https://actinobase.org/index.php?action=history&feed=atom&title=Streptomyces_Bacteriophages
Streptomyces Bacteriophages - Revision history
2026-10-17T21:06:45Z
Revision history for this page on the wiki
MediaWiki 1.39.3
https://actinobase.org/index.php?title=Streptomyces_Bacteriophages&diff=2036&oldid=prev
MFeeney: /* Protocols */
2022-03-20T21:45:21Z
<p><span dir="auto"><span class="autocomment">Protocols</span></span></p>
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 21:45, 20 March 2022</td>
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<tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">[[SV1 transduction]]</ins></div></td></tr>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>==Links==</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>==Links==</div></td></tr>
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MFeeney
https://actinobase.org/index.php?title=Streptomyces_Bacteriophages&diff=2034&oldid=prev
Jake Newitt at 16:44, 3 March 2022
2022-03-03T16:44:13Z
<p></p>
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<tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">==Links==</ins></div></td></tr>
<tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">[[Site-specific phage recombinase | Site-specific phage recombinase targets]]</ins></div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">[https://phagesdb.org/ The Actinobacteriophage Database]</ins></div></td></tr>
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Jake Newitt
https://actinobase.org/index.php?title=Streptomyces_Bacteriophages&diff=1687&oldid=prev
MFeeney at 08:48, 5 May 2020
2020-05-05T08:48:35Z
<p></p>
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 08:48, 5 May 2020</td>
</tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l9">Line 9:</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>[[File:PhiC31 plaque assay.jpg|thumb|'''Example of a ''Streptomyces'' bacteriophage.''' A plaque assay was performed using decimal serial dilutions of ΦC31 and a ''pglW<sup>-</sup>'' strain of ''S. coelicolor''.]]</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>[[File:PhiC31 plaque assay.jpg|thumb|'''Example of a ''Streptomyces'' bacteriophage.''' A plaque assay was performed using decimal serial dilutions of ΦC31 and a ''pglW<sup>-</sup>'' strain of ''S. coelicolor''.]]</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>''Streptomyces'' phages are readily isolated from a range of soil and environmental samples on soft agar overnight (see protocol for [[Isolation of phages from environmental samples]]), with isolation of novel phage from single phages easily achieved. Success can be increased through enrichment procedures (see protocol <del style="font-weight: bold; text-decoration: none;">'</del>Isolation of phages from environmental samples<del style="font-weight: bold; text-decoration: none;">'</del>), with the isolation of lytic and temperate phages being relatively straightforward. </div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>''Streptomyces'' phages are readily isolated from a range of soil and environmental samples on soft agar overnight (see protocol for [[Isolation of phages from environmental samples]]), with isolation of novel phage from single phages easily achieved. Success can be increased through enrichment procedures (see protocol <ins style="font-weight: bold; text-decoration: none;">[[</ins>Isolation of phages from environmental samples<ins style="font-weight: bold; text-decoration: none;">]]</ins>), with the isolation of lytic and temperate phages being relatively straightforward. </div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>Phages can also readily be induced from lysogens (prophages) using the standard methods of UV-induction, mitomycin C-induction and from spontaneous induction events following growth in liquid medium, which provides free phage for plaque formation on suitable indicator strains (not always straightforward as endogenous phage often have narrow host ranges and the original strain is likely immune to a phage it carries). </div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>Phages can also readily be induced from lysogens (prophages) using the standard methods of UV-induction, mitomycin C-induction and from spontaneous induction events following growth in liquid medium, which provides free phage for plaque formation on suitable indicator strains (not always straightforward as endogenous phage often have narrow host ranges and the original strain is likely immune to a phage it carries). </div></td></tr>
</table>
MFeeney
https://actinobase.org/index.php?title=Streptomyces_Bacteriophages&diff=1686&oldid=prev
MFeeney at 08:47, 5 May 2020
2020-05-05T08:47:52Z
<p></p>
<table style="background-color: #fff; color: #202122;" data-mw="interface">
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 08:47, 5 May 2020</td>
</tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l7">Line 7:</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>Bacteriophages (or phages) are amongst the most abundant biological entities on the planet and exhibit enormous diversity<sup>[1]</sup>. There has been a huge amount of interest in ''Streptomyces'' phages over many years as they have been a source of many valuable tools for molecular biology and genetic manipulation. These include the widely used integrating vector systems<sup>[2]</sup> based on phage integrases, such as [[pSET152]], [[pMS82]] and their derivatives, along with newer integrase systems<sup>[3–5]</sup>, mutagenesis<sup>[6]</sup> and the older, seldom-used nowadays, phage cloning vectors<sup>[7,8]</sup>. ''Streptomyces'' bacteriophages have also been extensively studied from the perspective of their own biology and diversity<sup>[1,9,10]</sup>, and also in ''Streptomyces'' from the perspective of bacteriophage resistance<sup>[11,12]</sup>.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>Bacteriophages (or phages) are amongst the most abundant biological entities on the planet and exhibit enormous diversity<sup>[1]</sup>. There has been a huge amount of interest in ''Streptomyces'' phages over many years as they have been a source of many valuable tools for molecular biology and genetic manipulation. These include the widely used integrating vector systems<sup>[2]</sup> based on phage integrases, such as [[pSET152]], [[pMS82]] and their derivatives, along with newer integrase systems<sup>[3–5]</sup>, mutagenesis<sup>[6]</sup> and the older, seldom-used nowadays, phage cloning vectors<sup>[7,8]</sup>. ''Streptomyces'' bacteriophages have also been extensively studied from the perspective of their own biology and diversity<sup>[1,9,10]</sup>, and also in ''Streptomyces'' from the perspective of bacteriophage resistance<sup>[11,12]</sup>.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>[[File:PhiC31 plaque assay.jpg|thumb|'''Example of a ''Streptomyces'' bacteriophage.''' A plaque assay was performed using decimal serial dilutions of ΦC31 and a''pglW<sup>-</sup>'' strain of ''S. coelicolor''.]</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>[[File:PhiC31 plaque assay.jpg|thumb|'''Example of a ''Streptomyces'' bacteriophage.''' A plaque assay was performed using decimal serial dilutions of ΦC31 and a ''pglW<sup>-</sup>'' strain of ''S. coelicolor''.<ins style="font-weight: bold; text-decoration: none;">]</ins>]</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>''Streptomyces'' phages are readily isolated from a range of soil and environmental samples on soft agar overnight (see protocol for [[Isolation of phages from environmental samples]]), with isolation of novel phage from single phages easily achieved. Success can be increased through enrichment procedures (see protocol 'Isolation of phages from environmental samples'), with the isolation of lytic and temperate phages being relatively straightforward. </div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>''Streptomyces'' phages are readily isolated from a range of soil and environmental samples on soft agar overnight (see protocol for [[Isolation of phages from environmental samples]]), with isolation of novel phage from single phages easily achieved. Success can be increased through enrichment procedures (see protocol 'Isolation of phages from environmental samples'), with the isolation of lytic and temperate phages being relatively straightforward. </div></td></tr>
</table>
MFeeney
https://actinobase.org/index.php?title=Streptomyces_Bacteriophages&diff=1685&oldid=prev
MFeeney at 08:47, 5 May 2020
2020-05-05T08:47:31Z
<p></p>
<table style="background-color: #fff; color: #202122;" data-mw="interface">
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<col class="diff-content" />
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td>
<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 08:47, 5 May 2020</td>
</tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l7">Line 7:</td>
<td colspan="2" class="diff-lineno">Line 7:</td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>Bacteriophages (or phages) are amongst the most abundant biological entities on the planet and exhibit enormous diversity<sup>[1]</sup>. There has been a huge amount of interest in ''Streptomyces'' phages over many years as they have been a source of many valuable tools for molecular biology and genetic manipulation. These include the widely used integrating vector systems<sup>[2]</sup> based on phage integrases, such as [[pSET152]], [[pMS82]] and their derivatives, along with newer integrase systems<sup>[3–5]</sup>, mutagenesis<sup>[6]</sup> and the older, seldom-used nowadays, phage cloning vectors<sup>[7,8]</sup>. ''Streptomyces'' bacteriophages have also been extensively studied from the perspective of their own biology and diversity<sup>[1,9,10]</sup>, and also in ''Streptomyces'' from the perspective of bacteriophage resistance<sup>[11,12]</sup>.