelicolor A3(2) specific probes. 2023-11-01T16:44:44Z <p>RTEvans: RTEvans moved page RRNA depletion with Streptomyces coelicolor A3(2) specific probes. to RRNA depletion with Streptomyces coelicolor A3(2) specific probes over redirect</p> <hr /> <div>#REDIRECT [[RRNA depletion with Streptomyces coelicolor A3(2) specific probes]]</div> RTEvans https://actinobase.org/index.php?title=RRNA_depletion_with_Streptomyces_coelicolor_A3(2)_specific_probes&diff=2192 RRNA depletion with Streptomyces coelicolor A3(2) specific probes 2023-11-01T16:44:44Z <p>RTEvans: RTEvans moved page RRNA depletion with Streptomyces coelicolor A3(2) specific probes. to RRNA depletion with Streptomyces coelicolor A3(2) specific probes over redirect</p> <hr /> <div>==Organisms==<br /> <br /> This protocol has been tested with &lt;em&gt;[[Streptomyces coelicolor]]&lt;/em&gt; A3(2) MT1110<br /> <br /> ==Description==<br /> <br /> rRNA depletion using commercial kits targeting generic bacterial sequences is not 100% efficient in the High GC organism S. coelicolor. <br /> <br /> Depletion using the NEBNext RNA Depletion Core Reagent Set with RNA Sample Purification Beads (https://www.neb.com/en-gb/products/e7870-nebnext-rna-depletion-core-reagent-set-with-rna-sample-purification-beads#Product%20Information) and custom DNA probes designed for &lt;em&gt;S. coelicolor&lt;/em&gt; is more effective than the QIAseq FastSelect 5S/16S/23S kit (Qiagen #335927); NEBNext rRNA Depletion Kit (Bacteria) (NEB #E7850); Illumina Ribo-zero rRNA Removal Kit (Bacteria) (Illumina #MRZMB12424, discontinued). The Illumina kit tested was subsequently replaced by the Illumina Ribo-Zero Plus rRNA Depletion Kit (Illumina #20040526). Additionally three abundant non-mRNA RNAs can be depleted (Table 1).<br /> <br /> '''Table 1.''' Abundant non-mRNA RNAs in &lt;em&gt;S. coelicolor&lt;/em&gt;.<br /> <br /> [[File: rRNA_depletion_table_1.png]]<br /> <br /> ==Materials==<br /> <br /> Single stranded DNA probes (Table 2) at 2µM concentration in IDTE pH 7.5 (10mM Tris-HCl / 0.1mM EDTA). Integrated DNA Technologies can produce these from their 25nmole DNA oligo pool product. Probes are listed in Table 2 in the Notes section below.<br /> <br /> NEBNext RNA Depletion Core Reagent Set with RNA Sample Purification Beads (https://www.neb.com/en-gb/products/e7870-nebnext-rna-depletion-core-reagent-set-with-rna-sample-purification-beads#Product%20Information).<br /> <br /> ==Protocols==<br /> <br /> As per manufacturer’s instructions.<br /> <br /> ==Notes==<br /> <br /> The following histograms illustrate effectiveness of rRNA depletion using various methods. Histograms show percentage of bases aligned to NCBI reference sequence NC_003888.3 using Hisat2 program classified as follows - Coding = RNA aligned to coding sequences; Transcribed not translated = RNA aligned to rRNA, tRNA and sRNA; Intergenic = RNA aligned to 5' or 3' UTRs or between annotated sequences.<br /> <br /> '''QIAseq FastSelect 5S/16S/23S kit'''<br /> <br /> [[File: PanelA.png]] <br /> <br /> '''NEBNext rRNA Depletion Kit (Bacteria)'''<br /> <br /> [[File: PanelB.png]]<br /> <br /> '''Illumina Ribo-zero rRNA Removal Kit (Bacteria)'''<br /> <br /> [[File: PanelC.png]] <br /> <br /> '''rRNA depletion with &lt;em&gt;S. coelicolor&lt;/em&gt; specific probes<br /> <br /> [[File: PanelD.png]]<br /> <br /> <br /> '''Table 2''' List of probes<br /> <br /> {| class=&quot;wikitable&quot;<br /> !Sequence<br /> !Target<br /> |-<br /> |CAAGTCCTTGCGCGACGCTCCCACGGCTTGTAGGCACACGGTTTCAGGTACTATTT<br /> |rRNA<br /> |-<br /> |CAGTCACGACGCAAGGACAAGTCCTTGCGCGACGCTCCCAC<br /> |rRNA<br /> |-<br /> |ACCTCGCAACACACCGCAAACTCGCAGGCTCATTCTTCAAAAGGCACGCAGTCACGACG<br /> |rRNA<br /> |-<br /> |CTACGGCTTCCCCACCCGGGTTAACCTCGCAACACACCGC<br /> |rRNA<br /> |-<br /> |TTAACCCAACATCTCACGACACGAGCTGACGACAGCCATGCACCACCTGTACACCGA<br /> |rRNA<br /> |-<br /> |CGCTCGTTGCGGGACTTAACCCAACATCTCACGACACGAG<br /> |rRNA<br /> |-<br /> |CAACACCCCGAAGGGCTTGCTGGCAACACGGGACAAGGGTTG<br /> |rRNA<br /> |-<br /> |CACCTTCCTCCGAGTTGACCCCGGCGGTCTCCCGTGAGTC<br /> |rRNA<br /> |-<br /> |GACATAAGGGGCATGATGACTTGACGTCGTCCCCACCTTC<br /> |rRNA<br /> |-<br /> |GTATCGCAGCTCATTGTACCGGCCATTGTAGCACGTGTGCAGCCCAAGACATAA<br /> |rRNA<br /> |-<br /> |TTTTGAGATTCGCTCCACCTTGCGGTATCGCAGCTCATTGTAC<br /> |rRNA<br /> |-<br /> |CAGACCCCAATCCGAACTGAGACCGGCTTTTTGAGATTCG<br /> |rRNA<br /> |-<br /> |AATGCTGATCTGCGATTACTAGCGACTCCGACTTCATGGGGTCGAGTTGCAGAC<br /> |rRNA<br /> |-<br /> |GAACGTATTCACCGCAGCAATGCTGATCTGCGATTACTAG<br /> |rRNA<br /> |-<br /> |TACCGACTTTCGTGACGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTC<br /> |rRNA<br /> |-<br /> |GCCACCGGCTTCGGGTGTTACCGACTTTCGTGACGTGACG<br /> |rRNA<br /> |-<br /> |TTCGTCCCAATCGCCAGTCCCACCTTCGACAGCTCCCTCCCACAAGGGGTTGGG<br /> |rRNA<br /> |-<br /> |CGCACCTTCCGGTACGGCTACCTTGTTACGACTTCGTCCCAATCGCCAGTCCCA<br /> |rRNA<br /> |-<br /> |TTCGACCCTCGCAGATGCCTGCGAGGGTCAGTATCCGGTATTAGACCCCGTTTCCAG<br /> |rRNA<br /> |-<br /> |GAAAGGAGGTGATCCAGCCGCACCTTCCGGTACGGCTACC<br /> |rRNA<br /> |-<br /> |TTCACCGCCGGAGCTTTCGACCCTCGCAGATGCCTGCGAG<br /> |rRNA<br /> |-<br /> |CATTACCTCACCAACAAGCTGATAGGCCGCGGGCTCATCCTTC<br /> |rRNA<br /> |-<br /> |CTCTCAGGCCGGCTACCCGTCGTCGCCTTGGTGAGCCATTAC<br /> |rRNA<br /> |-<br /> |GTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGGCCGGTCG<br /> |rRNA<br /> |-<br /> |TGCATCAGGCTTTCGCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCT<br /> |rRNA<br /> |-<br /> |TGCTGAAAGAGGTTTACAACCCGAAGGCCGTCATCCCTCACGCGGCGTCGCTGCATCA<br /> |rRNA<br /> |-<br /> |TTCTTCTGCAGGTACCGTCACTTTCGCTTCTTCCCTGCTGAAAGAGGTTTACAA<br /> |rRNA<br /> |-<br /> |CTACGTATTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTCTG<br /> |rRNA<br /> |-<br /> |CTCTTTACGCCCAATAATTCCGGACAACGCTTGCGCCCTACGTATTAC<br /> |rRNA<br /> |-<br /> |GGGCTTTCACAACCGACGTGACAAGCCGCCTACGAGCTCT<br /> |rRNA<br /> |-<br /> |CTACCGAACTCTAGCCTGCCCGTATCGACTGCAGACCCGGGGTTAAGCCCCG<br /> |rRNA<br /> |-<br /> |ATATCTGCGCATTTCACCGCTACACCAGGAATTCCGATCTCCCCTACCGAACTCTAG<br /> |rRNA<br /> |-<br /> |CGGTGTTCCTCCTGATATCTGCGCATTTCACCGCTACACC<br /> |rRNA<br /> |-<br /> |ACGCTTTCGCTCCTCAGCGTCAGTATCGGCCCAGAGATCCGCCTTCGCCACCGGTGT<br /> |rRNA<br /> |-<br /> |GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCG<br /> |rRNA<br /> |-<br /> |ACAACGTGGAATGTTGCCCACACCTAGTGCCCACCGTTTACGGCGTGGACTACCA<br /> |rRNA<br /> |-<br /> |CTTAATGCGTTAGCTGCGGCACGGACAACGTGGAATGTTG<br /> |rRNA<br /> |-<br /> |TCAATTCCTTTGAGTTTTAGCCTTGCGGCCGTACTCCCCAGGCGGGGCACTTAA<br /> |rRNA<br /> |-<br /> |ATTAAGCCACATGCTCCGCCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTTA<br /> |rRNA<br /> |-<br /> |TCAAGCCTTGGTAAGGTTCTTCGCGTTGCGTCGAATTAAGCCACATGCTCCGCCGCTT<br /> |rRNA<br /> |-<br /> |CATCTCTGATGCTTTCCGGTGTATGTCAAGCCTTGGTAAGGTTCTTC<br /> |rRNA<br /> |-<br /> |ACAGCCATGCACCACCTGTACACCGACCACAAGGGGGGCACCATCTCTGATGCTTTCC<br /> |rRNA<br /> |-<br /> |TGTAACCGGGCGTTTCCCCTACGCTATGACCACCGAAACC<br /> |rRNA<br /> |-<br /> |CAGTACCATCGGCGCTGTAAGGCTTAGCTTCCGGGTTCGAAATGTAAC<br /> |rRNA<br /> |-<br /> |GTTCGGCGGCGTCCTACTCTCCCACAGGGTCCCCCCTGCA<br /> |rRNA<br /> |-<br /> |CACTCCCCTCAACTCCGAAGAGATCAGGGCGGCTTCACGG<br /> |rRNA<br /> |-<br /> |CCATCACTCACCTACTAACCGCTTGGTTCGGCGGCTCCAC<br /> |rRNA<br /> |-<br /> |GTCCTTCATCGGTTCCTGGTGCCAAGGCATCCACCGTGCGCCCTTAAAAACTT<br /> |rRNA<br /> |-<br /> |CTTGTAGGCACACGGTTTCAGGTACTATTTCACTCCGCTCCCG<br /> |rRNA<br /> |-<br /> |CACGCAGTCACGAGACACCAAGCAAGCTTGATGTCCGACGCTCCCACGGCTTGTAGGCAC<br /> |rRNA<br /> |-<br /> |TCTTCAAAAGGCACGCAGTCACGAGACACCAAGCAAGCTT<br /> |rRNA<br /> |-<br /> |CACCCGGGTTAACCTCGCAACACACTGCAAACTCGCAGGC<br /> |rRNA<br /> |-<br /> |GCGTGCTACTAAAACGCCCTATTCGGACTCGCTTTCGCTACGGCTTCC<br /> |rRNA<br /> |-<br /> |GATCACTCCGCTTCGGGTCTTGAGCGTGCTACTAAAACGC<br /> |rRNA<br /> |-<br /> |TCGCAGATGCCTGCAAGGGTCAGTATCCGGTATTAGACCCCGTTT<br /> |rRNA<br /> |-<br /> |TCATCCTTCACCGCCGCAGCTTTCGAACCTCGCAGATGCC<br /> |rRNA<br /> |-<br /> |ATGTAACCGGGCGTTTCCCCTACGCTATGACCACCGAAAC<br /> |rRNA<br /> |-<br /> |CGTCCTACTCTCCCACAGGGTCCCCCCTGCAGTACCATCG<br /> |rRNA<br /> |-<br /> |CATCGCCTATCCAGTGCTCTACCTCCGGCAAGAAACACAC<br /> |rRNA<br /> |-<br /> |CATTCGGAGTTTGGCTAAGGTCAGTAACCCGGTAGGGCCCATCG<br /> |rRNA<br /> |-<br /> |CAGTCTCACTGCCGCGCTCTCACTTACCGGCATTCGGAGTTTG<br /> |rRNA<br /> |-<br /> |ATGCTCTGGGCTGTTTCCCTCTCGACCATGGAGCTTATCCCCCACAGTCTCACTG<br /> |rRNA<br /> |-<br /> |CTTAGGGGCCTTAGTCGATGCTCTGGGCTGTTTCCCTCTCG<br /> |rRNA<br /> |-<br /> |GTTGTCTGTGCGACTCCACATCCTTTCCCACTTAGCGTACGCTTAG<br /> |rRNA<br /> |-<br /> |TATTACGCACTCTTTCAAGGGTGGCTGCTTCTAAGCCAACCTCCTGGTTGTCTGT<br /> |rRNA<br /> |-<br /> |GATCAAAGCCTGGTTGACGACTCCCCGGGGCCTATCGTGG<br /> |rRNA<br /> |-<br /> |CGGAATCACTAGACCAGTGAGCTATTACGCACTCTTTCAAGG<br /> |rRNA<br /> |-<br /> |TATTGCTGCAATGACACGACTTCGGCGGTACGCTTGAGCCCCGCTACATTGTCG<br /> |rRNA<br /> |-<br /> |CATCACAGCCGGCGTTGGCCGTATTGCTGCAATGACACGACTTC<br /> |rRNA<br /> |-<br /> |TCCACAACTCGCTTCCGCGGCTGCTTCACCCGGCACACGACGCTCCCCTACCCATCACA<br /> |rRNA<br /> |-<br /> |TACTCATGCCTGCATTCTCACTCGTGAACCGTCCACAACTCGCTTCCGCGGCTGCTT<br /> |rRNA<br /> |-<br /> |CAAACGTTTCTCACGTTTGTATCGCTACTCATGCCTGCATTCTCACTC<br /> |rRNA<br /> |-<br /> |CAGGAACCCTTAGTCAATCGGCGCAAACGTTTCTCACGTTT<br /> |rRNA<br /> |-<br /> |CTTAGGTCCCGACTTACCCTGGGCAGATCAGCTTGACCCAG<br /> |rRNA<br /> |-<br /> |GAATATCAACCGGTTATCCATCGACTACGCCTGTCGGCCTCGCCTTAGGTC<br /> |rRNA<br /> |-<br /> |TTAGCATCACGATGCTCGATGTTTGACGCTTCACAGCGGGTACCGGAATATCAA<br /> |rRNA<br /> |-<br /> |GAAGAGATCAGGGCGGCTTCACGGCCTTAGCATCACGATG<br /> |rRNA<br /> |-<br /> |CATCACTCACCTACTAACCGCTTGGTTCGGCGGCTCCACC<br /> |rRNA<br /> |-<br /> |TGTGTTCAGCAGCGGGTGACAGCCCATGACGACTGCCGGGTTTCCCCATTCGGACACCC<br /> |rRNA<br /> |-<br /> |GACAACCACCGCCCGGGATGGACTACCTTCCTGCGTCACCCCAT<br /> |rRNA<br /> |-<br /> |CAGATGCTCGCGTCCACTGTGCAGTTCTCAAACAACGACC<br /> |rRNA<br /> |-<br /> |TGCGGATTTACCTACACACCGGCCTACACCCTTACCCCGGGACAACCACCG<br /> |rRNA<br /> |-<br /> |CATCAGGTCTCAGCCACAAGGTGTGCGGATTTACCTACAC<br /> |rRNA<br /> |-<br /> |TTTTCTCGACAGCATAGGATCATCCACTTCACCACAATCGGCTCGGCATCAGGTCT<br /> |rRNA<br /> |-<br /> |CCATGAAACTCGCTAGAGGCTTTTCTCGACAGCATAGGATCATC<br /> |rRNA<br /> |-<br /> |TATTCTCTACCTGACCACCTGAGTCGGTTTAGGGTACGGGCCGCCATGAAACT<br /> |rRNA<br /> |-<br /> |GAGTTCCTTAACCATAGTTCACCCGAACGCCTCGGTATTCTCTACCTGAC<br /> |rRNA<br /> |-<br /> |CTTCTCCCGAAGTTACGGGGGCATTTTGCCGAGTTCCTTAACCA<br /> |rRNA<br /> |-<br /> |CACCCCCAGCTCAGAGTGCAAAACTCGTCACCAGGTGTGG<br /> |rRNA<br /> |-<br /> |GTGTTTTTAGTAAACAGTCGCTTCTCGCTGGTCTCTGCGG<br /> |rRNA<br /> |-<br /> |GTTCCCTCCACACTGCCTATGTGTTCAGCAGCGGGTGACAG<br /> |rRNA<br /> |-<br /> |GTATACATCGCCTTACGGCTTCGCACGGACCTGTGTTTTTA<br /> |rRNA<br /> |-<br /> |CTTAACGTTCCAGCACCGGGCAGGCGTCAGTCCGTATACATC<br /> |rRNA<br /> |-<br /> |TAAGTTCTCAGCTTCGCCACACCGAAATGTGACTAACCGGTCCCCTTAACGTT<br /> |rRNA<br /> |-<br /> |TTAGGATGGTTATAGTTACCACCGCCGTTTACTGGCGCTTAAGTTCTCAG<br /> |rRNA<br /> |-<br /> |GTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGATGGTTATAGT<br /> |rRNA<br /> |-<br /> |TATGGTTGAGACAGTCGAGAAGTCGTTACGCCATTCGTGCAGGTCGGAACTTACC<br /> |rRNA<br /> |-<br /> |GAAACGAGCATCTTTACTCGTAGTGCAATTTCACCGGGCCTATGGTTGAG<br /> |rRNA<br /> |-<br /> |GGTCTTTCCGTCCTGCTGCGCGAAACGAGCATCTTTACTC<br /> |rRNA<br /> |-<br /> |GAACCGAACACCAATATCAAACTGTAGTAAAGGTCCCGGGGTCTTTC<br /> |rRNA<br /> |-<br /> |CTTCAAAGTCTCCCAGCTATCCTACACAAGCCGAACCGAACACCAATA<br /> |rRNA<br /> |-<br /> |AGACTGGTATTTCAACGACGACTCCACCCACACTGGCGTGCGAGCTTCAAAGTCT<br /> |rRNA<br /> |-<br /> |GTTAGACATCCAGCACGACCAGACTGGTATTTCAACGACGACTC<br /> |rRNA<br /> |-<br /> |TTAAACTACCCATCAGACACTGTCCCTGATCCGGATCACGGACCCAGGTTAGACAT<br /> |rRNA<br /> |-<br /> |GAATCACGGTTGTTTTCTCTTCCTGCGGGTACTGAGATGTTTCACTTC<br /> |rRNA<br /> |-<br /> |GTTACTCTTTAGGAGGCAACCGCCCCAGTTAAACTACCCATCAGACACTG<br /> |rRNA<br /> |-<br /> |ACTCAACACCTGATTGCCAACCAGGCTGAGGGAACCTTTGGGCGCCTCCGTTACT<br /> |rRNA<br /> |-<br /> |GTCTCACAGTCAAGCTCCCTTGTGCACTTACACTCAACACCTGATTG<br /> |rRNA<br /> |-<br /> |GACTTTCGTCCCTGCTCGACCCGTCGGTCTCACAGTCAAG<br /> |rRNA<br /> |-<br /> |TACCTTTTATCCGTTGAGCGACGGCGCTTCCACAAGCCACCGCCGGATCACTAGTC<br /> |rRNA<br /> |-<br /> |GTCGATATGGACTCTTGGGGAAGATCAGCCTGTTATCCCCGGGGTACCTTTTAT<br /> |rRNA<br /> |-<br /> |GACATCGAGGTGCCAAACCATCCCGTCGATATGGACTCTTG<br /> |rRNA<br /> |-<br /> |TTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTCCAGCCCCAGGATGCGACGA<br /> |rRNA<br /> |-<br /> |TCACGACGTTCTAAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACC<br /> |rRNA<br /> |-<br /> |GATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTC<br /> |rRNA<br /> |-<br /> |GTCCTCTCGTACTAGGGACAGCCCTTCTCAAGACTCCTAC<br /> |rRNA<br /> |-<br /> |TCTCACACGTATACGGTTTGGCCTCATCCGGTTTCGCTCGCCACTACTCCCGGAAT<br /> |rRNA<br /> |-<br /> |CATGCCCTTGGCAGGACAACTGGCACACCAGAGGTTCGTC<br /> |rRNA<br /> |-<br /> |CTTAGATGCTTTCAGCGGTTATCCCTCCCGAACGTAGCCAAC<br /> |rRNA<br /> |-<br /> |GTGGGAGTCCTCATCTCGAAGCAGGCTTCCCGCTTAGATG<br /> |rRNA<br /> |-<br /> |CTATCAACCCAGTCGTCTACTGGGAGCCTTAACCCCTCAAGG<br /> |rRNA<br /> |-<br /> |GCCTATTAGTACCGGTCACCTCCACACCTCACGGTGCTTCCAGATCCGGCCTATCAAC<br /> |rRNA<br /> |-<br /> |AGGACAAGCCCTCGGCCTATTAGTACCGGTCACCTCCACA<br /> |rRNA<br /> |-<br /> |CACACGCAACCCCTGCCGGGTCTCACACGTATACGGTTTG<br /> |rRNA<br /> |-<br /> |TTTCTGACTCGTCTCACGGCCGGCAGACCGTAAAAGAGAGATCCCACAACCCCGCACA<br /> |rRNA<br /> |-<br /> |TTTCGCATGTCCTTCGCCTACATCAACGGTTTCTGACTCGTCTCACGGCCGGCAGA<br /> |rRNA<br /> |-<br /> |CTTACCCTCTACGCCGGACCTTTCGCATGTCCTTCGCCTACATC<br /> |rRNA<br /> |-<br /> |GTGTCTCTCAAACGAGCCGTTGACGTTTCGACTACGGGGGTCTTACCCTCTA<br /> |rRNA<br /> |-<br /> |ATCCACCGTGCGCCCTTAAAAACTTGGCCACAGATGCTCG<br /> |rRNA<br /> |-<br /> |GATTTCTCGGGCCCCGTGCTACTTGGGTGTCTCTCAAACGAG<br /> |rRNA<br /> |-<br /> |GAATATTTAGGCTTAGCGGGTGGTCCCGCCAGATTCACACGGGATTTCTC<br /> |rRNA<br /> |-<br /> |CTCACGGTACTATCCGCTATCGGTCACCAGGGAATATTTAGGCTTAG<br /> |rRNA<br /> |-<br /> |GTTTCAGGTACTATTTCACTCCGCTCCCGCGGTACTTTTCACCATTC<br /> |rRNA<br /> |-<br /> |GATGGAGCAAGCTCCATCCCGACGCTCCCACGGCTTGTAGGCACACGGTTTCAGGTACT<br /> |rRNA<br /> |-<br /> |CACGCAGTCACGAGGATGGAGCAAGCTCCATCCCGACGCTC<br /> |rRNA<br /> |-<br /> |CTCGGGTTAACCTCGCAACACACCGCAAACTCGCAGGCTCATTCTTCAAAAGGCACGC<br /> |rRNA<br /> |-<br /> |CTACGGCTTCCCCACTCGGGTTAACCTCGCAACACACCGC<br /> |rRNA<br /> |-<br /> |GTCTTGAGCGTGCTACTAAAAACGCCCTATTCGGACTCGC<br /> |rRNA<br /> |-<br /> |CTCCCACGTCCTTCATCGGTTCCTGGTGCCAAGGCATCCAC<br /> |rRNA<br /> |-<br /> |CTTCAACCTGCCCATGGCTAGATCACTCCGCTTCGGGTCTTGAG<br /> |rRNA<br /> |-<br /> |GGTTCGGTCCTCCACGACCTCTTACAGCCGCTTCAACCTG<br /> |rRNA<br /> |-<br /> |CTAACCACAGGTCATCCCCCAGGTTTTCAACCCTGGTGGGTTCGGTCC<br /> |rRNA<br /> |-<br /> |AGAACCAGCTATCACGGAGTTTGATTGGCCTTTCACCCCTAACCACAGGTCATC<br /> |rRNA<br /> |-<br /> |CAAGAAACACACGACGCTGCACCTAAATGCATTTCGGGGAGAACCAGCTATCA<br /> |rRNA<br /> |-<br /> |TTTTAGCCTTGCGGCCGTACTCCCCAGGCGGGGCACTTAATGCGTTAGCTGCGGCAC<br /> |rRNA<br /> |-<br /> |CCGTCAATTCCTTTGAGTTTTAGCCTTGCGGCCGTACTCC<br /> |rRNA<br /> |-<br /> |AATAATCCGACCGTTCACAGCGTCCTCGCTGTTGTGTTACTTCAAAGGAA<br /> |rRNA<br /> |-<br /> |TTCTTCGCGTTGCGTCGAATTAAGCCACATGCTCCGCCGCTTGTGCGGGCCCCCGTCAA<br /> |rRNA<br /> |-<br /> |GTGTATGTCAAGCCTTGGTAAGGTTCTTCGCGTTGCGTCGAATTAAG<br /> |rRNA<br /> |-<br /> |GGGGGCACCATCTCTGATGCTTTCCGGTGTATGTCAAGCC<br /> |rRNA<br /> |-<br /> |TCACGACACGAGCTGACGACAGCCATGCACCACCTGTACACCGACCACAAGGGGGGCACC<br /> |rRNA<br /> |-<br /> |CTTAACCCAACATCTCACGACACGAGCTGACGACAGCCATG<br /> |rRNA<br /> |-<br /> |CAACACGGGACAAGGGTTGCGCTCGTTGCGGGACTTAACC<br /> |rRNA<br /> |-<br /> |GTCTCCCGTGAGTCCCCAACACCCCGAAGGGCTTGCTGGCAAC<br /> |rRNA<br /> |-<br /> |ACATAAGGGGCATGATGACTTGACGTCGTCCCCACCTTCCTCCGAGTTGACCCCG<br /> |rRNA<br /> |-<br /> |ATTGTACCGGCCATTGTAGCACGTGTGCAGCCCAAGACATAAGGGGCATGATGACTTGA<br /> |rRNA<br /> |-<br /> |TTTTGAGATTCGCTCCACCTTGCGGTATCGCAGCTCATTGTACCGGCCATTGTAGCACGT<br /> |rRNA<br /> |-<br /> |CAATCCGAACTGAGACCGGCTTTTTGAGATTCGCTCCACCTTGCGGTAT<br /> |rRNA<br /> |-<br /> |ATTACTAGCGACTCCGACTTCATGGGGTCGAGTTGCAGACCCCAATCCGAACTGAGA<br /> |rRNA<br /> |-<br /> |TATTCACCGCAGCAATGCTGATCTGCGATTACTAGCGACTCCGACTTCATG<br /> |rRNA<br /> |-<br /> |GTGTACAAGGCCCGGGAACGTATTCACCGCAGCAATGCTG<br /> |rRNA<br /> |-<br /> |GTAATCGGGTGACACCACGAGAGCGGAACAGTCGGAGGAATAA<br /> |rRNA<br /> |-<br /> |GTGTTACCGACTTTCGTGACGTGACGGGCGGTGTGTACAAG<br /> |rRNA<br /> |-<br /> |CACCTTCGACAGCTCCCTCCCACAAGGGGTTGGGCCACCGGCTTCGGGTGTTA<br /> |rRNA<br /> |-<br /> |CTACCTTGTTACGACTTCGTCCCAATCGCCAGTCCCACCTTCGACAG<br /> |rRNA<br /> |-<br /> |TCCTTAGAAAGGAGGTGATCCAGCCGCACCTTCCGGTACGGCTACCTTGTTACGA<br /> |rRNA<br /> |-<br /> |TGCAGTCGGCTATGTGCTCCTTAGAAAGGAGGTGATCCAG<br /> |rRNA<br /> |-<br /> |GATCAAACTCTCCGTGAATGTATACCCGTAATCGGGTGAC<br /> |rRNA<br /> |-<br /> |AAGTGGTTCATCGTTCGACTTGCATGTGTTAAGCACGCCGCCAGCGTTCGTCCTGA<br /> |rRNA<br /> |-<br /> |TATCAACATATCTGGCGTTGACTTTTGGCACGCTGTTGAGTTCTCAAGGAA<br /> |rRNA<br /> |-<br /> |TGTTACTCACCCGTTCGCCACTAATCCCCACCGAAGTGGTTCATCGTTCGACTT<br /> |rRNA<br /> |-<br /> |CTTGTCCCAGAGTGAAGGGCAGATTGCCCACGTGTTACTC<br /> |rRNA<br /> |-<br /> |GCAGATGCCTGCGAGGGTCAGTATCCGGTATTAGACCCCGTTTCCAGGGCTTGTCCCA<br /> |rRNA<br /> |-<br /> |ATCCTTCACCGCCGGAGCTTTCGAACCTCGCAGATGCCTGCGAGGGTCAGTATCC<br /> |rRNA<br /> |-<br /> |GATAGGCCGCGGGCTCATCCTTCACCGCCGGAGCTTTCGA<br /> |rRNA<br /> |-<br /> |GTCGCCTTGGTGAGCCATTACCTCACCAACAAGCTGATAG<br /> |rRNA<br /> |-<br /> |TAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGGCCGGTCGCCCTCTCAGGCCGGCTA<br /> |rRNA<br /> |-<br /> |ATTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTC<br /> |rRNA<br /> |-<br /> |CGTCGCTGCATCAGGCTTTCGCCCATTGTGCAATATTCCC<br /> |rRNA<br /> |-<br /> |CTTCTTCCCTGCTGAAAGAGGTTTACAACCCGAAGGCCGTCATC<br /> |rRNA<br /> |-<br /> |TTCAAAGGAACCTCAACCCGAACGGAGATCCGATCAGGTCGGGGTATCAACATAT<br /> |rRNA<br /> |-<br /> |GTAGTTAGCCGGCGCTTCTTCTGCAGGTACCGTCACTTTC<br /> |rRNA<br /> |-<br /> |ATAATTCCGGACAACGCTTGCGCCCTACGTATTACCGCGGCTGCTGGCACGTAGTTA<br /> |rRNA<br /> |-<br /> |CTTTCACAACCGACGTGACAAGCCGCCTACGAGCTCTTTACGCCCAATAATT<br /> |rRNA<br /> |-<br /> |TATCGACTGCAGACCCGGGGTTAAGCCCCGGGCTTTCACAACCGACGTGACAAG<br /> |rRNA<br /> |-<br /> |GATCTCCCCTACCGAACTCTAGCCTGCCCGTATCGACTGCAGAC<br /> |rRNA<br /> |-<br /> |CTGATATCTGCGCATTTCACCGCTACACCAGGAATTCCGATCTC<br /> |rRNA<br /> |-<br /> |CAGAGATCCGCCTTCGCCACCGGTGTTCCTCCTGATATCTG<br /> |rRNA<br /> |-<br /> |CACGCTTTCGCTCCTCAGCGTCAGTATCGGCCCAGAGATC<br /> |rRNA<br /> |-<br /> |CACCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTTC<br /> |rRNA<br /> |-<br /> |ACTTAATGCGTTAGCTGCGGCACGGACAACGTGGAATGTTGCCCACACCTAGTGCCCA<br /> |rRNA<br /> |-<br /> |GTTCGAAATGTAACCGGGCGTTTCCCCTACGCTATGACCACCGAAA<br /> |rRNA<br /> |-<br /> |CTTAGGATGGTTATAGTTACCACCGCCGTTTACTGGCGCTTAAGTTCTCA<br /> |rRNA<br /> |-<br /> |CCAAGCAAGCTTGATGTCCGACGCTCCCACGGCTTGTAGGCACACGGTTTCAGGTACTA<br /> |rRNA<br /> |-<br /> |CAGTCACGAGACACCAAGCAAGCTTGATGTCCGACGCTCC<br /> |rRNA<br /> |-<br /> |CTTGAGCGTGCTACTAAAAGCGCCCTATTCGGACTCGCTT<br /> |rRNA<br /> |-<br /> |CTACCTTGTTACGACTTCGTCCCAATCGCCAGTCCCACCTTCGACAGCTCCCT<br /> |rRNA<br /> |-<br /> |AAGGAGGTGATCCAGCCGCACCTTCCGGTACGGCTACCTTGTTACGACTTCGTC<br /> |rRNA<br /> |-<br /> |CCAGGATCAAACTCTCCGTGAATGTATACCCGTAATCGGG<br /> |rRNA<br /> |-<br /> |CTACCTTGTTACGACTTCGTCCCAATCGCCAGTCCCACCTTCG<br /> |rRNA<br /> |-<br /> |CTCTCACTTACCGGCATTCGGAGTTTGGCTAAGGTCAGTAAC<br /> |rRNA<br /> |-<br /> |CATTCGGACACCCCCGGATCAAAGCCTGGTTGACGACTCC<br /> |rRNA<br /> |-<br /> |GCTTATCCCCCACAGTCTCACTGCCGCGCTCTCACTTACC<br /> |rRNA<br /> |-<br /> |CTTAGTCGATGCTCTGGGCTGTTTCCCTCTCGACCATGGAGCTTATC<br /> |rRNA<br /> |-<br /> |CACATCCTTTCCCACTTAGCGTACGCTTAGGGGCCTTAGTCGATGCTCTG<br /> |rRNA<br /> |-<br /> |CTTCTAAGCCAACCTCCTGGTTGTCTGTGCGACTCCACATCCTTTC<br /> |rRNA<br /> |-<br /> |AATCACTAGACCAGTGAGCTATTACGCACTCTTTCAAGGGTGGCTGCTTCTAAGC<br /> |rRNA<br /> |-<br /> |CTACATTGTCGGCGCGGAATCACTAGACCAGTGAGCTATTA<br /> |rRNA<br /> |-<br /> |CGTATTGCTGCAATGACACGACTTCGGCGGTACGCTTGAG<br /> |rRNA<br /> |-<br /> |GCTCCCCTACCCATCACAGCCGGCGTTGGCCGTATTGCTG<br /> |rRNA<br /> |-<br /> |TTCTCACTCGTGAACCGTCCACAACTCGCTTCCGCGGCTGCTTCACCCGGCACACGACG<br /> |rRNA<br /> |-<br /> |GTTTGTATCGCTACTCATGCCTGCATTCTCACTCGTGAACCGTCCACAAC<br /> |rRNA<br /> |-<br /> |CTTAGTCAATCGGCGCAAACGTTTCTCACGTTTGTATCGCTACTCATG<br /> |rRNA<br /> |-<br /> |GACTTACCCTGGGCAGATCAGCTTGACCCAGGAACCCTTAGTCAATC<br /> |rRNA<br /> |-<br /> |TCCACACTGCCTATGTGTTCAGCAGCGGGTGACAGCCCATGACGACTGCCGGGTTT<br /> |rRNA<br /> |-<br /> |TTATCCATCGACTACGCCTGTCGGCCTCGCCTTAGGTCCCGACTTACCCTGGGCA<br /> |rRNA<br /> |-<br /> |GTACCGGAATATCAACCGGTTATCCATCGACTACGCCTGTC<br /> |rRNA<br /> |-<br /> |GGCCTTAGCATCACGATGCTCGATGTTTGACGCTTCACAG<br /> |rRNA<br /> |-<br /> |CTACTAACCGCTTGGTTCGGCGGCTCCACCACTCCCCTCAACTCCGAAGAGATCA<br /> |rRNA<br /> |-<br /> |GATGGACTACCTTCCTGCGTCACCCCATCACTCACCTACTAAC<br /> |rRNA<br /> |-<br /> |CCGGCCTACACCCTTACCCCGGGACAACCACCGCCCGGGATGGACTACC<br /> |rRNA<br /> |-<br /> |CAAGGCATCCACCGTGCGCCCTTAAAAACTTGGCCACAGA<br /> |rRNA<br /> |-<br /> |CAAGGTGTGCGGATTTACCTACACACCGGCCTACACCCTTAC<br /> |rRNA<br /> |-<br /> |CCACAATCGGCTCGGCATCAGGTCTCAGCCACAAGGTGTG<br /> |rRNA<br /> |-<br /> |CTAGAGGCTTTTCTCGACAGCATAGGATCATCCACTTCACCACAATC<br /> |rRNA<br /> |-<br /> |TTTAGGGTACGGGCCGCCATGAAACTCGCTAGAGGCTTTTCTC<br /> |rRNA<br /> |-<br /> |CTCGGTATTCTCTACCTGACCACCTGAGTCGGTTTAGGGTAC<br /> |rRNA<br /> |-<br /> |GGCATTTTGCCGAGTTCCTTAACCATAGTTCACCCGAACG<br /> |rRNA<br /> |-<br /> |CAGCTCAGAGTGCAAAACTCGTCACCAGGTGTGGCCCCCCTTCTCCCGAAGTTA<br /> |rRNA<br /> |-<br /> |TTGTTTTCTCTTCCTGCGGGTACTGAGATGTTTCACTTCCCCGCGTTCCCTCCACA<br /> |rRNA<br /> |-<br /> |TGTGTTTTTAGTAAACAGTCGCTTCTCGCTGGTCTCTGCGGCCACCCCCAGCTCAGA<br /> |rRNA<br /> |-<br /> |TATACATCGCCTTACGGCTTCGCACGGACCTGTGTTTTTAGTAAACAGTCGCTTCT<br /> |rRNA<br /> |-<br /> |GCAGGCGTCAGTCCGTATACATCGCCTTACGGCTTCGCAC<br /> |rRNA<br /> |-<br /> |GAAATGTGACTAACCGGTCCCCTTAACGTTCCAGCACCGGGCAGGC<br /> |rRNA<br /> |-<br /> |GTTTACTGGCGCTTAAGTTCTCAGCTTCGCCACACCGAAATGTGACTAAC<br /> |rRNA<br /> |-<br /> |GACAAGGAATTTCGCTACCTTAGGATGGTTATAGTTACCACCGCCGTTTA<br /> |rRNA<br /> |-<br /> |AAGTCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTA<br /> |rRNA<br /> |-<br /> |CTATGGTTGAGACAGTCGAGAAGTCGTTACGCCATTCGTG<br /> |rRNA<br /> |-<br /> |CTGCGCGAAACGAGCATCTTTACTCGTAGTGCAATTTCAC<br /> |rRNA<br /> |-<br /> |CAATATCAAACTGTAGTAAAGGTCCCGGGGTCTTTCCGTCCTGCTGCGCGAAA<br /> |rRNA<br /> |-<br /> |CAGCTATCCTACACAAGCCGAACCGAACACCAATATCAAACTGTAGTAAA<br /> |rRNA<br /> |-<br /> |CACACTGGCGTGCGAGCTTCAAAGTCTCCCAGCTATCCTACACAAG<br /> |rRNA<br /> |-<br /> |TTAGACATCCAGCACGACCAGACTGGTATTTCAACGACGACTCCACCCACACTGGCGTG<br /> |rRNA<br /> |-<br /> |GTTTCGCTCGCCACTACTCCCGGAATCACGGTTGTTTTCTCTTC<br /> |rRNA<br /> |-<br /> |GATCACGGACCCAGGTTAGACATCCAGCACGACCAGACTG<br /> |rRNA<br /> |-<br /> |CAGTTAAACTACCCATCAGACACTGTCCCTGATCCGGATCAC<br /> |rRNA<br /> |-<br /> |GAACCTTTGGGCGCCTCCGTTACTCTTTAGGAGGCAACCG<br /> |rRNA<br /> |-<br /> |CTTGTGCACTTACACTCAACACCTGATTGCCAACCAGGCTGAGGGAACCTTT<br /> |rRNA<br /> |-<br /> |GACCCGTCGGTCTCACAGTCAAGCTCCCTTGTGCACTTAC<br /> |rRNA<br /> |-<br /> |CTTCCACAAGCCACCGCCGGATCACTAGTCCCGACTTTCGTCCCTG<br /> |rRNA<br /> |-<br /> |GTACCTTTTATCCGTTGAGCGACGGCGCTTCCACAAGCCAC<br /> |rRNA<br /> |-<br /> |CAAACCATCCCGTCGATATGGACTCTTGGGGAAGATCAGCCTGTTATC<br /> |rRNA<br /> |-<br /> |CCAGGATGCGACGAGCCGACATCGAGGTGCCAAACCATCC<br /> |rRNA<br /> |-<br /> |GTCTCACACGTATACGGTTTGGCCTCATCCGGTTTCGCTC<br /> |rRNA<br /> |-<br /> |CTTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTCCAG<br /> |rRNA<br /> |-<br /> |GAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCGCTTTAATG<br /> |rRNA<br /> |-<br /> |CTTCTCAAGACTCCTACGCGCACAGCGGATAGGGACCGAAC<br /> |rRNA<br /> |-<br /> |CACACCAGAGGTTCGTCCGTCCCGGTCCTCTCGTACTAGG<br /> |rRNA<br /> |-<br /> |TTAGATGCTTTCAGCGGTTATCCCTCCCGAACGTAGCCAACCAGCCATGCCCTTGGCA<br /> |rRNA<br /> |-<br /> |CTCATCTCGAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATC<br /> |rRNA<br /> |-<br /> |CAGTCGTCTACTGGGAGCCTTAACCCCTCAAGGGGGTGGGAGTCCTCATCTCGAA<br /> |rRNA<br /> |-<br /> |CTTCCAGATCCGGCCTATCAACCCAGTCGTCTACTGGGAG<br /> |rRNA<br /> |-<br /> |CAGACCGTAAAAGAGAGATCCCACAACCCCGCACACGCAACCCCTGCCGGGTCTCA<br /> |rRNA<br /> |-<br /> |TACATCAACGGTTTCTGACTCGTCTCACGGCCGGCAGACCGTAAAAGAGAGAT<br /> |rRNA<br /> |-<br /> |CTTTCGCATGTCCTTCGCCTACATCAACGGTTTCTGACTC<br /> |rRNA<br /> |-<br /> |ACTACGGGGGTCTTACCCTCTACGCCGGACCTTTCGCATG<br /> |rRNA<br /> |-<br /> |CTACTTGGGTGTCTCTCAAACGAGCCGTTGACGTTTCGACTAC<br /> |rRNA<br /> |-<br /> |GCCAGATTCACACGGGATTTCTCGGGCCCCGTGCTACTTG<br /> |rRNA<br /> |-<br /> |TATCCGCTATCGGTCACCAGGGAATATTTAGGCTTAGCGGGTGGTCCCGCCAGATT<br /> |rRNA<br /> |-<br /> |GTACTTTTCACCATTCCCTCACGGTACTATCCGCTATCGGTCAC<br /> |rRNA<br /> |-<br /> |CTTGTAGGCACACGGTTTCAGGTACTATTTCACTCCGCTCCC<br /> |rRNA<br /> |-<br /> |GGGCCTATCGTGGCCTCCCACGTCCTTCATCGGTTCCTGG<br /> |rRNA<br /> |-<br /> |CACGCAGTCACGAGGATGGAGCAAGCTCCATCCCGACGCTCCCACGGCTTGTAGGCA<br /> |rRNA<br /> |-<br /> |CATTCTTCAAAAGGCACGCAGTCACGAGGATGGAGCAAGC<br /> |rRNA<br /> |-<br /> |TACGGCTTCCCCACTCGGGTTAACCTCGCAACACACCGCAAACTCGCAGGCTCAT<br /> |rRNA<br /> |-<br /> |TCGGACTCGCTTTCGCTACGGCTTCCCCACTCGGGTTAAC<br /> |rRNA<br /> |-<br /> |ATCACTCCGCTTCGGGTCTTGAGCGTGCTACTAAAAACGC<br /> |rRNA<br /> |-<br /> |CTCTTACAGCCGCTTCAACCTGCCCATGGCTAGATCACTC<br /> |rRNA<br /> |-<br /> |GTTTTCAACCCTGGTGGGTTCGGTCCTCCACGACCTCTTAC<br /> |rRNA<br /> |-<br /> |GTTTGATTGGCCTTTCACCCCTAACCACAGGTCATCCCCCAGGTTTTCAACC<br /> |rRNA<br /> |-<br /> |GGAGAACCAGCTATCACGGAGTTTGATTGGCCTTTCACCC<br /> |rRNA<br /> |-<br /> |TATCCAGTGCTCTACCTCCGGCAAGAAACACACGACGCTGCACCTAAATGCATTT<br /> |rRNA<br /> |-<br /> |GAGTTTGGCTAAGGTCAGTAACCCGGTAGGGCCCATCGCCTATCCAGTGCTCTA<br /> |rRNA<br /> |-<br /> |CTGTAAGGCTTAGCTTCCGGGTTCGAAATGTAACCGGGCGTTTCCCCTAC<br /> |rRNA<br /> |-<br /> |GTTCGGCGGCGTCCTACTCTCCCACAGGGTCCCCCCTGCAGTACCATCGGCGCTGTA<br /> |rRNA<br /> |-<br /> |GTATTAGACCCCGTTTCCAGGGCTTGTCCCAGAGTGAAGGGCAGATT<br /> |rRNA<br /> |-<br /> |GAGCTTTCGAACCTCGCAGATGCCTGCGAGGGTCAGTATCCGGTATTAGACCCCGTT<br /> |rRNA<br /> |-<br /> |CATCCTTCACCGCCGGAGCTTTCGAACCTCGCAGATGCCT<br /> |rRNA<br /> |-<br /> |CACCGAAGTGGTTCATCGTTCGACTTGCATGTGTTAAGCAC<br /> |rRNA<br /> |-<br /> |TTCGACTTGCATGTGTTAAGCACGCCGCCAGCGTTCGTCCTGAGCCAGGATCAAACTCT<br /> |rRNA<br /> |-<br /> |CAGATTGCCCACGTGTTACTCACCCGTTCGCCACTAATCC<br /> |rRNA<br /> |-<br /> |GCGGATTTACCTACCTGACGTCCTACACCCTTACCCCGGGACAACCACCGC<br /> |rRNA<br /> |-<br /> |CATCAGGTCTCAGACTCATGTCAGGCGGATTTACCTACCTGAC<br /> |rRNA<br /> |-<br /> |CTATTAGTACCGGTCACCTCCACACCTCACGGTGCTTCCAGATCCGGCCTATCAACC<br /> |rRNA<br /> |-<br /> |GGACAAGCCCTCGGCCTATTAGTACCGGTCACCTCCACAC<br /> |rRNA<br /> |-<br /> |CCGGGTTAACCTCGCAACACACCGCAAACTCGCAGGCTCATTCTTCAAAAGGCACGC<br /> |rRNA<br /> |-<br /> |GGTCTTGAGCGTGCTACTAAAACGCCCTATTCGGACTCGC<br /> |rRNA<br /> |-<br /> |TTCGGCGGCGTCCTACTCTCCCACAGGGTCCCCCCTGCAGTACCATCGGCGCTGTAA<br /> |rRNA<br /> |-<br /> |CCGAAGCCTCCGGCGGTCTGCTTTCTGTGGCACTGTCCCG<br /> |SCOs01<br /> |-<br /> |CTGGGCACTGGTGGTCTCTTACACCACCGTTTCACCCTTAC<br /> |SCOs01<br /> |-<br /> |TTCCTTCTTGACCTTGCTCCGGGTGGGGTTTACCTAGCTGCCCAGGTCGCCCTG<br /> |SCOs01<br /> |-<br /> |CTCGAACGTCCGCGCAGAAGCACCGGGGTGCTTCCTTCTTGACCTTGCTC<br /> |SCOs01<br /> |-<br /> |CTCCAGCGGTCTACCCGCGGACTCGGGCGGGCAGCCCTCGAACGTCCGCGCAGAAG<br /> |SCOs01<br /> |-<br /> |CATCCATCTAGGACCGGCGTTGCCACCGGCCTCCAGCGGTCTAC<br /> |SCOs01<br /> |-<br /> |CTGTGGAGCCCGGACGTTCCTCGGGAAGTCCCGTGAGGGACTC<br /> |SCOs01<br /> |-<br /> |GTTCTGTTCCGACGGGGCCTCGCGGTCCCGTCGGCGACGGCCATCCAT<br /> |SCOs01<br /> |-<br /> |CAGACGAGTCGGGCTGTACGCCGGGTTCTGTTCCGACGGG<br /> |SCOs01<br /> |-<br /> |GTCTGCTTTCTGTGGCACTGTCCCGCGGGTCACCCCGGGTGGCCGTTAGCCATCAC<br /> |SCOs01<br /> |-<br /> |CTTAGTAGGAGGCTCGCTTCGCTACCTGAGATCAGGCAGCGAGGGCGAAGG<br /> |SCOs02<br /> |-<br /> |GAACAGCCCCGGGCTGACACTCCCTTAGTAGGAGGCTCGCTTC<br /> |SCOs02<br /> |-<br /> |GATCAAGGTTTAGTCCCCTAGCTGATGCCAGGATCCGGGTC<br /> |SCOs02<br /> |-<br /> |CAGTCACTGTTTGGTTTCCCTCTTCACCCCGTGACCGGGATCAA<br /> |SCOs02<br /> |-<br /> |GAGATCACGCGGACAAGTCTCCGACGGGCCCAGTCACTGT<br /> |SCOs02<br /> |-<br /> |CTTCCCCTGCTTCGACAGCCGCTGTCGAAACCGATCATCC<br /> |SCOs02<br /> |-<br /> |AATCAGGGCTTCTCCGTGTGCAGTCCGCTTCGATTTTCTCGGCCCCGGAGATCACG<br /> |SCOs02<br /> |-<br /> |GAATCGAACCCGCGTCCAACGGTGCGGAATCAGGGCTTCTC<br /> |SCOs02<br /> |-<br /> |TTTTTCGGCCTGTGGTTTACGAGATCATGGCCGCTTCCTC<br /> |SCOs02<br /> |-<br /> |CTGCTGGGAGGAATCGCGCTTGGTGTTGGCGATTATTTTT<br /> |SCOs02<br /> |-<br /> |CACGCACCCGCCAGAGCCGACCTACCCTTGCTGCCTTCCG<br /> |SCOs03<br /> |}</div> RTEvans https://actinobase.org/index.php?title=RRNA_depletion_with_Streptomyces_coelicolor_A3(2)_specific_probes&diff=2190 RRNA depletion with Streptomyces coelicolor A3(2) specific probes 2023-11-01T16:42:15Z <p>RTEvans: RTEvans moved page RRNA depletion with Streptomyces coelicolor A3(2) specific probes to RRNA depletion with Streptomyces coelicolor A3(2) specific probes.: Removing capitalised R in rRNA</p> <hr /> <div>==Organisms==<br /> <br /> This protocol has been tested with &lt;em&gt;[[Streptomyces coelicolor]]&lt;/em&gt; A3(2) MT1110<br /> <br /> ==Description==<br /> <br /> rRNA depletion using commercial kits targeting generic bacterial sequences is not 100% efficient in the High GC organism S. coelicolor. <br /> <br /> Depletion using the NEBNext RNA Depletion Core Reagent Set with RNA Sample Purification Beads (https://www.neb.com/en-gb/products/e7870-nebnext-rna-depletion-core-reagent-set-with-rna-sample-purification-beads#Product%20Information) and custom DNA probes designed for &lt;em&gt;S. coelicolor&lt;/em&gt; is more effective than the QIAseq FastSelect 5S/16S/23S kit (Qiagen #335927); NEBNext rRNA Depletion Kit (Bacteria) (NEB #E7850); Illumina Ribo-zero rRNA Removal Kit (Bacteria) (Illumina #MRZMB12424, discontinued). The Illumina kit tested was subsequently replaced by the Illumina Ribo-Zero Plus rRNA Depletion Kit (Illumina #20040526). Additionally three abundant non-mRNA RNAs can be depleted (Table 1).<br /> <br /> '''Table 1.''' Abundant non-mRNA RNAs in &lt;em&gt;S. coelicolor&lt;/em&gt;.<br /> <br /> [[File: rRNA_depletion_table_1.png]]<br /> <br /> ==Materials==<br /> <br /> Single stranded DNA probes (Table 2) at 2µM concentration in IDTE pH 7.5 (10mM Tris-HCl / 0.1mM EDTA). Integrated DNA Technologies can produce these from their 25nmole DNA oligo pool product. Probes are listed in Table 2 in the Notes section below.<br /> <br /> NEBNext RNA Depletion Core Reagent Set with RNA Sample Purification Beads (https://www.neb.com/en-gb/products/e7870-nebnext-rna-depletion-core-reagent-set-with-rna-sample-purification-beads#Product%20Information).<br /> <br /> ==Protocols==<br /> <br /> As per manufacturer’s instructions.<br /> <br /> ==Notes==<br /> <br /> The following histograms illustrate effectiveness of rRNA depletion using various methods. Histograms show percentage of bases aligned to NCBI reference sequence NC_003888.3 using Hisat2 program classified as follows - Coding = RNA aligned to coding sequences; Transcribed not translated = RNA aligned to rRNA, tRNA and sRNA; Intergenic = RNA aligned to 5' or 3' UTRs or between annotated sequences.<br /> <br /> '''QIAseq FastSelect 5S/16S/23S kit'''<br /> <br /> [[File: PanelA.png]] <br /> <br /> '''NEBNext rRNA Depletion Kit (Bacteria)'''<br /> <br /> [[File: PanelB.png]]<br /> <br /> '''Illumina Ribo-zero rRNA Removal Kit (Bacteria)'''<br /> <br /> [[File: PanelC.png]] <br /> <br /> '''rRNA depletion with &lt;em&gt;S. coelicolor&lt;/em&gt; specific probes<br /> <br /> [[File: PanelD.png]]<br /> <br /> <br /> '''Table 2''' List of probes<br /> <br /> {| class=&quot;wikitable&quot;<br /> !Sequence<br /> !Target<br /> |-<br /> |CAAGTCCTTGCGCGACGCTCCCACGGCTTGTAGGCACACGGTTTCAGGTACTATTT<br /> |rRNA<br /> |-<br /> |CAGTCACGACGCAAGGACAAGTCCTTGCGCGACGCTCCCAC<br /> |rRNA<br /> |-<br /> |ACCTCGCAACACACCGCAAACTCGCAGGCTCATTCTTCAAAAGGCACGCAGTCACGACG<br /> |rRNA<br /> |-<br /> |CTACGGCTTCCCCACCCGGGTTAACCTCGCAACACACCGC<br /> |rRNA<br /> |-<br /> |TTAACCCAACATCTCACGACACGAGCTGACGACAGCCATGCACCACCTGTACACCGA<br /> |rRNA<br /> |-<br /> |CGCTCGTTGCGGGACTTAACCCAACATCTCACGACACGAG<br /> |rRNA<br /> |-<br /> |CAACACCCCGAAGGGCTTGCTGGCAACACGGGACAAGGGTTG<br /> |rRNA<br /> |-<br /> |CACCTTCCTCCGAGTTGACCCCGGCGGTCTCCCGTGAGTC<br /> |rRNA<br /> |-<br /> |GACATAAGGGGCATGATGACTTGACGTCGTCCCCACCTTC<br /> |rRNA<br /> |-<br /> |GTATCGCAGCTCATTGTACCGGCCATTGTAGCACGTGTGCAGCCCAAGACATAA<br /> |rRNA<br /> |-<br /> |TTTTGAGATTCGCTCCACCTTGCGGTATCGCAGCTCATTGTAC<br /> |rRNA<br /> |-<br /> |CAGACCCCAATCCGAACTGAGACCGGCTTTTTGAGATTCG<br /> |rRNA<br /> |-<br /> |AATGCTGATCTGCGATTACTAGCGACTCCGACTTCATGGGGTCGAGTTGCAGAC<br /> |rRNA<br /> |-<br /> |GAACGTATTCACCGCAGCAATGCTGATCTGCGATTACTAG<br /> |rRNA<br /> |-<br /> |TACCGACTTTCGTGACGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTC<br /> |rRNA<br /> |-<br /> |GCCACCGGCTTCGGGTGTTACCGACTTTCGTGACGTGACG<br /> |rRNA<br /> |-<br /> |TTCGTCCCAATCGCCAGTCCCACCTTCGACAGCTCCCTCCCACAAGGGGTTGGG<br /> |rRNA<br /> |-<br /> |CGCACCTTCCGGTACGGCTACCTTGTTACGACTTCGTCCCAATCGCCAGTCCCA<br /> |rRNA<br /> |-<br /> |TTCGACCCTCGCAGATGCCTGCGAGGGTCAGTATCCGGTATTAGACCCCGTTTCCAG<br /> |rRNA<br /> |-<br /> |GAAAGGAGGTGATCCAGCCGCACCTTCCGGTACGGCTACC<br /> |rRNA<br /> |-<br /> |TTCACCGCCGGAGCTTTCGACCCTCGCAGATGCCTGCGAG<br /> |rRNA<br /> |-<br /> |CATTACCTCACCAACAAGCTGATAGGCCGCGGGCTCATCCTTC<br /> |rRNA<br /> |-<br /> |CTCTCAGGCCGGCTACCCGTCGTCGCCTTGGTGAGCCATTAC<br /> |rRNA<br /> |-<br /> |GTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGGCCGGTCG<br /> |rRNA<br /> |-<br /> |TGCATCAGGCTTTCGCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCT<br /> |rRNA<br /> |-<br /> |TGCTGAAAGAGGTTTACAACCCGAAGGCCGTCATCCCTCACGCGGCGTCGCTGCATCA<br /> |rRNA<br /> |-<br /> |TTCTTCTGCAGGTACCGTCACTTTCGCTTCTTCCCTGCTGAAAGAGGTTTACAA<br /> |rRNA<br /> |-<br /> |CTACGTATTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTCTG<br /> |rRNA<br /> |-<br /> |CTCTTTACGCCCAATAATTCCGGACAACGCTTGCGCCCTACGTATTAC<br /> |rRNA<br /> |-<br /> |GGGCTTTCACAACCGACGTGACAAGCCGCCTACGAGCTCT<br /> |rRNA<br /> |-<br /> |CTACCGAACTCTAGCCTGCCCGTATCGACTGCAGACCCGGGGTTAAGCCCCG<br /> |rRNA<br /> |-<br /> |ATATCTGCGCATTTCACCGCTACACCAGGAATTCCGATCTCCCCTACCGAACTCTAG<br /> |rRNA<br /> |-<br /> |CGGTGTTCCTCCTGATATCTGCGCATTTCACCGCTACACC<br /> |rRNA<br /> |-<br /> |ACGCTTTCGCTCCTCAGCGTCAGTATCGGCCCAGAGATCCGCCTTCGCCACCGGTGT<br /> |rRNA<br /> |-<br /> |GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCG<br /> |rRNA<br /> |-<br /> |ACAACGTGGAATGTTGCCCACACCTAGTGCCCACCGTTTACGGCGTGGACTACCA<br /> |rRNA<br /> |-<br /> |CTTAATGCGTTAGCTGCGGCACGGACAACGTGGAATGTTG<br /> |rRNA<br /> |-<br /> |TCAATTCCTTTGAGTTTTAGCCTTGCGGCCGTACTCCCCAGGCGGGGCACTTAA<br /> |rRNA<br /> |-<br /> |ATTAAGCCACATGCTCCGCCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTTA<br /> |rRNA<br /> |-<br /> |TCAAGCCTTGGTAAGGTTCTTCGCGTTGCGTCGAATTAAGCCACATGCTCCGCCGCTT<br /> |rRNA<br /> |-<br /> |CATCTCTGATGCTTTCCGGTGTATGTCAAGCCTTGGTAAGGTTCTTC<br /> |rRNA<br /> |-<br /> |ACAGCCATGCACCACCTGTACACCGACCACAAGGGGGGCACCATCTCTGATGCTTTCC<br /> |rRNA<br /> |-<br /> |TGTAACCGGGCGTTTCCCCTACGCTATGACCACCGAAACC<br /> |rRNA<br /> |-<br /> |CAGTACCATCGGCGCTGTAAGGCTTAGCTTCCGGGTTCGAAATGTAAC<br /> |rRNA<br /> |-<br /> |GTTCGGCGGCGTCCTACTCTCCCACAGGGTCCCCCCTGCA<br /> |rRNA<br /> |-<br /> |CACTCCCCTCAACTCCGAAGAGATCAGGGCGGCTTCACGG<br /> |rRNA<br /> |-<br /> |CCATCACTCACCTACTAACCGCTTGGTTCGGCGGCTCCAC<br /> |rRNA<br /> |-<br /> |GTCCTTCATCGGTTCCTGGTGCCAAGGCATCCACCGTGCGCCCTTAAAAACTT<br /> |rRNA<br /> |-<br /> |CTTGTAGGCACACGGTTTCAGGTACTATTTCACTCCGCTCCCG<br /> |rRNA<br /> |-<br /> |CACGCAGTCACGAGACACCAAGCAAGCTTGATGTCCGACGCTCCCACGGCTTGTAGGCAC<br /> |rRNA<br /> |-<br /> |TCTTCAAAAGGCACGCAGTCACGAGACACCAAGCAAGCTT<br /> |rRNA<br /> |-<br /> |CACCCGGGTTAACCTCGCAACACACTGCAAACTCGCAGGC<br /> |rRNA<br /> |-<br /> |GCGTGCTACTAAAACGCCCTATTCGGACTCGCTTTCGCTACGGCTTCC<br /> |rRNA<br /> |-<br /> |GATCACTCCGCTTCGGGTCTTGAGCGTGCTACTAAAACGC<br /> |rRNA<br /> |-<br /> |TCGCAGATGCCTGCAAGGGTCAGTATCCGGTATTAGACCCCGTTT<br /> |rRNA<br /> |-<br /> |TCATCCTTCACCGCCGCAGCTTTCGAACCTCGCAGATGCC<br /> |rRNA<br /> |-<br /> |ATGTAACCGGGCGTTTCCCCTACGCTATGACCACCGAAAC<br /> |rRNA<br /> |-<br /> |CGTCCTACTCTCCCACAGGGTCCCCCCTGCAGTACCATCG<br /> |rRNA<br /> |-<br /> |CATCGCCTATCCAGTGCTCTACCTCCGGCAAGAAACACAC<br /> |rRNA<br /> |-<br /> |CATTCGGAGTTTGGCTAAGGTCAGTAACCCGGTAGGGCCCATCG<br /> |rRNA<br /> |-<br /> |CAGTCTCACTGCCGCGCTCTCACTTACCGGCATTCGGAGTTTG<br /> |rRNA<br /> |-<br /> |ATGCTCTGGGCTGTTTCCCTCTCGACCATGGAGCTTATCCCCCACAGTCTCACTG<br /> |rRNA<br /> |-<br /> |CTTAGGGGCCTTAGTCGATGCTCTGGGCTGTTTCCCTCTCG<br /> |rRNA<br /> |-<br /> |GTTGTCTGTGCGACTCCACATCCTTTCCCACTTAGCGTACGCTTAG<br /> |rRNA<br /> |-<br /> |TATTACGCACTCTTTCAAGGGTGGCTGCTTCTAAGCCAACCTCCTGGTTGTCTGT<br /> |rRNA<br /> |-<br /> |GATCAAAGCCTGGTTGACGACTCCCCGGGGCCTATCGTGG<br /> |rRNA<br /> |-<br /> |CGGAATCACTAGACCAGTGAGCTATTACGCACTCTTTCAAGG<br /> |rRNA<br /> |-<br /> |TATTGCTGCAATGACACGACTTCGGCGGTACGCTTGAGCCCCGCTACATTGTCG<br /> |rRNA<br /> |-<br /> |CATCACAGCCGGCGTTGGCCGTATTGCTGCAATGACACGACTTC<br /> |rRNA<br /> |-<br /> |TCCACAACTCGCTTCCGCGGCTGCTTCACCCGGCACACGACGCTCCCCTACCCATCACA<br /> |rRNA<br /> |-<br /> |TACTCATGCCTGCATTCTCACTCGTGAACCGTCCACAACTCGCTTCCGCGGCTGCTT<br /> |rRNA<br /> |-<br /> |CAAACGTTTCTCACGTTTGTATCGCTACTCATGCCTGCATTCTCACTC<br /> |rRNA<br /> |-<br /> |CAGGAACCCTTAGTCAATCGGCGCAAACGTTTCTCACGTTT<br /> |rRNA<br /> |-<br /> |CTTAGGTCCCGACTTACCCTGGGCAGATCAGCTTGACCCAG<br /> |rRNA<br /> |-<br /> |GAATATCAACCGGTTATCCATCGACTACGCCTGTCGGCCTCGCCTTAGGTC<br /> |rRNA<br /> |-<br /> |TTAGCATCACGATGCTCGATGTTTGACGCTTCACAGCGGGTACCGGAATATCAA<br /> |rRNA<br /> |-<br /> |GAAGAGATCAGGGCGGCTTCACGGCCTTAGCATCACGATG<br /> |rRNA<br /> |-<br /> |CATCACTCACCTACTAACCGCTTGGTTCGGCGGCTCCACC<br /> |rRNA<br /> |-<br /> |TGTGTTCAGCAGCGGGTGACAGCCCATGACGACTGCCGGGTTTCCCCATTCGGACACCC<br /> |rRNA<br /> |-<br /> |GACAACCACCGCCCGGGATGGACTACCTTCCTGCGTCACCCCAT<br /> |rRNA<br /> |-<br /> |CAGATGCTCGCGTCCACTGTGCAGTTCTCAAACAACGACC<br /> |rRNA<br /> |-<br /> |TGCGGATTTACCTACACACCGGCCTACACCCTTACCCCGGGACAACCACCG<br /> |rRNA<br /> |-<br /> |CATCAGGTCTCAGCCACAAGGTGTGCGGATTTACCTACAC<br /> |rRNA<br /> |-<br /> |TTTTCTCGACAGCATAGGATCATCCACTTCACCACAATCGGCTCGGCATCAGGTCT<br /> |rRNA<br /> |-<br /> |CCATGAAACTCGCTAGAGGCTTTTCTCGACAGCATAGGATCATC<br /> |rRNA<br /> |-<br /> |TATTCTCTACCTGACCACCTGAGTCGGTTTAGGGTACGGGCCGCCATGAAACT<br /> |rRNA<br /> |-<br /> |GAGTTCCTTAACCATAGTTCACCCGAACGCCTCGGTATTCTCTACCTGAC<br /> |rRNA<br /> |-<br /> |CTTCTCCCGAAGTTACGGGGGCATTTTGCCGAGTTCCTTAACCA<br /> |rRNA<br /> |-<br /> |CACCCCCAGCTCAGAGTGCAAAACTCGTCACCAGGTGTGG<br /> |rRNA<br /> |-<br /> |GTGTTTTTAGTAAACAGTCGCTTCTCGCTGGTCTCTGCGG<br /> |rRNA<br /> |-<br /> |GTTCCCTCCACACTGCCTATGTGTTCAGCAGCGGGTGACAG<br /> |rRNA<br /> |-<br /> |GTATACATCGCCTTACGGCTTCGCACGGACCTGTGTTTTTA<br /> |rRNA<br /> |-<br /> |CTTAACGTTCCAGCACCGGGCAGGCGTCAGTCCGTATACATC<br /> |rRNA<br /> |-<br /> |TAAGTTCTCAGCTTCGCCACACCGAAATGTGACTAACCGGTCCCCTTAACGTT<br /> |rRNA<br /> |-<br /> |TTAGGATGGTTATAGTTACCACCGCCGTTTACTGGCGCTTAAGTTCTCAG<br /> |rRNA<br /> |-<br /> |GTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGATGGTTATAGT<br /> |rRNA<br /> |-<br /> |TATGGTTGAGACAGTCGAGAAGTCGTTACGCCATTCGTGCAGGTCGGAACTTACC<br /> |rRNA<br /> |-<br /> |GAAACGAGCATCTTTACTCGTAGTGCAATTTCACCGGGCCTATGGTTGAG<br /> |rRNA<br /> |-<br /> |GGTCTTTCCGTCCTGCTGCGCGAAACGAGCATCTTTACTC<br /> |rRNA<br /> |-<br /> |GAACCGAACACCAATATCAAACTGTAGTAAAGGTCCCGGGGTCTTTC<br /> |rRNA<br /> |-<br /> |CTTCAAAGTCTCCCAGCTATCCTACACAAGCCGAACCGAACACCAATA<br /> |rRNA<br /> |-<br /> |AGACTGGTATTTCAACGACGACTCCACCCACACTGGCGTGCGAGCTTCAAAGTCT<br /> |rRNA<br /> |-<br /> |GTTAGACATCCAGCACGACCAGACTGGTATTTCAACGACGACTC<br /> |rRNA<br /> |-<br /> |TTAAACTACCCATCAGACACTGTCCCTGATCCGGATCACGGACCCAGGTTAGACAT<br /> |rRNA<br /> |-<br /> |GAATCACGGTTGTTTTCTCTTCCTGCGGGTACTGAGATGTTTCACTTC<br /> |rRNA<br /> |-<br /> |GTTACTCTTTAGGAGGCAACCGCCCCAGTTAAACTACCCATCAGACACTG<br /> |rRNA<br /> |-<br /> |ACTCAACACCTGATTGCCAACCAGGCTGAGGGAACCTTTGGGCGCCTCCGTTACT<br /> |rRNA<br /> |-<br /> |GTCTCACAGTCAAGCTCCCTTGTGCACTTACACTCAACACCTGATTG<br /> |rRNA<br /> |-<br /> |GACTTTCGTCCCTGCTCGACCCGTCGGTCTCACAGTCAAG<br /> |rRNA<br /> |-<br /> |TACCTTTTATCCGTTGAGCGACGGCGCTTCCACAAGCCACCGCCGGATCACTAGTC<br /> |rRNA<br /> |-<br /> |GTCGATATGGACTCTTGGGGAAGATCAGCCTGTTATCCCCGGGGTACCTTTTAT<br /> |rRNA<br /> |-<br /> |GACATCGAGGTGCCAAACCATCCCGTCGATATGGACTCTTG<br /> |rRNA<br /> |-<br /> |TTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTCCAGCCCCAGGATGCGACGA<br /> |rRNA<br /> |-<br /> |TCACGACGTTCTAAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACC<br /> |rRNA<br /> |-<br /> |GATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTC<br /> |rRNA<br /> |-<br /> |GTCCTCTCGTACTAGGGACAGCCCTTCTCAAGACTCCTAC<br /> |rRNA<br /> |-<br /> |TCTCACACGTATACGGTTTGGCCTCATCCGGTTTCGCTCGCCACTACTCCCGGAAT<br /> |rRNA<br /> |-<br /> |CATGCCCTTGGCAGGACAACTGGCACACCAGAGGTTCGTC<br /> |rRNA<br /> |-<br /> |CTTAGATGCTTTCAGCGGTTATCCCTCCCGAACGTAGCCAAC<br /> |rRNA<br /> |-<br /> |GTGGGAGTCCTCATCTCGAAGCAGGCTTCCCGCTTAGATG<br /> |rRNA<br /> |-<br /> |CTATCAACCCAGTCGTCTACTGGGAGCCTTAACCCCTCAAGG<br /> |rRNA<br /> |-<br /> |GCCTATTAGTACCGGTCACCTCCACACCTCACGGTGCTTCCAGATCCGGCCTATCAAC<br /> |rRNA<br /> |-<br /> |AGGACAAGCCCTCGGCCTATTAGTACCGGTCACCTCCACA<br /> |rRNA<br /> |-<br /> |CACACGCAACCCCTGCCGGGTCTCACACGTATACGGTTTG<br /> |rRNA<br /> |-<br /> |TTTCTGACTCGTCTCACGGCCGGCAGACCGTAAAAGAGAGATCCCACAACCCCGCACA<br /> |rRNA<br /> |-<br /> |TTTCGCATGTCCTTCGCCTACATCAACGGTTTCTGACTCGTCTCACGGCCGGCAGA<br /> |rRNA<br /> |-<br /> |CTTACCCTCTACGCCGGACCTTTCGCATGTCCTTCGCCTACATC<br /> |rRNA<br /> |-<br /> |GTGTCTCTCAAACGAGCCGTTGACGTTTCGACTACGGGGGTCTTACCCTCTA<br /> |rRNA<br /> |-<br /> |ATCCACCGTGCGCCCTTAAAAACTTGGCCACAGATGCTCG<br /> |rRNA<br /> |-<br /> |GATTTCTCGGGCCCCGTGCTACTTGGGTGTCTCTCAAACGAG<br /> |rRNA<br /> |-<br /> |GAATATTTAGGCTTAGCGGGTGGTCCCGCCAGATTCACACGGGATTTCTC<br /> |rRNA<br /> |-<br /> |CTCACGGTACTATCCGCTATCGGTCACCAGGGAATATTTAGGCTTAG<br /> |rRNA<br /> |-<br /> |GTTTCAGGTACTATTTCACTCCGCTCCCGCGGTACTTTTCACCATTC<br /> |rRNA<br /> |-<br /> |GATGGAGCAAGCTCCATCCCGACGCTCCCACGGCTTGTAGGCACACGGTTTCAGGTACT<br /> |rRNA<br /> |-<br /> |CACGCAGTCACGAGGATGGAGCAAGCTCCATCCCGACGCTC<br /> |rRNA<br /> |-<br /> |CTCGGGTTAACCTCGCAACACACCGCAAACTCGCAGGCTCATTCTTCAAAAGGCACGC<br /> |rRNA<br /> |-<br /> |CTACGGCTTCCCCACTCGGGTTAACCTCGCAACACACCGC<br /> |rRNA<br /> |-<br /> |GTCTTGAGCGTGCTACTAAAAACGCCCTATTCGGACTCGC<br /> |rRNA<br /> |-<br /> |CTCCCACGTCCTTCATCGGTTCCTGGTGCCAAGGCATCCAC<br /> |rRNA<br /> |-<br /> |CTTCAACCTGCCCATGGCTAGATCACTCCGCTTCGGGTCTTGAG<br /> |rRNA<br /> |-<br /> |GGTTCGGTCCTCCACGACCTCTTACAGCCGCTTCAACCTG<br /> |rRNA<br /> |-<br /> |CTAACCACAGGTCATCCCCCAGGTTTTCAACCCTGGTGGGTTCGGTCC<br /> |rRNA<br /> |-<br /> |AGAACCAGCTATCACGGAGTTTGATTGGCCTTTCACCCCTAACCACAGGTCATC<br /> |rRNA<br /> |-<br /> |CAAGAAACACACGACGCTGCACCTAAATGCATTTCGGGGAGAACCAGCTATCA<br /> |rRNA<br /> |-<br /> |TTTTAGCCTTGCGGCCGTACTCCCCAGGCGGGGCACTTAATGCGTTAGCTGCGGCAC<br /> |rRNA<br /> |-<br /> |CCGTCAATTCCTTTGAGTTTTAGCCTTGCGGCCGTACTCC<br /> |rRNA<br /> |-<br /> |AATAATCCGACCGTTCACAGCGTCCTCGCTGTTGTGTTACTTCAAAGGAA<br /> |rRNA<br /> |-<br /> |TTCTTCGCGTTGCGTCGAATTAAGCCACATGCTCCGCCGCTTGTGCGGGCCCCCGTCAA<br /> |rRNA<br /> |-<br /> |GTGTATGTCAAGCCTTGGTAAGGTTCTTCGCGTTGCGTCGAATTAAG<br /> |rRNA<br /> |-<br /> |GGGGGCACCATCTCTGATGCTTTCCGGTGTATGTCAAGCC<br /> |rRNA<br /> |-<br /> |TCACGACACGAGCTGACGACAGCCATGCACCACCTGTACACCGACCACAAGGGGGGCACC<br /> |rRNA<br /> |-<br /> |CTTAACCCAACATCTCACGACACGAGCTGACGACAGCCATG<br /> |rRNA<br /> |-<br /> |CAACACGGGACAAGGGTTGCGCTCGTTGCGGGACTTAACC<br /> |rRNA<br /> |-<br /> |GTCTCCCGTGAGTCCCCAACACCCCGAAGGGCTTGCTGGCAAC<br /> |rRNA<br /> |-<br /> |ACATAAGGGGCATGATGACTTGACGTCGTCCCCACCTTCCTCCGAGTTGACCCCG<br /> |rRNA<br /> |-<br /> |ATTGTACCGGCCATTGTAGCACGTGTGCAGCCCAAGACATAAGGGGCATGATGACTTGA<br /> |rRNA<br /> |-<br /> |TTTTGAGATTCGCTCCACCTTGCGGTATCGCAGCTCATTGTACCGGCCATTGTAGCACGT<br /> |rRNA<br /> |-<br /> |CAATCCGAACTGAGACCGGCTTTTTGAGATTCGCTCCACCTTGCGGTAT<br /> |rRNA<br /> |-<br /> |ATTACTAGCGACTCCGACTTCATGGGGTCGAGTTGCAGACCCCAATCCGAACTGAGA<br /> |rRNA<br /> |-<br /> |TATTCACCGCAGCAATGCTGATCTGCGATTACTAGCGACTCCGACTTCATG<br /> |rRNA<br /> |-<br /> |GTGTACAAGGCCCGGGAACGTATTCACCGCAGCAATGCTG<br /> |rRNA<br /> |-<br /> |GTAATCGGGTGACACCACGAGAGCGGAACAGTCGGAGGAATAA<br /> |rRNA<br /> |-<br /> |GTGTTACCGACTTTCGTGACGTGACGGGCGGTGTGTACAAG<br /> |rRNA<br /> |-<br /> |CACCTTCGACAGCTCCCTCCCACAAGGGGTTGGGCCACCGGCTTCGGGTGTTA<br /> |rRNA<br /> |-<br /> |CTACCTTGTTACGACTTCGTCCCAATCGCCAGTCCCACCTTCGACAG<br /> |rRNA<br /> |-<br /> |TCCTTAGAAAGGAGGTGATCCAGCCGCACCTTCCGGTACGGCTACCTTGTTACGA<br /> |rRNA<br /> |-<br /> |TGCAGTCGGCTATGTGCTCCTTAGAAAGGAGGTGATCCAG<br /> |rRNA<br /> |-<br /> |GATCAAACTCTCCGTGAATGTATACCCGTAATCGGGTGAC<br /> |rRNA<br /> |-<br /> |AAGTGGTTCATCGTTCGACTTGCATGTGTTAAGCACGCCGCCAGCGTTCGTCCTGA<br /> |rRNA<br /> |-<br /> |TATCAACATATCTGGCGTTGACTTTTGGCACGCTGTTGAGTTCTCAAGGAA<br /> |rRNA<br /> |-<br /> |TGTTACTCACCCGTTCGCCACTAATCCCCACCGAAGTGGTTCATCGTTCGACTT<br /> |rRNA<br /> |-<br /> |CTTGTCCCAGAGTGAAGGGCAGATTGCCCACGTGTTACTC<br /> |rRNA<br /> |-<br /> |GCAGATGCCTGCGAGGGTCAGTATCCGGTATTAGACCCCGTTTCCAGGGCTTGTCCCA<br /> |rRNA<br /> |-<br /> |ATCCTTCACCGCCGGAGCTTTCGAACCTCGCAGATGCCTGCGAGGGTCAGTATCC<br /> |rRNA<br /> |-<br /> |GATAGGCCGCGGGCTCATCCTTCACCGCCGGAGCTTTCGA<br /> |rRNA<br /> |-<br /> |GTCGCCTTGGTGAGCCATTACCTCACCAACAAGCTGATAG<br /> |rRNA<br /> |-<br /> |TAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGGCCGGTCGCCCTCTCAGGCCGGCTA<br /> |rRNA<br /> |-<br /> |ATTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTC<br /> |rRNA<br /> |-<br /> |CGTCGCTGCATCAGGCTTTCGCCCATTGTGCAATATTCCC<br /> |rRNA<br /> |-<br /> |CTTCTTCCCTGCTGAAAGAGGTTTACAACCCGAAGGCCGTCATC<br /> |rRNA<br /> |-<br /> |TTCAAAGGAACCTCAACCCGAACGGAGATCCGATCAGGTCGGGGTATCAACATAT<br /> |rRNA<br /> |-<br /> |GTAGTTAGCCGGCGCTTCTTCTGCAGGTACCGTCACTTTC<br /> |rRNA<br /> |-<br /> |ATAATTCCGGACAACGCTTGCGCCCTACGTATTACCGCGGCTGCTGGCACGTAGTTA<br /> |rRNA<br /> |-<br /> |CTTTCACAACCGACGTGACAAGCCGCCTACGAGCTCTTTACGCCCAATAATT<br /> |rRNA<br /> |-<br /> |TATCGACTGCAGACCCGGGGTTAAGCCCCGGGCTTTCACAACCGACGTGACAAG<br /> |rRNA<br /> |-<br /> |GATCTCCCCTACCGAACTCTAGCCTGCCCGTATCGACTGCAGAC<br /> |rRNA<br /> |-<br /> |CTGATATCTGCGCATTTCACCGCTACACCAGGAATTCCGATCTC<br /> |rRNA<br /> |-<br /> |CAGAGATCCGCCTTCGCCACCGGTGTTCCTCCTGATATCTG<br /> |rRNA<br /> |-<br /> |CACGCTTTCGCTCCTCAGCGTCAGTATCGGCCCAGAGATC<br /> |rRNA<br /> |-<br /> |CACCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTTC<br /> |rRNA<br /> |-<br /> |ACTTAATGCGTTAGCTGCGGCACGGACAACGTGGAATGTTGCCCACACCTAGTGCCCA<br /> |rRNA<br /> |-<br /> |GTTCGAAATGTAACCGGGCGTTTCCCCTACGCTATGACCACCGAAA<br /> |rRNA<br /> |-<br /> |CTTAGGATGGTTATAGTTACCACCGCCGTTTACTGGCGCTTAAGTTCTCA<br /> |rRNA<br /> |-<br /> |CCAAGCAAGCTTGATGTCCGACGCTCCCACGGCTTGTAGGCACACGGTTTCAGGTACTA<br /> |rRNA<br /> |-<br /> |CAGTCACGAGACACCAAGCAAGCTTGATGTCCGACGCTCC<br /> |rRNA<br /> |-<br /> |CTTGAGCGTGCTACTAAAAGCGCCCTATTCGGACTCGCTT<br /> |rRNA<br /> |-<br /> |CTACCTTGTTACGACTTCGTCCCAATCGCCAGTCCCACCTTCGACAGCTCCCT<br /> |rRNA<br /> |-<br /> |AAGGAGGTGATCCAGCCGCACCTTCCGGTACGGCTACCTTGTTACGACTTCGTC<br /> |rRNA<br /> |-<br /> |CCAGGATCAAACTCTCCGTGAATGTATACCCGTAATCGGG<br /> |rRNA<br /> |-<br /> |CTACCTTGTTACGACTTCGTCCCAATCGCCAGTCCCACCTTCG<br /> |rRNA<br /> |-<br /> |CTCTCACTTACCGGCATTCGGAGTTTGGCTAAGGTCAGTAAC<br /> |rRNA<br /> |-<br /> |CATTCGGACACCCCCGGATCAAAGCCTGGTTGACGACTCC<br /> |rRNA<br /> |-<br /> |GCTTATCCCCCACAGTCTCACTGCCGCGCTCTCACTTACC<br /> |rRNA<br /> |-<br /> |CTTAGTCGATGCTCTGGGCTGTTTCCCTCTCGACCATGGAGCTTATC<br /> |rRNA<br /> |-<br /> |CACATCCTTTCCCACTTAGCGTACGCTTAGGGGCCTTAGTCGATGCTCTG<br /> |rRNA<br /> |-<br /> |CTTCTAAGCCAACCTCCTGGTTGTCTGTGCGACTCCACATCCTTTC<br /> |rRNA<br /> |-<br /> |AATCACTAGACCAGTGAGCTATTACGCACTCTTTCAAGGGTGGCTGCTTCTAAGC<br /> |rRNA<br /> |-<br /> |CTACATTGTCGGCGCGGAATCACTAGACCAGTGAGCTATTA<br /> |rRNA<br /> |-<br /> |CGTATTGCTGCAATGACACGACTTCGGCGGTACGCTTGAG<br /> |rRNA<br /> |-<br /> |GCTCCCCTACCCATCACAGCCGGCGTTGGCCGTATTGCTG<br /> |rRNA<br /> |-<br /> |TTCTCACTCGTGAACCGTCCACAACTCGCTTCCGCGGCTGCTTCACCCGGCACACGACG<br /> |rRNA<br /> |-<br /> |GTTTGTATCGCTACTCATGCCTGCATTCTCACTCGTGAACCGTCCACAAC<br /> |rRNA<br /> |-<br /> |CTTAGTCAATCGGCGCAAACGTTTCTCACGTTTGTATCGCTACTCATG<br /> |rRNA<br /> |-<br /> |GACTTACCCTGGGCAGATCAGCTTGACCCAGGAACCCTTAGTCAATC<br /> |rRNA<br /> |-<br /> |TCCACACTGCCTATGTGTTCAGCAGCGGGTGACAGCCCATGACGACTGCCGGGTTT<br /> |rRNA<br /> |-<br /> |TTATCCATCGACTACGCCTGTCGGCCTCGCCTTAGGTCCCGACTTACCCTGGGCA<br /> |rRNA<br /> |-<br /> |GTACCGGAATATCAACCGGTTATCCATCGACTACGCCTGTC<br /> |rRNA<br /> |-<br /> |GGCCTTAGCATCACGATGCTCGATGTTTGACGCTTCACAG<br /> |rRNA<br /> |-<br /> |CTACTAACCGCTTGGTTCGGCGGCTCCACCACTCCCCTCAACTCCGAAGAGATCA<br /> |rRNA<br /> |-<br /> |GATGGACTACCTTCCTGCGTCACCCCATCACTCACCTACTAAC<br /> |rRNA<br /> |-<br /> |CCGGCCTACACCCTTACCCCGGGACAACCACCGCCCGGGATGGACTACC<br /> |rRNA<br /> |-<br /> |CAAGGCATCCACCGTGCGCCCTTAAAAACTTGGCCACAGA<br /> |rRNA<br /> |-<br /> |CAAGGTGTGCGGATTTACCTACACACCGGCCTACACCCTTAC<br /> |rRNA<br /> |-<br /> |CCACAATCGGCTCGGCATCAGGTCTCAGCCACAAGGTGTG<br /> |rRNA<br /> |-<br /> |CTAGAGGCTTTTCTCGACAGCATAGGATCATCCACTTCACCACAATC<br /> |rRNA<br /> |-<br /> |TTTAGGGTACGGGCCGCCATGAAACTCGCTAGAGGCTTTTCTC<br /> |rRNA<br /> |-<br /> |CTCGGTATTCTCTACCTGACCACCTGAGTCGGTTTAGGGTAC<br /> |rRNA<br /> |-<br /> |GGCATTTTGCCGAGTTCCTTAACCATAGTTCACCCGAACG<br /> |rRNA<br /> |-<br /> |CAGCTCAGAGTGCAAAACTCGTCACCAGGTGTGGCCCCCCTTCTCCCGAAGTTA<br /> |rRNA<br /> |-<br /> |TTGTTTTCTCTTCCTGCGGGTACTGAGATGTTTCACTTCCCCGCGTTCCCTCCACA<br /> |rRNA<br /> |-<br /> |TGTGTTTTTAGTAAACAGTCGCTTCTCGCTGGTCTCTGCGGCCACCCCCAGCTCAGA<br /> |rRNA<br /> |-<br /> |TATACATCGCCTTACGGCTTCGCACGGACCTGTGTTTTTAGTAAACAGTCGCTTCT<br /> |rRNA<br /> |-<br /> |GCAGGCGTCAGTCCGTATACATCGCCTTACGGCTTCGCAC<br /> |rRNA<br /> |-<br /> |GAAATGTGACTAACCGGTCCCCTTAACGTTCCAGCACCGGGCAGGC<br /> |rRNA<br /> |-<br /> |GTTTACTGGCGCTTAAGTTCTCAGCTTCGCCACACCGAAATGTGACTAAC<br /> |rRNA<br /> |-<br /> |GACAAGGAATTTCGCTACCTTAGGATGGTTATAGTTACCACCGCCGTTTA<br /> |rRNA<br /> |-<br /> |AAGTCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTA<br /> |rRNA<br /> |-<br /> |CTATGGTTGAGACAGTCGAGAAGTCGTTACGCCATTCGTG<br /> |rRNA<br /> |-<br /> |CTGCGCGAAACGAGCATCTTTACTCGTAGTGCAATTTCAC<br /> |rRNA<br /> |-<br /> |CAATATCAAACTGTAGTAAAGGTCCCGGGGTCTTTCCGTCCTGCTGCGCGAAA<br /> |rRNA<br /> |-<br /> |CAGCTATCCTACACAAGCCGAACCGAACACCAATATCAAACTGTAGTAAA<br /> |rRNA<br /> |-<br /> |CACACTGGCGTGCGAGCTTCAAAGTCTCCCAGCTATCCTACACAAG<br /> |rRNA<br /> |-<br /> |TTAGACATCCAGCACGACCAGACTGGTATTTCAACGACGACTCCACCCACACTGGCGTG<br /> |rRNA<br /> |-<br /> |GTTTCGCTCGCCACTACTCCCGGAATCACGGTTGTTTTCTCTTC<br /> |rRNA<br /> |-<br /> |GATCACGGACCCAGGTTAGACATCCAGCACGACCAGACTG<br /> |rRNA<br /> |-<br /> |CAGTTAAACTACCCATCAGACACTGTCCCTGATCCGGATCAC<br /> |rRNA<br /> |-<br /> |GAACCTTTGGGCGCCTCCGTTACTCTTTAGGAGGCAACCG<br /> |rRNA<br /> |-<br /> |CTTGTGCACTTACACTCAACACCTGATTGCCAACCAGGCTGAGGGAACCTTT<br /> |rRNA<br /> |-<br /> |GACCCGTCGGTCTCACAGTCAAGCTCCCTTGTGCACTTAC<br /> |rRNA<br /> |-<br /> |CTTCCACAAGCCACCGCCGGATCACTAGTCCCGACTTTCGTCCCTG<br /> |rRNA<br /> |-<br /> |GTACCTTTTATCCGTTGAGCGACGGCGCTTCCACAAGCCAC<br /> |rRNA<br /> |-<br /> |CAAACCATCCCGTCGATATGGACTCTTGGGGAAGATCAGCCTGTTATC<br /> |rRNA<br /> |-<br /> |CCAGGATGCGACGAGCCGACATCGAGGTGCCAAACCATCC<br /> |rRNA<br /> |-<br /> |GTCTCACACGTATACGGTTTGGCCTCATCCGGTTTCGCTC<br /> |rRNA<br /> |-<br /> |CTTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTCCAG<br /> |rRNA<br /> |-<br /> |GAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCGCTTTAATG<br /> |rRNA<br /> |-<br /> |CTTCTCAAGACTCCTACGCGCACAGCGGATAGGGACCGAAC<br /> |rRNA<br /> |-<br /> |CACACCAGAGGTTCGTCCGTCCCGGTCCTCTCGTACTAGG<br /> |rRNA<br /> |-<br /> |TTAGATGCTTTCAGCGGTTATCCCTCCCGAACGTAGCCAACCAGCCATGCCCTTGGCA<br /> |rRNA<br /> |-<br /> |CTCATCTCGAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATC<br /> |rRNA<br /> |-<br /> |CAGTCGTCTACTGGGAGCCTTAACCCCTCAAGGGGGTGGGAGTCCTCATCTCGAA<br /> |rRNA<br /> |-<br /> |CTTCCAGATCCGGCCTATCAACCCAGTCGTCTACTGGGAG<br /> |rRNA<br /> |-<br /> |CAGACCGTAAAAGAGAGATCCCACAACCCCGCACACGCAACCCCTGCCGGGTCTCA<br /> |rRNA<br /> |-<br /> |TACATCAACGGTTTCTGACTCGTCTCACGGCCGGCAGACCGTAAAAGAGAGAT<br /> |rRNA<br /> |-<br /> |CTTTCGCATGTCCTTCGCCTACATCAACGGTTTCTGACTC<br /> |rRNA<br /> |-<br /> |ACTACGGGGGTCTTACCCTCTACGCCGGACCTTTCGCATG<br /> |rRNA<br /> |-<br /> |CTACTTGGGTGTCTCTCAAACGAGCCGTTGACGTTTCGACTAC<br /> |rRNA<br /> |-<br /> |GCCAGATTCACACGGGATTTCTCGGGCCCCGTGCTACTTG<br /> |rRNA<br /> |-<br /> |TATCCGCTATCGGTCACCAGGGAATATTTAGGCTTAGCGGGTGGTCCCGCCAGATT<br /> |rRNA<br /> |-<br /> |GTACTTTTCACCATTCCCTCACGGTACTATCCGCTATCGGTCAC<br /> |rRNA<br /> |-<br /> |CTTGTAGGCACACGGTTTCAGGTACTATTTCACTCCGCTCCC<br /> |rRNA<br /> |-<br /> |GGGCCTATCGTGGCCTCCCACGTCCTTCATCGGTTCCTGG<br /> |rRNA<br /> |-<br /> |CACGCAGTCACGAGGATGGAGCAAGCTCCATCCCGACGCTCCCACGGCTTGTAGGCA<br /> |rRNA<br /> |-<br /> |CATTCTTCAAAAGGCACGCAGTCACGAGGATGGAGCAAGC<br /> |rRNA<br /> |-<br /> |TACGGCTTCCCCACTCGGGTTAACCTCGCAACACACCGCAAACTCGCAGGCTCAT<br /> |rRNA<br /> |-<br /> |TCGGACTCGCTTTCGCTACGGCTTCCCCACTCGGGTTAAC<br /> |rRNA<br /> |-<br /> |ATCACTCCGCTTCGGGTCTTGAGCGTGCTACTAAAAACGC<br /> |rRNA<br /> |-<br /> |CTCTTACAGCCGCTTCAACCTGCCCATGGCTAGATCACTC<br /> |rRNA<br /> |-<br /> |GTTTTCAACCCTGGTGGGTTCGGTCCTCCACGACCTCTTAC<br /> |rRNA<br /> |-<br /> |GTTTGATTGGCCTTTCACCCCTAACCACAGGTCATCCCCCAGGTTTTCAACC<br /> |rRNA<br /> |-<br /> |GGAGAACCAGCTATCACGGAGTTTGATTGGCCTTTCACCC<br /> |rRNA<br /> |-<br /> |TATCCAGTGCTCTACCTCCGGCAAGAAACACACGACGCTGCACCTAAATGCATTT<br /> |rRNA<br /> |-<br /> |GAGTTTGGCTAAGGTCAGTAACCCGGTAGGGCCCATCGCCTATCCAGTGCTCTA<br /> |rRNA<br /> |-<br /> |CTGTAAGGCTTAGCTTCCGGGTTCGAAATGTAACCGGGCGTTTCCCCTAC<br /> |rRNA<br /> |-<br /> |GTTCGGCGGCGTCCTACTCTCCCACAGGGTCCCCCCTGCAGTACCATCGGCGCTGTA<br /> |rRNA<br /> |-<br /> |GTATTAGACCCCGTTTCCAGGGCTTGTCCCAGAGTGAAGGGCAGATT<br /> |rRNA<br /> |-<br /> |GAGCTTTCGAACCTCGCAGATGCCTGCGAGGGTCAGTATCCGGTATTAGACCCCGTT<br /> |rRNA<br /> |-<br /> |CATCCTTCACCGCCGGAGCTTTCGAACCTCGCAGATGCCT<br /> |rRNA<br /> |-<br /> |CACCGAAGTGGTTCATCGTTCGACTTGCATGTGTTAAGCAC<br /> |rRNA<br /> |-<br /> |TTCGACTTGCATGTGTTAAGCACGCCGCCAGCGTTCGTCCTGAGCCAGGATCAAACTCT<br /> |rRNA<br /> |-<br /> |CAGATTGCCCACGTGTTACTCACCCGTTCGCCACTAATCC<br /> |rRNA<br /> |-<br /> |GCGGATTTACCTACCTGACGTCCTACACCCTTACCCCGGGACAACCACCGC<br /> |rRNA<br /> |-<br /> |CATCAGGTCTCAGACTCATGTCAGGCGGATTTACCTACCTGAC<br /> |rRNA<br /> |-<br /> |CTATTAGTACCGGTCACCTCCACACCTCACGGTGCTTCCAGATCCGGCCTATCAACC<br /> |rRNA<br /> |-<br /> |GGACAAGCCCTCGGCCTATTAGTACCGGTCACCTCCACAC<br /> |rRNA<br /> |-<br /> |CCGGGTTAACCTCGCAACACACCGCAAACTCGCAGGCTCATTCTTCAAAAGGCACGC<br /> |rRNA<br /> |-<br /> |GGTCTTGAGCGTGCTACTAAAACGCCCTATTCGGACTCGC<br /> |rRNA<br /> |-<br /> |TTCGGCGGCGTCCTACTCTCCCACAGGGTCCCCCCTGCAGTACCATCGGCGCTGTAA<br /> |rRNA<br /> |-<br /> |CCGAAGCCTCCGGCGGTCTGCTTTCTGTGGCACTGTCCCG<br /> |SCOs01<br /> |-<br /> |CTGGGCACTGGTGGTCTCTTACACCACCGTTTCACCCTTAC<br /> |SCOs01<br /> |-<br /> |TTCCTTCTTGACCTTGCTCCGGGTGGGGTTTACCTAGCTGCCCAGGTCGCCCTG<br /> |SCOs01<br /> |-<br /> |CTCGAACGTCCGCGCAGAAGCACCGGGGTGCTTCCTTCTTGACCTTGCTC<br /> |SCOs01<br /> |-<br /> |CTCCAGCGGTCTACCCGCGGACTCGGGCGGGCAGCCCTCGAACGTCCGCGCAGAAG<br /> |SCOs01<br /> |-<br /> |CATCCATCTAGGACCGGCGTTGCCACCGGCCTCCAGCGGTCTAC<br /> |SCOs01<br /> |-<br /> |CTGTGGAGCCCGGACGTTCCTCGGGAAGTCCCGTGAGGGACTC<br /> |SCOs01<br /> |-<br /> |GTTCTGTTCCGACGGGGCCTCGCGGTCCCGTCGGCGACGGCCATCCAT<br /> |SCOs01<br /> |-<br /> |CAGACGAGTCGGGCTGTACGCCGGGTTCTGTTCCGACGGG<br /> |SCOs01<br /> |-<br /> |GTCTGCTTTCTGTGGCACTGTCCCGCGGGTCACCCCGGGTGGCCGTTAGCCATCAC<br /> |SCOs01<br /> |-<br /> |CTTAGTAGGAGGCTCGCTTCGCTACCTGAGATCAGGCAGCGAGGGCGAAGG<br /> |SCOs02<br /> |-<br /> |GAACAGCCCCGGGCTGACACTCCCTTAGTAGGAGGCTCGCTTC<br /> |SCOs02<br /> |-<br /> |GATCAAGGTTTAGTCCCCTAGCTGATGCCAGGATCCGGGTC<br /> |SCOs02<br /> |-<br /> |CAGTCACTGTTTGGTTTCCCTCTTCACCCCGTGACCGGGATCAA<br /> |SCOs02<br /> |-<br /> |GAGATCACGCGGACAAGTCTCCGACGGGCCCAGTCACTGT<br /> |SCOs02<br /> |-<br /> |CTTCCCCTGCTTCGACAGCCGCTGTCGAAACCGATCATCC<br /> |SCOs02<br /> |-<br /> |AATCAGGGCTTCTCCGTGTGCAGTCCGCTTCGATTTTCTCGGCCCCGGAGATCACG<br /> |SCOs02<br /> |-<br /> |GAATCGAACCCGCGTCCAACGGTGCGGAATCAGGGCTTCTC<br /> |SCOs02<br /> |-<br /> |TTTTTCGGCCTGTGGTTTACGAGATCATGGCCGCTTCCTC<br /> |SCOs02<br /> |-<br /> |CTGCTGGGAGGAATCGCGCTTGGTGTTGGCGATTATTTTT<br /> |SCOs02<br /> |-<br /> |CACGCACCCGCCAGAGCCGACCTACCCTTGCTGCCTTCCG<br /> |SCOs03<br /> |}</div> RTEvans https://actinobase.org/index.php?title=RRNA_depletion_with_Streptomyces_coelicolor_A3(2)_specific_probes&diff=2189 RRNA depletion with Streptomyces coelicolor A3(2) specific probes 2023-11-01T16:39:46Z <p>RTEvans: /* Materials */</p> <hr /> <div>==Organisms==<br /> <br /> This protocol has been tested with &lt;em&gt;[[Streptomyces coelicolor]]&lt;/em&gt; A3(2) MT1110<br /> <br /> ==Description==<br /> <br /> rRNA depletion using commercial kits targeting generic bacterial sequences is not 100% efficient in the High GC organism S. coelicolor. <br /> <br /> Depletion using the NEBNext RNA Depletion Core Reagent Set with RNA Sample Purification Beads (https://www.neb.com/en-gb/products/e7870-nebnext-rna-depletion-core-reagent-set-with-rna-sample-purification-beads#Product%20Information) and custom DNA probes designed for &lt;em&gt;S. coelicolor&lt;/em&gt; is more effective than the QIAseq FastSelect 5S/16S/23S kit (Qiagen #335927); NEBNext rRNA Depletion Kit (Bacteria) (NEB #E7850); Illumina Ribo-zero rRNA Removal Kit (Bacteria) (Illumina #MRZMB12424, discontinued). The Illumina kit tested was subsequently replaced by the Illumina Ribo-Zero Plus rRNA Depletion Kit (Illumina #20040526). Additionally three abundant non-mRNA RNAs can be depleted (Table 1).<br /> <br /> '''Table 1.''' Abundant non-mRNA RNAs in &lt;em&gt;S. coelicolor&lt;/em&gt;.<br /> <br /> [[File: rRNA_depletion_table_1.png]]<br /> <br /> ==Materials==<br /> <br /> Single stranded DNA probes (Table 2) at 2µM concentration in IDTE pH 7.5 (10mM Tris-HCl / 0.1mM EDTA). Integrated DNA Technologies can produce these from their 25nmole DNA oligo pool product. Probes are listed in Table 2 in the Notes section below.<br /> <br /> NEBNext RNA Depletion Core Reagent Set with RNA Sample Purification Beads (https://www.neb.com/en-gb/products/e7870-nebnext-rna-depletion-core-reagent-set-with-rna-sample-purification-beads#Product%20Information).<br /> <br /> ==Protocols==<br /> <br /> As per manufacturer’s instructions.<br /> <br /> ==Notes==<br /> <br /> The following histograms illustrate effectiveness of rRNA depletion using various methods. Histograms show percentage of bases aligned to NCBI reference sequence NC_003888.3 using Hisat2 program classified as follows - Coding = RNA aligned to coding sequences; Transcribed not translated = RNA aligned to rRNA, tRNA and sRNA; Intergenic = RNA aligned to 5' or 3' UTRs or between annotated sequences.<br /> <br /> '''QIAseq FastSelect 5S/16S/23S kit'''<br /> <br /> [[File: PanelA.png]] <br /> <br /> '''NEBNext rRNA Depletion Kit (Bacteria)'''<br /> <br /> [[File: PanelB.png]]<br /> <br /> '''Illumina Ribo-zero rRNA Removal Kit (Bacteria)'''<br /> <br /> [[File: PanelC.png]] <br /> <br /> '''rRNA depletion with &lt;em&gt;S. coelicolor&lt;/em&gt; specific probes<br /> <br /> [[File: PanelD.png]]<br /> <br /> <br /> '''Table 2''' List of probes<br /> <br /> {| class=&quot;wikitable&quot;<br /> !Sequence<br /> !Target<br /> |-<br /> |CAAGTCCTTGCGCGACGCTCCCACGGCTTGTAGGCACACGGTTTCAGGTACTATTT<br /> |rRNA<br /> |-<br /> |CAGTCACGACGCAAGGACAAGTCCTTGCGCGACGCTCCCAC<br /> |rRNA<br /> |-<br /> |ACCTCGCAACACACCGCAAACTCGCAGGCTCATTCTTCAAAAGGCACGCAGTCACGACG<br /> |rRNA<br /> |-<br /> |CTACGGCTTCCCCACCCGGGTTAACCTCGCAACACACCGC<br /> |rRNA<br /> |-<br /> |TTAACCCAACATCTCACGACACGAGCTGACGACAGCCATGCACCACCTGTACACCGA<br /> |rRNA<br /> |-<br /> |CGCTCGTTGCGGGACTTAACCCAACATCTCACGACACGAG<br /> |rRNA<br /> |-<br /> |CAACACCCCGAAGGGCTTGCTGGCAACACGGGACAAGGGTTG<br /> |rRNA<br /> |-<br /> |CACCTTCCTCCGAGTTGACCCCGGCGGTCTCCCGTGAGTC<br /> |rRNA<br /> |-<br /> |GACATAAGGGGCATGATGACTTGACGTCGTCCCCACCTTC<br /> |rRNA<br /> |-<br /> |GTATCGCAGCTCATTGTACCGGCCATTGTAGCACGTGTGCAGCCCAAGACATAA<br /> |rRNA<br /> |-<br /> |TTTTGAGATTCGCTCCACCTTGCGGTATCGCAGCTCATTGTAC<br /> |rRNA<br /> |-<br /> |CAGACCCCAATCCGAACTGAGACCGGCTTTTTGAGATTCG<br /> |rRNA<br /> |-<br /> |AATGCTGATCTGCGATTACTAGCGACTCCGACTTCATGGGGTCGAGTTGCAGAC<br /> |rRNA<br /> |-<br /> |GAACGTATTCACCGCAGCAATGCTGATCTGCGATTACTAG<br /> |rRNA<br /> |-<br /> |TACCGACTTTCGTGACGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTC<br /> |rRNA<br /> |-<br /> |GCCACCGGCTTCGGGTGTTACCGACTTTCGTGACGTGACG<br /> |rRNA<br /> |-<br /> |TTCGTCCCAATCGCCAGTCCCACCTTCGACAGCTCCCTCCCACAAGGGGTTGGG<br /> |rRNA<br /> |-<br /> |CGCACCTTCCGGTACGGCTACCTTGTTACGACTTCGTCCCAATCGCCAGTCCCA<br /> |rRNA<br /> |-<br /> |TTCGACCCTCGCAGATGCCTGCGAGGGTCAGTATCCGGTATTAGACCCCGTTTCCAG<br /> |rRNA<br /> |-<br /> |GAAAGGAGGTGATCCAGCCGCACCTTCCGGTACGGCTACC<br /> |rRNA<br /> |-<br /> |TTCACCGCCGGAGCTTTCGACCCTCGCAGATGCCTGCGAG<br /> |rRNA<br /> |-<br /> |CATTACCTCACCAACAAGCTGATAGGCCGCGGGCTCATCCTTC<br /> |rRNA<br /> |-<br /> |CTCTCAGGCCGGCTACCCGTCGTCGCCTTGGTGAGCCATTAC<br /> |rRNA<br /> |-<br /> |GTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGGCCGGTCG<br /> |rRNA<br /> |-<br /> |TGCATCAGGCTTTCGCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCT<br /> |rRNA<br /> |-<br /> |TGCTGAAAGAGGTTTACAACCCGAAGGCCGTCATCCCTCACGCGGCGTCGCTGCATCA<br /> |rRNA<br /> |-<br /> |TTCTTCTGCAGGTACCGTCACTTTCGCTTCTTCCCTGCTGAAAGAGGTTTACAA<br /> |rRNA<br /> |-<br /> |CTACGTATTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTCTG<br /> |rRNA<br /> |-<br /> |CTCTTTACGCCCAATAATTCCGGACAACGCTTGCGCCCTACGTATTAC<br /> |rRNA<br /> |-<br /> |GGGCTTTCACAACCGACGTGACAAGCCGCCTACGAGCTCT<br /> |rRNA<br /> |-<br /> |CTACCGAACTCTAGCCTGCCCGTATCGACTGCAGACCCGGGGTTAAGCCCCG<br /> |rRNA<br /> |-<br /> |ATATCTGCGCATTTCACCGCTACACCAGGAATTCCGATCTCCCCTACCGAACTCTAG<br /> |rRNA<br /> |-<br /> |CGGTGTTCCTCCTGATATCTGCGCATTTCACCGCTACACC<br /> |rRNA<br /> |-<br /> |ACGCTTTCGCTCCTCAGCGTCAGTATCGGCCCAGAGATCCGCCTTCGCCACCGGTGT<br /> |rRNA<br /> |-<br /> |GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCG<br /> |rRNA<br /> |-<br /> |ACAACGTGGAATGTTGCCCACACCTAGTGCCCACCGTTTACGGCGTGGACTACCA<br /> |rRNA<br /> |-<br /> |CTTAATGCGTTAGCTGCGGCACGGACAACGTGGAATGTTG<br /> |rRNA<br /> |-<br /> |TCAATTCCTTTGAGTTTTAGCCTTGCGGCCGTACTCCCCAGGCGGGGCACTTAA<br /> |rRNA<br /> |-<br /> |ATTAAGCCACATGCTCCGCCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTTA<br /> |rRNA<br /> |-<br /> |TCAAGCCTTGGTAAGGTTCTTCGCGTTGCGTCGAATTAAGCCACATGCTCCGCCGCTT<br /> |rRNA<br /> |-<br /> |CATCTCTGATGCTTTCCGGTGTATGTCAAGCCTTGGTAAGGTTCTTC<br /> |rRNA<br /> |-<br /> |ACAGCCATGCACCACCTGTACACCGACCACAAGGGGGGCACCATCTCTGATGCTTTCC<br /> |rRNA<br /> |-<br /> |TGTAACCGGGCGTTTCCCCTACGCTATGACCACCGAAACC<br /> |rRNA<br /> |-<br /> |CAGTACCATCGGCGCTGTAAGGCTTAGCTTCCGGGTTCGAAATGTAAC<br /> |rRNA<br /> |-<br /> |GTTCGGCGGCGTCCTACTCTCCCACAGGGTCCCCCCTGCA<br /> |rRNA<br /> |-<br /> |CACTCCCCTCAACTCCGAAGAGATCAGGGCGGCTTCACGG<br /> |rRNA<br /> |-<br /> |CCATCACTCACCTACTAACCGCTTGGTTCGGCGGCTCCAC<br /> |rRNA<br /> |-<br /> |GTCCTTCATCGGTTCCTGGTGCCAAGGCATCCACCGTGCGCCCTTAAAAACTT<br /> |rRNA<br /> |-<br /> |CTTGTAGGCACACGGTTTCAGGTACTATTTCACTCCGCTCCCG<br /> |rRNA<br /> |-<br /> |CACGCAGTCACGAGACACCAAGCAAGCTTGATGTCCGACGCTCCCACGGCTTGTAGGCAC<br /> |rRNA<br /> |-<br /> |TCTTCAAAAGGCACGCAGTCACGAGACACCAAGCAAGCTT<br /> |rRNA<br /> |-<br /> |CACCCGGGTTAACCTCGCAACACACTGCAAACTCGCAGGC<br /> |rRNA<br /> |-<br /> |GCGTGCTACTAAAACGCCCTATTCGGACTCGCTTTCGCTACGGCTTCC<br /> |rRNA<br /> |-<br /> |GATCACTCCGCTTCGGGTCTTGAGCGTGCTACTAAAACGC<br /> |rRNA<br /> |-<br /> |TCGCAGATGCCTGCAAGGGTCAGTATCCGGTATTAGACCCCGTTT<br /> |rRNA<br /> |-<br /> |TCATCCTTCACCGCCGCAGCTTTCGAACCTCGCAGATGCC<br /> |rRNA<br /> |-<br /> |ATGTAACCGGGCGTTTCCCCTACGCTATGACCACCGAAAC<br /> |rRNA<br /> |-<br /> |CGTCCTACTCTCCCACAGGGTCCCCCCTGCAGTACCATCG<br /> |rRNA<br /> |-<br /> |CATCGCCTATCCAGTGCTCTACCTCCGGCAAGAAACACAC<br /> |rRNA<br /> |-<br /> |CATTCGGAGTTTGGCTAAGGTCAGTAACCCGGTAGGGCCCATCG<br /> |rRNA<br /> |-<br /> |CAGTCTCACTGCCGCGCTCTCACTTACCGGCATTCGGAGTTTG<br /> |rRNA<br /> |-<br /> |ATGCTCTGGGCTGTTTCCCTCTCGACCATGGAGCTTATCCCCCACAGTCTCACTG<br /> |rRNA<br /> |-<br /> |CTTAGGGGCCTTAGTCGATGCTCTGGGCTGTTTCCCTCTCG<br /> |rRNA<br /> |-<br /> |GTTGTCTGTGCGACTCCACATCCTTTCCCACTTAGCGTACGCTTAG<br /> |rRNA<br /> |-<br /> |TATTACGCACTCTTTCAAGGGTGGCTGCTTCTAAGCCAACCTCCTGGTTGTCTGT<br /> |rRNA<br /> |-<br /> |GATCAAAGCCTGGTTGACGACTCCCCGGGGCCTATCGTGG<br /> |rRNA<br /> |-<br /> |CGGAATCACTAGACCAGTGAGCTATTACGCACTCTTTCAAGG<br /> |rRNA<br /> |-<br /> |TATTGCTGCAATGACACGACTTCGGCGGTACGCTTGAGCCCCGCTACATTGTCG<br /> |rRNA<br /> |-<br /> |CATCACAGCCGGCGTTGGCCGTATTGCTGCAATGACACGACTTC<br /> |rRNA<br /> |-<br /> |TCCACAACTCGCTTCCGCGGCTGCTTCACCCGGCACACGACGCTCCCCTACCCATCACA<br /> |rRNA<br /> |-<br /> |TACTCATGCCTGCATTCTCACTCGTGAACCGTCCACAACTCGCTTCCGCGGCTGCTT<br /> |rRNA<br /> |-<br /> |CAAACGTTTCTCACGTTTGTATCGCTACTCATGCCTGCATTCTCACTC<br /> |rRNA<br /> |-<br /> |CAGGAACCCTTAGTCAATCGGCGCAAACGTTTCTCACGTTT<br /> |rRNA<br /> |-<br /> |CTTAGGTCCCGACTTACCCTGGGCAGATCAGCTTGACCCAG<br /> |rRNA<br /> |-<br /> |GAATATCAACCGGTTATCCATCGACTACGCCTGTCGGCCTCGCCTTAGGTC<br /> |rRNA<br /> |-<br /> |TTAGCATCACGATGCTCGATGTTTGACGCTTCACAGCGGGTACCGGAATATCAA<br /> |rRNA<br /> |-<br /> |GAAGAGATCAGGGCGGCTTCACGGCCTTAGCATCACGATG<br /> |rRNA<br /> |-<br /> |CATCACTCACCTACTAACCGCTTGGTTCGGCGGCTCCACC<br /> |rRNA<br /> |-<br /> |TGTGTTCAGCAGCGGGTGACAGCCCATGACGACTGCCGGGTTTCCCCATTCGGACACCC<br /> |rRNA<br /> |-<br /> |GACAACCACCGCCCGGGATGGACTACCTTCCTGCGTCACCCCAT<br /> |rRNA<br /> |-<br /> |CAGATGCTCGCGTCCACTGTGCAGTTCTCAAACAACGACC<br /> |rRNA<br /> |-<br /> |TGCGGATTTACCTACACACCGGCCTACACCCTTACCCCGGGACAACCACCG<br /> |rRNA<br /> |-<br /> |CATCAGGTCTCAGCCACAAGGTGTGCGGATTTACCTACAC<br /> |rRNA<br /> |-<br /> |TTTTCTCGACAGCATAGGATCATCCACTTCACCACAATCGGCTCGGCATCAGGTCT<br /> |rRNA<br /> |-<br /> |CCATGAAACTCGCTAGAGGCTTTTCTCGACAGCATAGGATCATC<br /> |rRNA<br /> |-<br /> |TATTCTCTACCTGACCACCTGAGTCGGTTTAGGGTACGGGCCGCCATGAAACT<br /> |rRNA<br /> |-<br /> |GAGTTCCTTAACCATAGTTCACCCGAACGCCTCGGTATTCTCTACCTGAC<br /> |rRNA<br /> |-<br /> |CTTCTCCCGAAGTTACGGGGGCATTTTGCCGAGTTCCTTAACCA<br /> |rRNA<br /> |-<br /> |CACCCCCAGCTCAGAGTGCAAAACTCGTCACCAGGTGTGG<br /> |rRNA<br /> |-<br /> |GTGTTTTTAGTAAACAGTCGCTTCTCGCTGGTCTCTGCGG<br /> |rRNA<br /> |-<br /> |GTTCCCTCCACACTGCCTATGTGTTCAGCAGCGGGTGACAG<br /> |rRNA<br /> |-<br /> |GTATACATCGCCTTACGGCTTCGCACGGACCTGTGTTTTTA<br /> |rRNA<br /> |-<br /> |CTTAACGTTCCAGCACCGGGCAGGCGTCAGTCCGTATACATC<br /> |rRNA<br /> |-<br /> |TAAGTTCTCAGCTTCGCCACACCGAAATGTGACTAACCGGTCCCCTTAACGTT<br /> |rRNA<br /> |-<br /> |TTAGGATGGTTATAGTTACCACCGCCGTTTACTGGCGCTTAAGTTCTCAG<br /> |rRNA<br /> |-<br /> |GTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGATGGTTATAGT<br /> |rRNA<br /> |-<br /> |TATGGTTGAGACAGTCGAGAAGTCGTTACGCCATTCGTGCAGGTCGGAACTTACC<br /> |rRNA<br /> |-<br /> |GAAACGAGCATCTTTACTCGTAGTGCAATTTCACCGGGCCTATGGTTGAG<br /> |rRNA<br /> |-<br /> |GGTCTTTCCGTCCTGCTGCGCGAAACGAGCATCTTTACTC<br /> |rRNA<br /> |-<br /> |GAACCGAACACCAATATCAAACTGTAGTAAAGGTCCCGGGGTCTTTC<br /> |rRNA<br /> |-<br /> |CTTCAAAGTCTCCCAGCTATCCTACACAAGCCGAACCGAACACCAATA<br /> |rRNA<br /> |-<br /> |AGACTGGTATTTCAACGACGACTCCACCCACACTGGCGTGCGAGCTTCAAAGTCT<br /> |rRNA<br /> |-<br /> |GTTAGACATCCAGCACGACCAGACTGGTATTTCAACGACGACTC<br /> |rRNA<br /> |-<br /> |TTAAACTACCCATCAGACACTGTCCCTGATCCGGATCACGGACCCAGGTTAGACAT<br /> |rRNA<br /> |-<br /> |GAATCACGGTTGTTTTCTCTTCCTGCGGGTACTGAGATGTTTCACTTC<br /> |rRNA<br /> |-<br /> |GTTACTCTTTAGGAGGCAACCGCCCCAGTTAAACTACCCATCAGACACTG<br /> |rRNA<br /> |-<br /> |ACTCAACACCTGATTGCCAACCAGGCTGAGGGAACCTTTGGGCGCCTCCGTTACT<br /> |rRNA<br /> |-<br /> |GTCTCACAGTCAAGCTCCCTTGTGCACTTACACTCAACACCTGATTG<br /> |rRNA<br /> |-<br /> |GACTTTCGTCCCTGCTCGACCCGTCGGTCTCACAGTCAAG<br /> |rRNA<br /> |-<br /> |TACCTTTTATCCGTTGAGCGACGGCGCTTCCACAAGCCACCGCCGGATCACTAGTC<br /> |rRNA<br /> |-<br /> |GTCGATATGGACTCTTGGGGAAGATCAGCCTGTTATCCCCGGGGTACCTTTTAT<br /> |rRNA<br /> |-<br /> |GACATCGAGGTGCCAAACCATCCCGTCGATATGGACTCTTG<br /> |rRNA<br /> |-<br /> |TTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTCCAGCCCCAGGATGCGACGA<br /> |rRNA<br /> |-<br /> |TCACGACGTTCTAAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACC<br /> |rRNA<br /> |-<br /> |GATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTC<br /> |rRNA<br /> |-<br /> |GTCCTCTCGTACTAGGGACAGCCCTTCTCAAGACTCCTAC<br /> |rRNA<br /> |-<br /> |TCTCACACGTATACGGTTTGGCCTCATCCGGTTTCGCTCGCCACTACTCCCGGAAT<br /> |rRNA<br /> |-<br /> |CATGCCCTTGGCAGGACAACTGGCACACCAGAGGTTCGTC<br /> |rRNA<br /> |-<br /> |CTTAGATGCTTTCAGCGGTTATCCCTCCCGAACGTAGCCAAC<br /> |rRNA<br /> |-<br /> |GTGGGAGTCCTCATCTCGAAGCAGGCTTCCCGCTTAGATG<br /> |rRNA<br /> |-<br /> |CTATCAACCCAGTCGTCTACTGGGAGCCTTAACCCCTCAAGG<br /> |rRNA<br /> |-<br /> |GCCTATTAGTACCGGTCACCTCCACACCTCACGGTGCTTCCAGATCCGGCCTATCAAC<br /> |rRNA<br /> |-<br /> |AGGACAAGCCCTCGGCCTATTAGTACCGGTCACCTCCACA<br /> |rRNA<br /> |-<br /> |CACACGCAACCCCTGCCGGGTCTCACACGTATACGGTTTG<br /> |rRNA<br /> |-<br /> |TTTCTGACTCGTCTCACGGCCGGCAGACCGTAAAAGAGAGATCCCACAACCCCGCACA<br /> |rRNA<br /> |-<br /> |TTTCGCATGTCCTTCGCCTACATCAACGGTTTCTGACTCGTCTCACGGCCGGCAGA<br /> |rRNA<br /> |-<br /> |CTTACCCTCTACGCCGGACCTTTCGCATGTCCTTCGCCTACATC<br /> |rRNA<br /> |-<br /> |GTGTCTCTCAAACGAGCCGTTGACGTTTCGACTACGGGGGTCTTACCCTCTA<br /> |rRNA<br /> |-<br /> |ATCCACCGTGCGCCCTTAAAAACTTGGCCACAGATGCTCG<br /> |rRNA<br /> |-<br /> |GATTTCTCGGGCCCCGTGCTACTTGGGTGTCTCTCAAACGAG<br /> |rRNA<br /> |-<br /> |GAATATTTAGGCTTAGCGGGTGGTCCCGCCAGATTCACACGGGATTTCTC<br /> |rRNA<br /> |-<br /> |CTCACGGTACTATCCGCTATCGGTCACCAGGGAATATTTAGGCTTAG<br /> |rRNA<br /> |-<br /> |GTTTCAGGTACTATTTCACTCCGCTCCCGCGGTACTTTTCACCATTC<br /> |rRNA<br /> |-<br /> |GATGGAGCAAGCTCCATCCCGACGCTCCCACGGCTTGTAGGCACACGGTTTCAGGTACT<br /> |rRNA<br /> |-<br /> |CACGCAGTCACGAGGATGGAGCAAGCTCCATCCCGACGCTC<br /> |rRNA<br /> |-<br /> |CTCGGGTTAACCTCGCAACACACCGCAAACTCGCAGGCTCATTCTTCAAAAGGCACGC<br /> |rRNA<br /> |-<br /> |CTACGGCTTCCCCACTCGGGTTAACCTCGCAACACACCGC<br /> |rRNA<br /> |-<br /> |GTCTTGAGCGTGCTACTAAAAACGCCCTATTCGGACTCGC<br /> |rRNA<br /> |-<br /> |CTCCCACGTCCTTCATCGGTTCCTGGTGCCAAGGCATCCAC<br /> |rRNA<br /> |-<br /> |CTTCAACCTGCCCATGGCTAGATCACTCCGCTTCGGGTCTTGAG<br /> |rRNA<br /> |-<br /> |GGTTCGGTCCTCCACGACCTCTTACAGCCGCTTCAACCTG<br /> |rRNA<br /> |-<br /> |CTAACCACAGGTCATCCCCCAGGTTTTCAACCCTGGTGGGTTCGGTCC<br /> |rRNA<br /> |-<br /> |AGAACCAGCTATCACGGAGTTTGATTGGCCTTTCACCCCTAACCACAGGTCATC<br /> |rRNA<br /> |-<br /> |CAAGAAACACACGACGCTGCACCTAAATGCATTTCGGGGAGAACCAGCTATCA<br /> |rRNA<br /> |-<br /> |TTTTAGCCTTGCGGCCGTACTCCCCAGGCGGGGCACTTAATGCGTTAGCTGCGGCAC<br /> |rRNA<br /> |-<br /> |CCGTCAATTCCTTTGAGTTTTAGCCTTGCGGCCGTACTCC<br /> |rRNA<br /> |-<br /> |AATAATCCGACCGTTCACAGCGTCCTCGCTGTTGTGTTACTTCAAAGGAA<br /> |rRNA<br /> |-<br /> |TTCTTCGCGTTGCGTCGAATTAAGCCACATGCTCCGCCGCTTGTGCGGGCCCCCGTCAA<br /> |rRNA<br /> |-<br /> |GTGTATGTCAAGCCTTGGTAAGGTTCTTCGCGTTGCGTCGAATTAAG<br /> |rRNA<br /> |-<br /> |GGGGGCACCATCTCTGATGCTTTCCGGTGTATGTCAAGCC<br /> |rRNA<br /> |-<br /> |TCACGACACGAGCTGACGACAGCCATGCACCACCTGTACACCGACCACAAGGGGGGCACC<br /> |rRNA<br /> |-<br /> |CTTAACCCAACATCTCACGACACGAGCTGACGACAGCCATG<br /> |rRNA<br /> |-<br /> |CAACACGGGACAAGGGTTGCGCTCGTTGCGGGACTTAACC<br /> |rRNA<br /> |-<br /> |GTCTCCCGTGAGTCCCCAACACCCCGAAGGGCTTGCTGGCAAC<br /> |rRNA<br /> |-<br /> |ACATAAGGGGCATGATGACTTGACGTCGTCCCCACCTTCCTCCGAGTTGACCCCG<br /> |rRNA<br /> |-<br /> |ATTGTACCGGCCATTGTAGCACGTGTGCAGCCCAAGACATAAGGGGCATGATGACTTGA<br /> |rRNA<br /> |-<br /> |TTTTGAGATTCGCTCCACCTTGCGGTATCGCAGCTCATTGTACCGGCCATTGTAGCACGT<br /> |rRNA<br /> |-<br /> |CAATCCGAACTGAGACCGGCTTTTTGAGATTCGCTCCACCTTGCGGTAT<br /> |rRNA<br /> |-<br /> |ATTACTAGCGACTCCGACTTCATGGGGTCGAGTTGCAGACCCCAATCCGAACTGAGA<br /> |rRNA<br /> |-<br /> |TATTCACCGCAGCAATGCTGATCTGCGATTACTAGCGACTCCGACTTCATG<br /> |rRNA<br /> |-<br /> |GTGTACAAGGCCCGGGAACGTATTCACCGCAGCAATGCTG<br /> |rRNA<br /> |-<br /> |GTAATCGGGTGACACCACGAGAGCGGAACAGTCGGAGGAATAA<br /> |rRNA<br /> |-<br /> |GTGTTACCGACTTTCGTGACGTGACGGGCGGTGTGTACAAG<br /> |rRNA<br /> |-<br /> |CACCTTCGACAGCTCCCTCCCACAAGGGGTTGGGCCACCGGCTTCGGGTGTTA<br /> |rRNA<br /> |-<br /> |CTACCTTGTTACGACTTCGTCCCAATCGCCAGTCCCACCTTCGACAG<br /> |rRNA<br /> |-<br /> |TCCTTAGAAAGGAGGTGATCCAGCCGCACCTTCCGGTACGGCTACCTTGTTACGA<br /> |rRNA<br /> |-<br /> |TGCAGTCGGCTATGTGCTCCTTAGAAAGGAGGTGATCCAG<br /> |rRNA<br /> |-<br /> |GATCAAACTCTCCGTGAATGTATACCCGTAATCGGGTGAC<br /> |rRNA<br /> |-<br /> |AAGTGGTTCATCGTTCGACTTGCATGTGTTAAGCACGCCGCCAGCGTTCGTCCTGA<br /> |rRNA<br /> |-<br /> |TATCAACATATCTGGCGTTGACTTTTGGCACGCTGTTGAGTTCTCAAGGAA<br /> |rRNA<br /> |-<br /> |TGTTACTCACCCGTTCGCCACTAATCCCCACCGAAGTGGTTCATCGTTCGACTT<br /> |rRNA<br /> |-<br /> |CTTGTCCCAGAGTGAAGGGCAGATTGCCCACGTGTTACTC<br /> |rRNA<br /> |-<br /> |GCAGATGCCTGCGAGGGTCAGTATCCGGTATTAGACCCCGTTTCCAGGGCTTGTCCCA<br /> |rRNA<br /> |-<br /> |ATCCTTCACCGCCGGAGCTTTCGAACCTCGCAGATGCCTGCGAGGGTCAGTATCC<br /> |rRNA<br /> |-<br /> |GATAGGCCGCGGGCTCATCCTTCACCGCCGGAGCTTTCGA<br /> |rRNA<br /> |-<br /> |GTCGCCTTGGTGAGCCATTACCTCACCAACAAGCTGATAG<br /> |rRNA<br /> |-<br /> |TAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGGCCGGTCGCCCTCTCAGGCCGGCTA<br /> |rRNA<br /> |-<br /> |ATTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTC<br /> |rRNA<br /> |-<br /> |CGTCGCTGCATCAGGCTTTCGCCCATTGTGCAATATTCCC<br /> |rRNA<br /> |-<br /> |CTTCTTCCCTGCTGAAAGAGGTTTACAACCCGAAGGCCGTCATC<br /> |rRNA<br /> |-<br /> |TTCAAAGGAACCTCAACCCGAACGGAGATCCGATCAGGTCGGGGTATCAACATAT<br /> |rRNA<br /> |-<br /> |GTAGTTAGCCGGCGCTTCTTCTGCAGGTACCGTCACTTTC<br /> |rRNA<br /> |-<br /> |ATAATTCCGGACAACGCTTGCGCCCTACGTATTACCGCGGCTGCTGGCACGTAGTTA<br /> |rRNA<br /> |-<br /> |CTTTCACAACCGACGTGACAAGCCGCCTACGAGCTCTTTACGCCCAATAATT<br /> |rRNA<br /> |-<br /> |TATCGACTGCAGACCCGGGGTTAAGCCCCGGGCTTTCACAACCGACGTGACAAG<br /> |rRNA<br /> |-<br /> |GATCTCCCCTACCGAACTCTAGCCTGCCCGTATCGACTGCAGAC<br /> |rRNA<br /> |-<br /> |CTGATATCTGCGCATTTCACCGCTACACCAGGAATTCCGATCTC<br /> |rRNA<br /> |-<br /> |CAGAGATCCGCCTTCGCCACCGGTGTTCCTCCTGATATCTG<br /> |rRNA<br /> |-<br /> |CACGCTTTCGCTCCTCAGCGTCAGTATCGGCCCAGAGATC<br /> |rRNA<br /> |-<br /> |CACCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTTC<br /> |rRNA<br /> |-<br /> |ACTTAATGCGTTAGCTGCGGCACGGACAACGTGGAATGTTGCCCACACCTAGTGCCCA<br /> |rRNA<br /> |-<br /> |GTTCGAAATGTAACCGGGCGTTTCCCCTACGCTATGACCACCGAAA<br /> |rRNA<br /> |-<br /> |CTTAGGATGGTTATAGTTACCACCGCCGTTTACTGGCGCTTAAGTTCTCA<br /> |rRNA<br /> |-<br /> |CCAAGCAAGCTTGATGTCCGACGCTCCCACGGCTTGTAGGCACACGGTTTCAGGTACTA<br /> |rRNA<br /> |-<br /> |CAGTCACGAGACACCAAGCAAGCTTGATGTCCGACGCTCC<br /> |rRNA<br /> |-<br /> |CTTGAGCGTGCTACTAAAAGCGCCCTATTCGGACTCGCTT<br /> |rRNA<br /> |-<br /> |CTACCTTGTTACGACTTCGTCCCAATCGCCAGTCCCACCTTCGACAGCTCCCT<br /> |rRNA<br /> |-<br /> |AAGGAGGTGATCCAGCCGCACCTTCCGGTACGGCTACCTTGTTACGACTTCGTC<br /> |rRNA<br /> |-<br /> |CCAGGATCAAACTCTCCGTGAATGTATACCCGTAATCGGG<br /> |rRNA<br /> |-<br /> |CTACCTTGTTACGACTTCGTCCCAATCGCCAGTCCCACCTTCG<br /> |rRNA<br /> |-<br /> |CTCTCACTTACCGGCATTCGGAGTTTGGCTAAGGTCAGTAAC<br /> |rRNA<br /> |-<br /> |CATTCGGACACCCCCGGATCAAAGCCTGGTTGACGACTCC<br /> |rRNA<br /> |-<br /> |GCTTATCCCCCACAGTCTCACTGCCGCGCTCTCACTTACC<br /> |rRNA<br /> |-<br /> |CTTAGTCGATGCTCTGGGCTGTTTCCCTCTCGACCATGGAGCTTATC<br /> |rRNA<br /> |-<br /> |CACATCCTTTCCCACTTAGCGTACGCTTAGGGGCCTTAGTCGATGCTCTG<br /> |rRNA<br /> |-<br /> |CTTCTAAGCCAACCTCCTGGTTGTCTGTGCGACTCCACATCCTTTC<br /> |rRNA<br /> |-<br /> |AATCACTAGACCAGTGAGCTATTACGCACTCTTTCAAGGGTGGCTGCTTCTAAGC<br /> |rRNA<br /> |-<br /> |CTACATTGTCGGCGCGGAATCACTAGACCAGTGAGCTATTA<br /> |rRNA<br /> |-<br /> |CGTATTGCTGCAATGACACGACTTCGGCGGTACGCTTGAG<br /> |rRNA<br /> |-<br /> |GCTCCCCTACCCATCACAGCCGGCGTTGGCCGTATTGCTG<br /> |rRNA<br /> |-<br /> |TTCTCACTCGTGAACCGTCCACAACTCGCTTCCGCGGCTGCTTCACCCGGCACACGACG<br /> |rRNA<br /> |-<br /> |GTTTGTATCGCTACTCATGCCTGCATTCTCACTCGTGAACCGTCCACAAC<br /> |rRNA<br /> |-<br /> |CTTAGTCAATCGGCGCAAACGTTTCTCACGTTTGTATCGCTACTCATG<br /> |rRNA<br /> |-<br /> |GACTTACCCTGGGCAGATCAGCTTGACCCAGGAACCCTTAGTCAATC<br /> |rRNA<br /> |-<br /> |TCCACACTGCCTATGTGTTCAGCAGCGGGTGACAGCCCATGACGACTGCCGGGTTT<br /> |rRNA<br /> |-<br /> |TTATCCATCGACTACGCCTGTCGGCCTCGCCTTAGGTCCCGACTTACCCTGGGCA<br /> |rRNA<br /> |-<br /> |GTACCGGAATATCAACCGGTTATCCATCGACTACGCCTGTC<br /> |rRNA<br /> |-<br /> |GGCCTTAGCATCACGATGCTCGATGTTTGACGCTTCACAG<br /> |rRNA<br /> |-<br /> |CTACTAACCGCTTGGTTCGGCGGCTCCACCACTCCCCTCAACTCCGAAGAGATCA<br /> |rRNA<br /> |-<br /> |GATGGACTACCTTCCTGCGTCACCCCATCACTCACCTACTAAC<br /> |rRNA<br /> |-<br /> |CCGGCCTACACCCTTACCCCGGGACAACCACCGCCCGGGATGGACTACC<br /> |rRNA<br /> |-<br /> |CAAGGCATCCACCGTGCGCCCTTAAAAACTTGGCCACAGA<br /> |rRNA<br /> |-<br /> |CAAGGTGTGCGGATTTACCTACACACCGGCCTACACCCTTAC<br /> |rRNA<br /> |-<br /> |CCACAATCGGCTCGGCATCAGGTCTCAGCCACAAGGTGTG<br /> |rRNA<br /> |-<br /> |CTAGAGGCTTTTCTCGACAGCATAGGATCATCCACTTCACCACAATC<br /> |rRNA<br /> |-<br /> |TTTAGGGTACGGGCCGCCATGAAACTCGCTAGAGGCTTTTCTC<br /> |rRNA<br /> |-<br /> |CTCGGTATTCTCTACCTGACCACCTGAGTCGGTTTAGGGTAC<br /> |rRNA<br /> |-<br /> |GGCATTTTGCCGAGTTCCTTAACCATAGTTCACCCGAACG<br /> |rRNA<br /> |-<br /> |CAGCTCAGAGTGCAAAACTCGTCACCAGGTGTGGCCCCCCTTCTCCCGAAGTTA<br /> |rRNA<br /> |-<br /> |TTGTTTTCTCTTCCTGCGGGTACTGAGATGTTTCACTTCCCCGCGTTCCCTCCACA<br /> |rRNA<br /> |-<br /> |TGTGTTTTTAGTAAACAGTCGCTTCTCGCTGGTCTCTGCGGCCACCCCCAGCTCAGA<br /> |rRNA<br /> |-<br /> |TATACATCGCCTTACGGCTTCGCACGGACCTGTGTTTTTAGTAAACAGTCGCTTCT<br /> |rRNA<br /> |-<br /> |GCAGGCGTCAGTCCGTATACATCGCCTTACGGCTTCGCAC<br /> |rRNA<br /> |-<br /> |GAAATGTGACTAACCGGTCCCCTTAACGTTCCAGCACCGGGCAGGC<br /> |rRNA<br /> |-<br /> |GTTTACTGGCGCTTAAGTTCTCAGCTTCGCCACACCGAAATGTGACTAAC<br /> |rRNA<br /> |-<br /> |GACAAGGAATTTCGCTACCTTAGGATGGTTATAGTTACCACCGCCGTTTA<br /> |rRNA<br /> |-<br /> |AAGTCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTA<br /> |rRNA<br /> |-<br /> |CTATGGTTGAGACAGTCGAGAAGTCGTTACGCCATTCGTG<br /> |rRNA<br /> |-<br /> |CTGCGCGAAACGAGCATCTTTACTCGTAGTGCAATTTCAC<br /> |rRNA<br /> |-<br /> |CAATATCAAACTGTAGTAAAGGTCCCGGGGTCTTTCCGTCCTGCTGCGCGAAA<br /> |rRNA<br /> |-<br /> |CAGCTATCCTACACAAGCCGAACCGAACACCAATATCAAACTGTAGTAAA<br /> |rRNA<br /> |-<br /> |CACACTGGCGTGCGAGCTTCAAAGTCTCCCAGCTATCCTACACAAG<br /> |rRNA<br /> |-<br /> |TTAGACATCCAGCACGACCAGACTGGTATTTCAACGACGACTCCACCCACACTGGCGTG<br /> |rRNA<br /> |-<br /> |GTTTCGCTCGCCACTACTCCCGGAATCACGGTTGTTTTCTCTTC<br /> |rRNA<br /> |-<br /> |GATCACGGACCCAGGTTAGACATCCAGCACGACCAGACTG<br /> |rRNA<br /> |-<br /> |CAGTTAAACTACCCATCAGACACTGTCCCTGATCCGGATCAC<br /> |rRNA<br /> |-<br /> |GAACCTTTGGGCGCCTCCGTTACTCTTTAGGAGGCAACCG<br /> |rRNA<br /> |-<br /> |CTTGTGCACTTACACTCAACACCTGATTGCCAACCAGGCTGAGGGAACCTTT<br /> |rRNA<br /> |-<br /> |GACCCGTCGGTCTCACAGTCAAGCTCCCTTGTGCACTTAC<br /> |rRNA<br /> |-<br /> |CTTCCACAAGCCACCGCCGGATCACTAGTCCCGACTTTCGTCCCTG<br /> |rRNA<br /> |-<br /> |GTACCTTTTATCCGTTGAGCGACGGCGCTTCCACAAGCCAC<br /> |rRNA<br /> |-<br /> |CAAACCATCCCGTCGATATGGACTCTTGGGGAAGATCAGCCTGTTATC<br /> |rRNA<br /> |-<br /> |CCAGGATGCGACGAGCCGACATCGAGGTGCCAAACCATCC<br /> |rRNA<br /> |-<br /> |GTCTCACACGTATACGGTTTGGCCTCATCCGGTTTCGCTC<br /> |rRNA<br /> |-<br /> |CTTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTCCAG<br /> |rRNA<br /> |-<br /> |GAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCGCTTTAATG<br /> |rRNA<br /> |-<br /> |CTTCTCAAGACTCCTACGCGCACAGCGGATAGGGACCGAAC<br /> |rRNA<br /> |-<br /> |CACACCAGAGGTTCGTCCGTCCCGGTCCTCTCGTACTAGG<br /> |rRNA<br /> |-<br /> |TTAGATGCTTTCAGCGGTTATCCCTCCCGAACGTAGCCAACCAGCCATGCCCTTGGCA<br /> |rRNA<br /> |-<br /> |CTCATCTCGAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATC<br /> |rRNA<br /> |-<br /> |CAGTCGTCTACTGGGAGCCTTAACCCCTCAAGGGGGTGGGAGTCCTCATCTCGAA<br /> |rRNA<br /> |-<br /> |CTTCCAGATCCGGCCTATCAACCCAGTCGTCTACTGGGAG<br /> |rRNA<br /> |-<br /> |CAGACCGTAAAAGAGAGATCCCACAACCCCGCACACGCAACCCCTGCCGGGTCTCA<br /> |rRNA<br /> |-<br /> |TACATCAACGGTTTCTGACTCGTCTCACGGCCGGCAGACCGTAAAAGAGAGAT<br /> |rRNA<br /> |-<br /> |CTTTCGCATGTCCTTCGCCTACATCAACGGTTTCTGACTC<br /> |rRNA<br /> |-<br /> |ACTACGGGGGTCTTACCCTCTACGCCGGACCTTTCGCATG<br /> |rRNA<br /> |-<br /> |CTACTTGGGTGTCTCTCAAACGAGCCGTTGACGTTTCGACTAC<br /> |rRNA<br /> |-<br /> |GCCAGATTCACACGGGATTTCTCGGGCCCCGTGCTACTTG<br /> |rRNA<br /> |-<br /> |TATCCGCTATCGGTCACCAGGGAATATTTAGGCTTAGCGGGTGGTCCCGCCAGATT<br /> |rRNA<br /> |-<br /> |GTACTTTTCACCATTCCCTCACGGTACTATCCGCTATCGGTCAC<br /> |rRNA<br /> |-<br /> |CTTGTAGGCACACGGTTTCAGGTACTATTTCACTCCGCTCCC<br /> |rRNA<br /> |-<br /> |GGGCCTATCGTGGCCTCCCACGTCCTTCATCGGTTCCTGG<br /> |rRNA<br /> |-<br /> |CACGCAGTCACGAGGATGGAGCAAGCTCCATCCCGACGCTCCCACGGCTTGTAGGCA<br /> |rRNA<br /> |-<br /> |CATTCTTCAAAAGGCACGCAGTCACGAGGATGGAGCAAGC<br /> |rRNA<br /> |-<br /> |TACGGCTTCCCCACTCGGGTTAACCTCGCAACACACCGCAAACTCGCAGGCTCAT<br /> |rRNA<br /> |-<br /> |TCGGACTCGCTTTCGCTACGGCTTCCCCACTCGGGTTAAC<br /> |rRNA<br /> |-<br /> |ATCACTCCGCTTCGGGTCTTGAGCGTGCTACTAAAAACGC<br /> |rRNA<br /> |-<br /> |CTCTTACAGCCGCTTCAACCTGCCCATGGCTAGATCACTC<br /> |rRNA<br /> |-<br /> |GTTTTCAACCCTGGTGGGTTCGGTCCTCCACGACCTCTTAC<br /> |rRNA<br /> |-<br /> |GTTTGATTGGCCTTTCACCCCTAACCACAGGTCATCCCCCAGGTTTTCAACC<br /> |rRNA<br /> |-<br /> |GGAGAACCAGCTATCACGGAGTTTGATTGGCCTTTCACCC<br /> |rRNA<br /> |-<br /> |TATCCAGTGCTCTACCTCCGGCAAGAAACACACGACGCTGCACCTAAATGCATTT<br /> |rRNA<br /> |-<br /> |GAGTTTGGCTAAGGTCAGTAACCCGGTAGGGCCCATCGCCTATCCAGTGCTCTA<br /> |rRNA<br /> |-<br /> |CTGTAAGGCTTAGCTTCCGGGTTCGAAATGTAACCGGGCGTTTCCCCTAC<br /> |rRNA<br /> |-<br /> |GTTCGGCGGCGTCCTACTCTCCCACAGGGTCCCCCCTGCAGTACCATCGGCGCTGTA<br /> |rRNA<br /> |-<br /> |GTATTAGACCCCGTTTCCAGGGCTTGTCCCAGAGTGAAGGGCAGATT<br /> |rRNA<br /> |-<br /> |GAGCTTTCGAACCTCGCAGATGCCTGCGAGGGTCAGTATCCGGTATTAGACCCCGTT<br /> |rRNA<br /> |-<br /> |CATCCTTCACCGCCGGAGCTTTCGAACCTCGCAGATGCCT<br /> |rRNA<br /> |-<br /> |CACCGAAGTGGTTCATCGTTCGACTTGCATGTGTTAAGCAC<br /> |rRNA<br /> |-<br /> |TTCGACTTGCATGTGTTAAGCACGCCGCCAGCGTTCGTCCTGAGCCAGGATCAAACTCT<br /> |rRNA<br /> |-<br /> |CAGATTGCCCACGTGTTACTCACCCGTTCGCCACTAATCC<br /> |rRNA<br /> |-<br /> |GCGGATTTACCTACCTGACGTCCTACACCCTTACCCCGGGACAACCACCGC<br /> |rRNA<br /> |-<br /> |CATCAGGTCTCAGACTCATGTCAGGCGGATTTACCTACCTGAC<br /> |rRNA<br /> |-<br /> |CTATTAGTACCGGTCACCTCCACACCTCACGGTGCTTCCAGATCCGGCCTATCAACC<br /> |rRNA<br /> |-<br /> |GGACAAGCCCTCGGCCTATTAGTACCGGTCACCTCCACAC<br /> |rRNA<br /> |-<br /> |CCGGGTTAACCTCGCAACACACCGCAAACTCGCAGGCTCATTCTTCAAAAGGCACGC<br /> |rRNA<br /> |-<br /> |GGTCTTGAGCGTGCTACTAAAACGCCCTATTCGGACTCGC<br /> |rRNA<br /> |-<br /> |TTCGGCGGCGTCCTACTCTCCCACAGGGTCCCCCCTGCAGTACCATCGGCGCTGTAA<br /> |rRNA<br /> |-<br /> |CCGAAGCCTCCGGCGGTCTGCTTTCTGTGGCACTGTCCCG<br /> |SCOs01<br /> |-<br /> |CTGGGCACTGGTGGTCTCTTACACCACCGTTTCACCCTTAC<br /> |SCOs01<br /> |-<br /> |TTCCTTCTTGACCTTGCTCCGGGTGGGGTTTACCTAGCTGCCCAGGTCGCCCTG<br /> |SCOs01<br /> |-<br /> |CTCGAACGTCCGCGCAGAAGCACCGGGGTGCTTCCTTCTTGACCTTGCTC<br /> |SCOs01<br /> |-<br /> |CTCCAGCGGTCTACCCGCGGACTCGGGCGGGCAGCCCTCGAACGTCCGCGCAGAAG<br /> |SCOs01<br /> |-<br /> |CATCCATCTAGGACCGGCGTTGCCACCGGCCTCCAGCGGTCTAC<br /> |SCOs01<br /> |-<br /> |CTGTGGAGCCCGGACGTTCCTCGGGAAGTCCCGTGAGGGACTC<br /> |SCOs01<br /> |-<br /> |GTTCTGTTCCGACGGGGCCTCGCGGTCCCGTCGGCGACGGCCATCCAT<br /> |SCOs01<br /> |-<br /> |CAGACGAGTCGGGCTGTACGCCGGGTTCTGTTCCGACGGG<br /> |SCOs01<br /> |-<br /> |GTCTGCTTTCTGTGGCACTGTCCCGCGGGTCACCCCGGGTGGCCGTTAGCCATCAC<br /> |SCOs01<br /> |-<br /> |CTTAGTAGGAGGCTCGCTTCGCTACCTGAGATCAGGCAGCGAGGGCGAAGG<br /> |SCOs02<br /> |-<br /> |GAACAGCCCCGGGCTGACACTCCCTTAGTAGGAGGCTCGCTTC<br /> |SCOs02<br /> |-<br /> |GATCAAGGTTTAGTCCCCTAGCTGATGCCAGGATCCGGGTC<br /> |SCOs02<br /> |-<br /> |CAGTCACTGTTTGGTTTCCCTCTTCACCCCGTGACCGGGATCAA<br /> |SCOs02<br /> |-<br /> |GAGATCACGCGGACAAGTCTCCGACGGGCCCAGTCACTGT<br /> |SCOs02<br /> |-<br /> |CTTCCCCTGCTTCGACAGCCGCTGTCGAAACCGATCATCC<br /> |SCOs02<br /> |-<br /> |AATCAGGGCTTCTCCGTGTGCAGTCCGCTTCGATTTTCTCGGCCCCGGAGATCACG<br /> |SCOs02<br /> |-<br /> |GAATCGAACCCGCGTCCAACGGTGCGGAATCAGGGCTTCTC<br /> |SCOs02<br /> |-<br /> |TTTTTCGGCCTGTGGTTTACGAGATCATGGCCGCTTCCTC<br /> |SCOs02<br /> |-<br /> |CTGCTGGGAGGAATCGCGCTTGGTGTTGGCGATTATTTTT<br /> |SCOs02<br /> |-<br /> |CACGCACCCGCCAGAGCCGACCTACCCTTGCTGCCTTCCG<br /> |SCOs03<br /> |}</div> RTEvans https://actinobase.org/index.php?title=RRNA_depletion_with_Streptomyces_coelicolor_A3(2)_specific_probes&diff=2188 RRNA depletion with Streptomyces coelicolor A3(2) specific probes 2023-11-01T16:39:03Z <p>RTEvans: /* Notes */</p> <hr /> <div>==Organisms==<br /> <br /> This protocol has been tested with &lt;em&gt;[[Streptomyces coelicolor]]&lt;/em&gt; A3(2) MT1110<br /> <br /> ==Description==<br /> <br /> rRNA depletion using commercial kits targeting generic bacterial sequences is not 100% efficient in the High GC organism S. coelicolor. <br /> <br /> Depletion using the NEBNext RNA Depletion Core Reagent Set with RNA Sample Purification Beads (https://www.neb.com/en-gb/products/e7870-nebnext-rna-depletion-core-reagent-set-with-rna-sample-purification-beads#Product%20Information) and custom DNA probes designed for &lt;em&gt;S. coelicolor&lt;/em&gt; is more effective than the QIAseq FastSelect 5S/16S/23S kit (Qiagen #335927); NEBNext rRNA Depletion Kit (Bacteria) (NEB #E7850); Illumina Ribo-zero rRNA Removal Kit (Bacteria) (Illumina #MRZMB12424, discontinued). The Illumina kit tested was subsequently replaced by the Illumina Ribo-Zero Plus rRNA Depletion Kit (Illumina #20040526). Additionally three abundant non-mRNA RNAs can be depleted (Table 1).<br /> <br /> '''Table 1.''' Abundant non-mRNA RNAs in &lt;em&gt;S. coelicolor&lt;/em&gt;.