Plant-Microbe interactions - Revision history

Jake Newitt at 12:41, 28 March 2022

2022-03-28T12:41:32Z

← Older revision		Revision as of 12:41, 28 March 2022
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	In recent years it has become clear that actinomycete bacteria like <em>Streptomyces</em> species are not simply free-living soil bacteria. They also interact with plant roots and can colonise the rhizosphere and endosphere (inside the roots). For example, they are highly enriched inside the roots of bread wheat plants, which are a staple crop for humans ([https://pubmed.ncbi.nlm.nih.gov/34154664/ Prudence et al, 2021]). This is fascinating because it helps us understand the natural ecology of these bacteria and their natural products - i.e. why they evolved, how they are regulated - and also because they may have uses as plant beneficial bacteria, e.g., by incorporating <em>Streptomyces</em> spores into seed coatings.		In recent years it has become clear that actinomycete bacteria like <em>Streptomyces</em> species are not simply free-living soil bacteria. They also interact with plant roots and can colonise the rhizosphere and endosphere (inside the roots). For example, they are highly enriched inside the roots of bread wheat plants, which are a staple crop for humans ([https://pubmed.ncbi.nlm.nih.gov/34154664/ Prudence et al, 2021]). This is fascinating because it helps us understand the natural ecology of these bacteria and their natural products - i.e. why they evolved, how they are regulated - and also because they may have uses as plant beneficial bacteria, e.g., by incorporating <em>Streptomyces</em> spores into seed coatings.

	~~<h2><strong>Protocols</strong></h2>~~

	<h3><strong>''in vitro'' protocols</strong></h3>
			<h2><strong>''In vitro'' protocols</strong></h2>

	*[[Wheat Take-all bioassays]]		*[[Wheat Take-all bioassays]]

	<h3><strong>''in planta'' protocols</strong></h3>		<h2><strong>''In planta'' protocols</strong></h2>

	<h4><strong>''Arabidopsis'' protocols</strong></h4>		<h3><strong>''Arabidopsis'' protocols</strong></h3>
	*[[Imaging GFP streptomyces on arabidopsis roots\| Imaging GFP Streptomyces on ''Arabidopsis'' roots]]		*[[Imaging GFP streptomyces on arabidopsis roots\| Imaging GFP Streptomyces on ''Arabidopsis'' roots]]

	<h4><strong>''Marchantia'' protocols</strong></h4>		<h3><strong>''Marchantia'' protocols</strong></h3>

	*[[Growing Marchantia from gemma spores \| Growing ''Marchantia'' from gemmae]]		*[[Growing Marchantia from gemma spores \| Growing ''Marchantia'' from gemmae]]

	<h4><strong>Wheat protocols</h4></strong>		<h3><strong>Wheat protocols</h3></strong>

	*[[Isolating Streptomyces from plant roots\| Isolating ''Streptomyces'' from plant roots]]		*[[Isolating Streptomyces from plant roots\| Isolating ''Streptomyces'' from plant roots]]

Jake Newitt at 10:05, 28 March 2022

2022-03-28T10:05:47Z

← Older revision		Revision as of 10:05, 28 March 2022
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	<h3><strong>''in vitro'' protocols</strong></h3>		<h3><strong>''in vitro'' protocols</strong></h3>

			*[[Wheat Take-all bioassays]]

	<h3><strong>''in planta'' protocols</strong></h3>		<h3><strong>''in planta'' protocols</strong></h3>

Jake Newitt at 10:03, 28 March 2022

2022-03-28T10:03:47Z

← Older revision		Revision as of 10:03, 28 March 2022
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	<h3><strong>''in vitro'' protocols</strong></h3>		<h3><strong>''in vitro'' protocols</strong></h3>

			<h3><strong>''in planta'' protocols</strong></h3>

			<h4><strong>''Arabidopsis'' protocols</strong></h4>
			*[[Imaging GFP streptomyces on arabidopsis roots\| Imaging GFP Streptomyces on ''Arabidopsis'' roots]]

	<h4><strong>''Marchantia'' protocols</strong></h4>		<h4><strong>''Marchantia'' protocols</strong></h4>
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	*[[Wheat seed sterilisation]]		*[[Wheat seed sterilisation]]
	*[[Wheat root exudate harvesting\| Harvesting root exudates]]		*[[Wheat root exudate harvesting\| Harvesting root exudates]]

	~~<h3><strong>''in planta'' protocols</strong></h3>~~

	~~<h4><strong>''Arabidopsis'' protocols</strong></h4>~~
	*[[Imaging GFP streptomyces on arabidopsis roots\| Imaging GFP Streptomyces on ''Arabidopsis'' roots]]

	<h2><strong>Further Reading</strong></h2>		<h2><strong>Further Reading</strong></h2>

Jake Newitt at 09:59, 28 March 2022

2022-03-28T09:59:05Z

← Older revision		Revision as of 09:59, 28 March 2022
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	<h2><strong>Protocols</strong></h2>		<h2><strong>Protocols</strong></h2>

