MYM - Revision history

Tommclean at 15:07, 26 September 2019

2019-09-26T15:07:23Z

← Older revision		Revision as of 15:07, 26 September 2019
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	#For solid medium, add 2g agar & 100mL liquid MYM to each flask		#For solid medium, add 2g agar & 100mL liquid MYM to each flask
	#Autoclave, expect a precipitate to form afterward.		#Autoclave, expect a precipitate to form afterward.
	#For optimal ''[[Streptomyces venezuelae]]'' sporulation, add [[~~trace elements~~]] at a 1:500 dilution.		#For optimal ''[[Streptomyces venezuelae]]'' sporulation, add [[Trace element solution]] at a 1:500 dilution.

	==Uses==		==Uses==

Sam Prudence: /* MYM Medium */

2019-08-15T16:20:21Z

MYM Medium

← Older revision		Revision as of 16:20, 15 August 2019
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	=MYM ~~Medium~~=		=Maltose Yeast Extract Medium (MYM)=

	Maltose-Yeast Extract-Malt Extract (MYM) medium is a rich medium used especially for the growth of ''[[Streptomyces venezuelae]]'', as it sporulates to >90% completion in liquid MYM <sup>[2]</sup>. Other <em>Streptomyces</em> sp. may also grow well on this medium.		Maltose-Yeast Extract-Malt Extract (MYM) medium is a rich medium used especially for the growth of ''[[Streptomyces venezuelae]]'', as it sporulates to >90% completion in liquid MYM <sup>[2]</sup>. Other <em>Streptomyces</em> sp. may also grow well on this medium.

Test at 15:47, 4 July 2019

2019-07-04T15:47:10Z

← Older revision		Revision as of 15:47, 4 July 2019
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	=MYM Medium=		=MYM Medium=

	Maltose-Yeast Extract-Malt Extract (MYM) medium is a rich medium used especially for the growth of ''[[Streptomyces venezuelae]]'', as it sporulates to >90% completion in liquid MYM <sup>[1]</sup>. Other <em>Streptomyces</em> sp. may also grow well on this medium.		Maltose-Yeast Extract-Malt Extract (MYM) medium is a rich medium used especially for the growth of ''[[Streptomyces venezuelae]]'', as it sporulates to >90% completion in liquid MYM <sup>[2]</sup>. Other <em>Streptomyces</em> sp. may also grow well on this medium.

	==Preparation==		==Preparation==
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	==References==		==References==
	Stuttard C. 1982. Temperate phages of Streptomyces venezuelae: lysogeny and host specificity shown by phages SV1 and SV2. Microbiology 128:115–121.		1. Stuttard C. 1982. Temperate phages of Streptomyces venezuelae: lysogeny and host specificity shown by phages SV1 and SV2. Microbiology 128:115–121.

	1. Bibb MJ, Domonkos A, Chandra G, Buttner MJ. 2012. Expression of the chaplin and rodlin hydrophobic sheath proteins in Streptomyces venezuelae is controlled by σBldN and a cognate anti-sigma factor, RsbN. Mol. Microbiol. 84:1033–1049		2. Bibb MJ, Domonkos A, Chandra G, Buttner MJ. 2012. Expression of the chaplin and rodlin hydrophobic sheath proteins in Streptomyces venezuelae is controlled by σBldN and a cognate anti-sigma factor, RsbN. Mol. Microbiol. 84:1033–1049

Test at 15:46, 4 July 2019

2019-07-04T15:46:27Z

← Older revision		Revision as of 15:46, 4 July 2019
Line 1:		Line 1:
	=MYM Medium=		=MYM Medium=

	Maltose-Yeast Extract-Malt Extract (MYM) medium is a rich medium used especially for the growth of ''[[Streptomyces venezuelae]]'', as it sporulates to >90% completion in liquid MYM ~~(Bibb et al 2012)~~. Other <em>Streptomyces</em> sp. may also grow well on this medium.		Maltose-Yeast Extract-Malt Extract (MYM) medium is a rich medium used especially for the growth of ''[[Streptomyces venezuelae]]'', as it sporulates to >90% completion in liquid MYM <sup>[1]</sup>. Other <em>Streptomyces</em> sp. may also grow well on this medium.

