CM - Revision history

Matt Hutchings: /* Preparation */

2026-03-02T10:06:28Z

Preparation

← Older revision		Revision as of 10:06, 2 March 2026
Line 13:		Line 13:
	*1mL vitamin solution		*1mL vitamin solution
	*25g glucose		*25g glucose
	*~~200g~~ agar		*20g agar
	*1L dH<sub>2</sub>O		*1L dH<sub>2</sub>O

Line 40:		Line 40:
	#Dispense 200ml into flasks each containing 40g agar		#Dispense 200ml into flasks each containing 40g agar
	#Autoclave		#Autoclave


	==Uses==		==Uses==

Matt Hutchings at 09:55, 2 March 2026

2026-03-02T09:55:20Z

← Older revision		Revision as of 09:55, 2 March 2026
Line 13:		Line 13:
	*1mL vitamin solution		*1mL vitamin solution
	*25g glucose		*25g glucose
	*~~10g~~ agar		*200g agar
	*1L dH<sub>2</sub>O		*1L dH<sub>2</sub>O

Line 38:		Line 38:
	#Adjust the pH to 7.2 with 1N HCl		#Adjust the pH to 7.2 with 1N HCl
	#Add the vitamin solution		#Add the vitamin solution
	#Dispense 200ml into flasks each containing 2g agar		#Dispense 200ml into flasks each containing 40g agar
	#Autoclave		#Autoclave

MFeeney at 11:52, 31 July 2019

2019-07-31T11:52:17Z

← Older revision		Revision as of 11:52, 31 July 2019
Line 43:		Line 43:

	==Uses==		==Uses==
	*A non-selective medium useful for genetic crosses (the progeny from such crosses can subsequently be analysed on appropriate selective media such as [[MM]]<sup>[1]</sup>		*A non-selective medium useful for genetic crosses (the progeny from such crosses can subsequently be analysed on appropriate selective media such as [[MM]])<sup>[1]</sup>

	==Notes==		==Notes==

MFeeney at 11:50, 31 July 2019

2019-07-31T11:50:58Z

← Older revision		Revision as of 11:50, 31 July 2019
Line 35:		Line 35:
	'''Instructions'''		'''Instructions'''
	#Make up the yeast nucleic acid hydrolysate according to the directions above: do not sterilize. Make up the vitamin solution following the recipe above and sterilize by autoclaving (115˚C, 10 min).		#Make up the yeast nucleic acid hydrolysate according to the directions above: do not sterilize. Make up the vitamin solution following the recipe above and sterilize by autoclaving (115˚C, 10 min).
	Dissolve all of the ingredients, except the agar and the vitamin solution, but including the glucose, in the distilled water		#Dissolve all of the ingredients, except the agar and the vitamin solution, but including the glucose, in the distilled water
	#Adjust the pH to 7.2 with 1N HCl		#Adjust the pH to 7.2 with 1N HCl
	#Add the vitamin solution		#Add the vitamin solution
Line 43:		Line 43:

	==Uses==		==Uses==
			*A non-selective medium useful for genetic crosses (the progeny from such crosses can subsequently be analysed on appropriate selective media such as [[MM]]<sup>[1]</sup>

	==Notes==		==Notes==
			<sup>*</sup>The histidine, proline, tryptophan, and tyrosine should be added for the relevant auxotrophic mutants; if not working with auxotrophs, any/all of these amino acids can be omitted.

	==References==		==References==
	Hopwood DA. Genetic analysis and genome structure in Streptomyces coelicolor. Bacteriol Rev. 1967;31(4):373–403.		[1] Hopwood DA. Genetic analysis and genome structure in Streptomyces coelicolor. Bacteriol Rev. 1967;31(4):373–403.

	Kieser, T., Bibb, M.J., Buttner, M.J., Chater, K.F. and Hopwood, D.A. (2000) Practical Streptomyces Genetics: John Innes Foundation, Norwich Research Park, Colney, Norwich NR4 7UH, UK		[2] Kieser, T., Bibb, M.J., Buttner, M.J., Chater, K.F. and Hopwood, D.A. (2000) Practical Streptomyces Genetics: John Innes Foundation, Norwich Research Park, Colney, Norwich NR4 7UH, UK

MFeeney at 11:44, 31 July 2019

2019-07-31T11:44:59Z

← Older revision		Revision as of 11:44, 31 July 2019
Line 9:		Line 9:
	*2g Difco Bacto-peptone		*2g Difco Bacto-peptone
	*1.5g Difco Casaminoacids		*1.5g Difco Casaminoacids
	50mg <strong>each</strong>L-histidine, L-proline, L-tryptophan, and L-tyrosine<sup></sup>		50mg <strong>each</strong> L-histidine, L-proline, L-tryptophan, and L-tyrosine<sup></sup>
	*5mL Yeast nucleic acid hydrolysate		*5mL Yeast nucleic acid hydrolysate
	*1mL vitamin solution		*1mL vitamin solution
Line 17:		Line 17:

	<strong>Yeast nucleic acid hydrolysate</strong>		<strong>Yeast nucleic acid hydrolysate</strong>
			#Boil 2g yeast nucleic acid in 15mL 1N HCl for 10 min.
			#Boil 2g yeast nucleic acid in 15mL 1N NaOH for 10 min.
			#Mix the two solutions and adjust to pH6
			#Filter through filter paper while hot
			#Make up the volume to 40mL with water
			#Do not sterilize

	<strong>Vitamin solution</strong>		<strong>Vitamin solution</strong>

MFeeney: Created page with "=CM (Complete Medium)= ==Preparation== Per litre: 5g K₂HPO₄ 0.5g NaCl 0.5g MgSO₄7H₂O 2g Difco Bacto-peptone 1.5g Difco Casa..."

2019-07-31T11:06:50Z

Created page with "=CM (Complete Medium)= ==Preparation== Per litre: *5g K2HPO4 *0.5g NaCl *0.5g MgSO4*7H2O *2g Difco Bacto-peptone *1.5g Difco Casa..."

New page

=CM (Complete Medium)=

==Preparation==
Per litre:
*5g K2HPO4
*0.5g NaCl
*0.5g MgSO4*7H2O
*2g Difco Bacto-peptone
*1.5g Difco Casaminoacids
*50mg eachL-histidine, L-proline, L-tryptophan, and L-tyrosine*
*5mL Yeast nucleic acid hydrolysate
*1mL vitamin solution
*25g glucose
*10g agar
*1L dH2O

Yeast nucleic acid hydrolysate

Vitamin solution
*100mg riboglavine
*100mg nicotinamide
*10mg p-aminobenzoic acid
*50mg pyridoxine HCl
*50mg thiamine HCl
*20 mg biotin
*100mL water

'''Instructions'''
#Make up the yeast nucleic acid hydrolysate according to the directions above: do not sterilize. Make up the vitamin solution following the recipe above and sterilize by autoclaving (115˚C, 10 min).
Dissolve all of the ingredients, except the agar and the vitamin solution, but including the glucose, in the distilled water
#Adjust the pH to 7.2 with 1N HCl
#Add the vitamin solution
#Dispense 200ml into flasks each containing 2g agar
#Autoclave

==Uses==

==Notes==

==References==
Hopwood DA. Genetic analysis and genome structure in Streptomyces coelicolor. Bacteriol Rev. 1967;31(4):373–403.

Kieser, T., Bibb, M.J., Buttner, M.J., Chater, K.F. and Hopwood, D.A. (2000) Practical Streptomyces Genetics: John Innes Foundation, Norwich Research Park, Colney, Norwich NR4 7UH, UK