CRISPR/Cas9-mediated genome editing: Difference between revisions
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For a comprahensive guide for CRISPR/Cas knockouts in ''Streptomyces'' using pCRISPomyces-2 see the link below to the 'Hutchings Lab protocol for generating CRISPR/Cas Knockouts using pCRISPomyces-2', written by Dr. Rebecca Devine. | For a comprahensive guide for CRISPR/Cas knockouts in ''Streptomyces'' using pCRISPomyces-2 see the link below to the 'Hutchings Lab protocol for generating CRISPR/Cas Knockouts using pCRISPomyces-2', written by Dr. Rebecca Devine. | ||
[https://actinobase.org/downloads/pCRISPomyces2.pdf Hutchings Lab protocol for generating CRISPR/Cas Knockouts using pCRISPomyces-2] | |||
Revision as of 17:23, 7 November 2019
Can be used for precise CRISPR/Cas9-mediated genome engineering of Streptomyces strains. Mutations made have ranged from 1-100kbp deletions, precise codon changes to alter amino acids and insertions to add Flag-tags to proteins encoded at their native loci. Several different plasmids have been constructed and published and are listed below. Click the links to find out more.
For a comprahensive guide for CRISPR/Cas knockouts in Streptomyces using pCRISPomyces-2 see the link below to the 'Hutchings Lab protocol for generating CRISPR/Cas Knockouts using pCRISPomyces-2', written by Dr. Rebecca Devine. Hutchings Lab protocol for generating CRISPR/Cas Knockouts using pCRISPomyces-2
