Streptomyces coelicolor A3(2) - Revision history

Matt Hutchings at 07:33, 21 October 2021

2021-10-21T07:33:31Z

← Older revision		Revision as of 07:33, 21 October 2021
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	===Links===		===Links===
	=====Genomes=====		=====Genomes=====
	Browse the ''S. coelicolor'' A3(2) genome and antiSMASH output via the [https://~~detoxbase~~.org/projects/Microbiology/ActinoBase/genome/GCF_000203835.1/view ActinoBase MORF Genome Browser].		Browse the ''S. coelicolor'' A3(2) genome and antiSMASH output via the [https://morf-db.org/projects/Microbiology/ActinoBase/genome/GCF_000203835.1/view ActinoBase MORF Genome Browser].

	=====Transcriptomes=====		=====Transcriptomes=====

Sam Prudence at 15:46, 20 February 2020

2020-02-20T15:46:39Z

← Older revision		Revision as of 15:46, 20 February 2020
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	===Links===		===Links===
	=====Genomes=====		=====Genomes=====
	Browse the ''S. coelicolor'' A3(2) genome and antiSMASH output via the [https://detoxbase.org/projects/Microbiology/ActinoBase/genome/GCF_000203835.1/view ~~ActioBase~~ MORF Genome Browser].		Browse the ''S. coelicolor'' A3(2) genome and antiSMASH output via the [https://detoxbase.org/projects/Microbiology/ActinoBase/genome/GCF_000203835.1/view ActinoBase MORF Genome Browser].

	=====Transcriptomes=====		=====Transcriptomes=====

Sam Prudence: /* Genomes */

2020-02-20T15:46:08Z

Genomes

← Older revision		Revision as of 15:46, 20 February 2020
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	===Links===		===Links===
	=====Genomes=====		=====Genomes=====
			Browse the ''S. coelicolor'' A3(2) genome and antiSMASH output via the [https://detoxbase.org/projects/Microbiology/ActinoBase/genome/GCF_000203835.1/view ActioBase MORF Genome Browser].

	=====Transcriptomes=====		=====Transcriptomes=====
	=====Proteomics=====		=====Proteomics=====

MFeeney at 09:28, 9 September 2019

2019-09-09T09:28:58Z

← Older revision		Revision as of 09:28, 9 September 2019
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	====Superhosts====		====Superhosts====

	<em>S. coelicolor</em> 'superhost' strains were engineered to help increase expression of a heterologous gene or pathway by deleting certain pathways in the native host to decrease competition of nutrients and resources <sup>[2]</sup>. The strain M1146 lacks four biosynthetic gene clusters for the production of secondary metabolites. M1152 additionally has mutations in ''rpoB,'' which encodes the β subunit of RNA polymerase, and ''rpsL,'' which encodes ribosomal protein S12~~, which~~ have been shown to increase the production of antibiotics.		<em>S. coelicolor</em> 'superhost' strains were engineered to help increase expression of a heterologous gene or pathway by deleting certain pathways in the native host to decrease competition of nutrients and resources <sup>[2]</sup>. The strain M1146 lacks four biosynthetic gene clusters for the production of secondary metabolites. M1152 additionally has mutations in ''rpoB,'' which encodes the β subunit of RNA polymerase, and ''rpsL,'' which encodes ribosomal protein S12; these mutations have been shown to increase the production of antibiotics.

	*<em>S. coelicolor</em> M1146: Δ''act'' Δ''red'' Δ''cpk'' Δ''cda''		*<em>S. coelicolor</em> M1146: Δ''act'' Δ''red'' Δ''cpk'' Δ''cda''

