LisAlgora: Created page with "=Lambda HindIII Marker Preparation= Lambda (λ) DNA (500 µg/ml): 100µl 10x Reaction Buffer: 45 µl HindIII or PstI(10U/µl): 5µl ddWater: 295µl Preparation: #Incubate..."

2020-04-23T22:37:51Z

Created page with "=Lambda HindIII Marker Preparation= *Lambda (λ) DNA (500 µg/ml): 100µl *10x Reaction Buffer: 45 µl *HindIII or PstI(10U/µl): 5µl *ddWater: 295µl Preparation: #Incubate..."

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=Lambda HindIII Marker Preparation=
*Lambda (λ) DNA (500 µg/ml): 100µl
*10x Reaction Buffer: 45 µl
*HindIII or PstI(10U/µl): 5µl
*ddWater: 295µl

Preparation:
#Incubate 90 minutes 37°C
#Add 5µl HindIII or PstI
#Incubate 90 minutes 37°
#Incubate 10 minutes, 75°C
#Store at –20°C

Add 6x loading dye before loading into gel

=Size and Concentration of Bands=
{| class="wikitable"
! style="font-weight:bold; font-family:Arial, Helvetica, sans-serif !important;;" | Size of Bands in bp (λ HindIII)
! style="font-weight:bold;" | ng in 5µl
|-
| style="font-family:Arial, Helvetica, sans-serif !important;;" | (27491)
| -
|-
| style="font-family:Arial, Helvetica, sans-serif !important;;" | (23130)
| 240
|-
| style="font-family:Arial, Helvetica, sans-serif !important;;" | 9416
| 97
|-
| style="font-family:Arial, Helvetica, sans-serif !important;;" | 6556
| 68
|-
| style="font-family:Arial, Helvetica, sans-serif !important;;" | (4361)
| 44
|-
| style="font-family:Arial, Helvetica, sans-serif !important;;" | 2322
| 24
|-
| style="font-family:Arial, Helvetica, sans-serif !important;;" | 2027
| 21
|-
| style="font-family:Arial, Helvetica, sans-serif !important;;" | 561
| 6
|-
| style="font-family:Arial, Helvetica, sans-serif !important;;" | 125
| -
|}

Lambda DNA/HindIII Ladder - Revision history

LisAlgora: Created page with "=Lambda HindIII Marker Preparation= *Lambda (λ) DNA (500 µg/ml): 100µl *10x Reaction Buffer: 45 µl *HindIII or PstI(10U/µl): 5µl *ddWater: 295µl Preparation: #Incubate..."

LisAlgora: Created page with "=Lambda HindIII Marker Preparation= Lambda (λ) DNA (500 µg/ml): 100µl 10x Reaction Buffer: 45 µl HindIII or PstI(10U/µl): 5µl ddWater: 295µl Preparation: #Incubate..."