ActinoBase - User contributions [en-gb]

High GC PCR

2019-07-04T16:04:13Z

Test:

=High GC PCR=
Actinobacteria typically have a high GC content in their DNA. High GC DNA templates can be difficult to amplify by PCR because of stronger hydrogen bonding and possible formation of secondary structures.

==Tips for successful high GC PCR==

*Additives such as dimethyl sulfoxide (DMSO) can be added to reaction mixtures up to a final concentration of 1-10% can help denature DNA and promote specificity.

*When designing primers, assess properties such as melting temperature and the likelihood of secondary structure formation. A useful tool for this is Net Primer (http://www.premierbiosoft.com/netprimer/).

*When amplifying a region of DNA, try to find small regions of higher AT content that could form part of the primer sequence. This well help lower the Tm of the primers and reduce self-annealing and secondary structure formation.

*Some specific polymerases can aid high GC DNA amplification. High fidelity polymerases will help improve specificity.

*Q5® High-Fidelity DNA Polymerase comes with a high GC enhancer that can be added to reaction mixtures up to a final concentration of 10-20% to improve specificity.

==General protocol for ''Streptomyces'' colony PCR==
#Streak your strain of interest for single colonies on [[DNA]].
#Pick a single colony and crush in 100;micro&l 50% DMSO.
#Heat at 65 degrees for 20 minutes.
#Add 1;micro&l of this to 10;micro&l of PCR mix

High GC PCR

2019-07-04T16:03:20Z

Test: /* How to:Streptomyces colony PCR */

=High GC PCR=
Actinobacteria typically have a high GC content in their DNA. High GC DNA templates can be difficult to amplify by PCR because of stronger hydrogen bonding and possible formation of secondary structures.

==Tips for successful high GC PCR==

*Additives such as dimethyl sulfoxide (DMSO) can be added to reaction mixtures up to a final concentration of 1-10% can help denature DNA and promote specificity.

*When designing primers, assess properties such as melting temperature and the likelihood of secondary structure formation. A useful tool for this is Net Primer (http://www.premierbiosoft.com/netprimer/).

*When amplifying a region of DNA, try to find small regions of higher AT content that could form part of the primer sequence. This well help lower the Tm of the primers and reduce self-annealing and secondary structure formation.

*Some specific polymerases can aid high GC DNA amplification. High fidelity polymerases will help improve specificity.

*Q5® High-Fidelity DNA Polymerase comes with a high GC enhancer that can be added to reaction mixtures up to a final concentration of 10-20% to improve specificity.

==How to: ''Streptomyces'' colony PCR==
#Streak your strain of interest for single colonies on [[DNA]].
#Pick a single colony and crush in 100;micro&l 50% DMSO.
#Heat at 65 degrees for 20 minutes.
#Add 1;micro&l of this to 10;micro&l of PCR mix

High GC PCR

2019-07-04T16:03:08Z

Test:

=High GC PCR=
Actinobacteria typically have a high GC content in their DNA. High GC DNA templates can be difficult to amplify by PCR because of stronger hydrogen bonding and possible formation of secondary structures.

==Tips for successful high GC PCR==

*Additives such as dimethyl sulfoxide (DMSO) can be added to reaction mixtures up to a final concentration of 1-10% can help denature DNA and promote specificity.

*When designing primers, assess properties such as melting temperature and the likelihood of secondary structure formation. A useful tool for this is Net Primer (http://www.premierbiosoft.com/netprimer/).

*When amplifying a region of DNA, try to find small regions of higher AT content that could form part of the primer sequence. This well help lower the Tm of the primers and reduce self-annealing and secondary structure formation.

*Some specific polymerases can aid high GC DNA amplification. High fidelity polymerases will help improve specificity.

*Q5® High-Fidelity DNA Polymerase comes with a high GC enhancer that can be added to reaction mixtures up to a final concentration of 10-20% to improve specificity.

