Antibiotic stocks and working concentrations - Revision history

DaveMark at 13:38, 9 August 2019

2019-08-09T13:38:42Z

← Older revision		Revision as of 13:38, 9 August 2019
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	\| style="text-align: center;" \| 50		\| style="text-align: center;" \| 50
	\|-		\|-
	\| ~~Hygromycin~~		\| Carbenicillin<sup>1</sub>
	\| style="text-align: center;" \| 50 in dH<sub>2</sub>O		\| style="text-align: center;" \| 100 in dH<sub>2</sub>O
	\| style="text-align: center;" \| 100		\| style="text-align: center;" \| 100
	\|-		\|-
	\| ~~Kanamycin~~		\| Chloramphenicol
	\| style="text-align: center;" \| 50 in ~~dH<sub>2</sub>O~~		\| style="text-align: center;" \| 25 in 100% EtOH
	\| style="text-align: center;" \| 50		\| style="text-align: center;" \| 25
	\|-		\|-
	\| ~~Chloramphenicol~~		\| Cycloheximide<sup>2</sup>
	\| style="text-align: center;" \| 25 in 100% EtOH		\| style="text-align: center;" \| 25 in 100% EtOH
	\| style="text-align: center;" \| 25		\| style="text-align: center;" \| 25
	\|-		\|-
	\| ~~Carbenicillin~~		\| Hygromycin<sup>3</sub>
	\| style="text-align: center;" \| ~~100~~ in dH<sub>2</sub>O		\| style="text-align: center;" \| 50 in dH<sub>2</sub>O
	\| style="text-align: center;" \| 100		\| style="text-align: center;" \| 100
	\|-		\|-
	\| ~~Thiostrepton~~		\| Kanamycin
	\| style="text-align: center;" \| 50 in ~~100% DMSO~~		\| style="text-align: center;" \| 50 in dH<sub>2</sub>O
	\| style="text-align: center;" \| 50		\| style="text-align: center;" \| 50
	\|-		\|-
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	\| style="text-align: center;" \| 25 in 0.2N NaOH		\| style="text-align: center;" \| 25 in 0.2N NaOH
	\| style="text-align: center;" \| 25		\| style="text-align: center;" \| 25
			\|-
			\| Thiostrepton
			\| style="text-align: center;" \| 50 in 100% DMSO
			\| style="text-align: center;" \| 50
	\|}		\|}

	<strong>Notes:</strong>		<strong>Notes:</strong>
	*Hygromycin selection is salt-sensitive; on low-salt media such as [[DNA]], 50 µg /ml (final concentration) is sufficient for selection.
	*Carbenicillin is more stable than ampicillin, but care should still be taken to avoid excessive freeze/thaw cycles which could result in degradation of the antibiotic.		<sup>1</sup>Carbenicillin is more stable than ampicillin, but care should still be taken to avoid excessive freeze/thaw cycles which could result in degradation of the antibiotic.

			<sup>2</sup>Cycloheximide is toxic, prepare the solution in a fume hood and take care when handling

			<sup>3</sup>Hygromycin selection is salt-sensitive; on low-salt media such as [[DNA]], 50 µg /ml (final concentration) is sufficient for selection.

MFeeney at 13:52, 31 July 2019

2019-07-31T13:52:10Z

← Older revision		Revision as of 13:52, 31 July 2019
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	The following antibiotics can be used for selection at these concentrations in ~~most~~ <em>Streptomyces</em> sp. (If working with a new species, you should always first check whether it has intrinsic resistance to an antibiotic before attempting to use it.)		The following antibiotics can be used for selection at these concentrations in many <em>Streptomyces</em> sp. (If working with a new species, you should always first check whether it has intrinsic resistance to an antibiotic before attempting to use it. Generally speaking, apramycin and hygromycin are likely to be more useful as selective markers than e.g. kanamycin and carbenicillin.)

	{\| class="wikitable"		{\| class="wikitable"

MFeeney at 13:50, 31 July 2019

2019-07-31T13:50:13Z

← Older revision		Revision as of 13:50, 31 July 2019
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			The following antibiotics can be used for selection at these concentrations in most <em>Streptomyces</em> sp. (If working with a new species, you should always first check whether it has intrinsic resistance to an antibiotic before attempting to use it.)

	{\| class="wikitable"		{\| class="wikitable"
	! style="font-weight:bold; background-color:#c0c0c0;" \| Antibiotic		! style="font-weight:bold; background-color:#c0c0c0;" \| Antibiotic
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	\|-		\|-
	\| Nalidixic acid		\| Nalidixic acid
	\| style="text-align: center;" \| 25 in ~~dH<sub>2</sub>O~~		\| style="text-align: center;" \| 25 in 0.2N NaOH
	\| style="text-align: center;" \| 25		\| style="text-align: center;" \| 25
	\|}		\|}

			<strong>Notes:</strong>
			*Hygromycin selection is salt-sensitive; on low-salt media such as [[DNA]], 50 µg /ml (final concentration) is sufficient for selection.
			*Carbenicillin is more stable than ampicillin, but care should still be taken to avoid excessive freeze/thaw cycles which could result in degradation of the antibiotic.

MFeeney: Created page with "{| class="wikitable" ! style="font-weight:bold; background-color:#c0c0c0;" | Antibiotic ! style="font-weight:bold; background-color:#c0c0c0;" | Stock [mg/ml] ! style="font-wei..."

2019-07-31T13:39:53Z

Created page with "{| class="wikitable" ! style="font-weight:bold; background-color:#c0c0c0;" | Antibiotic ! style="font-weight:bold; background-color:#c0c0c0;" | Stock [mg/ml] ! style="font-wei..."

New page

{| class="wikitable"
! style="font-weight:bold; background-color:#c0c0c0;" | Antibiotic
! style="font-weight:bold; background-color:#c0c0c0;" | Stock [mg/ml]
! style="font-weight:bold; background-color:#c0c0c0;" | Final concentration [µg /ml]
|-
| Apramycin
| style="text-align: center;" | 100 in dH2O
| style="text-align: center;" | 50
|-
| Hygromycin
| style="text-align: center;" | 50 in dH2O
| style="text-align: center;" | 100
|-
| Kanamycin
| style="text-align: center;" | 50 in dH2O
| style="text-align: center;" | 50
|-
| Chloramphenicol
| style="text-align: center;" | 25 in 100% EtOH
| style="text-align: center;" | 25
|-
| Carbenicillin
| style="text-align: center;" | 100 in dH2O
| style="text-align: center;" | 100
|-
| Thiostrepton
| style="text-align: center;" | 50 in 100% DMSO
| style="text-align: center;" | 50
|-
| Nalidixic acid
| style="text-align: center;" | 25 in dH2O
| style="text-align: center;" | 25
|}