SFM - Revision history

Sam Prudence: /* Mannitol Soya Flour (MS) a.k.a. Soya Flour Mannitol (SFM) Medium */

2019-09-26T15:23:17Z

Mannitol Soya Flour (MS) a.k.a. Soya Flour Mannitol (SFM) Medium

← Older revision		Revision as of 15:23, 26 September 2019
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	<sup>*</sup>SFM became routinely used as an acronym for this media to avoid confusion with the plant growth medium Murashige & Skoog medium in the John Innes Media kitchen.		<sup>*</sup>SFM became routinely used as an acronym for this media to avoid confusion with the plant growth medium Murashige & Skoog medium in the John Innes Media kitchen.





	==Preparation==		==Preparation==

Sam Prudence: /* Mannitol Soya Flour (MS) a.k.a. Soya Flour Mannitol (SFM) Medium */

2019-09-26T15:22:56Z

Mannitol Soya Flour (MS) a.k.a. Soya Flour Mannitol (SFM) Medium

← Older revision		Revision as of 15:22, 26 September 2019
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	=Mannitol Soya Flour (MS) a.k.a. Soya Flour Mannitol (SFM) Medium=		=Mannitol Soya Flour (MS) a.k.a. Soya Flour Mannitol (SFM) Medium=
			[[File:Streptomyces.png \| ''Streptomyces coelicolor'' growing on SFM agar \| thumb]]

	Mannitol Soya Flour (MS) medium or as it is often called Soya Flour Mannitol (SFM)<sup>*</sup> medium, is a complex medium used for the routine cultivation of ''Streptomyces'' sp. Most species will sporulate well on this medium.		Mannitol Soya Flour (MS) medium or as it is often called Soya Flour Mannitol (SFM)<sup>*</sup> medium, is a complex medium used for the routine cultivation of ''Streptomyces'' sp. Most species will sporulate well on this medium.
	~~[[File:Streptomyces.png \| ''Streptomyces coelicolor'' growing on SFM agar \| thumb]]~~
	<sup>*</sup>SFM became routinely used as an acronym for this media to avoid confusion with the plant growth medium Murashige & Skoog medium in the John Innes Media kitchen.		<sup>*</sup>SFM became routinely used as an acronym for this media to avoid confusion with the plant growth medium Murashige & Skoog medium in the John Innes Media kitchen.

Sam Prudence at 15:22, 26 September 2019

2019-09-26T15:22:35Z

← Older revision		Revision as of 15:22, 26 September 2019
Line 2:		Line 2:

	Mannitol Soya Flour (MS) medium or as it is often called Soya Flour Mannitol (SFM)<sup>*</sup> medium, is a complex medium used for the routine cultivation of ''Streptomyces'' sp. Most species will sporulate well on this medium.		Mannitol Soya Flour (MS) medium or as it is often called Soya Flour Mannitol (SFM)<sup>*</sup> medium, is a complex medium used for the routine cultivation of ''Streptomyces'' sp. Most species will sporulate well on this medium.
			[[File:Streptomyces.png \| ''Streptomyces coelicolor'' growing on SFM agar \| thumb]]
	<sup>*</sup>SFM became routinely used as an acronym for this media to avoid confusion with the plant growth medium Murashige & Skoog medium in the John Innes Media kitchen.		<sup>*</sup>SFM became routinely used as an acronym for this media to avoid confusion with the plant growth medium Murashige & Skoog medium in the John Innes Media kitchen.

MFeeney at 13:13, 31 July 2019

2019-07-31T13:13:24Z

← Older revision		Revision as of 13:13, 31 July 2019
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	=Mannitol Soya Flour (MS) a.k.a. Soya Flour Mannitol (SFM) Medium=		=Mannitol Soya Flour (MS) a.k.a. Soya Flour Mannitol (SFM) Medium=

	Mannitol Soya Flour (MS) medium or as it is often called Soya Flour Mannitol (SFM)* medium, is a complex medium used for the routine cultivation of ''Streptomyces'' sp. Most species will sporulate well on this medium.		Mannitol Soya Flour (MS) medium or as it is often called Soya Flour Mannitol (SFM)<sup>*</sup> medium, is a complex medium used for the routine cultivation of ''Streptomyces'' sp. Most species will sporulate well on this medium.