==How to:''Streptomyces'' colony PCR==
#Streak your strain of interest for single colonies on [[DNA]].
#Pick a single colony and crush in 100;micro&l 50% DMSO.
#Heat at 65 degrees for 20 minutes.
#Add 1;micro&l of this to 10;micro&l of PCR mix

High GC PCR

2019-07-04T16:01:37Z

Test:

High GC PCR

2019-07-04T16:00:51Z

Test:

Actinobacteria typically have a high GC content in their DNA. High GC DNA templates can be difficult to amplify by PCR because of stronger hydrogen bonding and possible formation of secondary structures.

==Tips for successful high GC PCR:==

*Additives such as dimethyl sulfoxide (DMSO) can be added to reaction mixtures up to a final concentration of 1-10% can help denature DNA and promote specificity.

*When designing primers, assess properties such as melting temperature and the likelihood of secondary structure formation. A useful tool for this is Net Primer (http://www.premierbiosoft.com/netprimer/).

*When amplifying a region of DNA, try to find small regions of higher AT content that could form part of the primer sequence. This well help lower the Tm of the primers and reduce self-annealing and secondary structure formation.

*Some specific polymerases can aid high GC DNA amplification. High fidelity polymerases will help improve specificity.

*Q5® High-Fidelity DNA Polymerase comes with a high GC enhancer that can be added to reaction mixtures up to a final concentration of 10-20% to improve specificity.

==General protocol for ''Streptomyces'' colony PCR==
#Streak your strain of interest for single colonies on [[DNA]].
#Pick a single colony and crush in 100;micro&l 50% DMSO.
#Heat at 65 degrees for 20 minutes.
#Add 1;micro&l of this to 10;micro&l of PCR mix

DNA

2019-07-04T15:59:23Z

Test:

=Difco Nutrient Agar (DNA)=

Difco Nutrient Agar is a rich medium commercially available from BD and routinely used for cultivating many non-fastidious organisms. It contains beef extract and peptones; exact composition will vary between batches.

Many Streptomyces sp. grow well on DNA, but developmental and/or antibiotic production phenotypes may differ compared to growth on other media (e.g. [[SFM]]).

==Preparation==
Per Litre:
*4g Difco Nutrient Broth Powder
*10g Agar
*1L dH2O

'''Instructions'''
#For solid medium, add 0.8g of DNB powder into each 250ml conical flask
#Add 2g of Agar
#Add 200ml dH2O
#Autoclave

==Uses==
*''Streptomyces'' Colony PCR (see [[High GC PCR]] for instructions)
*[[Spore titres]]
*[[Re-isolation experiments]]
*[[Selection for resistance to salt-sensitive antibiotics]]

==Notes==
For growth in liquid culture, [[DNB]] can be made without the agar.

==References==

High GC PCR

2019-07-04T15:58:15Z

Test:

Actinobacteria typically have a high GC content in their DNA. High GC DNA templates can be difficult to amplify by PCR because of stronger hydrogen bonding and possible formation of secondary structures.

Tips for successful high GC PCR:

*Additives such as dimethyl sulfoxide (DMSO) can be added to reaction mixtures up to a final concentration of 1-10% can help denature DNA and promote specificity.

*When designing primers, assess properties such as melting temperature and the likelihood of secondary structure formation. A useful tool for this is Net Primer (http://www.premierbiosoft.com/netprimer/).

*When amplifying a region of DNA, try to find small regions of higher AT content that could form part of the primer sequence. This well help lower the Tm of the primers and reduce self-annealing and secondary structure formation.

*Some specific polymerases can aid high GC DNA amplification. High fidelity polymerases will help improve specificity.

*Q5® High-Fidelity DNA Polymerase comes with a high GC enhancer that can be added to reaction mixtures up to a final concentration of 10-20% to improve specificity.

General protocol for ''Streptomyces'' colony PCR
#Streak your strain of interest for single colonies on [[DNA]].
#Pick a single colony and crush in 100;micro&l 50% DMSO.
#Heat at 65 degrees for 20 minutes.
#Add 1;micro&l of this to 10;micro&l of PCR mix

Protocols

2019-07-04T15:53:30Z

Test:

[[File:Actinobase-icons-trans_Protocols.png|150px|Protocols|link=Protocols]]

[[General Growth and Maintenance of Streptomyces sp.]]