<br /> <br /> [[File: rRNA_depletion_table_1.png]]<br /> <br /> ==Materials==<br /> <br /> Single stranded DNA probes (Table 2) at 2µM concentration in IDTE pH 7.5 (10mM Tris-HCl / 0.1mM EDTA). Integrated DNA Technologies can produce these from their 25nmole DNA oligo pool product. Probes can be downloaded here [[Media: List_of_probes.xlsx]]<br /> <br /> NEBNext RNA Depletion Core Reagent Set with RNA Sample Purification Beads (https://www.neb.com/en-gb/products/e7870-nebnext-rna-depletion-core-reagent-set-with-rna-sample-purification-beads#Product%20Information).<br /> <br /> ==Protocols==<br /> <br /> As per manufacturer’s instructions.<br /> <br /> ==Notes==<br /> <br /> The following histograms illustrate effectiveness of rRNA depletion using various methods. Histograms show percentage of bases aligned to NCBI reference sequence NC_003888.3 using Hisat2 program classified as follows - Coding = RNA aligned to coding sequences; Transcribed not translated = RNA aligned to rRNA, tRNA and sRNA; Intergenic = RNA aligned to 5' or 3' UTRs or between annotated sequences.<br /> <br /> '''QIAseq FastSelect 5S/16S/23S kit'''<br /> <br /> [[File: PanelA.png]] <br /> <br /> '''NEBNext rRNA Depletion Kit (Bacteria)'''<br /> <br /> [[File: PanelB.png]]<br /> <br /> '''Illumina Ribo-zero rRNA Removal Kit (Bacteria)'''<br /> <br /> [[File: PanelC.png]] <br /> <br /> '''rRNA depletion with &lt;em&gt;S. coelicolor&lt;/em&gt; specific probes<br /> <br /> [[File: PanelD.png]]<br /> <br /> <br /> '''Table 2''' List of probes<br /> <br /> {| class=&quot;wikitable&quot;<br /> !Sequence<br /> !Target<br /> |-<br /> |CAAGTCCTTGCGCGACGCTCCCACGGCTTGTAGGCACACGGTTTCAGGTACTATTT<br /> |rRNA<br /> |-<br /> |CAGTCACGACGCAAGGACAAGTCCTTGCGCGACGCTCCCAC<br /> |rRNA<br /> |-<br /> |ACCTCGCAACACACCGCAAACTCGCAGGCTCATTCTTCAAAAGGCACGCAGTCACGACG<br /> |rRNA<br /> |-<br /> |CTACGGCTTCCCCACCCGGGTTAACCTCGCAACACACCGC<br /> |rRNA<br /> |-<br /> |TTAACCCAACATCTCACGACACGAGCTGACGACAGCCATGCACCACCTGTACACCGA<br /> |rRNA<br /> |-<br /> |CGCTCGTTGCGGGACTTAACCCAACATCTCACGACACGAG<br /> |rRNA<br /> |-<br /> |CAACACCCCGAAGGGCTTGCTGGCAACACGGGACAAGGGTTG<br /> |rRNA<br /> |-<br /> |CACCTTCCTCCGAGTTGACCCCGGCGGTCTCCCGTGAGTC<br /> |rRNA<br /> |-<br /> |GACATAAGGGGCATGATGACTTGACGTCGTCCCCACCTTC<br /> |rRNA<br /> |-<br /> |GTATCGCAGCTCATTGTACCGGCCATTGTAGCACGTGTGCAGCCCAAGACATAA<br /> |rRNA<br /> |-<br /> |TTTTGAGATTCGCTCCACCTTGCGGTATCGCAGCTCATTGTAC<br /> |rRNA<br /> |-<br /> |CAGACCCCAATCCGAACTGAGACCGGCTTTTTGAGATTCG<br /> |rRNA<br /> |-<br /> |AATGCTGATCTGCGATTACTAGCGACTCCGACTTCATGGGGTCGAGTTGCAGAC<br /> |rRNA<br /> |-<br /> |GAACGTATTCACCGCAGCAATGCTGATCTGCGATTACTAG<br /> |rRNA<br /> |-<br /> |TACCGACTTTCGTGACGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTC<br /> |rRNA<br /> |-<br /> |GCCACCGGCTTCGGGTGTTACCGACTTTCGTGACGTGACG<br /> |rRNA<br /> |-<br /> |TTCGTCCCAATCGCCAGTCCCACCTTCGACAGCTCCCTCCCACAAGGGGTTGGG<br /> |rRNA<br /> |-<br /> |CGCACCTTCCGGTACGGCTACCTTGTTACGACTTCGTCCCAATCGCCAGTCCCA<br /> |rRNA<br /> |-<br /> |TTCGACCCTCGCAGATGCCTGCGAGGGTCAGTATCCGGTATTAGACCCCGTTTCCAG<br /> |rRNA<br /> |-<br /> |GAAAGGAGGTGATCCAGCCGCACCTTCCGGTACGGCTACC<br /> |rRNA<br /> |-<br /> |TTCACCGCCGGAGCTTTCGACCCTCGCAGATGCCTGCGAG<br /> |rRNA<br /> |-<br /> |CATTACCTCACCAACAAGCTGATAGGCCGCGGGCTCATCCTTC<br /> |rRNA<br /> |-<br /> |CTCTCAGGCCGGCTACCCGTCGTCGCCTTGGTGAGCCATTAC<br /> |rRNA<br /> |-<br /> |GTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGGCCGGTCG<br /> |rRNA<br /> |-<br /> |TGCATCAGGCTTTCGCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCT<br /> |rRNA<br /> |-<br /> |TGCTGAAAGAGGTTTACAACCCGAAGGCCGTCATCCCTCACGCGGCGTCGCTGCATCA<br /> |rRNA<br /> |-<br /> |TTCTTCTGCAGGTACCGTCACTTTCGCTTCTTCCCTGCTGAAAGAGGTTTACAA<br /> |rRNA<br /> |-<br /> |CTACGTATTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTCTG<br /> |rRNA<br /> |-<br /> |CTCTTTACGCCCAATAATTCCGGACAACGCTTGCGCCCTACGTATTAC<br /> |rRNA<br /> |-<br /> |GGGCTTTCACAACCGACGTGACAAGCCGCCTACGAGCTCT<br /> |rRNA<br /> |-<br /> |CTACCGAACTCTAGCCTGCCCGTATCGACTGCAGACCCGGGGTTAAGCCCCG<br /> |rRNA<br /> |-<br /> |ATATCTGCGCATTTCACCGCTACACCAGGAATTCCGATCTCCCCTACCGAACTCTAG<br /> |rRNA<br /> |-<br /> |CGGTGTTCCTCCTGATATCTGCGCATTTCACCGCTACACC<br /> |rRNA<br /> |-<br /> |ACGCTTTCGCTCCTCAGCGTCAGTATCGGCCCAGAGATCCGCCTTCGCCACCGGTGT<br /> |rRNA<br /> |-<br /> |GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCG<br /> |rRNA<br /> |-<br /> |ACAACGTGGAATGTTGCCCACACCTAGTGCCCACCGTTTACGGCGTGGACTACCA<br /> |rRNA<br /> |-<br /> |CTTAATGCGTTAGCTGCGGCACGGACAACGTGGAATGTTG<br /> |rRNA<br /> |-<br /> |TCAATTCCTTTGAGTTTTAGCCTTGCGGCCGTACTCCCCAGGCGGGGCACTTAA<br /> |rRNA<br /> |-<br /> |ATTAAGCCACATGCTCCGCCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTTA<br /> |rRNA<br /> |-<br /> |TCAAGCCTTGGTAAGGTTCTTCGCGTTGCGTCGAATTAAGCCACATGCTCCGCCGCTT<br /> |rRNA<br /> |-<br /> |CATCTCTGATGCTTTCCGGTGTATGTCAAGCCTTGGTAAGGTTCTTC<br /> |rRNA<br /> |-<br /> |ACAGCCATGCACCACCTGTACACCGACCACAAGGGGGGCACCATCTCTGATGCTTTCC<br /> |rRNA<br /> |-<br /> |TGTAACCGGGCGTTTCCCCTACGCTATGACCACCGAAACC<br /> |rRNA<br /> |-<br /> |CAGTACCATCGGCGCTGTAAGGCTTAGCTTCCGGGTTCGAAATGTAAC<br /> |rRNA<br /> |-<br /> |GTTCGGCGGCGTCCTACTCTCCCACAGGGTCCCCCCTGCA<br /> |rRNA<br /> |-<br /> |CACTCCCCTCAACTCCGAAGAGATCAGGGCGGCTTCACGG<br /> |rRNA<br /> |-<br /> |CCATCACTCACCTACTAACCGCTTGGTTCGGCGGCTCCAC<br /> |rRNA<br /> |-<br /> |GTCCTTCATCGGTTCCTGGTGCCAAGGCATCCACCGTGCGCCCTTAAAAACTT<br /> |rRNA<br /> |-<br /> |CTTGTAGGCACACGGTTTCAGGTACTATTTCACTCCGCTCCCG<br /> |rRNA<br /> |-<br /> |CACGCAGTCACGAGACACCAAGCAAGCTTGATGTCCGACGCTCCCACGGCTTGTAGGCAC<br /> |rRNA<br /> |-<br /> |TCTTCAAAAGGCACGCAGTCACGAGACACCAAGCAAGCTT<br /> |rRNA<br /> |-<br /> |CACCCGGGTTAACCTCGCAACACACTGCAAACTCGCAGGC<br /> |rRNA<br /> |-<br /> |GCGTGCTACTAAAACGCCCTATTCGGACTCGCTTTCGCTACGGCTTCC<br /> |rRNA<br /> |-<br /> |GATCACTCCGCTTCGGGTCTTGAGCGTGCTACTAAAACGC<br /> |rRNA<br /> |-<br /> |TCGCAGATGCCTGCAAGGGTCAGTATCCGGTATTAGACCCCGTTT<br /> |rRNA<br /> |-<br /> |TCATCCTTCACCGCCGCAGCTTTCGAACCTCGCAGATGCC<br /> |rRNA<br /> |-<br /> |ATGTAACCGGGCGTTTCCCCTACGCTATGACCACCGAAAC<br /> |rRNA<br /> |-<br /> |CGTCCTACTCTCCCACAGGGTCCCCCCTGCAGTACCATCG<br /> |rRNA<br /> |-<br /> |CATCGCCTATCCAGTGCTCTACCTCCGGCAAGAAACACAC<br /> |rRNA<br /> |-<br /> |CATTCGGAGTTTGGCTAAGGTCAGTAACCCGGTAGGGCCCATCG<br /> |rRNA<br /> |-<br /> |CAGTCTCACTGCCGCGCTCTCACTTACCGGCATTCGGAGTTTG<br /> |rRNA<br /> |-<br /> |ATGCTCTGGGCTGTTTCCCTCTCGACCATGGAGCTTATCCCCCACAGTCTCACTG<br /> |rRNA<br /> |-<br /> |CTTAGGGGCCTTAGTCGATGCTCTGGGCTGTTTCCCTCTCG<br /> |rRNA<br /> |-<br /> |GTTGTCTGTGCGACTCCACATCCTTTCCCACTTAGCGTACGCTTAG<br /> |rRNA<br /> |-<br /> |TATTACGCACTCTTTCAAGGGTGGCTGCTTCTAAGCCAACCTCCTGGTTGTCTGT<br /> |rRNA<br /> |-<br /> |GATCAAAGCCTGGTTGACGACTCCCCGGGGCCTATCGTGG<br /> |rRNA<br /> |-<br /> |CGGAATCACTAGACCAGTGAGCTATTACGCACTCTTTCAAGG<br /> |rRNA<br /> |-<br /> |TATTGCTGCAATGACACGACTTCGGCGGTACGCTTGAGCCCCGCTACATTGTCG<br /> |rRNA<br /> |-<br /> |CATCACAGCCGGCGTTGGCCGTATTGCTGCAATGACACGACTTC<br /> |rRNA<br /> |-<br /> |TCCACAACTCGCTTCCGCGGCTGCTTCACCCGGCACACGACGCTCCCCTACCCATCACA<br /> |rRNA<br /> |-<br /> |TACTCATGCCTGCATTCTCACTCGTGAACCGTCCACAACTCGCTTCCGCGGCTGCTT<br /> |rRNA<br /> |-<br /> |CAAACGTTTCTCACGTTTGTATCGCTACTCATGCCTGCATTCTCACTC<br /> |rRNA<br /> |-<br /> |CAGGAACCCTTAGTCAATCGGCGCAAACGTTTCTCACGTTT<br /> |rRNA<br /> |-<br /> |CTTAGGTCCCGACTTACCCTGGGCAGATCAGCTTGACCCAG<br /> |rRNA<br /> |-<br /> |GAATATCAACCGGTTATCCATCGACTACGCCTGTCGGCCTCGCCTTAGGTC<br /> |rRNA<br /> |-<br /> |TTAGCATCACGATGCTCGATGTTTGACGCTTCACAGCGGGTACCGGAATATCAA<br /> |rRNA<br /> |-<br /> |GAAGAGATCAGGGCGGCTTCACGGCCTTAGCATCACGATG<br /> |rRNA<br /> |-<br /> |CATCACTCACCTACTAACCGCTTGGTTCGGCGGCTCCACC<br /> |rRNA<br /> |-<br /> |TGTGTTCAGCAGCGGGTGACAGCCCATGACGACTGCCGGGTTTCCCCATTCGGACACCC<br /> |rRNA<br /> |-<br /> |GACAACCACCGCCCGGGATGGACTACCTTCCTGCGTCACCCCAT<br /> |rRNA<br /> |-<br /> |CAGATGCTCGCGTCCACTGTGCAGTTCTCAAACAACGACC<br /> |rRNA<br /> |-<br /> |TGCGGATTTACCTACACACCGGCCTACACCCTTACCCCGGGACAACCACCG<br /> |rRNA<br /> |-<br /> |CATCAGGTCTCAGCCACAAGGTGTGCGGATTTACCTACAC<br /> |rRNA<br /> |-<br /> |TTTTCTCGACAGCATAGGATCATCCACTTCACCACAATCGGCTCGGCATCAGGTCT<br /> |rRNA<br /> |-<br /> |CCATGAAACTCGCTAGAGGCTTTTCTCGACAGCATAGGATCATC<br /> |rRNA<br /> |-<br /> |TATTCTCTACCTGACCACCTGAGTCGGTTTAGGGTACGGGCCGCCATGAAACT<br /> |rRNA<br /> |-<br /> |GAGTTCCTTAACCATAGTTCACCCGAACGCCTCGGTATTCTCTACCTGAC<br /> |rRNA<br /> |-<br /> |CTTCTCCCGAAGTTACGGGGGCATTTTGCCGAGTTCCTTAACCA<br /> |rRNA<br /> |-<br /> |CACCCCCAGCTCAGAGTGCAAAACTCGTCACCAGGTGTGG<br /> |rRNA<br /> |-<br /> |GTGTTTTTAGTAAACAGTCGCTTCTCGCTGGTCTCTGCGG<br /> |rRNA<br /> |-<br /> |GTTCCCTCCACACTGCCTATGTGTTCAGCAGCGGGTGACAG<br /> |rRNA<br /> |-<br /> |GTATACATCGCCTTACGGCTTCGCACGGACCTGTGTTTTTA<br /> |rRNA<br /> |-<br /> |CTTAACGTTCCAGCACCGGGCAGGCGTCAGTCCGTATACATC<br /> |rRNA<br /> |-<br /> |TAAGTTCTCAGCTTCGCCACACCGAAATGTGACTAACCGGTCCCCTTAACGTT<br /> |rRNA<br /> |-<br /> |TTAGGATGGTTATAGTTACCACCGCCGTTTACTGGCGCTTAAGTTCTCAG<br /> |rRNA<br /> |-<br /> |GTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGATGGTTATAGT<br /> |rRNA<br /> |-<br /> |TATGGTTGAGACAGTCGAGAAGTCGTTACGCCATTCGTGCAGGTCGGAACTTACC<br /> |rRNA<br /> |-<br /> |GAAACGAGCATCTTTACTCGTAGTGCAATTTCACCGGGCCTATGGTTGAG<br /> |rRNA<br /> |-<br /> |GGTCTTTCCGTCCTGCTGCGCGAAACGAGCATCTTTACTC<br /> |rRNA<br /> |-<br /> |GAACCGAACACCAATATCAAACTGTAGTAAAGGTCCCGGGGTCTTTC<br /> |rRNA<br /> |-<br /> |CTTCAAAGTCTCCCAGCTATCCTACACAAGCCGAACCGAACACCAATA<br /> |rRNA<br /> |-<br /> |AGACTGGTATTTCAACGACGACTCCACCCACACTGGCGTGCGAGCTTCAAAGTCT<br /> |rRNA<br /> |-<br /> |GTTAGACATCCAGCACGACCAGACTGGTATTTCAACGACGACTC<br /> |rRNA<br /> |-<br /> |TTAAACTACCCATCAGACACTGTCCCTGATCCGGATCACGGACCCAGGTTAGACAT<br /> |rRNA<br /> |-<br /> |GAATCACGGTTGTTTTCTCTTCCTGCGGGTACTGAGATGTTTCACTTC<br /> |rRNA<br /> |-<br /> |GTTACTCTTTAGGAGGCAACCGCCCCAGTTAAACTACCCATCAGACACTG<br /> |rRNA<br /> |-<br /> |ACTCAACACCTGATTGCCAACCAGGCTGAGGGAACCTTTGGGCGCCTCCGTTACT<br /> |rRNA<br /> |-<br /> |GTCTCACAGTCAAGCTCCCTTGTGCACTTACACTCAACACCTGATTG<br /> |rRNA<br /> |-<br /> |GACTTTCGTCCCTGCTCGACCCGTCGGTCTCACAGTCAAG<br /> |rRNA<br /> |-<br /> |TACCTTTTATCCGTTGAGCGACGGCGCTTCCACAAGCCACCGCCGGATCACTAGTC<br /> |rRNA<br /> |-<br /> |GTCGATATGGACTCTTGGGGAAGATCAGCCTGTTATCCCCGGGGTACCTTTTAT<br /> |rRNA<br /> |-<br /> |GACATCGAGGTGCCAAACCATCCCGTCGATATGGACTCTTG<br /> |rRNA<br /> |-<br /> |TTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTCCAGCCCCAGGATGCGACGA<br /> |rRNA<br /> |-<br /> |TCACGACGTTCTAAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACC<br /> |rRNA<br /> |-<br /> |GATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTC<br /> |rRNA<br /> |-<br /> |GTCCTCTCGTACTAGGGACAGCCCTTCTCAAGACTCCTAC<br /> |rRNA<br /> |-<br /> |TCTCACACGTATACGGTTTGGCCTCATCCGGTTTCGCTCGCCACTACTCCCGGAAT<br /> |rRNA<br /> |-<br /> |CATGCCCTTGGCAGGACAACTGGCACACCAGAGGTTCGTC<br /> |rRNA<br /> |-<br /> |CTTAGATGCTTTCAGCGGTTATCCCTCCCGAACGTAGCCAAC<br /> |rRNA<br /> |-<br /> |GTGGGAGTCCTCATCTCGAAGCAGGCTTCCCGCTTAGATG<br /> |rRNA<br /> |-<br /> |CTATCAACCCAGTCGTCTACTGGGAGCCTTAACCCCTCAAGG<br /> |rRNA<br /> |-<br /> |GCCTATTAGTACCGGTCACCTCCACACCTCACGGTGCTTCCAGATCCGGCCTATCAAC<br /> |rRNA<br /> |-<br /> |AGGACAAGCCCTCGGCCTATTAGTACCGGTCACCTCCACA<br /> |rRNA<br /> |-<br /> |CACACGCAACCCCTGCCGGGTCTCACACGTATACGGTTTG<br /> |rRNA<br /> |-<br /> |TTTCTGACTCGTCTCACGGCCGGCAGACCGTAAAAGAGAGATCCCACAACCCCGCACA<br /> |rRNA<br /> |-<br /> |TTTCGCATGTCCTTCGCCTACATCAACGGTTTCTGACTCGTCTCACGGCCGGCAGA<br /> |rRNA<br /> |-<br /> |CTTACCCTCTACGCCGGACCTTTCGCATGTCCTTCGCCTACATC<br /> |rRNA<br /> |-<br /> |GTGTCTCTCAAACGAGCCGTTGACGTTTCGACTACGGGGGTCTTACCCTCTA<br /> |rRNA<br /> |-<br /> |ATCCACCGTGCGCCCTTAAAAACTTGGCCACAGATGCTCG<br /> |rRNA<br /> |-<br /> |GATTTCTCGGGCCCCGTGCTACTTGGGTGTCTCTCAAACGAG<br /> |rRNA<br /> |-<br /> |GAATATTTAGGCTTAGCGGGTGGTCCCGCCAGATTCACACGGGATTTCTC<br /> |rRNA<br /> |-<br /> |CTCACGGTACTATCCGCTATCGGTCACCAGGGAATATTTAGGCTTAG<br /> |rRNA<br /> |-<br /> |GTTTCAGGTACTATTTCACTCCGCTCCCGCGGTACTTTTCACCATTC<br /> |rRNA<br /> |-<br /> |GATGGAGCAAGCTCCATCCCGACGCTCCCACGGCTTGTAGGCACACGGTTTCAGGTACT<br /> |rRNA<br /> |-<br /> |CACGCAGTCACGAGGATGGAGCAAGCTCCATCCCGACGCTC<br /> |rRNA<br /> |-<br /> |CTCGGGTTAACCTCGCAACACACCGCAAACTCGCAGGCTCATTCTTCAAAAGGCACGC<br /> |rRNA<br /> |-<br /> |CTACGGCTTCCCCACTCGGGTTAACCTCGCAACACACCGC<br /> |rRNA<br /> |-<br /> |GTCTTGAGCGTGCTACTAAAAACGCCCTATTCGGACTCGC<br /> |rRNA<br /> |-<br /> |CTCCCACGTCCTTCATCGGTTCCTGGTGCCAAGGCATCCAC<br /> |rRNA<br /> |-<br /> |CTTCAACCTGCCCATGGCTAGATCACTCCGCTTCGGGTCTTGAG<br /> |rRNA<br /> |-<br /> |GGTTCGGTCCTCCACGACCTCTTACAGCCGCTTCAACCTG<br /> |rRNA<br /> |-<br /> |CTAACCACAGGTCATCCCCCAGGTTTTCAACCCTGGTGGGTTCGGTCC<br /> |rRNA<br /> |-<br /> |AGAACCAGCTATCACGGAGTTTGATTGGCCTTTCACCCCTAACCACAGGTCATC<br /> |rRNA<br /> |-<br /> |CAAGAAACACACGACGCTGCACCTAAATGCATTTCGGGGAGAACCAGCTATCA<br /> |rRNA<br /> |-<br /> |TTTTAGCCTTGCGGCCGTACTCCCCAGGCGGGGCACTTAATGCGTTAGCTGCGGCAC<br /> |rRNA<br /> |-<br /> |CCGTCAATTCCTTTGAGTTTTAGCCTTGCGGCCGTACTCC<br /> |rRNA<br /> |-<br /> |AATAATCCGACCGTTCACAGCGTCCTCGCTGTTGTGTTACTTCAAAGGAA<br /> |rRNA<br /> |-<br /> |TTCTTCGCGTTGCGTCGAATTAAGCCACATGCTCCGCCGCTTGTGCGGGCCCCCGTCAA<br /> |rRNA<br /> |-<br /> |GTGTATGTCAAGCCTTGGTAAGGTTCTTCGCGTTGCGTCGAATTAAG<br /> |rRNA<br /> |-<br /> |GGGGGCACCATCTCTGATGCTTTCCGGTGTATGTCAAGCC<br /> |rRNA<br /> |-<br /> |TCACGACACGAGCTGACGACAGCCATGCACCACCTGTACACCGACCACAAGGGGGGCACC<br /> |rRNA<br /> |-<br /> |CTTAACCCAACATCTCACGACACGAGCTGACGACAGCCATG<br /> |rRNA<br /> |-<br /> |CAACACGGGACAAGGGTTGCGCTCGTTGCGGGACTTAACC<br /> |rRNA<br /> |-<br /> |GTCTCCCGTGAGTCCCCAACACCCCGAAGGGCTTGCTGGCAAC<br /> |rRNA<br /> |-<br /> |ACATAAGGGGCATGATGACTTGACGTCGTCCCCACCTTCCTCCGAGTTGACCCCG<br /> |rRNA<br /> |-<br /> |ATTGTACCGGCCATTGTAGCACGTGTGCAGCCCAAGACATAAGGGGCATGATGACTTGA<br /> |rRNA<br /> |-<br /> |TTTTGAGATTCGCTCCACCTTGCGGTATCGCAGCTCATTGTACCGGCCATTGTAGCACGT<br /> |rRNA<br /> |-<br /> |CAATCCGAACTGAGACCGGCTTTTTGAGATTCGCTCCACCTTGCGGTAT<br /> |rRNA<br /> |-<br /> |ATTACTAGCGACTCCGACTTCATGGGGTCGAGTTGCAGACCCCAATCCGAACTGAGA<br /> |rRNA<br /> |-<br /> |TATTCACCGCAGCAATGCTGATCTGCGATTACTAGCGACTCCGACTTCATG<br /> |rRNA<br /> |-<br /> |GTGTACAAGGCCCGGGAACGTATTCACCGCAGCAATGCTG<br /> |rRNA<br /> |-<br /> |GTAATCGGGTGACACCACGAGAGCGGAACAGTCGGAGGAATAA<br /> |rRNA<br /> |-<br /> |GTGTTACCGACTTTCGTGACGTGACGGGCGGTGTGTACAAG<br /> |rRNA<br /> |-<br /> |CACCTTCGACAGCTCCCTCCCACAAGGGGTTGGGCCACCGGCTTCGGGTGTTA<br /> |rRNA<br /> |-<br /> |CTACCTTGTTACGACTTCGTCCCAATCGCCAGTCCCACCTTCGACAG<br /> |rRNA<br /> |-<br /> |TCCTTAGAAAGGAGGTGATCCAGCCGCACCTTCCGGTACGGCTACCTTGTTACGA<br /> |rRNA<br /> |-<br /> |TGCAGTCGGCTATGTGCTCCTTAGAAAGGAGGTGATCCAG<br /> |rRNA<br /> |-<br /> |GATCAAACTCTCCGTGAATGTATACCCGTAATCGGGTGAC<br /> |rRNA<br /> |-<br /> |AAGTGGTTCATCGTTCGACTTGCATGTGTTAAGCACGCCGCCAGCGTTCGTCCTGA<br /> |rRNA<br /> |-<br /> |TATCAACATATCTGGCGTTGACTTTTGGCACGCTGTTGAGTTCTCAAGGAA<br /> |rRNA<br /> |-<br /> |TGTTACTCACCCGTTCGCCACTAATCCCCACCGAAGTGGTTCATCGTTCGACTT<br /> |rRNA<br /> |-<br /> |CTTGTCCCAGAGTGAAGGGCAGATTGCCCACGTGTTACTC<br /> |rRNA<br /> |-<br /> |GCAGATGCCTGCGAGGGTCAGTATCCGGTATTAGACCCCGTTTCCAGGGCTTGTCCCA<br /> |rRNA<br /> |-<br /> |ATCCTTCACCGCCGGAGCTTTCGAACCTCGCAGATGCCTGCGAGGGTCAGTATCC<br /> |rRNA<br /> |-<br /> |GATAGGCCGCGGGCTCATCCTTCACCGCCGGAGCTTTCGA<br /> |rRNA<br /> |-<br /> |GTCGCCTTGGTGAGCCATTACCTCACCAACAAGCTGATAG<br /> |rRNA<br /> |-<br /> |TAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGGCCGGTCGCCCTCTCAGGCCGGCTA<br /> |rRNA<br /> |-<br /> |ATTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTC<br /> |rRNA<br /> |-<br /> |CGTCGCTGCATCAGGCTTTCGCCCATTGTGCAATATTCCC<br /> |rRNA<br /> |-<br /> |CTTCTTCCCTGCTGAAAGAGGTTTACAACCCGAAGGCCGTCATC<br /> |rRNA<br /> |-<br /> |TTCAAAGGAACCTCAACCCGAACGGAGATCCGATCAGGTCGGGGTATCAACATAT<br /> |rRNA<br /> |-<br /> |GTAGTTAGCCGGCGCTTCTTCTGCAGGTACCGTCACTTTC<br /> |rRNA<br /> |-<br /> |ATAATTCCGGACAACGCTTGCGCCCTACGTATTACCGCGGCTGCTGGCACGTAGTTA<br /> |rRNA<br /> |-<br /> |CTTTCACAACCGACGTGACAAGCCGCCTACGAGCTCTTTACGCCCAATAATT<br /> |rRNA<br /> |-<br /> |TATCGACTGCAGACCCGGGGTTAAGCCCCGGGCTTTCACAACCGACGTGACAAG<br /> |rRNA<br /> |-<br /> |GATCTCCCCTACCGAACTCTAGCCTGCCCGTATCGACTGCAGAC<br /> |rRNA<br /> |-<br /> |CTGATATCTGCGCATTTCACCGCTACACCAGGAATTCCGATCTC<br /> |rRNA<br /> |-<br /> |CAGAGATCCGCCTTCGCCACCGGTGTTCCTCCTGATATCTG<br /> |rRNA<br /> |-<br /> |CACGCTTTCGCTCCTCAGCGTCAGTATCGGCCCAGAGATC<br /> |rRNA<br /> |-<br /> |CACCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTTC<br /> |rRNA<br /> |-<br /> |ACTTAATGCGTTAGCTGCGGCACGGACAACGTGGAATGTTGCCCACACCTAGTGCCCA<br /> |rRNA<br /> |-<br /> |GTTCGAAATGTAACCGGGCGTTTCCCCTACGCTATGACCACCGAAA<br /> |rRNA<br /> |-<br /> |CTTAGGATGGTTATAGTTACCACCGCCGTTTACTGGCGCTTAAGTTCTCA<br /> |rRNA<br /> |-<br /> |CCAAGCAAGCTTGATGTCCGACGCTCCCACGGCTTGTAGGCACACGGTTTCAGGTACTA<br /> |rRNA<br /> |-<br /> |CAGTCACGAGACACCAAGCAAGCTTGATGTCCGACGCTCC<br /> |rRNA<br /> |-<br /> |CTTGAGCGTGCTACTAAAAGCGCCCTATTCGGACTCGCTT<br /> |rRNA<br /> |-<br /> |CTACCTTGTTACGACTTCGTCCCAATCGCCAGTCCCACCTTCGACAGCTCCCT<br /> |rRNA<br /> |-<br /> |AAGGAGGTGATCCAGCCGCACCTTCCGGTACGGCTACCTTGTTACGACTTCGTC<br /> |rRNA<br /> |-<br /> |CCAGGATCAAACTCTCCGTGAATGTATACCCGTAATCGGG<br /> |rRNA<br /> |-<br /> |CTACCTTGTTACGACTTCGTCCCAATCGCCAGTCCCACCTTCG<br /> |rRNA<br /> |-<br /> |CTCTCACTTACCGGCATTCGGAGTTTGGCTAAGGTCAGTAAC<br /> |rRNA<br /> |-<br /> |CATTCGGACACCCCCGGATCAAAGCCTGGTTGACGACTCC<br /> |rRNA<br /> |-<br /> |GCTTATCCCCCACAGTCTCACTGCCGCGCTCTCACTTACC<br /> |rRNA<br /> |-<br /> |CTTAGTCGATGCTCTGGGCTGTTTCCCTCTCGACCATGGAGCTTATC<br /> |rRNA<br /> |-<br /> |CACATCCTTTCCCACTTAGCGTACGCTTAGGGGCCTTAGTCGATGCTCTG<br /> |rRNA<br /> |-<br /> |CTTCTAAGCCAACCTCCTGGTTGTCTGTGCGACTCCACATCCTTTC<br /> |rRNA<br /> |-<br /> |AATCACTAGACCAGTGAGCTATTACGCACTCTTTCAAGGGTGGCTGCTTCTAAGC<br /> |rRNA<br /> |-<br /> |CTACATTGTCGGCGCGGAATCACTAGACCAGTGAGCTATTA<br /> |rRNA<br /> |-<br /> |CGTATTGCTGCAATGACACGACTTCGGCGGTACGCTTGAG<br /> |rRNA<br /> |-<br /> |GCTCCCCTACCCATCACAGCCGGCGTTGGCCGTATTGCTG<br /> |rRNA<br /> |-<br /> |TTCTCACTCGTGAACCGTCCACAACTCGCTTCCGCGGCTGCTTCACCCGGCACACGACG<br /> |rRNA<br /> |-<br /> |GTTTGTATCGCTACTCATGCCTGCATTCTCACTCGTGAACCGTCCACAAC<br /> |rRNA<br /> |-<br /> |CTTAGTCAATCGGCGCAAACGTTTCTCACGTTTGTATCGCTACTCATG<br /> |rRNA<br /> |-<br /> |GACTTACCCTGGGCAGATCAGCTTGACCCAGGAACCCTTAGTCAATC<br /> |rRNA<br /> |-<br /> |TCCACACTGCCTATGTGTTCAGCAGCGGGTGACAGCCCATGACGACTGCCGGGTTT<br /> |rRNA<br /> |-<br /> |TTATCCATCGACTACGCCTGTCGGCCTCGCCTTAGGTCCCGACTTACCCTGGGCA<br /> |rRNA<br /> |-<br /> |GTACCGGAATATCAACCGGTTATCCATCGACTACGCCTGTC<br /> |rRNA<br /> |-<br /> |GGCCTTAGCATCACGATGCTCGATGTTTGACGCTTCACAG<br /> |rRNA<br /> |-<br /> |CTACTAACCGCTTGGTTCGGCGGCTCCACCACTCCCCTCAACTCCGAAGAGATCA<br /> |rRNA<br /> |-<br /> |GATGGACTACCTTCCTGCGTCACCCCATCACTCACCTACTAAC<br /> |rRNA<br /> |-<br /> |CCGGCCTACACCCTTACCCCGGGACAACCACCGCCCGGGATGGACTACC<br /> |rRNA<br /> |-<br /> |CAAGGCATCCACCGTGCGCCCTTAAAAACTTGGCCACAGA<br /> |rRNA<br /> |-<br /> |CAAGGTGTGCGGATTTACCTACACACCGGCCTACACCCTTAC<br /> |rRNA<br /> |-<br /> |CCACAATCGGCTCGGCATCAGGTCTCAGCCACAAGGTGTG<br /> |rRNA<br /> |-<br /> |CTAGAGGCTTTTCTCGACAGCATAGGATCATCCACTTCACCACAATC<br /> |rRNA<br /> |-<br /> |TTTAGGGTACGGGCCGCCATGAAACTCGCTAGAGGCTTTTCTC<br /> |rRNA<br /> |-<br /> |CTCGGTATTCTCTACCTGACCACCTGAGTCGGTTTAGGGTAC<br /> |rRNA<br /> |-<br /> |GGCATTTTGCCGAGTTCCTTAACCATAGTTCACCCGAACG<br /> |rRNA<br /> |-<br /> |CAGCTCAGAGTGCAAAACTCGTCACCAGGTGTGGCCCCCCTTCTCCCGAAGTTA<br /> |rRNA<br /> |-<br /> |TTGTTTTCTCTTCCTGCGGGTACTGAGATGTTTCACTTCCCCGCGTTCCCTCCACA<br /> |rRNA<br /> |-<br /> |TGTGTTTTTAGTAAACAGTCGCTTCTCGCTGGTCTCTGCGGCCACCCCCAGCTCAGA<br /> |rRNA<br /> |-<br /> |TATACATCGCCTTACGGCTTCGCACGGACCTGTGTTTTTAGTAAACAGTCGCTTCT<br /> |rRNA<br /> |-<br /> |GCAGGCGTCAGTCCGTATACATCGCCTTACGGCTTCGCAC<br /> |rRNA<br /> |-<br /> |GAAATGTGACTAACCGGTCCCCTTAACGTTCCAGCACCGGGCAGGC<br /> |rRNA<br /> |-<br /> |GTTTACTGGCGCTTAAGTTCTCAGCTTCGCCACACCGAAATGTGACTAAC<br /> |rRNA<br /> |-<br /> |GACAAGGAATTTCGCTACCTTAGGATGGTTATAGTTACCACCGCCGTTTA<br /> |rRNA<br /> |-<br /> |AAGTCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTA<br /> |rRNA<br /> |-<br /> |CTATGGTTGAGACAGTCGAGAAGTCGTTACGCCATTCGTG<br /> |rRNA<br /> |-<br /> |CTGCGCGAAACGAGCATCTTTACTCGTAGTGCAATTTCAC<br /> |rRNA<br /> |-<br /> |CAATATCAAACTGTAGTAAAGGTCCCGGGGTCTTTCCGTCCTGCTGCGCGAAA<br /> |rRNA<br /> |-<br /> |CAGCTATCCTACACAAGCCGAACCGAACACCAATATCAAACTGTAGTAAA<br /> |rRNA<br /> |-<br /> |CACACTGGCGTGCGAGCTTCAAAGTCTCCCAGCTATCCTACACAAG<br /> |rRNA<br /> |-<br /> |TTAGACATCCAGCACGACCAGACTGGTATTTCAACGACGACTCCACCCACACTGGCGTG<br /> |rRNA<br /> |-<br /> |GTTTCGCTCGCCACTACTCCCGGAATCACGGTTGTTTTCTCTTC<br /> |rRNA<br /> |-<br /> |GATCACGGACCCAGGTTAGACATCCAGCACGACCAGACTG<br /> |rRNA<br /> |-<br /> |CAGTTAAACTACCCATCAGACACTGTCCCTGATCCGGATCAC<br /> |rRNA<br /> |-<br /> |GAACCTTTGGGCGCCTCCGTTACTCTTTAGGAGGCAACCG<br /> |rRNA<br /> |-<br /> |CTTGTGCACTTACACTCAACACCTGATTGCCAACCAGGCTGAGGGAACCTTT<br /> |rRNA<br /> |-<br /> |GACCCGTCGGTCTCACAGTCAAGCTCCCTTGTGCACTTAC<br /> |rRNA<br /> |-<br /> |CTTCCACAAGCCACCGCCGGATCACTAGTCCCGACTTTCGTCCCTG<br /> |rRNA<br /> |-<br /> |GTACCTTTTATCCGTTGAGCGACGGCGCTTCCACAAGCCAC<br /> |rRNA<br /> |-<br /> |CAAACCATCCCGTCGATATGGACTCTTGGGGAAGATCAGCCTGTTATC<br /> |rRNA<br /> |-<br /> |CCAGGATGCGACGAGCCGACATCGAGGTGCCAAACCATCC<br /> |rRNA<br /> |-<br /> |GTCTCACACGTATACGGTTTGGCCTCATCCGGTTTCGCTC<br /> |rRNA<br /> |-<br /> |CTTTAATGGGCGAACAGCCCAACCCTTGGGACCGACTCCAG<br /> |rRNA<br /> |-<br /> |GAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCGCTTTAATG<br /> |rRNA<br /> |-<br /> |CTTCTCAAGACTCCTACGCGCACAGCGGATAGGGACCGAAC<br /> |rRNA<br /> |-<br /> |CACACCAGAGGTTCGTCCGTCCCGGTCCTCTCGTACTAGG<br /> |rRNA<br /> |-<br /> |TTAGATGCTTTCAGCGGTTATCCCTCCCGAACGTAGCCAACCAGCCATGCCCTTGGCA<br /> |rRNA<br /> |-<br /> |CTCATCTCGAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATC<br /> |rRNA<br /> |-<br /> |CAGTCGTCTACTGGGAGCCTTAACCCCTCAAGGGGGTGGGAGTCCTCATCTCGAA<br /> |rRNA<br /> |-<br /> |CTTCCAGATCCGGCCTATCAACCCAGTCGTCTACTGGGAG<br /> |rRNA<br /> |-<br /> |CAGACCGTAAAAGAGAGATCCCACAACCCCGCACACGCAACCCCTGCCGGGTCTCA<br /> |rRNA<br /> |-<br /> |TACATCAACGGTTTCTGACTCGTCTCACGGCCGGCAGACCGTAAAAGAGAGAT<br /> |rRNA<br /> |-<br /> |CTTTCGCATGTCCTTCGCCTACATCAACGGTTTCTGACTC<br /> |rRNA<br /> |-<br /> |ACTACGGGGGTCTTACCCTCTACGCCGGACCTTTCGCATG<br /> |rRNA<br /> |-<br /> |CTACTTGGGTGTCTCTCAAACGAGCCGTTGACGTTTCGACTAC<br /> |rRNA<br /> |-<br /> |GCCAGATTCACACGGGATTTCTCGGGCCCCGTGCTACTTG<br /> |rRNA<br /> |-<br /> |TATCCGCTATCGGTCACCAGGGAATATTTAGGCTTAGCGGGTGGTCCCGCCAGATT<br /> |rRNA<br /> |-<br /> |GTACTTTTCACCATTCCCTCACGGTACTATCCGCTATCGGTCAC<br /> |rRNA<br /> |-<br /> |CTTGTAGGCACACGGTTTCAGGTACTATTTCACTCCGCTCCC<br /> |rRNA<br /> |-<br /> |GGGCCTATCGTGGCCTCCCACGTCCTTCATCGGTTCCTGG<br /> |rRNA<br /> |-<br /> |CACGCAGTCACGAGGATGGAGCAAGCTCCATCCCGACGCTCCCACGGCTTGTAGGCA<br /> |rRNA<br /> |-<br /> |CATTCTTCAAAAGGCACGCAGTCACGAGGATGGAGCAAGC<br /> |rRNA<br /> |-<br /> |TACGGCTTCCCCACTCGGGTTAACCTCGCAACACACCGCAAACTCGCAGGCTCAT<br /> |rRNA<br /> |-<br /> |TCGGACTCGCTTTCGCTACGGCTTCCCCACTCGGGTTAAC<br /> |rRNA<br /> |-<br /> |ATCACTCCGCTTCGGGTCTTGAGCGTGCTACTAAAAACGC<br /> |rRNA<br /> |-<br /> |CTCTTACAGCCGCTTCAACCTGCCCATGGCTAGATCACTC<br /> |rRNA<br /> |-<br /> |GTTTTCAACCCTGGTGGGTTCGGTCCTCCACGACCTCTTAC<br /> |rRNA<br /> |-<br /> |GTTTGATTGGCCTTTCACCCCTAACCACAGGTCATCCCCCAGGTTTTCAACC<br /> |rRNA<br /> |-<br /> |GGAGAACCAGCTATCACGGAGTTTGATTGGCCTTTCACCC<br /> |rRNA<br /> |-<br /> |TATCCAGTGCTCTACCTCCGGCAAGAAACACACGACGCTGCACCTAAATGCATTT<br /> |rRNA<br /> |-<br /> |GAGTTTGGCTAAGGTCAGTAACCCGGTAGGGCCCATCGCCTATCCAGTGCTCTA<br /> |rRNA<br /> |-<br /> |CTGTAAGGCTTAGCTTCCGGGTTCGAAATGTAACCGGGCGTTTCCCCTAC<br /> |rRNA<br /> |-<br /> |GTTCGGCGGCGTCCTACTCTCCCACAGGGTCCCCCCTGCAGTACCATCGGCGCTGTA<br /> |rRNA<br /> |-<br /> |GTATTAGACCCCGTTTCCAGGGCTTGTCCCAGAGTGAAGGGCAGATT<br /> |rRNA<br /> |-<br /> |GAGCTTTCGAACCTCGCAGATGCCTGCGAGGGTCAGTATCCGGTATTAGACCCCGTT<br /> |rRNA<br /> |-<br /> |CATCCTTCACCGCCGGAGCTTTCGAACCTCGCAGATGCCT<br /> |rRNA<br /> |-<br /> |CACCGAAGTGGTTCATCGTTCGACTTGCATGTGTTAAGCAC<br /> |rRNA<br /> |-<br /> |TTCGACTTGCATGTGTTAAGCACGCCGCCAGCGTTCGTCCTGAGCCAGGATCAAACTCT<br /> |rRNA<br /> |-<br /> |CAGATTGCCCACGTGTTACTCACCCGTTCGCCACTAATCC<br /> |rRNA<br /> |-<br /> |GCGGATTTACCTACCTGACGTCCTACACCCTTACCCCGGGACAACCACCGC<br /> |rRNA<br /> |-<br /> |CATCAGGTCTCAGACTCATGTCAGGCGGATTTACCTACCTGAC<br /> |rRNA<br /> |-<br /> |CTATTAGTACCGGTCACCTCCACACCTCACGGTGCTTCCAGATCCGGCCTATCAACC<br /> |rRNA<br /> |-<br /> |GGACAAGCCCTCGGCCTATTAGTACCGGTCACCTCCACAC<br /> |rRNA<br /> |-<br /> |CCGGGTTAACCTCGCAACACACCGCAAACTCGCAGGCTCATTCTTCAAAAGGCACGC<br /> |rRNA<br /> |-<br /> |GGTCTTGAGCGTGCTACTAAAACGCCCTATTCGGACTCGC<br /> |rRNA<br /> |-<br /> |TTCGGCGGCGTCCTACTCTCCCACAGGGTCCCCCCTGCAGTACCATCGGCGCTGTAA<br /> |rRNA<br /> |-<br /> |CCGAAGCCTCCGGCGGTCTGCTTTCTGTGGCACTGTCCCG<br /> |SCOs01<br /> |-<br /> |CTGGGCACTGGTGGTCTCTTACACCACCGTTTCACCCTTAC<br /> |SCOs01<br /> |-<br /> |TTCCTTCTTGACCTTGCTCCGGGTGGGGTTTACCTAGCTGCCCAGGTCGCCCTG<br /> |SCOs01<br /> |-<br /> |CTCGAACGTCCGCGCAGAAGCACCGGGGTGCTTCCTTCTTGACCTTGCTC<br /> |SCOs01<br /> |-<br /> |CTCCAGCGGTCTACCCGCGGACTCGGGCGGGCAGCCCTCGAACGTCCGCGCAGAAG<br /> |SCOs01<br /> |-<br /> |CATCCATCTAGGACCGGCGTTGCCACCGGCCTCCAGCGGTCTAC<br /> |SCOs01<br /> |-<br /> |CTGTGGAGCCCGGACGTTCCTCGGGAAGTCCCGTGAGGGACTC<br /> |SCOs01<br /> |-<br /> |GTTCTGTTCCGACGGGGCCTCGCGGTCCCGTCGGCGACGGCCATCCAT<br /> |SCOs01<br /> |-<br /> |CAGACGAGTCGGGCTGTACGCCGGGTTCTGTTCCGACGGG<br /> |SCOs01<br /> |-<br /> |GTCTGCTTTCTGTGGCACTGTCCCGCGGGTCACCCCGGGTGGCCGTTAGCCATCAC<br /> |SCOs01<br /> |-<br /> |CTTAGTAGGAGGCTCGCTTCGCTACCTGAGATCAGGCAGCGAGGGCGAAGG<br /> |SCOs02<br /> |-<br /> |GAACAGCCCCGGGCTGACACTCCCTTAGTAGGAGGCTCGCTTC<br /> |SCOs02<br /> |-<br /> |GATCAAGGTTTAGTCCCCTAGCTGATGCCAGGATCCGGGTC<br /> |SCOs02<br /> |-<br /> |CAGTCACTGTTTGGTTTCCCTCTTCACCCCGTGACCGGGATCAA<br /> |SCOs02<br /> |-<br /> |GAGATCACGCGGACAAGTCTCCGACGGGCCCAGTCACTGT<br /> |SCOs02<br /> |-<br /> |CTTCCCCTGCTTCGACAGCCGCTGTCGAAACCGATCATCC<br /> |SCOs02<br /> |-<br /> |AATCAGGGCTTCTCCGTGTGCAGTCCGCTTCGATTTTCTCGGCCCCGGAGATCACG<br /> |SCOs02<br /> |-<br /> |GAATCGAACCCGCGTCCAACGGTGCGGAATCAGGGCTTCTC<br /> |SCOs02<br /> |-<br /> |TTTTTCGGCCTGTGGTTTACGAGATCATGGCCGCTTCCTC<br /> |SCOs02<br /> |-<br /> |CTGCTGGGAGGAATCGCGCTTGGTGTTGGCGATTATTTTT<br /> |SCOs02<br /> |-<br /> |CACGCACCCGCCAGAGCCGACCTACCCTTGCTGCCTTCCG<br /> |SCOs03<br /> |}</div> RTEvans https://actinobase.org/index.php?title=RRNA_depletion_with_Streptomyces_coelicolor_A3(2)_specific_probes&diff=2187 RRNA depletion with Streptomyces coelicolor A3(2) specific probes 2023-11-01T16:14:57Z <p>RTEvans: /* Materials */</p> <hr /> <div>==Organisms==<br /> <br /> This protocol has been tested with &lt;em&gt;[[Streptomyces coelicolor]]&lt;/em&gt; A3(2) MT1110<br /> <br /> ==Description==<br /> <br /> rRNA depletion using commercial kits targeting generic bacterial sequences is not 100% efficient in the High GC organism S. coelicolor. <br /> <br /> Depletion using the NEBNext RNA Depletion Core Reagent Set with RNA Sample Purification Beads (https://www.neb.com/en-gb/products/e7870-nebnext-rna-depletion-core-reagent-set-with-rna-sample-purification-beads#Product%20Information) and custom DNA probes designed for &lt;em&gt;S. coelicolor&lt;/em&gt; is more effective than the QIAseq FastSelect 5S/16S/23S kit (Qiagen #335927); NEBNext rRNA Depletion Kit (Bacteria) (NEB #E7850); Illumina Ribo-zero rRNA Removal Kit (Bacteria) (Illumina #MRZMB12424, discontinued). The Illumina kit tested was subsequently replaced by the Illumina Ribo-Zero Plus rRNA Depletion Kit (Illumina #20040526). Additionally three abundant non-mRNA RNAs can be depleted (Table 1).<br /> <br /> '''Table 1.''' Abundant non-mRNA RNAs in &lt;em&gt;S. coelicolor&lt;/em&gt;.<br /> <br /> [[File: rRNA_depletion_table_1.png]]<br /> <br /> ==Materials==<br /> <br /> Single stranded DNA probes (Table 2) at 2µM concentration in IDTE pH 7.5 (10mM Tris-HCl / 0.1mM EDTA). Integrated DNA Technologies can produce these from their 25nmole DNA oligo pool product. Probes can be downloaded here [[Media: List_of_probes.xlsx]]<br /> <br /> NEBNext RNA Depletion Core Reagent Set with RNA Sample Purification Beads (https://www.neb.com/en-gb/products/e7870-nebnext-rna-depletion-core-reagent-set-with-rna-sample-purification-beads#Product%20Information).<br /> <br /> ==Protocols==<br /> <br /> As per manufacturer’s instructions.<br /> <br /> ==Notes==<br /> <br /> The following histograms illustrate effectiveness of rRNA depletion using various methods. Histograms show percentage of bases aligned to NCBI reference sequence NC_003888.3 using Hisat2 program classified as follows - Coding = RNA aligned to coding sequences; Transcribed not translated = RNA aligned to rRNA, tRNA and sRNA; Intergenic = RNA aligned to 5' or 3' UTRs or between annotated sequences.<br /> <br /> '''QIAseq FastSelect 5S/16S/23S kit'''<br /> <br /> [[File: PanelA.png]] <br /> <br /> '''NEBNext rRNA Depletion Kit (Bacteria)'''<br /> <br /> [[File: PanelB.png]]<br /> <br /> '''Illumina Ribo-zero rRNA Removal Kit (Bacteria)'''<br /> <br /> [[File: PanelC.png]] <br /> <br /> '''rRNA depletion with &lt;em&gt;S. coelicolor&lt;/em&gt; specific probes<br /> <br /> [[File: PanelD.png]]</div> RTEvans https://actinobase.org/index.php?title=RRNA_depletion_with_Streptomyces_coelicolor_A3(2)_specific_probes&diff=2186 RRNA depletion with Streptomyces coelicolor A3(2) specific probes 2023-11-01T16:13:11Z <p>RTEvans: /* Materials */</p> <hr /> <div>==Organisms==<br /> <br /> This protocol has been tested with &lt;em&gt;[[Streptomyces coelicolor]]&lt;/em&gt; A3(2) MT1110<br /> <br /> ==Description==<br /> <br /> rRNA depletion using commercial kits targeting generic bacterial sequences is not 100% efficient in the High GC organism S. coelicolor. <br /> <br /> Depletion using the NEBNext RNA Depletion Core Reagent Set with RNA Sample Purification Beads (https://www.neb.com/en-gb/products/e7870-nebnext-rna-depletion-core-reagent-set-with-rna-sample-purification-beads#Product%20Information) and custom DNA probes designed for &lt;em&gt;S. coelicolor&lt;/em&gt; is more effective than the QIAseq FastSelect 5S/16S/23S kit (Qiagen #335927); NEBNext rRNA Depletion Kit (Bacteria) (NEB #E7850); Illumina Ribo-zero rRNA Removal Kit (Bacteria) (Illumina #MRZMB12424, discontinued). The Illumina kit tested was subsequently replaced by the Illumina Ribo-Zero Plus rRNA Depletion Kit (Illumina #20040526). Additionally three abundant non-mRNA RNAs can be depleted (Table 1).<br /> <br /> '''Table 1.''' Abundant non-mRNA RNAs in &lt;em&gt;S. coelicolor&lt;/em&gt;.<br /> <br /> [[File: rRNA_depletion_table_1.png]]<br /> <br /> ==Materials==<br /> <br /> Single stranded DNA probes (Table 2) at 2µM concentration in IDTE pH 7.5 (10mM Tris-HCl / 0.1mM EDTA). Integrated DNA Technologies can produce these from their 25nmole DNA oligo pool product. Probes can be downloaded here [[File: List_of_probes.xlsx]]<br /> <br /> NEBNext RNA Depletion Core Reagent Set with RNA Sample Purification Beads (https://www.neb.com/en-gb/products/e7870-nebnext-rna-depletion-core-reagent-set-with-rna-sample-purification-beads#Product%20Information).<br /> <br /> ==Protocols==<br /> <br /> As per manufacturer’s instructions.<br /> <br /> ==Notes==<br /> <br /> The following histograms illustrate effectiveness of rRNA depletion using various methods. Histograms show percentage of bases aligned to NCBI reference sequence NC_003888.3 using Hisat2 program classified as follows - Coding = RNA aligned to coding sequences; Transcribed not translated = RNA aligned to rRNA, tRNA and sRNA; Intergenic = RNA aligned to 5' or 3' UTRs or between annotated sequences.<br /> <br /> '''QIAseq FastSelect 5S/16S/23S kit'''<br /> <br /> [[File: PanelA.png]] <br /> <br /> '''NEBNext rRNA Depletion Kit (Bacteria)'''<br /> <br /> [[File: PanelB.png]]<br /> <br /> '''Illumina Ribo-zero rRNA Removal Kit (Bacteria)'''<br /> <br /> [[File: PanelC.png]] <br /> <br /> '''rRNA depletion with &lt;em&gt;S. coelicolor&lt;/em&gt; specific probes<br /> <br /> [[File: PanelD.png]]</div> RTEvans https://actinobase.org/index.php?title=RRNA_depletion_with_Streptomyces_coelicolor_A3(2)_specific_probes&diff=2185 RRNA depletion with Streptomyces coelicolor A3(2) specific probes 2023-11-01T16:12:25Z <p>RTEvans: /* Materials */</p> <hr /> <div>==Organisms==<br /> <br /> This protocol has been tested with &lt;em&gt;[[Streptomyces coelicolor]]&lt;/em&gt; A3(2) MT1110<br /> <br /> ==Description==<br /> <br /> rRNA depletion using commercial kits targeting generic bacterial sequences is not 100% efficient in the High GC organism S. coelicolor. <br /> <br /> Depletion using the NEBNext RNA Depletion Core Reagent Set with RNA Sample Purification Beads (https://www.neb.com/en-gb/products/e7870-nebnext-rna-depletion-core-reagent-set-with-rna-sample-purification-beads#Product%20Information) and custom DNA probes designed for &lt;em&gt;S. coelicolor&lt;/em&gt; is more effective than the QIAseq FastSelect 5S/16S/23S kit (Qiagen #335927); NEBNext rRNA Depletion Kit (Bacteria) (NEB #E7850); Illumina Ribo-zero rRNA Removal Kit (Bacteria) (Illumina #MRZMB12424, discontinued). The Illumina kit tested was subsequently replaced by the Illumina Ribo-Zero Plus rRNA Depletion Kit (Illumina #20040526). Additionally three abundant non-mRNA RNAs can be depleted (Table 1).<br /> <br /> '''Table 1.''' Abundant non-mRNA RNAs in &lt;em&gt;S. coelicolor&lt;/em&gt;.<br /> <br /> [[File: rRNA_depletion_table_1.png]]<br /> <br /> ==Materials==<br /> <br /> Single stranded DNA probes (Table 2) at 2µM concentration in IDTE pH 7.5 (10mM Tris-HCl / 0.1mM EDTA). Integrated DNA Technologies can produce these from their 25nmole DNA oligo pool product. Probes can be downloaded here [:File: List_of_probes.xlsx]<br /> <br /> NEBNext RNA Depletion Core Reagent Set with RNA Sample Purification Beads (https://www.neb.com/en-gb/products/e7870-nebnext-rna-depletion-core-reagent-set-with-rna-sample-purification-beads#Product%20Information).<br /> <br /> ==Protocols==<br /> <br /> As per manufacturer’s instructions.<br /> <br /> ==Notes==<br /> <br /> The following histograms illustrate effectiveness of rRNA depletion using various methods. Histograms show percentage of bases aligned to NCBI reference sequence NC_003888.3 using Hisat2 program classified as follows - Coding = RNA aligned to coding sequences; Transcribed not translated = RNA aligned to rRNA, tRNA and sRNA; Intergenic = RNA aligned to 5' or 3' UTRs or between annotated sequences.<br /> <br /> '''QIAseq FastSelect 5S/16S/23S kit'''<br /> <br /> [[File: PanelA.png]] <br /> <br /> '''NEBNext rRNA Depletion Kit (Bacteria)'''<br /> <br /> [[File: PanelB.png]]<br /> <br /> '''Illumina Ribo-zero rRNA Removal Kit (Bacteria)'''<br /> <br /> [[File: PanelC.png]] <br /> <br /> '''rRNA depletion with &lt;em&gt;S. coelicolor&lt;/em&gt; specific probes<br /> <br /> [[File: PanelD.png]]</div> RTEvans https://actinobase.org/index.php?title=RRNA_depletion_with_Streptomyces_coelicolor_A3(2)_specific_probes&diff=2184 RRNA depletion with Streptomyces coelicolor A3(2) specific probes 2023-11-01T16:11:31Z <p>RTEvans: /* Notes */</p> <hr /> <div>==Organisms==<br /> <br /> This protocol has been tested with &lt;em&gt;[[Streptomyces coelicolor]]&lt;/em&gt; A3(2) MT1110<br /> <br /> ==Description==<br /> <br /> rRNA depletion using commercial kits targeting generic bacterial sequences is not 100% efficient in the High GC organism S. coelicolor. <br /> <br /> Depletion using the NEBNext RNA Depletion Core Reagent Set with RNA Sample Purification Beads (https://www.neb.com/en-gb/products/e7870-nebnext-rna-depletion-core-reagent-set-with-rna-sample-purification-beads#Product%20Information) and custom DNA probes designed for &lt;em&gt;S. coelicolor&lt;/em&gt; is more effective than the QIAseq FastSelect 5S/16S/23S kit (Qiagen #335927); NEBNext rRNA Depletion Kit (Bacteria) (NEB #E7850); Illumina Ribo-zero rRNA Removal Kit (Bacteria) (Illumina #MRZMB12424, discontinued). The Illumina kit tested was subsequently replaced by the Illumina Ribo-Zero Plus rRNA Depletion Kit (Illumina #20040526). Additionally three abundant non-mRNA RNAs can be depleted (Table 1).<br /> <br /> '''Table 1.''' Abundant non-mRNA RNAs in &lt;em&gt;S. coelicolor&lt;/em&gt;.<br /> <br /> [[File: rRNA_depletion_table_1.png]]<br /> <br /> ==Materials==<br /> <br /> Single stranded DNA probes (Table 2) at 2µM concentration in IDTE pH 7.5 (10mM Tris-HCl / 0.1mM EDTA). Integrated DNA Technologies can produce these from their 25nmole DNA oligo pool product. Probes are listed in Table 2 in the Notes section below.<br /> <br /> NEBNext RNA Depletion Core Reagent Set with RNA Sample Purification Beads (https://www.neb.com/en-gb/products/e7870-nebnext-rna-depletion-core-reagent-set-with-rna-sample-purification-beads#Product%20Information).<br /> <br /> ==Protocols==<br /> <br /> As per manufacturer’s instructions.<br /> <br /> ==Notes==<br /> <br /> The following histograms illustrate effectiveness of rRNA depletion using various methods. Histograms show percentage of bases aligned to NCBI reference sequence NC_003888.3 using Hisat2 program classified as follows - Coding = RNA aligned to coding sequences; Transcribed not translated = RNA aligned to rRNA, tRNA and sRNA; Intergenic = RNA aligned to 5' or 3' UTRs or between annotated sequences.<br /> <br /> '''QIAseq FastSelect 5S/16S/23S kit'''<br /> <br /> [[File: PanelA.png]] <br /> <br /> '''NEBNext rRNA Depletion Kit (Bacteria)'''<br /> <br /> [[File: PanelB.png]]<br /> <br /> '''Illumina Ribo-zero rRNA Removal Kit (Bacteria)'''<br /> <br /> [[File: PanelC.png]] <br /> <br /> '''rRNA depletion with &lt;em&gt;S. coelicolor&lt;/em&gt; specific probes<br /> <br /> [[File: PanelD.png]]</div> RTEvans https://actinobase.org/index.php?title=RRNA_depletion_with_Streptomyces_coelicolor_A3(2)_specific_probes&diff=2183 RRNA depletion with Streptomyces coelicolor A3(2) specific probes 2023-11-01T16:08:58Z <p>RTEvans: /* Description */</p> <hr /> <div>==Organisms==<br /> <br /> This protocol has been tested with &lt;em&gt;[[Streptomyces coelicolor]]&lt;/em&gt; A3(2) MT1110<br /> <br /> ==Description==<br /> <br /> rRNA depletion using commercial kits targeting generic bacterial sequences is not 100% efficient in the High GC organism S. coelicolor. <br /> <br /> Depletion using the NEBNext RNA Depletion Core Reagent Set with RNA Sample Purification Beads (https://www.neb.com/en-gb/products/e7870-nebnext-rna-depletion-core-reagent-set-with-rna-sample-purification-beads#Product%20Information) and custom DNA probes designed for &lt;em&gt;S. coelicolor&lt;/em&gt; is more effective than the QIAseq FastSelect 5S/16S/23S kit (Qiagen #335927); NEBNext rRNA Depletion Kit (Bacteria) (NEB #E7850); Illumina Ribo-zero rRNA Removal Kit (Bacteria) (Illumina #MRZMB12424, discontinued). The Illumina kit tested was subsequently replaced by the Illumina Ribo-Zero Plus rRNA Depletion Kit (Illumina #20040526). Additionally three abundant non-mRNA RNAs can be depleted (Table 1).<br /> <br /> '''Table 1.''' Abundant non-mRNA RNAs in &lt;em&gt;S. coelicolor&lt;/em&gt;.<br /> <br /> [[File: rRNA_depletion_table_1.png]]<br /> <br /> ==Materials==<br /> <br /> Single stranded DNA probes (Table 2) at 2µM concentration in IDTE pH 7.5 (10mM Tris-HCl / 0.1mM EDTA). Integrated DNA Technologies can produce these from their 25nmole DNA oligo pool product. Probes are listed in Table 2 in the Notes section below.<br /> <br /> NEBNext RNA Depletion Core Reagent Set with RNA Sample Purification Beads (https://www.neb.com/en-gb/products/e7870-nebnext-rna-depletion-core-reagent-set-with-rna-sample-purification-beads#Product%20Information).<br /> <br /> ==Protocols==<br /> <br /> As per manufacturer’s instructions.<br /> <br /> ==Notes==<br /> <br /> The following histograms illustrate effectiveness of rRNA depletion using various methods. Histograms show percentage of bases aligned to NCBI reference sequence NC_003888.3 using Hisat2 program classified as follows - Coding = RNA aligned to coding sequences; Transcribed not translated = RNA aligned to rRNA, tRNA and sRNA; Intergenic = RNA aligned to 5' or 3' UTRs or between annotated sequences.<br /> <br /> '''QIAseq FastSelect 5S/16S/23S kit'''<br /> <br /> [[File: PanelA.png]] <br /> <br /> '''NEBNext rRNA Depletion Kit (Bacteria)'''<br /> <br /> [[File: PanelB.png]]<br /> <br /> '''Illumina Ribo-zero rRNA Removal Kit (Bacteria)'''<br /> <br /> [[File: PanelC.png]] <br /> <br /> '''rRNA depletion with &lt;em&gt;S. coelicolor&lt;/em&gt; specific probes<br /> <br /> [[File: PanelD.png]]<br /> <br /> '''Table 2''' - List of probes<br /> <br /> {| class=&quot;wikitable&quot;<br /> !Sequence<br /> !Target<br /> |-<br /> |CAAGTCCTTGCGCGACGCTCCCACGGCTTGTAGGCACACGGTTTCAGGTACTATTT<br /> |rRNA<br /> |-<br /> |CAGTCACGACGCAAGGACAAGTCCTTGCGCGACGCTCCCAC<br /> |rRNA<br /> |}</div> RTEvans https://actinobase.org/index.php?title=RRNA_depletion_with_Streptomyces_coelicolor_A3(2)_specific_probes&diff=2182 RRNA depletion with Streptomyces coelicolor A3(2) specific probes 2023-11-01T16:08:15Z <p>RTEvans: /* Notes */</p> <hr /> <div>==Organisms==<br /> <br /> This protocol has been tested with &lt;em&gt;[[Streptomyces coelicolor]]&lt;/em&gt; A3(2) MT1110<br /> <br /> ==Description==<br /> <br /> rRNA depletion using commercial kits targeting generic bacterial sequences is not 100% efficient in the High GC organism S. coelicolor. <br /> <br /> Depletion using the NEBNext RNA Depletion Core Reagent Set with RNA Sample Purification Beads (https://www.neb.com/en-gb/products/e7870-nebnext-rna-depletion-core-reagent-set-with-rna-sample-purification-beads#Product%20Information) and custom DNA probes designed for &lt;em&gt;S. coelicolor&lt;/em&gt; is more effective than the QIAseq FastSelect 5S/16S/23S kit (Qiagen #335927); NEBNext rRNA Depletion Kit (Bacteria) (NEB #E7850); Illumina Ribo-zero rRNA Removal Kit (Bacteria) (Illumina #MRZMB12424, discontinued). The Illumina kit tested was subsequently replaced by the Illumina Ribo-Zero Plus rRNA Depletion Kit (Illumina #20040526). Additionally three abundant non-mRNA RNAs can be depleted (Table 1).<br /> <br /> Table 1. Abundant non-mRNA RNAs in &lt;em&gt;S. coelicolor&lt;/em&gt;.<br /> <br /> [[File: rRNA_depletion_table_1.png]]<br /> <br /> ==Materials==<br /> <br /> Single stranded DNA probes (Table 2) at 2µM concentration in IDTE pH 7.5 (10mM Tris-HCl / 0.1mM EDTA). Integrated DNA Technologies can produce these from their 25nmole DNA oligo pool product. Probes are listed in Table 2 in the Notes section below.