	*SFM became routinely used as an acronym for this media to avoid confusion with the plant growth medium Murashige & Skoog medium in the John Innes Media kitchen.		<sup>*</sup>SFM became routinely used as an acronym for this media to avoid confusion with the plant growth medium Murashige & Skoog medium in the John Innes Media kitchen.

	==Preparation==		==Preparation==

MFeeney at 13:12, 31 July 2019

2019-07-31T13:12:51Z

← Older revision		Revision as of 13:12, 31 July 2019
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	=Mannitol Soya Flour (MS) a.k.a. Soya Flour Mannitol (SFM) Medium=		=Mannitol Soya Flour (MS) a.k.a. Soya Flour Mannitol (SFM) Medium=

	Soya Flour ~~Mannitol~~ (~~SFM~~) medium~~, also~~ called ~~Mannitol~~ Soya Flour (MS) medium, is a complex medium used for the routine cultivation of ''Streptomyces'' sp. Most will sporulate well on this medium.		Mannitol Soya Flour (MS) medium or as it is often called Soya Flour Mannitol (SFM)* medium, is a complex medium used for the routine cultivation of ''Streptomyces'' sp. Most species will sporulate well on this medium.

			*SFM became routinely used as an acronym for this media to avoid confusion with the plant growth medium Murashige & Skoog medium in the John Innes Media kitchen.

	==Preparation==		==Preparation==
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	Soya flour is available from many health food shops and supermarkets (it is not necessary to purchase the more expensive material from a laboratory supplier).		Soya flour is available from many health food shops and supermarkets (it is not necessary to purchase the more expensive material from a laboratory supplier).

	Liquid MS~~/SFM~~ medium may be made by omitting the agar.		Liquid MS medium may be made by omitting the agar.

	Kieser et al (2000) recommend autoclaving MS~~/SFM~~ twice. Though this is not strictly necessary, the second autoclave cycle can help to break down some of the complex carbohydrates present in the soy flour, making them more readily available to the Streptomyces. Thus, you may observe improved growth and/or development when growing your strains on 2x autoclaved MS~~/SFM~~.		Kieser et al (2000) recommend autoclaving MS twice. Though this is not strictly necessary, the second autoclave cycle can help to break down some of the complex carbohydrates present in the soy flour, making them more readily available to the Streptomyces. Thus, you may observe improved growth and/or development when growing your strains on 2x autoclaved MS.

	''[[Streptomyces venezuelae]]'' and ''[[Streptomyces clavuligerus]]'' do not sporulate well on MS~~/SFM~~ medium.		''[[Streptomyces venezuelae]]'' and ''[[Streptomyces clavuligerus]]'' do not sporulate well on MS medium.

	MS~~/SFM~~ may also be used for bioassays, especially if supplemented with e.g. [[N-acetylglucosamine]] or [[sodium butyrate]] to elicit antibiotic production.		MS may also be used for bioassays, especially if supplemented with e.g. [[N-acetylglucosamine]] or [[sodium butyrate]] to elicit antibiotic production.

	==References==		==References==

MFeeney at 10:01, 30 July 2019

2019-07-30T10:01:32Z

← Older revision		Revision as of 10:01, 30 July 2019
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	~~{{DISPLAYTITLE: MS or SFM}}~~

	=Mannitol Soya Flour (MS) a.k.a. Soya Flour Mannitol (SFM) Medium=		=Mannitol Soya Flour (MS) a.k.a. Soya Flour Mannitol (SFM) Medium=

MFeeney at 09:59, 30 July 2019

2019-07-30T09:59:00Z

← Older revision		Revision as of 09:59, 30 July 2019
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	[DISPLAYTITLE: MS or SFM]		{{DISPLAYTITLE: MS or SFM}}

	=Mannitol Soya Flour (MS) a.k.a. Soya Flour Mannitol (SFM) Medium=		=Mannitol Soya Flour (MS) a.k.a. Soya Flour Mannitol (SFM) Medium=

