DNA - Revision history

Matt Hutchings: /* Preparation */

2026-03-02T10:06:47Z

Preparation

← Older revision		Revision as of 10:06, 2 March 2026
Line 8:		Line 8:
	Per Litre:		Per Litre:
	*4g Difco Nutrient Broth Powder		*4g Difco Nutrient Broth Powder
	*~~200g~~ Agar		*20g Agar
	*1L dH<sub>2</sub>O		*1L dH<sub>2</sub>O

	'''Instructions'''		'''Instructions'''
	#For solid medium, add 0.8g of DNB powder into each 250ml conical flask		#For solid medium, add 0.8g of DNB powder into each 250ml conical flask
	#Add ~~40g~~ of Agar		#Add 4g of Agar
	#Add 200ml dH<sub>2</sub>O		#Add 200ml dH<sub>2</sub>O
	#Autoclave		#Autoclave

Matt Hutchings: /* Preparation */

2026-03-02T10:04:36Z

Preparation

← Older revision		Revision as of 10:04, 2 March 2026
Line 8:		Line 8:
	Per Litre:		Per Litre:
	*4g Difco Nutrient Broth Powder		*4g Difco Nutrient Broth Powder
	*~~10g~~ Agar		*200g Agar
	*1L dH<sub>2</sub>O		*1L dH<sub>2</sub>O

	'''Instructions'''		'''Instructions'''
	#For solid medium, add 0.8g of DNB powder into each 250ml conical flask		#For solid medium, add 0.8g of DNB powder into each 250ml conical flask
	#Add 2g of Agar		#Add 40g of Agar
	#Add 200ml dH<sub>2</sub>O		#Add 200ml dH<sub>2</sub>O
	#Autoclave		#Autoclave


	==Uses==		==Uses==

MFeeney at 12:50, 5 May 2020

2020-05-05T12:50:24Z

← Older revision		Revision as of 12:50, 5 May 2020
Line 23:		Line 23:
	*[[Re-isolation experiments]]		*[[Re-isolation experiments]]
	*[[Selection for resistance to salt-sensitive antibiotics]]		*[[Selection for resistance to salt-sensitive antibiotics]]
			*[[Streptomyces Bacteriophage plaque assay]]


	==Notes==		==Notes==

Sam Prudence: /* Notes */

2019-11-07T16:14:26Z

Notes

← Older revision		Revision as of 16:14, 7 November 2019
Line 26:		Line 26:
	==Notes==		==Notes==
	For growth in liquid culture, [[DNB]] can be made without the agar.		For growth in liquid culture, [[DNB]] can be made without the agar.

			According to the recipe in Practical Streptomyces Genetics, it should be 8g of DNB per litre, however some groups choose to use 4g instead. This has little effect on the ability of ''Streptomyces'' to grow on the soild medium, [[DNA]].

	==References==		==References==

LisAlgora at 14:37, 26 July 2019

2019-07-26T14:37:14Z

← Older revision		Revision as of 14:37, 26 July 2019
Line 3:		Line 3:
	Difco Nutrient Agar is a rich medium commercially available from BD and routinely used for cultivating many non-fastidious organisms. It contains beef extract and peptones; exact composition will vary between batches.		Difco Nutrient Agar is a rich medium commercially available from BD and routinely used for cultivating many non-fastidious organisms. It contains beef extract and peptones; exact composition will vary between batches.

	Many Streptomyces sp. grow well on DNA, but developmental and/or antibiotic production phenotypes may differ compared to growth on other media (e.g. [[SFM]]).		Many ''Streptomyces'' sp. grow well on DNA, but developmental and/or antibiotic production phenotypes may differ compared to growth on other media (e.g. [[SFM]]).

	==Preparation==		==Preparation==

Test at 15:59, 4 July 2019

2019-07-04T15:59:23Z

← Older revision		Revision as of 15:59, 4 July 2019
Line 19:		Line 19:

	==Uses==		==Uses==
	*[[~~Streptomyces Colony~~ PCR]]		*''Streptomyces'' Colony PCR (see [[High GC PCR]] for instructions)
	*[[Spore titres]]		*[[Spore titres]]
	*[[Re-isolation experiments]]		*[[Re-isolation experiments]]

MFeeney at 12:57, 2 July 2019

2019-07-02T12:57:26Z

← Older revision		Revision as of 12:57, 2 July 2019
Line 9:		Line 9:
	*4g Difco Nutrient Broth Powder		*4g Difco Nutrient Broth Powder
	*10g Agar		*10g Agar
	*1L ~~dH2O~~		*1L dH<sub>2</sub>O

	'''Instructions'''		'''Instructions'''
	#For solid medium, add 0.8g of DNB powder into each 250ml conical flask		#For solid medium, add 0.8g of DNB powder into each 250ml conical flask
	#Add 2g of Agar		#Add 2g of Agar
	#Add 200ml ~~dH2O~~		#Add 200ml dH<sub>2</sub>O
	#Autoclave		#Autoclave

ActinoCommittee at 14:58, 25 June 2019

2019-06-25T14:58:51Z

← Older revision		Revision as of 14:58, 25 June 2019
Line 25:		Line 25:

	==Notes==		==Notes==
	For growth in liquid culture, [[~~Difco Nutrient Broth~~]] can be made without the agar.		For growth in liquid culture, [[DNB]] can be made without the agar.

	==References==		==References==

ActinoCommittee: Created page with "=Difco Nutrient Agar (DNA)= Difco Nutrient Agar is a rich medium commercially available from BD and routinely used for cultivating many non-fastidious organisms. It contains..."

2019-06-25T14:58:31Z

Created page with "=Difco Nutrient Agar (DNA)= Difco Nutrient Agar is a rich medium commercially available from BD and routinely used for cultivating many non-fastidious organisms. It contains..."

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=Difco Nutrient Agar (DNA)=

Difco Nutrient Agar is a rich medium commercially available from BD and routinely used for cultivating many non-fastidious organisms. It contains beef extract and peptones; exact composition will vary between batches.

Many Streptomyces sp. grow well on DNA, but developmental and/or antibiotic production phenotypes may differ compared to growth on other media (e.g. [[SFM]]).

==Preparation==
Per Litre:
*4g Difco Nutrient Broth Powder
*10g Agar
*1L dH2O

'''Instructions'''
#For solid medium, add 0.8g of DNB powder into each 250ml conical flask
#Add 2g of Agar
#Add 200ml dH2O
#Autoclave

==Uses==
*[[Streptomyces Colony PCR]]
*[[Spore titres]]
*[[Re-isolation experiments]]
*[[Selection for resistance to salt-sensitive antibiotics]]

==Notes==
For growth in liquid culture, [[Difco Nutrient Broth]] can be made without the agar.

==References==