Tommclean: Created page with "=Luria Broth= Known as Luria Broth (or sometimes, mistakenly, as LB or Lennox medium), this rich medium is used routinely for growth of Escherichia coli. There are se..."

2019-09-26T15:25:58Z

Created page with "=Luria Broth= Known as Luria Broth (or sometimes, mistakenly, as LB or Lennox medium), this rich medium is used routinely for growth of Escherichia coli. There are se..."

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=Luria Broth=
Known as Luria Broth (or sometimes, mistakenly, as LB or Lennox medium), this rich medium is used routinely for growth of Escherichia coli. There are several variants on this medium, with differing amounts of NaCl; glucose may also be added, as in the original recipe (Bertani 1951).

==Preparation==
Per litre:
*5g Yeast Extract
*0.5g NaCl
*10g Tryptone
*Up to 1L dH2O

'''Instructions'''
#Add 5g Yeast Extract, 0.5g NaCl and 10g Tryptone to a beaker
#Add 800ml dH2O and stir until all solids are fully suspended
#Make up to 1L with dH2O
#For solid media, add 4g agar per 200ml LB
#Autoclave

===-NaCl Lennox===
Per litre:
*5g Yeast Extract
*10g Tryptone
*Up to 1L dH2O

==Uses==
*Routine growth of Escherichia coli
*Checking for contamination in Streptomyces spore stocks or cultures

==Notes==
This is the Luria formulation, with 0.5g/L NaCl. Alternatively, 10g/L NaCl can be used (Miller formulation) or no salt (-NaCl).
The -NaCl (no salt) version of Luria should be used when using salt sensitive antibiotics like [[hygromycin]]: for example, when cloning into [[pSS170]]. (Alternatively, [[DNA]] or [[DNB]] can be used for hygromycin selection.)

To make liquid Luria medium, omit the agar and autoclave as above.

==References==
Luria, S. E., and J. W. Burrous. 1955. Hybridization between Escherichia coli and Shigella. J.
Bacteriol. 74:461-476.

Luria - Revision history

Tommclean: Created page with "=Luria Broth= Known as Luria Broth (or sometimes, mistakenly, as LB or Lennox medium), this rich medium is used routinely for growth of Escherichia coli. There are se..."