https://actinobase.org/index.php?action=history&feed=atom&title=Wheat_Take-all_bioassaysWheat Take-all bioassays - Revision history2026-10-17T21:02:11ZRevision history for this page on the wikiMediaWiki 1.39.3https://actinobase.org/index.php?title=Wheat_Take-all_bioassays&diff=2050&oldid=prevJake Newitt at 12:35, 28 March 20222022-03-28T12:35:56Z<p></p>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>* Glass Pasteur pipette or sterile P1000 pipette tips</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>* Glass Pasteur pipette or sterile P1000 pipette tips</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">'''</del>Instructions<del style="font-weight: bold; text-decoration: none;">'''</del></div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">==</ins>Instructions<ins style="font-weight: bold; text-decoration: none;">==</ins></div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div># Inoculate PDA plates in triplicate by spotting 5μl of ''Streptomyces'' spores about a third of the way into a plate of PDA.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div># Inoculate PDA plates in triplicate by spotting 5μl of ''Streptomyces'' spores about a third of the way into a plate of PDA.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div># Allow cultures to grow for 7 days at 30&deg;.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div># Allow cultures to grow for 7 days at 30&deg;.</div></td></tr>
</table>Jake Newitthttps://actinobase.org/index.php?title=Wheat_Take-all_bioassays&diff=2049&oldid=prevJake Newitt: Created page with "=Wheat Take-all bioassays= This assay is used to determine whether ''Streptomyces'' strains are able to produce antifungal compounds that inhibit the wheat root pathogen ''Ga..."2022-03-28T12:35:03Z<p>Created page with "=Wheat Take-all bioassays= This assay is used to determine whether ''Streptomyces'' strains are able to produce antifungal compounds that inhibit the wheat root pathogen ''Ga..."</p>
<p><b>New page</b></p><div>=Wheat Take-all bioassays=<br />
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This assay is used to determine whether ''Streptomyces'' strains are able to produce antifungal compounds that inhibit the wheat root pathogen ''Gaeumannomyces tritici'', which is causes wheat take-all disease.<br />
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This assay usually takes 14 days, but it may take longer as the time taken for the plug to establish in the assay plate may vary.<br />
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==Preparation and reagents needed==<br />
* 33ml Potato Dextrose Agar (PDA) plates.<br />
* ''Streptomyces'' spores in 20% glycerol.<br />
* Actively growing plate of ''Gaeumannomyces tritici''.<br />
* Glass Pasteur pipette or sterile P1000 pipette tips<br />
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'''Instructions'''<br />
# Inoculate PDA plates in triplicate by spotting 5μl of ''Streptomyces'' spores about a third of the way into a plate of PDA.<br />
# Allow cultures to grow for 7 days at 30&deg;.<br />
# Using a flame sterilized Pasteur pipette or P1000 pipette tip, remove a plug of agar about a third of the way into the plate opposite the ''Streptomyces'' colony. Sterile toothpicks can be used to assist removal of the agar plug.<br />
# Remove a plug of agar from actively growing ''Gaeumannomyces tritici'' and transfer it to the ''Streptomyces'' plate. It is useful to transfer plugs in triplicate to blank PDA plates as a negative control<br />
# Allow plates to grow at 22&deg; for 7 days. The time taken for the plug to establish in the assay plate may vary, so may need to be grown for longer.</div>Jake Newitt