Jacob Hamilton at 15:56, 1 August 2019

2019-08-01T15:56:45Z

← Older revision		Revision as of 15:56, 1 August 2019
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	==References==		==References==
	~~DOI:~~ [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4421039/ Imai, Y., Sato, S., Tanaka, Y., Ochi, K. and Hosaka, T. (2015). Lincomycin at Subinhibitory Concentrations Potentiates Secondary Metabolite Production by Streptomyces spp. Applied and Environmental Microbiology, 81(11), pp.3869-3879.]		[https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4421039/ Imai, Y., Sato, S., Tanaka, Y., Ochi, K. and Hosaka, T. (2015). Lincomycin at Subinhibitory Concentrations Potentiates Secondary Metabolite Production by Streptomyces spp. Applied and Environmental Microbiology, 81(11), pp.3869-3879.]

Jacob Hamilton: Created page with "=Salt Peptone Yeast Agar (SPY)= Salt Peptone Yeast agar is a metal-rich media used to stimulate the activation of cryptic biosynthetic gene clusters as part of antibiotic dis..."

2019-08-01T15:56:18Z

Created page with "=Salt Peptone Yeast Agar (SPY)= Salt Peptone Yeast agar is a metal-rich media used to stimulate the activation of cryptic biosynthetic gene clusters as part of antibiotic dis..."

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=Salt Peptone Yeast Agar (SPY)=

Salt Peptone Yeast agar is a metal-rich media used to stimulate the activation of cryptic biosynthetic gene clusters as part of antibiotic discovery.

==Preparation==
Per Litre:
*30g Soluable Starch
*15g pancreatic digest of casein
*15g peptic digest of animal tissue
*2g Yeast Extract
*10g MgSO4
*50mg CaCl2
*30mg ZnSO4
*25mg FeSO4
*16mg CuSO4
*12mg MnSO4
*20g agar
*NaOH (to adust pH)
*1L dH2O

'''Instructions'''
#On a heated stiring block, warm the water and add the pancreatice digest of casin, the peptic digest of animal tissue, the yeast extract and the trace elements.
#Add 2g of agar into each of 10 250mL conical flasks.
#Use NaOH to adjust the pH of the solution to pH 7.2.
#Dissolve the soluable starch in the solution. It can take a while for the starch to dissolve.
#Pour 100mL of solution into each flask.
#Autoclave

==Uses==

==Notes==
*To dissolve the soluable starch, it is often required to make the media on a hot plate. Do not allow the solution to boil.
*To add the trace elements, it may be easier to make concentrated stock solution which is then added to the media prior to pH adjustment.

==References==
DOI: [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4421039/ Imai, Y., Sato, S., Tanaka, Y., Ochi, K. and Hosaka, T. (2015). Lincomycin at Subinhibitory Concentrations Potentiates Secondary Metabolite Production by Streptomyces spp. Applied and Environmental Microbiology, 81(11), pp.3869-3879.]

SPY - Revision history

Jacob Hamilton at 15:56, 1 August 2019

Jacob Hamilton: Created page with "=Salt Peptone Yeast Agar (SPY)= Salt Peptone Yeast agar is a metal-rich media used to stimulate the activation of cryptic biosynthetic gene clusters as part of antibiotic dis..."