IMA - Revision history

MFeeney at 09:11, 30 July 2019

2019-07-30T09:11:50Z

← Older revision		Revision as of 09:11, 30 July 2019
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	IMA is sometimes also called [https://en.wikipedia.org/wiki/Smash_(instant_mashed_potato) Smash] (after the brand name of the instant potato commonly used). Other brands of instant mashed potato can also be used to make up this medium.		IMA is sometimes also called [https://en.wikipedia.org/wiki/Smash_(instant_mashed_potato) Smash] (after the brand name of the instant potato commonly used). Other brands of instant mashed potato can also be used to make up this medium.

	[https://twitter.com/DavidWiddick David Widdick] initially conceived the idea of using instant mashed potatoes for the ~~cultivatioof~~ the potato pathogen ''[[Streptomyces scabies]]''. (Perhaps unsurprisingly, ''S. scabies'' does indeed grow particularly well on IMA.) The initial formulation contained 50g/L Smash and 12g/L agar<sup>[1]</sup>, but later formulations follow this recipe (20g/L Smash).<sup>[2]</sup>		[https://twitter.com/DavidWiddick David Widdick] initially conceived the idea of using instant mashed potatoes for the cultivation of the potato pathogen ''[[Streptomyces scabies]]''. (Perhaps unsurprisingly, ''S. scabies'' does indeed grow particularly well on IMA.) The initial formulation contained 50g/L Smash and 12g/L agar<sup>[1]</sup>, but later formulations follow this recipe (20g/L Smash).<sup>[2]</sup>

	IMA is also suitable for the growth of other ''Streptomyces'' sp. Strains of ''[[Streptomyces coelicolor]]'' that fail to sporulate well on [[SFM]] may sporulate better on IMA.		IMA is also suitable for the growth of other ''Streptomyces'' sp. Strains of ''[[Streptomyces coelicolor]]'' that fail to sporulate well on [[SFM]] may sporulate better on IMA.

MFeeney at 09:09, 30 July 2019

2019-07-30T09:09:31Z

← Older revision		Revision as of 09:09, 30 July 2019
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	==References==		==References==
	[1] Joshi, M. V., Mann, S. G., Antelmann, H. , Widdick, D. A., Fyans, J. K., Chandra, G. , Hutchings, M. I., Toth, I. , Hecker, M. , Loria, R. and Palmer, T. (2010), The twin arginine protein transport pathway exports multiple virulence proteins in the plant pathogen Streptomyces scabies. Molecular Microbiology, 77: 252-271. doi:10.1111/j.1365-2958.2010.07206.x		[1] Joshi, M. V., Mann, S. G., Antelmann, H. , Widdick, D. A., Fyans, J. K., Chandra, G. , Hutchings, M. I., Toth, I. , Hecker, M. , Loria, R. and Palmer, T. (2010), The twin arginine protein transport pathway exports multiple virulence proteins in the plant pathogen ''Streptomyces scabies''. Molecular Microbiology, 77: 252-271. doi:10.1111/j.1365-2958.2010.07206.x

	[2] Widdick, D. A., Hicks, M. G., Thompson, B. J., Tschumi, A. , Chandra, G. , Sutcliffe, I. C., Brülle, J. K., Sander, P. , Palmer, T. and Hutchings, M. I. (2011), Dissecting the complete lipoprotein biogenesis pathway in Streptomyces scabies. Molecular Microbiology, 80: 1395-1412. doi:10.1111/j.1365-2958.2011.07656.x		[2] Widdick, D. A., Hicks, M. G., Thompson, B. J., Tschumi, A. , Chandra, G. , Sutcliffe, I. C., Brülle, J. K., Sander, P. , Palmer, T. and Hutchings, M. I. (2011), Dissecting the complete lipoprotein biogenesis pathway in ''Streptomyces scabies''. Molecular Microbiology, 80: 1395-1412. doi:10.1111/j.1365-2958.2011.07656.x

MFeeney at 09:08, 30 July 2019

2019-07-30T09:08:07Z

← Older revision		Revision as of 09:08, 30 July 2019
Line 2:		Line 2:

	IMA is sometimes also called [https://en.wikipedia.org/wiki/Smash_(instant_mashed_potato) Smash] (after the brand name of the instant potato commonly used). Other brands of instant mashed potato can also be used to make up this medium.		IMA is sometimes also called [https://en.wikipedia.org/wiki/Smash_(instant_mashed_potato) Smash] (after the brand name of the instant potato commonly used). Other brands of instant mashed potato can also be used to make up this medium.

			[https://twitter.com/DavidWiddick David Widdick] initially conceived the idea of using instant mashed potatoes for the cultivatioof the potato pathogen ''[[Streptomyces scabies]]''. (Perhaps unsurprisingly, ''S. scabies'' does indeed grow particularly well on IMA.) The initial formulation contained 50g/L Smash and 12g/L agar<sup>[1]</sup>, but later formulations follow this recipe (20g/L Smash).<sup>[2]</sup>

			IMA is also suitable for the growth of other ''Streptomyces'' sp. Strains of ''[[Streptomyces coelicolor]]'' that fail to sporulate well on [[SFM]] may sporulate better on IMA.

