MM - Revision history

Matt Hutchings: /* Preparation */

2026-03-02T10:10:36Z

Preparation

← Older revision		Revision as of 10:10, 2 March 2026
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	0.01g FeSO<sub>4</sub>7H<sub>2</sub>O		0.01g FeSO<sub>4</sub>7H<sub>2</sub>O
	*10g glucose (added after autoclaving)		*10g glucose (added after autoclaving)
	*~~10g~~ agar		*20g agar
	*1L dH<sub>2</sub>O		*1L dH<sub>2</sub>O

Test at 15:48, 4 July 2019

2019-07-04T15:48:51Z

← Older revision		Revision as of 15:48, 4 July 2019
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	==Uses==		==Uses==
	*~~studies~~ of auxotrophic mutants and carbon source utilization		*Studies of auxotrophic mutants and carbon source utilization.

	==Notes==		==Notes==

MFeeney at 12:51, 2 July 2019

2019-07-02T12:51:26Z

← Older revision		Revision as of 12:51, 2 July 2019
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	*0.5g K<sub>2</sub>HPO<sub>4</sub>		*0.5g K<sub>2</sub>HPO<sub>4</sub>
	0.2g MgSO<sub>4</sub>7H<sub>2</sub>O		0.2g MgSO<sub>4</sub>7H<sub>2</sub>O
	0.01g ~~FeSO4~~~~7H2O~~		0.01g FeSO<sub>4</sub>7H<sub>2</sub>O
	*10g glucose (added after autoclaving)		*10g glucose (added after autoclaving)
	*10g agar		*10g agar
	*1L ~~dH2O~~		*1L dH<sub>2</sub>O

	'''Instructions'''		'''Instructions'''
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	The glucose can be replaced by alternative carbon sources such as glycerol, arabinose, or mannitol (each at 5g/L). Mannitol is often preferable to glucose for optimal growth and development.		The glucose can be replaced by alternative carbon sources such as glycerol, arabinose, or mannitol (each at 5g/L). Mannitol is often preferable to glucose for optimal growth and development.

	To study carbon source utilization, the L-asparagine should be replaced by ammonium sulfate (5 g/L(~~NH4~~)~~2SO4~~) - to avoid confusion due to utilization of asparagine as a carbon source.		To study carbon source utilization, the L-asparagine should be replaced by ammonium sulfate (5 g/L(NH<sub>4</sub>)2SO<sub>4</sub>) - to avoid confusion due to utilization of asparagine as a carbon source.

	Additional supplements can be added as follows (amount/L): amino acids except histidine, 50 mg; histidine, 70 mg; adenine and uracil, 10 mg; vitamins, 1 mg; dihydrostreptomycin sulfate, 50 mg; acriflavine, 15 mg. (Hopwood 1967)		Additional supplements can be added as follows (amount/L): amino acids except histidine, 50 mg; histidine, 70 mg; adenine and uracil, 10 mg; vitamins, 1 mg; dihydrostreptomycin sulfate, 50 mg; acriflavine, 15 mg. (Hopwood 1967)

MFeeney at 12:03, 2 July 2019

2019-07-02T12:03:26Z

← Older revision		Revision as of 12:03, 2 July 2019
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	Per litre:		Per litre:
	*0.5g L-asparagine		*0.5g L-asparagine
	*0.5g ~~K2HPO4~~		*0.5g K<sub>2</sub>HPO<sub>4</sub>
	0.2g ~~MgSO4~~~~7H2O~~		0.2g MgSO<sub>4</sub>7H<sub>2</sub>O
	0.01g FeSO47H2O		0.01g FeSO47H2O
	*10g glucose (added after autoclaving)		*10g glucose (added after autoclaving)

ActinoCommittee at 13:23, 26 June 2019

2019-06-26T13:23:09Z

← Older revision		Revision as of 13:23, 26 June 2019
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	=MM (Minimal Medium)=		=MM (Minimal Medium)=
	MM is a minimal medium suitable for growth of many <em>Streptomyces</em> sp. It is often made up with glucose or mannitol, but other carbon sources can be used. It can be further supplemented with additional growth factors as described below.		MM is a minimal medium suitable for growth of many <em>Streptomyces</em> sp (<em>S. coelicolor</em> and <em>S. venezuelae</em> both grow well on it). It is often made up with glucose or mannitol, but other carbon sources can be used. It can be further supplemented with additional growth factors as described below.

