MFeeney: /* Preparation */

2022-07-19T10:24:38Z

Preparation

← Older revision		Revision as of 10:24, 19 July 2022
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	*81.9 mL PEG 6000 (BDH 6.1% w/v in distilled water)		*81.9 mL PEG 6000 (BDH 6.1% w/v in distilled water)
	*2.5 mL Mg<sub>2</sub>SO<sub>4</sub>.7H<sub>2</sub>O - 5mM final		*2.5 mL Mg<sub>2</sub>SO<sub>4</sub>.7H<sub>2</sub>O - 5mM final
	*10 mL TES ~~bugger~~ (0.25M, pH 7.2) - 25mM final		*10 mL TES buffer (0.25M, pH 7.2) - 25mM final
	*1 mL NaH<sub>2</sub>PO<sub>4</sub> + K<sub>2</sub>HPO<sub>4</sub> (50mM each) - 1mM final		*1 mL NaH<sub>2</sub>PO<sub>4</sub> + K<sub>2</sub>HPO<sub>4</sub> (50mM each) - 1mM final
	*2 mL glucose (50% w/v)		*2 mL glucose (50% w/v)

Tommclean: Created page with "=SMMS (Supplemented liquid minimal media, solid)= SMM is a solid supplemented minimal medium suitable for growth of many ''Streptomyces'' sp. ==Preparation== Per litre: *81.9..."

2019-08-01T15:57:57Z

Created page with "=SMMS (Supplemented liquid minimal media, solid)= SMM is a solid supplemented minimal medium suitable for growth of many ''Streptomyces'' sp. ==Preparation== Per litre: *81.9..."

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=SMMS (Supplemented liquid minimal media, solid)=
SMM is a solid supplemented minimal medium suitable for growth of many ''Streptomyces'' sp.

==Preparation==
Per litre:
*81.9 mL PEG 6000 (BDH 6.1% w/v in distilled water)
*2.5 mL Mg2SO4.7H2O - 5mM final
*10 mL TES bugger (0.25M, pH 7.2) - 25mM final
*1 mL NaH2PO4 + K2HPO4 (50mM each) - 1mM final
*2 mL glucose (50% w/v)
*1 mL Antifoam 289 (Sigma A 5551)(1% w/v)
*0.1 mL Trace element solution
*1 mL Difco Casaminoacids (20% w/v)
*100 mL Total
*25 ml/L Glycine (0.5% final)

Trace element solution:
0.1 g/L of the following:
*ZnSO4.7H2O
*FeSO4.7H2O
*MnCl2.4H2O
*CaCl2.6H2O
*NaCl
Make fresh every 2-4 weeks and filter-sterilise. Store at 4º

'''Instructions'''
#With the exception of the trace element solution each solution must be made up and autoclaved separately
#The solutions should be mixed in the given order and then made up to 1L with sterile distilled water
#Addition of 10 g/L agar

==References==
Strauch E, Takano E, Baylts H, Bibb M. The stringent response in ''Streptomyces coelicolor'' A3(2). ''Molecular Microbiology''. 1991;5(2):289-298.

SMMS - Revision history

MFeeney: /* Preparation */

Tommclean: Created page with "=SMMS (Supplemented liquid minimal media, solid)= SMM is a solid supplemented minimal medium suitable for growth of many ''Streptomyces'' sp. ==Preparation== Per litre: *81.9..."