<br /> <br /> NEBNext RNA Depletion Core Reagent Set with RNA Sample Purification Beads (https://www.neb.com/en-gb/products/e7870-nebnext-rna-depletion-core-reagent-set-with-rna-sample-purification-beads#Product%20Information).<br /> <br /> ==Protocols==<br /> <br /> As per manufacturer’s instructions.<br /> <br /> ==Notes==<br /> <br /> The following histograms illustrate effectiveness of rRNA depletion using various methods. Histograms show percentage of bases aligned to NCBI reference sequence NC_003888.3 using Hisat2 program classified as follows - Coding = RNA aligned to coding sequences; Transcribed not translated = RNA aligned to rRNA, tRNA and sRNA; Intergenic = RNA aligned to 5' or 3' UTRs or between annotated sequences.<br /> <br /> '''QIAseq FastSelect 5S/16S/23S kit'''<br /> <br /> [[File: PanelA.png]] <br /> <br /> '''NEBNext rRNA Depletion Kit (Bacteria)'''<br /> <br /> [[File: PanelB.png]]<br /> <br /> '''Illumina Ribo-zero rRNA Removal Kit (Bacteria)'''<br /> <br /> [[File: PanelC.png]] <br /> <br /> '''rRNA depletion with &lt;em&gt;S. coelicolor&lt;/em&gt; specific probes<br /> <br /> [[File: PanelD.png]]<br /> <br /> '''Table 2''' - List of probes<br /> <br /> {| class=&quot;wikitable&quot;<br /> !Sequence<br /> !Target<br /> |-<br /> |CAAGTCCTTGCGCGACGCTCCCACGGCTTGTAGGCACACGGTTTCAGGTACTATTT<br /> |rRNA<br /> |-<br /> |CAGTCACGACGCAAGGACAAGTCCTTGCGCGACGCTCCCAC<br /> |rRNA<br /> |}</div> RTEvans https://actinobase.org/index.php?title=RRNA_depletion_with_Streptomyces_coelicolor_A3(2)_specific_probes&diff=2181 RRNA depletion with Streptomyces coelicolor A3(2) specific probes 2023-11-01T16:02:21Z <p>RTEvans: /* Materials */</p> <hr /> <div>==Organisms==<br /> <br /> This protocol has been tested with &lt;em&gt;[[Streptomyces coelicolor]]&lt;/em&gt; A3(2) MT1110<br /> <br /> ==Description==<br /> <br /> rRNA depletion using commercial kits targeting generic bacterial sequences is not 100% efficient in the High GC organism S. coelicolor. <br /> <br /> Depletion using the NEBNext RNA Depletion Core Reagent Set with RNA Sample Purification Beads (https://www.neb.com/en-gb/products/e7870-nebnext-rna-depletion-core-reagent-set-with-rna-sample-purification-beads#Product%20Information) and custom DNA probes designed for &lt;em&gt;S. coelicolor&lt;/em&gt; is more effective than the QIAseq FastSelect 5S/16S/23S kit (Qiagen #335927); NEBNext rRNA Depletion Kit (Bacteria) (NEB #E7850); Illumina Ribo-zero rRNA Removal Kit (Bacteria) (Illumina #MRZMB12424, discontinued). The Illumina kit tested was subsequently replaced by the Illumina Ribo-Zero Plus rRNA Depletion Kit (Illumina #20040526). Additionally three abundant non-mRNA RNAs can be depleted (Table 1).<br /> <br /> Table 1. Abundant non-mRNA RNAs in &lt;em&gt;S. coelicolor&lt;/em&gt;.<br /> <br /> [[File: rRNA_depletion_table_1.png]]<br /> <br /> ==Materials==<br /> <br /> Single stranded DNA probes (Table 2) at 2µM concentration in IDTE pH 7.5 (10mM Tris-HCl / 0.1mM EDTA). Integrated DNA Technologies can produce these from their 25nmole DNA oligo pool product. Probes are listed in Table 2 in the Notes section below.<br /> <br /> NEBNext RNA Depletion Core Reagent Set with RNA Sample Purification Beads (https://www.neb.com/en-gb/products/e7870-nebnext-rna-depletion-core-reagent-set-with-rna-sample-purification-beads#Product%20Information).<br /> <br /> ==Protocols==<br /> <br /> As per manufacturer’s instructions.<br /> <br /> ==Notes==<br /> <br /> The following histograms illustrate effectiveness of rRNA depletion using various methods. Histograms show percentage of bases aligned to NCBI reference sequence NC_003888.3 using Hisat2 program classified as follows - Coding = RNA aligned to coding sequences; Transcribed not translated = RNA aligned to rRNA, tRNA and sRNA; Intergenic = RNA aligned to 5' or 3' UTRs or between annotated sequences.<br /> <br /> '''QIAseq FastSelect 5S/16S/23S kit'''<br /> <br /> [[File: PanelA.png]] <br /> <br /> '''NEBNext rRNA Depletion Kit (Bacteria)'''<br /> <br /> [[File: PanelB.png]]<br /> <br /> '''Illumina Ribo-zero rRNA Removal Kit (Bacteria)'''<br /> <br /> [[File: PanelC.png]] <br /> <br /> '''rRNA depletion with &lt;em&gt;S. coelicolor&lt;/em&gt; specific probes<br /> <br /> [[File: PanelD.png]]</div> RTEvans https://actinobase.org/index.php?title=RRNA_depletion_with_Streptomyces_coelicolor_A3(2)_specific_probes&diff=2180 RRNA depletion with Streptomyces coelicolor A3(2) specific probes 2023-11-01T16:01:02Z <p>RTEvans: /* Notes */</p> <hr /> <div>==Organisms==<br /> <br /> This protocol has been tested with &lt;em&gt;[[Streptomyces coelicolor]]&lt;/em&gt; A3(2) MT1110<br /> <br /> ==Description==<br /> <br /> rRNA depletion using commercial kits targeting generic bacterial sequences is not 100% efficient in the High GC organism S. coelicolor. <br /> <br /> Depletion using the NEBNext RNA Depletion Core Reagent Set with RNA Sample Purification Beads (https://www.neb.com/en-gb/products/e7870-nebnext-rna-depletion-core-reagent-set-with-rna-sample-purification-beads#Product%20Information) and custom DNA probes designed for &lt;em&gt;S. coelicolor&lt;/em&gt; is more effective than the QIAseq FastSelect 5S/16S/23S kit (Qiagen #335927); NEBNext rRNA Depletion Kit (Bacteria) (NEB #E7850); Illumina Ribo-zero rRNA Removal Kit (Bacteria) (Illumina #MRZMB12424, discontinued). The Illumina kit tested was subsequently replaced by the Illumina Ribo-Zero Plus rRNA Depletion Kit (Illumina #20040526). Additionally three abundant non-mRNA RNAs can be depleted (Table 1).<br /> <br /> Table 1. Abundant non-mRNA RNAs in &lt;em&gt;S. coelicolor&lt;/em&gt;.<br /> <br /> [[File: rRNA_depletion_table_1.png]]<br /> <br /> ==Materials==<br /> <br /> Single stranded DNA probes (Table 2) at 2µM concentration in IDTE pH 7.5 (10mM Tris-HCl / 0.1mM EDTA). Integrated DNA Technologies can produce these from their 25nmole DNA oligo pool product. <br /> <br /> NEBNext RNA Depletion Core Reagent Set with RNA Sample Purification Beads (https://www.neb.com/en-gb/products/e7870-nebnext-rna-depletion-core-reagent-set-with-rna-sample-purification-beads#Product%20Information).<br /> <br /> ==Protocols==<br /> <br /> As per manufacturer’s instructions.<br /> <br /> ==Notes==<br /> <br /> The following histograms illustrate effectiveness of rRNA depletion using various methods. Histograms show percentage of bases aligned to NCBI reference sequence NC_003888.3 using Hisat2 program classified as follows - Coding = RNA aligned to coding sequences; Transcribed not translated = RNA aligned to rRNA, tRNA and sRNA; Intergenic = RNA aligned to 5' or 3' UTRs or between annotated sequences.<br /> <br /> '''QIAseq FastSelect 5S/16S/23S kit'''<br /> <br /> [[File: PanelA.png]] <br /> <br /> '''NEBNext rRNA Depletion Kit (Bacteria)'''<br /> <br /> [[File: PanelB.png]]<br /> <br /> '''Illumina Ribo-zero rRNA Removal Kit (Bacteria)'''<br /> <br /> [[File: PanelC.png]] <br /> <br /> '''rRNA depletion with &lt;em&gt;S. coelicolor&lt;/em&gt; specific probes<br /> <br /> [[File: PanelD.png]]</div> RTEvans https://actinobase.org/index.php?title=File:PanelD.png&diff=2179 File:PanelD.png 2023-11-01T15:59:35Z <p>RTEvans: RTEvans uploaded a new version of File:PanelD.png</p> <hr /> <div>== Summary ==<br /> Genome mapping of S. coelicolor samples following rRNA depletion using protocol described on this page</div> RTEvans https://actinobase.org/index.php?title=File:PanelC.png&diff=2178 File:PanelC.png 2023-11-01T15:59:17Z <p>RTEvans: RTEvans uploaded a new version of File:PanelC.png</p> <hr /> <div>== Summary ==<br /> Genome mapping of S. coelicolor samples following rRNA depletion using Illumina Ribo-zero rRNA Removal Kit (Bacteria)</div> RTEvans https://actinobase.org/index.php?title=File:PanelA.png&diff=2177 File:PanelA.png 2023-11-01T15:58:53Z <p>RTEvans: RTEvans uploaded a new version of File:PanelA.png</p> <hr /> <div>== Summary ==<br /> Genome mapping of S. coelicolor samples following rRNA depletion with Qiagen FastSelect 5S/16S/23S kit</div> RTEvans https://actinobase.org/index.php?title=File:PanelD.png&diff=2176 File:PanelD.png 2023-11-01T15:56:46Z <p>RTEvans: RTEvans uploaded a new version of File:PanelD.png</p> <hr /> <div>== Summary ==<br /> Genome mapping of S. coelicolor samples following rRNA depletion using protocol described on this page</div> RTEvans https://actinobase.org/index.php?title=File:PanelB.png&diff=2175 File:PanelB.png 2023-11-01T15:52:52Z <p>RTEvans: RTEvans uploaded a new version of File:PanelB.png</p> <hr /> <div>== Summary ==<br /> Genome mapping of S. coelicolor samples following rRNA depletion using NEBNext rRNA Depletion Kit (Bacteria)</div> RTEvans https://actinobase.org/index.php?title=File:PanelA.png&diff=2174 File:PanelA.png 2023-11-01T15:51:04Z <p>RTEvans: RTEvans uploaded a new version of File:PanelA.png</p> <hr /> <div>== Summary ==<br /> Genome mapping of S. coelicolor samples following rRNA depletion with Qiagen FastSelect 5S/16S/23S kit</div> RTEvans https://actinobase.org/index.php?title=File:PanelA.png&diff=2173 File:PanelA.png 2023-11-01T15:49:25Z <p>RTEvans: RTEvans uploaded a new version of File:PanelA.png</p> <hr /> <div>== Summary ==<br /> Genome mapping of S. coelicolor samples following rRNA depletion with Qiagen FastSelect 5S/16S/23S kit</div> RTEvans https://actinobase.org/index.php?title=RRNA_depletion_with_Streptomyces_coelicolor_A3(2)_specific_probes&diff=2172 RRNA depletion with Streptomyces coelicolor A3(2) specific probes 2023-11-01T15:45:50Z <p>RTEvans: /* Notes */</p> <hr /> <div>==Organisms==<br /> <br /> This protocol has been tested with &lt;em&gt;[[Streptomyces coelicolor]]&lt;/em&gt; A3(2) MT1110<br /> <br /> ==Description==<br /> <br /> rRNA depletion using commercial kits targeting generic bacterial sequences is not 100% efficient in the High GC organism S. coelicolor. <br /> <br /> Depletion using the NEBNext RNA Depletion Core Reagent Set with RNA Sample Purification Beads (https://www.neb.com/en-gb/products/e7870-nebnext-rna-depletion-core-reagent-set-with-rna-sample-purification-beads#Product%20Information) and custom DNA probes designed for &lt;em&gt;S. coelicolor&lt;/em&gt; is more effective than the QIAseq FastSelect 5S/16S/23S kit (Qiagen #335927); NEBNext rRNA Depletion Kit (Bacteria) (NEB #E7850); Illumina Ribo-zero rRNA Removal Kit (Bacteria) (Illumina #MRZMB12424, discontinued). The Illumina kit tested was subsequently replaced by the Illumina Ribo-Zero Plus rRNA Depletion Kit (Illumina #20040526). Additionally three abundant non-mRNA RNAs can be depleted (Table 1).<br /> <br /> Table 1. Abundant non-mRNA RNAs in &lt;em&gt;S. coelicolor&lt;/em&gt;.<br /> <br /> [[File: rRNA_depletion_table_1.png]]<br /> <br /> ==Materials==<br /> <br /> Single stranded DNA probes (Table 2) at 2µM concentration in IDTE pH 7.5 (10mM Tris-HCl / 0.1mM EDTA). Integrated DNA Technologies can produce these from their 25nmole DNA oligo pool product. <br /> <br /> NEBNext RNA Depletion Core Reagent Set with RNA Sample Purification Beads (https://www.neb.com/en-gb/products/e7870-nebnext-rna-depletion-core-reagent-set-with-rna-sample-purification-beads#Product%20Information).<br /> <br /> ==Protocols==<br /> <br /> As per manufacturer’s instructions.<br /> <br /> ==Notes==<br /> <br /> The following histograms illustrate effectiveness of rRNA depletion using various methods. Coding = RNA aligned to coding sequences; Transcribed not translated = RNA aligned to rRNA, tRNA and sRNA; Intergenic = RNA aligned to 5' or 3' UTRs or between annotated sequences.<br /> <br /> '''QIAseq FastSelect 5S/16S/23S kit'''<br /> <br /> [[File: PanelA.png]] <br /> <br /> '''NEBNext rRNA Depletion Kit (Bacteria)'''<br /> <br /> [[File: PanelB.png]]<br /> <br /> '''Illumina Ribo-zero rRNA Removal Kit (Bacteria)'''<br /> <br /> [[File: PanelC.png]] <br /> <br /> '''rRNA depletion with &lt;em&gt;S. coelicolor&lt;/em&gt; specific probes<br /> <br /> [[File: PanelD.png]]</div> RTEvans https://actinobase.org/index.php?title=RRNA_depletion_with_Streptomyces_coelicolor_A3(2)_specific_probes&diff=2171 RRNA depletion with Streptomyces coelicolor A3(2) specific probes 2023-11-01T15:39:09Z <p>RTEvans: /* Notes */</p> <hr /> <div>==Organisms==<br /> <br /> This protocol has been tested with &lt;em&gt;[[Streptomyces coelicolor]]&lt;/em&gt; A3(2) MT1110<br /> <br /> ==Description==<br /> <br /> rRNA depletion using commercial kits targeting generic bacterial sequences is not 100% efficient in the High GC organism S. coelicolor. <br /> <br /> Depletion using the NEBNext RNA Depletion Core Reagent Set with RNA Sample Purification Beads (https://www.neb.com/en-gb/products/e7870-nebnext-rna-depletion-core-reagent-set-with-rna-sample-purification-beads#Product%20Information) and custom DNA probes designed for &lt;em&gt;S. coelicolor&lt;/em&gt; is more effective than the QIAseq FastSelect 5S/16S/23S kit (Qiagen #335927); NEBNext rRNA Depletion Kit (Bacteria) (NEB #E7850); Illumina Ribo-zero rRNA Removal Kit (Bacteria) (Illumina #MRZMB12424, discontinued). The Illumina kit tested was subsequently replaced by the Illumina Ribo-Zero Plus rRNA Depletion Kit (Illumina #20040526). Additionally three abundant non-mRNA RNAs can be depleted (Table 1).<br /> <br /> Table 1. Abundant non-mRNA RNAs in &lt;em&gt;S. coelicolor&lt;/em&gt;.<br /> <br /> [[File: rRNA_depletion_table_1.png]]<br /> <br /> ==Materials==<br /> <br /> Single stranded DNA probes (Table 2) at 2µM concentration in IDTE pH 7.5 (10mM Tris-HCl / 0.1mM EDTA). Integrated DNA Technologies can produce these from their 25nmole DNA oligo pool product. <br /> <br /> NEBNext RNA Depletion Core Reagent Set with RNA Sample Purification Beads (https://www.neb.com/en-gb/products/e7870-nebnext-rna-depletion-core-reagent-set-with-rna-sample-purification-beads#Product%20Information).<br /> <br /> ==Protocols==<br /> <br /> As per manufacturer’s instructions.<br /> <br /> ==Notes==<br /> <br /> <br /> A B<br /> <br /> <br /> [[File: PanelA.png]] [[File: PanelB.png]]<br /> <br /> [[File: PanelC.png]] [[File: PanelD.png]]</div> RTEvans https://actinobase.org/index.php?title=RRNA_depletion_with_Streptomyces_coelicolor_A3(2)_specific_probes&diff=2170 RRNA depletion with Streptomyces coelicolor A3(2) specific probes 2023-11-01T15:38:24Z <p>RTEvans: </p> <hr /> <div>==Organisms==<br /> <br /> This protocol has been tested with &lt;em&gt;[[Streptomyces coelicolor]]&lt;/em&gt; A3(2) MT1110<br /> <br /> ==Description==<br /> <br /> rRNA depletion using commercial kits targeting generic bacterial sequences is not 100% efficient in the High GC organism S. coelicolor. <br /> <br /> Depletion using the NEBNext RNA Depletion Core Reagent Set with RNA Sample Purification Beads (https://www.neb.com/en-gb/products/e7870-nebnext-rna-depletion-core-reagent-set-with-rna-sample-purification-beads#Product%20Information) and custom DNA probes designed for &lt;em&gt;S. coelicolor&lt;/em&gt; is more effective than the QIAseq FastSelect 5S/16S/23S kit (Qiagen #335927); NEBNext rRNA Depletion Kit (Bacteria) (NEB #E7850); Illumina Ribo-zero rRNA Removal Kit (Bacteria) (Illumina #MRZMB12424, discontinued). The Illumina kit tested was subsequently replaced by the Illumina Ribo-Zero Plus rRNA Depletion Kit (Illumina #20040526). Additionally three abundant non-mRNA RNAs can be depleted (Table 1).<br /> <br /> Table 1. Abundant non-mRNA RNAs in &lt;em&gt;S. coelicolor&lt;/em&gt;.<br /> <br /> [[File: rRNA_depletion_table_1.png]]<br /> <br /> ==Materials==<br /> <br /> Single stranded DNA probes (Table 2) at 2µM concentration in IDTE pH 7.5 (10mM Tris-HCl / 0.1mM EDTA). Integrated DNA Technologies can produce these from their 25nmole DNA oligo pool product. <br /> <br /> NEBNext RNA Depletion Core Reagent Set with RNA Sample Purification Beads (https://www.neb.com/en-gb/products/e7870-nebnext-rna-depletion-core-reagent-set-with-rna-sample-purification-beads#Product%20Information).<br /> <br /> ==Protocols==<br /> <br /> As per manufacturer’s instructions.<br /> <br /> ==Notes==<br /> <br /> [[File: PanelA.png]] [[File: PanelB.png]]<br /> <br /> [[File: PanelC.png]] [[File: PanelD.png]]</div> RTEvans https://actinobase.org/index.php?title=RRNA_depletion_with_Streptomyces_coelicolor_A3(2)_specific_probes&diff=2169 RRNA depletion with Streptomyces coelicolor A3(2) specific probes 2023-11-01T15:37:58Z <p>RTEvans: </p> <hr /> <div>==Organisms==<br /> <br /> This protocol has been tested with &lt;em&gt;[[Streptomyces coelicolor]]&lt;/em&gt; A3(2) MT1110<br /> <br /> <br /> ==Description==<br /> <br /> rRNA depletion using commercial kits targeting generic bacterial sequences is not 100% efficient in the High GC organism S. coelicolor. <br /> <br /> Depletion using the NEBNext RNA Depletion Core Reagent Set with RNA Sample Purification Beads (https://www.neb.com/en-gb/products/e7870-nebnext-rna-depletion-core-reagent-set-with-rna-sample-purification-beads#Product%20Information) and custom DNA probes designed for &lt;em&gt;S. coelicolor&lt;/em&gt; is more effective than the QIAseq FastSelect 5S/16S/23S kit (Qiagen #335927); NEBNext rRNA Depletion Kit (Bacteria) (NEB #E7850); Illumina Ribo-zero rRNA Removal Kit (Bacteria) (Illumina #MRZMB12424, discontinued). The Illumina kit tested was subsequently replaced by the Illumina Ribo-Zero Plus rRNA Depletion Kit (Illumina #20040526). Additionally three abundant non-mRNA RNAs can be depleted (Table 1).<br /> <br /> Table 1. Abundant non-mRNA RNAs in &lt;em&gt;S. coelicolor&lt;/em&gt;.<br /> <br /> [[File: rRNA_depletion_table_1.png]]<br /> <br /> ==Materials==<br /> <br /> Single stranded DNA probes (Table 2) at 2µM concentration in IDTE pH 7.5 (10mM Tris-HCl / 0.1mM EDTA). Integrated DNA Technologies can produce these from their 25nmole DNA oligo pool product. <br /> <br /> NEBNext RNA Depletion Core Reagent Set with RNA Sample Purification Beads (https://www.neb.com/en-gb/products/e7870-nebnext-rna-depletion-core-reagent-set-with-rna-sample-purification-beads#Product%20Information).<br /> <br /> ==Protocols==<br /> <br /> As per manufacturer’s instructions.<br /> <br /> ==Notes==<br /> <br /> [[File: PanelA.png]] [[File: PanelB.png]]<br /> <br /> [[File: PanelC.png]] [[File: PanelD.png]]</div> RTEvans https://actinobase.org/index.php?title=File:PanelD.png&diff=2168 File:PanelD.png 2023-11-01T15:36:43Z <p>RTEvans: Genome mapping of S. coelicolor samples following rRNA depletion using protocol described on this page</p> <hr /> <div>== Summary ==<br /> Genome mapping of S. coelicolor samples following rRNA depletion using protocol described on this page</div> RTEvans https://actinobase.org/index.php?title=File:PanelC.png&diff=2167 File:PanelC.png 2023-11-01T15:35:49Z <p>RTEvans: Genome mapping of S. coelicolor samples following rRNA depletion using Illumina Ribo-zero rRNA Removal Kit (Bacteria)</p> <hr /> <div>== Summary ==<br /> Genome mapping of S. coelicolor samples following rRNA depletion using Illumina Ribo-zero rRNA Removal Kit (Bacteria)</div> RTEvans https://actinobase.org/index.php?title=File:PanelB.png&diff=2166 File:PanelB.png 2023-11-01T15:35:20Z <p>RTEvans: Genome mapping of S. coelicolor samples following rRNA depletion using NEBNext rRNA Depletion Kit (Bacteria)</p> <hr /> <div>== Summary ==<br /> Genome mapping of S. coelicolor samples following rRNA depletion using NEBNext rRNA Depletion Kit (Bacteria)</div> RTEvans https://actinobase.org/index.php?title=RRNA_depletion_with_Streptomyces_coelicolor_A3(2)_specific_probes&diff=2165 RRNA depletion with Streptomyces coelicolor A3(2) specific probes 2023-11-01T15:34:24Z <p>RTEvans: </p> <hr /> <div>==Organisms==<br /> <br /> This protocol has been tested with &lt;em&gt;[[Streptomyces coelicolor]]&lt;/em&gt; A3(2) MT1110<br /> <br /> <br /> ==Description==<br /> <br /> rRNA depletion using commercial kits targeting generic bacterial sequences is not 100% efficient in the High GC organism S. coelicolor. <br /> <br /> Depletion using the NEBNext RNA Depletion Core Reagent Set with RNA Sample Purification Beads (https://www.neb.com/en-gb/products/e7870-nebnext-rna-depletion-core-reagent-set-with-rna-sample-purification-beads#Product%20Information) and custom DNA probes designed for &lt;em&gt;S. coelicolor&lt;/em&gt; is more effective than the QIAseq FastSelect 5S/16S/23S kit (Qiagen #335927); NEBNext rRNA Depletion Kit (Bacteria) (NEB #E7850); Illumina Ribo-zero rRNA Removal Kit (Bacteria) (Illumina #MRZMB12424, discontinued). The Illumina kit tested was subsequently replaced by the Illumina Ribo-Zero Plus rRNA Depletion Kit (Illumina #20040526). Additionally three abundant non-mRNA RNAs can be depleted (Table 1).<br /> <br /> Table 1. Abundant non-mRNA RNAs in &lt;em&gt;S. coelicolor&lt;/em&gt;.<br /> <br /> [[File: rRNA_depletion_table_1.png]]<br /> <br /> ==Materials==<br /> <br /> Single stranded DNA probes (Table 2) at 2µM concentration in IDTE pH 7.5 (10mM Tris-HCl / 0.1mM EDTA). Integrated DNA Technologies can produce these from their 25nmole DNA oligo pool product. <br /> <br /> NEBNext RNA Depletion Core Reagent Set with RNA Sample Purification Beads (https://www.neb.com/en-gb/products/e7870-nebnext-rna-depletion-core-reagent-set-with-rna-sample-purification-beads#Product%20Information).<br /> <br /> ==Protocols==<br /> <br /> As per manufacturer’s instructions.