H Otani at 23:40, 24 March 2020

2020-03-24T23:40:27Z

← Older revision		Revision as of 23:40, 24 March 2020
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	[[Meganuclease I-SceI based system for gene deletions]]		[[Meganuclease I-SceI based system for gene deletions]]

			[[Protoplasts Formation, Regeneration and Transformation]]

	[["Quikchange" site-directed mutagenesis]]		[["Quikchange" site-directed mutagenesis]]

MFeeney: Created page with "A number of different methods have been developed for random and directed mutagenesis in actinobacteria (most notably for Streptomyces coelicolor). Many involve an in..."

2019-08-20T16:01:29Z

Created page with "A number of different methods have been developed for random and directed mutagenesis in actinobacteria (most notably for Streptomyces coelicolor). Many involve an in..."

New page

A number of different methods have been developed for random and directed mutagenesis in actinobacteria (most notably for Streptomyces coelicolor). Many involve an initial mutagenesis step performed in Escherichia coli, with subsequent transfer of the mutant allele(s) into the desired strain i.e. by [[protoplast transformation]] or [[intergeneric conjugation into Streptomyces]]. Others, such as CRISPR/Cas- or meganuclease I-SceI- mediated genome editing, can be performed directly in the actinobacterial strain of interest.

Your choice of mutagenesis method will depend largely on your intended use, and on whether you have an appropriate genetic screen/selection that you can use to identify your mutant(s) of interest.

[[CRISPR/Cas9-mediated genome editing]]

[[Chemical mutagenesis]]

[[Depletion strains]]

[[Lambda-red mediated recombination (PCR-targeting system a.k.a. "Redirect")]]

[[Lambda-red mediated recombination using ssDNA]]

[[Meganuclease I-SceI based system for gene deletions]]

[["Quikchange" site-directed mutagenesis]]

[[Random mutagenesis using a mutator strain]]

[[Random mutagenesis using error-prone PCR]]

[[Suicide vectors]]

[[Transposons]]

[[UV mutagenesis]]

Mutagenesis - Revision history

H Otani at 23:40, 24 March 2020

MFeeney: Created page with "A number of different methods have been developed for random and directed mutagenesis in actinobacteria (most notably for Streptomyces coelicolor). Many involve an in..."