https://actinobase.org/index.php?action=history&feed=atom&title=YPD 2026-10-17T22:12:05Z Revision history for this page on the wiki MediaWiki 1.39.3 https://actinobase.org/index.php?title=YPD&diff=463&oldid=prev 2019-07-04T15:42:12Z

<p>Created page with &quot;=Yeast Peptone Dextrose= Yeast Peptone Dextrose (YPD) medium is composed of Yeast Peptone medium (YP) with the addition of dextrose (D-glucose). YPD is a general medium used...&quot;</p> <p><b>New page</b></p><div>=Yeast Peptone Dextrose=<br /> <br /> Yeast Peptone Dextrose (YPD) medium is composed of Yeast Peptone medium (YP) with the addition of dextrose (D-glucose). YPD is a general medium used for the cultivation of fungal species and investigating &#039;&#039;Streptomyces&#039;&#039; exploration &lt;sup&gt;[1]&lt;/sup&gt;. <br /> <br /> ==Preparation==<br /> Per Litre:<br /> *10g yeast extract<br /> *20g bacto-peptone<br /> *20g Agar<br /> *1L with dH&lt;sub&gt;2&lt;/sub&gt;0 water<br /> <br /> 20% dextrose:<br /> *20g dextrose<br /> *100ml dH&lt;sub&gt;2&lt;/sub&gt;0<br /> *Filter sterilise<br /> <br /> &#039;&#039;&#039;Instructions&#039;&#039;&#039;<br /> #Dissolve 10g yeast extract and 20g bacto-peptone in 1L dH&lt;sub&gt;2&lt;/sub&gt;0 making YP solution<br /> #Add 2g agar to each 250mL flask<br /> #Add 100mL YP solution to each flask<br /> #Autoclave - this makes YP agar<br /> #When cool add 10mL 20% dextrose solution to 100mL media <br /> <br /> ==Uses==<br /> *Explorer phenotype investigation<br /> *Fungal cultivation<br /> <br /> ==Notes==<br /> *YP has been shown to induce exploration in &#039;&#039;Streptomyces venezuelae&#039;&#039; whilst YPD has been shown to repress it unless co-cultivated with certain fungal strains <br /> <br /> ==References==<br /> 1. Jones S, Ho L, Rees C, Hill J, Nodwell J, Elliot M. &#039;&#039;Streptomyces&#039;&#039; exploration is triggered by fungal interactions and volatile signals. &#039;&#039;eLife&#039;&#039;. 2017;6.</div>

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