MFeeney at 09:28, 9 September 2019

2019-09-09T09:28:02Z

← Older revision		Revision as of 09:28, 9 September 2019
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	<em>S. coelicolor</em> 'superhost' strains were engineered to help increase expression of a heterologous gene or pathway by deleting certain pathways in the native host to decrease competition of nutrients and resources <sup>[2]</sup>. The strain M1146 lacks four biosynthetic gene clusters for the production of secondary metabolites. M1152 additionally has mutations in ''rpoB,'' which encodes the β subunit of RNA polymerase, and ''rpsL,'' which encodes ribosomal protein S12, which have been shown to increase the production of antibiotics.		<em>S. coelicolor</em> 'superhost' strains were engineered to help increase expression of a heterologous gene or pathway by deleting certain pathways in the native host to decrease competition of nutrients and resources <sup>[2]</sup>. The strain M1146 lacks four biosynthetic gene clusters for the production of secondary metabolites. M1152 additionally has mutations in ''rpoB,'' which encodes the β subunit of RNA polymerase, and ''rpsL,'' which encodes ribosomal protein S12, which have been shown to increase the production of antibiotics.

	<em>S. coelicolor ~~M1146~~</em>		*<em>S. coelicolor</em> M1146: Δ''act'' Δ''red'' Δ''cpk'' Δ''cda''
			*<em>S. coelicolor</em> M1152: Δ''act'' Δ''red'' Δ''cpk'' Δ''cda'' <em>rpoB</em>[C1298T] <em>rpsL</em>[A262G C271T]
	Δ''act'' Δ''red'' Δ''cpk'' Δ''cda''

	<em>S. coelicolor ~~M1152~~</em>

	Δ''act'' Δ''red'' Δ''cpk'' Δ''cda'' <em>rpoB</em>[C1298T] <em>rpsL</em>[A262G C271T]

	===Links===		===Links===

MFeeney at 09:26, 9 September 2019

2019-09-09T09:26:45Z

← Older revision		Revision as of 09:26, 9 September 2019
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	===Background===		===Background===

	<em>Streptomyces coelicolor</em> A3(2) is a model organism for <em>Streptomyces</em> research. It was the first Streptomycete to have its [https://www.nature.com/articles/417141a genome sequenced] in 2002, <sup>[1]</sup>. The genome has a size of 8667507 bp with more than 20 predicted gene clusters for secondary metabolites. Two of these secondary metabolites are notable for being pigmented, (which has facilitated genetic screens for mutations that affect production of these metabolites): <em> S. coelicolor </em> produces a characteristic blue pigment due to the production of the secondary metabolite actinorhodin (Act), as well as a red pigment due to the production of undecylprodigiosin (Red).		<em>Streptomyces coelicolor</em> A3(2) is a model organism for <em>Streptomyces</em> research. It was the first Streptomycete to have its [https://www.nature.com/articles/417141a genome sequenced] in 2002<sup>[1]</sup>. The genome has a size of 8667507 bp with more than 20 predicted gene clusters for secondary metabolites. Two of these secondary metabolites are notable for being pigmented, (which has facilitated genetic screens for mutations that affect production of these metabolites): <em> S. coelicolor </em> produces a characteristic blue pigment due to the production of the secondary metabolite actinorhodin (Act), as well as a red pigment due to the production of undecylprodigiosin (Red).

	===Growth & Maintenance===		===Growth & Maintenance===
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	====Superhosts====		====Superhosts====

	<em>S. coelicolor</em> 'superhost' strains were engineered to help increase expression of a heterologous gene or pathway by deleting certain pathways in the native host to decrease competition of nutrients and resources <sup>[2]</sup>. The strain M1146 lacks four biosynthetic gene clusters for the production of secondary metabolites. M1152 additionally has mutations in ''rpoB,'' which encodes the &~~Beta~~; subunit of RNA polymerase, and ''rpsL,'' which encodes ribosomal protein S12, which have been shown to increase the production of antibiotics.		<em>S. coelicolor</em> 'superhost' strains were engineered to help increase expression of a heterologous gene or pathway by deleting certain pathways in the native host to decrease competition of nutrients and resources <sup>[2]</sup>. The strain M1146 lacks four biosynthetic gene clusters for the production of secondary metabolites. M1152 additionally has mutations in ''rpoB,'' which encodes the β subunit of RNA polymerase, and ''rpsL,'' which encodes ribosomal protein S12, which have been shown to increase the production of antibiotics.