[[Mutagenesis]]

[[Nucleic Acid Methods]]

[[Protein Methods]]

[[High GC PCR]]

[[Cloning and Expression of Gene Clusters]]

PCR

2019-07-04T15:53:17Z

Test: Blanked the page

MM

2019-07-04T15:48:51Z

Test:

=MM (Minimal Medium)=
MM is a minimal medium suitable for growth of many Streptomyces sp (S. coelicolor and S. venezuelae both grow well on it). It is often made up with glucose or mannitol, but other carbon sources can be used. It can be further supplemented with additional growth factors as described below.

==Preparation==
Per litre:
*0.5g L-asparagine
*0.5g K2HPO4
*0.2g MgSO4*7H2O
*0.01g FeSO4*7H2O
*10g glucose (added after autoclaving)
*10g agar
*1L dH2O

'''Instructions'''
#Dissolve all of the ingredients except the agar and the glucose in the distilled water
#Adjust the pH to 7.0-7.2
#Dispense 200ml into flasks each containing 2g agar
#Autoclave
#After autoclaving, add 4 ml sterile 50% glucose solution (autoclaved separately or filter-sterilized) to each flask
#Add any other growth factors required for auxotrophic mutants

==Uses==
*Studies of auxotrophic mutants and carbon source utilization.

==Notes==
The glucose can be replaced by alternative carbon sources such as glycerol, arabinose, or mannitol (each at 5g/L). Mannitol is often preferable to glucose for optimal growth and development.

To study carbon source utilization, the L-asparagine should be replaced by ammonium sulfate (5 g/L(NH4)2SO4) - to avoid confusion due to utilization of asparagine as a carbon source.

Additional supplements can be added as follows (amount/L): amino acids except histidine, 50 mg; histidine, 70 mg; adenine and uracil, 10 mg; vitamins, 1 mg; dihydrostreptomycin sulfate, 50 mg; acriflavine, 15 mg. (Hopwood 1967)

==References==
Hopwood DA. Genetic analysis and genome structure in Streptomyces coelicolor. Bacteriol Rev. 1967;31(4):373–403.

Kieser, T., Bibb, M.J., Buttner, M.J., Chater, K.F. and Hopwood, D.A. (2000) Practical Streptomyces Genetics: John Innes Foundation, Norwich Research Park, Colney, Norwich NR4 7UH, UK

MYM

2019-07-04T15:47:10Z

Test:

=MYM Medium=

Maltose-Yeast Extract-Malt Extract (MYM) medium is a rich medium used especially for the growth of ''[[Streptomyces venezuelae]]'', as it sporulates to >90% completion in liquid MYM [2]. Other Streptomyces sp. may also grow well on this medium.

==Preparation==
Per litre:
*4g Maltose
*4g Yeast Extract Oxoid
*10g Malt Extract
*500ml Tap Water
*500ml dH2O

'''Instructions'''

#Dissolve all ingredients in 500ml tap water & 250ml dH2O
#pH to 7.2-7.3 with NaOH
#add dH2O up to 1L
#For solid medium, add 2g agar & 100mL liquid MYM to each flask
#Autoclave, expect a precipitate to form afterward.
#For optimal ''[[Streptomyces venezuelae]]'' sporulation, add [[trace elements]] at a 1:500 dilution.

==Uses==
*Routine growth of ''[[Streptomyces venezuelae]]''
*Developmental studies on ''[[Streptomyces venezuelae]]''

==Notes==
If using for growth measurements in liquid medium, it is best to allow the precipitate to settle overnight before pipetting the medium into growth vessels - take from the top to avoid the precipitate.

==References==
1. Stuttard C. 1982. Temperate phages of Streptomyces venezuelae: lysogeny and host specificity shown by phages SV1 and SV2. Microbiology 128:115–121.

