Tommclean at 15:26, 26 September 2019

2019-09-26T15:26:28Z

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	The -NaCl (no salt) version of Lennox should be used when using salt sensitive antibiotics like [[hygromycin]]: for example, when cloning into [[pSS170]]. (Alternatively, [[DNA]] or [[DNB]] can be used for hygromycin selection.)		The -NaCl (no salt) version of Lennox should be used when using salt sensitive antibiotics like [[hygromycin]]: for example, when cloning into [[pSS170]]. (Alternatively, [[DNA]] or [[DNB]] can be used for hygromycin selection.)

	To make liquid LB medium, omit the agar and autoclave as above.		To make liquid Lennox medium, omit the agar and autoclave as above.

Tommclean: Created page with "=Lennox Broth= Known as Lennox Broth (or sometimes, mistakenly, as Luria-Bertani medium), this rich medium is used routinely for growth of Escherichia coli. There are..."

2019-09-26T15:23:45Z

Created page with "=Lennox Broth= Known as Lennox Broth (or sometimes, mistakenly, as Luria-Bertani medium), this rich medium is used routinely for growth of Escherichia coli. There are..."

New page

=Lennox Broth=
Known as Lennox Broth (or sometimes, mistakenly, as Luria-Bertani medium), this rich medium is used routinely for growth of Escherichia coli. There are several variants on this medium, with differing amounts of NaCl; glucose may also be added, as in the original recipe (Bertani 1951).

==Preparation==
Per litre:
*5g Yeast Extract
*5g NaCl
*10g Tryptone
*Up to 1L dH2O

'''Instructions'''
#Add 5g Yeast Extract, 5g NaCl and 10g Tryptone to a beaker
#Add 800ml dH2O and stir until all solids are fully suspended
#Make up to 1L with dH2O
#For solid media, add 4g agar per 200ml LB
#Autoclave

===-NaCl Lennox===
Per litre:
*5g Yeast Extract
*10g Tryptone
*Up to 1L dH2O

==Uses==
*Routine growth of Escherichia coli
*Checking for contamination in Streptomyces spore stocks or cultures

==Notes==
This is the Lennox formulation, with 5g/L NaCl. Alternatively, 10g/L NaCl can be used (Miller formulation) or no salt (-NaCl).
The -NaCl (no salt) version of Lennox should be used when using salt sensitive antibiotics like [[hygromycin]]: for example, when cloning into [[pSS170]]. (Alternatively, [[DNA]] or [[DNB]] can be used for hygromycin selection.)

To make liquid LB medium, omit the agar and autoclave as above.

==References==
Lennox, E. S. 1955. Transduction of linked genetic characters of the host by bacteriophage P1.
Virology. 1:190-206.

Lennox - Revision history

Tommclean at 15:26, 26 September 2019

Tommclean: Created page with "=Lennox Broth= Known as Lennox Broth (or sometimes, mistakenly, as Luria-Bertani medium), this rich medium is used routinely for growth of Escherichia coli. There are..."