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	<id>https://actinobase.org/index.php?action=history&amp;feed=atom&amp;title=1%2F2_MS%2BB5</id>
	<title>1/2 MS+B5 - Revision history</title>
	<link rel="self" type="application/atom+xml" href="https://actinobase.org/index.php?action=history&amp;feed=atom&amp;title=1%2F2_MS%2BB5"/>
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	<updated>2026-10-17T20:23:57Z</updated>
	<subtitle>Revision history for this page on the wiki</subtitle>
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	<entry>
		<id>https://actinobase.org/index.php?title=1/2_MS%2BB5&amp;diff=1974&amp;oldid=prev</id>
		<title>Damien Gayrard at 14:18, 2 November 2021</title>
		<link rel="alternate" type="text/html" href="https://actinobase.org/index.php?title=1/2_MS%2BB5&amp;diff=1974&amp;oldid=prev"/>
		<updated>2021-11-02T14:18:58Z</updated>

		<summary type="html">&lt;p&gt;&lt;/p&gt;
&lt;table style=&quot;background-color: #fff; color: #202122;&quot; data-mw=&quot;interface&quot;&gt;
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				&lt;td colspan=&quot;2&quot; style=&quot;background-color: #fff; color: #202122; text-align: center;&quot;&gt;← Older revision&lt;/td&gt;
				&lt;td colspan=&quot;2&quot; style=&quot;background-color: #fff; color: #202122; text-align: center;&quot;&gt;Revision as of 14:18, 2 November 2021&lt;/td&gt;
				&lt;/tr&gt;&lt;tr&gt;&lt;td colspan=&quot;2&quot; class=&quot;diff-lineno&quot; id=&quot;mw-diff-left-l15&quot;&gt;Line 15:&lt;/td&gt;
&lt;td colspan=&quot;2&quot; class=&quot;diff-lineno&quot;&gt;Line 15:&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td class=&quot;diff-marker&quot;&gt;&lt;/td&gt;&lt;td style=&quot;background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;&quot;&gt;&lt;br/&gt;&lt;/td&gt;&lt;td class=&quot;diff-marker&quot;&gt;&lt;/td&gt;&lt;td style=&quot;background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;&quot;&gt;&lt;br/&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td class=&quot;diff-marker&quot;&gt;&lt;/td&gt;&lt;td style=&quot;background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;&quot;&gt;&lt;div&gt;For solid media:&lt;/div&gt;&lt;/td&gt;&lt;td class=&quot;diff-marker&quot;&gt;&lt;/td&gt;&lt;td style=&quot;background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;&quot;&gt;&lt;div&gt;For solid media:&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td class=&quot;diff-marker&quot; data-marker=&quot;−&quot;&gt;&lt;/td&gt;&lt;td style=&quot;color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;&quot;&gt;&lt;div&gt;&lt;del style=&quot;font-weight: bold; text-decoration: none;&quot;&gt;#&lt;/del&gt;Add 12 g Bacto Agar&lt;/div&gt;&lt;/td&gt;&lt;td class=&quot;diff-marker&quot; data-marker=&quot;+&quot;&gt;&lt;/td&gt;&lt;td style=&quot;color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;&quot;&gt;&lt;div&gt;Add 12 g Bacto Agar&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td class=&quot;diff-marker&quot;&gt;&lt;/td&gt;&lt;td style=&quot;background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;&quot;&gt;&lt;br/&gt;&lt;/td&gt;&lt;td class=&quot;diff-marker&quot;&gt;&lt;/td&gt;&lt;td style=&quot;background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;&quot;&gt;&lt;br/&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td class=&quot;diff-marker&quot;&gt;&lt;/td&gt;&lt;td style=&quot;background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;&quot;&gt;&lt;div&gt;==Uses==&lt;/div&gt;&lt;/td&gt;&lt;td class=&quot;diff-marker&quot;&gt;&lt;/td&gt;&lt;td style=&quot;background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;&quot;&gt;&lt;div&gt;==Uses==&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td class=&quot;diff-marker&quot;&gt;&lt;/td&gt;&lt;td style=&quot;background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;&quot;&gt;&lt;div&gt;*[[Growing Marchantia from gemma spores]]&lt;/div&gt;&lt;/td&gt;&lt;td class=&quot;diff-marker&quot;&gt;&lt;/td&gt;&lt;td style=&quot;background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;&quot;&gt;&lt;div&gt;*[[Growing Marchantia from gemma spores]]&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;/table&gt;</summary>
		<author><name>Damien Gayrard</name></author>
	</entry>
	<entry>
		<id>https://actinobase.org/index.php?title=1/2_MS%2BB5&amp;diff=1973&amp;oldid=prev</id>
		<title>Damien Gayrard: Created page with &quot;==Preparation== Per Litre: *2.205 MS Salts and Gamborg B5 Vitamins (Duchefa M0231) *1 L ddH2O *5M KOH *Bacto Agar   &#039;&#039;&#039;Instructions&#039;&#039;&#039; #Dissolve 2.2g MS Salts + Gamborg B5 Vit...&quot;</title>
		<link rel="alternate" type="text/html" href="https://actinobase.org/index.php?title=1/2_MS%2BB5&amp;diff=1973&amp;oldid=prev"/>
		<updated>2021-11-02T14:18:40Z</updated>

		<summary type="html">&lt;p&gt;Created page with &amp;quot;==Preparation== Per Litre: *2.205 MS Salts and Gamborg B5 Vitamins (Duchefa M0231) *1 L ddH2O *5M KOH *Bacto Agar   &amp;#039;&amp;#039;&amp;#039;Instructions&amp;#039;&amp;#039;&amp;#039; #Dissolve 2.2g MS Salts + Gamborg B5 Vit...&amp;quot;&lt;/p&gt;
&lt;p&gt;&lt;b&gt;New page&lt;/b&gt;&lt;/p&gt;&lt;div&gt;==Preparation==&lt;br /&gt;
Per Litre:&lt;br /&gt;
*2.205 MS Salts and Gamborg B5 Vitamins (Duchefa M0231)&lt;br /&gt;
*1 L ddH2O&lt;br /&gt;
*5M KOH&lt;br /&gt;
*Bacto Agar&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Instructions&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
#Dissolve 2.2g MS Salts + Gamborg B5 Vitamin mix in 950ml ddH2O&lt;br /&gt;
#Adjust pH to 6.7 with 5M KOH &lt;br /&gt;
#Make up to final volume with water (for 1L, add the remaining ≈50ml ddH2O)&lt;br /&gt;
#Divide into batches (in bottles containing Bacto Agar if required)&lt;br /&gt;
#Autoclave until golden brown&lt;br /&gt;
&lt;br /&gt;
For solid media:&lt;br /&gt;
#Add 12 g Bacto Agar&lt;br /&gt;
&lt;br /&gt;
==Uses==&lt;br /&gt;
*[[Growing Marchantia from gemma spores]]&lt;/div&gt;</summary>
		<author><name>Damien Gayrard</name></author>
	</entry>
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