https://actinobase.org/index.php?action=history&feed=atom&title=HV
HV - Revision history
2026-10-17T22:34:22Z
Revision history for this page on the wiki
MediaWiki 1.39.3
https://actinobase.org/index.php?title=HV&diff=2369&oldid=prev
Matt Hutchings: /* Preparation */
2026-03-02T10:08:12Z
<p><span dir="auto"><span class="autocomment">Preparation</span></span></p>
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td>
<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 10:08, 2 March 2026</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>*0.01g FeSO<sub>4</sub></div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>*0.01g FeSO<sub>4</sub></div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>*1mL VB stock solution (added after autoclaving)</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>*1mL VB stock solution (added after autoclaving)</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>*<del style="font-weight: bold; text-decoration: none;">10g </del>agar</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>*<ins style="font-weight: bold; text-decoration: none;">20g </ins>agar</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>*1L dH<sub>2</sub>O </div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>*1L dH<sub>2</sub>O </div></td></tr>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>#Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. </div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>#Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. </div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>#Add 1ml VB stock solution, mix well and pour into sterile Petri dishes.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>#Add 1ml VB stock solution, mix well and pour into sterile Petri dishes.</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2" class="diff-side-added"></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>==Uses==</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>==Uses==</div></td></tr>
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Matt Hutchings
https://actinobase.org/index.php?title=HV&diff=586&oldid=prev
MFeeney: Created page with "=HV= ==Preparation== Per Litre: *1g humic acid *1.7g KCl *0.5g Na<sub>2</sub>HPO<sub>4</sub> *0.5g MgSO<sub>4</sub> *0.02g CaCO<sub>3</sub> *0.01g FeSO<sub>4</sub> *1mL VB..."
2019-07-26T14:27:18Z
<p>Created page with "=HV= ==Preparation== Per Litre: *1g humic acid *1.7g KCl *0.5g Na<sub>2</sub>HPO<sub>4</sub> *0.5g MgSO<sub>4</sub> *0.02g CaCO<sub>3</sub> *0.01g FeSO<sub>4</sub> *1mL VB..."</p>
<p><b>New page</b></p><div>=HV=<br />
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<br />
==Preparation==<br />
Per Litre:<br />
*1g humic acid<br />
*1.7g KCl<br />
*0.5g Na<sub>2</sub>HPO<sub>4</sub><br />
*0.5g MgSO<sub>4</sub><br />
*0.02g CaCO<sub>3</sub><br />
*0.01g FeSO<sub>4</sub><br />
*1mL VB stock solution (added after autoclaving)<br />
*10g agar<br />
*1L dH<sub>2</sub>O <br />
<br />
'''Instructions'''<br />
#Combine all components (except for the VB stock solution) and mix well. <br />
#Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. <br />
#Add 1ml VB stock solution, mix well and pour into sterile Petri dishes.<br />
<br />
<br />
==Uses==<br />
*[[Isolation experiments]]<br />
<br />
==Notes==<br />
VB stock solution: 50mg VB1 (thiamine), VB2 (riboflavin), VB3 (niacin), VB6 (pyridoxine), B5 (D-calcium pantothenate), inositol and PABA (para amino acid), 25mg B7 (Biotin),and 100mL distilled water). <br />
<br />
<br />
==References==<br />
Hayakawa and Nonomura (1987). Humic acid-vitamin agar, a new medium for the selective isolation of soil actinomycetes. Journal of Fermentation Technology 65(5): 501-509.</div>
MFeeney