CRISPR/Cas9-mediated genome editing: Difference between revisions

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CRISPR/Cas9 can be used for precise CRISPR/Cas9-mediated genome engineering of <em>Streptomyces</em> strains. Mutations made have ranged from 1-100kbp deletions, precise codon changes to alter amino acids and insertions to add Flag-tags to proteins encoded at their native loci. Several different plasmids have been constructed and published and are listed below. Click the links to find out more.  
Can be used for precise CRISPR/Cas9-mediated genome engineering of <em>Streptomyces</em> strains. Mutations made have ranged from 1-100kbp deletions, precise codon changes to alter amino acids and insertions to add Flag-tags to proteins encoded at their native loci. Several different plasmids have been constructed and published and are listed below. Click the links to find out more.  


[[pCRISPomyces2]]
[[pCRISPomyces2]]

Revision as of 13:20, 19 July 2019

Can be used for precise CRISPR/Cas9-mediated genome engineering of Streptomyces strains. Mutations made have ranged from 1-100kbp deletions, precise codon changes to alter amino acids and insertions to add Flag-tags to proteins encoded at their native loci. Several different plasmids have been constructed and published and are listed below. Click the links to find out more.

pCRISPomyces2