2. Bibb MJ, Domonkos A, Chandra G, Buttner MJ. 2012. Expression of the chaplin and rodlin hydrophobic sheath proteins in Streptomyces venezuelae is controlled by σBldN and a cognate anti-sigma factor, RsbN. Mol. Microbiol. 84:1033–1049

MYM

2019-07-04T15:46:27Z

Test:

=MYM Medium=

Maltose-Yeast Extract-Malt Extract (MYM) medium is a rich medium used especially for the growth of ''[[Streptomyces venezuelae]]'', as it sporulates to >90% completion in liquid MYM [1]. Other Streptomyces sp. may also grow well on this medium.

==Preparation==
Per litre:
*4g Maltose
*4g Yeast Extract Oxoid
*10g Malt Extract
*500ml Tap Water
*500ml dH2O

'''Instructions'''

#Dissolve all ingredients in 500ml tap water & 250ml dH2O
#pH to 7.2-7.3 with NaOH
#add dH2O up to 1L
#For solid medium, add 2g agar & 100mL liquid MYM to each flask
#Autoclave, expect a precipitate to form afterward.
#For optimal ''[[Streptomyces venezuelae]]'' sporulation, add [[trace elements]] at a 1:500 dilution.

==Uses==
*Routine growth of ''[[Streptomyces venezuelae]]''
*Developmental studies on ''[[Streptomyces venezuelae]]''

==Notes==
If using for growth measurements in liquid medium, it is best to allow the precipitate to settle overnight before pipetting the medium into growth vessels - take from the top to avoid the precipitate.

==References==
Stuttard C. 1982. Temperate phages of Streptomyces venezuelae: lysogeny and host specificity shown by phages SV1 and SV2. Microbiology 128:115–121.

1. Bibb MJ, Domonkos A, Chandra G, Buttner MJ. 2012. Expression of the chaplin and rodlin hydrophobic sheath proteins in Streptomyces venezuelae is controlled by σBldN and a cognate anti-sigma factor, RsbN. Mol. Microbiol. 84:1033–1049

MYM

2019-07-04T15:44:38Z

Test:

=MYM Medium=

Maltose-Yeast Extract-Malt Extract (MYM) medium is a rich medium used especially for the growth of ''[[Streptomyces venezuelae]]'', as it sporulates to >90% completion in liquid MYM (Bibb et al 2012). Other Streptomyces sp. may also grow well on this medium.

==Preparation==
Per litre:
*4g Maltose
*4g Yeast Extract Oxoid
*10g Malt Extract
*500ml Tap Water
*500ml dH2O

'''Instructions'''

#Dissolve all ingredients in 500ml tap water & 250ml dH2O
#pH to 7.2-7.3 with NaOH
#add dH2O up to 1L
#For solid medium, add 2g agar & 100mL liquid MYM to each flask
#Autoclave, expect a precipitate to form afterward.
#For optimal ''[[Streptomyces venezuelae]]'' sporulation, add [[trace elements]] at a 1:500 dilution.

==Uses==
*Routine growth of ''[[Streptomyces venezuelae]]''
*Developmental studies on ''[[Streptomyces venezuelae]]''

==Notes==
If using for growth measurements in liquid medium, it is best to allow the precipitate to settle overnight before pipetting the medium into growth vessels - take from the top to avoid the precipitate.

==References==
Stuttard C. 1982. Temperate phages of Streptomyces venezuelae: lysogeny and host specificity shown by phages SV1 and SV2. Microbiology 128:115–121.

Bibb MJ, Domonkos A, Chandra G, Buttner MJ. 2012. Expression of the chaplin and rodlin hydrophobic sheath proteins in Streptomyces venezuelae is controlled by σBldN and a cognate anti-sigma factor, RsbN. Mol. Microbiol. 84:1033–1049

Nucleic Acid Methods

2019-07-04T15:25:19Z

Test: Created page with "ChIP Seq"

[[ChIP Seq]]

Main Page

2019-05-30T11:57:59Z

Test: /* Site currently under construction! */

<h2>ActinoBase: a Streptomyces Community Wiki</h2>

===Message from the developers- Site currently under construction!===
----
ActinoBase is a community resource, available to anyone interested studying Actinobacteria! This wiki is being created by the community and for the community to share knowledge and resources pertaining to the study of Actinomycetes such as Streptomyces.

