https://actinobase.org/index.php?action=history&feed=atom&title=Isolating_Actinobacteria_from_soil_environments 2026-10-18T00:32:36Z Revision history for this page on the wiki MediaWiki 1.39.3 https://actinobase.org/index.php?title=Isolating_Actinobacteria_from_soil_environments&diff=1485&oldid=prev 2019-10-25T14:20:17Z

<p></p> <table style="background-color: #fff; color: #202122;" data-mw="interface"> <col class="diff-marker" /> <col class="diff-content" /> <col class="diff-marker" /> <col class="diff-content" /> <tr class="diff-title" lang="en-GB"> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 14:20, 25 October 2019</td> </tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l1">Line 1:</td> <td colspan="2" class="diff-lineno">Line 1:</td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>Isolating Actinobacteria from soil environments</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">=</ins>Isolating Actinobacteria from soil environments<ins style="font-weight: bold; text-decoration: none;">=</ins></div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>=Equipment &amp; Materials=</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>=Equipment &amp; Materials=</div></td></tr> </table>

DaveMark https://actinobase.org/index.php?title=Isolating_Actinobacteria_from_soil_environments&diff=1484&oldid=prev 2019-10-25T14:19:57Z

<p>Created page with &quot;Isolating Actinobacteria from soil environments =Equipment &amp; Materials= *Sterile containers to transport the soil *A heat block *Plates of Isolation media *A vortexer *¼ Str...&quot;</p> <p><b>New page</b></p><div>Isolating Actinobacteria from soil environments<br /> <br /> =Equipment &amp; Materials=<br /> *Sterile containers to transport the soil<br /> *A heat block<br /> *Plates of Isolation media<br /> *A vortexer<br /> *¼ Strength Ringer’s Solution <br /> *Nalidixic acid, cycloheximide, and nystatin<br /> <br /> =Preparation of Soil=<br /> #Incubate 1g of soil at 50oC for 1 hour in order to kill vegetative organisms<br /> #Suspend the soil in 10 mL ¼ Strength Ringer’s and vortex for 10 mins in order to thoroughly suspend your sample<br /> #Dilute your soil suspension 1/10 and 1/100 and spread 200 µL of these onto plates of your isolation media supplemented with 25 µg mL-1 nystatin, 25 µg mL-1 cycloheximide, and 10 µg ml-1 nalidixic acid<br /> #Incubate your plates at 30oC for three weeks, the temperature and duration will vary depending on what you are aiming to isolate<br /> #Select actinobacterial colonies that have grown and patch them onto the same agar in order to isolate the strains</div>

DaveMark