Streptomyces venezuelae - Revision history

Matt Hutchings: /* Background */

2024-06-07T08:58:04Z

Background

← Older revision		Revision as of 08:58, 7 June 2024
Line 7:		Line 7:
	There has been some confusion over the multiple iterations of the ''Streptomyces venezuelae'' genome sequence (sve, sven_15 and vnz) generated at the John Innes Centre. To clarify, all the ''Streptomyces venezuelae'' work at JIC has been performed with the same strain. The final and complete (vnz) genome sequence of this strain has been determined and submitted to the NCBI database under reference NZ_CP018074.1. The sequenced strain has also been deposited with the NRRL culture collection under stock code NRRL B-65442. Please use the NZ_CP018074.1 genome sequence and vnz gene identifiers.		There has been some confusion over the multiple iterations of the ''Streptomyces venezuelae'' genome sequence (sve, sven_15 and vnz) generated at the John Innes Centre. To clarify, all the ''Streptomyces venezuelae'' work at JIC has been performed with the same strain. The final and complete (vnz) genome sequence of this strain has been determined and submitted to the NCBI database under reference NZ_CP018074.1. The sequenced strain has also been deposited with the NRRL culture collection under stock code NRRL B-65442. Please use the NZ_CP018074.1 genome sequence and vnz gene identifiers.

	For a more complete explanation, please see Gomez-Escribano et al. (2021), Streptomyces venezuelae NRRL B-65442: genome sequence of a model strain used to study morphological differentiation in filamentous actinobacteria. [J. Ind. Microbiol. Biotechnol. 48(9-10):kuab035]. Please note that Streptomyces venezuelae NRRL B-65442 carries a 158-kb linear plasmid (vnz pvnz here in StrepDB; NCBI reference NZ_CP018075.1) and this was not assembled in the first iteration of the genome sequence (sve).		For a more complete explanation, please see Gomez-Escribano et al. (2021), ''Streptomyces venezuelae'' NRRL B-65442: genome sequence of a model strain used to study morphological differentiation in filamentous actinobacteria. [J. Ind. Microbiol. Biotechnol. 48(9-10):kuab035]. Please note that ''Streptomyces venezuelae'' NRRL B-65442 carries a 158-kb linear plasmid (vnz pvnz here in StrepDB; NCBI reference NZ_CP018075.1) and this was not assembled in the first iteration of the genome sequence (sve).

	In the NRRL culture collection database, you will see that it says that the origin of NRRL B-65442 is "unsure". This is because we cannot unambiguously trace the continuous lineage of the JIC strain back to the 1940s. However, its genome sequence is more than 99.999% identical to ATCC10712, and so it is clearly descended from the Caracas/Yale/Burkholder strain (Ehrlich et al 1947. Science 106: 417; Ehrlich et al. 1948. J Bacteriol 56: 467-477) and not from the Illinois Agricultural Experiment Station at Urbana/Gottlieb strain (Gottlieb et al. 1948. J Bacteriol 55: 409-17). If you wish to work with this strain it can be obtained directly from the NRRL culture collection (stock code NRRL B-65442) or by emailing jic.strepstrains@jic.ac.uk. If you have obtained S. venezuelae from the John Innes Centre, then you already have NRRL B-65442.		In the NRRL culture collection database, you will see that it says that the origin of NRRL B-65442 is "unsure". This is because we cannot unambiguously trace the continuous lineage of the JIC strain back to the 1940s. However, its genome sequence is more than 99.999% identical to ATCC10712, and so it is clearly descended from the Caracas/Yale/Burkholder strain (Ehrlich et al 1947. Science 106: 417; Ehrlich et al. 1948. J Bacteriol 56: 467-477) and not from the Illinois Agricultural Experiment Station at Urbana/Gottlieb strain (Gottlieb et al. 1948. J Bacteriol 55: 409-17).

			If you wish to work with this strain it can be obtained directly from the NRRL culture collection (stock code NRRL B-65442) or by emailing jic.strepstrains@jic.ac.uk. If you have obtained ''S. venezuelae'' from the John Innes Centre, then you already have NRRL B-65442.

	===Growth & Maintenance===		===Growth & Maintenance===

Matt Hutchings: /* Background */

2024-06-07T08:57:22Z

Background

← Older revision		Revision as of 08:57, 7 June 2024
Line 5:		Line 5:
	===Background===		===Background===

	There has been some confusion over the multiple iterations of the Streptomyces venezuelae genome sequence (sve, sven_15 and vnz) generated at the John Innes Centre. To clarify, all the Streptomyces venezuelae work at JIC has been performed with the same strain. The final and complete (vnz) genome sequence of this strain has been determined and submitted to the NCBI database under reference NZ_CP018074.1. The sequenced strain has also been deposited with the NRRL culture collection under stock code NRRL B-65442. Please use the NZ_CP018074.1 genome sequence and vnz gene identifiers.		There has been some confusion over the multiple iterations of the ''Streptomyces venezuelae'' genome sequence (sve, sven_15 and vnz) generated at the John Innes Centre. To clarify, all the ''Streptomyces venezuelae'' work at JIC has been performed with the same strain. The final and complete (vnz) genome sequence of this strain has been determined and submitted to the NCBI database under reference NZ_CP018074.1. The sequenced strain has also been deposited with the NRRL culture collection under stock code NRRL B-65442. Please use the NZ_CP018074.1 genome sequence and vnz gene identifiers.