	==Preparation==		==Preparation==
Line 16:		Line 20:

	==Uses==		==Uses==
	*Routine growth of Streptomyces sp.		*Routine growth of ''Streptomyces'' sp.
	*[[Preparing spore stocks]]		*[[Preparing spore stocks]]

	==Notes==		==Notes==
	*Because the agar and instant mashed potato are not very soluble, IMA should be made up in small 100 mL batches (do not attempt to make up 1 L and aliquot into flasks for autoclaving)		*Because the agar and instant mashed potato are not very soluble, IMA should be made up in small 100 mL batches (do not attempt to make up 1 L and aliquot into flasks for autoclaving)
	*''[[Streptomyces scabies]]'' grows particularly well on IMA.
	*Strains of ''[[Streptomyces coelicolor]]'' that fail to sporulate well on [[SFM]] may sporulate better on IMA.

	==References==		==References==
			[1] Joshi, M. V., Mann, S. G., Antelmann, H. , Widdick, D. A., Fyans, J. K., Chandra, G. , Hutchings, M. I., Toth, I. , Hecker, M. , Loria, R. and Palmer, T. (2010), The twin arginine protein transport pathway exports multiple virulence proteins in the plant pathogen Streptomyces scabies. Molecular Microbiology, 77: 252-271. doi:10.1111/j.1365-2958.2010.07206.x

			[2] Widdick, D. A., Hicks, M. G., Thompson, B. J., Tschumi, A. , Chandra, G. , Sutcliffe, I. C., Brülle, J. K., Sander, P. , Palmer, T. and Hutchings, M. I. (2011), Dissecting the complete lipoprotein biogenesis pathway in Streptomyces scabies. Molecular Microbiology, 80: 1395-1412. doi:10.1111/j.1365-2958.2011.07656.x

MFeeney at 08:24, 30 July 2019

2019-07-30T08:24:17Z

← Older revision		Revision as of 08:24, 30 July 2019
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	=IMA(Instant Mash Agar)=		=IMA (Instant Mash Agar)=

	IMA is sometimes also called [https://en.wikipedia.org/wiki/Smash_(instant_mashed_potato) Smash] (after the brand name of the instant potato commonly used). Other brands of instant mashed potato can also be used to make up this medium.		IMA is sometimes also called [https://en.wikipedia.org/wiki/Smash_(instant_mashed_potato) Smash] (after the brand name of the instant potato commonly used). Other brands of instant mashed potato can also be used to make up this medium.
Line 17:		Line 17:
	==Uses==		==Uses==
	*Routine growth of Streptomyces sp.		*Routine growth of Streptomyces sp.
	*[Preparing spore stocks]]		*[[Preparing spore stocks]]

	==Notes==		==Notes==
	*Because the agar and instant mashed potato are not very soluble, IMA should be made up in small 100 mL batches (do not attempt to make up 1 L and aliquot into flasks for autoclaving)		*Because the agar and instant mashed potato are not very soluble, IMA should be made up in small 100 mL batches (do not attempt to make up 1 L and aliquot into flasks for autoclaving)
	*[[''Streptomyces scabies'']] grows particularly well on IMA.		*''[[Streptomyces scabies]]'' grows particularly well on IMA.
	*Strains of [[''Streptomyces coelicolor'']] that fail to sporulate ~~very~~ well on [[SFM]] may sporulate better on IMA.		*Strains of ''[[Streptomyces coelicolor]]'' that fail to sporulate well on [[SFM]] may sporulate better on IMA.

	==References==		==References==

MFeeney at 08:21, 30 July 2019

2019-07-30T08:21:44Z

← Older revision		Revision as of 08:21, 30 July 2019
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	==Notes==		==Notes==
	*Because the agar and instant mashed potato are not very soluble, IMA should be made up in small 100 mL batches (do not attempt to make up 1 L and aliquot into flasks for autoclaving)		*Because the agar and instant mashed potato are not very soluble, IMA should be made up in small 100 mL batches (do not attempt to make up 1 L and aliquot into flasks for autoclaving)
	*[[''Streptomyces scabies''] grows particularly well on IMA.		*[[''Streptomyces scabies'']] grows particularly well on IMA.
	*Strains of [[''Streptomyces coelicolor''] that fail to sporulate very well on [[SFM]] may sporulate better on IMA.		*Strains of [[''Streptomyces coelicolor'']] that fail to sporulate very well on [[SFM]] may sporulate better on IMA.

	==References==		==References==

MFeeney: Created page with "=IMA(Instant Mash Agar)= IMA is sometimes also called [https://en.wikipedia.org/wiki/Smash_(instant_mashed_potato) Smash] (after the brand name of the instant potato commonly..."

2019-07-30T08:21:07Z

Created page with "=IMA(Instant Mash Agar)= IMA is sometimes also called [https://en.wikipedia.org/wiki/Smash_(instant_mashed_potato) Smash] (after the brand name of the instant potato commonly..."

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=IMA(Instant Mash Agar)=

IMA is sometimes also called [https://en.wikipedia.org/wiki/Smash_(instant_mashed_potato) Smash] (after the brand name of the instant potato commonly used). Other brands of instant mashed potato can also be used to make up this medium.

==Preparation==
Per 100 mL:
*2g Smash instant potato
*2g agar
*100mL tap water

'''Instructions'''
#Mix all the components together well in a 200mL Erlenmeyer flask with a bung, or other container suitable for autoclaving.
#Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
#Mix well and pour into sterile Petri dishes.

==Uses==
*Routine growth of Streptomyces sp.
*[Preparing spore stocks]]

==Notes==
*Because the agar and instant mashed potato are not very soluble, IMA should be made up in small 100 mL batches (do not attempt to make up 1 L and aliquot into flasks for autoclaving)
*[[''Streptomyces scabies''] grows particularly well on IMA.
*Strains of [[''Streptomyces coelicolor''] that fail to sporulate very well on [[SFM]] may sporulate better on IMA.

==References==