LisAlgora: Created page with "Roche provides a DIG DNA Labelling and Detection Kit that contains: blocking reagent, Anti-DIG-AP conjugate, Hexanucleotide Mix, DIG DNA Labelling Mix, Klenow Enzyme an..."

2020-04-23T21:55:28Z

Created page with "Roche provides a <b>DIG DNA Labelling and Detection Kit</b> that contains: blocking reagent, Anti-DIG-AP conjugate, Hexanucleotide Mix, DIG DNA Labelling Mix, Klenow Enzyme an..."

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Roche provides a <b>DIG DNA Labelling and Detection Kit</b> that contains: blocking reagent, Anti-DIG-AP conjugate, Hexanucleotide Mix, DIG DNA Labelling Mix, Klenow Enzyme and NBT/BCIP solution. Otherwise, the components can be purchased separately.

{| class="wikitable"
! style="font-family:Arial, Helvetica, sans-serif !important;; background-color:rgb(222, 234, 246); color:rgb(0, 0, 0);" |
! style="font-weight:bold; font-family:Arial, Helvetica, sans-serif !important;; background-color:rgb(222, 234, 246); color:rgb(0, 0, 0);" | Item
! style="font-weight:bold; font-family:Arial, Helvetica, sans-serif !important;; background-color:rgb(222, 234, 246); color:rgb(0, 0, 0);" | Recommended Supplier
! style="font-weight:bold; font-family:Arial, Helvetica, sans-serif !important;; background-color:rgb(222, 234, 246); color:rgb(0, 0, 0);" | Usage
! style="font-weight:bold; font-family:Arial, Helvetica, sans-serif !important;; background-color:rgb(222, 234, 246); color:rgb(0, 0, 0);" | Storage
|-
| rowspan="4" style="font-weight:bold; font-family:Arial, Helvetica, sans-serif !important;; color:rgb(0, 0, 0);" | General
| style="font-family:Arial, Helvetica, sans-serif !important;; color:rgb(0, 0, 0);" | Hybond N+ membrane (rolls)
| style="font-family:Arial, Helvetica, sans-serif !important;; color:rgb(0, 0, 0);" | GE Healthcare
| style="font-family:Arial, Helvetica, sans-serif !important;; color:rgb(0, 0, 0);" | ̴ 20 blots
| style="font-family:Arial, Helvetica, sans-serif !important;; color:rgb(0, 0, 0);" | RT
|-
| style="font-family:Arial, Helvetica, sans-serif !important;; color:rgb(0, 0, 0);" | Whatman paper
| style="font-family:Arial, Helvetica, sans-serif !important;; color:rgb(0, 0, 0);" | VWR or Fisher
| style="font-family:Arial, Helvetica, sans-serif !important;; color:rgb(0, 0, 0);" | ̴ 100 blots
| style="font-family:Arial, Helvetica, sans-serif !important;; color:rgb(0, 0, 0);" | RT
|-
| style="font-family:Arial, Helvetica, sans-serif !important;; color:rgb(0, 0, 0);" | Blocking reagent
| style="font-family:Arial, Helvetica, sans-serif !important;; color:rgb(0, 0, 0);" | Roche
| style="font-family:Arial, Helvetica, sans-serif !important;; color:rgb(0, 0, 0);" | ̴ 25 blots
| style="font-family:Arial, Helvetica, sans-serif !important;; color:rgb(0, 0, 0);" | 4°C
|-
| style="font-family:Arial, Helvetica, sans-serif !important;; color:rgb(0, 0, 0);" | Anti-DIG-AP conjugate
| style="font-family:Arial, Helvetica, sans-serif !important;; color:rgb(0, 0, 0);" | Roche
| style="font-family:Arial, Helvetica, sans-serif !important;; color:rgb(0, 0, 0);" | ̴ 60 reactions
| style="font-family:Arial, Helvetica, sans-serif !important;; color:rgb(0, 0, 0);" | -20°C
|-
| rowspan="2" style="font-weight:bold; font-family:Arial, Helvetica, sans-serif !important;; color:rgb(0, 0, 0);" | To label probe
| style="font-family:Arial, Helvetica, sans-serif !important;; color:rgb(0, 0, 0);" | Either: Hexanucleotide Mix + DIG DNA Labelling Mix +Klenow Enzyme
| style="font-family:Arial, Helvetica, sans-serif !important;; color:rgb(0, 0, 0);" | Roche
| style="font-family:Arial, Helvetica, sans-serif !important;; color:rgb(0, 0, 0);" | 25 to 50 reactions
| style="font-family:Arial, Helvetica, sans-serif !important;; color:rgb(0, 0, 0);" | -20°C
|-
| style="font-family:Arial, Helvetica, sans-serif !important;; color:rgb(0, 0, 0);" | Or: PCR DIG labelling mix + PCR components
| style="font-family:Arial, Helvetica, sans-serif !important;; color:rgb(0, 0, 0);" | Roche
| style="font-family:Arial, Helvetica, sans-serif !important;; color:rgb(0, 0, 0);" | 100 reactions
| style="font-family:Arial, Helvetica, sans-serif !important;; color:rgb(0, 0, 0);" | -20°C
|-
| rowspan="2" style="font-weight:bold; color:rgb(0, 0, 0);" | For detection
| style="color:rgb(0, 0, 0);" | Either: NBT/BCIP tablets
| style="color:rgb(0, 0, 0);" | Roche
| style="font-family:Arial, Helvetica, sans-serif !important;; color:rgb(0, 0, 0);" | 10-20 blots
| style="font-family:Arial, Helvetica, sans-serif !important;; color:rgb(0, 0, 0);" | 4°C
|-
| style="font-family:Arial, Helvetica, sans-serif !important;; color:rgb(0, 0, 0);" | Or: NBT/BCIP solution
| style="font-family:Arial, Helvetica, sans-serif !important;; color:rgb(0, 0, 0);" | Roche
| style="font-family:Arial, Helvetica, sans-serif !important;; color:rgb(0, 0, 0);" |
| style="font-family:Arial, Helvetica, sans-serif !important;; color:rgb(0, 0, 0);" | -20°C
|}

Also required:
**Ammonium acetate
**EDTA
**HCl concetrated
**Maleic acid
**N-laurylsarcosine
**NaCl
**Sodium Citrate
**SDS
**NaOH
**Tris Base
**Tween-20

Consumables for Southern Blotting - Revision history

LisAlgora: Created page with "Roche provides a DIG DNA Labelling and Detection Kit that contains: blocking reagent, Anti-DIG-AP conjugate, Hexanucleotide Mix, DIG DNA Labelling Mix, Klenow Enzyme an..."