When complete ActinoBase will provide media recipes, experimental protocols, plasmid maps and links to other useful resources. This database will be curated by the Streptomyces scientific community to ensure this resource is accurate and up to date. We hope ActinoBase will not only mediate the sharing of scientific resources amongst the existing Streptomyces community, but make accessible invaluable resources for those looking to begin studying the biology of Actinobacteria. Streptomyces bacteria have the potential to contribute solutions to global issues such as antibiotic resistance & food security through their prolific production of antibiotics and relationship with plant roots. This makes Streptomyces biology an important system to study as we seek to address these issues.

Over the coming months we will be redesigning the site layout and adding useful content, please follow us on twitter [https://twitter.com/ActinoBase @ActinoBase] or join the discussion via [https://twitter.com/hashtag/ActinoBase?src=hash #ActinoBase] to keep up to date with the our progresses! If you have any questions or requests you can email the ActinoBase committee overseeing the construction of this website at teamwikimyces@gmail.com

Updated- 30.05.2019
----

 Useful external links.

* [https://twitter.com/ActinoBase Twitter @ActinoBase]
* [http://streptomyces.org.uk Additional Streptomyces resources including the StrepDB genome database]

Recipes for buffers and growth media:

[[Media recipes]]

Protocols for purifying nucleic acids:

[[RNA preps]]

[[DNA preps]]

Main Page

2019-05-30T11:57:18Z

Test:

<h2>ActinoBase: a Streptomyces Community Wiki</h2>

===Site currently under construction!===
----
ActinoBase is a community resource, available to anyone interested studying Actinobacteria! This wiki is being created by the community and for the community to share knowledge and resources pertaining to the study of Actinomycetes such as Streptomyces.

When complete ActinoBase will provide media recipes, experimental protocols, plasmid maps and links to other useful resources. This database will be curated by the Streptomyces scientific community to ensure this resource is accurate and up to date. We hope ActinoBase will not only mediate the sharing of scientific resources amongst the existing Streptomyces community, but make accessible invaluable resources for those looking to begin studying the biology of Actinobacteria. Streptomyces bacteria have the potential to contribute solutions to global issues such as antibiotic resistance & food security through their prolific production of antibiotics and relationship with plant roots. This makes Streptomyces biology an important system to study as we seek to address these issues.

Over the coming months we will be redesigning the site layout and adding useful content, please follow us on twitter [https://twitter.com/ActinoBase @ActinoBase] or join the discussion via [https://twitter.com/hashtag/ActinoBase?src=hash #ActinoBase] to keep up to date with the our progresses! If you have any questions or requests you can email the ActinoBase committee overseeing the construction of this website at teamwikimyces@gmail.com

Updated- 30.05.2019
----

 Useful external links.

* [https://twitter.com/ActinoBase Twitter @ActinoBase]
* [http://streptomyces.org.uk Additional Streptomyces resources including the StrepDB genome database]

Recipes for buffers and growth media:

[[Media recipes]]

Protocols for purifying nucleic acids:

[[RNA preps]]

[[DNA preps]]

Media recipes

2019-05-30T11:56:36Z

Test:

[[SFM]] (Soya Flour + Mannitol agar)

[[MYM]] (Maltose + Yeast Extract + Malt Extract)

Main Page

2019-05-30T11:54:12Z

Test: /* Site currently under construction! */

<h2>ActinoBase: a Streptomyces Community Wiki</h2>

===Site currently under construction!===
----
ActinoBase is a community resource, available to anyone interested studying Actinobacteria! This wiki is being created by the community and for the community to share knowledge and resources pertaining to the study of Actinomycetes such as Streptomyces.

