Jake Newitt: Created page with "{{DISPLAYTITLE: Wheat seed sterilisation}} =Wheat seed sterilisation protocol= Wheat seeds are typically sterilised before growing in sterile conditions, such is the case for..."

2021-10-26T14:25:23Z

Created page with "{{DISPLAYTITLE: Wheat seed sterilisation}} =Wheat seed sterilisation protocol= Wheat seeds are typically sterilised before growing in sterile conditions, such is the case for..."

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{{DISPLAYTITLE: Wheat seed sterilisation}}
=Wheat seed sterilisation protocol=

Wheat seeds are typically sterilised before growing in sterile conditions, such is the case for [[harvesting root exudates| harvesting wheat root exudates]]. They may also be sterilized prior to inoculating with ''Streptomyces'' bacteria, which is often done to [[Root colonisation assay – colony forming unit (CFU) count method| determine root colonisation fitness]].

This protocol takes around 30 minutes

==Preparation and reagents needed==
*Ethanol 70%
*3% Bleach solution
*Sterile ddH<sub>2</sub>O
*50ml falcon tubes
*Sterile petri dishes
*Disposable gloves
*Timer

==Wheat seed sterilization protocol==
#Transfer the required number of seeds to a clean, sterile 50ml falcon tube.
#Submerge wheat seeds in 70% ethanol and incubate for 30 seconds, then pour off.
#Submerge wheat seeds in 3% bleach and incubate for 10 minutes, then pour off.
#Wash seeds in sterile ddH<sub>2</sub>O 10 times.
#If seeds are to be inoculated with ''Streptomyces'' spores, allow to dry in a sterile petri dish under sterile conditions.

Wheat seed sterilisation - Revision history

Jake Newitt: Created page with "{{DISPLAYTITLE: Wheat seed sterilisation}} =Wheat seed sterilisation protocol= Wheat seeds are typically sterilised before growing in sterile conditions, such is the case for..."