	<em>S. coelicolor M1146</em>		<em>S. coelicolor M1146</em>

MFeeney at 09:25, 9 September 2019

2019-09-09T09:25:07Z

← Older revision		Revision as of 09:25, 9 September 2019
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	~~==Organism Page==~~		== ''Streptomyces coelicolor'' A3(2) ==

	=== ''Streptomyces coelicolor'' A3(2) ===

	===Background===		===Background===

	<em>Streptomyces coelicolor</em> A3(2) is a model organism for <em>Streptomyces</em> research. It was the first Streptomycete to have its ~~genome sequenced in 2002, reported~~ [https://www.nature.com/articles/417141a ~~here~~]. The genome has a size of 8667507 bp with more than 20 predicted gene clusters for secondary metabolites. <em> S. coelicolor </em> produces a characteristic blue pigment due to the production of the secondary metabolite actinorhodin (Act), as well as a red pigment due to the production of undecylprodigiosin (Red).		<em>Streptomyces coelicolor</em> A3(2) is a model organism for <em>Streptomyces</em> research. It was the first Streptomycete to have its [https://www.nature.com/articles/417141a genome sequenced] in 2002, <sup>[1]</sup>. The genome has a size of 8667507 bp with more than 20 predicted gene clusters for secondary metabolites. Two of these secondary metabolites are notable for being pigmented, (which has facilitated genetic screens for mutations that affect production of these metabolites): <em> S. coelicolor </em> produces a characteristic blue pigment due to the production of the secondary metabolite actinorhodin (Act), as well as a red pigment due to the production of undecylprodigiosin (Red).

	===Growth & Maintenance===		===Growth & Maintenance===

	<em> S. coelicolor </em> grows well on SFM agar and in TSB liquid medium.		<em> S. coelicolor </em> grows well on [[SFM]] agar and in [[TSB]] liquid medium.

	=====Sporulation=====		=====Sporulation=====

	<em> S. coelicolor </em> sporulates well on SFM.		<em> S. coelicolor </em> sporulates well on [[SFM]] and [[IMA]].

	===Strains===		===Strains===
	====Superhosts====		====Superhosts====

	<em>S. coelicolor</em> ~~superhosts~~ were engineered to help increase expression of a heterologous gene or pathway by deleting certain pathways in the native host to decrease competition of nutrients and resources.		<em>S. coelicolor</em> 'superhost' strains were engineered to help increase expression of a heterologous gene or pathway by deleting certain pathways in the native host to decrease competition of nutrients and resources <sup>[2]</sup>. The strain M1146 lacks four biosynthetic gene clusters for the production of secondary metabolites. M1152 additionally has mutations in ''rpoB,'' which encodes the Β subunit of RNA polymerase, and ''rpsL,'' which encodes ribosomal protein S12, which have been shown to increase the production of antibiotics.

	<em>S. coelicolor M1146</em>		<em>S. coelicolor M1146</em>
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	<em>S. coelicolor M1152</em>		<em>S. coelicolor M1152</em>

	Δ''act'' Δ''red'' Δ''cpk'' Δ''cda'' <em>~~rpo~~</em>B[C1298T] <em>~~rps~~</em>L[A262G C271T]		Δ''act'' Δ''red'' Δ''cpk'' Δ''cda'' <em>rpoB</em>[C1298T] <em>rpsL</em>[A262G C271T]

	===Links===		===Links===
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	====Availability====		====Availability====
	=====Culture Collections=====		=====Culture Collections=====

			===References===

			[1] Bentley, S. D., Chater, K. F., Cerdeño-Tárraga, A. M., Challis, G. L., Thomson, N. R., James, K. D., . . . Hopwood, D. A. (2002). Complete genome sequence of the model actinomycete ''Streptomyces coelicolor'' A3(2). Nature, 417(6885), 141-147. doi:10.1038/417141a