When complete ActinoBase will provide media recipes, experimental protocols, plasmid maps and links to other useful resources. This database will be curated by the Streptomyces scientific community to ensure this resource is accurate and up to date. We hope ActinoBase will not only mediate the sharing of scientific resources amongst the existing Streptomyces community, but make accessible invaluable resources for those looking to begin studying the biology of Actinobacteria. Streptomyces bacteria have the potential to contribute solutions to global issues such as antibiotic resistance & food security through their prolific production of antibiotics and relationship with plant roots. This makes Streptomyces biology an important system to study as we seek to address these issues.

Over the coming months we will be redesigning the site layout and adding useful content, please follow us on twitter [https://twitter.com/ActinoBase @ActinoBase] or join the discussion via [https://twitter.com/hashtag/ActinoBase?src=hash #ActinoBase] to keep up to date with the our progresses! If you have any questions or requests you can email the ActinoBase committee overseeing the construction of this website at teamwikimyces@gmail.com

Updated- 30.05.2019
----

 Useful external links.

* [https://twitter.com/ActinoBase Twitter @ActinoBase]
* [http://streptomyces.org.uk Additional Streptomyces resources including the StrepDB genome database]

Recipes for buffers and growth media:

[[Media recipes]]

Protocols for purifying nucleic acids:

[[RNA preps]]

[[DNA preps]]

Main Page

2019-05-30T11:52:38Z

Test: /* Site currently under construction! */

<h2>ActinoBase: a Streptomyces Community Wiki</h2>

===Site currently under construction!===
----
ActinoBase is a community resource, available to anyone interested studying Actinobacteria! This wiki is being created by the community and for the community to share knowledge and resources pertaining to the study of Actinomycetes such as Streptomyces.

When complete ActinoBase will provide media recipes, experimental protocols, plasmid maps and links to other useful resources. This database will be curated by the Streptomyces scientific community to ensure this resource is accurate and up to date. We hope ActinoBase will not only mediate the sharing of scientific resources amongst the existing Streptomyces community, but make accessible invaluable resources for those looking to begin studying the biology of Actinobacteria. Streptomyces have the potential to contribute solutions to global issues such as antibiotic resistance & food security, making them important for study as we seek to address these issues.

Over the coming months we will be redesigning the site layout and adding useful content, please follow us on twitter [https://twitter.com/ActinoBase @ActinoBase] or join the discussion via [https://twitter.com/hashtag/ActinoBase?src=hash #ActinoBase] to keep up to date with the our progresses! If you have any questions or requests you can email the ActinoBase committee overseeing the construction of this website at teamwikimyces@gmail.com

Updated- 30.05.2019
----

 Useful external links.

* [https://twitter.com/ActinoBase Twitter @ActinoBase]
* [http://streptomyces.org.uk Additional Streptomyces resources including the StrepDB genome database]

Recipes for buffers and growth media:

[[Media recipes]]

Protocols for purifying nucleic acids:

[[RNA preps]]

[[DNA preps]]

MediaWiki:Common.css

2019-05-24T10:29:40Z

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/* CSS placed here will be applied to all skins */

* {font family: arial}

MediaWiki:Common.css

2019-05-24T10:28:20Z

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/* CSS placed here will be applied to all skins */

* {font family: courier new}

MediaWiki:Common.css

2019-05-24T10:27:29Z

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/* CSS placed here will be applied to all skins */

* {font family: arial}

Main Page

2019-05-23T15:17:36Z

Test:

<h2>ActinoBase: a Streptomyces Community Wiki</h2>

 Useful external links.

* [https://twitter.com/ActinoBase Twitter @ActinoBase]
* [http://streptomyces.org.uk Additional Streptomyces resources including the StrepDB genome database]

Recipes for buffers and growth media:

[[Media recipes]]

Protocols for purifying nucleic acids:

[[RNA preps]]

[[DNA preps]]

Main Page

2019-05-23T15:17:19Z

Test:

<h2>ActinoBase: a Streptomyces Community Wiki</h2>

 Useful external links</strong.