	==Preparation==		==Preparation==
Line 30:		Line 30:
	Stuttard C. 1982. Temperate phages of Streptomyces venezuelae: lysogeny and host specificity shown by phages SV1 and SV2. Microbiology 128:115–121.		Stuttard C. 1982. Temperate phages of Streptomyces venezuelae: lysogeny and host specificity shown by phages SV1 and SV2. Microbiology 128:115–121.

	Bibb MJ, Domonkos A, Chandra G, Buttner MJ. 2012. Expression of the chaplin and rodlin hydrophobic sheath proteins in Streptomyces venezuelae is controlled by σBldN and a cognate anti-sigma factor, RsbN. Mol. Microbiol. 84:1033–1049		1. Bibb MJ, Domonkos A, Chandra G, Buttner MJ. 2012. Expression of the chaplin and rodlin hydrophobic sheath proteins in Streptomyces venezuelae is controlled by σBldN and a cognate anti-sigma factor, RsbN. Mol. Microbiol. 84:1033–1049

Test at 15:44, 4 July 2019

2019-07-04T15:44:38Z

← Older revision		Revision as of 15:44, 4 July 2019
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	==Uses==		==Uses==
	*~~routine~~ growth of ''[[Streptomyces venezuelae]]''		*Routine growth of ''[[Streptomyces venezuelae]]''
	*~~developmental~~ studies on ''[[Streptomyces venezuelae]]''		*Developmental studies on ''[[Streptomyces venezuelae]]''

	==Notes==		==Notes==

MFeeney at 12:53, 2 July 2019

2019-07-02T12:53:26Z

← Older revision		Revision as of 12:53, 2 July 2019
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	'''Instructions'''		'''Instructions'''

	#Dissolve all ingredients in 500ml tap water & 250ml ~~dH2O~~		#Dissolve all ingredients in 500ml tap water & 250ml dH<sub>2</sub>O
	#pH to 7.2-7.3 with NaOH		#pH to 7.2-7.3 with NaOH
	#add dH<sub>2</sub>O up to 1L		#add dH<sub>2</sub>O up to 1L

MFeeney at 12:53, 2 July 2019

2019-07-02T12:53:04Z

← Older revision		Revision as of 12:53, 2 July 2019
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	*10g Malt Extract		*10g Malt Extract
	*500ml Tap Water		*500ml Tap Water
	*500ml ~~dH2O~~		*500ml dH<sub>2</sub>O

	'''Instructions'''		'''Instructions'''
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	#Dissolve all ingredients in 500ml tap water & 250ml dH2O		#Dissolve all ingredients in 500ml tap water & 250ml dH2O
	#pH to 7.2-7.3 with NaOH		#pH to 7.2-7.3 with NaOH
	#add ~~dH2O~~ up to 1L		#add dH<sub>2</sub>O up to 1L
	#For solid medium ~~add~~ add 2g agar & 100mL liquid MYM to each flask		#For solid medium, add 2g agar & 100mL liquid MYM to each flask
	#Autoclave, expect a precipitate to form afterward.		#Autoclave, expect a precipitate to form afterward.
	#For optimal ''[[Streptomyces venezuelae]]'' sporulation, add [[trace elements]] at a 1:500 dilution.		#For optimal ''[[Streptomyces venezuelae]]'' sporulation, add [[trace elements]] at a 1:500 dilution.

ActinoCommittee at 15:39, 25 June 2019

2019-06-25T15:39:23Z

← Older revision		Revision as of 15:39, 25 June 2019
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	=MYM Medium=		=MYM Medium=

			Maltose-Yeast Extract-Malt Extract (MYM) medium is a rich medium used especially for the growth of ''[[Streptomyces venezuelae]]'', as it sporulates to >90% completion in liquid MYM (Bibb et al 2012). Other <em>Streptomyces</em> sp. may also grow well on this medium.