			[2] Gomez‐Escribano, J. P. and Bibb, M. J. (2011), Engineering ''Streptomyces coelicolor'' for heterologous expression of secondary metabolite gene clusters. Microbial Biotechnology, 4: 207-215. doi:10.1111/j.1751-7915.2010.00219.x

MFeeney at 08:57, 9 September 2019

2019-09-09T08:57:38Z

← Older revision		Revision as of 08:57, 9 September 2019
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	==Organism Page==		==Organism Page==

	=== ''Streptomyces coelicolor A3(2)'' ===		=== ''Streptomyces coelicolor'' A3(2) ===

	===Background===		===Background===
Line 9:		Line 9:
	===Growth & Maintenance===		===Growth & Maintenance===

	<em> S. coelicolor </em> grows well on SFM agar and TSB liquid medium.		<em> S. coelicolor </em> grows well on SFM agar and in TSB liquid medium.

	=====Sporulation=====		=====Sporulation=====
Line 18:		Line 18:
	====Superhosts====		====Superhosts====

	<em>~~Streptomyces~~ coelicolor</em> superhosts were engineered to help increase expression of a heterologous gene or pathway by deleting certain pathways in the native host to decrease competition of nutrients and resources.		<em>S. coelicolor</em> superhosts were engineered to help increase expression of a heterologous gene or pathway by deleting certain pathways in the native host to decrease competition of nutrients and resources.

	<em>~~Streptomyces~~ coelicolor M1146</em>		<em>S. coelicolor M1146</em>

	Δact Δred Δcpk Δcda		Δ''act'' Δ''red'' Δ''cpk'' Δ''cda''

	<em>~~Streptomyces~~ coelicolor M1152</em>		<em>S. coelicolor M1152</em>

	Δact Δred Δcpk Δcda <em>rpo</em>B[C1298T] <em>rps</em>L[A262G C271T]		Δ''act'' Δ''red'' Δ''cpk'' Δ''cda'' <em>rpo</em>B[C1298T] <em>rps</em>L[A262G C271T]

	===Links===		===Links===

Alicia: /* Background */

2019-08-01T16:10:15Z

Background

← Older revision		Revision as of 16:10, 1 August 2019
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	===Background===		===Background===

	<em>Streptomyces coelicolor</em> A3(2) is a model organism for <em>Streptomyces</em> research. It was the first Streptomycete to have its genome sequenced in 2002, reported [https://www.nature.com/articles/417141a here]. The genome has a size of 8667507 bp with more than 20 predicted gene clusters for secondary metabolites. <em> S. coelicolor </em> produces a characteristic blue pigment due to the production of the secondary metabolite actinorhodin.		<em>Streptomyces coelicolor</em> A3(2) is a model organism for <em>Streptomyces</em> research. It was the first Streptomycete to have its genome sequenced in 2002, reported [https://www.nature.com/articles/417141a here]. The genome has a size of 8667507 bp with more than 20 predicted gene clusters for secondary metabolites. <em> S. coelicolor </em> produces a characteristic blue pigment due to the production of the secondary metabolite actinorhodin (Act), as well as a red pigment due to the production of undecylprodigiosin (Red).

	===Growth & Maintenance===		===Growth & Maintenance===

Alicia: /* Superhosts */

2019-08-01T16:05:23Z

Superhosts

← Older revision		Revision as of 16:05, 1 August 2019
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	<em>Streptomyces coelicolor M1146</em>		<em>Streptomyces coelicolor M1146</em>

	Δact Δred Δcpk Δcda		Δact Δred Δcpk Δcda

	<em>Streptomyces coelicolor M1152</em>		<em>Streptomyces coelicolor M1152</em>

	Δact Δred Δcpk Δcda <em>rpo</em>B[C1298T] <em>rps</em>L[A262G C271T]		Δact Δred Δcpk Δcda <em>rpo</em>B[C1298T] <em>rps</em>L[A262G C271T]