* [https://twitter.com/ActinoBase Twitter @ActinoBase]
* [http://streptomyces.org.uk Additional Streptomyces resources including the StrepDB genome database]

Recipes for buffers and growth media:

[[Media recipes]]

Protocols for purifying nucleic acids:

[[RNA preps]]

[[DNA preps]]

Main Page

2019-05-23T15:16:28Z

Test:

<h2>ActinoBase: a Streptomyces Community Wiki</h2>

* [https://twitter.com/ActinoBase Twitter @ActinoBase]
* [http://streptomyces.org.uk Additional Streptomyces resources including the StrepDB genome database]

Recipes for buffers and growth media:

[[Media recipes]]

Protocols for purifying nucleic acids:

[[RNA preps]]

[[DNA preps]]

Main Page

2019-05-23T14:37:06Z

Test:

ActinoBase: a Streptomyces Community Wiki

* [https://twitter.com/ActinoBase Twitter @ActinoBase]
* [http://streptomyces.org.uk Additional Streptomyces resources including the StrepDB genome database]

Recipes for buffers and growth media:

[[Media recipes]]

Protocols for purifying nucleic acids:

[[RNA preps]]

[[DNA preps]]

Main Page

2019-05-23T14:36:13Z

Test:

ActinoBase: a Streptomyces Community Wiki

* [https://twitter.com/ActinoBase Twitter @ActinoBase]
* [http://streptomyces.org.uk Additional Streptomyces resources including the StrepDB genome database]

Recipes for buffers and growth media:

[[Media recipes]]

[[Protocols for isolating RNA]]

[[Protocols for isolating DNA]]

Main Page

2019-05-23T14:35:08Z

Test:

ActinoBase: a Streptomyces Community Wiki

* [https://twitter.com/ActinoBase Twitter @ActinoBase]
* [http://streptomyces.org.uk Additional Streptomyces resources including the StrepDB genome database]

[[Media recipes]]

[[Protocols for isolating RNA]]

[[Protocols for isolating DNA]]

Main Page

2019-05-23T14:34:29Z

Test:

ActinoBase: a Streptomyces Community Wiki

* [https://twitter.com/ActinoBase Twitter @ActinoBase]
* [http://streptomyces.org.uk Additional Streptomyces resources including the StrepDB genome database]

[[Media recipes]]

[[RNA protocols]]

[[DNA preparations]]

Media recipes

2019-05-23T14:33:37Z

Test:

[[SFM]] (Soya Flour + Mannitol agar)

[[MYM]]

Media recipes

2019-05-23T14:33:10Z

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[[SFM]] Soya FLour + Mannitol agar

[[MYM]]

[[MM]]

Media recipes

2019-05-23T14:32:49Z

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[[SFM]]

[[MYM]]

[[MM]]

Media recipes

2019-05-23T14:32:32Z

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[[SFM]]

[[MYM]]

[[Minimal Medium]]

Media recipes

2019-05-23T14:32:06Z

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[[Soya Flour Mannitol Agar]]

[[MYM]]

[[Minimal Medium]]

Media recipes

2019-05-23T14:31:52Z

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[[Soya Flour Mannitol Agar]]
[[MYM]]
[[Minimal Medium]]

Media recipes

2019-05-23T14:29:35Z

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[[Soya Flour Manniol Agar]]

[[actinobase.org]]

Media recipes

2019-05-23T14:29:02Z

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[[Soya Flour Manniol Agar]]

[[home]]

Media recipes

2019-05-23T14:28:43Z

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[[Soya Flour Manniol Agar]]

Article

2019-05-23T14:27:08Z

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Media recipes

Main Page

2019-05-23T14:24:00Z

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Main Page

2019-05-23T12:43:30Z

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Streptomyces venezuelae

2019-05-06T16:39:35Z

Test: /* Sporulation */

==Organism Page==

=== ''Streptomyces venezuelae'' ===

===Background===

===Growth & Maintenance===
=====Sporulation=====
*Sporulation can be achieved after 3-5 days growth on [[MYM]] medium (liquid or solid) containing [[trace elements]].
*To make stocks, follow [[Spore Stocking]] protocol, using [[MYM]] medium.