	~~==For 1 Litre:==~~

			==Preparation==
			Per litre:
	*4g Maltose		*4g Maltose

	*4g Yeast Extract Oxoid		*4g Yeast Extract Oxoid

	*10g Malt Extract		*10g Malt Extract

	*500ml Tap Water		*500ml Tap Water

	*500ml dH2O		*500ml dH2O



	'''Instructions'''		'''Instructions'''
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	#For solid medium add add 2g agar & 100mL liquid MYM to each flask		#For solid medium add add 2g agar & 100mL liquid MYM to each flask
	#Autoclave, expect a precipitate to form afterward.		#Autoclave, expect a precipitate to form afterward.
	#~~To encourage~~ ''[[Streptomyces venezuelae]]'' sporulation, add [[trace elements]] at a 1:500 dilution.		#For optimal ''[[Streptomyces venezuelae]]'' sporulation, add [[trace elements]] at a 1:500 dilution.

			==Uses==
			*routine growth of ''[[Streptomyces venezuelae]]''
			*developmental studies on ''[[Streptomyces venezuelae]]''

			==Notes==
			If using for growth measurements in liquid medium, it is best to allow the precipitate to settle overnight before pipetting the medium into growth vessels - take from the top to avoid the precipitate.

			==References==
			Stuttard C. 1982. Temperate phages of Streptomyces venezuelae: lysogeny and host specificity shown by phages SV1 and SV2. Microbiology 128:115–121.

	~~Note: If using for growth measurements in liquid medium~~, ~~it may be best to allow~~ the ~~precipitate to settle overnight before pipetting medium into growth vessels~~, ~~taking from the top to avoid the precipitate~~.		Bibb MJ, Domonkos A, Chandra G, Buttner MJ. 2012. Expression of the chaplin and rodlin hydrophobic sheath proteins in Streptomyces venezuelae is controlled by σBldN and a cognate anti-sigma factor, RsbN. Mol. Microbiol. 84:1033–1049

Test at 16:24, 6 May 2019

2019-05-06T16:24:35Z

← Older revision		Revision as of 16:24, 6 May 2019
Line 24:		Line 24:
	#For solid medium add add 2g agar & 100mL liquid MYM to each flask		#For solid medium add add 2g agar & 100mL liquid MYM to each flask
	#Autoclave, expect a precipitate to form afterward.		#Autoclave, expect a precipitate to form afterward.
	#To encourage [[''Streptomyces venezuelae'']] sporulation, add [[trace elements]] at a 1:500 dilution.		#To encourage ''[[Streptomyces venezuelae]]'' sporulation, add [[trace elements]] at a 1:500 dilution.

	Note: If using for growth measurements in liquid medium, it may be best to allow the precipitate to settle overnight before pipetting medium into growth vessels, taking from the top to avoid the precipitate.		Note: If using for growth measurements in liquid medium, it may be best to allow the precipitate to settle overnight before pipetting medium into growth vessels, taking from the top to avoid the precipitate.

Test at 16:24, 6 May 2019

2019-05-06T16:24:05Z

← Older revision		Revision as of 16:24, 6 May 2019
Line 24:		Line 24:
	#For solid medium add add 2g agar & 100mL liquid MYM to each flask		#For solid medium add add 2g agar & 100mL liquid MYM to each flask
	#Autoclave, expect a precipitate to form afterward.		#Autoclave, expect a precipitate to form afterward.
	#To encourage "Streptomyces venezuelae" sporulation, add [[trace elements]] at a 1:500 dilution.		#To encourage [[''Streptomyces venezuelae'']] sporulation, add [[trace elements]] at a 1:500 dilution.

	Note: If using for growth measurements in liquid medium, it may be best to allow the precipitate to settle overnight before pipetting medium into growth vessels, taking from the top to avoid the precipitate.		Note: If using for growth measurements in liquid medium, it may be best to allow the precipitate to settle overnight before pipetting medium into growth vessels, taking from the top to avoid the precipitate.