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>Bacteriophages (or phages) are amongst the most abundant biological entities on the planet and exhibit enormous diversity<sup>[1]</sup>. There has been a huge amount of interest in ''Streptomyces'' phages over many years as they have been a source of many valuable tools for molecular biology and genetic manipulation. These include the widely used integrating vector systems<sup>[2]</sup> based on phage integrases, such as [[pSET152]], [[pMS82]] and their derivatives, along with newer integrase systems<sup>[3–5]</sup>, mutagenesis<sup>[6]</sup> and the older, seldom-used nowadays, phage cloning vectors<sup>[7,8]</sup>. ''Streptomyces'' bacteriophages have also been extensively studied from the perspective of their own biology and diversity<sup>[1,9,10]</sup>, and also in ''Streptomyces'' from the perspective of bacteriophage resistance<sup>[11,12]</sup>.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>[[File:PhiC31 plaque assay.jpg|thumb|<del style="font-weight: bold; text-decoration: none;">ΦC31 </del>plaque assay<del style="font-weight: bold; text-decoration: none;">]</del>]</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>[[File:PhiC31 plaque assay.jpg|thumb|<ins style="font-weight: bold; text-decoration: none;">'''Example of a ''Streptomyces'' bacteriophage.''' A </ins>plaque assay <ins style="font-weight: bold; text-decoration: none;">was performed using decimal serial dilutions of ΦC31 and a''pglW<sup>-</sup>'' strain of ''S. coelicolor''.</ins>]</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>''Streptomyces'' phages are readily isolated from a range of soil and environmental samples on soft agar overnight (see protocol for [[Isolation of phages from environmental samples]]), with isolation of novel phage from single phages easily achieved. Success can be increased through enrichment procedures (see protocol 'Isolation of phages from environmental samples'), with the isolation of lytic and temperate phages being relatively straightforward. </div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>''Streptomyces'' phages are readily isolated from a range of soil and environmental samples on soft agar overnight (see protocol for [[Isolation of phages from environmental samples]]), with isolation of novel phage from single phages easily achieved. Success can be increased through enrichment procedures (see protocol 'Isolation of phages from environmental samples'), with the isolation of lytic and temperate phages being relatively straightforward. </div></td></tr>
</table>
MFeeney
https://actinobase.org/index.php?title=Streptomyces_Bacteriophages&diff=1684&oldid=prev
MFeeney at 08:39, 5 May 2020
2020-05-05T08:39:47Z
<p></p>
<table style="background-color: #fff; color: #202122;" data-mw="interface">
<col class="diff-marker" />
<col class="diff-content" />
<col class="diff-marker" />
<col class="diff-content" />
<tr class="diff-title" lang="en-GB">
<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td>
<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 08:39, 5 May 2020</td>
</tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l6">Line 6:</td>
<td colspan="2" class="diff-lineno">Line 6:</td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>Bacteriophages (or phages) are amongst the most abundant biological entities on the planet and exhibit enormous diversity<sup>[1]</sup>. There has been a huge amount of interest in ''Streptomyces'' phages over many years as they have been a source of many valuable tools for molecular biology and genetic manipulation. These include the widely used integrating vector systems<sup>[2]</sup> based on phage integrases, such as [[pSET152]], [[pMS82]] and their derivatives, along with newer integrase systems<sup>[3–5]</sup>, mutagenesis<sup>[6]</sup> and the older, seldom-used nowadays, phage cloning vectors<sup>[7,8]</sup>. ''Streptomyces'' bacteriophages have also been extensively studied from the perspective of their own biology and diversity<sup>[1,9,10]</sup>, and also in ''Streptomyces'' from the perspective of bacteriophage resistance<sup>[11,12]</sup>.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>Bacteriophages (or phages) are amongst the most abundant biological entities on the planet and exhibit enormous diversity<sup>[1]</sup>. There has been a huge amount of interest in ''Streptomyces'' phages over many years as they have been a source of many valuable tools for molecular biology and genetic manipulation. These include the widely used integrating vector systems<sup>[2]</sup> based on phage integrases, such as [[pSET152]], [[pMS82]] and their derivatives, along with newer integrase systems<sup>[3–5]</sup>, mutagenesis<sup>[6]</sup> and the older, seldom-used nowadays, phage cloning vectors<sup>[7,8]</sup>. ''Streptomyces'' bacteriophages have also been extensively studied from the perspective of their own biology and diversity<sup>[1,9,10]</sup>, and also in ''Streptomyces'' from the perspective of bacteriophage resistance<sup>[11,12]</sup>.</div></td></tr>
<tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">[[File:PhiC31 plaque assay.jpg|thumb|ΦC31 plaque assay]]</ins></div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>''Streptomyces'' phages are readily isolated from a range of soil and environmental samples on soft agar overnight (see protocol for [[Isolation of phages from environmental samples]]), with isolation of novel phage from single phages easily achieved. Success can be increased through enrichment procedures (see protocol 'Isolation of phages from environmental samples'), with the isolation of lytic and temperate phages being relatively straightforward. </div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>''Streptomyces'' phages are readily isolated from a range of soil and environmental samples on soft agar overnight (see protocol for [[Isolation of phages from environmental samples]]), with isolation of novel phage from single phages easily achieved. Success can be increased through enrichment procedures (see protocol 'Isolation of phages from environmental samples'), with the isolation of lytic and temperate phages being relatively straightforward. </div></td></tr>
<tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l13">Line 13:</td>
<td colspan="2" class="diff-lineno">Line 15:</td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>The model Streptomyces phage (our equivalent of the model coliphage, λ) is the temperate phage [[ΦC31]], with clear plaque (lytic) mutants (cΔ25) available and often used in the laboratory. The R4-like group of phages appear to be the most abundant Streptomyces phages globally<sup>[1]</sup>. Beyond the Streptomycetes there have been limited investigations of phage within the Actinobacteria - the exception being the extensive work on Mycobacteriophages<sup>[10,13,14]</sup> and Corynephages<sup>[15]</sup>.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>The model Streptomyces phage (our equivalent of the model coliphage, λ) is the temperate phage [[ΦC31]], with clear plaque (lytic) mutants (cΔ25) available and often used in the laboratory. The R4-like group of phages appear to be the most abundant Streptomyces phages globally<sup>[1]</sup>. Beyond the Streptomycetes there have been limited investigations of phage within the Actinobacteria - the exception being the extensive work on Mycobacteriophages<sup>[10,13,14]</sup> and Corynephages<sup>[15]</sup>.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">[[File:PhiC31 plaque assay.jpg|thumb|ΦC31 plaque assay]]</del></div></td><td colspan="2" class="diff-side-added"></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>==Protocols==</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>==Protocols==</div></td></tr>
</table>
MFeeney
https://actinobase.org/index.php?title=Streptomyces_Bacteriophages&diff=1683&oldid=prev
MFeeney at 08:37, 5 May 2020
2020-05-05T08:37:58Z
<p></p>
<table style="background-color: #fff; color: #202122;" data-mw="interface">
<col class="diff-marker" />
<col class="diff-content" />
<col class="diff-marker" />
<col class="diff-content" />
<tr class="diff-title" lang="en-GB">
<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td>
<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 08:37, 5 May 2020</td>
</tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l13">Line 13:</td>
<td colspan="2" class="diff-lineno">Line 13:</td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>The model Streptomyces phage (our equivalent of the model coliphage, λ) is the temperate phage [[ΦC31]], with clear plaque (lytic) mutants (cΔ25) available and often used in the laboratory. The R4-like group of phages appear to be the most abundant Streptomyces phages globally<sup>[1]</sup>. Beyond the Streptomycetes there have been limited investigations of phage within the Actinobacteria - the exception being the extensive work on Mycobacteriophages<sup>[10,13,14]</sup> and Corynephages<sup>[15]</sup>.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>The model Streptomyces phage (our equivalent of the model coliphage, λ) is the temperate phage [[ΦC31]], with clear plaque (lytic) mutants (cΔ25) available and often used in the laboratory. The R4-like group of phages appear to be the most abundant Streptomyces phages globally<sup>[1]</sup>. Beyond the Streptomycetes there have been limited investigations of phage within the Actinobacteria - the exception being the extensive work on Mycobacteriophages<sup>[10,13,14]</sup> and Corynephages<sup>[15]</sup>.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>[[File:PhiC31 plaque assay.jpg|ΦC31 plaque assay]]</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>[[File:PhiC31 plaque assay.jpg<ins style="font-weight: bold; text-decoration: none;">|thumb</ins>|ΦC31 plaque assay]]</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>==Protocols==</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>==Protocols==</div></td></tr>