<br /> <br /> ==Notes==<br /> A B<br /> [[File: PanelA.png]] [[File: PanelB.png]]<br /> <br /> [[File: PanelC.png]] [[File: PanelD.png]]</div> RTEvans https://actinobase.org/index.php?title=File:PanelA.png&diff=2164 File:PanelA.png 2023-11-01T15:33:17Z <p>RTEvans: Genome mapping of S. coelicolor samples following rRNA depletion with Qiagen FastSelect 5S/16S/23S kit</p> <hr /> <div>== Summary ==<br /> Genome mapping of S. coelicolor samples following rRNA depletion with Qiagen FastSelect 5S/16S/23S kit</div> RTEvans https://actinobase.org/index.php?title=RRNA_depletion_with_Streptomyces_coelicolor_A3(2)_specific_probes&diff=2163 RRNA depletion with Streptomyces coelicolor A3(2) specific probes 2023-11-01T15:32:22Z <p>RTEvans: </p> <hr /> <div>==Organisms==<br /> <br /> This protocol has been tested with &lt;em&gt;[[Streptomyces coelicolor]]&lt;/em&gt; A3(2) MT1110<br /> <br /> <br /> ==rRNA depletion with &lt;em&gt;Streptomyces coelicolor&lt;/em&gt; A3(2) specific probes==<br /> <br /> rRNA depletion using commercial kits targeting generic bacterial sequences is not 100% efficient in the High GC organism S. coelicolor. <br /> <br /> Depletion using the NEBNext RNA Depletion Core Reagent Set with RNA Sample Purification Beads (https://www.neb.com/en-gb/products/e7870-nebnext-rna-depletion-core-reagent-set-with-rna-sample-purification-beads#Product%20Information) and custom DNA probes designed for &lt;em&gt;S. coelicolor&lt;/em&gt; is more effective than the QIAseq FastSelect 5S/16S/23S kit (Qiagen #335927); NEBNext rRNA Depletion Kit (Bacteria) (NEB #E7850); Illumina Ribo-zero rRNA Removal Kit (Bacteria) (Illumina #MRZMB12424, discontinued). The Illumina kit tested was subsequently replaced by the Illumina Ribo-Zero Plus rRNA Depletion Kit (Illumina #20040526). Additionally three abundant non-mRNA RNAs can be depleted (Table 1).<br /> <br /> Table 1. Abundant non-mRNA RNAs in &lt;em&gt;S. coelicolor&lt;/em&gt;.<br /> <br /> [[File: rRNA_depletion_table_1.png]]<br /> <br /> ==Materials==<br /> <br /> Single stranded DNA probes (Table 2) at 2µM concentration in IDTE pH 7.5 (10mM Tris-HCl / 0.1mM EDTA). Integrated DNA Technologies can produce these from their 25nmole DNA oligo pool product. <br /> <br /> NEBNext RNA Depletion Core Reagent Set with RNA Sample Purification Beads (https://www.neb.com/en-gb/products/e7870-nebnext-rna-depletion-core-reagent-set-with-rna-sample-purification-beads#Product%20Information).<br /> <br /> ==Protocols==<br /> <br /> As per manufacturer’s instructions.<br /> <br /> ==Notes==<br /> A B<br /> [[File: PanelA.png]] [[File: PanelB.png]]<br /> <br /> [[File: PanelC.png]] [[File: PanelD.png]]</div> RTEvans https://actinobase.org/index.php?title=RRNA_depletion_with_Streptomyces_coelicolor_A3(2)_specific_probes&diff=2162 RRNA depletion with Streptomyces coelicolor A3(2) specific probes 2023-11-01T15:31:16Z <p>RTEvans: /* Notes */</p> <hr /> <div>==Organisms==<br /> <br /> This protocol has been tested with &lt;em&gt;[[Streptomyces coelicolor]]&lt;/em&gt; A3(2) MT1110<br /> <br /> <br /> ==rRNA depletion with &lt;em&gt;Streptomyces coelicolor&lt;/em&gt; A3(2) specific probes==<br /> <br /> rRNA depletion using commercial kits targeting generic bacterial sequences is not 100% efficient in the High GC organism S. coelicolor. <br /> <br /> Depletion using the NEBNext RNA Depletion Core Reagent Set with RNA Sample Purification Beads (https://www.neb.com/en-gb/products/e7870-nebnext-rna-depletion-core-reagent-set-with-rna-sample-purification-beads#Product%20Information) and custom DNA probes designed for &lt;em&gt;S. coelicolor&lt;/em&gt; is more effective than the QIAseq FastSelect 5S/16S/23S kit (Qiagen #335927); NEBNext rRNA Depletion Kit (Bacteria) (NEB #E7850); Illumina Ribo-zero rRNA Removal Kit (Bacteria) (Illumina #MRZMB12424, discontinued). The Illumina kit tested was subsequently replaced by the Illumina Ribo-Zero Plus rRNA Depletion Kit (Illumina #20040526). Additionally three abundant non-mRNA RNAs can be depleted (Table 1).<br /> <br /> Table 1. Abundant non-mRNA RNAs in &lt;em&gt;S. coelicolor&lt;/em&gt;.<br /> <br /> [[File: rRNA_depletion_table_1.png]]<br /> <br /> ==Materials==<br /> <br /> Single stranded DNA probes (Table 2) at 2µM concentration in IDTE pH 7.5 (10mM Tris-HCl / 0.1mM EDTA). Integrated DNA Technologies can produce these from their 25nmole DNA oligo pool product. <br /> <br /> NEBNext RNA Depletion Core Reagent Set with RNA Sample Purification Beads (https://www.neb.com/en-gb/products/e7870-nebnext-rna-depletion-core-reagent-set-with-rna-sample-purification-beads#Product%20Information).<br /> <br /> ==Protocols==<br /> <br /> As per manufacturer’s instructions.<br /> <br /> ==Notes==<br /> <br /> [[File=PanelA.png]] [[File=PanelB.png]]</div> RTEvans https://actinobase.org/index.php?title=RRNA_depletion_with_Streptomyces_coelicolor_A3(2)_specific_probes&diff=2161 RRNA depletion with Streptomyces coelicolor A3(2) specific probes 2023-11-01T15:30:16Z <p>RTEvans: </p> <hr /> <div>==Organisms==<br /> <br /> This protocol has been tested with &lt;em&gt;[[Streptomyces coelicolor]]&lt;/em&gt; A3(2) MT1110<br /> <br /> <br /> ==rRNA depletion with &lt;em&gt;Streptomyces coelicolor&lt;/em&gt; A3(2) specific probes==<br /> <br /> rRNA depletion using commercial kits targeting generic bacterial sequences is not 100% efficient in the High GC organism S. coelicolor. <br /> <br /> Depletion using the NEBNext RNA Depletion Core Reagent Set with RNA Sample Purification Beads (https://www.neb.com/en-gb/products/e7870-nebnext-rna-depletion-core-reagent-set-with-rna-sample-purification-beads#Product%20Information) and custom DNA probes designed for &lt;em&gt;S. coelicolor&lt;/em&gt; is more effective than the QIAseq FastSelect 5S/16S/23S kit (Qiagen #335927); NEBNext rRNA Depletion Kit (Bacteria) (NEB #E7850); Illumina Ribo-zero rRNA Removal Kit (Bacteria) (Illumina #MRZMB12424, discontinued). The Illumina kit tested was subsequently replaced by the Illumina Ribo-Zero Plus rRNA Depletion Kit (Illumina #20040526). Additionally three abundant non-mRNA RNAs can be depleted (Table 1).<br /> <br /> Table 1. Abundant non-mRNA RNAs in &lt;em&gt;S. coelicolor&lt;/em&gt;.<br /> <br /> [[File: rRNA_depletion_table_1.png]]<br /> <br /> ==Materials==<br /> <br /> Single stranded DNA probes (Table 2) at 2µM concentration in IDTE pH 7.5 (10mM Tris-HCl / 0.1mM EDTA). Integrated DNA Technologies can produce these from their 25nmole DNA oligo pool product. <br /> <br /> NEBNext RNA Depletion Core Reagent Set with RNA Sample Purification Beads (https://www.neb.com/en-gb/products/e7870-nebnext-rna-depletion-core-reagent-set-with-rna-sample-purification-beads#Product%20Information).<br /> <br /> ==Protocols==<br /> <br /> As per manufacturer’s instructions.<br /> <br /> ==Notes==</div> RTEvans https://actinobase.org/index.php?title=File:RRNA_depletion_table_1.png&diff=2160 File:RRNA depletion table 1.png 2023-11-01T15:26:14Z <p>RTEvans: Table 1 - list of abundant non mRNA RNAs in S. coelicolor A3(2)</p> <hr /> <div>== Summary ==<br /> Table 1 - list of abundant non mRNA RNAs in S. coelicolor A3(2)</div> RTEvans https://actinobase.org/index.php?title=RRNA_depletion_with_Streptomyces_coelicolor_A3(2)_specific_probes&diff=2159 RRNA depletion with Streptomyces coelicolor A3(2) specific probes 2023-11-01T15:25:13Z <p>RTEvans: Created page with &quot;==Organisms== This protocol has been tested with &lt;em&gt;Streptomyces coelicolor&lt;/em&gt; A3(2) MT1110 ==rRNA depletion with &lt;em&gt;Streptomyces coelicolor&lt;/em&gt; A3(2) specific probes== rRNA depletion using commercial kits targeting generic bacterial sequences is not 100% efficient in the High GC organism S. coelicolor. Depletion using the NEBNext RNA Depletion Core Reagent Set with RNA Sample Purification Beads (https://www.neb.com/en-gb/products/e7870-nebnext-rna-depleti...&quot;</p> <hr /> <div>==Organisms==<br /> <br /> This protocol has been tested with &lt;em&gt;[[Streptomyces coelicolor]]&lt;/em&gt; A3(2) MT1110<br /> <br /> <br /> ==rRNA depletion with &lt;em&gt;Streptomyces coelicolor&lt;/em&gt; A3(2) specific probes==<br /> <br /> rRNA depletion using commercial kits targeting generic bacterial sequences is not 100% efficient in the High GC organism S. coelicolor. <br /> <br /> Depletion using the NEBNext RNA Depletion Core Reagent Set with RNA Sample Purification Beads (https://www.neb.com/en-gb/products/e7870-nebnext-rna-depletion-core-reagent-set-with-rna-sample-purification-beads#Product%20Information) and custom DNA probes designed for &lt;em&gt;S. coelicolor&lt;/em&gt; is more effective than the QIAseq FastSelect 5S/16S/23S kit (Qiagen #335927); NEBNext rRNA Depletion Kit (Bacteria) (NEB #E7850); Illumina Ribo-zero rRNA Removal Kit (Bacteria) (Illumina #MRZMB12424, discontinued). The Illumina kit tested was subsequently replaced by the Illumina Ribo-Zero Plus rRNA Depletion Kit (Illumina #20040526). Additionally three abundant non-mRNA RNAs can be depleted (Table 1).<br /> <br /> Table 1 Abundant non mRNA RNAs in S. coelicolor.<br /> [[File: rRNA_depletion_table_1.png]]</div> RTEvans https://actinobase.org/index.php?title=Protocols&diff=2158 Protocols 2023-11-01T15:14:17Z <p>RTEvans: /* Next Generation Sequencing */</p> <hr /> <div>[[File:Actinobase-icons-trans_Protocols.png|150px|Protocols|link=Protocols]] <br /> <br /> =Protocols organised by category=<br /> <br /> ==[[Bacteriophage]]==<br /> <br /> *[[Streptomyces Bacteriophage plaque assay]]<br /> <br /> *[[High titre preparation of phage]]<br /> <br /> *[[Isolation of phages from single plaques]]<br /> <br /> *[[Isolation of phages from environmental samples]]<br /> <br /> ==[[Biochemistry]]==<br /> <br /> *[[GUS assay]] (β-Glucuronidase assay)<br /> <br /> ==Microbiology==<br /> <br /> *[[Characterisation of Actinomycetes | General Characterisaton of Actinomycetes]]<br /> <br /> *[[Conjugation using ET12567/pUZ8002]]<br /> <br /> *[[Exploration | ''Streptomyces'' Exploration]]<br /> <br /> *[[General Growth and Maintenance of Streptomyces sp.]]<br /> <br /> *[[Isolating Actinomycetes from the environment]]<br /> <br /> For a comprehensive guide to actinomycete characterisation see the [https://www.dsmz.de/collection/catalogue/microorganisms/special-groups-of-organisms/compendium-of-actinobacteria Compendium of Actinobacteria] from [https://www.helmholtz-hzi.de/en/research/research-topics/anti-infectives/microbial-strain-collection/our-research/#anchorsection Prof. Joachim Wink] at the German Collection of Microorganisms &amp; Cell Cultures ([https://www.dsmz.de/ DSMZ]). This resouce provides protocols for characterising a wide range of features for new isolates, but may also be applicable more broadly for example for phenotyping experiments. The tests include colony morphology, melanin production, salt tolerance, carbon utlilisation and much more. To download the guide please see the link below. <br /> <br /> [https://actinobase.org/downloads/DSMZ.pdf DSMZ Compendium of Actinobacteria]<br /> <br /> ==[[Plant-Microbe interactions]]==<br /> <br /> * See [[Plant-Microbe interactions|main section]] for full list of protocols<br /> <br /> ==[[Microscopy]]==<br /> <br /> *[[Staining of ''Streptomyces'' spp. Cell Wall and Nucleic Acids| Staining of ''Streptomyces'' spp. Cell Wall and Nucleic Acids]]<br /> <br /> *[[Cultivating Actinobacteria for Microscopic Analysis]]<br /> <br /> ==[[Mutagenesis]]==<br /> <br /> *[[CRISPR/Cas9-mediated genome editing]]<br /> <br /> *[[Chemical mutagenesis]]<br /> <br /> *[[Depletion strains]]<br /> <br /> *[[Lambda-red mediated recombination (PCR-targeting system a.k.a. &quot;Redirect&quot;)]]<br /> <br /> *[[Lambda-red mediated recombination using ssDNA]]<br /> <br /> *[[Meganuclease I-SceI based system for gene deletions]]<br /> <br /> *[[Protoplasts Formation, Regeneration and Transformation]]<br /> <br /> *[[&quot;Quikchange&quot; site-directed mutagenesis]]<br /> <br /> *[[Random mutagenesis using a mutator strain]]<br /> <br /> *[[Random mutagenesis using error-prone PCR]]<br /> <br /> *[[Suicide vectors]]<br /> <br /> *[[Transposons]]<br /> <br /> *[[UV mutagenesis]]<br /> <br /> ==[[Natural Products]]== <br /> <br /> *[[Cloning, Transformation and Expression of Gene Clusters]]<br /> <br /> *[[Metabolite extractions]]<br /> <br /> *[[Production of microbial volatile organic compounds]]<br /> <br /> ==[[Next Generation Sequencing]]==<br /> <br /> *[[ChIP Sequencing]]<br /> *[[ChIP-exo sequencing]]<br /> https://www.nature.com/articles/s41467-018-05265-7/<br /> *[[RNA Sequencing]]<br /> *[[rRNA depletion with Streptomyces coelicolor A3(2) specific probes]]<br /> *[[Differential RNA sequencing]]<br /> <br /> ==[[Nucleic Acid Methods]]==<br /> <br /> *[[Codon Optimising Sequences for Heterologous Expression in Streptomyces]]<br /> <br /> *[[High GC PCR]]<br /> <br /> *[[RNA extraction from Actinobacteria]]<br /> <br /> *[[RNA interference]]<br /> <br /> *[[Salting Out Genomic DNA Extraction Method]]<br /> <br /> *[[Southern Blotting]]<br /> <br /> ==[[Protein Methods]]==<br /> <br /> *[[Isolating extracellular protein from Streptomyces]]<br /> <br /> =Alphabetical list of protocols=<br /> <br /> [[Characterisation of Actinomycetes]]<br /> <br /> [[Cloning, Transformation and Expression of Gene Clusters]]<br /> <br /> [[CRISPR/Cas9-mediated genome editing]]<br /> <br /> [[Chemical mutagenesis]]<br /> <br /> [[ChIP Seq]]<br /> <br /> [[Codon Optimising Sequences for Heterologous Expression in Streptomyces]]<br /> <br /> [[Conjugation using ET12567/pUZ8002]]<br /> <br /> [[Depletion strains]]<br /> <br /> [[Exploration | ''Streptomyces'' Exploration]]<br /> <br /> [[General Growth and Maintenance of Streptomyces sp.]]<br /> <br /> [[GUS assay]] (β-Glucuronidase assay)<br /> <br /> [[High GC PCR]]<br /> <br /> [[High titre preparation of phage]]<br /> <br /> [[Isolating Actinomycetes from the environment]]<br /> <br /> [[Isolating extracellular protein from Streptomyces]]<br /> <br /> [[Isolating Streptomyces from plant roots]]<br /> <br /> [[Isolation of phages from environmental samples]]<br /> <br /> [[Isolation of phages from single plaques]]<br /> <br /> [[Lambda-red mediated recombination (PCR-targeting system a.k.a. &quot;Redirect&quot;)]]<br /> <br /> [[Lambda-red mediated recombination using ssDNA]]<br /> <br /> [[Meganuclease I-SceI based system for gene deletions]]<br /> <br /> [[Metabolite extractions]]<br /> <br /> [[Protoplasts Formation, Regeneration and Transformation]]<br /> <br /> [[&quot;Quikchange&quot; site-directed mutagenesis]]<br /> <br /> [[Random mutagenesis using a mutator strain]]<br /> <br /> [[Random mutagenesis using error-prone PCR]]<br /> <br /> [[RNA extraction from Actinobacteria]]<br /> <br /> [[Salting Out Genomic DNA Extraction Method]]<br /> <br /> [[Southern Blotting]]<br /> <br /> [[Staining of ''Streptomyces'' spp. Cell Wall and Nucleic Acids| Staining of ''Streptomyces'' spp. Cell Wall and Nucleic Acids]]<br /> <br /> [[Streptomyces Bacteriophage plaque assay]]<br /> <br /> [[Suicide vectors]]<br /> <br /> [[Transposons]]<br /> <br /> [[UV mutagenesis]]</div> RTEvans https://actinobase.org/index.php?title=Protocols&diff=2157 Protocols 2023-11-01T15:13:33Z <p>RTEvans: /* Next Generation Sequencing */</p> <hr /> <div>[[File:Actinobase-icons-trans_Protocols.png|150px|Protocols|link=Protocols]] <br /> <br /> =Protocols organised by category=<br /> <br /> ==[[Bacteriophage]]==<br /> <br /> *[[Streptomyces Bacteriophage plaque assay]]<br /> <br /> *[[High titre preparation of phage]]<br /> <br /> *[[Isolation of phages from single plaques]]<br /> <br /> *[[Isolation of phages from environmental samples]]<br /> <br /> ==[[Biochemistry]]==<br /> <br /> *[[GUS assay]] (β-Glucuronidase assay)<br /> <br /> ==Microbiology==<br /> <br /> *[[Characterisation of Actinomycetes | General Characterisaton of Actinomycetes]]<br /> <br /> *[[Conjugation using ET12567/pUZ8002]]<br /> <br /> *[[Exploration | ''Streptomyces'' Exploration]]<br /> <br /> *[[General Growth and Maintenance of Streptomyces sp.]]<br /> <br /> *[[Isolating Actinomycetes from the environment]]<br /> <br /> For a comprehensive guide to actinomycete characterisation see the [https://www.dsmz.de/collection/catalogue/microorganisms/special-groups-of-organisms/compendium-of-actinobacteria Compendium of Actinobacteria] from [https://www.helmholtz-hzi.de/en/research/research-topics/anti-infectives/microbial-strain-collection/our-research/#anchorsection Prof. Joachim Wink] at the German Collection of Microorganisms &amp; Cell Cultures ([https://www.dsmz.de/ DSMZ]). This resouce provides protocols for characterising a wide range of features for new isolates, but may also be applicable more broadly for example for phenotyping experiments. The tests include colony morphology, melanin production, salt tolerance, carbon utlilisation and much more. To download the guide please see the link below. <br /> <br /> [https://actinobase.org/downloads/DSMZ.pdf DSMZ Compendium of Actinobacteria]<br /> <br /> ==[[Plant-Microbe interactions]]==<br /> <br /> * See [[Plant-Microbe interactions|main section]] for full list of protocols<br /> <br /> ==[[Microscopy]]==<br /> <br /> *[[Staining of ''Streptomyces'' spp. Cell Wall and Nucleic Acids| Staining of ''Streptomyces'' spp. Cell Wall and Nucleic Acids]]<br /> <br /> *[[Cultivating Actinobacteria for Microscopic Analysis]]<br /> <br /> ==[[Mutagenesis]]==<br /> <br /> *[[CRISPR/Cas9-mediated genome editing]]<br /> <br /> *[[Chemical mutagenesis]]<br /> <br /> *[[Depletion strains]]<br /> <br /> *[[Lambda-red mediated recombination (PCR-targeting system a.k.a. &quot;Redirect&quot;)]]<br /> <br /> *[[Lambda-red mediated recombination using ssDNA]]<br /> <br /> *[[Meganuclease I-SceI based system for gene deletions]]<br /> <br /> *[[Protoplasts Formation, Regeneration and Transformation]]<br /> <br /> *[[&quot;Quikchange&quot; site-directed mutagenesis]]<br /> <br /> *[[Random mutagenesis using a mutator strain]]<br /> <br /> *[[Random mutagenesis using error-prone PCR]]<br /> <br /> *[[Suicide vectors]]<br /> <br /> *[[Transposons]]<br /> <br /> *[[UV mutagenesis]]<br /> <br /> ==[[Natural Products]]== <br /> <br /> *[[Cloning, Transformation and Expression of Gene Clusters]]<br /> <br /> *[[Metabolite extractions]]<br /> <br /> *[[Production of microbial volatile organic compounds]]<br /> <br /> ==[[Next Generation Sequencing]]==<br /> <br /> *[[ChIP Sequencing]]<br /> *[[ChIP-exo sequencing]]<br /> https://www.nature.com/articles/s41467-018-05265-7/<br /> *[[RNA Sequencing]]<br /> *[[rRNA depletion with &lt;I&gt;Streptomyces coelicolor A3(2)&lt;/I&gt; specific probes]]<br /> *[[Differential RNA sequencing]]<br /> <br /> ==[[Nucleic Acid Methods]]==<br /> <br /> *[[Codon Optimising Sequences for Heterologous Expression in Streptomyces]]<br /> <br /> *[[High GC PCR]]<br /> <br /> *[[RNA extraction from Actinobacteria]]<br /> <br /> *[[RNA interference]]<br /> <br /> *[[Salting Out Genomic DNA Extraction Method]]<br /> <br /> *[[Southern Blotting]]<br /> <br /> ==[[Protein Methods]]==<br /> <br /> *[[Isolating extracellular protein from Streptomyces]]<br /> <br /> =Alphabetical list of protocols=<br /> <br /> [[Characterisation of Actinomycetes]]<br /> <br /> [[Cloning, Transformation and Expression of Gene Clusters]]<br /> <br /> [[CRISPR/Cas9-mediated genome editing]]<br /> <br /> [[Chemical mutagenesis]]<br /> <br /> [[ChIP Seq]]<br /> <br /> [[Codon Optimising Sequences for Heterologous Expression in Streptomyces]]<br /> <br /> [[Conjugation using ET12567/pUZ8002]]<br /> <br /> [[Depletion strains]]<br /> <br /> [[Exploration | ''Streptomyces'' Exploration]]<br /> <br /> [[General Growth and Maintenance of Streptomyces sp.]]<br /> <br /> [[GUS assay]] (β-Glucuronidase assay)<br /> <br /> [[High GC PCR]]<br /> <br /> [[High titre preparation of phage]]<br /> <br /> [[Isolating Actinomycetes from the environment]]<br /> <br /> [[Isolating extracellular protein from Streptomyces]]<br /> <br /> [[Isolating Streptomyces from plant roots]]<br /> <br /> [[Isolation of phages from environmental samples]]<br /> <br /> [[Isolation of phages from single plaques]]<br /> <br /> [[Lambda-red mediated recombination (PCR-targeting system a.k.a. &quot;Redirect&quot;)]]<br /> <br /> [[Lambda-red mediated recombination using ssDNA]]<br /> <br /> [[Meganuclease I-SceI based system for gene deletions]]<br /> <br /> [[Metabolite extractions]]<br /> <br /> [[Protoplasts Formation, Regeneration and Transformation]]<br /> <br /> [[&quot;Quikchange&quot; site-directed mutagenesis]]<br /> <br /> [[Random mutagenesis using a mutator strain]]<br /> <br /> [[Random mutagenesis using error-prone PCR]]<br /> <br /> [[RNA extraction from Actinobacteria]]<br /> <br /> [[Salting Out Genomic DNA Extraction Method]]<br /> <br /> [[Southern Blotting]]<br /> <br /> [[Staining of ''Streptomyces'' spp. Cell Wall and Nucleic Acids| Staining of ''Streptomyces'' spp. Cell Wall and Nucleic Acids]]<br /> <br /> [[Streptomyces Bacteriophage plaque assay]]<br /> <br /> [[Suicide vectors]]<br /> <br /> [[Transposons]]<br /> <br /> [[UV mutagenesis]]</div> RTEvans