MFeeney at 09:58, 30 July 2019

2019-07-30T09:58:04Z

← Older revision		Revision as of 09:58, 30 July 2019
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	{DISPLAYTITLE: MS or SFM}		[DISPLAYTITLE: MS or SFM]

	=Mannitol Soya Flour (MS) a.k.a. Soya Flour Mannitol (SFM) Medium=		=Mannitol Soya Flour (MS) a.k.a. Soya Flour Mannitol (SFM) Medium=

MFeeney at 09:57, 30 July 2019

2019-07-30T09:57:34Z

← Older revision		Revision as of 09:57, 30 July 2019
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	~~=Soy Flour Mannitol Medium=~~		{DISPLAYTITLE: MS or SFM}

	~~Soy~~ Flour Mannitol (SFM) medium, also called Mannitol ~~Soy~~ Flour (MS) medium, is a complex medium used for the routine cultivation of ''Streptomyces'' sp. Most will sporulate well on this medium.		=Mannitol Soya Flour (MS) a.k.a. Soya Flour Mannitol (SFM) Medium=

			Soya Flour Mannitol (SFM) medium, also called Mannitol Soya Flour (MS) medium, is a complex medium used for the routine cultivation of ''Streptomyces'' sp. Most will sporulate well on this medium.

	==Preparation==		==Preparation==
	Per Litre:		Per Litre:
	*20 g Mannitol		*20 g Mannitol
	*20 g ~~Soy~~ Flour		*20 g Soya Flour
	*20 g Agar		*20 g Agar
	*1 L tap water		*1 L tap water
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	'''Instructions'''		'''Instructions'''
	#Dissolve 20g mannitol in 1L tap water		#Dissolve 20g mannitol in 1L tap water
	#Add 2g ~~soy~~ flour and 2g agar to each 250mL flask		#Add 2g soya flour and 2g agar to each 250mL flask
	#Add 100mL mannitol solution to each flask		#Add 100mL mannitol solution to each flask
	#Autoclave		#Autoclave
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	Soya flour is available from many health food shops and supermarkets (it is not necessary to purchase the more expensive material from a laboratory supplier).		Soya flour is available from many health food shops and supermarkets (it is not necessary to purchase the more expensive material from a laboratory supplier).

	Liquid SFM medium may be made by omitting the agar.		Liquid MS/SFM medium may be made by omitting the agar.

	Kieser et al (2000) recommend autoclaving SFM twice. Though this is not strictly necessary, the second autoclave cycle can help to break down some of the complex carbohydrates present in the soy flour, making them more readily available to the Streptomyces. Thus, you may observe improved growth and/or development when growing your strains on 2x autoclaved SFM.		Kieser et al (2000) recommend autoclaving MS/SFM twice. Though this is not strictly necessary, the second autoclave cycle can help to break down some of the complex carbohydrates present in the soy flour, making them more readily available to the Streptomyces. Thus, you may observe improved growth and/or development when growing your strains on 2x autoclaved MS/SFM.

	''[[Streptomyces venezuelae]]'' and ''[[Streptomyces clavuligerus]]'' do not sporulate well on SFM medium.		''[[Streptomyces venezuelae]]'' and ''[[Streptomyces clavuligerus]]'' do not sporulate well on MS/SFM medium.

	SFM may also be used for bioassays, especially if supplemented with e.g. [[N-acetylglucosamine]] or [[sodium butyrate]] to elicit antibiotic production.		MS/SFM may also be used for bioassays, especially if supplemented with e.g. [[N-acetylglucosamine]] or [[sodium butyrate]] to elicit antibiotic production.

	==References==		==References==

LisAlgora at 16:55, 26 July 2019

2019-07-26T16:55:52Z

← Older revision		Revision as of 16:55, 26 July 2019
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	==Preparation==		==Preparation==
	Per Litre:		Per Litre:
	*~~20g~~ Mannitol		*20 g Mannitol
	*~~20g~~ Soy Flour		*20 g Soy Flour
	*~~20g~~ Agar		*20 g Agar
	*~~1L with~~ tap water		*1 L tap water

	'''Instructions'''		'''Instructions'''