===Links===
=====Genomes=====
=====Transcriptomes=====
=====Proteomics=====

=====Protocols=====

Streptomyces venezuelae

2019-05-06T16:36:01Z

Test: /* Proteome */

==Organism Page==

=== ''Streptomyces venezuelae'' ===

===Background===

===Growth & Maintenance===
=====Sporulation=====
*Sporulation can be achieved after 3-5 days growth on [[MYM]] medium (liquid or solid) containing [[trace elements]].
*To make stocks, follow [[Spore Stocking]] protocolm, using [[MYM]] medium.

===Links===
=====Genomes=====
=====Transcriptomes=====
=====Proteomics=====

=====Protocols=====

Streptomyces venezuelae

2019-05-06T16:35:52Z

Test: /* ==Protocols */

==Organism Page==

=== ''Streptomyces venezuelae'' ===

===Background===

===Growth & Maintenance===
=====Sporulation=====
*Sporulation can be achieved after 3-5 days growth on [[MYM]] medium (liquid or solid) containing [[trace elements]].
*To make stocks, follow [[Spore Stocking]] protocolm, using [[MYM]] medium.

===Links===
=====Genomes=====
=====Transcriptomes=====
=====Proteome=====
=====Protocols=====

Streptomyces venezuelae

2019-05-06T16:35:39Z

Test: /* Links */

==Organism Page==

=== ''Streptomyces venezuelae'' ===

===Background===

===Growth & Maintenance===
=====Sporulation=====
*Sporulation can be achieved after 3-5 days growth on [[MYM]] medium (liquid or solid) containing [[trace elements]].
*To make stocks, follow [[Spore Stocking]] protocolm, using [[MYM]] medium.

===Links===
=====Genomes=====
=====Transcriptomes=====
=====Proteome=====
=====Protocols===

Streptomyces venezuelae

2019-05-06T16:35:10Z

Test: /* Sporulation */

==Organism Page==

=== ''Streptomyces venezuelae'' ===

===Background===

===Growth & Maintenance===
=====Sporulation=====
*Sporulation can be achieved after 3-5 days growth on [[MYM]] medium (liquid or solid) containing [[trace elements]].
*To make stocks, follow [[Spore Stocking]] protocolm, using [[MYM]] medium.

===Links===
====Genomes====
====Transcriptomes====
====Proteome====
====Protocols====

Streptomyces venezuelae

2019-05-06T16:34:41Z

Test:

==Organism Page==

=== ''Streptomyces venezuelae'' ===

===Background===

===Growth & Maintenance===
=====Sporulation=====
*Sporulation can be achieved after 3-5 days growth on [[MYM medium]] (liquid or solid) containing [[trace elements]].
*To make stocks, follow [[Spore Stocking]] protocolm, using [[MYM medium]].

===Links===
====Genomes====
====Transcriptomes====
====Proteome====
====Protocols====

Streptomyces venezuelae

2019-05-06T16:28:28Z

Test:

==Organism Page==

=== ''Streptomyces venezuelae'' ===

===Background===

===Links===
====Genomes====
====Transcriptomes====
====Proteome====
====Protocols====

Streptomyces venezuelae

2019-05-06T16:25:29Z

Test: Created page with "==Organism Page== === ''Streptomyces venezuelae'' === Information not added"

==Organism Page==

=== ''Streptomyces venezuelae'' ===

Information not added

MYM

2019-05-06T16:24:35Z

Test:

=MYM Medium=

==For 1 Litre:==

*4g Maltose

*4g Yeast Extract Oxoid

*10g Malt Extract

*500ml Tap Water

*500ml dH2O

'''Instructions'''

#Dissolve all ingredients in 500ml tap water & 250ml dH2O
#pH to 7.2-7.3 with NaOH
#add dH2O up to 1L
#For solid medium add add 2g agar & 100mL liquid MYM to each flask
#Autoclave, expect a precipitate to form afterward.
#To encourage ''[[Streptomyces venezuelae]]'' sporulation, add [[trace elements]] at a 1:500 dilution.

Note: If using for growth measurements in liquid medium, it may be best to allow the precipitate to settle overnight before pipetting medium into growth vessels, taking from the top to avoid the precipitate.