	<h3><strong>''Marchantia'' protocols</strong></h3>		<h3><strong>''in vitro'' protocols</strong></h3>

			<h4><strong>''Marchantia'' protocols</strong></h4>

	*[[Growing Marchantia from gemma spores \| Growing ''Marchantia'' from gemmae]]		*[[Growing Marchantia from gemma spores \| Growing ''Marchantia'' from gemmae]]

	<h3><strong>Wheat protocols</h3></strong>		<h4><strong>Wheat protocols</h4></strong>

	*[[Isolating Streptomyces from plant roots\| Isolating ''Streptomyces'' from plant roots]]		*[[Isolating Streptomyces from plant roots\| Isolating ''Streptomyces'' from plant roots]]
	*[[Wheat seed sterilisation]]		*[[Wheat seed sterilisation]]
	*[[Wheat root exudate harvesting\| Harvesting root exudates]]		*[[Wheat root exudate harvesting\| Harvesting root exudates]]

			<h3><strong>''in planta'' protocols</strong></h3>

			<h4><strong>''Arabidopsis'' protocols</strong></h4>
			*[[Imaging GFP streptomyces on arabidopsis roots\| Imaging GFP Streptomyces on ''Arabidopsis'' roots]]

	<h2><strong>Further Reading</strong></h2>		<h2><strong>Further Reading</strong></h2>

Damien Gayrard at 15:29, 2 November 2021

2021-11-02T15:29:41Z

← Older revision		Revision as of 15:29, 2 November 2021
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	<h3><strong>''Marchantia'' protocols</strong></h3>		<h3><strong>''Marchantia'' protocols</strong></h3>

	*[[Growing Marchantia from ~~gemmae~~ \| Growing ''Marchantia'' from gemmae]]		*[[Growing Marchantia from gemma spores \| Growing ''Marchantia'' from gemmae]]

	<h3><strong>Wheat protocols</h3></strong>		<h3><strong>Wheat protocols</h3></strong>

Damien Gayrard at 15:27, 2 November 2021

2021-11-02T15:27:58Z

← Older revision		Revision as of 15:27, 2 November 2021
Line 7:		Line 7:
	<h3><strong>''Marchantia'' protocols</strong></h3>		<h3><strong>''Marchantia'' protocols</strong></h3>

	*[[Growing Marchantia from gemmae \| Growing ''Marchantia'' from gemmae ]]		*[[Growing Marchantia from gemmae \| Growing ''Marchantia'' from gemmae]]

	<h3><strong>Wheat protocols</h3></strong>		<h3><strong>Wheat protocols</h3></strong>

Damien Gayrard at 15:27, 2 November 2021

2021-11-02T15:27:24Z

← Older revision		Revision as of 15:27, 2 November 2021
Line 7:		Line 7:
	<h3><strong>''Marchantia'' protocols</strong></h3>		<h3><strong>''Marchantia'' protocols</strong></h3>

	*[[Growing Marchantia from ~~gemma spores~~\| Growing ''Marchantia'' from ~~gemma spores~~]]		*[[Growing Marchantia from gemmae \| Growing ''Marchantia'' from gemmae ]]

	<h3><strong>Wheat protocols</h3></strong>		<h3><strong>Wheat protocols</h3></strong>

Louisa perry jic at 12:53, 29 October 2021

2021-10-29T12:53:34Z

← Older revision		Revision as of 12:53, 29 October 2021
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	*[[Isolating Streptomyces from plant roots\| Isolating ''Streptomyces'' from plant roots]]		*[[Isolating Streptomyces from plant roots\| Isolating ''Streptomyces'' from plant roots]]
	*[[Wheat seed sterilisation]]		*[[Wheat seed sterilisation]]
	*[[Wheat root exudate harvesting]]		*[[Wheat root exudate harvesting\| Harvesting root exudates]]

	<h2><strong>Further Reading</strong></h2>		<h2><strong>Further Reading</strong></h2>

Louisa perry jic at 12:37, 29 October 2021

2021-10-29T12:37:28Z

← Older revision		Revision as of 12:37, 29 October 2021
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	*[[Isolating Streptomyces from plant roots\| Isolating ''Streptomyces'' from plant roots]]		*[[Isolating Streptomyces from plant roots\| Isolating ''Streptomyces'' from plant roots]]
	*[[Wheat seed sterilisation]]		*[[Wheat seed sterilisation]]
	*[[Harvesting root exudates]]
	*[[Wheat root exudate harvesting]]		*[[Wheat root exudate harvesting]]

Louisa perry jic at 12:22, 29 October 2021

2021-10-29T12:22:50Z

← Older revision		Revision as of 12:22, 29 October 2021
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	*[[Wheat seed sterilisation]]		*[[Wheat seed sterilisation]]
	*[[Harvesting root exudates]]		*[[Harvesting root exudates]]
			*[[Wheat root exudate harvesting]]

	<h2><strong>Further Reading</strong></h2>		<h2><strong>Further Reading</strong></h2>