MediaWiki API result
This is the HTML representation of the JSON format. HTML is good for debugging, but is unsuitable for application use.
Specify the format parameter to change the output format. To see the non-HTML representation of the JSON format, set format=json.
See the complete documentation, or the API help for more information.
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"user": "Matt Hutchings",
"timestamp": "2026-03-02T10:45:01Z",
"comment": "Created page with \"pSS150: pIJ10257 carrying PermE*-sfgfp, HygR Codon-optimised sfgfp was inserted between the NdeI/XhoI of pIJ10257 Reference: Casu B, Sallmen JW, Schlimpert S, Pilhofer M. Cytoplasmic contractile injection systems mediate cell death in Streptomyces. Nat Microbiol. 2023 Apr;8(4):711-726. doi: 10.1038/s41564-023-01341-x. Epub 2023 Mar 9. PMID: 36894633; PMCID: PMC10066040.\""
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"user": "Matt Hutchings",
"timestamp": "2026-01-27T12:39:13Z",
"comment": "Created page with \"ABB13 is a great medium for inducing antibiotic production and has also been used to improve transformation efficiency in actinomycetes. ABB1 base medium contained the following components (g/L): 10g Bacto Soytone, 9g yeast extract, 10g soluble starch, 15g cerelose Filter sterilised 3-N-morpholinopropanesulfonic acid (MOPS) buffer, pH 7.0, was added to ABB1 base medium after autoclaving to a final concentration of 50 mM. <strong>References</strong> Fitzgerals et al...\""
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"user": "Matt Hutchings",
"timestamp": "2025-11-04T12:01:06Z",
"comment": "Created page with \"Yeast extract: 4.0 g Malt extract: 10.0 g Soluble starch: 10.0 g (or sometimes as high as 30.0 g) Agar: 20.0 g (for solid medium) Calcium carbonate: 2.0 g (often added after adjusting pH) Distilled water: 1000 mL\u00a0\""
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"comment": "Created page with \"=== Example Assay Description === Equal volumes of culture supernatant and 1 M NaOH were mixed by vortexing. The samples were centrifuged at 1000 \u00d7 g for 5 min, and the absorbance at 633 nm was measured. The molar extinction coefficient for actinorhodin is: :<math>\\varepsilon = 15{,}135 \\ \\mathrm{M^{-1} \\, cm^{-1}}</math> Using the Beer\u2013Lambert law (<math>A = \\varepsilon \\, l \\, c</math>), the concentration was calculated. The specific production yield was...\""
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"timestamp": "2025-09-04T15:30:18Z",
"comment": "Created page with \"== Streptomyces DNA Isolation == ''From 5 mL of culture''\""
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