</table>
MFeeney
https://actinobase.org/index.php?title=Streptomyces_Bacteriophages&diff=1682&oldid=prev
MFeeney at 08:36, 5 May 2020
2020-05-05T08:36:06Z
<p></p>
<table style="background-color: #fff; color: #202122;" data-mw="interface">
<col class="diff-marker" />
<col class="diff-content" />
<col class="diff-marker" />
<col class="diff-content" />
<tr class="diff-title" lang="en-GB">
<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td>
<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 08:36, 5 May 2020</td>
</tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l13">Line 13:</td>
<td colspan="2" class="diff-lineno">Line 13:</td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>The model Streptomyces phage (our equivalent of the model coliphage, λ) is the temperate phage [[ΦC31]], with clear plaque (lytic) mutants (cΔ25) available and often used in the laboratory. The R4-like group of phages appear to be the most abundant Streptomyces phages globally<sup>[1]</sup>. Beyond the Streptomycetes there have been limited investigations of phage within the Actinobacteria - the exception being the extensive work on Mycobacteriophages<sup>[10,13,14]</sup> and Corynephages<sup>[15]</sup>.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>The model Streptomyces phage (our equivalent of the model coliphage, λ) is the temperate phage [[ΦC31]], with clear plaque (lytic) mutants (cΔ25) available and often used in the laboratory. The R4-like group of phages appear to be the most abundant Streptomyces phages globally<sup>[1]</sup>. Beyond the Streptomycetes there have been limited investigations of phage within the Actinobacteria - the exception being the extensive work on Mycobacteriophages<sup>[10,13,14]</sup> and Corynephages<sup>[15]</sup>.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">[[File:PhiC31 plaque assay.jpg|ΦC31 plaque assay]]</ins></div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>==Protocols==</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>==Protocols==</div></td></tr>
</table>
MFeeney
https://actinobase.org/index.php?title=Streptomyces_Bacteriophages&diff=1679&oldid=prev
MFeeney at 08:25, 5 May 2020
2020-05-05T08:25:34Z
<p></p>
<table style="background-color: #fff; color: #202122;" data-mw="interface">
<col class="diff-marker" />
<col class="diff-content" />
<col class="diff-marker" />
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 08:25, 5 May 2020</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>The model Streptomyces phage (our equivalent of the model coliphage, λ) is the temperate phage [[ΦC31]], with clear plaque (lytic) mutants (cΔ25) available and often used in the laboratory. The R4-like group of phages appear to be the most abundant Streptomyces phages globally<sup>[1]</sup>. Beyond the Streptomycetes there have been limited investigations of phage within the Actinobacteria - the exception being the extensive work on Mycobacteriophages<sup>[10,13,14]</sup> and Corynephages<sup>[15]</sup>.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>The model Streptomyces phage (our equivalent of the model coliphage, λ) is the temperate phage [[ΦC31]], with clear plaque (lytic) mutants (cΔ25) available and often used in the laboratory. The R4-like group of phages appear to be the most abundant Streptomyces phages globally<sup>[1]</sup>. Beyond the Streptomycetes there have been limited investigations of phage within the Actinobacteria - the exception being the extensive work on Mycobacteriophages<sup>[10,13,14]</sup> and Corynephages<sup>[15]</sup>.</div></td></tr>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>==Protocols==</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>==Protocols==</div></td></tr>
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MFeeney
https://actinobase.org/index.php?title=Streptomyces_Bacteriophages&diff=1678&oldid=prev
MFeeney at 08:18, 5 May 2020
2020-05-05T08:18:16Z
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 08:18, 5 May 2020</td>
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<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>Streptomyces bacteriophages</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">=</ins>Streptomyces bacteriophages<ins style="font-weight: bold; text-decoration: none;">=</ins></div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>Prof Paul A Hoskisson, University of Strathclyde.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>Prof Paul A Hoskisson, University of Strathclyde.</div></td></tr>
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MFeeney