	For a more complete explanation, please see Gomez-Escribano et al. (2021), Streptomyces venezuelae NRRL B-65442: genome sequence of a model strain used to study morphological differentiation in filamentous actinobacteria. [J. Ind. Microbiol. Biotechnol. 48(9-10):kuab035]. Please note that Streptomyces venezuelae NRRL B-65442 carries a 158-kb linear plasmid (vnz pvnz here in StrepDB; NCBI reference NZ_CP018075.1) and this was not assembled in the first iteration of the genome sequence (sve).		For a more complete explanation, please see Gomez-Escribano et al. (2021), Streptomyces venezuelae NRRL B-65442: genome sequence of a model strain used to study morphological differentiation in filamentous actinobacteria. [J. Ind. Microbiol. Biotechnol. 48(9-10):kuab035]. Please note that Streptomyces venezuelae NRRL B-65442 carries a 158-kb linear plasmid (vnz pvnz here in StrepDB; NCBI reference NZ_CP018075.1) and this was not assembled in the first iteration of the genome sequence (sve).

	In the NRRL culture collection database, you will see that it says that the origin of NRRL B-65442 is "unsure". This is because we cannot unambiguously trace the continuous lineage of the JIC strain back to the 1940s. However, its genome sequence is more than 99.999% identical to ATCC10712, and so it is clearly descended from the Caracas/Yale/Burkholder strain (Ehrlich et al 1947. Science 106: 417; Ehrlich et al. 1948. J Bacteriol 56: 467-477) and not from the Illinois Agricultural Experiment Station at Urbana/Gottlieb strain (Gottlieb et al. 1948. J Bacteriol 55: 409-17). If you wish to work with this strain it can be obtained directly from the NRRL culture collection (stock code NRRL B-65442) or by emailing jic.strepstrains@jic.ac.uk. If you have obtained S. venezuelae from the John Innes Centre, then you already have NRRL B-65442.		In the NRRL culture collection database, you will see that it says that the origin of NRRL B-65442 is "unsure". This is because we cannot unambiguously trace the continuous lineage of the JIC strain back to the 1940s. However, its genome sequence is more than 99.999% identical to ATCC10712, and so it is clearly descended from the Caracas/Yale/Burkholder strain (Ehrlich et al 1947. Science 106: 417; Ehrlich et al. 1948. J Bacteriol 56: 467-477) and not from the Illinois Agricultural Experiment Station at Urbana/Gottlieb strain (Gottlieb et al. 1948. J Bacteriol 55: 409-17). If you wish to work with this strain it can be obtained directly from the NRRL culture collection (stock code NRRL B-65442) or by emailing jic.strepstrains@jic.ac.uk. If you have obtained S. venezuelae from the John Innes Centre, then you already have NRRL B-65442.



	===Growth & Maintenance===		===Growth & Maintenance===

Matt Hutchings at 08:55, 7 June 2024

2024-06-07T08:55:43Z

← Older revision		Revision as of 08:55, 7 June 2024
Line 4:		Line 4:

	===Background===		===Background===

			There has been some confusion over the multiple iterations of the Streptomyces venezuelae genome sequence (sve, sven_15 and vnz) generated at the John Innes Centre. To clarify, all the Streptomyces venezuelae work at JIC has been performed with the same strain. The final and complete (vnz) genome sequence of this strain has been determined and submitted to the NCBI database under reference NZ_CP018074.1. The sequenced strain has also been deposited with the NRRL culture collection under stock code NRRL B-65442. Please use the NZ_CP018074.1 genome sequence and vnz gene identifiers.

			For a more complete explanation, please see Gomez-Escribano et al. (2021), Streptomyces venezuelae NRRL B-65442: genome sequence of a model strain used to study morphological differentiation in filamentous actinobacteria. [J. Ind. Microbiol. Biotechnol. 48(9-10):kuab035]. Please note that Streptomyces venezuelae NRRL B-65442 carries a 158-kb linear plasmid (vnz pvnz here in StrepDB; NCBI reference NZ_CP018075.1) and this was not assembled in the first iteration of the genome sequence (sve).