	==Preparation==		==Preparation==
Line 10:		Line 10:
	*10g glucose (added after autoclaving)		*10g glucose (added after autoclaving)
	*10g agar		*10g agar
	*=1L dH2O		*1L dH2O

	'''Instructions'''		'''Instructions'''
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	#Dispense 200ml into flasks each containing 2g agar		#Dispense 200ml into flasks each containing 2g agar
	#Autoclave		#Autoclave
	#After autoclaving, add 4 ml sterile 50% glucose solution to each flask		#After autoclaving, add 4 ml sterile 50% glucose solution (autoclaved separately or filter-sterilized) to each flask
	#Add any other growth factors required for auxotrophic mutants		#Add any other growth factors required for auxotrophic mutants

ActinoCommittee at 13:21, 26 June 2019

2019-06-26T13:21:11Z

← Older revision		Revision as of 13:21, 26 June 2019
Line 1:		Line 1:
	=MM Medium=		=MM (Minimal Medium)=
			MM is a minimal medium suitable for growth of many <em>Streptomyces</em> sp. It is often made up with glucose or mannitol, but other carbon sources can be used. It can be further supplemented with additional growth factors as described below.

	==Preparation==		==Preparation==
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	==Uses==		==Uses==
			*studies of auxotrophic mutants and carbon source utilization

	==Notes==		==Notes==
			The glucose can be replaced by alternative carbon sources such as glycerol, arabinose, or mannitol (each at 5g/L). Mannitol is often preferable to glucose for optimal growth and development.

			To study carbon source utilization, the L-asparagine should be replaced by ammonium sulfate (5 g/L(NH4)2SO4) - to avoid confusion due to utilization of asparagine as a carbon source.

			Additional supplements can be added as follows (amount/L): amino acids except histidine, 50 mg; histidine, 70 mg; adenine and uracil, 10 mg; vitamins, 1 mg; dihydrostreptomycin sulfate, 50 mg; acriflavine, 15 mg. (Hopwood 1967)

	==References==		==References==
	Hopwood DA. Genetic analysis and genome structure in Streptomyces coelicolor. Bacteriol Rev. 1967;31(4):373–403.		Hopwood DA. Genetic analysis and genome structure in Streptomyces coelicolor. Bacteriol Rev. 1967;31(4):373–403.

			Kieser, T., Bibb, M.J., Buttner, M.J., Chater, K.F. and Hopwood, D.A. (2000) Practical Streptomyces Genetics: John Innes Foundation, Norwich Research Park, Colney, Norwich NR4 7UH, UK

ActinoCommittee: Created page with "=MM Medium= ==Preparation== Per litre: 0.5g L-asparagine 0.5g K2HPO4 0.2g MgSO47H2O 0.01g FeSO47H2O 10g glucose (added after autoclaving) 10g agar *=1L dH2O '''Instr..."

2019-06-26T11:50:42Z

Created page with "=MM Medium= ==Preparation== Per litre: *0.5g L-asparagine *0.5g K2HPO4 *0.2g MgSO4*7H2O *0.01g FeSO4*7H2O *10g glucose (added after autoclaving) *10g agar *=1L dH2O '''Instr..."

New page

=MM Medium=

==Preparation==
Per litre:
*0.5g L-asparagine
*0.5g K2HPO4
*0.2g MgSO4*7H2O
*0.01g FeSO4*7H2O
*10g glucose (added after autoclaving)
*10g agar
*=1L dH2O

'''Instructions'''
#Dissolve all of the ingredients except the agar and the glucose in the distilled water
#Adjust the pH to 7.0-7.2
#Dispense 200ml into flasks each containing 2g agar
#Autoclave
#After autoclaving, add 4 ml sterile 50% glucose solution to each flask
#Add any other growth factors required for auxotrophic mutants

==Uses==

==Notes==

==References==
Hopwood DA. Genetic analysis and genome structure in Streptomyces coelicolor. Bacteriol Rev. 1967;31(4):373–403.