{"batchcomplete":"","continue":{"lecontinue":"20250113104434|457","continue":"-||"},"query":{"logevents":[{"logid":467,"ns":2,"title":"User:AndyT","pageid":0,"logpage":0,"params":{"userid":72},"type":"newusers","action":"create2","user":"Matt Hutchings","timestamp":"2026-10-20T16:05:35Z","comment":""},{"logid":466,"ns":6,"title":"File:PSS150.png","pageid":281,"logpage":281,"params":{},"type":"create","action":"create","user":"Matt Hutchings","timestamp":"2026-03-02T11:00:25Z","comment":""},{"logid":465,"ns":6,"title":"File:PSS150.png","pageid":281,"logpage":281,"params":{"img_sha1":"n9aapj0u4s9g5zxj2lu5msyfiuw7asc","img_timestamp":"2026-03-02T11:00:25Z"},"type":"upload","action":"upload","user":"Matt Hutchings","timestamp":"2026-03-02T11:00:25Z","comment":""},{"logid":464,"ns":0,"title":"PSS150","pageid":280,"logpage":280,"params":{},"type":"create","action":"create","user":"Matt Hutchings","timestamp":"2026-03-02T10:45:01Z","comment":"Created page with \"pSS150:  pIJ10257 carrying PermE*-sfgfp, HygR  Codon-optimised sfgfp was inserted between the NdeI/XhoI of pIJ10257   Reference: Casu B, Sallmen JW, Schlimpert S, Pilhofer M. Cytoplasmic contractile injection systems mediate cell death in Streptomyces. Nat Microbiol. 2023 Apr;8(4):711-726. doi: 10.1038/s41564-023-01341-x. Epub 2023 Mar 9. PMID: 36894633; PMCID: PMC10066040.\""},{"logid":463,"ns":0,"title":"ABB13","pageid":279,"logpage":279,"params":{},"type":"create","action":"create","user":"Matt Hutchings","timestamp":"2026-01-27T12:39:13Z","comment":"Created page with \"ABB13 is a great medium for inducing antibiotic production and has also been used to improve transformation efficiency in actinomycetes.   ABB1 base medium contained the following components (g/L):  10g Bacto Soytone, 9g yeast extract, 10g soluble starch,  15g cerelose Filter sterilised 3-N-morpholinopropanesulfonic acid (MOPS) buffer, pH 7.0, was added to ABB1 base medium after autoclaving to a final concentration of 50 mM.  <strong>References</strong>  Fitzgerals et al...\""},{"logid":462,"ns":2,"title":"User:KNoble","pageid":0,"logpage":0,"params":{"userid":71},"type":"newusers","action":"create2","user":"Matt Hutchings","timestamp":"2025-11-14T15:48:46Z","comment":"Editor"},{"logid":461,"ns":0,"title":"YMS","pageid":278,"logpage":278,"params":{},"type":"create","action":"create","user":"Matt Hutchings","timestamp":"2025-11-04T12:01:06Z","comment":"Created page with \"Yeast extract: 4.0 g Malt extract: 10.0 g Soluble starch: 10.0 g (or sometimes as high as 30.0 g) Agar: 20.0 g (for solid medium) Calcium carbonate: 2.0 g (often added after adjusting pH) Distilled water: 1000 mL\u00a0\""},{"logid":460,"ns":0,"title":"ACT assay","pageid":277,"logpage":277,"params":{},"type":"create","action":"create","user":"MunnJ90","timestamp":"2025-09-04T15:42:05Z","comment":"Created page with \"=== Example Assay Description === Equal volumes of culture supernatant and 1 M NaOH were mixed by vortexing.   The samples were centrifuged at 1000 \u00d7 g for 5 min, and the absorbance at 633 nm was measured.    The molar extinction coefficient for actinorhodin is:   :<math>\\varepsilon = 15{,}135 \\ \\mathrm{M^{-1} \\, cm^{-1}}</math>    Using the Beer\u2013Lambert law (<math>A = \\varepsilon \\, l \\, c</math>), the concentration was calculated.   The specific production yield was...\""},{"logid":459,"ns":0,"title":"Streptomyces DNA isolation","pageid":276,"logpage":276,"params":{},"type":"create","action":"create","user":"MunnJ90","timestamp":"2025-09-04T15:30:18Z","comment":"Created page with \"== Streptomyces DNA Isolation == ''From 5 mL of culture''\""},{"logid":458,"ns":2,"title":"User:Sam Prudence","pageid":0,"logpage":0,"params":{"duration":"infinity","flags":["nocreate"],"sitewide":""},"type":"block","action":"block","user":"Matt Hutchings","timestamp":"2025-01-13T10:44:48Z","comment":""}]}}