ABB13 - Revision history

Matt Hutchings at 12:44, 27 January 2026

2026-01-27T12:44:32Z

← Older revision		Revision as of 12:44, 27 January 2026
Line 6:		Line 6:
	*10g soluble starch,		*10g soluble starch,
	*15g cerelose		*15g cerelose
	*~~Filter sterilised 3-N-morpholinopropanesulfonic acid (MOPS) buffer,~~ pH 7.0~~, was added~~ to o a final concentration of 50 mM after autoclaving.		*Add pH 7.0 filter sterilised MOPS buffer to a final concentration of 50 mM after autoclaving.

	<strong>References</strong>		<strong>References</strong>

	Fitzgerald <em>et al</em> (1998). Sonication-Dependent Electroporation of the Erythromycin-Producing Bacterium <em>Saccharopolyspora erythraea</em>. AEM 674:1580–1583. https://journals.asm.org/doi/10.1128/aem.64.4.1580-1583.1998		Fitzgerald <em>et al</em> (1998). Sonication-Dependent Electroporation of the Erythromycin-Producing Bacterium <em>Saccharopolyspora erythraea</em>. AEM 674:1580–1583. https://journals.asm.org/doi/10.1128/aem.64.4.1580-1583.1998

Matt Hutchings at 12:41, 27 January 2026

2026-01-27T12:41:51Z

← Older revision		Revision as of 12:41, 27 January 2026
Line 6:		Line 6:
	*10g soluble starch,		*10g soluble starch,
	*15g cerelose		*15g cerelose
	*Filter sterilised 3-N-morpholinopropanesulfonic acid (MOPS) buffer, pH 7.0, was added to ~~ABB1 base medium after autoclaving to~~ a final concentration of 50 mM.		*Filter sterilised 3-N-morpholinopropanesulfonic acid (MOPS) buffer, pH 7.0, was added to o a final concentration of 50 mM after autoclaving.

	<strong>References</strong>		<strong>References</strong>

	Fitzgerald <em>et al</em> (1998). Sonication-Dependent Electroporation of the Erythromycin-Producing Bacterium <em>Saccharopolyspora erythraea</em>. AEM 674:1580–1583. https://journals.asm.org/doi/10.1128/aem.64.4.1580-1583.1998		Fitzgerald <em>et al</em> (1998). Sonication-Dependent Electroporation of the Erythromycin-Producing Bacterium <em>Saccharopolyspora erythraea</em>. AEM 674:1580–1583. https://journals.asm.org/doi/10.1128/aem.64.4.1580-1583.1998

Matt Hutchings at 12:41, 27 January 2026

2026-01-27T12:41:17Z

← Older revision		Revision as of 12:41, 27 January 2026
Line 10:		Line 10:
	<strong>References</strong>		<strong>References</strong>

	~~Fitzgerals~~ et al (1998). Sonication-Dependent Electroporation of the Erythromycin-Producing Bacterium Saccharopolyspora erythraea. AEM 674:1580–1583. https://journals.asm.org/doi/10.1128/aem.64.4.1580-1583.1998		Fitzgerald <em>et al</em> (1998). Sonication-Dependent Electroporation of the Erythromycin-Producing Bacterium <em>Saccharopolyspora erythraea</em>. AEM 674:1580–1583. https://journals.asm.org/doi/10.1128/aem.64.4.1580-1583.1998

Matt Hutchings at 12:40, 27 January 2026

2026-01-27T12:40:00Z

← Older revision		Revision as of 12:40, 27 January 2026
Line 2:		Line 2:

	ABB1 base medium contained the following components (g/L):		ABB1 base medium contained the following components (g/L):
	10g Bacto Soytone,		*10g Bacto Soytone,
	9g yeast extract,		*9g yeast extract,
	10g soluble starch,		*10g soluble starch,
	15g cerelose		*15g cerelose
	Filter sterilised 3-N-morpholinopropanesulfonic acid (MOPS) buffer, pH 7.0, was added to ABB1 base medium after autoclaving to a final concentration of 50 mM.		*Filter sterilised 3-N-morpholinopropanesulfonic acid (MOPS) buffer, pH 7.0, was added to ABB1 base medium after autoclaving to a final concentration of 50 mM.

	<strong>References</strong>		<strong>References</strong>

	Fitzgerals et al (1998). Sonication-Dependent Electroporation of the Erythromycin-Producing Bacterium Saccharopolyspora erythraea. AEM 674:1580–1583. https://journals.asm.org/doi/10.1128/aem.64.4.1580-1583.1998		Fitzgerals et al (1998). Sonication-Dependent Electroporation of the Erythromycin-Producing Bacterium Saccharopolyspora erythraea. AEM 674:1580–1583. https://journals.asm.org/doi/10.1128/aem.64.4.1580-1583.1998

Matt Hutchings: Created page with "ABB13 is a great medium for inducing antibiotic production and has also been used to improve transformation efficiency in actinomycetes. ABB1 base medium contained the following components (g/L): 10g Bacto Soytone, 9g yeast extract, 10g soluble starch, 15g cerelose Filter sterilised 3-N-morpholinopropanesulfonic acid (MOPS) buffer, pH 7.0, was added to ABB1 base medium after autoclaving to a final concentration of 50 mM. References Fitzgerals et al..."

2026-01-27T12:39:13Z

Created page with "ABB13 is a great medium for inducing antibiotic production and has also been used to improve transformation efficiency in actinomycetes. ABB1 base medium contained the following components (g/L): 10g Bacto Soytone, 9g yeast extract, 10g soluble starch, 15g cerelose Filter sterilised 3-N-morpholinopropanesulfonic acid (MOPS) buffer, pH 7.0, was added to ABB1 base medium after autoclaving to a final concentration of 50 mM. <strong>References</strong> Fitzgerals et al..."

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ABB13 is a great medium for inducing antibiotic production and has also been used to improve transformation efficiency in actinomycetes.

ABB1 base medium contained the following components (g/L):
10g Bacto Soytone,
9g yeast extract,
10g soluble starch,
15g cerelose
Filter sterilised 3-N-morpholinopropanesulfonic acid (MOPS) buffer, pH 7.0, was added to ABB1 base medium after autoclaving to a final concentration of 50 mM.

<strong>References</strong>

Fitzgerals et al (1998). Sonication-Dependent Electroporation of the Erythromycin-Producing Bacterium Saccharopolyspora erythraea. AEM 674:1580–1583. https://journals.asm.org/doi/10.1128/aem.64.4.1580-1583.1998