Indicator compounds - Revision history

MFeeney at 16:13, 28 February 2020

2020-02-28T16:13:13Z

← Older revision		Revision as of 16:13, 28 February 2020
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	=Indicator Compounds=		=Indicator Compounds=

	Indicator compounds can be used to measure the activity of enzymes such as β-galactosidase or β-glucuronidase. These compounds are usually added directly to the bacterial growth medium and provide a qualitative measure of enzyme activity via a colorimetric change. (For example, the colourless compound 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-gal) is converted into a dark blue product by a β-galactosidase.		Indicator compounds can be used to measure the activity of enzymes such as β-galactosidase or β-glucuronidase. These compounds are usually added directly to the bacterial growth medium and provide a qualitative measure of enzyme activity via a colorimetric change. (For example, the colourless compound 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-gal) is converted into a dark blue product by a β-galactosidase; similarly, colourless 5-Bromo-4-chloro-1H-indol-3-yl β-D-glucopyranosiduronic acid (X-Gluc) (X-gal) is converted into a dark blue product by a β-glucuronidase.)

			These compounds can be used to measure native enzyme activity, but are perhaps most commonly used with [[reporter genes]], where the promoter or gene of interest is fused to the gene encoding the enzyme (i.e. β-galactosidase or β-glucuronidase).

			A number of different reporter systems have been tried in <i>Streptomyces</i>: the most commonly used is perhaps the β-glucuronidase Gus.<sup>[1]</sup>

	==5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-Gal)==		==5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-Gal)==
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	'''Uses'''		'''Uses'''
	*Detection of β-galactosidase activity on solid media		*Detection of β-galactosidase activity on solid media
	*Bacterial two-hybrid analysis~~<sup>~~[1]~~</sup>~~		*[[Bacterial two-hybrid analysis]]
			*[[Blue-white screening]]

	'''Instructions for use'''		'''Instructions for use'''

MFeeney at 15:55, 28 February 2020

2020-02-28T15:55:57Z

← Older revision		Revision as of 15:55, 28 February 2020
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	=Indicator Compounds=		=Indicator Compounds=

			Indicator compounds can be used to measure the activity of enzymes such as β-galactosidase or β-glucuronidase. These compounds are usually added directly to the bacterial growth medium and provide a qualitative measure of enzyme activity via a colorimetric change. (For example, the colourless compound 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-gal) is converted into a dark blue product by a β-galactosidase.

	==5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-Gal)==		==5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-Gal)==

MFeeney: /* 5-Bromo-4-chloro-1'H'-indol-3-yl β-D-glucopyranosiduronic acid (X-Gluc) */

2020-02-28T15:11:04Z

5-Bromo-4-chloro-1'H'-indol-3-yl β-D-glucopyranosiduronic acid (X-Gluc)

← Older revision		Revision as of 15:11, 28 February 2020
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	==5-Bromo-4-chloro-~~1'H'~~-indol-3-yl β-D-glucopyranosiduronic acid (X-Gluc)==		==5-Bromo-4-chloro-1H-indol-3-yl β-D-glucopyranosiduronic acid (X-Gluc)==

	'''Uses'''		'''Uses'''
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	'''Instructions for use'''		'''Instructions for use'''
	#C		#C



	==Notes==		==Notes==

MFeeney: Created page with "=Indicator Compounds= ==5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-Gal)== '''Uses''' Detection of β-galactosidase activity on solid media Bacterial two-hybrid a..."

2020-02-28T15:09:55Z

Created page with "=Indicator Compounds= ==5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-Gal)== '''Uses''' *Detection of β-galactosidase activity on solid media *Bacterial two-hybrid a..."

New page

=Indicator Compounds=

==5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-Gal)==

'''Uses'''
*Detection of β-galactosidase activity on solid media
*Bacterial two-hybrid analysis<sup>[1]</sup>

'''Instructions for use'''

==5-Bromo-4-chloro-1'H'-indol-3-yl β-D-glucopyranosiduronic acid (X-Gluc)==

'''Uses'''
*Detection of β-glucuronidase activity on solid media
*Screening for β-glucuronidase -positive or -negative candidates<sup>[1]</sup>

'''Instructions for use'''
#C

==Notes==

==References==

[1] Myronovskyi, M., Welle, E., Fedorenko, V., & Luzhetskyy, A. (2011). Beta-glucuronidase as a sensitive and versatile reporter in actinomycetes. Applied and environmental microbiology, 77(15), 5370–5383. https://doi.org/10.1128/AEM.00434-11

Indicator compounds - Revision history

MFeeney at 16:13, 28 February 2020

MFeeney at 15:55, 28 February 2020

MFeeney: /* 5-Bromo-4-chloro-1'H'-indol-3-yl β-D-glucopyranosiduronic acid (X-Gluc) */

MFeeney: Created page with "=Indicator Compounds= ==5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-Gal)== '''Uses''' *Detection of β-galactosidase activity on solid media *Bacterial two-hybrid a..."

MFeeney: Created page with "=Indicator Compounds= ==5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-Gal)== '''Uses''' Detection of β-galactosidase activity on solid media Bacterial two-hybrid a..."