			In the NRRL culture collection database, you will see that it says that the origin of NRRL B-65442 is "unsure". This is because we cannot unambiguously trace the continuous lineage of the JIC strain back to the 1940s. However, its genome sequence is more than 99.999% identical to ATCC10712, and so it is clearly descended from the Caracas/Yale/Burkholder strain (Ehrlich et al 1947. Science 106: 417; Ehrlich et al. 1948. J Bacteriol 56: 467-477) and not from the Illinois Agricultural Experiment Station at Urbana/Gottlieb strain (Gottlieb et al. 1948. J Bacteriol 55: 409-17). If you wish to work with this strain it can be obtained directly from the NRRL culture collection (stock code NRRL B-65442) or by emailing jic.strepstrains@jic.ac.uk. If you have obtained S. venezuelae from the John Innes Centre, then you already have NRRL B-65442.



	===Growth & Maintenance===		===Growth & Maintenance===

Matt Hutchings at 07:32, 21 October 2021

2021-10-21T07:32:17Z

← Older revision		Revision as of 07:32, 21 October 2021
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	===Links===		===Links===
	=====Genomes=====		=====Genomes=====
	Browse the ''S. venezluelae'' genome and antiSMASH output via the [https://~~detoxbase~~.org/projects/Microbiology/ActinoBase/genome/GCF_001443625.1/view ~~ActinoBase~~ MORF genome browser].		Browse the ''S. venezluelae'' genome and antiSMASH output via the [https://morf-db.org/projects/Microbiology/ActinoBase/genome/GCF_001443625.1/view MORF genome browser].

	=====Transcriptomes=====		=====Transcriptomes=====

Sam Prudence: /* Genomes */

2020-02-20T15:50:46Z

Genomes

← Older revision		Revision as of 15:50, 20 February 2020
Line 15:		Line 15:
	===Links===		===Links===
	=====Genomes=====		=====Genomes=====
			Browse the ''S. venezluelae'' genome and antiSMASH output via the [https://detoxbase.org/projects/Microbiology/ActinoBase/genome/GCF_001443625.1/view ActinoBase MORF genome browser].

	=====Transcriptomes=====		=====Transcriptomes=====
	=====Proteomics=====		=====Proteomics=====

Sam Prudence at 14:48, 28 June 2019

2019-06-28T14:48:44Z

← Older revision		Revision as of 14:48, 28 June 2019
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	=====Transcriptomes=====		=====Transcriptomes=====
	=====Proteomics=====		=====Proteomics=====
			=====Protocols=====

	====Availability====		====Availability====
	=====Culture Collections=====		=====Culture Collections=====


	~~=====Protocols=====~~

Sam Prudence at 14:47, 28 June 2019

2019-06-28T14:47:47Z

← Older revision		Revision as of 14:47, 28 June 2019
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	*Sporulation can be achieved after 3-5 days growth on [[MYM]] medium (liquid or solid) containing [[trace elements]].		*Sporulation can be achieved after 3-5 days growth on [[MYM]] medium (liquid or solid) containing [[trace elements]].
	*To make stocks, follow [[Spore Stocking]] protocol, using [[MYM]] medium.		*To make stocks, follow [[Spore Stocking]] protocol, using [[MYM]] medium.

			===Strains===
			====Superhosts====

	===Links===		===Links===
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	=====Transcriptomes=====		=====Transcriptomes=====
	=====Proteomics=====		=====Proteomics=====
			====Availability====
			=====Culture Collections=====


	=====Protocols=====		=====Protocols=====

Test: /* Sporulation */

2019-05-06T16:39:35Z

Sporulation

← Older revision		Revision as of 16:39, 6 May 2019
Line 8:		Line 8:
	=====Sporulation=====		=====Sporulation=====
	*Sporulation can be achieved after 3-5 days growth on [[MYM]] medium (liquid or solid) containing [[trace elements]].		*Sporulation can be achieved after 3-5 days growth on [[MYM]] medium (liquid or solid) containing [[trace elements]].
	*To make stocks, follow [[Spore Stocking]] ~~protocolm~~, using [[MYM]] medium.		*To make stocks, follow [[Spore Stocking]] protocol, using [[MYM]] medium.

	===Links===		===Links===

Test: /* Proteome */

2019-05-06T16:36:01Z

Proteome

← Older revision		Revision as of 16:36, 6 May 2019
Line 13:		Line 13:
	=====Genomes=====		=====Genomes=====
	=====Transcriptomes=====		=====Transcriptomes=====
	=====~~Proteome~~=====		=====Proteomics=====

	=====Protocols=====		=====Protocols=====

Test: /* ==Protocols */

2019-05-06T16:35:52Z

==Protocols

← Older revision		Revision as of 16:35, 6 May 2019
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	=====Transcriptomes=====		=====Transcriptomes=====
	=====Proteome=====		=====Proteome=====
	=====Protocols===		=====Protocols=====