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ISP4 - Revision history
2026-10-17T23:42:42Z
Revision history for this page on the wiki
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https://actinobase.org/index.php?title=ISP4&diff=2345&oldid=prev
Matt Hutchings: /* Preparation */
2025-10-16T13:23:12Z
<p><span dir="auto"><span class="autocomment">Preparation</span></span></p>
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td>
<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 13:23, 16 October 2025</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>==Preparation==</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>==Preparation==</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>*10 g Soluble Starch</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>*10 g Soluble Starch</div></td></tr>
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Matt Hutchings
https://actinobase.org/index.php?title=ISP4&diff=619&oldid=prev
DaveMark: Created page with "=ISP 4= International <i>Streptomyces</i> Project medium 4, also named inorganic salts-starch agar ==Preparation== *10 g Soluble Starch *1 g MgSO<sub>4</sub> x 7H<sub>2</sub>..."
2019-07-26T15:22:43Z
<p>Created page with "=ISP 4= International <i>Streptomyces</i> Project medium 4, also named inorganic salts-starch agar ==Preparation== *10 g Soluble Starch *1 g MgSO<sub>4</sub> x 7H<sub>2</sub>..."</p>
<p><b>New page</b></p><div>=ISP 4=<br />
International <i>Streptomyces</i> Project medium 4, also named inorganic salts-starch agar<br />
<br />
==Preparation==<br />
*10 g Soluble Starch<br />
*1 g MgSO<sub>4</sub> x 7H<sub>2</sub>O<br />
*1 g NaCl<br />
*2 g (NH<sub>4</sub>)<sub>2</sub>SO<sub>4</sub><br />
*2 g CaCO<sub>3</sub><br />
*1 L dH<sub>2</sub><br />
*1 mL Trace Salts solution<br />
**0.1 g FeSO<sub>4</sub> x 7H<sub>2</sub>O<br />
**0.1 g MnCl<sub>2</sub> x 4H<sub>2</sub>O<br />
**0.1 g ZnSO<sub>4</sub> x 7H<sub>2</sub>O<br />
**100 mL dH<sub>2</sub>O<br />
*20 g agar<br />
<br />
<br />
'''Instructions'''<br />
<br />
Prepare as two separate solutions and mix before adding agar and autoclaving<br />
<br />
===Solution 1===<br />
#Make a paste of the soluble starch with a small volume of cold dH<sub>2</sub>O<br />
#Bring the paste up to a volume of 500 mL<br />
===Solution 2===<br />
#Dissolve the remaining constituents and trace salts solution in 500 mL dH<sub>2</sub>O<br />
#Adjust the pH so that it's between 7 and 7.4<br />
<br />
==Uses==<br />
*Screening for antibiotic production<br />
<br />
==Notes==<br />
<br />
<br />
==References==</div>
DaveMark